CN114146150A - Pharmaceutical composition for treating scutcheon paralichthys maximus scuticociliasis and application thereof - Google Patents
Pharmaceutical composition for treating scutcheon paralichthys maximus scuticociliasis and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/20—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing sulfur, e.g. dimethyl sulfoxide [DMSO], docusate, sodium lauryl sulfate or aminosulfonic acids
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
- A61K9/0017—Non-human animal skin, e.g. pour-on, spot-on
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
- A61K9/0056—Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention provides a pharmaceutical composition for treating turbot sculellariasis and application thereof, belonging to the field of medicines, wherein the active ingredients of the pharmaceutical composition comprise a turmeric extract and fatty alcohol-polyoxyethylene ether sodium sulfate, the turmeric extract is an extract of a turmeric medicinal material, and the weight ratio of the turmeric medicinal material to the fatty alcohol-polyoxyethylene ether sodium sulfate is (100-400): 4, optionally 200: 4. 100:4 or 400: 4. In vitro insecticidal experiments and animal infection experiments prove that the addition of turmeric and sodium fatty alcohol polyoxyethylene ether sulfate (AES) can kill the scuticociliatida, obviously reduce the infection rate and the death rate of the scuticocitida scophthalmus and have better scuticocitida resistance effect. After the turmeric and the fatty alcohol polyoxyethylene ether sodium sulfate (AES) are used together, the insecticidal effect is obviously improved. The combination of the ethanol extract of turmeric and sodium Alcohol Ether Sulfate (AES) is found for the first time to enhance the effect of killing the scuticociliatus pellis.
Description
Technical Field
The invention relates to the technical field of medicines, and particularly relates to a pharmaceutical composition for treating turbot paralichthys olivaceus scuticociliatosis and application thereof.
Background
Turbot (Scophthalmus maximus), also known as turbot, is naturally distributed on the european coast from the east to the northeast of the atlantic ocean for life at the bottom of the camps. Since the introduction of China, good economic benefits are created, and the artificial culture of the marine aquaculture method becomes one of the important supporting industries of China. However, with the increasing culture density of turbots and the lagged research on disease control, various culture diseases are coming to the fore. Among the various breeding diseases, parasitosis caused by scuticociliasis, cryptocaryon, trichodina and flagellates causes great loss to the turbot breeding industry, and among them, scuticociliasis, which is the most harmful, is a rapidly fatal systemic infection.
Scophthalmus maximus parasitized sculus paralichthys (Paralembus digitormis) is crab-inhabiting ectoparasite, belongs to the dirophilic family, and is bred by bisection, the caused Scophthalmus maximus disease has the characteristics of strong infectivity and great harm, and especially, huge loss can be caused to farmers once seedlings are erupted. The pathogeny is mainly crab-inhabiting Aralia elata and can enter a culture system through various ways to cause the disease attack. The pelciliates are abundantly present in the affected part, body surface and gill of turbot, and are also abundantly present in liver, spleen, kidney, brain and heart, and also abundantly present in ascites. The body surface of the diseased fish at the initial infection stage has leukoplakia and mucus increase, the tissues at the focus of infection appear red and swollen along with the development of the disease, and the fish has soft and edema feeling, and the skin of the body of the fish can also have the focus of infection until ulceration and hemorrhage. The diseased fish generally becomes dark in body color, the vitality is weakened, the food intake is reduced, and the growth is slowed down; when the group is scattered in the culture pond, the phenomenon of rotation and swimming often occurs. Ciliates can infect most internal organs including brain tissue.
At present, few effective methods for treating diseases of scuticociliatida are used in China, in order to prevent the diseases in actual production, antibiotics and chemical disinfectants are often used for treating the diseases of fish bodies, but because a culture manufacturer lacks health culture consciousness, the antibiotics and the chemical disinfectants are used repeatedly in large blind dosage, even a plurality of forbidden drugs are frequently used, so that a large amount of drug residues appear on the fish bodies, and the serious potential safety hazard exists on aquatic products. Aiming at the frequent occurrence of ciliate disease, formaldehyde, copper sulfate and the like are mainly adopted to prevent and treat the ciliate disease in China, and even farmers use prohibited pesticides to treat ciliate diseases. However, due to the fact that the turbot is extremely easy to be poisoned and die by using pesticides, and the scuticociliatosis is treated by using the pesticides and chemical drugs for a long time, the marine environment is polluted, and the problem becomes a green trade barrier problem of the current aquatic product export, so that the search for novel, safe and environment-friendly insecticide drugs becomes urgent.
