CN114303879A - Application of waste argil as biological fertilizer, compound biological fertilizer and application thereof - Google Patents
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Abstract
The invention relates to an application of waste argil as a biological fertilizer, a compound biological fertilizer and an application thereof, which comprises the steps of mixing the waste argil with a culture soil matrix to prepare culture soil, adding microalgae biomass into the culture soil before transplanting plant seedlings into the culture soil or during planting in the culture soil or after planting in the culture soil, wherein the feeding mass ratio of the waste argil to the culture soil matrix is 1: (5-155). The method improves the stress effect of the waste argil on plants, shortens the growth period of the plants and realizes the reutilization of the waste argil by the compound use of the waste argil and the microalgae biomass.
Description
Technical Field
The invention relates to application of waste argil as a biological fertilizer, a compound biological fertilizer and application thereof.
Background
The argil is grey white granular powder with special adsorption capacity, large specific surface area and pore volume, and special adsorption capacity and ion exchange performance. Therefore, the activated clay is widely applied to the decoloring and refining of mineral oil, animal and vegetable oil, wax and organic liquid. After the clay is applied to the oil product decolorization treatment, the clay no longer has adsorption decolorization capacity, which results in the generation of a large amount of waste clay. The waste argil from different sources is always attached to different components, and improper treatment and recovery not only can cause resource waste, but also can bring great harm to the environment. The waste clay has different attached components, so the recovery treatment is very difficult, the recovery and utilization cost is high, and the value is low. At present, the waste clay is mainly treated by burning or burying, but with the increasing requirements of environmental protection related laws and regulations in recent years, the method is no longer acceptable.
Therefore, how to recycle the waste clay becomes a technical problem which needs to be solved urgently.
Disclosure of Invention
The invention aims to provide application of waste argil as a biological fertilizer, a compound biological fertilizer and application thereof, solves the problems of high recycling cost and low value of the waste argil in the prior art, and realizes resource utilization of the waste argil.
In order to achieve the purpose, the invention adopts the technical scheme that:
the application of waste clay as a biological fertilizer is characterized in that the waste clay is mixed with a culture soil matrix to prepare culture soil, microalgae biomass is added into the culture soil before plant seedlings are transplanted to the culture soil or when the plant seedlings are planted to the culture soil or after the plant seedlings are planted to the culture soil, and the feeding mass ratio of the waste clay to the culture soil matrix is 1: (5-155).
Preferably, the feeding mass ratio of the waste argil to the culture soil matrix is 1: (10-20).
Further preferably, the feeding mass ratio of the waste argil to the culture soil matrix is 1: (15-20).
Preferably, the microalgae biomass comprises one or more of a suspension of microalgae cells, a microalgal flour, an aqueous solution of microalgal flour, and the microalgae in the microalgae biomass comprises chlorella pyrenoidosa.
Preferably, the microbial density OD of the microalgae cell suspension600＝0.5～0.8。
Further preferably, the microbial density OD of the microalgae cell suspension600＝0.6～0.7。
Preferably, the mass concentration of the water solution of the microalgal flour is 0.1-10 g/L.
More preferably, the mass concentration of the aqueous solution of the microalgal flour is 0.5-10 g/L.
Preferably, the feeding mass ratio of the waste argil to the microalgae powder is 1: (0.005-1).
Further preferably, the feeding mass ratio of the waste argil to the microalgae powder is 1: (0.1-0.5).
According to some preferred embodiments, the microalgal biomass is added to the culture soil in a liquid root drench.
According to further preferred embodiments, the microalgal biomass is added to the culture soil in the form of a landfill.
Preferably, the oil content of the spent bleaching earth is greater than or equal to 20%.
More preferably, the oil content of the waste argil is 35-50%.
Preferably, the pH value of the waste argil is 2.5-5.5.
More preferably, the pH value of the waste argil is 3-4.
Preferably, the culture soil matrix comprises one or more of peat, vermiculite, perlite, soil.
Further preferably, the culture soil matrix comprises the peat, the vermiculite and the perlite, and the feeding mass ratio of the peat, the vermiculite and the perlite is (10-20): (10-20): 1.
still further preferably, the feeding mass ratio of the peat, the vermiculite and the perlite is (13-18): (13-16): 1.
preferably, the plant comprises one or more of grain, fruit and vegetable, flower and grass.
