CN111603493A

CN111603493A - Medicinal composition for resisting scuticociliatida and preparation method thereof

Info

Publication number: CN111603493A
Application number: CN202010550999.2A
Authority: CN
Inventors: 李福元; 韩俊国; 严永; 郭智伟; 程安达; 王心喜; 王秀敏
Original assignee: Beijing Centre Biology Co ltd
Current assignee: Beijing Centre Biology Co ltd
Priority date: 2020-06-17
Filing date: 2020-06-17
Publication date: 2020-09-01
Anticipated expiration: 2040-06-17
Also published as: CN111603493B

Abstract

The invention provides a medicinal composition for resisting scuticociliatida and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 2-3 parts of sundew, 20-30 parts of ethanol with the food-grade concentration of 95% and 0.3-0.6 part of fatty alcohol-polyoxyethylene ether sodium sulfate, wherein the preparation method comprises the following steps: taking 2-3 parts of sundew according to the weight part, crushing, putting into the upper part of a reaction kettle, adding 20-30 parts of 95% food-grade ethanol, and stirring and extracting the raw materials for 5 hours to obtain an extracting solution; adding 0.3-0.6 part of fatty alcohol-polyoxyethylene ether sodium sulfate into the extracting solution extracted by S1, and uniformly mixing to obtain a mixed solution; adding 1000 parts of turbot feed into the lower part of the reaction kettle, mixing the sundew slag extracted by the S1 with the turbot feed, and fully stirring for 0.5 h. The invention has the advantages of low toxicity to turbot, environmental protection, effective reduction of infection rate of scuticociliatida ciliates and simultaneous production of extracting solution and mixed feed.

Description

Medicinal composition for resisting scuticociliatida and preparation method thereof

Technical Field

The invention relates to the technical field of anti-scuticociliatida pharmaceutical compositions, in particular to an anti-scuticocitida pharmaceutical composition and a preparation method thereof.

Background

Turbot, also known as turbot, is naturally distributed on the european coast from the east to the northeast of the atlantic ocean for life of camping on the bottom of the river. Since the introduction of China, good economic benefits are created, and the artificial culture of the marine aquaculture method becomes one of the important supporting industries of China. However, with the increasing culture density of turbots and the lagged research on disease control, various culture diseases are coming to the fore. Among various culture diseases, the scuticociliatosis, the cryptocaryon, the trichodina and the flagellates cause the parasitic diseases to cause great loss to the turbot culture industry, the scuticociliasis is the most harmful, belongs to the fast fatal systemic infection, and the turbot disease caused by the scuticociliasis has the characteristics of strong infectivity and great harm, and particularly causes great loss to farmers once seedlings are exploded. The pathogeny is mainly crab-inhabiting Aralia elata and can enter a culture system through various ways to cause the disease attack.

At present, few effective methods for treating diseases of scuticociliatida are used in China, in order to prevent the diseases in actual production, antibiotics and chemical disinfectants are often used for treating the diseases of fish bodies, but because a culture manufacturer lacks health culture consciousness, the antibiotics and the chemical disinfectants are used repeatedly in large blind dosage, even a plurality of forbidden drugs are frequently used, so that a large amount of drug residues appear on the fish bodies, and the serious potential safety hazard exists on aquatic products. Aiming at the frequent occurrence of ciliate disease, formaldehyde, copper sulfate and the like are mainly adopted to prevent and treat the ciliate disease in China, and even farmers use prohibited pesticides to treat ciliate diseases. However, the long-term use of pesticides and chemical drugs for treating scuticociliatosis not only pollutes the marine environment, but also becomes a green trade barrier problem for the current aquatic product export, so that the search for novel, safe and environment-friendly pesticides becomes an urgent task.

Therefore, there is a need to provide a new pharmaceutical composition against ciliate peltate and a preparation method thereof to solve the above technical problems.

Disclosure of Invention

The invention provides a medicinal composition for killing scuticociliatida, which has obvious effect of killing scuticociliatida, effectively reduces the infection rate of scuticocitida and can produce extracting solution and mixed feed simultaneously, and a preparation method thereof.

An anti-scuticociliatida pharmaceutical composition is characterized by being prepared from the following raw materials in parts by weight: 2-3 parts of sundew, 20-30 parts of 95% food-grade ethanol and 0.3-0.6 part of fatty alcohol-polyoxyethylene ether sodium sulfate.

A method for producing an anti-scuticociliatida pharmaceutical composition, comprising the steps of:

s1: taking 2-3 parts of sundew according to the parts by weight, crushing, putting into the upper part of a reaction kettle (100), adding 20-30 parts of 95% food-grade ethanol, and stirring and extracting the raw materials for 5 hours to obtain an extracting solution;

s2, adding 1000 parts of turbot feed into the lower part of a reaction kettle (100), mixing the sundew slag extracted in the step S1 with 1000 times of turbot feed in the step S800-;

s3: adding 0.3-0.6 part of fatty alcohol-polyoxyethylene ether sodium sulfate into the extracting solution extracted by S1, and uniformly mixing to obtain a mixed solution for resisting scuticocida maxima in medicinal bath of turbots;

wherein,

the reaction kettle in the above includes:

