CN102511648A - Method for producing soybean polypeptide powder - Google Patents
Method for producing soybean polypeptide powder Download PDFInfo
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- CN102511648A CN102511648A CN2011104453488A CN201110445348A CN102511648A CN 102511648 A CN102511648 A CN 102511648A CN 2011104453488 A CN2011104453488 A CN 2011104453488A CN 201110445348 A CN201110445348 A CN 201110445348A CN 102511648 A CN102511648 A CN 102511648A
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Abstract
The invention relates to a method for processing soybean isolated protein by utilizing a biological enzyme technology. The method comprises the following steps of: during preparation, selecting high-quality soy protein isolate, and dissolving; performing thermal denaturation; adjusting the pH value to 8.0 to 9.0; adding composite hydrolase of alkali protease, neutral protease, papain and trypsin, and reacting for 2 to 3 hours; adjusting the pH value to 4.0 to 6.0; inactivating enzyme; when cooling to a temperature below 50 DEG C, adding filter aid enzyme; collecting filtrate to dissolve filter residues; adding composite hydrolase of acidic protease, neutral protease, papain and trypsin, and reacting for 1 to 2 hours; inactivating the enzyme; when cooling to temperature of below 50 DEG C, adding the filter aid enzyme; filtering by a paperboard; performing ultrafiltration; drying by microwaves under vacuum; and crushing and sieving. The soybean polypeptide powder prepared by the method can improve the solubility of albumen powder to a maximum limit and keep the color and luster of appearance, mouthfeel, flavors and the bioactivity of active ingredients, offers a good mouthfeel, is easy to absorb and can be widely applied to industry of dairy products, drinks and health-care products.
Description
(1) technical field:
The present invention relates to utilize biological enzyme technology to handle the method for soybean protein isolate; The degree of hydrolysis of protein can reach 90%; This method utilizes the filter aid enzyme technology obviously to solve the rate of filtration problem of producing in particular; The soyabean polypeptide powder that utilizes this method to produce more helps absorption of human body, has further improved its nutritive value, and can be used in industries such as dairy products, beverage and health products.
(2) background technology:
Soybean protein isolate, be a kind of be raw material through the food additives of the high protein content that obtains after the further deep processings of technology such as lixiviate, separation with the low temperature desolventizing dregs of beans.Its protein content is higher than 90%; Nutritious; Can be applied in dairy products, bread product and the meat product, be used for improving flavours in food products, improve nutritive value etc., but because still there is the defective of aspects such as solubility property, frothing capacity in soybean protein isolate.Therefore, can utilize soybean protein isolate to be raw material, utilize enzymolysis to get method, further improve its functional characteristic, it is good to obtain dissolubility, is easier to the functional soy peptide of absorption of human body.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing biological enzyme technology to handle soybean protein isolate; It can overcome the problem that exists in the prior art; Both to greatest extent soybean protein isolate is carried out hydrolysis, kept the biologically active of its appearance luster, taste flavor and active ingredient again.
Technical scheme of the present invention: a kind of method of utilizing biological enzyme technology to handle soybean protein isolate is characterized in that it realizes through following step:
(1) dissolving of soybean protein isolate: soybean protein isolate: water=1: 15-1: 20;
(2) thermal denaturation: control temperature heat treatment 30-60min in 80-95 ℃ of scope;
(3) adjusting of Ph: regulate Ph to 8.0-9.0 with natrium carbonicum calcinatum;
(4) under enzymolysis: the 50-55 ℃ condition, add compound protease and carry out first step hydrolysis;
(5) adjusting of Ph: regulate Ph to 4.0-6.0 with citric acid;
(6) enzyme that goes out: heat treatment 5-10min more than 90 ℃
(7) enzyme-addedly help filter: after treating that temperature is cooled to below 50 ℃, the filter aid enzyme that adds 0.1-0.3% helps filter;
(8) plate-frame filtering: collect clear liquid and residue;
(9) dissolving of filter residue: filter residue: water=1: 1-1: 2
(10) secondary enzymolysis:, add compound protease and carry out second hydrolysis in the filter residue amount;
(11) enzyme that goes out: heat treatment 5-10min more than 90 ℃
(12) enzyme-addedly help filter: after treating that temperature is cooled to below 50 ℃, in the filter residue amount, the filter aid enzyme that adds 0.1-0.3% helps filter;
(13) plate-frame filtering: in the clear liquid after collecting clear liquid and adding first step hydrolysis;
(14) ultrafiltration: enzymolysis liquid obtains the 20-30Brix inspissated juice through ultrafiltration;
(15) micro-wave vacuum: the micro-wave vacuum condition is-0.090MPa is to-0.098MPa, and baking temperature is 30-50 ℃, drying time 10-30min;
(16) crushing screening: the soya-bean polypeptides that microwave drying obtains is pulverized through pulverizer, crosses 100 order vibratory sieves.
