CN114146133A - 宽叶苔草在制备具有抗衰老功效产品中的应用 - Google Patents
宽叶苔草在制备具有抗衰老功效产品中的应用 Download PDFInfo
- Publication number
- CN114146133A CN114146133A CN202111572219.5A CN202111572219A CN114146133A CN 114146133 A CN114146133 A CN 114146133A CN 202111572219 A CN202111572219 A CN 202111572219A CN 114146133 A CN114146133 A CN 114146133A
- Authority
- CN
- China
- Prior art keywords
- ethyl acetate
- extract
- latifolia
- carex
- extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000003712 anti-aging effect Effects 0.000 title claims abstract description 19
- 241001122192 Carex breviculmis Species 0.000 title abstract description 15
- 238000002360 preparation method Methods 0.000 title abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000002024 ethyl acetate extract Substances 0.000 claims abstract description 25
- 239000000284 extract Substances 0.000 claims abstract description 23
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000002904 solvent Substances 0.000 claims abstract description 15
- 238000000605 extraction Methods 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 239000010410 layer Substances 0.000 claims abstract description 7
- 238000010992 reflux Methods 0.000 claims abstract description 6
- 238000002791 soaking Methods 0.000 claims abstract description 6
- 238000010438 heat treatment Methods 0.000 claims abstract description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 4
- 239000004744 fabric Substances 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 239000012044 organic layer Substances 0.000 claims abstract description 4
- 241000722731 Carex Species 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 5
- 241001290610 Abildgaardia Species 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 239000002537 cosmetic Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 2
- 230000036541 health Effects 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 210000002950 fibroblast Anatomy 0.000 abstract description 8
- 108010016113 Matrix Metalloproteinase 1 Proteins 0.000 abstract description 7
- 102000000380 Matrix Metalloproteinase 1 Human genes 0.000 abstract description 7
- 102000008186 Collagen Human genes 0.000 abstract description 4
- 229920001436 collagen Polymers 0.000 abstract description 4
- 241000195940 Bryophyta Species 0.000 abstract description 3
- 108010035532 Collagen Proteins 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 238000001704 evaporation Methods 0.000 abstract 1
