CN114391330A - Thermal shock method for improving coriaria sinica seed germination - Google Patents
Thermal shock method for improving coriaria sinica seed germination Download PDFInfo
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- CN114391330A CN114391330A CN202210012390.9A CN202210012390A CN114391330A CN 114391330 A CN114391330 A CN 114391330A CN 202210012390 A CN202210012390 A CN 202210012390A CN 114391330 A CN114391330 A CN 114391330A
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- seeds
- coriaria
- germination
- thermal shock
- coriaria sinica
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- 235000016061 Coriaria sinica Nutrition 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000035939 shock Effects 0.000 title claims description 20
- 230000007226 seed germination Effects 0.000 title description 6
- 230000035784 germination Effects 0.000 claims abstract description 55
- 241000218170 Coriaria Species 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 238000002635 electroconvulsive therapy Methods 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 4
- 239000012286 potassium permanganate Substances 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 239000008223 sterile water Substances 0.000 claims description 4
- 241000238631 Hexapoda Species 0.000 claims description 3
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 239000000428 dust Substances 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims 2
- 238000002791 soaking Methods 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 238000009331 sowing Methods 0.000 abstract 1
- 241000894007 species Species 0.000 description 4
- 238000001816 cooling Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 241001327690 Coriaria nepalensis Species 0.000 description 2
- 230000000249 desinfective effect Effects 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000218168 Coriariaceae Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000592344 Spermatophyta Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
The invention belongs to the technical field of plant cultivation, and discloses a treatment method for improving germination capacity of coriaria sinica seeds. The method of the invention improves the germination rate of coriaria sinica seeds from original about 75% to about 85%, shortens the germination starting time from original 15 days to 5 days, shortens the germination finishing time from 24 days to 17 days, improves the germination index from 46 to 114, and improves the germination potential from 22% to 27%, thereby greatly enhancing the germination capacity of coriaria sinica seeds. The method is simple, economic and green, and by utilizing the technology, the problems of weak germination capacity, low germination rate and long germination time of the coriaria sinica diels in field sowing can be solved, and the method has important significance for the production practice of coriaria sinica shrub seeds.
Description
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a thermal shock method for improving coriaria sinica seed germination.
Background
Coriaria nepalensis (Coriaria nepalensis) is perennial deciduous small trees or shrubs in Coriaria L of Coriaria of Coriariaceae, is mainly distributed in India, Nepal and Shanxi, Shaanxi, Hunan, Sichuan, Yunnan, Guizhou provinces of China, is usually grown in a substantial mountain land with an elevation of 400-2000 m or a dry barren slope, and is a common pioneer tree species in barren mountain wastelands. The coriaria sinica plant can be used for extracting tannin extract and pesticide, the root can be used for medicine, the seed can be used for extracting oil, the fruit can be used for extracting alcohol, the leaf can be used for fertilizer, the tender branch and leaf can be used for feed, the leaf can also be used for culturing coriaria sinica, the wood can be used as coriaria sinica mushroom bacterial material, and is also an ecological economic tree species with wide utilization prospect, and the ecological economic tree species has higher ecological value, medicinal value and economic value.
The germination of seeds is the most critical period for individual development of seed plants into plants, is directly related to smooth propagation and evolution of populations, and has extremely important biological significance. The germination of the seeds is a growth process that the seeds are viable, respiration, substance synthesis and other metabolic activities are started under the hydrothermal guarantee condition, and the embryo breaks through the seed coat and grows out of the radicle after a certain time.
At present, the direct seeding method is adopted to breed coriaria sinica maxim, and the following problems exist: the propagation and germination time of the seeds is long, the coriaria sinica is pioneer tree species, the growth environment is drought, the germination water supply is insufficient, the germination rate of coriaria sinica seeds is low and generally does not exceed 25%, and the planting of coriaria sinica is severely limited. By improving the germination capacity of coriaria sinica seeds, the problem of low germination rate under the condition of field forest land broadcasting can be solved, and the promotion of efficient utilization of coriaria sinica resources is facilitated.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a heat shock method for improving the germination capacity of coriaria seeds.