(17) can: the albumen powder powder after sieving carries out can, obtains soyabean polypeptide powder;
Adding complex enzyme in the above-mentioned steps (4) is main component with the alkali protease; Other compositions also comprise in neutral proteinase, papain, the trypsase a kind of, two kinds or three kinds; Addition is counted 0.3%-0.5% with raw material weight, and hydrolysis temperature 50-55 ℃, enzymolysis time 2-3h;
Adding filter aid enzyme in the above-mentioned steps (7) (12) is main component with trypsase, and other compositions also comprise one or both in phytase, the lipase, and addition is counted 0.1-0.3% with raw material weight;
Adding complex enzyme in the above-mentioned steps (10) is main component with the acid protease; Other compositions also comprise in neutral proteinase, papain, the trypsase a kind of, two kinds or three kinds; Addition is counted 0.3%-0.5% with filter residue weight, and hydrolysis temperature 50-55 ℃, enzymolysis time 1-2h;
Adopt in the above-mentioned steps (15) micro-wave vacuum to remove moisture and reach drying purpose, the micro-wave vacuum condition be-0.090MPa extremely-0.098MPa, baking temperature is 30-50 ℃, drying time 10-30min;
A kind of soyabean polypeptide powder is characterized in that: outward appearance is the faint yellow solid powder, has the peculiar fragrance of certain bean product, and the protein content of product is more than 75%.
Superiority of the present invention is: the technology of 1, producing soyabean polypeptide powder is advanced, and production efficiency is high; 2, produce the product quality height, have no chemical residue, steady quality, its physical state is given agreeable to the taste sense; 3, can effectively utilize the motionless problem of filtration that occurs on the biological enzyme technology process for producing; The biologically active that 4, can keep appearance luster, taste flavor and the active ingredient of product; 5, improved the nutritive value of soya-bean polypeptides, and the soyabean polypeptide powder mouthfeel of producing is easier to absorption of human body well.
(4) specific embodiment:
Embodiment 1:
A kind of method of utilizing biological enzyme technology to handle soybean protein isolate is characterized in that it realizes through following step:
(1) dissolving of soybean protein isolate: soybean protein isolate: water=1: 15;
(2) thermal denaturation: control temperature heat treatment 30min in 80 ℃ of scopes;
(3) adjusting of Ph: regulate Ph to 8.0 with natrium carbonicum calcinatum;
(4) enzymolysis: under 50 ℃ of conditions, add 0.3% compound protein enzyme hydrolysis 2h;
(5) adjusting of Ph: regulate Ph to 4.0 with citric acid;
(6) enzyme that goes out: heat treatment 5min more than 90 ℃
(7) enzyme-addedly help filter: after treating that temperature is cooled to below 50 ℃, add 0.1% filter aid enzyme and help filter;
(8) plate-frame filtering: collect clear liquid and residue;
(9) dissolving of filter residue: filter residue: water=1: 1
(10) secondary enzymolysis:, add 0.3% compound protein enzyme hydrolysis 1h in the filter residue amount;
(11) enzyme that goes out: heat treatment 5min more than 90 ℃
(12) enzyme-addedly help filter: after treating that temperature is cooled to below 50 ℃,, add 0.1% filter aid enzyme and help filter in the filter residue amount;
(13) plate-frame filtering: in the clear liquid after collecting clear liquid and adding first step hydrolysis;
(14) ultrafiltration: enzymolysis liquid obtains the 20Brix inspissated juice through ultrafiltration;
(15) micro-wave vacuum: the micro-wave vacuum condition is-0.090MPa, and baking temperature is 30 ℃, drying time 10min;
(16) crushing screening: the soya-bean polypeptides that microwave drying obtains is pulverized through pulverizer, crosses 100 order vibratory sieves.
