CN114190223A - Fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae - Google Patents

Abstract

The invention discloses a fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae, which is characterized in that during the planting process of fruit trees, a root mixed nutrient solution is applied to the roots of the fruit trees, and a foliar nutrient solution is applied to the foliage of the fruit trees, wherein the components of the root mixed nutrient solution comprise the nitrogen-fixing blue algae and the photosynthetic nutrient green algae, and the components of the foliar nutrient solution comprise the photosynthetic nutrient green algae. The fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae provided by the invention can effectively reduce pesticide residues in fruits, increase the sugar degree and the polyunsaturated fatty acid content of the fruits, improve the taste of the fruits, simultaneously ensure that the fruits are more green and healthy to eat, and effectively improve the income of fruit growers.

Description

Fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae

Technical Field

The invention belongs to the technical field of fruit tree planting, and particularly relates to a fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae.

Background

During the period of planting fruit trees, the application of large amounts of chemical inputs has been a routine practice for fruit growers in order to make the fruit trees grow healthily and to increase the fruit tree yield. The fruit flavor is easy to change, the taste is influenced, a large amount of pesticide residues exist in the fruits, the pesticide has the characteristics of high toxicity, high residue, difficult degradation and the like, the health of a human body is influenced when the fruits enter the human body, and poisoning and death of the human body even can be caused in severe cases. Therefore, for fruit tree planting, it is urgently needed to provide a novel cultivation technology, which can improve the content of nutrient substances and taste of fruits and reduce pesticide residues in the fruits.

Disclosure of Invention

The invention aims to: provides a fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae, which can effectively reduce pesticide residue in fruits and improve fruit taste.

The technical scheme adopted by the invention is as follows:

a fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae is characterized in that during the planting process of fruit trees, a root mixed nutrient solution is applied to the roots of the fruit trees, and a foliar nutrient solution is applied to the foliar surfaces of the fruit trees, wherein the components of the root mixed nutrient solution comprise the nitrogen-fixing blue algae and the photosynthetic nutrient green algae, and the components of the foliar nutrient solution comprise the photosynthetic nutrient green algae.

Still further, the root mixed nutrient solution is applied in the dormancy stage and/or the growth stage of the new branches of the fruit tree, and the foliar nutrient solution is applied in the growth stage of the new branches of the fruit tree and/or the slow growth stage of the fruits.

Furthermore, the root mixed nutrient solution comprises a nitrogen fixing blue algae solution and a photosynthetic nutrient green algae solution, wherein the number of nitrogen fixing blue algae in the nitrogen fixing blue algae solution is more than or equal to 500 ten thousand per mL, and the number of photosynthetic nutrient green algae in the photosynthetic nutrient green algae solution is more than or equal to 500 ten thousand per mL.

Furthermore, the nitrogen-fixing blue algae is anabaena azotoformans, and the photosynthetic nutrient green algae is chlorella pyrenoidosa.

Furthermore, water is added into the root mixed nutrient solution and the leaf surface nutrient solution for dilution.

Furthermore, the nitrogen-fixing blue algae solution and the photosynthetic nutrient green algae solution are obtained by culturing nitrogen-fixing blue algae and photosynthetic nutrient green algae in a culture medium, wherein the culture medium comprises water and the following components in concentration: 11-15mg/L C₁₀H₁₆N₂O₈、11-15mg/L C₆H₈O₇、0.11-0.15mg/L FeSO₄·7H₂O、0.11-0.15g/L NaHCO₃、0.11-0.15g/L CaCl₂、0.11-0.15g/L MgSO₄·7H₂O、0.55-0.75g/LK₂HPO₄、0.11-0.15g/L KH₂PO₄、11-15mg/L MnCl₂·4H₂O、0.11-0.15mg/L ZnSO₄·7H₂O、0.11-0.15mg/L CuSO₄·5H₂O、0.11-0.15mg/L Na₂MoO₄·2H₂O、11-15mg/L H₃BO₃11-15mg/L pinellia agglutinin protein.

Furthermore, the pH value of the water used in the culture medium is 7.5-8.5, the TDS value is 19-21, and when the nitrogen-fixing blue algae/photosynthetic nutrient green algae are put into the culture medium for culture, the illumination is continued and purified air is introduced, the pressure of the purified air is 0.5-0.7MPa, the illumination intensity is 2500-.

Furthermore, in the fruit tree planting process, the production management of the fruit trees in each growth cycle comprises the following modes:

and (4) nutritional reserve stage: cultivating and fertilizing;

a dormant period: applying root mixed nutrient solution to the roots of the fruit trees, wherein the application amount is 200-400mL per mu in terms of the protein nucleus chlorella solution;

the growth period of the new branch is as follows: disease prevention, fertilization, and application of a root mixed nutrient solution and a leaf surface nutrient solution, wherein the application amount of the root mixed nutrient solution and the leaf surface nutrient solution is 400mL per mu in terms of chlorella pyrenoidosa solution;

slow growth period of fruits: watering, fertilizing, preventing plant diseases and insect pests and applying a foliar nutrient solution, wherein the application amount of the foliar nutrient solution is 200-800mL per mu in terms of the protein chlorella solution.

Furthermore, in the fruit tree planting process, the production management of the fruit trees in each growth cycle comprises the following modes:

and (4) nutritional reserve stage: intertilling, weeding, removing root tillers, loosening soil, preserving soil moisture and applying fertilizer;

a dormant period: trimming and pruning, applying root mixed nutrient solution to the roots of the fruit trees along with water during watering, wherein the application amount is 200-400mL per mu in terms of protein chlorella solution;

and (3) during a recovery period: coating the tree body with water;

and (3) in the sprouting stage: clearing the garden;

the growth period of the new branch is as follows: disease prevention, fertilization, and application of a root mixed nutrient solution and a leaf surface nutrient solution, wherein the application amount of the root mixed nutrient solution and the leaf surface nutrient solution is 200-400mL per mu in terms of chlorella vulgaris solution;

and (3) flowering period: bee releasing to promote pollination;

growth period of young fruits: pruning to reduce nutrient loss;

slow growth period of fruits: watering, fertilizing, weeding, preventing plant diseases and insect pests and applying a foliar nutrient solution, wherein the application amount of the foliar nutrient solution is 200-800mL per mu in terms of the protein chlorella solution;

flower bud differentiation and fruit expansion period: watering, pruning and fertilizing.

In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:

1. compared with the conventional cultivation technology of fruit trees, the method can obviously improve the yield of the fruit trees, thereby greatly improving the yield of the orchard under the same condition and increasing the income of fruit growers;

2. by adopting the cultivation technology to manage and plant the fruit trees, the sugar degree of the fruits can be effectively improved, and the taste of the fruits is changed;

3. the invention can obviously reduce the pesticide residue in the fruits, thereby reducing the amount of toxic substances entering human bodies along with the fruits and improving the edible safety of the fruits;

4. the polyunsaturated fatty acid content in the fruit can be greatly increased, and the polyunsaturated fatty acid has the functions of keeping the relative fluidity of cell membranes, ensuring the normal physiological function of cells, esterifying cholesterol, reducing the content of cholesterol and triglyceride in blood, improving blood microcirculation, improving the activity of brain cells and enhancing memory and thinking ability, so that the fruit is delicious in taste when being eaten and also has great benefits for human bodies after being eaten;

5. the fruit trees are managed by adopting the cultivation technology, so that the yield of the fruit trees can be greatly increased, the basic benefit of fruit growers is guaranteed, the sugar content and the content of polyunsaturated fatty acid of the fruits can be greatly improved, the pesticide residue is reduced, the fruit trees can be completely distinguished from common fruits, and the cultivation technology has remarkable significance for creating self brands and enhancing the competitiveness of the fruit growers or planting areas.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a microscope image of the hawthorn produced in example 4.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.

It is to be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

The invention provides a fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae, which comprises the following specific steps:

the root mixed nutrient solution and the foliar nutrient solution are prepared and applied in the planting process of fruit trees, wherein the root mixed nutrient solution and the foliar nutrient solution of the invention can be used for substantially all fruit trees, such as hawthorn, cherry, grape, apple, mango, orange and the like, of course, these fruit trees are only examples, and other fruit trees can also be used. In addition, the root mixed nutrient solution comprises nitrogen-fixing blue algae and photosynthetic nutrient green algae, and the leaf surface nutrient solution comprises photosynthetic nutrient green algae.