a mixing tank; a support structure mounted to a bottom end of the mixing tank; the power structure is mounted on the top surface of the mixing tank and comprises a hydraulic cylinder, a motor, a telescopic cylinder, a telescopic rod and a pressing plate, the hydraulic cylinder is mounted on the top surface of the mixing tank, the motor is mounted on the top surface of the hydraulic cylinder and is rotatably connected with the hydraulic cylinder, the telescopic cylinder is mounted on the bottom surface of the hydraulic cylinder, the telescopic rod is slidably connected inside the telescopic cylinder, and the top surface of the pressing plate is fixed at the bottom end of the telescopic rod; the feeding structure is arranged on the top surface of the mixing tank; the feeding structure is fixed on the inner wall of the mixing tank and comprises a partition plate, an outer cylinder, an inner cylinder and a filter screen, the side wall of the partition plate is fixed on the inner wall of the mixing tank, the outer cylinder penetrates through the partition plate, the outer wall of the inner cylinder is in sliding connection with the inner wall of the outer cylinder, and the filter screen is arranged on the upper half part of the inner cylinder; the discharging structure is rotatably connected with the inner barrel and comprises a supporting plate, a discharging door, a first wheel disc, a gear, a second wheel disc, a driving belt, a sliding barrel, a spring and a torsion spring, the supporting plate is rotatably connected with the inner barrel, the two discharging doors are rotatably connected with two sides of the supporting plate, the first wheel disc is respectively fixed on two side walls of the discharging door, the supporting plate and the discharging door are rotatably connected through the first wheel disc, the gear is rotatably connected with the bottom end of the supporting plate, the second wheel disc is fixed on the side surface of the gear, the driving belt is connected with the first wheel disc and the second wheel disc, the sliding barrel is fixed on the bottom surface of the supporting plate, the spring is mounted in the sliding barrel, and the torsion spring is sleeved on the surface of the first wheel disc, one end of the torsion spring is fixed inside the supporting plate, and the other end of the torsion spring is fixed inside the discharge door; the linkage structure is fixed on the inner bottom surface of the mixing tank and comprises a cylinder, a rotating wheel, support rods, connecting teeth and racks, the bottom end of the cylinder is fixed on the inner bottom surface of the mixing tank, the sliding cylinder is connected inside the cylinder in a sliding mode, the bottom end of the spring abuts against the top surface of the cylinder, the rotating cylinder is connected to the outer wall of the cylinder in a rotating mode, the rotating wheel is installed at the bottom end of the rotating cylinder and connected to the inner bottom surface of the mixing tank in a rotating mode, one end of each support rod is fixed to the top end of the rotating cylinder in an equiangular mode, the two connecting teeth are fixed to the other end of each support rod and the bottom end of the inner cylinder respectively, the two connecting teeth are meshed with each other, and the two racks are fixed to the top end of; and the stirring structure is respectively fixed on the outer wall of the telescopic cylinder and the outer wall of the rotary cylinder.

Preferably, the supporting structure comprises a supporting frame and a discharge door, the supporting frame is installed at the bottom end of the mixing tank, and the discharge door is rotatably connected with the side wall of the mixing tank.

Preferably, the power structure still includes material feeding mouth, puddler, slider, electrically conductive dish and wiping line, material feeding mouth is located the top surface of blending tank, a plurality of the puddler is fixed in the bottom surface of clamp plate, two the slider symmetry is fixed in the lateral wall of clamp plate, electrically conductive dish install in the top surface of pneumatic cylinder, the wiping line install in the inside of motor, just the wiping line with electrically conductive between the dish electric connection.

Preferably, feed structure includes a feed gate, an outer feeding section of thick bamboo, an interior feeding section of thick bamboo, blocks dish and grab handle, the feed gate with the top surface card and the connection of blending tank, the lateral wall of an outer feeding section of thick bamboo is fixed in the lateral wall of a flexible section of thick bamboo, an interior feeding section of thick bamboo sliding connection in the inside of an outer feeding section of thick bamboo, just the bottom of an interior feeding section of thick bamboo run through in the clamp plate, block the lateral wall of dish with the bottom inner wall threaded connection of an interior feeding section of thick bamboo, the bottom mounting of grab handle is in block the top surface of dish.

Preferably, the charging structure further comprises a drain hole, a sealing ring and two slide ways, wherein the drain hole is formed in the side wall of the outer cylinder, the sealing ring is fixed on the inner wall of the inner cylinder, the two slide ways are symmetrically arranged on the inner wall of the inner cylinder, and the sliding block is connected with the slide ways in a sliding manner.

Preferably, the top surface of blowing door is contradicted the bottom surface of sealing ring, just the slide with the vertical distance between the sealing ring is greater than the length of puddler.

Preferably, the elasticity of the spring is greater than the gravity of the discharging structure and the raw material, and the elasticity of the torsion spring is greater than the gravity of the raw material.

Preferably, the stirring structure comprises a first stirring blade and a second stirring blade, the first stirring blade is fixed on the outer wall of the telescopic cylinder, and the second stirring blade is fixed on the outer wall of the rotary cylinder.

Compared with the related technology, the anti-scuticociliatida pharmaceutical composition and the preparation method thereof provided by the invention have the following beneficial effects:

(1) the invention provides a medicinal composition for resisting scuticociliatus pellegrinus and a preparation method thereof, sundew has the effects of resisting bacteria, diminishing inflammation and resisting diseases, and multiple tests prove that the medicinal composition comprises the following components: the Drosera parva has the effect of killing the scuticocida, can obviously reduce the death rate of scuticociliatosis of turbot and reduce the infection rate of scuticocida; the fatty alcohol-polyoxyethylene ether sodium sulfate is colorless, white or light yellow viscous liquid, is an alkaline anionic surfactant with a typical soap smell, is easy to dissolve in water, has excellent decontamination, emulsification, foaming performance and hard water resistance, has LD50 equivalent to edible salt and baking soda, belongs to a nontoxic substance, is a surfactant, can be widely used in the preparation of anti-ciliate medicaments with poor water solubility, plays a role of an auxiliary material of a preparation, and is found by multiple tests that the fatty alcohol-polyoxyethylene ether sodium sulfate has a good effect of killing the shield ciliates; and the mixed solution of the sundew extract extracted by the ethanol and the fatty alcohol-polyoxyethylene ether sodium sulfate has more obvious killing effect on the scuticociliatus sinensis.

