CN114158575A - 一种抗氧化抑菌植物提取物的制备方法及其应用 - Google Patents

一种抗氧化抑菌植物提取物的制备方法及其应用 Download PDF

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CN114158575A
CN114158575A CN202111523746.7A CN202111523746A CN114158575A CN 114158575 A CN114158575 A CN 114158575A CN 202111523746 A CN202111523746 A CN 202111523746A CN 114158575 A CN114158575 A CN 114158575A
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倪柏雯
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Jiangsu Times Mingyang New Biotechnology Research Institute Co ltd
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Abstract

本发明属于植物提取物领域，具体公开了一种抗氧化抑菌植物提取物的制备方法及其应用，所述提取物由中华葫芦藓提取物、芦荟提取物、金露梅提取物、茶叶提取物、洋葱提取物、仙人掌提取物、蒲公英提取物、金银花提取物、紫罗兰提取物、裂壶藻破壁粉、番茄红素和鼠尾草酸这些原料复方制备组成；本发明所公开的植物提取物原料易得，纯天然绿色健康，制备方便，抗氧化能力好，抑菌能力强，可以广泛用于消毒剂或者化妆品原辅料使用。

Description

一种抗氧化抑菌植物提取物的制备方法及其应用
技术领域
本发明属于植物提取物领域，具体公开了一种抗氧化抑菌植物提取物的制备方法及其应用。
背景技术
植物提取物是以植物为原料，按照对提取的最终产品的用途的需要，经过物理化学提取分离过程，定向获取和浓集植物中的某一种或多种有效成分，而不改变其有效成分结构而形成的产品。植物提取物的应用范围如今已经非常的广泛，除了传统的用于中药产品，随着人们对天然产品的信任和依赖逐步增加，植物提取物很大一部分已经用于保健品，食品配料范畴。另外，植物提取物近年来在化妆品，饲料方面都已经有了应用。1.根据有效成分的含量可分为有效单体提取物、标准提取物和比率提取物三类；2.按成分分为甙、酸、多酚、多糖、萜类、黄酮、生物碱等；3.按照产品形态可分为植物油、浸膏、粉、晶状体等。4.按照用途，植物提取物又可分为天然色素制品类、中药提取物制品、提取物制品类和浓缩制品类。
国际上部分畅销的植物提取物品种有几个分类，比如，红景天、银杏、人参提取物等用于健脑、益智、防治老年痴呆领域；绿茶、枳实、苹果、苦瓜多肽提取物等应用于减肥，降血糖兼防治糖尿病；紫杉醇、茶多酚、茶氨酸、生物黄酮类，如番茄红素，花青素等应用于天然抗癌领域；甘草、大蒜、黄芪、大豆提取物应用于人体免疫系统领域。随着新冠疫情的发展，人们对于消毒剂等的需求也在增长，由于抗氧化抑菌植物提取物的绿色天然无污染，越来越获得人们的青睐。目前的抑菌植物提取物的种类往往局限在传统的中药抗菌植物中，急需扩大原料来源，提供消费者更多的选择。
发明内容
针对以上不足，本发明公开了一种抗氧化抑菌植物提取物的制备方法及其应用。
本发明的技术方案如下：
一种抗氧化抑菌植物提取物，所述提取物由中华葫芦藓提取物、芦荟提取物、金露梅提取物、茶叶提取物、洋葱提取物、仙人掌提取物、蒲公英提取物、金银花提取物、紫罗兰提取物、裂壶藻破壁粉、番茄红素和鼠尾草酸这些原料复方制备组成。
进一步的，上述一种抗氧化抑菌植物提取物，所述提取物原料按重量份的组成如下：中华葫芦藓提取物100份、芦荟提取物30-60份、金露梅提取物20-40 份、茶叶提取物20-40份、洋葱提取物20-40份、仙人掌提取物10-30份、蒲公英提取物10-30份、金银花提取物10-20份、紫罗兰提取物5-15份、裂壶藻破壁粉5-15份、番茄红素4-8份和鼠尾草酸4-8份。