- 238000010025 steaming Methods 0.000 abstract 1
- 241000319930 Bryophyllum <angiosperm> Species 0.000 description 10
- 230000032683 aging Effects 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 7
- 210000001339 epidermal cell Anatomy 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 102000006587 Glutathione peroxidase Human genes 0.000 description 5
- 108700016172 Glutathione peroxidases Proteins 0.000 description 5
- 102000019197 Superoxide Dismutase Human genes 0.000 description 5
- 108010012715 Superoxide dismutase Proteins 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000005936 beta-Galactosidase Human genes 0.000 description 4
- 108010005774 beta-Galactosidase Proteins 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 241001244316 Carex brevicaulis Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical class CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000005786 degenerative changes Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241001166575 Carex platyphylla Species 0.000 description 1
- 102100028717 Cytosolic 5'-nucleotidase 3A Human genes 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000219745 Lupinus Species 0.000 description 1
- 101150014058 MMP1 gene Proteins 0.000 description 1
- 235000003805 Musa ABB Group Nutrition 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 235000015266 Plantago major Nutrition 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 241000220286 Sedum Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000032677 cell aging Effects 0.000 description 1
- 230000023402 cell communication Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 239000008004 cell lysis buffer Substances 0.000 description 1
- 230000005754 cellular signaling Effects 0.000 description 1
- 239000002026 chloroform extract Substances 0.000 description 1
- 108700004333 collagenase 1 Proteins 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000002035 hexane extract Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000004065 mitochondrial dysfunction Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007423 screening assay Methods 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 102000055501 telomere Human genes 0.000 description 1
- 108091035539 telomere Proteins 0.000 description 1
- 210000003411 telomere Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/89—Cyperaceae (Sedge family)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Birds (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
