CN114097922A - High-solubility soybean polypeptide and preparation process thereof - Google Patents

High-solubility soybean polypeptide and preparation process thereof Download PDF

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Publication number
CN114097922A
CN114097922A CN202111516310.5A CN202111516310A CN114097922A CN 114097922 A CN114097922 A CN 114097922A CN 202111516310 A CN202111516310 A CN 202111516310A CN 114097922 A CN114097922 A CN 114097922A
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protein isolate
soybean protein
stirring
soybean
mixing
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赵媛媛
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Shandong Xinran Pharmaceutical Technology Co ltd
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Shandong Xinran Pharmaceutical Technology Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/14Vegetable proteins
    • A23J3/16Vegetable proteins from soybean
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/30Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A23J3/346Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/66Proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/18Peptides; Protein hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention provides high-solubility soybean polypeptide, which belongs to the field of soybean protein processing. The invention has mature process, can ensure the purity of the soybean polypeptide through multiple times of filtration, and can ensure the dissolution efficiency so as to ensure the stability of absorption; the soybean polypeptide prepared by the invention is easy to digest and absorb; because of high solubility, the artificial synthesis solves the digestion problem in vitro, and the artificial synthesis can be directly absorbed without being digested in the human body; meanwhile, due to the antioxidation, the free radicals of the human body are eliminated, the formation of new free radicals is prevented, and the process of the degeneration and the aging of the human body is delayed.