进一步的，上述一种抗氧化抑菌植物提取物，所述提取物原料按重量份的组成如下：中华葫芦藓提取物100份、芦荟提取物45份、金露梅提取物30份、茶叶提取物30份、洋葱提取物30份、仙人掌提取物20份、蒲公英提取物20份、金银花提取物15份、紫罗兰提取物10份、裂壶藻破壁粉10份、番茄红素6份和鼠尾草酸6份。
进一步的，上述一种抗氧化抑菌植物提取物的制备方法，包括以下步骤：
(1)按重量比称取中华葫芦藓、芦荟、金露梅、茶叶、洋葱、仙人掌、蒲公英、金银花、紫罗兰，上述原料均为新鲜采摘；
(2)将上述原料放入粉碎机粉碎，过50-200目筛，随后采用酶解水提的方式进行提取，所述酶解过程使用酶的种类为：纤维素酶10000-30000U/Kg、半纤维素酶5000-15000U/Kg、果胶酶5000-15000U/Kg，酶解时间为：4-10h、酶解温度为30-40℃、酶解pH为7-9；
(3)所得提取液过D101大孔树脂柱吸附、洗脱之后浓缩、冷冻干燥成粉A；
(4)裂壶藻培养后经酶解破壁、冷冻干燥成粉B，所述酶解过程使用酶的种类为：纤维素酶20000-40000U/Kg、果胶酶1000-2000U/Kg，酶解时间为： 1-3h、酶解温度为20-30、酶解pH为5-7；
(5)将上述粉A、粉B与市售番茄红素和鼠尾草酸按比例混匀，形成抗氧化抑菌植物提取物。
进一步的，上述一种抗氧化抑菌植物提取物的制备方法，所述步骤(2)中，将上述原料放入粉碎机粉碎，过100目筛，随后采用酶解水提的方式进行提取，所述酶解过程使用酶的种类为：纤维素酶20000U/Kg、半纤维素酶10000U/Kg、果胶酶10000U/Kg，酶解时间为：7h、酶解温度为35℃、酶解pH为8。
进一步的，上述一种抗氧化抑菌植物提取物的制备方法，所述步骤(4)中，裂壶藻培养后经酶解破壁、冷冻干燥成粉B，所述酶解过程使用酶的种类为：纤维素酶30000U/Kg、果胶酶1500U/Kg，酶解时间为：2h、酶解温度为25、酶解pH为6。
进一步的，上述一种抗氧化抑菌植物提取物的制备方法，由以下步骤组成：
(1)按重量比称取中华葫芦藓、芦荟、金露梅、茶叶、洋葱、仙人掌、蒲公英、金银花、紫罗兰，上述原料均为新鲜采摘；
(2)将上述原料放入粉碎机粉碎，过100目筛，随后采用酶解水提的方式进行提取，所述酶解过程使用酶的种类为：纤维素酶20000U/Kg、半纤维素酶 10000U/Kg、果胶酶10000U/Kg，酶解时间为：7h、酶解温度为35℃、酶解pH 为8；
(3)所得提取液过D101大孔树脂柱吸附、洗脱之后浓缩、冷冻干燥成粉A；
(4)裂壶藻培养后经酶解破壁、冷冻干燥成粉B，所述酶解过程使用酶的种类为：纤维素酶30000U/Kg、果胶酶1500U/Kg，酶解时间为：2h、酶解温度为25、酶解pH为6；
(5)将上述粉A、粉B与市售番茄红素和鼠尾草酸按比例混匀，形成抗氧化抑菌植物提取物。
进一步的，上述抗氧化抑菌植物提取物在制备消毒剂或者化妆品中的应用。
根据以上技术方案可知，本发明至少有以下有益效果：
本发明公开了一种抗氧化抑菌植物提取物的制备方法及其应用，创新的添加了中华葫芦藓提取物、金露梅提取物和裂壶藻破壁粉，结合传统的抗菌中药植物提取物，精心进行调配，再加入番茄红素和鼠尾草酸，获得了一种抗氧化能力强，气味芳香，抑菌效果好的新型植物提取复合物，其原料易得，制备方法简单环保成本低，可以广泛用于消毒剂和化妆品的制备之中。
具体实施方式
下面对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其它实施例，都属于本发明保护的范围。
本发明实施例中使用的试剂或仪器未注明生产厂商者，均为可以通过市场渠道购获得的常规试剂产品。
实施例1
一种抗氧化抑菌植物提取物，提取物原料按重量份的组成如下：中华葫芦藓提取物100份、芦荟提取物30份、金露梅提取物20份、茶叶提取物20份、洋葱提取物20份、仙人掌提取物10份、蒲公英提取物10份、金银花提取物10 份、紫罗兰提取物5份、裂壶藻破壁粉5份、番茄红素4份和鼠尾草酸4份。
上述抗氧化抑菌植物提取物的制备方法包括以下步骤：
(1)按重量比称取中华葫芦藓、芦荟、金露梅、茶叶、洋葱、仙人掌、蒲公英、金银花、紫罗兰，上述原料均为新鲜采摘；