The invention is realized in such a way that the thermal shock method for improving the germination capacity of coriaria seeds comprises the following steps:
step one, seed screening: selecting seeds which are normally developed, fully mature and free from diseases and insect pests, removing impurities such as heterogeneous seeds, scraps, dust and the like, and screening full and glossy particles for later use;
step two, seed disinfection: washing seeds with warm water for 2-3 times, then putting coriaria sinica seeds and disinfecting the seeds with 0.1% potassium permanganate for 10 minutes, shaking lightly in the disinfection process to improve the disinfection effect, and washing the seeds with sterile water for 4-5 times;
step three, thermal shock treatment of seeds: and (3) putting the sterilized coriaria seeds in a constant-temperature oven at 60 ℃ for thermal shock for 20 minutes, and then taking out and naturally cooling to obtain coriaria seeds with strong germination capacity.
By combining all the technical schemes, the invention has the advantages and positive effects that: the coriaria sinica seeds are subjected to thermal shock treatment, so that the germination rate is improved from about 75% to about 85%, the germination starting time is shortened from about 15 days to about 5 days, the germination finishing time is shortened from about 24 days to about 17 days, the germination index is improved from 46 to 114, and the germination vigor is improved from 22% to 27%. The coriaria sinica seed germination capacity can be effectively improved, and the problem of low germination rate under the condition of field forest land broadcasting can be solved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a flow chart of a thermal shock method for improving germination capacity of coriaria seeds according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a heat shock method for improving the germination capacity of coriaria seeds, and the technical scheme of the invention is described in detail with reference to the attached drawings.
As shown in fig. 1, the heat shock method for improving germination capacity of coriaria seeds provided by the embodiment of the present invention comprises the following steps:
s101, seed screening: selecting seeds which are normally developed, fully mature and free from diseases and insect pests, removing impurities such as heterogeneous seeds, scraps, dust and the like, and screening full and glossy particles for later use;
s102, seed disinfection: washing seeds with warm water for 2-3 times, then putting coriaria sinica seeds and disinfecting the seeds with 0.1% potassium permanganate for 10 minutes, shaking lightly in the disinfection process to improve the disinfection effect, and washing the seeds with sterile water for 4-5 times;
s103, thermal shock treatment of seeds: and (3) putting the sterilized coriaria seeds in a constant-temperature oven at 60 ℃ for thermal shock for 20 minutes, and then taking out and naturally cooling to obtain coriaria seeds with strong germination capacity.
The heat shock method for improving germination capacity of coriaria sinica maxim seeds provided by the present invention may be implemented by other steps, and the heat shock method for improving germination capacity of coriaria sinica maxim seeds provided by the present invention in fig. 1 is only one specific example.
The technical effects of the present invention will be described in detail with reference to experiments.
1 Material
Test materials: coriaria sinica seed from agricultural science institute of Guizhou province
2 method
2.1 Experimental treatment
Selecting coriaria sinica seeds with uniform size, full particles and consistent color, respectively adopting 9 modes of 40 ℃ (20 minutes, 40 minutes, 60 minutes), 60 ℃ (10 minutes, 20 minutes, 30 minutes) and 80 ℃ (5 minutes, 10 minutes and 15 minutes) in an oven (3 culture dishes are placed in the oven, the temperature is set, after the temperature of the oven reaches the set temperature, the coriaria sinica seeds are rapidly placed in the culture dishes for high-temperature treatment, 150 seeds are placed in each culture dish to avoid overlapping, after the treatment time is reached, the coriaria sinica seeds are rapidly taken out and are transferred into a prepared normal-temperature culture dish for natural cooling), carrying out dry heat treatment, wherein each treatment is repeated for 5 times, and each treatment is carried out for 30 seeds, and the comparison is carried out at normal temperature (25 ℃).
The treated coriaria seeds were spread evenly on a 9cm petri dish with 3 layers of filter paper and moistened with 5 ml of distilled water (with appropriate water). The seeds were cultured in an illumination incubator set at 25 ℃ with 60% air humidity, with total light exposure of 12 hours per day.
2.2 index determination
Observing from the next day when the seeds are placed in a constant temperature incubator, observing the germination condition of the seeds by taking the criterion that radicles break through 1 mm (white exposed) of seed coats, observing for 1 time every 24 hours, recording data from the germination of the 1 st seeds, recording the number of the germinated coriaria seeds every day, and observing after no seeds germinate for 5 consecutive days.