(17) can: the albumen powder powder after sieving carries out sterile filling, obtains soyabean polypeptide powder;
Add complex enzyme formulation in the above-mentioned steps (4) and mixed and formed by alkali protease, neutral proteinase, papain, trypsase, its weight ratio is an alkali protease: neutral proteinase: papain: trypsase=6: 2: 1: 2;
Add filter aid enzyme in the above-mentioned steps (7) (12) and mixed and formed by trypsase, phytase, lipase, its weight ratio is a trypsase: phytase: lipase=5: 2: 3;
Add complex enzyme in the above-mentioned steps (10) and mixed and formed by acid protease, neutral proteinase, papain, trypsase, its weight ratio is an acid protease: neutral proteinase: papain: trypsase=7: 3: 1: 2;
A kind of soyabean polypeptide powder is characterized in that: outward appearance is the faint yellow solid powder, has the peculiar fragrance of certain bean product, and the protein content of product is 81%.
Embodiment 2:
A kind of method of utilizing biological enzyme technology to handle soybean protein isolate is characterized in that it realizes through following step:
(1) dissolving of soybean protein isolate: soybean protein isolate: water=1: 20;
(2) thermal denaturation: control temperature heat treatment 60min in 90 ℃ of scopes;
(3) adjusting of Ph: regulate Ph to 9.0 with natrium carbonicum calcinatum;
(4) enzymolysis: under 50 ℃ of conditions, add 0.5% compound protein enzyme hydrolysis 3h;
(5) adjusting of Ph: regulate Ph to 6.0 with citric acid;
(6) enzyme that goes out: heat treatment 10min more than 90 ℃
(7) enzyme-addedly help filter: after treating that temperature is cooled to below 50 ℃, add 0.2% filter aid enzyme and help filter;
(8) plate-frame filtering: collect clear liquid and residue;
(9) dissolving of filter residue: filter residue: water=1: 2
(10) secondary enzymolysis:, add 0.5% compound protein enzyme hydrolysis 2h in the filter residue amount;
(11) enzyme that goes out: heat treatment 10min more than 90 ℃
(12) enzyme-addedly help filter: after treating that temperature is cooled to below 50 ℃,, add 0.2% filter aid enzyme and help filter in the filter residue amount;
(13) plate-frame filtering: in the clear liquid after collecting clear liquid and adding first step hydrolysis;
(14) ultrafiltration: enzymolysis liquid obtains the 30Brix inspissated juice through ultrafiltration;
(15) micro-wave vacuum: the micro-wave vacuum condition is-0.098MPa, and baking temperature is 30 ℃, drying time 30min;
(16) crushing screening: the soya-bean polypeptides that microwave drying obtains is pulverized through pulverizer, crosses 100 order vibratory sieves.
(17) can: the albumen powder powder after sieving carries out sterile filling, obtains soyabean polypeptide powder;
Add complex enzyme formulation in the above-mentioned steps (4) and mixed and formed by alkali protease, neutral proteinase, papain, trypsase, its weight ratio is an alkali protease: neutral proteinase: papain: trypsase=7: 3: 1: 2;
Add filter aid enzyme in the above-mentioned steps (7) (12) and mixed and formed by trypsase, phytase, lipase, its weight ratio is a trypsase: phytase: lipase=6: 3: 1;
Add complex enzyme in the above-mentioned steps (10) and mixed and formed by acid protease, neutral proteinase, papain, trypsase, its weight ratio is an acid protease: neutral proteinase: papain: trypsase=6: 2: 1: 2;
A kind of soyabean polypeptide powder is characterized in that: outward appearance is the faint yellow solid powder, has the peculiar fragrance of certain bean product, and the protein content of product is 83%.