宽叶苔草在制备具有抗衰老功效产品中的应用，涉及宽叶苔草新用途领域，其中，宽叶苔草为宽叶苔草乙酸乙酯提取物，其制备方法为：取宽叶苔草加75％乙醇浸泡；水浴加热回流提取；用绢布滤出提取液，合并回收溶剂；将提取液置于水浴锅上蒸至无醇味；加入乙酸乙酯，振摇萃取，合并有机层，回收溶剂，水层蒸至无试剂味后进行下一次萃取；回收溶剂，获得浸膏；用热水重新溶解浸膏，过滤后即得宽叶苔草乙酸乙酯提取物。本发明通过试验证实宽叶苔草乙酸乙酯提取物显著增强了SOD和GPX的表达，显著抑制了SABG的表达；增强了成纤维细胞HSF胶原蛋白的产生且抑制了基质金属蛋白酶‑1的表达。由此证明宽叶苔草乙酸乙酯提取物具有抗衰老功效。
Description
技术领域
本发明涉及宽叶苔草新用途技术领域，具体涉及一种宽叶苔草在制备具有抗衰老功效产品中的应用。
背景技术
衰老是一种不可抗拒的自然规律，是每个有生命的个体生长发育的必经阶段，常表现为结构的退行性变化和机能的衰退。衰老可以分为两类：一类是生理性衰老，指机体在成熟期后出现的生理退化过程，主要体现在各器官组织功能随年龄增长而发生的退行性变化；二类是病理性衰老，即因患某些疾病或受外界因素的影响，而加速衰老的过程。
2013年已有学者在《Cell》杂志提出衰老的9个重要因素，这些因素又可以根据其与衰老的关系密切程度分为三级，其中基因组不稳定、端粒缩短、表观遗传学改变、蛋白稳定性改变为一级因素，对营养物质的反馈失衡、线粒体功能障碍、细胞衰老为二级因素，干细胞衰竭、细胞通讯发生变化为三级因素。
衰老过程是不可逆的，但一些药物可以延缓及预防衰老。例如公开号为CN106109362A的中国专利公开了一种抗衰老组合物、抗衰老化妆品及其制备方法，它是通过将车前草籽提取物、羽扇豆提取物以及荷花提取物复配使用起到改善皮肤老化、紧致皮肤等作用。
宽叶苔草常被作为园林景观植物，用作乔木下及低湿处的地被。2017年西北农林科技大学的郭恩辉将宽叶苔草提取物作为植物源抑菌物质，证明了宽叶苔草提取物对多种病原微生物有抑制作用。
目前，宽叶苔草提取物在抗衰老方面的应用尚未见报道。
发明内容
本发明的目的是提供一种宽叶苔草在制备具有抗衰老功效产品中的应用。
本发明为解决技术问题所采用的技术方案如下：
本发明的宽叶苔草在制备具有抗衰老功效产品中的应用。
作为优选的实施方式，所述宽叶苔草为宽叶苔草乙酸乙酯提取物。
作为更优选的实施方式，所述宽叶苔草乙酸乙酯提取物按照以下方法制备：
①称取宽叶苔草，加4-6倍量75％乙醇浸泡10-14h；
②水浴加热回流提取2次，每次1.5-2.5h；用绢布滤出提取液，合并回收溶剂；
③将回收完溶剂的提取液置于水浴锅上蒸至无醇味；
④按提取液与乙酸乙酯的体积比为1:1的比例向上述提取液中加入乙酸乙酯，振摇萃取4-6次，合并有机层，回收溶剂，其中，水层置于水浴锅蒸至无试剂味后再进行下一次萃取；
⑤经萃取获得正水层部分，回收溶剂，获得浸膏；
⑥用40-70℃热水重新溶解浸膏，经0.22μm滤膜过滤后即得宽叶苔草乙酸乙酯提取物。
作为更优选的实施方式，步骤①中，加5倍量75％乙醇浸泡12h。
作为更优选的实施方式，步骤②中，水浴加热回流提取2次，每次2h。
作为更优选的实施方式，步骤④中，振摇萃取5次。
作为更优选的实施方式，步骤⑥中，用温度为50℃的热水重新溶解浸膏。
作为优选的实施方式，所述宽叶苔草乙酸乙酯提取物的作用浓度范围为：0.01-1mg/ml。
作为更优选的实施方式，所述宽叶苔草乙酸乙酯提取物的浓度为：0.825mg/ml。
作为优选的实施方式，所述产品包括：食品、药品、保健品和化妆品。
本发明的有益效果是：
本发明通过试验证实宽叶苔草乙酸乙酯提取物显著增强了抗衰老超氧化物歧化酶SOD和谷胱甘肽过氧化物酶GPX的表达，同时显著抑制了β-半乳糖苷酶SABG的表达；并增强了成纤维细胞HSF胶原蛋白的产生且抑制了成纤维细胞HSF基质金属蛋白酶-1(MMP-1)的表达。由此证实，宽叶苔草乙酸乙酯提取物具有调节抗氧化相关基因表达的作用，从而达到抗衰老的功效。
本发明所提供的宽叶苔草乙酸乙酯提取物在制备具有抗衰老功效产品中的应用开拓了宽叶苔草的新用途，为开发新型的抗衰老抗氧化功效产品提供了新的思路。
附图说明
图1为实施例3中宽叶苔草乙酸乙酯提取物抗衰老作用试验结果。
具体实施方式
下面将结合本发明实施例，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
试验材料
宽叶苔草，来源于吉林大学天然药物研究中心。
人表皮细胞Hacat，购买自上海酶研生物科技有限公司。
成纤维细胞HSF，购买自上海酶研生物科技有限公司。
细胞裂解液Cell Lysis Buffer，购买自Cell Signaling Technology。
实施例1宽叶苔草提取物的制备
①称取宽叶苔草250g，加5倍量75％乙醇浸泡12h。
②水浴加热回流提取2次，每次2h；用绢布滤出提取液，合并回收溶剂。
③将回收完溶剂的提取液置于水浴锅上蒸至无醇味，提取液终体积控制在500ml左右。
④向上述提取液中加入乙酸乙酯500ml，振摇萃取5次，合并有机层，回收溶剂，其中，水层置于水浴锅蒸至无试剂味后再进行下一次萃取。
同时，以正己烷、氯仿、水饱和正丁醇这三种有机溶剂做平行试验，试验过程同上。
⑤经过上述萃取获得正水层部分，回收溶剂，获得浸膏。
⑥用50℃热水重新溶解浸膏，过0.22μm滤膜，即得宽叶苔草提取物，即宽叶苔草乙酸乙酯提取物、宽叶苔草正己烷提取物、宽叶苔草氯仿提取物、宽叶苔草水饱和正丁醇提取物。
实施例2功效筛选试验
(1)细胞给药
①用含有10％胎牛血清的DMEM(高糖)培养人表皮细胞Hacat，待细胞生长至80-90％密度时消化并传至96孔板。