(2) The invention provides a medicinal composition for resisting scuticociliatus peltatus and a preparation method thereof, wherein a reaction kettle realizes the integrated production of an extracting solution of Drosera peltatus and a mixed feed, the extracted Drosera peltatus residue is effectively utilized, the traditional two-time ethanol extraction is avoided, in the traditional method, the two-time extraction is generally needed for the ethanol extraction, the crude drug content obtained in the second-time extraction is far lower than that obtained in the first-time extraction, the time consumption is long, and the cost is high. Specifically be through the pneumatic cylinder promotes the clamp plate descends, extrudes the extract in the sundew, drives again the inner tube descends, and the sundew sediment after will drawing is sent to the lower part of blending tank utilizes the inner tube descends, its bottom connect the tooth with the engaging tooth meshing on rotatory section of thick bamboo top is driving through the motor the inner tube rotates, thereby drives rotatory section of thick bamboo rotates, makes the stirring structure can stir mixed liquid and turbot fodder simultaneously, and not only reasonable make full use of raw and other materials has improved production efficiency moreover, practices thrift cost and time, realizes the integration production.

Drawings

FIG. 1 is a schematic view of the overall structure of a reaction vessel according to the present invention;

FIG. 2 is a schematic cross-sectional view of the reaction vessel shown in FIG. 1;

FIG. 3 is a schematic structural view of the charging structure shown in FIG. 2;

FIG. 4 is a schematic top view of the linkage structure shown in FIG. 2;

FIG. 5 is an enlarged view of area A of FIG. 2;

FIG. 6 is a schematic structural view of the discharge structure shown in FIG. 2;

FIG. 7 is a schematic cross-sectional view of the discharge structure shown in FIG. 2;

fig. 8 is an enlarged schematic view of the region B shown in fig. 7.

Reference numbers in the figures: 100. a reaction kettle, 1, a mixing tank, 1a, a discharge port, 2, a supporting structure, 21, a supporting frame, 22, a discharge door, 3, a power structure, 31, a charging port, 32, a hydraulic cylinder, 33, a motor, 34, a telescopic cylinder, 35, a telescopic rod, 36, a pressing plate, 37, a stirring rod, 38, a sliding block, 39, a conductive disc, 39a, a sliding contact line, 4, a feeding structure, 41, a feeding door, 42, an outer feeding cylinder, 43, an inner feeding cylinder, 44, a blocking disc, 45, a grab handle, 5, a charging structure, 51, a partition plate, 52, an outer cylinder, 53, a drain hole, 54, an inner cylinder, 55, a sealing ring, 56, a filter screen, 57, a slide way, 6, a discharging structure, 61, a supporting plate, 62, a discharging door, 63, a first wheel disc, 64, a gear, 65, a second linkage wheel disc, 66, a transmission belt, 67, a sliding cylinder, 68, a spring, 69, a torsion spring, 7, a, 72. the stirring device comprises a rotary cylinder 73, a rotating wheel 74, a support rod 75, a connecting tooth 76, a rack 8, a stirring structure 81, a first stirring blade 82 and a second stirring blade.

Detailed Description

The invention is further described with reference to the following figures and embodiments.

Referring to fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7 and fig. 8, an anti-scuticociliatida pharmaceutical composition is prepared from the following raw materials in parts by weight: 2-3 parts of sundew, 20-30 parts of 95% food-grade ethanol and 0.3-0.6 part of fatty alcohol-polyoxyethylene ether sodium sulfate. A method for producing an anti-scuticociliatida pharmaceutical composition, comprising the steps of:

wherein,

the reaction tank 100 described above includes:

