CN114286864B - 慢病毒载体产生方法及构建体 - Google Patents

慢病毒载体产生方法及构建体

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Publication number
CN114286864B
CN114286864B CN202080059158.XA CN202080059158A CN114286864B CN 114286864 B CN114286864 B CN 114286864B CN 202080059158 A CN202080059158 A CN 202080059158A CN 114286864 B CN114286864 B CN 114286864B
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gene
lentiviral
promoter
vector
transposon
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CN114286864A (zh
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Y·申
A·赛斯
顾炳南
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Lonza Walkersville Inc
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Lonza Walkersville Inc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155—Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16—HIV-1 ; HIV-2
    • C07K14/161—HIV-1 ; HIV-2 gag-pol, e.g. p55, p24/25, p17/18, p7, p6, p66/68, p51/52, p31/34, p32, p40
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    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155—Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16—HIV-1 ; HIV-2
    • C07K14/162—HIV-1 ; HIV-2 env, e.g. gp160, gp110/120, gp41, V3, peptid T, CD4-Binding site
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    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155—Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16—HIV-1 ; HIV-2
    • C07K14/163—Regulatory proteins, e.g. tat, nef, rev, vif, vpu, vpr, vpt, vpx
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material
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    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material
    • C12N2740/16052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20211—Vesiculovirus, e.g. vesicular stomatitis Indiana virus
    • C12N2760/20222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
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  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • AIDS & HIV (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN202080059158.XA 2019-08-23 2020-08-24 慢病毒载体产生方法及构建体 Active CN114286864B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962890904P 2019-08-23 2019-08-23
US62/890,904 2019-08-23
PCT/US2020/047650 WO2021041322A1 (en) 2019-08-23 2020-08-24 Methods and constructs for production of lentiviral vector

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CN114286864A CN114286864A (zh) 2022-04-05
CN114286864B true CN114286864B (zh) 2025-08-15

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US (3) US12037599B2 (https=)
EP (1) EP4017987A4 (https=)
JP (2) JP2022551219A (https=)
KR (1) KR20220047635A (https=)
CN (1) CN114286864B (https=)
IL (1) IL290820A (https=)
WO (1) WO2021041322A1 (https=)

Families Citing this family (4)

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Publication number Priority date Publication date Assignee Title
WO2022192193A1 (en) * 2021-03-09 2022-09-15 The Board Of Regents Of The University Of Texas System Methods and compositions for mutagenesis screening in mammalian cells
CN117529491A (zh) * 2021-04-23 2024-02-06 阿西莫夫公司 用于慢病毒载体产生的稳定产生系统
WO2023083760A1 (en) 2021-11-09 2023-05-19 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Koala retrovirus envelope glycoproteins and uses thereof
KR20260013198A (ko) * 2023-05-12 2026-01-27 론자 바이올로직스 피엘씨 고수율 생성물 생산을 위한 트랜스포사제를 이용한 하이브리드 일시적 형질감염

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017144893A1 (en) * 2016-02-26 2017-08-31 Ucl Business Plc Retrovirus packaging cell

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7262049B2 (en) * 1999-03-16 2007-08-28 Dana-Farber Cancer Institute, Inc. Pseudotyped lentiviral vectors and uses thereof
US10087463B2 (en) 2000-10-31 2018-10-02 University Of Notre Dame Du Lac Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector piggyBac
ATE437221T1 (de) 2001-05-14 2009-08-15 Gbp Ip Llc Lentivirale vektoren kodierend für gerinnungsfaktoren für die gentherapie
US20090042297A1 (en) * 2007-06-01 2009-02-12 George Jr Alfred L Piggybac transposon-based vectors and methods of nucleic acid integration
DK2307551T3 (en) 2008-06-18 2017-03-20 Oxford Biomedica (Uk) Ltd CLEANING RETROVIRAL VECTORS
WO2011097447A2 (en) 2010-02-04 2011-08-11 Neurologix, Inc. Production of recombinant virus
SI2480678T1 (sl) 2010-09-02 2014-06-30 Molmed Spa Stabilno proizvajanje lentivirusnih vektorjev
CA3108569A1 (en) 2010-10-05 2012-04-12 Bayer Cropscience Lp A system and method of establishing an agricultural pedigree for at least one agricultural product
US20140315294A1 (en) 2011-11-24 2014-10-23 Genethon Scalable lentiviral vector production system compatible with industrial pharmaceutical applications
WO2013090409A1 (en) 2011-12-12 2013-06-20 The Children's Hospital Of Philadelphia Large commercial scale lentiviral vector production system and vectors produced thereby
EP2970920B1 (en) 2013-03-15 2018-04-25 The Children's Hospital of Philadelphia Scalable manufacturing process to produce recombinant lentiviral vectors in serum-free suspension cell culture system
FR3010720B1 (fr) 2013-09-16 2017-08-11 Genethon Procede de production de virus enveloppes
FR3014901B1 (fr) 2013-12-17 2017-06-09 Genethon Procede de purification de virus ou vecteurs viraux enveloppes
GB201417042D0 (en) 2014-09-29 2014-11-12 Fkd Therapies Oy Method
US20160267432A1 (en) 2015-03-13 2016-09-15 GeoPRI, LLC Systems and methods for tracking an individual unit
US20160350715A1 (en) 2015-05-29 2016-12-01 Eugenio Minvielle Nutrition Based Food System and Method
JP7098521B2 (ja) * 2015-11-24 2022-07-11 グラクソスミスクライン、インテレクチュアル、プロパティー、ディベロップメント、リミテッド レトロウイルス産生のための安定な細胞株
GB201603372D0 (en) * 2016-02-26 2016-04-13 Ucl Business Plc Cell
US10699284B2 (en) 2016-08-10 2020-06-30 Label Insight, Inc. Information management system for product ingredients to fill gaps in the market and to determine proper attribution
GB201705927D0 (en) 2017-04-12 2017-05-24 Oxford Genetics Ltd Vector
GB201715052D0 (en) 2017-09-19 2017-11-01 Oxford Genetics Ltd Vectors
EP3456822A1 (en) 2017-09-19 2019-03-20 CEVEC Pharmaceuticals GmbH Inducible aav rep genes
KR20210108406A (ko) 2018-12-21 2021-09-02 론자 워커스빌 아이엔씨. 바이러스 벡터의 자동화된 생산

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017144893A1 (en) * 2016-02-26 2017-08-31 Ucl Business Plc Retrovirus packaging cell

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Lentiviral vector packaging cell line development using genome editing to target optimal loci discovered by high- throughput DNA barcoding;Alberto Molina Gil;A thesis submitted for the degree of Doctor of Philosophy 2017;20170101;全文 *

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IL290820A (en) 2022-04-01
US20240336936A1 (en) 2024-10-10
WO2021041322A1 (en) 2021-03-04
US12037599B2 (en) 2024-07-16
EP4017987A4 (en) 2023-10-04
CN114286864A (zh) 2022-04-05
EP4017987A1 (en) 2022-06-29
JP2022551219A (ja) 2022-12-08
US20250263749A1 (en) 2025-08-21
US20210062220A1 (en) 2021-03-04
JP2025148329A (ja) 2025-10-07
KR20220047635A (ko) 2022-04-18
US12391960B2 (en) 2025-08-19

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