Description

High-solubility soybean polypeptide and preparation process thereof
Technical Field
The invention belongs to the technical field of soybean protein processing, and particularly relates to a high-solubility soybean polypeptide and a preparation process thereof.
Background
Soybean protein: namely, the content of protein contained in the soybean products is about 38% or more, which is 4 to 5 times of that of the cereal food. Soy protein is a vegetable protein. The soybean protein is a plant protein, the amino acid composition of the soybean protein is similar to that of milk protein, except for slightly lower methionine, the content of other essential amino acids is rich, and the soybean protein is a complete plant protein.
The soybean polypeptide and the preparation process thereof in the prior art are widely applied.
However, the existing soybean polypeptide and the preparation process thereof have the problems of insufficient nutrition, backward preparation process steps and low purity.
Therefore, the invention of highly soluble soybean polypeptide is necessary.
Disclosure of Invention
In order to solve the above technical problems, the present invention provides a highly soluble soybean polypeptide, wherein the present invention is realized by the following steps:
the high-solubility soybean polypeptide mainly comprises the following raw materials: soybean protein isolate and distilled water, wherein the soybean protein isolate is 1-10 parts, and the distilled water is 25-30 parts.
The preparation process of the high-solubility soybean polypeptide specifically comprises the following steps:
the method comprises the following steps: extracting and purifying the soybean protein isolate, taking out the soybean protein isolate, filtering through a stainless steel mesh screen to remove redundant impurities and fine particles, stirring the soybean protein isolate, cooling, and mixing and stirring after treatment;
step two: mixing and stirring the soybean protein isolate, putting the soybean protein isolate into a stainless steel mixing and sterilizing barrel, adding distilled water, mixing and stirring by a stirrer, standing again after mixing and stirring, floating a floating layer, standing again, and standing at room temperature to prepare soybean protein isolate raw pulp slurry;
step three: filtering the soybean protein isolate primary pulp slurry, namely placing the soybean protein isolate primary pulp slurry in a vibrating mesh screen, performing vibrating screening to remove redundant mixed impurities and mixed particles, stirring again, standing after stirring and mixing, and preparing for heating treatment;
step four: heating soybean protein isolate primary pulp slurry, namely putting the soybean protein isolate primary pulp slurry into a heating pot for heating and stirring continuously, adding alkaline enzyme for enzymolysis after heating and heating, and filtering and retaining liquid after enzymolysis;
step five: a residual liquid treatment processing procedure, namely filtering the solution by a roll-type ultrafiltration membrane purification system with the molecular weight cutoff of 1500-2000Da to obtain a trapped liquid, and transferring the trapped liquid into a fermentation tank for fermentation to obtain a neutral protease fermentation liquid; adding a flocculating agent into the neutral protease fermentation liquor to obtain neutral protease filtrate; centrifuging, coarse filtering, fine filtering, adding trehalose and beta-cyclodextrin, stirring, collecting precipitate, and vacuum drying to obtain neutral protease solid powder;
step six: and (3) storing and retaining the neutral protease solid powder, placing the neutral protease solid powder in a ventilated and dried stainless steel tank, sealing and storing, shading and ventilating.
Compared with the prior art, the invention has the following beneficial effects: the preparation process of the high-solubility soybean polypeptide is widely applied to the technical field of soybean protein processing. The invention has mature process, can ensure the purity of the soybean polypeptide through multiple times of filtration, and can ensure the dissolution efficiency so as to ensure the stability of absorption; the soybean polypeptide prepared by the invention is easy to digest and absorb; because of high solubility, the artificial synthesis solves the digestion problem in vitro, and the artificial synthesis can be directly absorbed without being digested in the human body; simultaneously, due to the antioxidation, the free radicals of the human body are eliminated, the formation of new free radicals is prevented, and the process of the degeneration and the aging of the human body is delayed; therefore, the invention can apply the nutritional characteristics of the soybean polypeptide to health care products such as hypoallergenic food, sports drink, antihypertensive food, fatigue recovery food and the like, and meet the requirements of different people.
Drawings
FIG. 1 is a flow chart of a process for preparing highly soluble soy polypeptides.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
in the figure:
as shown in figure 1
The preparation process of the high-solubility soybean polypeptide specifically comprises the following steps:
s101: extracting and purifying the soybean protein isolate, taking out the soybean protein isolate, filtering through a stainless steel mesh screen to remove redundant impurities and fine particles, stirring the soybean protein isolate, cooling, and mixing and stirring after treatment;
s102: mixing and stirring the soybean protein isolate, putting the soybean protein isolate into a stainless steel mixing and sterilizing barrel, adding distilled water, mixing and stirring by a stirrer, standing again after mixing and stirring, floating a floating layer, standing again, and standing at room temperature to prepare soybean protein isolate raw pulp slurry;
s103: filtering the soybean protein isolate primary pulp slurry, namely placing the soybean protein isolate primary pulp slurry in a vibrating mesh screen, performing vibrating screening to remove redundant mixed impurities and mixed particles, stirring again, standing after stirring and mixing, and preparing for heating treatment;
s104: heating soybean protein isolate primary pulp slurry, namely putting the soybean protein isolate primary pulp slurry into a heating pot for heating and stirring continuously, adding alkaline enzyme for enzymolysis after heating and heating, and filtering and retaining liquid after enzymolysis;
s105: a residual liquid treatment processing procedure, namely filtering the solution by a roll-type ultrafiltration membrane purification system with the molecular weight cutoff of 1500-2000Da to obtain a trapped liquid, and transferring the trapped liquid into a fermentation tank for fermentation to obtain a neutral protease fermentation liquid; adding a flocculating agent into the neutral protease fermentation liquor to obtain neutral protease filtrate; centrifuging, coarse filtering, fine filtering, adding trehalose and beta-cyclodextrin, stirring, collecting precipitate, and vacuum drying to obtain neutral protease solid powder;
s106: and (3) storing and retaining the neutral protease solid powder, placing the neutral protease solid powder in a ventilated and dried stainless steel tank, sealing and storing, shading and ventilating.
Preferably, in S101, the stainless steel mesh screen is a stainless steel mesh screen with a hole diameter of 2-4 mm.
Preferably, in S102, the mixing and stirring speed is set to 350-450 rpm, the standing is divided into two times, each time of the standing is set to 45-50 minutes, and the room temperature is set to 20-25 ℃.
Preferably, in S103, the vibration sieve is a stainless steel vibration sieve with vibration times of 100 and 150 times/min, and the standing time is set to 30-35 min.
Preferably, in S104, the warming temperature is set to 60-65 ℃, and the enzymolysis time is set to 20-25 minutes.
Preferably, in S106, the stainless steel tank has a volume of 500-.
Detailed description of the preferred embodiment
1. Extracting and purifying the soybean protein isolate, taking out the soybean protein isolate, filtering through a stainless steel mesh screen to remove redundant impurities and fine particles, stirring the soybean protein isolate, cooling, and mixing and stirring after treatment;
2. mixing and stirring the soybean protein isolate, putting the soybean protein isolate into a stainless steel mixing and sterilizing barrel, adding distilled water, mixing and stirring by a stirrer, standing again after mixing and stirring, floating a floating layer, standing again, and standing at room temperature to prepare soybean protein isolate raw pulp slurry;
3. filtering the soybean protein isolate primary pulp slurry, namely placing the soybean protein isolate primary pulp slurry in a vibrating mesh screen, performing vibrating screening to remove redundant mixed impurities and mixed particles, stirring again, standing after stirring and mixing, and preparing for heating treatment;
4. heating soybean protein isolate primary pulp slurry, namely putting the soybean protein isolate primary pulp slurry into a heating pot for heating and stirring continuously, adding alkaline enzyme for enzymolysis after heating and heating, and filtering and retaining liquid after enzymolysis;
5. a residual liquid treatment processing procedure, namely filtering the solution by a roll-type ultrafiltration membrane purification system with the molecular weight cutoff of 1500-2000Da to obtain a trapped liquid, and transferring the trapped liquid into a fermentation tank for fermentation to obtain a neutral protease fermentation liquid; adding a flocculating agent into the neutral protease fermentation liquor to obtain neutral protease filtrate; centrifuging, coarse filtering, fine filtering, adding trehalose and beta-cyclodextrin, stirring, collecting precipitate, and vacuum drying to obtain neutral protease solid powder;
6. and (3) storing and retaining the neutral protease solid powder, placing the neutral protease solid powder in a ventilated and dried stainless steel tank, sealing and storing, shading and ventilating.
The technical solutions of the present invention or similar technical solutions designed by those skilled in the art based on the teachings of the technical solutions of the present invention are all within the scope of the present invention.