a mixing tank 1; the supporting structure 2 is installed at the bottom end of the mixing tank 1; the power structure 3 is mounted on the top surface of the mixing tank 1, the power structure 3 comprises a hydraulic cylinder 32, a motor 33, a telescopic cylinder 34, a telescopic rod 35 and a pressing plate 36, the hydraulic cylinder 32 is mounted on the top surface of the mixing tank 1, the motor 33 is mounted on the top surface of the hydraulic cylinder 32, the motor 33 is rotatably connected with the hydraulic cylinder 32, the telescopic cylinder 34 is mounted on the bottom surface of the hydraulic cylinder 32, the telescopic rod 35 is slidably connected inside the telescopic cylinder 34, and the top surface of the pressing plate 36 is fixed at the bottom end of the telescopic rod 35; the feeding structure 4 is arranged on the top surface of the mixing tank 1; the charging structure 5 is fixed on the inner wall of the mixing tank 1, the charging structure 5 comprises a partition plate 51, an outer cylinder 52, an inner cylinder 54 and a filter screen 56, the side wall of the partition plate 51 is fixed on the inner wall of the mixing tank 1, the outer cylinder 52 penetrates through the partition plate 51, the outer wall of the inner cylinder 54 is in sliding connection with the inner wall of the outer cylinder 52, and the filter screen 56 is arranged on the upper half part of the inner cylinder 54; the discharging structure 6, the discharging structure 6 is connected with the inner rotation of the inner cylinder 54, the discharging structure 6 includes a supporting plate 61, a discharging door 62, a first wheel disc 63, a gear 64, a second wheel disc 65, a transmission belt 66, a sliding drum 67, a spring 68 and a torsion spring 69, the supporting plate 61 is connected with the inner rotation of the inner cylinder 54, two discharging doors 62 are connected with both sides of the supporting plate 61 in a rotating manner, the first wheel disc 63 is fixed on the side walls of the two discharging doors 62 respectively, the supporting plate 61 is connected with the discharging door 62 through the first wheel disc 63 in a rotating manner, the gear 64 is connected with the bottom end of the supporting plate 61 in a rotating manner, the second wheel disc 65 is fixed on the side surface of the gear 64, the transmission belt 66 is connected with the first wheel disc 63 and the second wheel disc 65, the sliding drum 67 is fixed on the bottom surface of the supporting plate 61, the spring 68 is installed inside the sliding barrel 67, the torsion spring 69 is sleeved on the surface of the first wheel disc 63, one end of the torsion spring 69 is fixed inside the supporting plate 61, and the other end is fixed inside the discharge door 62; the linkage structure 7 is fixed on the inner bottom surface of the mixing tank 1, the linkage structure 7 comprises a cylinder 71, a rotary cylinder 72, a rotating wheel 73, a support rod 74, connecting teeth 75 and a rack 76, the bottom end of the cylinder 71 is fixed on the inner bottom surface of the mixing tank 1, the sliding cylinder 67 is slidably connected inside the cylinder 71, the bottom end of the spring 68 abuts against the top surface of the cylinder 71, the rotary cylinder 72 is rotatably connected on the outer wall of the cylinder 71, the rotating wheel 73 is mounted on the bottom end of the rotary cylinder 72, the rotating wheel 73 is rotatably connected on the inner bottom surface of the mixing tank 1 in a rolling manner, one end of three support rods 74 is fixed on the top end of the rotary cylinder 72 at an equal angle, two connecting teeth 75 are respectively fixed on the other end of three support rods 74 and the bottom end of the inner cylinder 54, and the two connecting teeth 75 are meshed with each other, the two racks 76 are fixed at the top end of the column 71; and the stirring structure 8, wherein the stirring structure 8 is respectively fixed on the outer wall of the telescopic cylinder 34 and the outer wall of the rotary cylinder 72.

Specifically, bearing structure 2 includes support frame 21 and discharge door 22, support frame 21 install in the bottom of blending tank 1, discharge door 22 with the lateral wall of blending tank 1 rotates to be connected, support frame 21 is for inciting somebody to action blending tank 1 supports, discharge door 22 is for conveniently putting into the fodder blending tank 1 lower part, the convenient follow simultaneously the mixed fodder of taking out in the blending tank 1.

Specifically, the power structure 3 further includes a feeding port 31, a plurality of stirring rods 37, sliders 38, conductive discs 39 and a sliding contact line 39a, the feeding port 31 is disposed on the top surface of the mixing tank 1, the plurality of stirring rods 37 are fixed on the bottom surface of the pressing plate 36, the two sliders 38 are symmetrically fixed on the side wall of the pressing plate 36, the conductive discs 39 are mounted on the top surface of the hydraulic cylinder 32, the sliding contact line 39 is mounted inside the motor 33, the sliding contact line 39 is electrically connected with the conductive discs 39, and the feeding port 31 is used for conveniently adding ethanol and sodium alcohol ether sulphate; the stirring rod 37 is used for scattering the sundew which is pressed into blocks in the inner barrel 54 when being put into the lower part of the mixing tank 1, and is conveniently put into the lower part of the mixing tank 1; the sliding block 38 is used for facilitating the up-and-down sliding of the pressing plate 36 in the inner cylinder 54, and simultaneously, the sliding block 38 is matched with the slide way 57 to drive the inner cylinder 54 to rotate, and the conductive disc 39 and the sliding contact line 39a are used for supplying power to the hydraulic cylinder 32 by the motor 33 in the rotating process of the hydraulic cylinder 32.

Specifically, feeding structure 4 includes feed gate 41, an outer feed cylinder 42, an interior feed cylinder 43, blocks dish 44 and grab handle 45, feed gate 41 with the top surface card and the connection of blending tank 1, the lateral wall of an outer feed cylinder 42 is fixed in the lateral wall of a flexible section of thick bamboo 34, interior feed cylinder 43 sliding connection in the inside of an outer feed cylinder 42, just the bottom of an interior feed cylinder 43 run through in clamp plate 36, block the lateral wall of dish 44 with the bottom inner wall threaded connection of an interior feed cylinder 43, the bottom mounting of grab handle 45 block the top surface of dish 44, set up feeding structure 4 puts into in for the convenience of will smashing sundew dish in inner tube 54.

Specifically, the charging structure 5 further includes a drain hole 53, a sealing ring 55 and two slide ways 57, the drain hole 53 is disposed on the side wall of the outer cylinder 52, the sealing ring 55 is fixed on the inner wall of the inner cylinder 54, the two slide ways 57 are symmetrically disposed on the inner wall of the inner cylinder 54, the slider 38 is slidably connected with the slide ways 57, the drain hole 53 is disposed so that when the pressing plate 36 presses the sundew, excess water can permeate the filter screen 56 and be discharged from the drain hole 53, and the sealing ring 55 is disposed so as to ensure the sealing property between the edge of the charging door 62 and the inner wall of the inner cylinder 54, thereby preventing water from leaking to the lower portion of the mixing tank 1.