The second aspect of the invention provides a compound biological fertilizer, which comprises waste argil and microalgae biomass, wherein the feeding mass ratio of the waste argil to the microalgae biomass is 1: (0.01-1), wherein the waste clay comprises oil-containing waste clay, and the oil content of the waste clay is greater than or equal to 20%.
Preferably, the oil content of the waste argil is 35-50%.
Preferably, the pH value of the waste argil is 2.5-5.5.
More preferably, the pH value of the waste argil is 3-4.
Preferably, the compound biological fertilizer also comprises a culture soil matrix.
Further preferably, the feeding mass ratio of the culture soil matrix to the waste argil is (10-20): 1.
more preferably, the feeding mass ratio of the culture soil matrix to the waste argil is (13-18): 1.
preferably, the microalgae biomass comprises one or more of a suspension of microalgae cells, a microalgal flour, an aqueous solution of microalgal flour, and the microalgae in the microalgae biomass comprises chlorella pyrenoidosa.
The third aspect of the invention provides a preparation method of the compound biological fertilizer, which comprises the step of uniformly mixing the waste argil and the microalgae biomass to prepare the compound biological fertilizer.
A fourth aspect of the invention provides a use of the compound biological fertilizer, which comprises burying the compound biological fertilizer in soil.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages:
the method improves the stress effect of the waste argil on plants, shortens the growth period of the plants and realizes the reutilization of the waste argil by the compound use of the waste argil and the microalgae biomass.
Drawings
FIG. 1 is a graph showing the effect of various forms of algal manure used in combination with spent bleaching earth on the growth of Arabidopsis in an embodiment of the present invention;
FIG. 2 is a graph showing the growth of Arabidopsis thaliana in the case where raw Clay (Clay) or spent Clay (SBC) is directly used as a culture soil substrate in the comparative example of the present invention;
FIG. 3 shows the growth of Arabidopsis thaliana with different mixing methods of spent bleaching earth and algae powder in the examples and comparative examples of the present invention.
Detailed Description
The waste clay has different adhering components, so that the recovery and treatment are difficult. At present, the waste clay is mainly treated by burning or burying, and the like, and no matter the waste clay is treated by burning or burying, the environment is polluted and resources are wasted.
Based on the defects of the prior art, the applicant obtains the scheme of the application through long-term experiments and a large amount of research, and further elaborates on the scheme.
The application of waste argil as biological fertilizer includes mixing waste argil with culture soil matrix to prepare culture soil, and adding microalgae biomass into the culture soil before, during or after transplanting the plant seedling into the culture soil.
The algae has nitrogen fixing property, so that the algae can be applied to crops such as grains, fruits and vegetables, flowers and lawns as fertilizer to achieve the purposes of promoting seed germination, improving yield and improving quality and soil quality. Research shows that the seaweed residue organic fertilizer has obvious promotion effect on the germination and growth of the pakchoi, can be popularized and used, and is particularly suitable for being applied to saline-alkali soil. The fertilization of different microalgae has a promoting effect on the growth of potted cucumbers, which indicates that the soil fertility can be improved to a certain degree by applying the microalgae, and also indicates that the carbon-fixing microalgae can be used for improving the carbon content of soil, so that the fixed carbon is converted into the carbon required by plant growth. The chlorella extract can improve the germination rate, the germination vigor and the germination index of the seeds of Chinese cabbage, leaf mustard and radish in a certain treatment concentration range, end the germination period in advance, promote the elongation of radicles in the seed germination process and comprehensively improve the seed germination condition. The chlorella which is recycled from the waste water has a certain promotion effect on the growth of arabidopsis thaliana as a biological algae liquid fertilizer, and nutrient-rich elements which are absorbed and fixed from the waste water are released into soil in a fertilizer form and are used for the growth and utilization of plants under the action of soil microorganisms. The organic matter in the waste clay is about 50 percent, if a corresponding formula is developed, some elements are added, and the waste clay is a very good biological organic fertilizer and organic compound fertilizer. The method improves the stress effect of the waste argil on plants, shortens the growth period of the plants and realizes the reutilization of the waste argil by combining the waste argil and the microalgae biomass.
The above-mentioned "mixing" includes mixing the waste clay with the culture soil base and stirring them uniformly, or burying the waste clay in the culture soil base.