Wherein the nitrogen-fixing blue algae can be common microalgae such as anabaena flos-aquae and anabaena azotoformis, and the photosynthetic nutrient green algae can be microalgae such as chlorella pyrenoidosa. Only, experiments show that the best effect of selecting nitrogen-fixing anabaena as the nitrogen-fixing blue algae and the best effect of selecting chlorella pyrenoidosa as the photosynthetic nutrient green algae.

The anabaena azotica fixes nitrogen in the air and converts the nitrogen in the air into nitrogen nutrition which can be absorbed by fruit trees. The chlorella vulgaris has strong light and effect, can improve the sugar degree of fruits, contains C.G.F factor, is also called cell repair factor, can repair damaged cells of crops, and has good barrier effect on heavy metal salt. In addition, the anabaena azotoformans entering the soil can be wound around the roots of crops like winding filaments, the roots are deep, the anabaena azotoformans can be wound deep, and meanwhile, asexual propagation fission is carried out to activate nutrient substances in the soil; the chlorella pyrenoidosa can enter roots, stems, leaves and fruits of crops to play a role, and particularly can enter the epidermis of the fruits through pores on the fruits after being sprayed on the surfaces of fruit trees, so that the fruits contain the chlorella pyrenoidosa, and the effects of improving the immunity of human bodies, reducing cholesterol, improving blood microcirculation, improving brain cell activity and the like can be achieved after the fruits are eaten.

The root mixed nutrient solution comprises a nitrogen fixing blue algae solution and a photosynthetic nutrient green algae solution, the volume ratio of the nitrogen fixing blue algae solution to the photosynthetic nutrient green algae solution is 1-5:1, purified water and the like can be added for dilution, the use is convenient, the using amount of the purified water can be adjusted according to actual requirements, the leaf surface nutrient solution comprises the photosynthetic nutrient green algae solution, purified water and the like can also be added for dilution, the use is convenient, the number of the nitrogen fixing blue algae in the nitrogen fixing blue algae solution is more than or equal to 500 ten thousand/mL, and the number of the photosynthetic nutrient green algae in the photosynthetic nutrient green algae solution is more than or equal to 500 ten thousand/mL. The root mixed nutrient solution is applied in the dormant period and/or the new branch growing period of the fruit tree, and the leaf surface nutrient solution is applied in the new branch growing period and/or the fruit slow growing period of the fruit tree.

The nitrogen-fixing blue algae liquid and the photosynthetic nutrient green algae liquid are obtained by culturing nitrogen-fixing blue algae and photosynthetic nutrient green algae in a culture medium, wherein the culture medium comprises purified water and components with the following concentrations in the purified water: 11-15mg/L C₁₀H₁₆N₂O₈、11-15mg/L C₆H₈O₇、0.11-0.15mg/L FeSO₄·7H₂O、0.11-0.15g/L NaHCO₃、0.11-0.15g/L CaCl₂、0.11-0.15g/L MgSO₄·7H₂O、0.55-0.75g/LK₂HPO₄、0.11-0.15g/L KH₂PO₄、11-15mg/L MnCl₂·4H₂O、0.11-0.15mg/L ZnSO₄·7H₂O、0.11-0.15mg/L CuSO₄·5H₂O、0.11-0.15mg/L Na₂MoO₄·2H₂O、11-15mg/L H₃BO₃11-15mg/L pinellia agglutinin protein.

Wherein the pH value of the water used in the culture medium is 7.5-8.5, the TDS value is 19-21, and when the nitrogen-fixing blue algae/photosynthetic nutrition green algae are put into the culture medium for culture, the illumination is continued and purified air is introduced, the pressure of the purified air is 0.5-0.7MPa, the illumination intensity is 2500-.

In addition, during the planting period of the fruit trees, the production management of the fruit trees in each growth cycle (taking hawthorn as an example, the nutrient reserve period after every year of hawthorn picking to the mature picking period of the second year is a growth cycle) comprises the following modes:

and (4) nutritional reserve stage: cultivating and fertilizing;

a dormant period: applying 400mL of root mixed nutrient solution to the roots of the fruit trees, wherein the application amount is 200-;

the growth period of the new branch is as follows: disease prevention, fertilization, and application of a root mixed nutrient solution and a leaf surface nutrient solution, wherein the application amount of the root mixed nutrient solution and the leaf surface nutrient solution is 400mL per mu in terms of chlorella pyrenoidosa solution;

slow growth period of fruits: watering, fertilizing, preventing plant diseases and insect pests and applying a foliar nutrient solution, wherein the application amount of the foliar nutrient solution is 800mL (which can be applied in 2 times, and 400mL each time) per mu in terms of the protein chlorella solution.

More specifically, the management mode in each growth cycle of the fruit tree is as follows:

and (4) nutritional reserve stage: cultivating, weeding, removing root tillers, loosening soil, preserving soil moisture, applying fertilizer mainly in the nutrient reserve stage, and applying in annular furrow, strip furrow and radial furrow;

a dormant period: trimming and pruning, applying root mixed nutrient solution to the roots of the fruit trees along with water during watering, wherein the application amount is 200-400mL per mu in terms of protein chlorella solution;

and (3) during a recovery period: coating the tree body with water;

and (3) in the sprouting stage: clearing the garden;

the growth period of the new branch is as follows: disease prevention, fertilization, and application of a root mixed nutrient solution and a leaf surface nutrient solution, wherein the application amount of the root mixed nutrient solution and the leaf surface nutrient solution is 200-400mL per mu in terms of chlorella vulgaris solution;

and (3) flowering period: bee releasing to promote pollination;

growth period of young fruits: pruning to reduce nutrient loss;

slow growth period of fruits: watering, fertilizing, weeding, preventing plant diseases and insect pests and applying a foliar nutrient solution, wherein the application amount of the foliar nutrient solution is 200-800mL per mu in terms of the protein chlorella solution;

flower bud differentiation and fruit expansion period: watering, pruning and fertilizing.

The features and properties of the present invention will be further described in detail with reference to the following examples, which are for comparison and are illustrated by using hawthorn trees as representative fruit trees, but do not show that the products of the present invention are only used for hawthorn trees.

Example 1

Preparing a culture medium, wherein the prepared culture medium comprises the following components in concentration except purified water: 11mg/L disodium ethylene diamine tetraacetate, 11/L citric acid and 0.11mg/L FeSO₄·7H₂O、0.11g/LNaHCO₃、0.11g/L CaCl₂、0.11g/L MgSO₄·7H₂O、0.55g/L K₂HPO₄、0.11g/L KH₂PO₄、11mg/L MnCl₂·4H₂O、0.11mg/L ZnSO₄·7H₂O、0.11mg/L CuSO₄·5H₂O、0.11mg/LNa₂MoO₄·2H₂O、11mg/L H₃BO₃11mg/L of pinellia agglutinin protein.

Placing Anabaena azotoformans and Chlorella pyrenoidosa into a bucket containing culture medium, culturing for 80-120 days, continuously irradiating, filtering water and air used in the culture process, purifying to reach standard, wherein the pH value of the water is 7.5, the TDS value is 20, and the illumination intensity is 2500 lux; the culture temperature is 27 ℃; purified air is continuously introduced in the culture process, the pressure of the purified air is 0.5Mpa, and the content of each milliliter of cells after culture is more than or equal to 500 million.

Preparing a root mixed nutrient solution and a leaf surface nutrient solution by using a nitrogen fixing cyanobacteria solution and a photosynthetic nutrient cyanobacteria solution which are obtained after culture by using a culture medium, wherein the ratio of anabaena azotica to chlorella pyrenoidosa in the root mixed nutrient solution is 1: 1, the foliar nutrient solution is only prepared from chlorella pyrenoidosa.