Specifically, the top surface of the discharge door 62 abuts against the bottom surface of the sealing ring 55, so as to ensure the sealing property between the edge of the discharge door 62 and the inner wall of the inner barrel 54, and prevent water from leaking to the lower part of the mixing tank 1, which causes the feed to be affected with damp; and the vertical distance between the slide way 57 and the sealing ring 55 is greater than the length of the stirring rod 37, so that when the pressing plate 36 slides to the bottom end of the slide way 57, the bottom end of the stirring rod 37 does not abut against the top surface of the discharge door 62.

Specifically, the elastic force of the spring 68 is greater than the gravity of the emptying structure 6 and the raw materials, so that after the sundew and the ethanol are put into the inner barrel 54, the elastic force of the spring 68 can support the support plate 61 and the inner barrel 54 to prevent the support plate 61 and the inner barrel 54 from falling; and the elastic force of the torsion spring 69 is greater than the gravity of the raw material, so that the weight of the sundew and ethanol does not cause the torsion spring 69 to compress after the sundew and ethanol are put into the inner tub 54, thereby causing the discharge door 62 to be opened, so that the ethanol is leaked to the lower portion of the mixing tank 1.

Specifically, the stirring structure 8 includes a first stirring blade 81 and a second stirring blade 82, the first stirring blade 81 is fixed to the outer wall of the telescopic cylinder 34, and the second stirring blade 82 is fixed to the outer wall of the rotary cylinder 72, so as to stir and mix the raw materials in the mixing tank 1.

The preparation process of the medicinal composition for producing the anti-scuticociliatida provided by the invention is as follows:

the reaction kettle is connected with an external power supply. Lifting the feeding door 41 upwards, holding the holding handle 45 and rotating, the holding handle 45 driving the blocking disc 44 to rotate, when the blocking disc 44 loosens, lifting the holding handle 45, pulling the blocking disc 44 out of the outer feeding cylinder 42 and the inner feeding cylinder 43, then taking 2-3 parts of crushed sundew according to the weight parts, adding the crushed sundew into the inner cylinder 54 through the outer feeding cylinder 42 and the inner feeding cylinder 43, then putting the holding handle 45 and the blocking disc 44 into the outer feeding cylinder 42 and the inner feeding cylinder 43, and rotating the holding handle 45, the holding handle 45 driving the blocking disc 44 to rotate, connecting with the inner thread at the bottom end of the inner feeding cylinder 43, sealing the bottom end of the inner feeding cylinder 43, taking 20-30 parts of ethanol with the food grade concentration of 95% to be added into the upper part of the mixing tank 1 through the feeding port 31, ethanol enters the inner cylinder 54 through the filter screen 56 to soak sundew, then the discharge door 22 is opened, and 1000 parts of 800-flavored turbot feed are put into the lower half part of the mixing tank 1 through the discharge door 22; the motor 33 is started, the motor 33 drives the hydraulic cylinder 32 to rotate, the conductive disc 39 rotates along with the hydraulic cylinder 32, the sliding contact line 39a is electrically connected with the conductive disc 39 all the time, the hydraulic cylinder 32 is powered on all the time, meanwhile, the hydraulic cylinder 32 drives the telescopic cylinder 34, the telescopic rod 35, the outer feeding cylinder 42, the inner feeding cylinder 43, the pressing plate 36 and the stirring rod 37 to rotate together, the telescopic cylinder 34 drives the first stirring blade 81 to rotate to stir an extracting solution, the sliding block 38 on the pressing plate 36 props against the sliding channel 57, so that the inner cylinder 54 rotates along with the pressing plate 36, and the stirring rod 37 stirs sundew to fully contact the sundew with ethanol; the lower part of the inner cylinder 54 is made of solid material, so that the drain hole 53 on the side wall of the outer cylinder 52 is blocked, the support plate 61 and the discharge door 62 in the mixing tank 1 are stationary, after ethanol in the upper half part of the mixing tank 1 is extracted for 5 hours, the motor 33 is stopped to rotate, the hydraulic cylinder 32 is started, the telescopic rod 35 extends out of the telescopic cylinder 34, so that the press plate 36 drives the slide block 38 to slide downwards in the slide way 57, the inner feed cylinder 43 extends simultaneously, the press plate 36 presses the sundew, extract in the sundew is pressed out and discharged through the filter screen 56, when the press plate 36 reaches the bottom end of the slide way 57, the press plate 36 starts to drive the inner cylinder 54 to move downwards, the inner cylinder 54 drives the support plate 61 and the discharge door 62 to move downwards, and the torsion spring 69 has enough elasticity to support the discharge door 62, therefore, the discharge door 62 is not opened, the drain hole 53 communicates with the filter screen 56 as the inner cylinder 54 moves downward, and the residual extract in the sundew can be discharged through the drain hole 53; the supporting plate 61 can drive in-process that descends slide cartridge 67 is in the inside slip of cylinder 71, simultaneously spring 68 is compressed, gear 64 can with the rack 76 meshing on cylinder 71 top, rack 76 drives gear 64 rotates, gear 64 drives second rim plate 65 rotates, second rim plate 65 passes through drive belt 66 drives first rim plate 63 rotates, first rim plate 63 drives discharge door 62 rotates, discharge door 62 opens, sundew in the inner tube 54 gets into the lower part of blending tank 1, at this moment with 0.3-0.6 part fatty alcohol polyoxyethylene ether sodium sulfate add to the extract. When the discharge door 62 is opened, the torsion spring 69 is compressed, when the expansion link 35 extends to the longest, the connecting teeth 75 at the bottom end of the inner cylinder 54 are just meshed with the connecting teeth 75 at the top end of the rotary cylinder 72, the tooth shape of the connecting teeth 75 is arc-shaped, even if the connecting teeth 75 at the bottom end of the inner cylinder 54 are not meshed with the connecting teeth 75 at the top end of the rotary cylinder 72 when in collision, because the connecting teeth 75 are arc-shaped at one side, the falling force of the inner cylinder 54 can make the connecting teeth 75 at the top end of the rotary cylinder 72 rotate a little, so that the two connecting teeth 75 can be meshed together smoothly, the motor 33 is turned on, the rotating cylinder 72 is driven to rotate by the meshing of the connecting teeth 75, the rotating wheel 73 rolls on the inner bottom surface of the mixing tank 1, and the second stirring blade 82 is driven to rotate by the rotary cylinder 72, the stirring rod 37 stirs the sundew slag in the filter screen 56, so that the extracted sundew slag can easily fall into the lower part of the mixing tank 1, and the turbot feed and the extracted sundew slag can be fully mixed together; meanwhile, the first stirring blade 81 rotates to fully mix the extracting solution with the fatty alcohol-polyoxyethylene ether sodium sulfate. After the second stirring blade 82 is fully stirred for 0.5h, the motor 33 is turned off, the hydraulic cylinder 32, the telescopic cylinder 34, the telescopic rod 35, the pressing plate 36, the inner cylinder 54 and the rotary cylinder 72 stop rotating, then the mixed liquid discharge port 1a at the upper part of the mixing tank 1 is taken out, and the mixed feed at the lower part of the mixing tank 1 is taken out through the discharge door 22; then the hydraulic cylinder 32 is controlled to lift the telescopic rod 35, the telescopic rod 35 drives the pressing plate 36 and the stirring rod 37 and the inner feeding cylinder 43 to ascend, the telescopic rod 35 slides in the telescopic cylinder 34, the inner feeding cylinder 43 slides in the outer feeding cylinder 42, the pressing plate 36 drives the sliding block 38 to slide in the slideway 57, the inner cylinder 54 loses the pressure of the pressing plate 36, the spring 68 is reset, the sliding cylinder 67 slides out of the cylinder 71, the spring 38 jacks up the supporting plate 61 and the discharging door 62, the supporting plate 61 jacks up the inner cylinder 54, the inner cylinder 54 slides in the outer cylinder 52, the connecting teeth 75 at the bottom end of the inner cylinder 54 are separated from the connecting teeth 75 at the top end of the rotating cylinder 72, the rack 76 drives the gear 64 to rotate reversely, and the gear 64 drives the second wheel disc 65 to rotate reversely, the second wheel disc 65 drives the first wheel disc 63 to rotate through the transmission belt 66, the first wheel disc 63 drives the discharge door 62 to rotate reversely, meanwhile, the torsion spring 69 is reset, finally, the discharge door 62 is closed, the top surface of the discharge door abuts against the bottom surface of the sealing ring 55, when the telescopic rod 35 ascends to the initial position, the hydraulic cylinder 72 stops working, meanwhile, the spring 68 also resets, and the inner cylinder 54 returns to the initial position.