Sodium fatty alcohol polyoxyethylene ether sulphate (AES) is a colorless, white or pale yellow viscous liquid, and the odor is the alkaline anionic surfactant with the typical soap smell. Is easy to dissolve in water, has excellent decontamination, emulsification, foaming performance and hard water resistance, and is widely applied to washing cosmetics such as shampoo, bath lotion, tableware detergent, compound soap and the like; it can be used as wetting agent and cleaning agent in textile industry.
Curcuma longa L, as well as Curcuma longa L, is pungent, bitter and warm in nature. It enters spleen and liver meridians. The functions of breaking blood and promoting qi circulation, dissipating nodulation and relieving pain. The main treatment is as follows: qi stagnation and blood stasis, chest and abdomen pain, traumatic swelling and pain, and wind-damp arthralgia. At present, although research proves that the turmeric extract has certain insecticidal and bactericidal activity, the effect of turmeric on resisting Scophthalmus maximus ciliates is not reported.
Disclosure of Invention
Object of the Invention
The invention aims to provide a drug composition for treating turbot sculellariatosis with low toxicity and environmental friendliness and application thereof, which can reduce the pollution of formaldehyde, copper sulfate and prohibited pesticides discharged in the turbot culture process for treating sculelitis to the ocean.
Solution scheme
In order to realize the purpose of the invention, the technical scheme adopted by the invention is as follows:
in one aspect, the invention provides a pharmaceutical composition for treating sculellaria maximowiczii ciliate disease, wherein the active ingredients of the pharmaceutical composition comprise turmeric extract and fatty alcohol-polyoxyethylene ether sodium sulfate.
Further, the turmeric extract is an extract of a turmeric medicinal material, and the weight ratio of the turmeric medicinal material to the fatty alcohol-polyoxyethylene ether sodium sulfate is (100-400): 4, optionally 200:4, 100:4 or 400: 4.
Further, the turmeric extract is an alcoholic extract of turmeric, optionally an alcoholic extract.
Further, the preparation method of the turmeric extract comprises the following steps: pulverizing Curcuma rhizome, adding food-grade 95% ethanol, soaking, extracting, concentrating to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount of multiple times per 200g Curcuma rhizome, soaking, extracting, concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount of 10 times per 200g Curcuma rhizome after pulverizing, soaking, extracting, and concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution.
In another aspect, there is provided a method for preparing the pharmaceutical composition for treating scutcheon paralichthys olivaceus ciliophora disease, wherein the fatty alcohol-polyoxyethylene ether sodium sulfate is added to the turmeric extract, and the mixture is uniformly mixed, optionally, the turmeric extract is an alcohol extract of the turmeric, optionally, the turmeric extract is prepared by: pulverizing Curcuma rhizome, adding food-grade 95% ethanol, soaking, extracting, concentrating to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount of multiple times per 200g Curcuma rhizome, soaking, extracting, concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount of 10 times per 200g Curcuma rhizome after pulverizing, soaking, extracting, and concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution.
In still another aspect, a method for using the pharmaceutical composition for treating the sculellaria maximus is provided, the pharmaceutical composition is splashed into seawater, and medicated bath is carried out on the sculellaria maximus suffering from sculellaria maximus.
Further, the medicated bath method comprises the following steps: the medicated bath is carried out for 30 minutes every day and is continued for 5-7 days.
On the other hand, the application of the pharmaceutical composition in preparing the anti-scuticociliatida medicine or the feed additive is provided, the pharmaceutical composition comprises a turmeric extract and fatty alcohol-polyoxyethylene ether sodium sulfate, and the turmeric extract is an extract of a turmeric medicinal material.
Further, the turmeric extract is an extract of a turmeric medicinal material, and the weight ratio of the turmeric medicinal material to the fatty alcohol-polyoxyethylene ether sodium sulfate is (100-400): 4, optionally 200: 4. 100:4 or 400: 4.