In the present invention, the waste clay includes oil-containing waste clay. Specifically, the waste clay includes clay used for mineral oil, animal and vegetable oil and fat, wax production and the waste clay generated by the decoloration and purification of organic liquid. The oil content (mass) in the spent bleaching earth is 20% or more, and preferably 35 to 50%, for example, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, and the like. The pH value of the waste clay is 2.5-5.5, such as 2.5, 3, 3.5, 4, 4.5, 5, 5.5, etc.
In the invention, the culture soil matrix comprises one or more of peat, vermiculite, perlite and soil. According to some preferred embodiments, the culture soil matrix comprises peat, vermiculite and perlite, and the feed mass ratio of the peat, the vermiculite and the perlite is (10-20): (10-20): 1, e.g. 10: 10: 1. 15: 10: 1. 20: 10: 1. 10: 15: 1. 10: 20: 1. 15: 15: 1. 20: 20: 1, etc.
In the invention, the feeding mass ratio of the waste argil to the culture soil matrix is 1: (5-155). The waste argil contains a large amount of grease, can be attached to the roots of plants, cannot be absorbed by the roots, is also favorable for air circulation, and hinders the respiration and substance exchange of the roots. In addition, various heavy metal ions with high concentration or toxic compounds may remain in the waste argil, which can inhibit the growth of plants; the total concentration of inorganic salt ions in the waste argil is high, the effect of 'overfertilization' is shown, and the plant growth is also influenced. Therefore, the amount of the spent clay added is not so large, and preferably, the mass ratio of the spent clay to the culture soil matrix is 1: (10-20), for example, the ratio of 1: 10. 1: 11. 1: 12. 1: 13. 1: 14. 1: 15. 1: 16. 1: 17. 1: 18. 1: 19. 1: 20, etc.
The plant in the invention includes but is not limited to one or more of plants such as grains, fruits and vegetables, flowers, grass and the like.
In the invention, the microalgae biomass comprises one or more of microalgae cell suspension, microalgae powder and a microalgae powder aqueous solution, and the microalgae in the microalgae biomass comprises chlorella pyrenoidosa. The microbial density OD600 of the microalgae cell suspension is 0.5 to 0.8, and may be 0.5, 0.6, 0.7, 0.8, or the like. The mass concentration of the aqueous solution of the microalgal flour is 0.1-10 g/L, for example, 0.1g/L, 1g/L, 2g/L, 3g/L, 4g/L, 5g/L, 6g/L, 7g/L, 8g/L, 9g/L, 10g/L, etc. When the microalgae biomass and the waste argil are used together, the addition amount of the microalgae biomass is too low, and a good synergistic effect cannot be achieved; when the dosage reaches a higher dosage, the stress effect of the waste argil can be improved, even the stress of the waste argil can be eliminated, and the effect of promoting growth is achieved; when the dosage of the microalgae biomass is too high, the microalgae biomass becomes a 'hotbed' for breeding the mould, and the cultured soil is easy to mildew. Further, the feeding mass ratio of the waste argil to the microalgae powder is 1: (0.005-1), for example, 1: 0.05, 1: 0.1, 1: 0.2, 1: 0.3, 1: 0.4, 1: 0.5, etc.
Furthermore, the microalgae biomass is a microalgae cell suspension or a microalgae powder aqueous solution, and the microalgae biomass can enter culture soil in a root irrigation mode. When the microalgae biomass is microalgae powder, the microalgae powder can be added into the culture soil in a landfill form, for example, the microalgae powder can be uniformly mixed with the waste argil and then buried, and the culture soil matrix can be added and uniformly mixed and then buried. The thickness of the filling layer is not less than 3-5 cm, and the filling layer is filled with culture soil matrix after being uniformly mixed, so that a better slow-release effect is achieved.
A compound fertilizer biological fertilizer comprises waste argil and microalgae biomass.
The feeding mass ratio of the waste argil to the microalgae biomass is 1: (0.01 to 1), for example, 1: 0.01, 1: 0.05, 1: 0.1, 1: 0.2, 1: 0.3, 1: 0.4, 1: 0.5, 1: 0.6, 1: 0.7, 1: 0.8, 1: 0.9, 1: 1, etc. The spent bleaching earth and the microalgae biomass are as described above, and are not described herein in detail.