Example 1: preparing sundew powder: 230g of sundew medicinal material is taken, crushed and sieved by a 200-mesh sieve.

Usage amounts of example 1: the powder is mixed with 200 kg of feed and fed according to daily food consumption.

Example 2: preparation of sodium fatty Alcohol Ether Sulfate (AES) liquid formulation: and adding 4g of fatty alcohol-polyoxyethylene ether sodium sulfate (AES) into 200mL of food-grade 95% ethanol, and uniformly mixing to obtain the composition.

Usage and dosage of example 2: taking 1L of the extract of example 2, sprinkling to 2000kg of seawater, and carrying out medicated bath of Scophthalmus maximus suffering from scuticociliatosis for 30 minutes per day, and continuously carrying out medicated bath for 5-7 days.

Example 3: preparing a sundew liquid preparation: taking 200g of Drosera parviflora, crushing, adding 10 times of food-grade 95% ethanol, and soaking and extracting for 5h by shaking a table.

Usage amounts of example 3: taking 1L of the extract of example 3, sprinkling to 2000kg of seawater, and carrying out medicated bath of Scophthalmus maximus suffering from scuticociliatosis for 30 minutes per day, and continuously carrying out medicated bath for 5-7 days.

Example 4: preparation of sundew and sodium Alcohol Ether Sulfate (AES) preparation: 200g of sundew is taken, crushed, extracted by 10 times of food-grade 95% ethanol, and added with 40g of sodium Alcohol Ether Sulphate (AES).

Usage amounts of example 4: taking 1L of the extract of example 4, sprinkling to 2000kg of seawater, and carrying out medicated bath of Scophthalmus maximus suffering from scuticociliatosis for 30 minutes per day, and continuously carrying out medicated bath for 5-7 days.

The killing effect of examples 2, 3, 4 on ciliates was observed.

The effect of examples 2, 3, 4 on the killing of scuticociliatus ciliates is illustrated below in connection with in vitro insecticidal tests.