After field planting, taking a nutritional reserve period (from 10 late ten to 11 months) to a mature picking period (from 10 early ten) of the second year after hawthorn picking every year as a growth period, wherein a specific management mode in one growth period is as follows:

the main operation contents of the nutrition reserve period (generally 10 to 11 months in the last ten days of each year) of the hawthorn tree are intertillage and fertilization. Namely, the cultivation and weeding are carried out in time in the nutrition reserve period, the root tillers are removed, and the soil is loosened and the soil moisture is preserved. During cultivation, the root is not injured, and on the basis, deep hoeing is better year by year, and the depth is about 15cm generally. When in fertilization, 3000kg of farmyard manure or 200kg of organic bacterial manure is applied to each mu, and 50kg of compound fertilizer with 45 percent of nitrogen, phosphorus and potassium is applied simultaneously to ensure that sufficient nutrition is provided.

In the dormancy stage (crataegus trees are generally 12 months to 1 month in the second year), the main job contents are overwintering water and pruning. And (3) watering overwintering water, applying 200 ml of root mixed nutrient solution to roots along with the water (or diluting the mixed nutrient solution by 100 times with the water and uniformly spraying the mixed nutrient solution to the periphery of the roots), and preventing freezing and moisturizing. Modifying tree form, applying wound healing agent to the overlarge wound, cutting off insect-infected branches and redundant branches, culturing fruiting branch groups, adjusting tree body mechanism, and improving internal illumination condition.

In the recovery period (the hawthorn trees are generally 2 months per year), the main operation content is tree trunk smearing. The tree trunk is coated with antifreezing nutrient solution to promote absorption and antifreezing of tree body, and small amount of pesticide can promote the functions of killing pests and eggs.

In the germination period (the hawthorn tree is generally in the middle 3 months of each year), the main operation content is garden cleaning. 5 percent of lime sulphur mixture is sprayed in the whole garden to kill the worm eggs on the tree trunk and the ground.

During the growth period of new branches (generally, the last 3 months and the middle 4 months of the hawthorn tree in each year), the main operation contents are disease prevention and fertilization. Adopting 2.5 percent of high-efficiency chlorofluorohydrochrysanthemate 1500-fold liquid, 10 percent of imidacloprid 1500-fold liquid, 30 percent of chlorothalonil 3000-fold liquid and 10 percent of pyridaben 3000-fold liquid to prevent plant diseases and insect pests, such as powdery mildew, budworm and aphid before flowering, spraying 200 ml of leaf surface nutrient solution and 200 ml of selenium-rich nutrient solution, and simultaneously applying 200 ml of root mixed nutrient solution at the root along with water.

In the flowering period (generally, the hawthorn trees are in late 4 months of each year), the main operation content is to release peaks in the garden to promote pollination.

In the growth period of young fruits (generally, 5 months per year of hawthorn trees), pruning is mainly carried out in summer, back branches and branch growing branches are removed, nutrition loss is reduced, and trees are ventilated and transparent.

In the slow growth period (generally, the hawthorn trees are from 6 to 8 months of each year), the main operation contents are watering, weeding, pest and disease prevention and fertilization. Watering properly, and applying organic calcium fertilizer when watering; mowing to enable the grass to be not more than 30 cm, covering the ground by weeds, reducing water evaporation and improving organic matters; in the middle ten days of 6 months, using 1500 times of matrine solution, 20% of acetamiprid 1500 times of solution, 3000 times of pyridaben solution or 43% of tebuconazole 3000 times of solution to prevent and control plant diseases and insect pests, and spraying selenium-enriched nutrient solution; applying 3000 times solution of 5% emamectin benzoate, 3000 times solution of 10% benoxaconazole and 20% in late 6-month ten days to 7 monthsPyridaben 3000 times liquid, selenium-rich nutrient solution, and spray

Acetic acid prevents fruit drop; applying the foliar nutrient solution for 2 times to 200 ml of foliar nutrient solution in the late 7 th to the early 8 th months, promoting growth, enhancing photosynthesis and improving quality.

In the flower bud differentiation and fruit expansion period (generally in the middle and last ten days of 8 months of each year), the main operation contents in the period are proper watering, mowing and covering the land, cutting off redundant branches and solving the problems of ventilation and light transmission; spraying 5% of emamectin benzoate 3000 liquid, 10% of benconazole 3000-fold liquid, 20% of pyridaben 3000-fold liquid and selenium-enriched nutrient solution, and adding

Acetic acid prevents fruit drop. The fruit expanding period is 9 months per year, the main operation content in the period is weeding, watering is properly carried out, and high-potassium water-soluble fertilizer is applied, wherein the dosage per mu is 10 kg.

Example 2

Preparing a culture medium, wherein the prepared culture medium comprises the following components in concentration except purified water: 11mg/L disodium ethylene diamine tetraacetate, 11/L citric acid and 0.11mg/L FeSO₄·7H₂O、0.11g/LNaHCO₃、0.11g/L CaCl₂、0.11g/L MgSO₄·7H₂O、0.55g/L K₂HPO₄、0.11g/L KH₂PO₄、11mg/L MnCl₂·4H₂O、0.11mg/L ZnSO₄·7H₂O、0.11mg/L CuSO₄·5H₂O、0.11mg/LNa₂MoO₄·2H₂O、11mg/L H₃BO₃11mg/L of pinellia agglutinin protein.

Placing Anabaena azotoformans and Chlorella pyrenoidosa into a bucket containing culture medium, culturing for 80-120 days, continuously irradiating, filtering water and air used in the culture process, purifying to reach standard, wherein the pH value of the water is 7.5, the TDS value is 20, and the illumination intensity is 2500 lux; the culture temperature is 27 ℃; purified air is continuously introduced in the culture process, the pressure of the purified air is 0.5Mpa, and the content of each milliliter of cells after culture is more than or equal to 500 million.

Preparing a root mixed nutrient solution and a leaf surface nutrient solution by using a nitrogen fixing cyanobacteria solution and a photosynthetic nutrient cyanobacteria solution which are obtained after culture by using a culture medium, wherein the ratio of anabaena azotica to chlorella pyrenoidosa in the root mixed nutrient solution is 1: 1, the foliar nutrient solution is only prepared from chlorella pyrenoidosa.

After field planting, taking a nutritional reserve period (from 10 late ten to 11 months) to a mature picking period (from 10 early ten) of the second year after hawthorn picking every year as a growth period, wherein a specific management mode in one growth period is as follows:

the main operation contents of the nutrition reserve period (generally 10 to 11 months in the last ten days of each year) of the hawthorn tree are intertillage and fertilization. Namely, the cultivation and weeding are carried out in time in the nutrition reserve period, the root tillers are removed, and the soil is loosened and the soil moisture is preserved. During cultivation, the root is not injured, and on the basis, deep hoeing is better year by year, and the depth is about 15cm generally. When in fertilization, 3000kg of farmyard manure or 200kg of organic bacterial manure is applied to each mu, and 50kg of compound fertilizer with 45 percent of nitrogen, phosphorus and potassium is applied simultaneously to ensure that sufficient nutrition is provided.

In the dormancy stage (crataegus trees are generally 12 months to 1 month in the second year), the main job contents are overwintering water and pruning. And (3) watering overwintering water, applying 400ml of root mixed nutrient solution to roots along with the water (or diluting the mixed nutrient solution by 100 times with the water and uniformly spraying the mixed nutrient solution to the periphery of the roots), and preventing freezing and moisturizing. Modifying tree form, applying wound healing agent to the overlarge wound, cutting off insect-infected branches and redundant branches, culturing fruiting branch groups, adjusting tree body mechanism, and improving internal illumination condition.

In the recovery period (the hawthorn trees are generally 2 months per year), the main operation content is tree trunk smearing. The tree trunk is coated with antifreezing nutrient solution to promote absorption and antifreezing of tree body, and small amount of pesticide can promote the functions of killing pests and eggs.

In the germination period (the hawthorn tree is generally in the middle 3 months of each year), the main operation content is garden cleaning. 5 percent of lime sulphur mixture is sprayed in the whole garden to kill the worm eggs on the tree trunk and the ground.

During the growth period of new branches (generally, the last 3 months and the middle 4 months of the hawthorn tree in each year), the main operation contents are disease prevention and fertilization. Adopting 2.5 percent of high-efficiency chlorofluorohydrochrysanthemate 1500-fold liquid, 10 percent of imidacloprid 1500-fold liquid, 30 percent of chlorothalonil 3000-fold liquid and 10 percent of pyridaben 3000-fold liquid to prevent plant diseases and insect pests, such as powdery mildew, budworm and aphid before flowering, spraying 400ml of leaf surface nutrient solution and 400ml of selenium-rich nutrient solution, and simultaneously applying 400ml of root mixed nutrient solution at the root along with water.

In the flowering period (generally, the hawthorn trees are in late 4 months of each year), the main operation content is to release peaks in the garden to promote pollination.

In the growth period of young fruits (generally, 5 months per year of hawthorn trees), pruning is mainly carried out in summer, back branches and branch growing branches are removed, nutrition loss is reduced, and trees are ventilated and transparent.

In the slow growth period (generally, the hawthorn trees are from 6 to 8 months of each year), the main operation contents are watering, weeding, pest and disease prevention and fertilization. Watering properly, and applying organic calcium fertilizer when watering; mowing to enable the grass to be not more than 30 cm, covering the ground by weeds, reducing water evaporation and improving organic matters; in the middle ten days of 6 months, using 1500 times of matrine solution, 20% of acetamiprid 1500 times of solution, 3000 times of pyridaben solution or 43% of tebuconazole 3000 times of solution to prevent and control plant diseases and insect pests, and spraying selenium-enriched nutrient solution; applying 3000 times of 5% emamectin benzoate, 3000 times of 10% benacazole, 3000 times of 20% pyridaben and selenium-rich nutrient solution in late 6-month ten to 7-month day, and spraying

Acetic acid prevents fruit drop; applying the foliar nutrient solution for 2 times, 400ml each time, on the foliar surfaces from late 7 th to early 8 th, promoting growth, enhancing photosynthesis and improving quality.

In the flower bud differentiation and fruit expansion period (generally in the middle and last ten days of 8 months of each year), the main operation contents in the period are proper watering, mowing and covering the land, cutting off redundant branches and solving the problems of ventilation and light transmission; spraying 5% of emamectin benzoate 3000 liquid, 10% of benconazole 3000-fold liquid, 20% of pyridaben 3000-fold liquid and selenium-enriched nutrient solution, and adding

Acetic acid prevents fruit drop. The fruit expanding period is 9 months per year, and the main operation content in the period is weedingProperly watering and applying high-potassium water-soluble fertilizer, wherein the dosage per mu is 10 kg.

Example 3

Preparing a culture medium, wherein the prepared culture medium comprises the following components in concentration except purified water: 11mg/L disodium ethylene diamine tetraacetate, 11/L citric acid and 0.11mg/L FeSO₄·7H₂O、0.11g/LNaHCO₃、0.11g/L CaCl₂、0.11g/L MgSO₄·7H₂O、0.55g/L K₂HPO₄、0.11g/L KH₂PO₄、11mg/L MnCl₂·4H₂O、0.11mg/L ZnSO₄·7H₂O、0.11mg/L CuSO₄·5H₂O、0.11mg/LNa₂MoO₄·2H₂O、11mg/L H₃BO₃11mg/L of pinellia agglutinin protein.

Placing Anabaena azotoformans and Chlorella pyrenoidosa into a bucket containing culture medium, culturing for 80-120 days, continuously irradiating, filtering water and air used in the culture process, purifying to reach standard, wherein the pH value of the water is 7.5, the TDS value is 20, and the illumination intensity is 2500 lux; the culture temperature is 27 ℃; purified air is continuously introduced in the culture process, the pressure of the purified air is 0.5Mpa, and the content of each milliliter of cells after culture is more than or equal to 500 million.

Preparing a root mixed nutrient solution and a leaf surface nutrient solution by using a nitrogen fixing cyanobacteria solution and a photosynthetic nutrient cyanobacteria solution which are obtained after culture by using a culture medium, wherein the ratio of anabaena azotica to chlorella pyrenoidosa in the root mixed nutrient solution is 2: 1, the foliar nutrient solution is only prepared from chlorella pyrenoidosa.

After field planting, taking a nutritional reserve period (from 10 late ten to 11 months) to a mature picking period (from 10 early ten) of the second year after hawthorn picking every year as a growth period, wherein a specific management mode in one growth period is as follows:

the main operation contents of the nutrition reserve period (generally 10 to 11 months in the last ten days of each year) of the hawthorn tree are intertillage and fertilization. Namely, the cultivation and weeding are carried out in time in the nutrition reserve period, the root tillers are removed, and the soil is loosened and the soil moisture is preserved. During cultivation, the root is not injured, and on the basis, deep hoeing is better year by year, and the depth is about 15cm generally. When in fertilization, 3000kg of farmyard manure or 200kg of organic bacterial manure is applied to each mu, and 50kg of compound fertilizer with 45 percent of nitrogen, phosphorus and potassium is applied simultaneously to ensure that sufficient nutrition is provided.

In the dormancy stage (crataegus trees are generally 12 months to 1 month in the second year), the main job contents are overwintering water and pruning. And (3) watering overwintering water, applying 400ml of root mixed nutrient solution to roots along with the water (or diluting the mixed nutrient solution by 100 times with the water and uniformly spraying the mixed nutrient solution to the periphery of the roots), and preventing freezing and moisturizing. Modifying tree form, applying wound healing agent to the overlarge wound, cutting off insect-infected branches and redundant branches, culturing fruiting branch groups, adjusting tree body mechanism, and improving internal illumination condition.

In the recovery period (the hawthorn trees are generally 2 months per year), the main operation content is tree trunk smearing. The tree trunk is coated with antifreezing nutrient solution to promote absorption and antifreezing of tree body, and small amount of pesticide can promote the functions of killing pests and eggs.

In the germination period (the hawthorn tree is generally in the middle 3 months of each year), the main operation content is garden cleaning. 5 percent of lime sulphur mixture is sprayed in the whole garden to kill the worm eggs on the tree trunk and the ground.

During the growth period of new branches (generally, the last 3 months and the middle 4 months of the hawthorn tree in each year), the main operation contents are disease prevention and fertilization. Adopting 2.5 percent of high-efficiency chlorofluorohydrochrysanthemate 1500-fold liquid, 10 percent of imidacloprid 1500-fold liquid, 30 percent of chlorothalonil 3000-fold liquid and 10 percent of pyridaben 3000-fold liquid to prevent plant diseases and insect pests, such as powdery mildew, budworm and aphid before flowering, spraying 400ml of leaf surface nutrient solution and 400ml of selenium-rich nutrient solution, and simultaneously applying 400ml of root mixed nutrient solution at the root along with water.

In the flowering period (generally, the hawthorn trees are in late 4 months of each year), the main operation content is to release peaks in the garden to promote pollination.

In the growth period of young fruits (generally, 5 months per year of hawthorn trees), pruning is mainly carried out in summer, back branches and branch growing branches are removed, nutrition loss is reduced, and trees are ventilated and transparent.

In the slow growth period (generally, the hawthorn trees are from 6 to 8 months of each year), the main operation contents are watering, weeding, pest and disease prevention and fertilization. Watering properly, and applying organic calcium fertilizer when watering; mowing to make grass not surpass30 cm, the weeds cover the ground, so that the evaporation of water is reduced, and organic matters are improved; in the middle ten days of 6 months, using 1500 times of matrine solution, 20% of acetamiprid 1500 times of solution, 3000 times of pyridaben solution or 43% of tebuconazole 3000 times of solution to prevent and control plant diseases and insect pests, and spraying selenium-enriched nutrient solution; applying 3000 times of 5% emamectin benzoate, 3000 times of 10% benacazole, 3000 times of 20% pyridaben and selenium-rich nutrient solution in late 6-month ten to 7-month day, and spraying

Acetic acid prevents fruit drop; applying the foliar nutrient solution for 2 times, 400ml each time, on the foliar surfaces from late 7 th to early 8 th, promoting growth, enhancing photosynthesis and improving quality.

In the flower bud differentiation and fruit expansion period (generally in the middle and last ten days of 8 months of each year), the main operation contents in the period are proper watering, mowing and covering the land, cutting off redundant branches and solving the problems of ventilation and light transmission; spraying 5% of emamectin benzoate 3000 liquid, 10% of benconazole 3000-fold liquid, 20% of pyridaben 3000-fold liquid and selenium-enriched nutrient solution, and adding

Acetic acid prevents fruit drop. The fruit expanding period is 9 months per year, the main operation content in the period is weeding, watering is properly carried out, and high-potassium water-soluble fertilizer is applied, wherein the dosage per mu is 10 kg.

Example 4

Preparing a culture medium, wherein the prepared culture medium comprises the following components in concentration except purified water: 11mg/L disodium ethylene diamine tetraacetate, 11/L citric acid and 0.11mg/L FeSO₄·7H₂O、0.11g/LNaHCO₃、0.11g/L CaCl₂、0.11g/L MgSO₄·7H₂O、0.55g/L K₂HPO₄、0.11g/L KH₂PO₄、11mg/L MnCl₂·4H₂O、0.11mg/L ZnSO₄·7H₂O、0.11mg/L CuSO₄·5H₂O、0.11mg/LNa₂MoO₄·2H₂O、11mg/L H₃BO₃11mg/L of pinellia agglutinin protein.

Placing Anabaena azotoformans and Chlorella pyrenoidosa into a bucket containing culture medium, culturing for 80-120 days, continuously irradiating, filtering water and air used in the culture process, purifying to reach standard, wherein the pH value of the water is 7.5, the TDS value is 20, and the illumination intensity is 2500 lux; the culture temperature is 27 ℃; purified air is continuously introduced in the culture process, the pressure of the purified air is 0.5Mpa, and the content of each milliliter of cells after culture is more than or equal to 500 million.

Preparing a root mixed nutrient solution and a leaf surface nutrient solution by using a nitrogen fixing cyanobacteria solution and a photosynthetic nutrient cyanobacteria solution which are obtained after culture by using a culture medium, wherein the ratio of anabaena azotica to chlorella pyrenoidosa in the root mixed nutrient solution is 3: 1, the foliar nutrient solution is only prepared from chlorella pyrenoidosa.

After field planting, taking a nutritional reserve period (from 10 late ten to 11 months) to a mature picking period (from 10 early ten) of the second year after hawthorn picking every year as a growth period, wherein a specific management mode in one growth period is as follows:

the main operation contents of the nutrition reserve period (generally 10 to 11 months in the last ten days of each year) of the hawthorn tree are intertillage and fertilization. Namely, the cultivation and weeding are carried out in time in the nutrition reserve period, the root tillers are removed, and the soil is loosened and the soil moisture is preserved. During cultivation, the root is not injured, and on the basis, deep hoeing is better year by year, and the depth is about 15cm generally. When in fertilization, 3000kg of farmyard manure or 200kg of organic bacterial manure is applied to each mu, and 50kg of compound fertilizer with 45 percent of nitrogen, phosphorus and potassium is applied simultaneously to ensure that sufficient nutrition is provided.

In the dormancy stage (crataegus trees are generally 12 months to 1 month in the second year), the main job contents are overwintering water and pruning. And (3) watering overwintering water, applying 400ml of root mixed nutrient solution to roots along with the water (or diluting the mixed nutrient solution by 100 times with the water and uniformly spraying the mixed nutrient solution to the periphery of the roots), and preventing freezing and moisturizing. Modifying tree form, applying wound healing agent to the overlarge wound, cutting off insect-infected branches and redundant branches, culturing fruiting branch groups, adjusting tree body mechanism, and improving internal illumination condition.

In the recovery period (the hawthorn trees are generally 2 months per year), the main operation content is tree trunk smearing. The tree trunk is coated with antifreezing nutrient solution to promote absorption and antifreezing of tree body, and small amount of pesticide can promote the functions of killing pests and eggs.

In the germination period (the hawthorn tree is generally in the middle 3 months of each year), the main operation content is garden cleaning. 5 percent of lime sulphur mixture is sprayed in the whole garden to kill the worm eggs on the tree trunk and the ground.

During the growth period of new branches (generally, the last 3 months and the middle 4 months of the hawthorn tree in each year), the main operation contents are disease prevention and fertilization. Adopting 2.5 percent of high-efficiency chlorofluorohydrochrysanthemate 1500-fold liquid, 10 percent of imidacloprid 1500-fold liquid, 30 percent of chlorothalonil 3000-fold liquid and 10 percent of pyridaben 3000-fold liquid to prevent plant diseases and insect pests, such as powdery mildew, budworm and aphid before flowering, spraying 400ml of leaf surface nutrient solution and 400ml of selenium-rich nutrient solution, and simultaneously applying 400ml of root mixed nutrient solution at the root along with water.

In the flowering period (generally, the hawthorn trees are in late 4 months of each year), the main operation content is to release peaks in the garden to promote pollination.

In the growth period of young fruits (generally, 5 months per year of hawthorn trees), pruning is mainly carried out in summer, back branches and branch growing branches are removed, nutrition loss is reduced, and trees are ventilated and transparent.

In the slow growth period (generally, the hawthorn trees are from 6 to 8 months of each year), the main operation contents are watering, weeding, pest and disease prevention and fertilization. Watering properly, and applying organic calcium fertilizer when watering; mowing to enable the grass to be not more than 30 cm, covering the ground by weeds, reducing water evaporation and improving organic matters; in the middle ten days of 6 months, using 1500 times of matrine solution, 20% of acetamiprid 1500 times of solution, 3000 times of pyridaben solution or 43% of tebuconazole 3000 times of solution to prevent and control plant diseases and insect pests, and spraying selenium-enriched nutrient solution; applying 3000 times of 5% emamectin benzoate, 3000 times of 10% benacazole, 3000 times of 20% pyridaben and selenium-rich nutrient solution in late 6-month ten to 7-month day, and spraying

Acetic acid prevents fruit drop; applying the foliar nutrient solution for 2 times, 400ml each time, on the foliar surfaces from late 7 th to early 8 th, promoting growth, enhancing photosynthesis and improving quality.

In the flower bud differentiation and fruit expansion period (generally in the middle and last ten days of 8 months of each year), the main operation contents in the period are proper watering, mowing and covering the land, cutting off redundant branches and solving the problems of ventilation and light transmission; spraying 5% Jiawei3000 parts of salt solution, 3000 parts of 10% of difenoconazole solution, 3000 parts of 20% of pyridaben solution and selenium-enriched nutrient solution, and spraying

Acetic acid prevents fruit drop. The fruit expanding period is 9 months per year, the main operation content in the period is weeding, watering is properly carried out, and high-potassium water-soluble fertilizer is applied, wherein the dosage per mu is 10 kg.

Example 5

Preparing a culture medium, wherein the prepared culture medium comprises the following components in concentration except purified water: 11mg/L disodium ethylene diamine tetraacetate, 11/L citric acid and 0.11mg/L FeSO₄·7H₂O、0.11g/LNaHCO₃、0.11g/L CaCl₂、0.11g/L MgSO₄·7H₂O、0.55g/L K₂HPO₄、0.11g/L KH₂PO₄、11mg/L MnCl₂·4H₂O、0.11mg/L ZnSO₄·7H₂O、0.11mg/L CuSO₄·5H₂O、0.11mg/LNa₂MoO₄·2H₂O、11mg/L H₃BO₃11mg/L of pinellia agglutinin protein.

Placing Anabaena azotoformans and Chlorella pyrenoidosa into a bucket containing culture medium, culturing for 80-120 days, continuously irradiating, filtering water and air used in the culture process, purifying to reach standard, wherein the pH value of the water is 7.5, the TDS value is 20, and the illumination intensity is 2500 lux; the culture temperature is 27 ℃; purified air is continuously introduced in the culture process, the pressure of the purified air is 0.5Mpa, and the content of each milliliter of cells after culture is more than or equal to 500 million.

Preparing a root mixed nutrient solution and a leaf surface nutrient solution by using a nitrogen fixing cyanobacteria solution and a photosynthetic nutrient cyanobacteria solution which are obtained after culture by using a culture medium, wherein the ratio of anabaena azotica to chlorella pyrenoidosa in the root mixed nutrient solution is 4: 1, the foliar nutrient solution is only prepared from chlorella pyrenoidosa.

After field planting, taking a nutritional reserve period (from 10 late ten to 11 months) to a mature picking period (from 10 early ten) of the second year after hawthorn picking every year as a growth period, wherein a specific management mode in one growth period is as follows:

the main operation contents of the nutrition reserve period (generally 10 to 11 months in the last ten days of each year) of the hawthorn tree are intertillage and fertilization. Namely, the cultivation and weeding are carried out in time in the nutrition reserve period, the root tillers are removed, and the soil is loosened and the soil moisture is preserved. During cultivation, the root is not injured, and on the basis, deep hoeing is better year by year, and the depth is about 15cm generally. When in fertilization, 3000kg of farmyard manure or 200kg of organic bacterial manure is applied to each mu, and 50kg of compound fertilizer with 45 percent of nitrogen, phosphorus and potassium is applied simultaneously to ensure that sufficient nutrition is provided.

In the dormancy stage (crataegus trees are generally 12 months to 1 month in the second year), the main job contents are overwintering water and pruning. And (3) watering overwintering water, applying 400ml of root mixed nutrient solution to roots along with the water (or diluting the mixed nutrient solution by 100 times with the water and uniformly spraying the mixed nutrient solution to the periphery of the roots), and preventing freezing and moisturizing. Modifying tree form, applying wound healing agent to the overlarge wound, cutting off insect-infected branches and redundant branches, culturing fruiting branch groups, adjusting tree body mechanism, and improving internal illumination condition.

In the recovery period (the hawthorn trees are generally 2 months per year), the main operation content is tree trunk smearing. The tree trunk is coated with antifreezing nutrient solution to promote absorption and antifreezing of tree body, and small amount of pesticide can promote the functions of killing pests and eggs.

In the germination period (the hawthorn tree is generally in the middle 3 months of each year), the main operation content is garden cleaning. 5 percent of lime sulphur mixture is sprayed in the whole garden to kill the worm eggs on the tree trunk and the ground.

During the growth period of new branches (generally, the last 3 months and the middle 4 months of the hawthorn tree in each year), the main operation contents are disease prevention and fertilization. Adopting 2.5 percent of high-efficiency chlorofluorohydrochrysanthemate 1500-fold liquid, 10 percent of imidacloprid 1500-fold liquid, 30 percent of chlorothalonil 3000-fold liquid and 10 percent of pyridaben 3000-fold liquid to prevent plant diseases and insect pests, such as powdery mildew, budworm and aphid before flowering, spraying 400ml of leaf surface nutrient solution and 400ml of selenium-rich nutrient solution, and simultaneously applying 400ml of root mixed nutrient solution at the root along with water.

In the flowering period (generally, the hawthorn trees are in late 4 months of each year), the main operation content is to release peaks in the garden to promote pollination.

In the growth period of young fruits (generally, 5 months per year of hawthorn trees), pruning is mainly carried out in summer, back branches and branch growing branches are removed, nutrition loss is reduced, and trees are ventilated and transparent.

In the slow growth period (generally, the hawthorn trees are from 6 to 8 months of each year), the main operation contents are watering, weeding, pest and disease prevention and fertilization. Watering properly, and applying organic calcium fertilizer when watering; mowing to enable the grass to be not more than 30 cm, covering the ground by weeds, reducing water evaporation and improving organic matters; in the middle ten days of 6 months, using 1500 times of matrine solution, 20% of acetamiprid 1500 times of solution, 3000 times of pyridaben solution or 43% of tebuconazole 3000 times of solution to prevent and control plant diseases and insect pests, and spraying selenium-enriched nutrient solution; applying 3000 times of 5% emamectin benzoate, 3000 times of 10% benacazole, 3000 times of 20% pyridaben and selenium-rich nutrient solution in late 6-month ten to 7-month day, and spraying

Acetic acid prevents fruit drop; applying the foliar nutrient solution for 2 times, 400ml each time, on the foliar surfaces from late 7 th to early 8 th, promoting growth, enhancing photosynthesis and improving quality.

In the flower bud differentiation and fruit expansion period (generally in the middle and last ten days of 8 months of each year), the main operation contents in the period are proper watering, mowing and covering the land, cutting off redundant branches and solving the problems of ventilation and light transmission; spraying 5% of emamectin benzoate 3000 liquid, 10% of benconazole 3000-fold liquid, 20% of pyridaben 3000-fold liquid and selenium-enriched nutrient solution, and adding

Acetic acid prevents fruit drop. The fruit expanding period is 9 months per year, the main operation content in the period is weeding, watering is properly carried out, and high-potassium water-soluble fertilizer is applied, wherein the dosage per mu is 10 kg.

Example 6

Preparing a culture medium, wherein the prepared culture medium comprises the following components in concentration except purified water: 11mg/L disodium ethylene diamine tetraacetate, 11/L citric acid and 0.11mg/L FeSO₄·7H₂O、0.11g/LNaHCO₃、0.11g/L CaCl₂、0.11g/L MgSO₄·7H₂O、0.55g/L K₂HPO₄、0.11g/L KH₂PO₄、11mg/L MnCl₂·4H₂O、0.11mg/L ZnSO₄·7H₂O、0.11mg/L CuSO₄·5H₂O、0.11mg/LNa₂MoO₄·2H₂O、11mg/L H₃BO₃11mg/L of pinellia agglutinin protein.

Placing Anabaena azotoformans and Chlorella pyrenoidosa into a bucket containing culture medium, culturing for 80-120 days, continuously irradiating, filtering water and air used in the culture process, purifying to reach standard, wherein the pH value of the water is 7.5, the TDS value is 20, and the illumination intensity is 2500 lux; the culture temperature is 27 ℃; purified air is continuously introduced in the culture process, the pressure of the purified air is 0.5Mpa, and the content of each milliliter of cells after culture is more than or equal to 500 million.

Preparing a root mixed nutrient solution and a leaf surface nutrient solution by using a nitrogen fixing cyanobacteria solution and a photosynthetic nutrient cyanobacteria solution which are obtained after culture by using a culture medium, wherein the ratio of anabaena azotica to chlorella pyrenoidosa in the root mixed nutrient solution is 5:1, the foliar nutrient solution is only prepared from chlorella pyrenoidosa.

After field planting, taking a nutritional reserve period (from 10 late ten to 11 months) to a mature picking period (from 10 early ten) of the second year after hawthorn picking every year as a growth period, wherein a specific management mode in one growth period is as follows:

the main operation contents of the nutrition reserve period (generally 10 to 11 months in the last ten days of each year) of the hawthorn tree are intertillage and fertilization. Namely, the cultivation and weeding are carried out in time in the nutrition reserve period, the root tillers are removed, and the soil is loosened and the soil moisture is preserved. During cultivation, the root is not injured, and on the basis, deep hoeing is better year by year, and the depth is about 15cm generally. When in fertilization, 3000kg of farmyard manure or 200kg of organic bacterial manure is applied to each mu, and 50kg of compound fertilizer with 45 percent of nitrogen, phosphorus and potassium is applied simultaneously to ensure that sufficient nutrition is provided.

In the dormancy stage (crataegus trees are generally 12 months to 1 month in the second year), the main job contents are overwintering water and pruning. And (3) watering overwintering water, applying 400ml of root mixed nutrient solution to roots along with the water (or diluting the mixed nutrient solution by 100 times with the water and uniformly spraying the mixed nutrient solution to the periphery of the roots), and preventing freezing and moisturizing. Modifying tree form, applying wound healing agent to the overlarge wound, cutting off insect-infected branches and redundant branches, culturing fruiting branch groups, adjusting tree body mechanism, and improving internal illumination condition.

In the recovery period (the hawthorn trees are generally 2 months per year), the main operation content is tree trunk smearing. The tree trunk is coated with antifreezing nutrient solution to promote absorption and antifreezing of tree body, and small amount of pesticide can promote the functions of killing pests and eggs.

In the germination period (the hawthorn tree is generally in the middle 3 months of each year), the main operation content is garden cleaning. 5 percent of lime sulphur mixture is sprayed in the whole garden to kill the worm eggs on the tree trunk and the ground.

During the growth period of new branches (generally, the last 3 months and the middle 4 months of the hawthorn tree in each year), the main operation contents are disease prevention and fertilization. Adopting 2.5 percent of high-efficiency chlorofluorohydrochrysanthemate 1500-fold liquid, 10 percent of imidacloprid 1500-fold liquid, 30 percent of chlorothalonil 3000-fold liquid and 10 percent of pyridaben 3000-fold liquid to prevent plant diseases and insect pests, such as powdery mildew, budworm and aphid before flowering, spraying 400ml of leaf surface nutrient solution and 400ml of selenium-rich nutrient solution, and simultaneously applying 400ml of root mixed nutrient solution at the root along with water.

In the flowering period (generally, the hawthorn trees are in late 4 months of each year), the main operation content is to release peaks in the garden to promote pollination.

In the growth period of young fruits (generally, 5 months per year of hawthorn trees), pruning is mainly carried out in summer, back branches and branch growing branches are removed, nutrition loss is reduced, and trees are ventilated and transparent.

In the slow growth period (generally, the hawthorn trees are from 6 to 8 months of each year), the main operation contents are watering, weeding, pest and disease prevention and fertilization. Watering properly, and applying organic calcium fertilizer when watering; mowing to enable the grass to be not more than 30 cm, covering the ground by weeds, reducing water evaporation and improving organic matters; in the middle ten days of 6 months, using 1500 times of matrine solution, 20% of acetamiprid 1500 times of solution, 3000 times of pyridaben solution or 43% of tebuconazole 3000 times of solution to prevent and control plant diseases and insect pests, and spraying selenium-enriched nutrient solution; applying 3000 times of 5% emamectin benzoate, 3000 times of 10% benacazole, 3000 times of 20% pyridaben and selenium-rich nutrient solution in late 6-month ten to 7-month day, and spraying

Acetic acid prevents fruit drop; leaf surface is applied from late 7 to early 8The leaf surface nutrient solution is 400 milliliters every time after 2 times, so that the growth is promoted, the photosynthesis is enhanced, and the quality is improved.

In the flower bud differentiation and fruit expansion period (generally in the middle and last ten days of 8 months of each year), the main operation contents in the period are proper watering, mowing and covering the land, cutting off redundant branches and solving the problems of ventilation and light transmission; spraying 5% of emamectin benzoate 3000 liquid, 10% of benconazole 3000-fold liquid, 20% of pyridaben 3000-fold liquid and selenium-enriched nutrient solution, and adding

Acetic acid prevents fruit drop. The fruit expanding period is 9 months per year, the main operation content in the period is weeding, watering is properly carried out, and high-potassium water-soluble fertilizer is applied, wherein the dosage per mu is 10 kg.

Test examples

Selecting 7 same areas under the same management condition, wherein each area is one mu, 6 mu manages the hawthorn trees according to the mode of the embodiment 1-6, and the rest one mu manages according to the following mode (for short, comparison example):

and (4) nutritional reserve stage: the main operation contents are intertillage and fertilization. And (3) intertilling and weeding in time in a nutrition reserve period, removing root tillers, loosening the soil and preserving the soil moisture. During cultivation, the root is not injured, and on the basis, deep hoeing is better year by year, and the depth is about 15cm generally. The hillside orchard should also be careful to keep water and soil. And fertilizing in time in the nutrition storage period to supplement nutrition. The fertilizer application is mainly carried out on organic fertilizers, 3000kg of farmyard manure or 200kg of organic bacterial manure is applied per mu, and 50kg of compound fertilizers with 45% of nitrogen, phosphorus and potassium contents are applied simultaneously to ensure that sufficient nutrition is provided.

A dormant period: the main operation contents are overwintering water and pruning. Watering overwintering water, modifying tree shape, coating wound healing agent on overlarge wound, cutting off insect-infected branches and redundant branches, culturing fruiting branch groups, adjusting tree body mechanism, and improving internal illumination condition.

And (3) during a recovery period: the main operation content is tree trunk coating. The tree trunk is coated with antifreezing nutrient solution to promote absorption and antifreezing of tree body, and small amount of pesticide can promote the functions of killing pests and eggs.

And (3) in the sprouting stage: the main operation content is clearing the garden. 5% lime sulphur mixture is sprayed in the whole garden to kill the worm eggs on the tree trunk and the ground.

The growth period of the new branch is as follows: the main operation contents are disease prevention and fertilization. Spraying pesticide before flowers to prevent powdery mildew, budworm and aphid, and mainly applying 2.5 percent of high-efficiency chlorofluorohydrochrysanthester 1500-fold liquid, 10 percent of imidacloprid 1500-fold liquid, 30 percent of chlorothalonil 3000-fold liquid, 0 percent of Daxianling 3000-fold liquid and 200 milliliters of selenium-rich nutrient solution.

And (3) flowering period: the main operation content is releasing peaks in the garden to promote pollination.

Growth period of young fruits: the pruning is mainly carried out in summer, the back branches and the spindly branches are removed, the nutrition loss is reduced, and the tree body is ventilated and transparent.

And (3) slow growth period: the main operation contents are watering, weeding, pest and disease prevention and fertilization. Watering properly, and applying organic calcium fertilizer when watering; mowing till the grass is not more than 30 cm, and covering the ground with weeds; 1500 times of matrine solution, 20% of acetamiprid 1500 times of solution, 3000 times of pyridaben or 43% of tebuconazole 3000 times of solution and selenium-enriched nutrient solution are used for preventing and treating diseases and insect pests in the middle ten days of 6 months; applying 3000 times solution of 5% emamectin benzoate, 3000 times solution of 10% benacazole, 3000 times solution of 20% pyridaben and selenium-rich nutrient solution in late 6-month ten to 7-month

Acetic acid prevents fruit drop.

Flower bud differentiation and fruit expansion period: the main operation contents in the period are proper watering, mowing and covering the ground, and cutting off redundant branches to solve the problems of ventilation and light transmission; spraying 5% of emamectin benzoate 3000 liquid, 10% of benconazole 3000-fold liquid, 20% of pyridaben 3000-fold liquid and selenium-enriched nutrient solution

Acetic acid prevents fruit drop. The main operation content in the fruit expanding period is weeding, proper watering is carried out, and 10kg of high-potassium water-soluble fertilizer is applied per mu.

After the hawthorn is ripe, picking the hawthorn fruit to carry out the following detection:

the yield ratios of examples 1 to 6 were calculated relative to the comparative example, and the calculation results are shown in table 1 below:

TABLE 1 yield increase experiment

Item	Haw
		Comparative example	0
Example 1	8.7％
		Example 2	9.1％
Example 3	10.8％
		Example 4	12.5％
Example 5	11.7％
		Example 6	11.3％

As can be seen from Table 1, the yield of hawthorn fruits obtained in examples 1-6 is obviously increased compared with the comparative example, and the effect of increasing the yield of the fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae can be obviously reflected. Meanwhile, the yield increase effect of the embodiment 4 is obvious compared with the embodiments 1, 2, 3, 5 and 6, which shows that 400ml of root mixed nutrient solution is applied to roots and 400ml of leaf nutrient solution is applied to leaves per mu of roots, and the ratio of the nitrogen-fixing anabaena protein chlorella is 3: 1, the yield increasing effect is more obvious.

Randomly selecting 30 mature hawthorns per mu of land, and measuring the average sugar degree of the hawthorns, wherein the measurement results are shown in the following table 2:

TABLE 2 sugar increase experiment (degree)

As can be seen from Table 2, the sugar increasing effect of examples 1-6 is obvious compared with the comparative examples, which reflects that the sugar increasing effect of the fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrition green algae is obvious. Meanwhile, the sugar increasing effect of the embodiment 4 is more obvious than that of the embodiments 1, 2, 3, 5 and 6, which shows that 400ml of root mixed nutrient solution is applied to roots and 400ml of leaf nutrient solution is applied to leaves per mu of roots, and the ratio of the nitrogen-fixing anabaena protein chlorella is 3: 1, the sugar increasing effect is more obvious.

Randomly selecting 30 mature hawthorns per mu of land, measuring the average content of chlorbenzuron and chlordimeform in the hawthorns, and calculating the result as shown in table 3:

TABLE 3 pesticide residue reduction experiment

Item	Chlorbenzuron (g/100g)	Insecticidal amidine (mg/kg)
			Comparative example	0.0235	0.00981
Example 1	Not detected out	Not detected out
			Example 2	Not detected out	Not detected out
Example 3	Not detected out	Not detected out
			Example 4	Not detected out	Not detected out
Example 5	Not detected out	Not detected out
			Example 6	Not detected out	Not detected out

As can be seen from Table 3, the pesticide residue reducing effect of examples 1-6 is very obvious compared with the comparative example, which reflects that the pesticide residue reducing effect of the fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae is obvious and is more beneficial to human health.

Randomly selecting 30 mature hawthorns per mu of land, measuring the average increase of polyunsaturated fatty acids of the hawthorns planted in examples 1-6 relative to the hawthorns planted in comparative examples, and measuring results are shown in table 4:

TABLE 4 increase of unsaturated fatty acid content experiment (g/1kg)

As can be seen from Table 4, the content of polyunsaturated fatty acids in the hawthorns planted in examples 1-6 is obviously increased compared with the hawthorns planted in comparative examples, which can reflect that the increasing effect of the fruit cultivation technology based on nitrogen-fixing fish blue algae and photosynthetic nutrient green algae on the content of polyunsaturated fatty acids is obvious. Meanwhile, the polyunsaturated fatty acid increase effect of example 4 is more obvious than that of examples 1, 2, 3, 5 and 6, which shows that 400ml of root mixed nutrient solution and 400ml of leaf nutrient solution are applied to the leaf surface of each mu of roots, and the ratio of the anabaena azotoformans protein chlorella is 3: 1, the increase effect on the polyunsaturated fatty acid is more obvious.

In addition, the skin structure of the mature hawthorn in the embodiment 4 is observed by a microscope, the specific structure diagram is shown in fig. 1, and it can be seen from the diagram that the hawthorn skin contains a large amount of chlorella pyrenoidosa, so that the hawthorn not only has a more excellent health-care effect, but also can be further distinguished from the common hawthorn, and is convenient for fruit growers to form unique characteristic agricultural products.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims (9)

1. A fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae is characterized in that during the planting process of fruit trees, a root mixed nutrient solution is applied to the roots of the fruit trees, and a leaf surface nutrient solution is applied to the leaf surfaces of the fruit trees, wherein the root mixed nutrient solution comprises the nitrogen-fixing blue algae and the photosynthetic nutrient green algae, and the leaf surface nutrient solution comprises the photosynthetic nutrient green algae.

2. The technology for cultivating fruit trees based on nitrogen-fixing cyanobacteria and photosynthetic nutritious green algae according to claim 1, wherein the root mixed nutrient solution is applied in the dormancy stage and/or the growth stage of new branches of the fruit trees, and the foliar nutrient solution is applied in the growth stage of new branches of the fruit trees and/or the slow growth stage of fruits.

3. The fruit tree cultivation technology based on nitrogen-fixing blue-green algae and photosynthetic nutrient green algae as claimed in claim 1, wherein the root mixed nutrient solution comprises nitrogen-fixing blue-green algae solution and photosynthetic nutrient green algae solution, and the volume ratio of the nitrogen-fixing blue-green algae solution to the photosynthetic nutrient green algae solution is 1-5:1, wherein the number of nitrogen-fixing blue-green algae in the nitrogen-fixing blue-green algae solution is not less than 500 ten thousand per mL, and the number of photosynthetic nutrient green algae in the photosynthetic nutrient green algae solution is not less than 500 ten thousand per mL.

4. The technology for cultivating fruit trees based on nitrogen-fixing cyanobacteria and photosynthetic nutritional green algae according to claim 3, wherein the nitrogen-fixing cyanobacteria is anabaena azotoformans, and the photosynthetic nutritional green algae is chlorella pyrenoidosa.

5. The technology for cultivating fruit trees based on nitrogen-fixing cyanobacteria and photosynthetic nutritious green algae as claimed in claim 3, wherein the root mixed nutrient solution and the foliar nutrient solution are diluted by adding water.

6. The fruit tree cultivation technology based on nitrogen-fixing blue-green algae and photosynthetic nutrient green algae of claim 3, wherein the nitrogen-fixing blue-green algae solution and the photosynthetic nutrient green algae solution are obtained by culturing nitrogen-fixing blue-green algae and photosynthetic nutrient green algae in a culture medium, wherein the culture medium comprises water and the following components in concentration: 11-15mg/L C₁₀H₁₆N₂O₈、11-15mg/L C₆H₈O₇、0.11-0.15mg/L FeSO₄·7H₂O、0.11-0.15g/L NaHCO₃、0.11-0.15g/L CaCl₂、0.11-0.15g/L MgSO₄·7H₂O、0.55-0.75g/L K₂HPO₄、0.11-0.15g/L KH₂PO₄、11-15mg/L MnCl₂·4H₂O、0.11-0.15mg/L ZnSO₄·7H₂O、0.11-0.15mg/L CuSO₄·5H₂O、0.11-0.15mg/L Na₂MoO₄·2H₂O、11-15mg/L H₃BO₃11-15mg/L pinellia agglutinin protein.

7. The cultivation technique for fruit trees based on nitrogen-fixing blue-green algae and photosynthetic nutritional green algae as claimed in claim 6, wherein the pH value of the water used in the culture medium is 7.5-8.5, the TDS value is 19-21, and when the nitrogen-fixing blue-green algae/photosynthetic nutritional green algae are cultured in the culture medium, the illumination is continued and purified air is introduced, the pressure of the purified air is 0.5-0.7MPa, the illumination intensity is 2500-.

8. The cultivation technique of fruit trees based on blue-green algae with nitrogen fixation and photosynthetic nutrition as claimed in claim 7, wherein the production management of fruit trees in each growth cycle during the cultivation of fruit trees comprises the following modes:

and (4) nutritional reserve stage: cultivating and fertilizing;

a dormant period: applying root mixed nutrient solution to the roots of the fruit trees, wherein the application amount is 200-400mL per mu in terms of the protein nucleus chlorella solution;

the growth period of the new branch is as follows: disease prevention, fertilization, and application of a root mixed nutrient solution and a leaf surface nutrient solution, wherein the application amount of the root mixed nutrient solution and the leaf surface nutrient solution is 400mL per mu in terms of chlorella pyrenoidosa solution;

slow growth period of fruits: watering, fertilizing, preventing plant diseases and insect pests and applying a foliar nutrient solution, wherein the application amount of the foliar nutrient solution is 200-800mL per mu in terms of the protein chlorella solution.

9. The cultivation technique of fruit trees based on blue-green algae with nitrogen fixation and photosynthetic nutrition as claimed in claim 8, wherein the production management of fruit trees in each growth cycle during the cultivation of fruit trees comprises the following modes:

and (4) nutritional reserve stage: intertilling, weeding, removing root tillers, loosening soil, preserving soil moisture and applying fertilizer;

a dormant period: trimming and pruning, applying root mixed nutrient solution to the roots of the fruit trees along with water during watering, wherein the application amount is 200-400mL per mu in terms of protein chlorella solution;

and (3) during a recovery period: coating the tree body with water;

and (3) in the sprouting stage: clearing the garden;

the growth period of the new branch is as follows: disease prevention, fertilization, and application of a root mixed nutrient solution and a leaf surface nutrient solution, wherein the application amount of the root mixed nutrient solution and the leaf surface nutrient solution is 200-400mL per mu in terms of chlorella vulgaris solution;

and (3) flowering period: bee releasing to promote pollination;

growth period of young fruits: pruning to reduce nutrient loss;

slow growth period of fruits: watering, fertilizing, weeding, preventing plant diseases and insect pests and applying a foliar nutrient solution, wherein the application amount of the foliar nutrient solution is 200-800mL per mu in terms of the protein chlorella solution;

flower bud differentiation and fruit expansion period: watering, pruning and fertilizing.

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