(2)将上述原料放入粉碎机粉碎，过50目筛，随后采用酶解水提的方式进行提取，所述酶解过程使用酶的种类为：纤维素酶10000U/Kg、半纤维素酶 5000U/Kg、果胶酶5000U/Kg，酶解时间为：4h、酶解温度为30℃、酶解pH为7；
(3)所得提取液过D101大孔树脂柱吸附、洗脱之后浓缩、冷冻干燥成粉A；
(4)裂壶藻培养后经酶解破壁、冷冻干燥成粉B，所述酶解过程使用酶的种类为：纤维素酶20000U/Kg、果胶酶1000U/Kg，酶解时间为：1h、酶解温度为20℃、酶解pH为5-7；
(5)将上述粉A、粉B与市售番茄红素和鼠尾草酸按比例混匀，形成抗氧化抑菌植物提取物。
实施例2
一种抗氧化抑菌植物提取物，提取物原料按重量份的组成如下：中华葫芦藓提取物100份、芦荟提取物45份、金露梅提取物30份、茶叶提取物30份、洋葱提取物30份、仙人掌提取物20份、蒲公英提取物20份、金银花提取物15 份、紫罗兰提取物10份、裂壶藻破壁粉10份、番茄红素6份和鼠尾草酸6份。
上述抗氧化抑菌植物提取物的制备方法包括以下步骤：
(1)按重量比称取中华葫芦藓、芦荟、金露梅、茶叶、洋葱、仙人掌、蒲公英、金银花、紫罗兰，上述原料均为新鲜采摘；
(2)将上述原料放入粉碎机粉碎，过100目筛，随后采用酶解水提的方式进行提取，所述酶解过程使用酶的种类为：纤维素酶20000U/Kg、半纤维素酶 10000U/Kg、果胶酶10000U/Kg，酶解时间为：7h、酶解温度为35℃、酶解pH 为8；
(3)所得提取液过D101大孔树脂柱吸附、洗脱之后浓缩、冷冻干燥成粉A；
(4)裂壶藻培养后经酶解破壁、冷冻干燥成粉B，所述酶解过程使用酶的种类为：纤维素酶30000U/Kg、果胶酶1500U/Kg，酶解时间为：2h、酶解温度为25、酶解pH为6；
(5)将上述粉A、粉B与市售番茄红素和鼠尾草酸按比例混匀，形成抗氧化抑菌植物提取物。
实施例3
一种抗氧化抑菌植物提取物，提取物原料按重量份的组成如下：中华葫芦藓提取物100份、芦荟提取物60份、金露梅提取物40份、茶叶提取物40份、洋葱提取物40份、仙人掌提取物30份、蒲公英提取物30份、金银花提取物20 份、紫罗兰提取物15份、裂壶藻破壁粉15份、番茄红素8份和鼠尾草酸8份。
上述抗氧化抑菌植物提取物的制备方法包括以下步骤：
(1)按重量比称取中华葫芦藓、芦荟、金露梅、茶叶、洋葱、仙人掌、蒲公英、金银花、紫罗兰，上述原料均为新鲜采摘；
(2)将上述原料放入粉碎机粉碎，过200目筛，随后采用酶解水提的方式进行提取，所述酶解过程使用酶的种类为：纤维素酶30000U/Kg、半纤维素酶 15000U/Kg、果胶酶15000U/Kg，酶解时间为：10h、酶解温度为40℃、酶解pH 为9；
(3)所得提取液过D101大孔树脂柱吸附、洗脱之后浓缩、冷冻干燥成粉A；
(4)裂壶藻培养后经酶解破壁、冷冻干燥成粉B，所述酶解过程使用酶的种类为：纤维素酶40000U/Kg、果胶酶2000U/Kg，酶解时间为：3h、酶解温度为30、酶解pH为7；
(5)将上述粉A、粉B与市售番茄红素和鼠尾草酸按比例混匀，形成抗氧化抑菌植物提取物。
测试例
将实施例1-3制备获得的植物提取物和市售抗氧化抑菌植物提取物进行对比测试，进行金黄色葡萄球菌和大肠杆菌的抑菌能力对比测试，测试方法为滤纸片扩散方法，抗氧化能力参照文献(Stoilova I,Krastanov A,Stoyanova A,et al.Antioxidantactivity of a ginger extract(Zingiber officinale)[J]. Food Chem,2007,102(3):764-770.)进行，测试结果如表1所示。
Figure BDA0003406743310000061
注：“+”有显著的抑制作用；“～”无显著的抑制作用；“-”无抑制作用。
由以上实施例可知：本发明公开了一种抗氧化抑菌植物提取物的制备方法及其应用，创新的添加了中华葫芦藓提取物、金露梅提取物和裂壶藻破壁粉，结合传统的抗菌中药植物提取物，精心进行调配，再加入番茄红素和鼠尾草酸，获得了一种抗氧化能力强，气味芳香，抑菌效果好的新型植物提取复合物，其原料易得，制备方法简单环保成本低，可以广泛用于消毒剂和化妆品的制备之中
以上仅为本发明的较佳实施例而已，不能以此限定本发明的保护范围，即大凡依本发明权利要求书及发明内容所做的简单的等效变化与修改，皆仍属于本发明专利申请的保护范围。