In vitro insecticidal test: taking a 96-deep-hole plate, firstly adding 0.6mL of seawater into each hole, then adding 0.6mL of the embodiment 2, diluting by times, and diluting to the 12 th hole, wherein the concentration of the medicines is 1/2-1/4096 respectively; according to the method, the samples of example 3, 4 and 95% ethanol are diluted in sequence, 120 mu L of diluted sample is absorbed and added into a 96-hole cell culture plate, then 120 mu L of ciliate solution is added into each hole, the cell culture plate is slightly shaken for 1 minute and then kept still for 2 hours, and an inverted microscope is used for observing whether the ciliates die or not to judge the drug effect. Meanwhile, a blank control group is set, and the concentration of the ciliate peltate is the same as that of the test group. The results show that: the 95% ethanol solvent control group had an insecticidal concentration of 1:8, the in vitro insecticidal concentration of example 2 was 1:2048, the in vitro insecticidal concentration of example 3 was 1:1024, and the in vitro insecticidal concentration of example 4 was 1: 8192. Through in vitro insecticidal test, the combined use of the sundew and the fatty alcohol-polyoxyethylene ether sodium sulfate (AES) can obviously improve the insecticidal effect, the same insecticidal effect is achieved, the use concentration of the sundew and the fatty alcohol-polyoxyethylene ether sodium sulfate (AES) is 25% of that of the sundew alone, and is 12.5% of that of the fatty alcohol-polyoxyethylene ether sodium sulfate (AES) alone, and the use amounts of the two materials can be obviously reduced.

The drug effects of the examples 1, 2, 3 and 4 on preventing and treating sculellaria maximus paralichthys olivaceus ciliophora are observed.

The Drosera peltata resistance test is described below in conjunction with specific tests.

Constructing a turbot virus attacking model: the larva of scuticociliatus gibbosa in exponential growth phase is used for counteracting toxic substance. Rubbing 2cm × 2cm abrasion area with iron gauze on back muscle of turbot (weight about 200 g), adding 50L water for fish culture at 50000/L, and oxygenating at 12 deg.C to normally feed turbot. The change of the ulcer part is observed every day, mucus or tissue is scraped off from the wound part lightly, the picture is observed and photographed under a microscope and counted, and the turbot scurvy ciliate disease is established 2 days after infection.

And (3) pharmacodynamic test: dividing into 6 groups, each group has 20 tails, and each group is provided with parallel groups, wherein the first group is a model control group: disease model groups without any drug treatment; the second group is the test group of example 1, which is challenged and added with example 1 in the feed and fed every day; the third, fourth and fifth groups were tested by medicated bath method in examples 2, 3 and 4, respectively, and the dosage of the medicated bath used in examples 2, 3 and 4 was 1L of the extract sprayed into 2000kg of seawater, and the Scophthalmus maximus suffering from scurf disease was medicated bath for 30 minutes each day, and the sixth group was a healthy control group. Test results the affected parts of the test group and the control group were gently scraped by an inoculating loop, and the activity and the number of ciliates were observed under a microscope. The test was carried out for 7 days, infection rate = (number of infections + number of deaths)/20 × 100%, mortality rate = number of deaths/20 × 100%. The results are shown in Table 1.

TABLE 1 Effect of the examples against ciliate peltate disease

Test results show that the sundew and the fatty alcohol-polyoxyethylene ether sodium sulfate (AES) can kill aquatic scuticociliatida ciliates, and the effect is obviously enhanced after the sundew and the fatty alcohol-polyoxyethylene ether sodium sulfate are combined. The sundew and the fatty alcohol-polyoxyethylene ether sodium sulfate (AES) have obvious curative effects on preventing and treating turbot scuticociliatosis, can reduce the death rate and infection rate of the turbot scuticociliatosis, are safer compared with chemical medicines such as formaldehyde, copper sulfate and the like, ensure the food safety, do not cause obvious influence on the feeding of fish, do not influence the growth of the turbot, and reduce the economic loss of farmers. Meanwhile, the use of chemical drugs such as formaldehyde, copper sulfate and the like is reduced, and the method has far-reaching significance for marine environmental protection.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. An anti-scuticociliatida pharmaceutical composition is characterized by being prepared from the following raw materials in parts by weight: 2-3 parts of sundew, 20-30 parts of 95% food-grade ethanol and 0.3-0.6 part of fatty alcohol-polyoxyethylene ether sodium sulfate.

2. A production method of an anti-scuticociliatida pharmaceutical composition is characterized by comprising the following steps:

wherein,

the reaction vessel (100) described above comprises:

a mixing tank (1);

a support structure (2), the support structure (2) being mounted at the bottom end of the mixing tank (1);

the power structure (3) is installed on the top surface of the mixing tank (1), the power structure (3) comprises a hydraulic cylinder (32), a motor (33), a telescopic cylinder (34), a telescopic rod (35) and a pressing plate (36), the hydraulic cylinder (32) is installed on the top surface of the mixing tank (1), the motor (33) is installed on the top surface of the hydraulic cylinder (32), the motor (33) is rotatably connected with the hydraulic cylinder (32), the telescopic cylinder (34) is installed on the bottom surface of the hydraulic cylinder (32), the telescopic rod (35) is slidably connected inside the telescopic cylinder (34), and the top surface of the pressing plate (36) is fixed to the bottom end of the telescopic rod (35);

the feeding structure (4), the feeding structure (4) is arranged on the top surface of the mixing tank (1);

the charging structure (5) is fixed on the inner wall of the mixing tank (1), the charging structure (5) comprises a partition plate (51), an outer cylinder (52), an inner cylinder (54) and a filter screen (56), the side wall of the partition plate (51) is fixed on the inner wall of the mixing tank (1), the outer cylinder (52) penetrates through the partition plate (51), the outer wall of the inner cylinder (54) is in sliding connection with the inner wall of the outer cylinder (52), and the filter screen (56) is arranged on the upper half part of the inner cylinder (54);