②24h后待细胞生长至70-80％密度时，将实施例1中经过滤后的各宽叶苔草提取物分别用含10％胎牛血清的DMEM培养液稀释至1.65mg/ml、0.825mg/ml、0.413mg/ml、0.206mg/ml、0.103mg/ml、0.052mg/ml共6个不同浓度并加入人表皮细胞Hacat继续培养。
③继续培养24h后，加入细胞裂解液Cell Lysis Buffer，充分裂解细胞。
(2)功效筛选
24h后收集细胞提取总RNA，通过基因芯片技术检测转录组变化，通过生物信息分析由药物作用引起的表皮细胞基因变化及涉及的生物学功能，结果发现只有宽叶苔草乙酸乙酯提取物具有上调超氧化物歧化酶SOD、谷胱甘肽过氧化物酶GPX和抑制β-半乳糖苷酶SABG的作用，因此，初步推测宽叶苔草乙酸乙酯提取物具有抗衰老功效。
实施例3效果验证性试验
以人表皮细胞Hacat和成纤维细胞HSF为模型，分别以不同浓度宽叶苔草乙酸乙酯提取物作用于人表皮细胞Hacat后，qPCR检测其衰老相关酶(超氧化物歧化酶SOD、谷胱甘肽过氧化物酶GPX、β-半乳糖苷酶SABG)的表达变化；同时，检测宽叶苔草乙酸乙酯提取物作用于成纤维细胞HSF后，胶原蛋白、基质金属蛋白酶-1(MMP-1)的表达变化。其中，阴性对照采用含10％胎牛血清的DMEM做相同处理。检测结果如表1和图1所示。
表1检测结果
由表1和图1可知，宽叶苔草乙酸乙酯提取物显著增强了抗衰老超氧化物歧化酶SOD和谷胱甘肽过氧化物酶GPX的表达，同时显著抑制了β-半乳糖苷酶SABG的表达；并增强了成纤维细胞HSF胶原蛋白的产生且抑制了成纤维细胞HSF基质金属蛋白酶-1(MMP-1)的表达。
综上，宽叶苔草乙酸乙酯提取物具有调节抗氧化相关基因表达的作用，从而达到抗衰老的作用。
以上所述仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。
Claims (10)
1.宽叶苔草在制备具有抗衰老功效产品中的应用。
2.根据权利要求1所述的应用，其特征在于，所述宽叶苔草为宽叶苔草乙酸乙酯提取物。
3.根据权利要求2所述的应用，其特征在于，所述宽叶苔草乙酸乙酯提取物按照以下方法制备：
①称取宽叶苔草，加4-6倍量75％乙醇浸泡10-14h；
②水浴加热回流提取2次，每次1.5-2.5h；用绢布滤出提取液，合并回收溶剂；
③将回收完溶剂的提取液置于水浴锅上蒸至无醇味；
④按提取液与乙酸乙酯的体积比为1:1的比例向上述提取液中加入乙酸乙酯，振摇萃取4-6次，合并有机层，回收溶剂，其中，水层置于水浴锅蒸至无试剂味后再进行下一次萃取；
⑤经萃取获得正水层部分，回收溶剂，获得浸膏；
⑥用40-70℃热水重新溶解浸膏，经0.22μm滤膜过滤后即得宽叶苔草乙酸乙酯提取物。
4.根据权利要求3所述的应用，其特征在于，步骤①中，加5倍量75％乙醇浸泡12h。
5.根据权利要求3所述的应用，其特征在于，步骤②中，水浴加热回流提取2次，每次2h。
6.根据权利要求3所述的应用，其特征在于，步骤④中，振摇萃取5次。
7.根据权利要求3所述的应用，其特征在于，步骤⑥中，用温度为50℃的热水重新溶解浸膏。
8.根据权利要求2所述的应用，其特征在于，所述宽叶苔草乙酸乙酯提取物的作用浓度范围为：0.01-1mg/ml。
9.根据权利要求8所述的应用，其特征在于，所述宽叶苔草乙酸乙酯提取物的浓度为：0.825mg/ml。
10.根据权利要求1所述的应用，其特征在于，所述产品包括：食品、药品、保健品和化妆品。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111572219.5A CN114146133A (zh) | 2021-12-21 | 2021-12-21 | 宽叶苔草在制备具有抗衰老功效产品中的应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111572219.5A CN114146133A (zh) | 2021-12-21 | 2021-12-21 | 宽叶苔草在制备具有抗衰老功效产品中的应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114146133A true CN114146133A (zh) | 2022-03-08 |
Family
ID=80451858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111572219.5A Pending CN114146133A (zh) | 2021-12-21 | 2021-12-21 | 宽叶苔草在制备具有抗衰老功效产品中的应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114146133A (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115812778A (zh) * | 2023-02-20 | 2023-03-21 | 山东凯欣绿色农业发展股份有限公司 | 新型鲜果清洁保鲜一体生产工艺 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859816A (zh) * | 2016-05-03 | 2016-08-17 | 浙江大学 | 火麻仁中提取β-谷甾醇和β-胡萝卜苷的方法、应用及产品 |
| KR20160098571A (ko) * | 2015-02-09 | 2016-08-19 | 순천향대학교 산학협력단 | 천일사초 추출물을 함유하는 항염증 항산화 활성의 약학조성물 및 식품조성물 |