Claims (8)

1.一种抗氧化抑菌植物提取物，其特征在于，所述提取物由中华葫芦藓提取物、芦荟提取物、金露梅提取物、茶叶提取物、洋葱提取物、仙人掌提取物、蒲公英提取物、金银花提取物、紫罗兰提取物、裂壶藻破壁粉、番茄红素和鼠尾草酸这些原料复方制备组成。
2.根据权利要求1所述的一种抗氧化抑菌植物提取物，其特征在于，提取物原料按重量份的组成如下：中华葫芦藓提取物100份、芦荟提取物30-60份、金露梅提取物20-40份、茶叶提取物20-40份、洋葱提取物20-40份、仙人掌提取物10-30份、蒲公英提取物10-30份、金银花提取物10-20份、紫罗兰提取物5-15份、裂壶藻破壁粉5-15份、番茄红素4-8份和鼠尾草酸4-8份。
3.根据权利要求1所述的一种抗氧化抑菌植物提取物，其特征在于，提取物原料按重量份的组成如下：中华葫芦藓提取物100份、芦荟提取物45份、金露梅提取物30份、茶叶提取物30份、洋葱提取物30份、仙人掌提取物20份、蒲公英提取物20份、金银花提取物15份、紫罗兰提取物10份、裂壶藻破壁粉10份、番茄红素6份和鼠尾草酸6份。
4.如权利要求1-3任一项所述的抗氧化抑菌植物提取物的制备方法，其特征在于，包括以下步骤：
（1）按重量比称取中华葫芦藓、芦荟、金露梅、茶叶、洋葱、仙人掌、蒲公英、金银花、紫罗兰，上述原料均为新鲜采摘；
（2）将上述原料放入粉碎机粉碎，过50-200目筛，随后采用酶解水提的方式进行提取，所述酶解过程使用酶的种类为：纤维素酶10000-30000U/Kg、半纤维素酶5000-15000U/Kg、果胶酶5000-15000U/Kg，酶解时间为：4-10h、酶解温度为30-40℃、酶解pH为7-9；
（3）所得提取液过D101大孔树脂柱吸附、洗脱之后浓缩、冷冻干燥成粉A；
（4）裂壶藻培养后经酶解破壁、冷冻干燥成粉B，所述酶解过程使用酶的种类为：纤维素酶20000-40000 U/Kg、果胶酶1000-2000 U/Kg，酶解时间为：1-3h、酶解温度为20-30、酶解pH为5-7；
（5）将上述粉A、粉B与市售番茄红素和鼠尾草酸按比例混匀，形成抗氧化抑菌植物提取物。
5.根据权利要求4所述的抗氧化抑菌植物提取物的制备方法，其特征在于，所述步骤（2）中，将上述原料放入粉碎机粉碎，过100目筛，随后采用酶解水提的方式进行提取，所述酶解过程使用酶的种类为：纤维素酶20000U/Kg、半纤维素酶10000U/Kg、果胶酶10000U/Kg，酶解时间为：7h、酶解温度为35℃、酶解pH为8。
6.根据权利要求4所述的抗氧化抑菌植物提取物的制备方法，其特征在于，所述步骤（4）中，裂壶藻培养后经酶解破壁、冷冻干燥成粉B，所述酶解过程使用酶的种类为：纤维素酶30000 U/Kg、果胶酶1500 U/Kg，酶解时间为：2h、酶解温度为25、酶解pH为6。
7.根据权利要求4所述的抗氧化抑菌植物提取物的制备方法，其特征在于，有以下步骤组成：
（1）按重量比称取中华葫芦藓、芦荟、金露梅、茶叶、洋葱、仙人掌、蒲公英、金银花、紫罗兰，上述原料均为新鲜采摘；
（2）将上述原料放入粉碎机粉碎，过100目筛，随后采用酶解水提的方式进行提取，所述酶解过程使用酶的种类为：纤维素酶20000U/Kg、半纤维素酶10000U/Kg、果胶酶10000U/Kg，酶解时间为：7h、酶解温度为35℃、酶解pH为8；
（3）所得提取液过D101大孔树脂柱吸附、洗脱之后浓缩、冷冻干燥成粉A；
（4）裂壶藻培养后经酶解破壁、冷冻干燥成粉B，所述酶解过程使用酶的种类为：纤维素酶30000 U/Kg、果胶酶1500 U/Kg，酶解时间为：2h、酶解温度为25、酶解pH为6；
（5）将上述粉A、粉B与市售番茄红素和鼠尾草酸按比例混匀，形成抗氧化抑菌植物提取物。
8.如权利要求1-3任一项所述的抗氧化抑菌植物提取物在制备消毒剂或者化妆品中的应用。
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