the material placing structure (6) is connected with the inner portion of the inner barrel (54) in a rotating mode, the material placing structure (6) comprises a supporting plate (61), a material placing door (62), a first wheel disc (63), a gear (64), a second wheel disc (65), a transmission belt (66), a sliding barrel (67), a spring (68) and a torsion spring (69), the supporting plate (61) is connected with the inner portion of the inner barrel (54) in a rotating mode, the two material placing doors (62) are connected with two sides of the supporting plate (61) in a rotating mode, the first wheel disc (63) is fixed on two side walls of the material placing door (62) respectively, the supporting plate (61) is connected with the material placing door (62) in a rotating mode through the first wheel disc (63), the gear (64) is connected with the bottom end of the supporting plate (61) in a rotating mode, and the second wheel disc (65) is fixed on the side face of the gear (64), the transmission belt (66) is connected with the first wheel disc (63) and the second wheel disc (65), the sliding barrel (67) is fixed on the bottom surface of the supporting plate (61), the spring (68) is installed inside the sliding barrel (67), the torsion spring (69) is sleeved on the surface of the first wheel disc (63), one end of the torsion spring (69) is fixed inside the supporting plate (61), and the other end of the torsion spring is fixed inside the discharge door (62);

the linkage structure (7) is fixed on the inner bottom surface of the mixing tank (1), the linkage structure (7) comprises a cylinder (71), a rotating cylinder (72), a rotating wheel (73), a support rod (74), connecting teeth (75) and a rack (76), the bottom end of the cylinder (71) is fixed on the inner bottom surface of the mixing tank (1), the sliding cylinder (67) is connected in the cylinder (71) in a sliding manner, the bottom end of the spring (68) is abutted against the top surface of the cylinder (71), the rotating cylinder (72) is rotatably connected on the outer wall of the cylinder (71), the rotating wheel (73) is installed at the bottom end of the rotating cylinder (72), the rotating wheel (73) is rotatably connected on the inner bottom surface of the mixing tank (1) in a rolling manner, one end of the three support rods (74) is fixed on the top end of the rotating cylinder (72) at an equal angle, the two connecting teeth (75) are respectively fixed at the other ends of the three support rods (74) and the bottom end of the inner cylinder (54), the two connecting teeth (75) are meshed with each other, and the two racks (76) are fixed at the top end of the column body (71);

the stirring structure (8), the stirring structure (8) is fixed on the outer wall of the telescopic cylinder (34) and the outer wall of the rotary cylinder (72) respectively.

3. The method for producing a ciliate-resistant pharmaceutical composition according to claim 2, wherein said supporting structure (2) includes a support frame (21) and an exit door (22), said support frame (21) being mounted at the bottom end of said mixing tank (1), said exit door (22) being rotatably connected to the side wall of said mixing tank (1).

4. The method for producing the scuticociliatida-resistant pharmaceutical composition according to claim 2, wherein said power structure (3) further comprises a feeding port (31), a plurality of stirring rods (37), a sliding block (38), a conductive disc (39) and a sliding wire (39 a), said feeding port (31) is disposed on the top surface of said mixing tank (1), said plurality of stirring rods (37) are fixed on the bottom surface of said pressing plate (36), two of said sliding blocks (38) are symmetrically fixed on the side wall of said pressing plate (36), said conductive disc (39) is mounted on the top surface of said hydraulic cylinder (32), said sliding wire (39) is mounted inside said motor (33), and said sliding wire (39) and said conductive disc (39) are electrically connected.

5. The method for producing the scuticociliatida-resistant pharmaceutical composition according to claim 2, wherein the feeding structure (4) comprises a feeding gate (41), an outer feeding cylinder (42), an inner feeding cylinder (43), a blocking disk (44) and a handle (45), the feeding gate (41) is clamped and connected with the top surface of the mixing tank (1), the side wall of the outer feeding cylinder (42) is fixed on the side wall of the telescopic cylinder (34), the inner feeding cylinder (43) is slidably connected with the inside of the outer feeding cylinder (42), the bottom end of the inner feeding cylinder (43) penetrates through the pressing plate (36), the side wall of the blocking disk (44) is in threaded connection with the bottom end inner wall of the inner feeding cylinder (43), and the bottom end of the handle (45) is fixed on the top surface of the blocking disk (44).

6. The method for producing the ciliate-resistant pharmaceutical composition of claim 4, wherein the loading structure (5) further includes a drainage hole (53), a sealing ring (55) and a slide way (57), the drainage hole (53) is disposed on the side wall of the outer cylinder (52), the sealing ring (55) is fixed on the inner wall of the inner cylinder (54), the two slide ways (57) are symmetrically disposed on the inner wall of the inner cylinder (54), and the slide block (38) is slidably connected with the slide way (57).

7. The method of claim 6, wherein the top surface of the discharge gate (62) abuts the bottom surface of the sealing ring (55), and the vertical distance between the chute (57) and the sealing ring (55) is greater than the length of the stirring rod (37).

8. The method for producing the scuticociliatida-resistant pharmaceutical composition according to claim 4, wherein the elastic force of said spring (68) is greater than the gravity of the emptying structure (6) and the raw material, and the elastic force of said torsion spring (69) is greater than the gravity of the raw material.

9. The method for producing an anti-scuticociliatus sinensis pharmaceutical composition as claimed in claim 4, wherein said stirring structure (8) comprises a first stirring blade (81) and a second stirring blade (82), said first stirring blade (81) being fixed to the outer wall of said telescopic cylinder (34), said second stirring blade (82) being fixed to the outer wall of said rotary cylinder (72).