2.3 characterization of seed Germination Capacity
The germination capacity of coriaria sinica seeds is characterized by 7 indexes of germination rate, germination starting time, germination ending time, germination duration time, germination index, thermal shock effect index, germination vigor and the like. The main formula is as follows:
the germination rate is the germination number/total number of tested seeds multiplied by 100 percent;
germination index ═ Σ (G)t/Dt) In the formula GtThe number of sprouts on day t, DtThe number of days of seed germination;
thermal shock effect index: RI ═ 1-C/T (when T ≧ C)
RI ═ T/C-1 (when T < C)
Wherein C is the germination rate of a control group, T is the germination rate of a treatment group, when RI is more than 0, the germination is promoted, and when RI is less than 0, the germination is inhibited. The absolute value of RI represents the intensity of action.
Germination vigor is the maximum number of seeds germinated per day/number of seeds tested x 100%.
TABLE 1 Experimental data
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (5)
1. A thermal shock method for improving germination capacity of coriaria sinica seeds is characterized by comprising the following steps:
step 1, seed screening: selecting seeds which are normally and fully developed and have no diseases and insect pests, removing heterogeneous seeds, scraps and dust impurities, and screening full and glossy particles for later use;
step 2, seed disinfection: washing the seeds with warm water, then soaking the seeds in a potassium permanganate solution, and then washing the seeds with sterile water to obtain sterilized seeds;
step 3, thermal shock treatment of seeds: and carrying out thermal shock treatment on the disinfected seeds to obtain coriaria seeds with strong germination capacity.
2. The thermal shock method for improving germination capacity of coriaria seeds of claim 1, wherein the seeds in step 2 are washed with warm water for 2 to 3 times.
3. The thermal shock method for improving germination capacity of coriaria seeds of claim 2, wherein the coriaria seeds are sterilized with 0.1% potassium permanganate for 10 minutes in step 2, and the seeds are shaken gently during the sterilization process to improve the sterilization effect.
4. The thermal shock method for improving germination capacity of coriaria seeds of claim 3, wherein the washing with sterile water in step 2 is performed 4 to 5 times.
5. The thermal shock method for improving germination capacity of coriaria seeds according to claim 1, wherein the coriaria seeds sterilized in step 3 are placed in a constant temperature oven at 60 ℃ for thermal shock for 20 minutes, and then are taken out to be naturally cooled and sown.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210012390.9A CN114391330A (en) | 2022-01-06 | 2022-01-06 | Thermal shock method for improving coriaria sinica seed germination |
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| CN202210012390.9A CN114391330A (en) | 2022-01-06 | 2022-01-06 | Thermal shock method for improving coriaria sinica seed germination |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106233869A (en) * | 2016-08-01 | 2016-12-21 | 西南林业大学 | A kind of method improving Semen Oroxyli percentage of seedgermination |
| CN106508175A (en) * | 2016-11-09 | 2017-03-22 | 西南林业大学 | Method used for increasing germination rate of capsicum frutescens |
| CN109479689A (en) * | 2018-12-14 | 2019-03-19 | 扬州大学 | A kind of epiphysin efficiently concentrating method in Vegetables in Brassica sprout |
| CN111955293A (en) * | 2020-08-26 | 2020-11-20 | 民勤县全盛永泰农业有限公司 | Seedling raising method for improving survival rate of seeds |
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- 2022-01-06 CN CN202210012390.9A patent/CN114391330A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106233869A (en) * | 2016-08-01 | 2016-12-21 | 西南林业大学 | A kind of method improving Semen Oroxyli percentage of seedgermination |
| CN106508175A (en) * | 2016-11-09 | 2017-03-22 | 西南林业大学 | Method used for increasing germination rate of capsicum frutescens |
| CN109479689A (en) * | 2018-12-14 | 2019-03-19 | 扬州大学 | A kind of epiphysin efficiently concentrating method in Vegetables in Brassica sprout |
| CN111955293A (en) * | 2020-08-26 | 2020-11-20 | 民勤县全盛永泰农业有限公司 | Seedling raising method for improving survival rate of seeds |
Non-Patent Citations (5)
| Title |
|---|
| 余娇娥 等: "萌发前高温处理对黄茅种子萌发率的影响", 《种子》 * |
| 张进国 等: "喀斯特山区乡土灌木种子吸胀与萌发特性", 《贵州农业科学》 * |
| 杨成 等: "干旱胁迫及土壤埋藏深度对马桑种子萌发的影响", 《安徽农业科学》 * |
| 阳翠等: "热击处理对黄瓜种子无菌播种萌芽的影响", 《内江师范学院学报》 * |
| 霍冬芳 等: "萌发前高温处理对坡柳种子萌发率的影响", 《种子》 * |
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Application publication date: 20220426 |