Further, the turmeric extract is an alcohol extract of turmeric; optionally, the preparation method of the turmeric extract comprises: pulverizing Curcuma rhizome, adding food-grade 95% ethanol, soaking, extracting, concentrating to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount of multiple times per 200g Curcuma rhizome, soaking, extracting, concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount of 10 times per 200g Curcuma rhizome after pulverizing, soaking, extracting, and concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution.
Further, 1L of the pharmaceutical composition is sprinkled in sea water per 8000kg for treating Scophthalmus maximus suffering from scuticociliatosis, optionally, the treatment method adopts medicated bath, optionally, 30 minutes daily, and continuously for 5-7 days.
The inventor takes 100-400 g of turmeric, after ethanol extraction, 4g of fatty alcohol-polyoxyethylene ether sodium sulfate (AES) is added into an extracting solution, the mixture is uniformly mixed, and the mixed solution is splashed into 1000kg of water for medicated bath of the diseased turbot.
Advantageous effects
In vitro insecticidal experiments and animal infection experiments prove that the addition of turmeric and sodium fatty alcohol polyoxyethylene ether sulfate (AES) can kill the scuticociliatida, obviously reduce the infection rate and the death rate of the scuticocitida scophthalmus and have better scuticocitida resistance effect. After the turmeric and the fatty alcohol polyoxyethylene ether sodium sulfate (AES) are used together, the insecticidal effect is obviously improved. The combination of the ethanol extract of turmeric and sodium Alcohol Ether Sulfate (AES) is found for the first time to enhance the effect of killing the scuticociliatus pellis.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Furthermore, in the following detailed description, numerous specific details are set forth in order to provide a better understanding of the present invention. It will be understood by those skilled in the art that the present invention may be practiced without some of these specific details. In some embodiments, materials, elements, methods, means, and the like that are well known to those skilled in the art are not described in detail in order to not unnecessarily obscure the present invention.
Throughout the specification and claims, unless explicitly stated otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or component but not the exclusion of any other element or component.
Example 1
Preparation of sodium fatty Alcohol Ether Sulfate (AES) liquid formulation: and adding 4g of fatty alcohol-polyoxyethylene ether sodium sulfate (AES) into 200mL of food-grade 95% ethanol, and uniformly mixing to obtain the composition.
The usage and dosage of the example 1 are as follows: 1L of the extract of example 1 is sprinkled into 2000kg of seawater, and medicated bath for Scophthalmus maximus suffering from scuticociliatosis is carried out for 30 minutes per day and continuously for 5-7 days.
Example 2
Preparing a turmeric extracting solution: pulverizing 200g Curcuma rhizome, adding 10 times of food-grade 95% ethanol, soaking and extracting for 24-48 h by shaking table, and concentrating the extractive solution to 200 mL.
The usage and dosage of the example 2 are as follows: taking 1L of the extract of the embodiment 2, sprinkling the extract into 1000kg of seawater, and carrying out medicated bath on turbot suffering from scuticociliatosis for 30 minutes every day and continuously carrying out the medicated bath for 5-7 days.
Example 3
Preparation of turmeric extract and sodium fatty alcohol polyoxyethylene ether sulfate (AES) formulation: pulverizing 200g of Curcuma rhizome, extracting with 10 times of food-grade 95% ethanol, soaking and extracting for 24-48 h by shaking table, concentrating the extractive solution to 200mL, and adding 4g of sodium fatty alcohol polyoxyethylene ether sulfate (AES).
The usage and dosage of the example 3: taking 1L of the extract of example 3, sprinkling into 8000kg of seawater, and carrying out medicated bath for Scophthalmus maximus suffering from scuticociliatosis for 30 minutes per day, and continuously carrying out medicated bath for 5-7 days.
Example 4
Preparing a turmeric extracting solution: taking 100g of turmeric, crushing, adding 10 times of food-grade 95% ethanol, shaking a table, soaking and extracting for 24-48 h, and concentrating the extracting solution to 200 mL.
Example 5
Preparing a turmeric extracting solution: taking 400g of turmeric, crushing, adding 10 times of food-grade 95% ethanol, shaking a table, immersing and extracting for 24-48 h, and concentrating the extracting solution to 200 mL.