The present invention will be further described with reference to the following examples. However, the present invention is not limited to the following examples. The implementation conditions adopted in the embodiments can be further adjusted according to different requirements of specific use, and the implementation conditions not mentioned are conventional conditions in the industry. The technical features of the embodiments of the present invention may be combined with each other as long as they do not conflict with each other.
The experimental materials in the following examples and comparative examples are as follows:
(1) the test plant species: providing a first batch of seeds by a research group of plant hormones and reproductive development of arabidopsis thaliana Columbia type Shanghai university of transportation;
(2) plant growth culture soil matrix: peat, vermiculite and perlite (volume ratio) is 15:14: 1;
(3) plant growth environmental conditions: the temperature is 22-23 ℃ of room temperature under the control of an air conditioner, the humidity is 60-80%, and the illumination is 7000+ lux;
(4) specification of the flowerpot: 10x10x8.5 cm, the soil loading is about 400cm3，75～80g；
(5) Oily spent bleaching earth (SBC): waste clay generated after the decolorization of industrial oil, provided by Baoma grease equipment Limited company in Taicang city;
(6) commercial fertilizer control group fertilizer: no. 1 water-soluble fertilizer with more flowers and light blue crystals; the microalgae is fresh water chlorella pyrenoidosa;
(7) arabidopsis seed preservation conditions: mixing with silica gel desiccant, placing in a 2mL centrifuge tube, mixing with silica gel desiccant, placing in a 50mL centrifuge tube, and storing in a dry box;
(8) culture medium for germination of arabidopsis seed: germinating directly in the culture soil matrix in the above (2).
The basic physicochemical properties of the oil-containing spent bleaching clay are shown in table 1 below, and the spent bleaching clay in the following examples and comparative examples refers to the mass ratio of the blocky spent bleaching clay to the powdery spent bleaching clay of 1: 1.
TABLE 1
The method for measuring pH in Table 1 is described in "Standard of agricultural industry of the people's republic of China," determination of pH in soil (NY/T1377-2007) "; the method for measuring the oil content and the water content is as follows.
The method for measuring the oil content is briefly described as follows:
uniformly mixing waste argil samples, crushing massive and large-particle soil samples, paving dozens of grams of fine-particle samples into thin layers in an open container, and drying for more than 24 hours in an oven at the temperature of 40-55 ℃; weighing as soon as possible after drying, and subpackaging into 0.1-0.5 g (m)0) Placing the mixture into a 2mL or 15mL centrifugal tube, and adding a steel ball (the steel ball with the diameter of 3-5mm for the common experiment); extracting with a chloroform-methanol organic mixed solvent (v/v ═ 2: 1), wherein the solvent is not more than 2mL in a 2mL centrifuge tube every time, and 3-5 mL in a 15mL centrifuge tube every time; oscillating and crushing for 4-6 min, and centrifuging at 4 ℃ (6000-10000 rpm, 6-10 min/time); extracting for 3-5 times until the supernatant becomes colorless and transparent after centrifugation; all supernatants of each sample were collected separately and dried at 60 ℃ for more than 24 hours and weighed (m)1) And (3) putting the organic solvent into a 15 or 50mL centrifugal tube, carrying out water bath at 50-65 ℃ in a fume hood for more than 12 hours until the organic solvent is completely volatilized and the volume is unchanged, and transferring the organic solvent to a 50-60 ℃ oven to dry the organic solvent to constant weight (more than 72 hours). Weighing the total mass (m) of grease at the bottom of the centrifuge tube and the tube after drying2)。
Oil content ═ m2-m1)/m0*100％。
The method for measuring the water content is briefly described as follows:
waste white paper provided by companies or manufacturersStoring the soil sample in a cool and dry room, taking out tens of grams of sample, crushing the sample into fine particles by using a glass rod, and transferring the fine particles into a 15-50 mL centrifugal tube (m) after drying and weighing0) Weigh centrifuge tube and sample mass (m)1) (ii) a Drying the open centrifugal tube in an oven at 40-55 ℃ for more than 48 hours, quickly sampling and weighing m2(multiple sampling can reduce errors).
Water content ═ m1-m2)/(m1-m0)*100％。
Example 1
5g of waste oil-containing carclazyte (SBC) and 77.5g of culture soil matrix are uniformly mixed to prepare culture soil, and the culture soil is potted. After being wetted from bottom to top of the flowerpot by tap water, the healthy arabidopsis seedlings which germinate for more than two weeks are transplanted to the surface of the culture soil and grow under the plant growth environment condition of the step (3).
Root irrigation with algal cell suspension (algal cell suspension OD) on the day of seedling transplantation6000.510), after which the roots are drenched with algae cell suspension at intervals of 8 days for a total of three drenches, each time with 20ml of algae cell suspension.
Example 2
The difference from example 1 is that the algae cell suspension is an algae cell suspension that has been subjected to sonication. The ultrasonic conditions were: working time is 6.0s, intermittent operation is 4.0s, total ultrasonic time is 30min, and OD after ultrasonic treatment600＝0.600～0.700。
Example 3
The difference from example 1 is that the root irrigation was carried out using 0.5g/L algae meal solution.
The effect of the different forms of algal manures of examples 1-3 used in combination with spent bleaching earth on the growth of Arabidopsis is shown in FIG. 1. Wherein, the algae cell suspension + SBC (a), the ultrasonic treatment algae cell suspension + SBC (b), the algae powder solution + SBC (c) and SBC (d). As can be seen from FIG. 1, the combination of the different forms of the algae fertilizer and the waste clay can promote the growth of the Arabidopsis, and the combination effect of the algae powder solution and the waste clay is better.
Comparative example 1
The original argil (Clay) and the oil-containing waste argil are respectively directly used as the matrix for growing the arabidopsis thaliana, and after the arabidopsis thaliana is wetted by tap water, healthy arabidopsis thaliana seedlings are transplanted onto the matrix. Observation shows that compared with a general culture soil matrix (peat-vermiculite-perlite, 15:14:1), the original argil and the oil-containing waste argil can keep a moist and soft state for a long time after absorbing water, the water-retaining property is extremely strong, and even oily liquid is leached from the bottom of the oil-containing waste argil. Arabidopsis seedlings could not develop on both substrates and died within 3-6 days. The results are shown in FIG. 2.
Comparative example 2
The difference from example 1 is that the culture soil is a culture soil matrix, the root is not irrigated with algae cell suspension during the growth period of the arabidopsis seedlings, and tap water is only irrigated for the later management.
Comparative example 3
The difference from example 1 is that the culture soil is a culture soil matrix.
Comparative example 4
The difference from example 1 is that the root was not drenched with algae suspension during the growth of Arabidopsis seedlings, and much flowers at a concentration of 1g/L were used for fertilization.
The growth of Arabidopsis in examples 1-2 and comparative examples 2-3 is shown in Table 2 below.
TABLE 2
Neither native argil nor oily waste argil can be used as a culture soil matrix for growth of arabidopsis thaliana, and both are easy to absorb water, and have strong water retention after water absorption and wetting, so that root rot of plants can be caused, respiration and substance exchange of plant roots are not facilitated, and finally the plants cannot grow. The waste argil mixed into the culture soil substrate can also influence the growth and development of arabidopsis thaliana, and the main reasons are as follows: 1) the waste carclazyte contains a plurality of oil components, is attached to the root of arabidopsis thaliana, can not be absorbed by the root, changes the soil structure, is not beneficial to air circulation, and blocks the respiration and the material exchange of the root; 2) various heavy metal ions with high concentration or toxic compounds may be left in the waste carclazyte, so that the growth of arabidopsis is inhibited; 3) the total concentration of inorganic salt ions in the waste argil is high, and the effect of 'overfertilization' is shown.
When the algae suspension (the chlorella pyrenoidosa) and the waste argil are used together, the growth of arabidopsis is obviously promoted, and the potential of shortening the plant growth cycle is realized.
Example 4
77.5g of culture soil matrix is potted, and a mixture of 0.5g of algae powder and 5g of waste oil-containing clay is filled in the middle of the pot culture. Transplanting healthy Arabidopsis seedlings which germinate for more than two weeks to the surface of culture soil, and growing under the plant growth environment condition of the above (3).
Example 5
The difference from example 4 is that: the content of algae powder is different, and the content of algae powder in the embodiment is 1 g.
Example 6
The difference from example 4 is that: the content of algae powder is different, and the content of algae powder in the embodiment is 1.5 g.
Comparative example 5
The difference from example 4 is that: the mixture of the algae powder and the oil-containing spent bleaching clay is evenly mixed with the culture soil matrix.
Comparative example 6
The difference from example 5 is that: the mixture of the algae powder and the oil-containing spent bleaching clay is evenly mixed with the culture soil matrix.
Comparative example 7
The difference from example 6 is that: the mixture of the algae powder and the oil-containing spent bleaching clay is evenly mixed with the culture soil matrix.
The results of the experiments of examples 4 to 6 and comparative examples 5 to 7 are shown in FIG. 3, where 4d and 47d in FIG. 3 represent the growth of the plants after 4 th and 47 th days after the transplantation of seedlings, respectively; the table in fig. 3 shows the fertilizer application situation of each potted plant in the diagram, SBC shows the control containing 5.0g of waste clay, PT shows the blank control without any additive, and the additive amount of the waste clay of all experimental groups is 5.0 g; figure a is a photograph taken while mixed in a landfill.
The larger the addition amount of the algae powder is, the more likely the mold breeding on the surface of the culture soil is. The combination of the oil-containing spent bleaching clay and the algae powder can inhibit the growth of the mold to a certain extent. When the mixture of the algae powder and the waste argil is buried in the middle of a pot, the survival rate of seedlings in the middle and later stages is high, and when the mixture of the algae powder and the waste argil and culture soil are uniformly stirred for use, the seedlings in the middle and later stages are all dead and have a mildew growing phenomenon.
The present invention has been described in detail in order to enable those skilled in the art to understand the invention and to practice it, and it is not intended to limit the scope of the invention, and all equivalent changes and modifications made according to the spirit of the present invention should be covered by the present invention.
Claims (10)
1. The application of the waste argil as a biological fertilizer is characterized in that: mixing waste clay and a culture soil matrix to prepare culture soil, and adding microalgae biomass into the culture soil before transplanting plant seedlings into the culture soil or when planting the plant seedlings into the culture soil or after planting the plant seedlings into the culture soil, wherein the feeding mass ratio of the waste clay to the culture soil matrix is 1: (5-155).
2. The use of spent bleaching earth as a biofertilizer according to claim 1, characterized in that: the microalgae biomass comprises one or more of microalgae cell suspension, microalgae powder and a microalgae powder aqueous solution, and the microalgae in the microalgae biomass comprises chlorella pyrenoidosa.
3. The use of spent bleaching earth as a biofertilizer according to claim 2, characterized in that: the microbial density OD of the microalgae cell suspension600=0.5~0.8；
And/or the mass concentration of the water solution of the microalgal flour is 0.1-10 g/L;
and/or the mass ratio of the waste argil to the microalgae powder is 1: (0.005-1).
4. The use of spent bleaching earth as a biofertilizer according to claim 1, characterized in that: the microalgae biomass is added to the culture soil in a liquid root irrigation manner, and/or the microalgae biomass is added to the culture soil in a landfill manner.
5. The use of spent bleaching earth as a biofertilizer according to claim 1, characterized in that: the oil content of the waste argil is more than or equal to 20%, and/or the pH value of the waste argil is 2.5-5.5.
6. The use of spent bleaching earth as a biofertilizer according to claim 1, characterized in that: the culture soil matrix comprises one or more of peat, vermiculite, perlite and soil.
7. The use of spent bleaching earth as a biofertilizer according to claim 6, characterized in that: the culture soil matrix comprises the peat, the vermiculite and the perlite, and the feeding mass ratio of the peat to the vermiculite to the perlite is (10-20): (10-20): 1.
8. a compound biological fertilizer is characterized in that: the compound biological fertilizer comprises waste argil and microalgae biomass, wherein the mass ratio of the waste argil to the microalgae biomass is 1: (0.01-1), wherein the waste clay comprises oil-containing waste clay, and the oil content of the waste clay is greater than or equal to 20%.
9. The compound biofertilizer of claim 8, characterized in that: the oil content of the waste argil is 35-50%;
and/or the pH value of the waste argil is 2.5-5.5;
and/or the microalgae biomass comprises one or more of microalgae cell suspension, microalgae powder and a microalgae powder aqueous solution, and the microalgae in the microalgae biomass comprises chlorella pyrenoidosa.
10. Use of a composite bio-fertilizer according to claim 8 or 9, characterized in that: and burying the compound biological fertilizer in soil.
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