Claims (10)
1. method of utilizing biological enzyme technology to handle soybean protein isolate is characterized in that it realizes through following step:
(1) dissolving of soybean protein isolate: soybean protein isolate: water=1: 15-1: 20;
(2) thermal denaturation: control temperature heat treatment 30-60min in 80-95 ℃ of scope;
(3) adjusting of Ph: regulate Ph to 8.0-9.0 with natrium carbonicum calcinatum;
(4) under enzymolysis: the 50-55 ℃ condition, add compound protease and carry out first step hydrolysis;
(5) adjusting of Ph: regulate Ph to 4.0-6.0 with citric acid;
(6) enzyme that goes out: heat treatment 5-10min more than 90 ℃
(7) enzyme-addedly help filter: after treating that temperature is cooled to below 50 ℃, the filter aid enzyme that adds 0.1-0.3% helps filter;
(8) plate-frame filtering: collect clear liquid and residue;
(9) dissolving of filter residue: filter residue: water=1: 1-1: 2
(10) secondary enzymolysis:, add compound protease and carry out second hydrolysis in the filter residue amount;
(11) enzyme that goes out: heat treatment 5-10min more than 90 ℃
(12) enzyme-addedly help filter: after treating that temperature is cooled to below 50 ℃, in the filter residue amount, the filter aid enzyme that adds 0.1-0.3% helps filter;
(13) plate-frame filtering: in the clear liquid after collecting clear liquid and adding first step hydrolysis;
(14) ultrafiltration: enzymolysis liquid obtains the 20-30Brix inspissated juice through ultrafiltration;
(15) micro-wave vacuum: the micro-wave vacuum condition is-0.090MPa is to-0.098MPa, and baking temperature is 30-50 ℃, drying time 10-30min;
(16) crushing screening: the soya-bean polypeptides that microwave drying obtains is pulverized through pulverizer, crosses 100 order vibratory sieves.
(17) can: the albumen powder powder after sieving carries out sterile filling, obtains soyabean polypeptide powder;
2. a kind of method of utilizing biological enzyme technology to handle soybean protein isolate according to claim 1; It is characterized in that: adding complex enzyme in the step (4) is main component with the alkali protease; Other compositions also comprise in neutral proteinase, papain, the trypsase a kind of, two kinds or three kinds; Addition is counted 0.3%-0.5% with raw material weight, and hydrolysis temperature 50-55 ℃, enzymolysis time 2-3h;
3. a kind of method of utilizing biological enzyme technology to handle soybean protein isolate according to claim 1; It is characterized in that: adding filter aid enzyme in the step (7) (12) is main component with trypsase; Other compositions also comprise one or both in phytase, the lipase, and addition is counted 0.1-0.3% with raw material weight;
4. a kind of method of utilizing biological enzyme technology to handle soybean protein isolate according to claim 1; It is characterized in that: adding complex enzyme in the step (10) is main component with the acid protease; Other compositions also comprise in neutral proteinase, papain, the trypsase a kind of, two kinds or three kinds; Addition is counted 0.3%-0.5% with filter residue weight, and hydrolysis temperature 50-55 ℃, enzymolysis time 1-2h;
5. a kind of method of utilizing biological enzyme technology to handle soybean protein isolate according to claim 2; It is characterized in that: add complex enzyme formulation in the step (4) and mixed and formed by alkali protease, neutral proteinase, papain, trypsase, its weight ratio is an alkali protease: neutral proteinase: papain: trypsase=6: 2: 1: 2;
6. a kind of method of utilizing biological enzyme technology to handle soybean protein isolate according to claim 2; It is characterized in that: add complex enzyme formulation in the step (4) and mixed and formed by alkali protease, neutral proteinase, papain, trypsase, its weight ratio is an alkali protease: neutral proteinase: papain: trypsase=7: 3: 1: 2;
7. a kind of method of utilizing biological enzyme technology to handle soybean protein isolate according to claim 3; It is characterized in that: add filter aid enzyme in the step (7) (12) and mixed and formed by trypsase, phytase, lipase, its weight ratio is a trypsase: phytase: lipase=6: 3: 1
8. a kind of method of utilizing biological enzyme technology to handle soybean protein isolate according to claim 3; It is characterized in that: adding filter aid enzyme in the step (7) (12) is main component with trypsase; Other compositions also comprise one or both in phytase, the lipase, and addition is counted 0.1-0.3% with raw material weight;
9. a kind of method of utilizing biological enzyme technology to handle soybean protein isolate according to claim 4; It is characterized in that: add complex enzyme in the step (10) and mixed and formed by acid protease, neutral proteinase, papain, trypsase, its weight ratio is an acid protease: neutral proteinase: papain: trypsase=7: 3: 1: 2;
10. a kind of method of utilizing biological enzyme technology to handle soybean protein isolate according to claim 4; It is characterized in that: add complex enzyme in the step (10) and mixed and formed by acid protease, neutral proteinase, papain, trypsase, its weight ratio is an acid protease: neutral proteinase: papain: trypsase=6: 2: 1: 2;
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