Example 6
Preparation of turmeric extract and sodium fatty alcohol polyoxyethylene ether sulfate (AES) formulation: taking 100g of turmeric, crushing, adding 10 times of food-grade 95% ethanol for extraction, shaking and soaking in a shaking table for 24-48 h, concentrating the extract to 200mL, and adding 4g of fatty alcohol-polyoxyethylene ether sodium sulfate (AES).
Example 7
Preparation of turmeric extract and sodium fatty alcohol polyoxyethylene ether sulfate (AES) formulation: taking 400g of turmeric, crushing, adding 10 times of food-grade 95% ethanol for extraction, shaking and soaking in a shaking table for 24-48 h, concentrating the extract to 200mL, and adding 4g of fatty alcohol-polyoxyethylene ether sodium sulfate (AES).
The combined insect-resistant concentration index detection method comprises the following steps: reference to the calculation formula of Fractional Inhibition Concentration (FIC): FIC index is MIC (combination a)/MIC (combination a alone) + MIC (combination B)/MIC (combination B alone). FIC index interpretation criteria: when the FIC index is less than 0.5, the two medicines act synergistically; when the FIC index is 0.5-1, the two medicines have an additive effect; when the FIC index is more than l and less than 2, the two medicines have unrelated effects; when the FIC index is greater than 2, the two drugs are antagonistic.
The effect of examples 1, 2, 3 in killing scuticociliatus ciliates is illustrated below in connection with in vitro insecticidal tests.
In vitro insecticidal test: taking a 96-deep-hole plate, firstly adding 0.6mL of seawater into each hole, then adding 0.6mL of the solution obtained in the step 1, diluting by times, and diluting to a 12 th hole, wherein the concentration of the medicines is 1/2-1/4096 respectively; according to the method, the samples of example 2, 3 and 95% ethanol are diluted in sequence, 120 mu L of diluted sample is absorbed and added into a 96-hole cell culture plate, then 120 mu L of ciliate solution is added into each hole, the concentration of each hole is 1/4-1/8192, the cell culture plate is slightly shaken for 1 minute and then kept still for 2 hours, and whether ciliates die or not is observed by an inverted microscope, and the drug effect is judged. Meanwhile, a blank control group is set, and the concentration of the ciliate peltate is the same as that of the test group. The results show that: the minimum insecticidal concentration of the 95% ethanol solvent control group was 1:8, the minimum in vitro insecticidal concentration of example 1 was 1:2048, the minimum in vitro insecticidal concentration of example 2 was 1:1024, and the minimum in vitro insecticidal concentration of example 3 was 1: 8192. Through in vitro insecticidal test, the combination of turmeric and fatty alcohol-polyoxyethylene ether sodium sulfate (AES) can obviously improve the insecticidal effect, the same insecticidal effect, the minimum use concentration of the turmeric extract and the fatty alcohol-polyoxyethylene ether sodium sulfate (AES) after combination is 25% of that of the turmeric extract alone and 12.5% of that of the fatty alcohol-polyoxyethylene ether sodium sulfate (AES) alone, and the use amounts of the two materials can be obviously reduced. Meanwhile, the FIC index of the combined insecticide of example 3 is 3/8 and is less than 0.5, which indicates that the turmeric extract and sodium fatty alcohol polyoxyethylene ether sulfate (AES) have synergistic effect.
The effect of examples 1, 4, 5, 6, 7 on the killing of scuticociliatus ciliates is illustrated below in connection with in vitro insecticidal tests.