| KR20160105586A (ko) * | 2015-02-27 | 2016-09-07 | 순천향대학교 산학협력단 | 사초과 식물 추출물을 함유하는 화장품 조성물 및 그 제조방법 |
| KR102086854B1 (ko) * | 2018-10-05 | 2020-03-11 | 대한민국 | 사초과 식물 추출물을 함유하는 항산화 또는 항염용 화장료 조성물 |
-
2021
- 2021-12-21 CN CN202111572219.5A patent/CN114146133A/zh active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160098571A (ko) * | 2015-02-09 | 2016-08-19 | 순천향대학교 산학협력단 | 천일사초 추출물을 함유하는 항염증 항산화 활성의 약학조성물 및 식품조성물 |
| KR20160105586A (ko) * | 2015-02-27 | 2016-09-07 | 순천향대학교 산학협력단 | 사초과 식물 추출물을 함유하는 화장품 조성물 및 그 제조방법 |
| CN105859816A (zh) * | 2016-05-03 | 2016-08-17 | 浙江大学 | 火麻仁中提取β-谷甾醇和β-胡萝卜苷的方法、应用及产品 |
| KR102086854B1 (ko) * | 2018-10-05 | 2020-03-11 | 대한민국 | 사초과 식물 추출물을 함유하는 항산화 또는 항염용 화장료 조성물 |
Non-Patent Citations (2)
| Title |
|---|
| 杨波: "宽叶苔草脂溶性化学成分研究" * |
| 杨波等: "宽叶苔草脂溶性化学成分研究", 《时珍国医国药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115812778A (zh) * | 2023-02-20 | 2023-03-21 | 山东凯欣绿色农业发展股份有限公司 | 新型鲜果清洁保鲜一体生产工艺 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lau et al. | Ethnomedicinal uses, pharmacological activities, and cultivation of Lignosus spp.(tiger׳ s milk mushrooms) in Malaysia–A review | |
| CN105647974B (zh) | 一种酿酒酵母植物发酵物及其应用和皮肤外用剂 | |
| CN108245479B (zh) | 一种含有乳双歧杆菌发酵活性提取物的面膜 | |
| CN104195098A (zh) | 铁皮石斛干细胞及其分离培养方法 | |
| CN104673589A (zh) | 一种辣木保健酒配方及制备方法 | |
| Wu et al. | Optimizing co-culture conditions of adventitious roots of Echinacea pallida and Echinacea purpurea in air-lift bioreactor systems | |
| CN114146133A (zh) | 宽叶苔草在制备具有抗衰老功效产品中的应用 | |
| KR101771695B1 (ko) | 스피루리나 추출물을 유효성분으로 함유하는 비만 예방 및 치료용 약학적 조성물 | |
| CN107198227A (zh) | 一种牛樟芝酵素及其制备方法 | |
| CN102771857A (zh) | 通过液体深层发酵制备的松茸保健饮料及其制备方法 | |
| CN110004065A (zh) | 一种红褐灵芝新菌种及其人工栽培方法和应用 | |
| KR101819060B1 (ko) | 황련해독탕 발효물을 유효성분으로 포함하는 화장료 조성물 | |
| CN109364073A (zh) | 一种荜茇酰胺生物碱在制备治疗白癜风中促进黑色素生成的药物中的用途 | |
| KR20120059258A (ko) | 생물 활성을 가지는 한방식재료 추출액 및 이의 제조방법 | |
| CN110564575A (zh) | 一种护肠胃养颜的铁皮石斛酒及其制作方法 | |
| CN109259223B (zh) | 一种云参酵素加工的方法 | |
| CN107296045A (zh) | 云芝糖肽用于抗病毒的新用途 | |
| CN110810693A (zh) | 一种含有显齿蛇葡萄的饮料组合物及其制备方法 | |
| CN105713841A (zh) | 一种尖孢镰孢及其在苦荞麦种子萌发预处理上的应用 | |
| CN109160947B (zh) | 一种螺旋藻藻蓝蛋白的制备方法及其应用 | |
| CN104911068A (zh) | 一种曼地亚红豆杉保健酒及其制作方法 | |
| CN107736524B (zh) | 一种芹菜全浆乳酸菌饮品及其制备方法 | |
| CN106309613B (zh) | 具有抗炎、抗过敏作用的中药及其制备方法 | |
| CN104946460B (zh) | 一种曼地亚红豆杉奶味酒饮料及其制作方法 | |
| CN114146040B (zh) | 一种强化皮肤屏障、抗衰老的组合物及其制备方法和应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |