CN114376932A

CN114376932A - Composition for improving stimulation of gamma-aminobutyric acid and preparation method and application thereof

Info

Publication number: CN114376932A
Application number: CN202210181017.6A
Authority: CN
Inventors: 李薄薄; 李丽丽; 马洁; 由冰; 亓云吉
Original assignee: Shandong Huawutang Biotechnology Co ltd
Current assignee: Shandong Huawutang Biotechnology Co ltd
Priority date: 2022-02-25
Filing date: 2022-02-25
Publication date: 2022-04-22
Anticipated expiration: 2042-02-25
Also published as: CN114376932B

Abstract

The invention provides a composition for improving stimulation of gamma-aminobutyric acid, and a preparation method and application thereof. The composition comprises the gamma-aminobutyric acid, the macromolecular humectant, the micromolecular humectant and the soothing agent, and can effectively relieve instant discomfort, especially tingling, brought by the use of the gamma-aminobutyric acid through the synergistic compounding of the gamma-aminobutyric acid, the macromolecular humectant, the micromolecular humectant and the soothing agent, so that the safe dosage of the gamma-aminobutyric acid is improved. In addition, the composition has better effects of resisting wrinkles and removing wrinkles. The composition has the advantages of simple preparation, easily obtained raw materials, low cost, and convenient popularization, application and commercial production.

Description

Composition for improving stimulation of gamma-aminobutyric acid and preparation method and application thereof

Technical Field

The application relates to the field of cosmetics, in particular to a composition for improving stimulation of gamma-aminobutyric acid and a preparation method and application thereof.

Background

Wrinkle resistance, wrinkle removal and aging delay are topics which are unchangeable after the ancient times of people who love beauty. With the aging and external environmental influences, wrinkles appear on the forehead, the upper and lower eyelids, the outer canthus, the anterior auricular region, the cheek, the neck, the chin, the periphery of the mouth, etc. From ancient times to date, various methods have been tried to delay the development of wrinkles, and most of these methods have been slow in effectiveness or have received little effect.

Gamma-aminobutyric acid (GABA), also known as aminobutyric acid, 4-aminobutyric acid, pipecolic acid, having a molecular formula of C₄H₉NO₂The molecular weight is 103.1. Gamma-aminobutyric acid is an inhibitory neurotransmitter of the central nervous system of mammals, is widely distributed in prokaryotes and eukaryotes, and has been proved to have the effects of relieving anxiety, lowering blood pressure, improving sleep, removing wrinkles and whitening. When the wrinkle is removed, the gamma-aminobutyric acid can relax tight muscle nerve tissues, quickly penetrate through skin, fade fine wrinkles, enhance the relaxation function of muscles and play a role in quickly removing wrinkles.

By utilizing the property of the gamma-aminobutyric acid, a series of products such as facial masks, face creams, eye creams and the like are developed abroad and used in the wrinkle removing field, but the gamma-aminobutyric acid has certain side effect, namely uncomfortable symptoms such as tingling, numbness, itching, burning and the like can be generated when the high-concentration gamma-aminobutyric acid is contacted with the skin, and the side effect has positive correlation with the concentration of the gamma-aminobutyric acid. At present, the content of gamma-aminobutyric acid in cosmetics is recommended to be up to 5.0%. Under the limitation of the side effect of the gamma-aminobutyric acid, the product of the gamma-aminobutyric acid cannot reach the expected wrinkle removing concentration, so that the wrinkle removing effect of the gamma-aminobutyric acid and the wide application of the gamma-aminobutyric acid in the wrinkle removing field are limited to a certain extent.

Chinese patent CN105362091A discloses a composition containing gamma-aminobutyric acid and hyaluronic acid and its application in cosmetics, which, although the side effects of gamma-aminobutyric acid are slowed to some extent, has a single composition and still needs to be improved.

Disclosure of Invention

The invention aims to provide a composition containing gamma-aminobutyric acid, which slows down the side effect of the gamma-aminobutyric acid in use, especially reduces the tingling feeling, safely improves the dosage of the gamma-aminobutyric acid, and has good synergistic effect, and better effects of resisting wrinkles, removing wrinkles and preserving moisture.

On one hand, the application provides a composition for improving stimulation of gamma-aminobutyric acid, and the composition comprises 1-10 parts of gamma-aminobutyric acid, 1-20 parts of macromolecular humectant, 1-20 parts of micromolecular humectant and 0.1-10 parts of soothing agent by weight;

the molecular weight of the macromolecular humectant is 100-200 ten thousand daltons, and the molecular weight of the micromolecular humectant is less than 1000 daltons.

Further, the composition comprises 1-4 parts of gamma-aminobutyric acid, 1-5 parts of macromolecular humectant, 1-5 parts of micromolecular humectant and 1-2 parts of soothing agent; preferably, 3 parts of gamma-aminobutyric acid, 4 parts of macromolecular humectant, 4 parts of micromolecular humectant and 1 part of soothing agent.

Further, the macromolecular humectant is selected from one or more of biogel-1, oat glucan, sodium polyglutamate and cellulose sulfate; preferably, the macromolecular humectant comprises bioglycan-1, oat glucan and sodium polyglutamate; more preferably, the mass ratio of the biological sugar gum-1 to the oat glucan to the sodium polyglutamate is (1-5): (1-5): (1-10); more preferably, the mass ratio of the biological sugar gum-1, the oat glucan and the sodium polyglutamate is 1:1: 2.

further, the small molecular humectant is selected from one or more of trehalose, urea, menthol lactate, 4-tert-butylcyclohexanol, water-soluble ceramide and aloe juice; preferably, the micromolecular humectant is trehalose and urea; more preferably, the mass ratio of the trehalose to the urea is (1-10): (1-10); more preferably, the mass ratio of the trehalose to the urea is 1: 1.

further, the soothing agent is selected from one or more of asiaticoside and radix gentianae extract; more preferably, the soothing agent is asiaticoside.

The macromolecular humectant biogum-1, the sodium polyglutamate and the beta glucan play a role in slow release for permeation of gamma-aminobutyric acid, stimulation of the gamma-aminobutyric acid to skin is relieved, the micromolecular humectants urea and trehalose can permeate into the skin to rapidly expand the skin, the macromolecular humectant is matched to play a role in moisturizing, wrinkle removing and stimulation reducing, and the asiaticoside has good effects of relieving and pain relieving for the skin. The multiple effects take effect simultaneously, so that the composition can achieve better effects of resisting wrinkles and removing wrinkles while reducing side effects caused by gamma-aminobutyric acid.

In another aspect, the present application also provides a method of preparing a composition, the method comprising the steps of: the preparation method comprises the following steps: mixing and dissolving gamma-aminobutyric acid, a macromolecular humectant, a micromolecular humectant and a soothing agent by using water at the temperature of 40-70 ℃, and uniformly stirring to obtain the composition; preferably, the total amount is 100 parts by mass with water.

On the other hand, the application also provides the application of the composition or the composition obtained by the preparation method in wrinkle resistance, wrinkle removal, whitening and/or moisture retention; preferably, the composition is used for anti-wrinkle and wrinkle-removal.

In another aspect, the present application also provides a cosmetic comprising the above composition or the composition prepared by the above method.

Further, the content of the gamma-aminobutyric acid in the cosmetic is 0.1-15% by mass, preferably 0.5-15% by mass, and more preferably 5.1-15% by mass.

Further, the cosmetic is a mask, a cream, an emulsion, an essence, a lotion, an eye cream and/or an introduction solution.

Wherein, the sodium polyglutamate is an amino acid anion polymer with viscosity, which is discovered from natto at first, and is also called as gamma-PGA, has excellent skin care and maintenance efficacies, and promotes the synthesis of natural skin moisturizing factor PCA; inhibiting tyrosinase activity and reducing melanin synthesis; the fibroblast is activated to synthesize collagen and elastin, so that the skin is elastic, more moist and bright. The gamma-PGA can be used for constructing an embedding sustained-release system, promoting skin absorption, forming a film on the surface of the skin, isolating environmental stimulation and reducing skin inflammation.