In vitro insecticidal test: adding 1mL of the volume of the solution in the example 1 into a 100mL measuring flask, adding seawater to the scale, uniformly mixing to prepare a 1:100 solution, and diluting by times by taking the concentration as a reference to obtain 1:200, 1:400, 1:600, 1:800, 1:1000 and 1:1200.. to 1:10000 series of concentrations; according to the method, the samples of examples 4, 5, 6, 7 and 95% ethanol are diluted in sequence, 120 mu L of each diluted sample is absorbed and added into a 96-hole cell culture plate, then 120 mu L of ciliate solution is added into each hole, the cell culture plate is slightly shaken for 1 minute and then kept still for 2 hours, and whether ciliates die or not is observed by an inverted microscope, so that the drug effect is judged. Meanwhile, a blank control group is set, and the concentration of the ciliate peltate is the same as that of the test group. The results show that: the 95% ethanol solvent control group had no effect on ciliates survival, the minimum in vitro insecticidal concentration for example 1 was 1: 2000; the minimum in vitro insecticidal concentration of example 4 is 1:600, the minimum in vitro insecticidal concentration of example 5 is 1:2000, and the minimum in vitro insecticidal concentration of example 6 is 1: 6800; the minimum in vitro insecticidal concentration for example 7 was 1: 9400. Through in vitro insecticidal test, the turmeric and fatty alcohol-polyoxyethylene ether sodium sulfate (AES) are jointly used, so that the insecticidal effect can be obviously improved, the same insecticidal effect is achieved, the use concentration of the turmeric extract and the fatty alcohol-polyoxyethylene ether sodium sulfate (AES) after being jointly used is reduced, and the use amount of two materials can be obviously reduced. Meanwhile, the combined insecticidal FIC indexes of the embodiments 6 and 7 are 13/34 and 10/47 which are both less than 0.5, which indicates that the turmeric extract and sodium fatty alcohol polyoxyethylene ether sulfate (AES) have synergistic effect.
The drug effects of the embodiments 1, 2 and 3 on preventing and treating sculellaria maximus paralichthys olivaceus ciliate disease are observed.
The peltate ciliate resistance test for turmeric is described below in conjunction with specific tests.
Constructing a turbot virus attacking model: the larva of scuticociliatus gibbosa in exponential growth phase is used for counteracting toxic substance. Rubbing 2cm × 2cm abrasion area with iron gauze on back muscle of turbot (weight about 200g), adding 50L water for fish culture at 50000/L, and oxygenating at 12 deg.C to normally feed turbot. The change of the ulcer part is observed every day, mucus or tissue is scraped off from the wound part lightly, the picture is observed and photographed under a microscope and counted, and the turbot scurvy ciliate disease is established 2 days after infection.
And (3) pharmacodynamic test: dividing into 5 groups, each group has 20 tails, and each group is provided with parallel groups, wherein the first group is a model control group: disease model groups without any drug treatment; the second group is the test group of example 1, which is challenged and added with example 1 in the feed and fed every day; the third and fourth groups were tested by taking medicated bath according to examples 2 and 3, respectively, and the dosage of the medicated bath used in examples 1, 2 and 3 was 1L of the extract sprayed into 1000kg of seawater (see Table 1), and the Scophthalmus maximus suffering from scurf ciliophora was medicated bath for 30 minutes per day, and the fifth group was a healthy control group. Test results the affected parts of the test group and the control group were gently scraped by an inoculating loop, and the activity and the number of ciliates were observed under a microscope. The test was carried out for 7 days, and the infection rate was (number of infections + number of deaths)/20 × 100%, and the mortality rate was (number of deaths/20 × 100%). The results are shown in Table 1.
TABLE 1 Effect of the examples against ciliate peltate disease
The test result shows that the effect of the embodiment 3 is obviously better than that of the embodiments 1 and 2, and the effect is obviously enhanced after the combination of the turmeric alcohol extract and the fatty alcohol-polyoxyethylene ether sodium sulfate (AES). The turmeric alcohol extract and the fatty alcohol-polyoxyethylene ether sodium sulfate (AES) have obvious curative effects on preventing and treating turbot scuticociliatosis, can reduce the death rate and infection rate of the turbot scuticociliatosis, are safer compared with chemical medicines such as formaldehyde, copper sulfate and the like, ensure food safety, do not cause obvious influence on the feeding of fish, do not influence the growth of the turbot, and can reduce the economic loss of farmers. Meanwhile, the use of chemical drugs such as formaldehyde, copper sulfate and the like is reduced, and the method has far-reaching significance for marine environmental protection.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. A pharmaceutical composition for treating sculellaria maximus sculellaria sinensis is characterized in that active ingredients of the pharmaceutical composition comprise turmeric extract and fatty alcohol-polyoxyethylene ether sodium sulfate.
2. The pharmaceutical composition for treating scutcheon paralichthys maximus ciliophora according to claim 1, wherein the turmeric extract is an extract of a turmeric medicinal material, and the weight ratio of the turmeric medicinal material to the fatty alcohol-polyoxyethylene ether sodium sulfate is (100-400): 4, optionally 200: 4. 100:4 or 400: 4.
3. The pharmaceutical composition for the treatment of scutcheon paralichthys maximus ciliophora according to claim 1 or 2, wherein the turmeric extract is an alcoholic extract, optionally an alcoholic extract, of turmeric.
4. The pharmaceutical composition for treating paralichthys maximus paralembus ciliophora according to claim 3, wherein the preparation method of the turmeric extract comprises the following steps: pulverizing Curcuma rhizome, adding food-grade 95% ethanol, soaking, extracting, concentrating to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount of multiple times per 200g Curcuma rhizome, soaking, extracting, concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount of 10 times per 200g Curcuma rhizome after pulverizing, soaking, extracting, and concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution.
5. A method for preparing the pharmaceutical composition for treating scutcheon disease as claimed in any one of claims 1 to 4, wherein the fatty alcohol-polyoxyethylene ether sodium sulfate is added to the turmeric extract, optionally the turmeric extract is an alcoholic extract of turmeric, optionally the turmeric extract is prepared by: pulverizing Curcuma rhizome, adding food-grade 95% ethanol, soaking, extracting, concentrating to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount which is multiple times of that of each 200g Curcuma rhizome after pulverizing, soaking, extracting, concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount which is 10 times of that of each 200g Curcuma rhizome after pulverizing, soaking, extracting, and concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution.
6. A method of using the pharmaceutical composition for the treatment of Scophthalmus maximus paralichthys according to any one of claims 1 to 4, wherein the pharmaceutical composition is sprinkled into seawater and the Scophthalmus maximus suffering from scurvilia disease is medicated bathed.
7. The use method according to claim 6, wherein the medicated bath method comprises: the medicated bath is carried out for 30 minutes every day and is continued for 5-7 days.
8. The application of a pharmaceutical composition in preparing an anti-scuticociliatida medicament or a feed additive is characterized in that the pharmaceutical composition comprises a turmeric extract and fatty alcohol-polyoxyethylene ether sodium sulfate, wherein the turmeric extract is an extract of a turmeric medicinal material.
9. The application of claim 8, wherein the turmeric extract is an extract of turmeric, and the weight ratio of the turmeric to sodium fatty alcohol polyoxyethylene ether sulfate is (100-400): 4, optionally 200: 4. 100:4 or 400: 4;
and/or the turmeric extract is an alcohol extract of a turmeric medicinal material; optionally, the preparation method of the turmeric extract comprises: pulverizing Curcuma rhizome, adding food-grade 95% ethanol, soaking, extracting, concentrating to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount of multiple times per 200g Curcuma rhizome, soaking, extracting, concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution, optionally adding food-grade 95% ethanol in an amount of 10 times per 200g Curcuma rhizome after pulverizing, soaking, extracting, and concentrating to 200mL to obtain Curcuma rhizome ethanol extractive solution.
10. Use according to claim 9, characterized in that 1L of the pharmaceutical composition is sprinkled per 8000kg of seawater for the treatment of turbot suffering from scuticociliatosis, optionally using a medicated bath, optionally 30 minutes daily for 5-7 days.
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| CN105943524A (en) * | 2016-05-16 | 2016-09-21 | 暨南大学 | Application of curcumin to preparation of drug for killing fish epizoic infusorians |
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| CN111603493A (en) * | 2020-06-17 | 2020-09-01 | 北京生泰尔科技股份有限公司 | Medicinal composition for resisting scuticociliatida and preparation method thereof |
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| CN105943524A (en) * | 2016-05-16 | 2016-09-21 | 暨南大学 | Application of curcumin to preparation of drug for killing fish epizoic infusorians |
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| CN111603493A (en) * | 2020-06-17 | 2020-09-01 | 北京生泰尔科技股份有限公司 | Medicinal composition for resisting scuticociliatida and preparation method thereof |
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