Claims (5)

1. The high-solubility soybean polypeptide is characterized by mainly comprising the following raw materials: soybean protein isolate and distilled water, wherein the soybean protein isolate is 1-10 parts, and the distilled water is 25-30 parts.
2. The highly soluble soybean polypeptide of claim 1, wherein the highly soluble soybean polypeptide is prepared by a process comprising the steps of:
the method comprises the following steps: extracting and purifying the soybean protein isolate, taking out the soybean protein isolate, filtering through a stainless steel mesh screen to remove redundant impurities and fine particles, stirring the soybean protein isolate, cooling, and mixing and stirring after treatment;
step two: mixing and stirring the soybean protein isolate, putting the soybean protein isolate into a stainless steel mixing and sterilizing barrel, adding distilled water, mixing and stirring by a stirrer, standing again after mixing and stirring, floating a floating layer, standing again, and standing at room temperature to prepare soybean protein isolate raw pulp slurry;
step three: filtering the soybean protein isolate primary pulp slurry, namely placing the soybean protein isolate primary pulp slurry in a vibrating mesh screen, performing vibrating screening to remove redundant mixed impurities and mixed particles, stirring again, standing after stirring and mixing, and preparing for heating treatment;
step four: heating soybean protein isolate primary pulp slurry, namely putting the soybean protein isolate primary pulp slurry into a heating pot for heating and stirring continuously, adding alkaline enzyme for enzymolysis after heating and heating, and filtering and retaining liquid after enzymolysis;
step five: a residual liquid treatment processing procedure, namely filtering the solution by a roll-type ultrafiltration membrane purification system with the molecular weight cutoff of 1500-2000Da to obtain a trapped liquid, and transferring the trapped liquid into a fermentation tank for fermentation to obtain a neutral protease fermentation liquid; adding a flocculating agent into the neutral protease fermentation liquor to obtain neutral protease filtrate; centrifuging, coarse filtering, fine filtering, adding trehalose and beta-cyclodextrin, stirring, collecting precipitate, and vacuum drying to obtain neutral protease solid powder;
step six: and (3) storing and retaining the neutral protease solid powder, placing the neutral protease solid powder in a ventilated and dried stainless steel tank, sealing and storing, shading and ventilating.
3. The process for preparing highly soluble soybean polypeptide as claimed in claim 2, wherein in the second step, the mixing and stirring speed is set to 350-450 rpm, the standing is divided into two times, each time of the standing is set to 45-50 minutes, and the room temperature is set to 20-25 ℃.
4. The process for preparing highly soluble soybean polypeptide according to claim 2, wherein in step three, the vibration sieve is a stainless steel vibration sieve with vibration frequency of 100 and 150 times/min, and the standing time is set to 30-35 min.
5. The process for preparing soybean polypeptide with high solubility of claim 2, wherein in the fourth step, the heating temperature is set to 60-65 ℃ and the enzymolysis time is set to 20-25 minutes.
CN202111516310.5A 2021-12-06 2021-12-06 High-solubility soybean polypeptide and preparation process thereof Pending CN114097922A (en)

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CN202111516310.5A CN114097922A (en) 2021-12-06 2021-12-06 High-solubility soybean polypeptide and preparation process thereof

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Application Number Priority Date Filing Date Title
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CN114097922A true CN114097922A (en) 2022-03-01

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101982087A (en) * 2010-10-28 2011-03-02 天津实发中科百奥工业生物技术有限公司 Method for producing functional soy peptide by alcohol leaching soy protein concentrate
CN102511648A (en) * 2011-12-28 2012-06-27 天津滨海诺奥酶工程技术有限公司 Method for producing soybean polypeptide powder
CN103518944A (en) * 2013-10-17 2014-01-22 丹阳市正大油脂有限公司 Preparation method of soybean peptides
CN108157583A (en) * 2017-12-28 2018-06-15 武汉天天好生物制品有限公司 A kind of highly dissoluble soya-bean polypeptides and its preparation process and application

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101982087A (en) * 2010-10-28 2011-03-02 天津实发中科百奥工业生物技术有限公司 Method for producing functional soy peptide by alcohol leaching soy protein concentrate
CN102511648A (en) * 2011-12-28 2012-06-27 天津滨海诺奥酶工程技术有限公司 Method for producing soybean polypeptide powder
CN103518944A (en) * 2013-10-17 2014-01-22 丹阳市正大油脂有限公司 Preparation method of soybean peptides
CN108157583A (en) * 2017-12-28 2018-06-15 武汉天天好生物制品有限公司 A kind of highly dissoluble soya-bean polypeptides and its preparation process and application

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