Biosaccharide gum-1 is a linear biopolymer ((R))>10⁶Daltons). The biological carbohydrate gum-1 has strong water binding capacity, has good water absorption effect even in a low-humidity environment, and forms a breathable sugar film on the surface layer of the skin. Preventing the skin moisture from evaporating again, and simultaneously having instant and long-acting moisturizing effect. The cosmetic not only has the effects of moisturizing and relieving, but also gives soft and smooth skin feeling to the cosmetic product, and provides more choices of high-quality raw materials for a cosmetic formulator.

Oat glucan can penetrate into skin, and has obvious penetration phenomenon to both horny layer and epidermal layer. The penetration method of oat glucan is not to penetrate cells directly, but to penetrate into the skin through intercellular spaces. As water penetrates into the brick wall, it does not penetrate through the brick body, but through the concrete connecting the brick bodies. Oat glucan permeates into the skin lining through intercellular lipid matrix, also called intercellular concrete. This is in good balance with the "brick mud model" theory of skin. Since oat glucan can promote the proliferation of Langerhans cells so as to enhance the immunocompetence of the skin, clinical researches also show that the oat glucan also has remarkable anti-inflammatory and anti-allergic activities and can help the skin to resist various exogenous mechanical and chemical stimuli. Oat glucan skin activator is a highly purified cell extract, and oat glucan is actually recognized by receptors on immunocompetent cell membranes and activates immunocompetent cells. Oat glucan increases Epidermal Growth Factor (EGF) on aged skin and wrinkled skin, and promotes increase of collagen and elastin in skin, thereby improving skin appearance and removing wrinkles. The skin activator is a highly purified cell extract, and the fact that oat glucan is recognized by receptors on immunocompetent cell membranes and activates immunocompetent cells. Oat glucan increases Epidermal Growth Factor (EGF) on aged skin and wrinkled skin, and promotes increase of collagen and elastin in skin, thereby improving skin appearance and removing wrinkles.

Asiaticoside belongs to pentacyclic triterpenoid, and mainly comprises asiaticoside, madecassoside, asiatic acid, a few trace triterpenoid saponins and the like. Has various pharmacological effects of resisting ulcer, promoting wound healing, resisting tumor, resisting inflammation, regulating immunity, etc. The Mingzhijun and the like find that the asiaticoside 12, 6 and 3mg/kg can prolong the pain threshold reaction time of the mice to thermal stimulation and reduce the frequency of writhing reaction to glacial acetic acid. The asiaticoside is proved to have analgesic effect (reference document: Mingzhi, Sunpeng. asiaticoside has analgesic effect on mouse [ J ] Chinese medicine, 2001,29(6): 2.). Octopun et al observed the effect of asiaticoside on the induction of hyperalgesia by ip Lipopolysaccharide (LPS) in mice. Compared with a model group, 45 mg/kg, 15 mg/kg and 5mg/kg of asiaticoside can obviously reduce the times of mouse writhing reaction, improve pain threshold, and obviously reduce the MPO activity, NO content and plasma TNF-alpha content of brain tissues (P is less than 0.01 and 0.05) (references: octopine, Wanjingdan, Luofingling, and the like.) the asiaticoside has the effects of sensitizing the mouse lipopolysaccharide to pain sense and influencing MPO, NO and TNF-alpha [ J ] Chinese pharmaceutical journal, 2008,43(10):3 ]. The above studies suggest that asiaticoside, asiaticoside and madecassoside all have analgesic effects, and the action mechanisms of the latter two monomeric components may be related to the inhibition of inflammatory reaction.

Urea is the final product of protein metabolism and is a physiological substance of the human body. The total daily excretion amount of human is about 30 g. Wherein the urea content of the skin is about 1%. Urea affects the ability of the stratum corneum to bind moisture, is one of the natural moisturizing factors, and also has keratolytic, penetration-promoting, antimicrobial and anti-pruritic effects.

Trehalose is a non-reducing disaccharide commonly existing in organisms in the nature, is formed by connecting two molecules of pyranoglucose ring through alpha, alpha-1, 1-glycosidic bond, and has very stable chemical properties. 1, 2-trehalose has unique and excellent biological protective properties different from other disaccharides, and is capable of effectively protecting biomacromolecule structures from being damaged under extremely severe conditions (high temperature, extreme pH, high osmotic pressure, dryness, etc.), thereby maintaining the life process of organisms. The moisture retention rate reduction rate of trehalose under two relative humidities (62% and 85%) is close to that of glycerin, so that the relative moisture retention Rate (RME) of trehalose under two relative humidities is relatively stable, and therefore when a moisture retention agent with the moisture retention capacity similar to that of glycerin is needed, the defect of water absorption to skin is avoided, and trehalose with a proper concentration can be selected; the results show that RME of trehalose at 62% RH (relative humidity) for 4h is highest in 5 samples (49.13), with significant differences from aloe vera juice, biological factors, hyaluronic acid, ceramide. There were also significant differences in RME of aloe juice, biological factors, whereas the relative moisture retention rate differences of hyaluronic acid and ceramide were not significantly 10.54 and 10.06, respectively. According to the significance difference, the moisture retention performance is arranged from high to low as: trehalose, aloe juice, biological factor, hyaluronic acid, ceramide (reference: Qinlong, Wangchang, He clever, etc.. evaluation of in vitro moisturizing function of single moisturizer [ J ] daily chemical industry, 2006,36(3): 3.).

Optionally, the active ingredients of the composition of the present application may be only γ -aminobutyric acid and the macromolecular humectant, the small molecular humectant and the soothing agent, or some other active ingredients may be added based on the γ -aminobutyric acid and the macromolecular humectant, the small molecular humectant and the soothing agent to enhance the effects of anti-wrinkle, moisturizing, whitening, repairing, etc., such as small molecular peptides, collagen, betaine, squalane, trehalose, butylene glycol, complex amino acids, glycerol, creatine, tranexamic acid, D-panthenol, niacinamide, ferulic acid, lipoic acid, plant extracts, chitosan, chondroitin sulfate, heparin, ceramide, lecithin, vaseline, stearic acid, arachidic acid, hexadecyl ester, etc.; the composition can also be added with other effective components with other functions disclosed in the prior art to increase the diversity of the efficacy of the composition, for example, freckle removing effective components (specifically, tranexamic acid, arbutin, kojic acid, ellagic acid, ruscin, vitamin C and derivatives thereof, placenta and the like can be selected), acne removing effective components (specifically, tretinoin, glycolic acid, salicylic acid, malic acid, citric acid and the like can be selected), anti-allergy effective components (specifically, D-panthenol, bisabolol, glycyrrhetinic acid, allantoin, purslane extract, dipotassium glycyrrhizinate, vitamin E, plant extracts and the like can be selected), and other skin care effective components which are disclosed in the prior art and can be compounded with gamma-aminobutyric acid to achieve the synergistic effect.

The invention has the following beneficial effects:

1. experiments prove that the composition can effectively relieve the discomfort, especially the sting feeling, caused by the use of the gamma-aminobutyric acid by the synergistic compounding of the sodium polyglutamate, the biological carbohydrate gum-1, the oat glucan, the trehalose, the urea and the asiaticoside, overcomes the adverse stimulation of the high-concentration gamma-aminobutyric acid on the skin by the single use of the gamma-aminobutyric acid, and provides a new compounding combination for the gamma-aminobutyric acid.

2. The composition improves the safe use content of the gamma-aminobutyric acid in the cosmetics, and improves the content of the gamma-aminobutyric acid in the cosmetics to 5.1-15% in percentage by mass.

3. The components in the composition have good synergistic effect, the anti-wrinkle and wrinkle-removing effects of the composition are improved while the side effect of the gamma-aminobutyric acid is effectively relieved, and a novel efficient anti-wrinkle and wrinkle-removing composition is provided.

4. The composition is suitable for preparing various cosmetics such as facial masks, face creams, emulsions, essence water, eye creams and introduction liquid, and is simple to prepare, low in cost, convenient to popularize and use and convenient for commercial production, and raw materials are easy to obtain.

Detailed Description

In order to more clearly explain the overall concept of the present application, the following detailed description is given by way of example. In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. It will be apparent, however, to one skilled in the art, that the present invention may be practiced without one or more of these specific details. In other instances, well-known features have not been described in order to avoid obscuring the invention.

The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer.

Wherein the radix Gentianae extract is prepared by Shanghai Gayu Biotech limited company, and is extracted from wild radix Gentianae in Xizang Linzhou county; gamma-aminobutyric acid is provided by Sichuan Tong Cheng amino acid GmbH; the biological sugar gum-1 is provided by Henan Baikang chemical products Co., Ltd, and has a molecular weight of 100-200 ten thousand daltons; the polyglutamic acid sodium is provided by Wuhan Guanghua times biological technology limited company, and the molecular weight is 100-150 kilodaltons; the oat glucan is provided by Xian Zhongying science and technology development limited company, and the molecular weight is 100-200 ten thousand daltons; asiaticoside is provided by Shanghai Fenghui biological science and technology limited; trehalose is supplied by Shanghai-derived Biotech, Inc. and has a molecular weight of 378.33 daltons; urea is supplied by Tech technologies, Inc. of Yinaoka, Beijing, and has a molecular weight of 60.06 daltons; VISIA skin tester is available from Canfield technologies.

In the following embodiments, reagents or instruments used are not indicated by manufacturers, and are all conventional products available by commercial purchase, unless otherwise specified.

EXAMPLE 1 composition ingredient screening

In order to reduce adverse side effects of gamma-aminobutyric acid, 3% of gamma-aminobutyric acid in percentage by mass is compounded with different components, and the balance of water is fully and uniformly mixed at room temperature and then tested.

160 volunteers were searched, and the ages and sexes of the volunteers were randomly determined to meet the standards of the cosmetic hygiene code (2007 edition). And (3) placing 1mL of sample in nasolabial sulcus on two sides of a subject, using a 3% aminobutyric acid aqueous solution as a control group on one side, using gamma-aminobutyric acid compounded with different components on the other side, cleaning after 10-20 minutes, and observing whether a reaction or a delayed reaction occurs within 30 minutes after neutralization. The results are shown in Table 1.

TABLE 1 composition component screening

In conclusion, as can be seen from the data in table 1, in a single compounding experiment, the gamma-aminobutyric acid has a good effect when being compounded with oat glucan, sodium polyglutamate, bioglycan-1, trehalose, urea and asiaticoside, and the pricking feeling is obviously relieved.

Further, oat glucan, sodium polyglutamate, biological carbohydrate gum-1, trehalose, urea and asiaticoside are combined for compound experiments. In the application, the side effect retarding effect of the gamma-aminobutyric acid is obviously retarded when the composition is compounded compared with that of a single component, and particularly, the side effect retarding effect is optimal when all the components are compounded together. Proves that the oat glucan, the sodium polyglutamate, the biological carbohydrate gum-1, the trehalose, the urea and the asiaticoside have good synergistic effect when being compounded with the gamma-aminobutyric acid, and can greatly reduce the pain caused by the gamma-aminobutyric acid.

Composition 1

Putting 3g of gamma-aminobutyric acid, 2g of bioglycan-1, 2g of oat glucan, 4g of sodium polyglutamate, 2g of trehalose, 2g of urea and 84g of deionized water into a beaker, stirring and heating to 60 ℃ until the sodium polyglutamate, the trehalose, the urea and the deionized water are completely dissolved, adding 1g of asiaticoside when the temperature is reduced to 40 ℃, stirring and dissolving, and then cooling to room temperature for later use.

Composition 2

Putting 3g of gamma-aminobutyric acid, 1g of bioglycan-1, 1g of oat glucan, 2g of sodium polyglutamate, 2g of trehalose, 2g of urea and 88g of water into a beaker, stirring and heating to 60 ℃ until the sodium polyglutamate, the trehalose, the urea and the water are completely dissolved, adding 1g of asiaticoside when the temperature is reduced to 40 ℃, and then cooling to room temperature for later use.

Composition 3

Adding 3g of gamma-aminobutyric acid, 1g of bioglycan-1, 2g of oat glucan, 1g of sodium polyglutamate, 2g of trehalose, 2g of urea and 88g of water into a beaker, stirring and heating to 60 ℃ until the sodium polyglutamate, the trehalose, the urea and the water are completely dissolved, adding 1g of asiaticoside when the temperature is reduced to 40 ℃, and then cooling to room temperature for later use.

Composition 4

Adding 3g of gamma-aminobutyric acid, 1g of bioglycan-1, 1g of oat glucan, 2g of sodium polyglutamate, 2g of trehalose, 1g of urea and 89g of water into a beaker, stirring and heating to 60 ℃ until the sodium polyglutamate, the trehalose, the urea and the water are completely dissolved, adding 1g of asiaticoside when the temperature is reduced to 40 ℃, and then cooling to room temperature for later use.

Composition 5

Adding 3g of gamma-aminobutyric acid, 1g of bioglycan-1, 1g of oat glucan, 2g of sodium polyglutamate, 2g of trehalose, 2g of urea and 88.5g of water into a beaker, stirring and heating to 60 ℃ until the sodium polyglutamate, the trehalose, the urea and the water are completely dissolved, adding 0.5g of asiaticoside when the temperature is reduced to 40 ℃, and then cooling to room temperature for later use.

Composition 6

Fully and uniformly mixing 3g of gamma-aminobutyric acid, 1g of bioglycan-1, 1g of oat glucan, 2g of sodium polyglutamate and 88g of water, fully swelling at 60 ℃, adding 2g of trehalose and 2g of urea, stirring until the trehalose and the urea are completely dissolved, cooling to 40 ℃, adding 1g of asiaticoside, and cooling to room temperature for later use.

Control group

3g of gamma-aminobutyric acid and 97g of water are placed in a beaker, stirred and heated to 60 ℃ until completely dissolved, and then cooled to room temperature for later use.

Example 2 sting sensation test

The test was conducted with reference to the method of "evaluation method of cosmetic applicability to facial sensitive skin" study (reference: Maris, Youging, Cheng Ying, etc., research of cosmetic applicability to facial sensitive skin, J.Med.Med.Med.Med. J.Dermatology, 17 th Vol.1, 2018).

And selecting a subject having uncomfortable feeling on the gamma-aminobutyric acid for testing, and testing after screening for one week. The total number of subjects enrolled was 35. 1mL of the sample was placed in the nasolabial sulcus of both sides of the subject, using the comparative example on one side and the example on the other side. The stinging sensation was evaluated separately. The test sites were evaluated for the degree of discomfort of itching, stinging, burning sensation by the subjects, with sting criteria and scores as follows:

no sensation was assigned a score of 0, a score of slightly tingling, a score of 2 with strong tingling, a score of 3 with slight stinging, and a score of 4 with strong stinging.

Sting score ═ (0min +1min +5min comparative flank sting score) - (0min +1min +5min example flank sting score)

The Wilcoxon signed order test method is adopted for statistical analysis, the level is set to be a which is 0.05, the test mean value is in a beneficial direction, and the test result p is less than 0.05, so that the result is valid.

The specific results of the sting scores for compositions 1-6 and the control are shown in Table 2.

TABLE 2 evaluation of stinging sensation at each time point for compositions 1-6 and control

	0min	1min	5min	Sum of
					Composition 1	0.05	0.19	0.37	0.61
Control group	0.32	0.45	0.65	1.42
					Pricking score	0.27	0.26	0.28	0.81
Composition 2	0	0.05	0.1	0.15
					Control group	0.36	0.47	0.75	1.58
Pricking score	0.36	0.42	0.65	1.43
					Composition 3	0	0.12	0.14	0.26
Control group	0.28	0.39	0.65	1.32
					Pricking score	0.28	0.27	0.51	1.06
Composition 4	0.07	0.23	0.19	0.49
					Control group	0.42	0.53	0.68	1.63
Pricking score	0.35	0.3	0.49	1.14
					Composition 5	0.10	0.22	0.45	0.77
Control group	0.31	0.57	0.68	1.56
					Pricking score	0.21	0.35	0.23	0.79
Composition 6	0.04	0.13	0.20	0.37
					Control group	0.55	0.60	0.71	1.86
Pricking score	0.51	0.47	0.51	1.49

As can be seen from the data of compositions 1-3 in Table 2, the effect of suppressing the tingling sensation is enhanced with the increase of the amount of the composition added within a certain compounding ratio. However, too much of the macromolecular humectant component may cause a decrease in the effect of suppressing stinging sensation and may cause burning and itching sensation, and the viscosity increases significantly, making it unsuitable for use in cosmetics.

As can be seen from the comparison of the data of the composition 2 and the composition 4, the inhibition effect of the composition on the stinging sensation is influenced by the different adding proportions of the small molecular humectant, wherein the inhibition effect on the stinging sensation is more obvious when the mass ratio of the urea to the trehalose is 1: 1.

As can be seen from the comparison of the data of the composition 2 and the composition 5, the ratio of the macromolecular humectant, the micromolecular humectant, the soothing agent and the gamma-aminobutyric acid also has an effect on the pain feeling inhibition effect. Wherein when the mass ratio of the gamma-aminobutyric acid, the bioglycan-1, the oat glucan, the sodium polyglutamate, the trehalose, the urea and the asiaticoside is 3:1:1:2:2:2:1, the composition has the best effect of relieving the tingling pain.

Moreover, as can be seen from the comparison of the data of composition 2 and composition 6, the preparation method of the composition also has a certain influence on the effect of the composition. Wherein, the composition prepared by the preparation method of the composition 6 has more obvious effect of inhibiting the side effect brought by the gamma-aminobutyric acid.

In conclusion, the invention compounds the gamma-aminobutyric acid and the composition according to a certain proportion by a specific method, and can effectively relieve the instant discomfort, especially the stabbing pain, brought by the use of the gamma-aminobutyric acid.

Example 3 anti-allergic efficacy test

Respectively and fully and uniformly mixing 5%, 10%, 15% and 20% of gamma-aminobutyric acid, 1g of bioglycan-1, 1g of oat glucan, 2g of sodium polyglutamate and water, fully swelling at 60 ℃, adding 2g of trehalose and 2g of urea, stirring until the trehalose and the urea are fully dissolved, cooling to 40 ℃, adding 1g of asiaticoside, and cooling to room temperature for later use.

The method in example 1 was used for testing, and the stimulation degree of gamma-aminobutyric acid with different content was observed, and the test results are shown in table 3.

TABLE 3 anti-allergic functional test

Content of gamma-aminobutyric acid (%)	Total number of people	Improvements in or relating to	Is not obvious	Weighting device
					5	10	10	-	-
10	10	10	-	-
					15	10	10	-	-
20	10	8	2	-

As can be seen from the data in Table 3, the irritation caused by gamma-aminobutyric acid can be reduced within a certain range by the composition provided by the application, as the skin irritation degree is gradually increased along with the increase of the gamma-aminobutyric acid. Therefore, the composition effectively reduces the irritation of the gamma-aminobutyric acid and improves the safe use content of the gamma-aminobutyric acid in cosmetics, and the content of the gamma-aminobutyric acid in the composition can reach 15 percent at most.

Example 4 wrinkle removal efficacy test

Healthy volunteers with obvious wrinkles on their faces between the ages of 40-60 were selected and randomly divided into two groups of 20 persons, one group using the composition 6 product and the other group using the control group product for 8 weeks.

Before treatment, the face was cleaned and the condition of wrinkles on the skin before treatment was recorded using a VISIA skin detector. The VISIA skin test apparatus recorded the wrinkles after treatment and the test results are shown in table 4.

TABLE 4 comparison of VISIA wrinkle before and after treatment

	Before treatment	After treatment	P value
				Composition 6	24.98±5.72	3.71±2.17	＜0.01
Control group	25.78±2.79	15.79±6.88	＜0.01

As can be seen from the data in Table 4, the wrinkle removing effect of the composition 6 is better than that of a control group, and the composition proves that the biological carbohydrate gum-1, the oat glucan, the sodium polyglutamate, the trehalose, the urea and the asiaticoside in the composition have better synergistic effect, can achieve the effects of layer-by-layer release and multiple wrinkle resistance while effectively relieving the side effect of the gamma-aminobutyric acid, has double synergistic effects of long-acting effect and short-acting effect, and has better wrinkle removing effect.

The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.

Claims

1. The composition for improving stimulation of gamma-aminobutyric acid is characterized by comprising 1-10 parts of gamma-aminobutyric acid, 1-20 parts of macromolecular humectant, 1-20 parts of micromolecular humectant and 0.1-10 parts of soothing agent by mass;

2. The composition according to claim 1, wherein the composition comprises 1-4 parts of gamma-aminobutyric acid, 1-5 parts of macromolecular humectant, 1-5 parts of small molecular humectant and 1-2 parts of soothing agent; preferably, 3 parts of gamma-aminobutyric acid, 4 parts of macromolecular humectant, 4 parts of micromolecular humectant and 1 part of soothing agent.

3. The composition of claim 1, wherein the macromolecular humectant is selected from one or more of biogel-1, oat glucan, sodium polyglutamate, and cellulose sulfate; preferably, the macromolecular humectant comprises bioglycan-1, oat glucan and sodium polyglutamate; more preferably, the mass ratio of the biological sugar gum-1 to the oat glucan to the sodium polyglutamate is (1-5): (1-5): (1-10); more preferably, the mass ratio of the biological sugar gum-1, the oat glucan and the sodium polyglutamate is 1:1: 2.

4. the composition of claim 1, wherein the small molecule humectant is selected from one or more of trehalose, urea, menthol lactate, 4-tert-butylcyclohexanol, water-soluble ceramide, and aloe vera juice; preferably, the micromolecular humectant is trehalose and urea; more preferably, the mass ratio of the trehalose to the urea is (1-10): (1-10); more preferably, the mass ratio of the trehalose to the urea is 1: 1.

5. the composition of claim 1, wherein the soothing agent is selected from one or more of asiaticoside and gentian root extract; more preferably, the soothing agent is asiaticoside.

6. A method of preparing the composition of claim 1, comprising the steps of: mixing and dissolving gamma-aminobutyric acid, a macromolecular humectant, a micromolecular humectant and a soothing agent by using water at the temperature of 40-70 ℃, and uniformly stirring to obtain the composition; preferably, the total amount is 100 parts by mass with water.

7. Use of a composition according to any one of claims 1 to 5 or obtained by the preparation method according to claim 6 for anti-wrinkle, whitening and/or moisturizing; preferably, the composition is for use in anti-wrinkle.

8. A cosmetic product comprising a composition according to any one of claims 1 to 5 or a composition prepared by a process according to claim 6.

9. The cosmetic according to claim 8, wherein the content of gamma-aminobutyric acid in the cosmetic is 0.1 to 15% by mass, preferably 0.5 to 15% by mass, more preferably 5.1 to 15% by mass.

10. The cosmetic according to claim 8, wherein the cosmetic is a mask, a cream, a lotion, a serum, a lotion, an eye cream and/or an introduction solution.