CN103937863A - Method for improving enzymolysis effect of soybean protein concentrate powder by means of irradiation technology - Google Patents
Method for improving enzymolysis effect of soybean protein concentrate powder by means of irradiation technology Download PDFInfo
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Abstract
The invention belongs to the technical field of development of antioxidant peptides by means of comprehensive utilization of soybean protein, and discloses a method for improving the enzymolysis effect of soybean protein concentrate powder by means of an irradiation technology. A process route comprising the treatment processes of microwave drying, sieving, fast neutron electron beam irradiation, enzymolysis, centrifuging, ultrafiltration and the like is designed preferably through single factor and response surface designs by taking soybean protein concentrate powder as a raw material and taking the hydrolysis degree and the DPPH (1,1-Diphenyl-2-picrylhydrazyl radical) removing rate of ultrafiltrate as balance indexes. After the irradiated soybean protein concentrate powder is subjected to enzymolysis under a proper condition through Alcause protease for 4 hours, the hydrolysis degree is increased from 37.10 percent to over 50.64 percent, and the DPPH removing rate of enzymolysis ultrafiltrate is increased from 18.92 percent to over 25.15 percent. By adopting the method, a certain earlier-stage technical foundation is laid for the research and industrial popularization of development of antioxidant peptides by means of comprehensive utilization of soybean protein resources.
Description
Technical field
The invention belongs to the technical field of soybean protein comprehensive utilization exploitation antioxidation active peptides, relate to a kind of method that improves the concentrated albumen-powder of soybeans hydrolysis result by irradiation technique, taking the concentrated albumen-powder of soybeans as raw material, design one through microwave drying, sieve, electron beam irradiation, enzymolysis, centrifugal, the operational path of ultra-filtration process, can realize by the concentrated albumen-powder of soybeans after radiation treatment through Alcause proteolytic enzyme enzymolysis 4h under its suitable condition, degree of hydrolysis is increased to more than 50.64% by 37.10%, the DPPH clearance rate of enzymolysis ultrafiltrated is increased to more than 25.15% by 18.92%.
Background technology
Soybean protein is a kind of aboundresources and colory vegetable protein, is one of source of current mankind's quality protein.Soybean nutritional composition is very abundant, and in numerous vegetable proteins, nutritive value is the highest.Its protein content is 2.5～8 times of Cereal and potato group foods, and except carbohydrate is lower, other nutritive ingredient is as fat, calcium, phosphorus, iron and vitamins B
1, vitamins B
2deng needed by human nutritive substance, all apparently higher than cereal and potato group food, be a kind of desirable quality plant albumen food.Soybean protein is except methionine(Met) is lower slightly, and the necessary aminoacids content of all the other human bodies is abundant, also approaches most human amino acid on gene structure, is the vegetalitas complete protein of tool nutrition.
The soybean peptides that soybean protein hydrolysis generates, has different physiological roles.Soybean peptides refers to the product that soybean protein hydrolysis obtains, and its amino acid composition is just the same with soybean protein, necessary amino acid reasonable ratio, utilization easily absorbed by the body, but there is the processing characteristics more superior than soybean protein and functional performance.Soybean peptides is a kind of active polypeptide, there is free from beany flavor, without protein denaturation, acid do not precipitate, do not heat solidify, the good processing characteristics such as soluble in water, good fluidity, it is good protective foods material, and have be easy to digested, can antifatigue, the characteristic such as physical strength reinforcing, also contain some physiologically active substance, in body, have different physiological roles.
Irradiation is applied to the sterilising treatment in food conventionally, and the application in enzymolysis process not yet occurs.Irradiation technique is to utilize X ray, gamma-rays or electron beam to carry out ionizing rays to material, to reach objects such as delaying breathing, Shelf-life, Pesticidal and sterilizing, control parasitic infection, quarantine processing.The more traditional food-processing product of irradiation products has its original feature: suitable machining at low temperature, production efficiency are high, operate continuously strong, keep quality, safe, technique is simple, cost is low, less energy consumption, meets the trend of following environmental protection.Irradiation is applied to the sterilising treatment in food conventionally, and the application in enzymolysis process not yet occurs.The present invention utilizes irradiation technique to carry out different radiation treatment to the concentrated albumen-powder of soybeans, has protected the gordian technique of the impact of different irradiation doses on the concentrated albumen-powder of soybeans hydrolysis effect and enzymolysis product soybean peptides DPPH clearance rate.
Degree of hydrolysis is one of index of weighing hydrolysis result, and pH-stat method is current the most widely used protein degree measuring method.Degree of hydrolysis is to weigh peptide bond digested disconnected number, and different enzymes and the avidity of albumen, to approach ability different, also just different to the degree of hydrolysis of albumen, therefore, can use the index of degree of hydrolysis as measurement hydrolysis result.PH-stat method is simple to operate, and test-results accuracy rate is high.PH-stat measuring method: be transferred in enzymatic vessel after adding appropriate distilled water to dissolve appropriate the concentrated albumen-powder of soybeans.After heating protein denaturation, be cooled to optimal temperature, and regulate the pH value of substrate to reach Appropriate.Add subsequently proteolytic enzyme, enzymolysis process remains homo(io)thermism, and the working concentration NaOH that is 1M or the HCl titration of 1M, keeps the variation of pH value in suitable scope, hydrolysis finishes acid or the alkali number that in the whole reaction process of rear mensuration, titration consumes, and the calculation formula of degree of hydrolysis is:
Wherein: the consumption (mL) of V-acid or alkali; The volumetric molar concentration (mol/L) of C-acid or alkali; Mp-protein total mass (g); h
totthe gram equivalent of peptide bond (albumen is got 8.38meqv/g) in-every gram protein; The average dissociation degree of α-amino.
DPPH clearance rate can characterize the intensity of resistance of oxidation, is one of index of weighing soybean peptides resistance of oxidation.DPPH method was suggested in 1958, was widely used in the resistance of oxidation of quantitative assay biological material and food.This method is to have single electron according to DPPH free radical, has the last one to absorb at 517nm place, and its alcoholic solution is the characteristic of purple.In the time having free-radical scavengers to exist, owing to its absorption being faded away with its single electron pairing, its fading extent becomes quantitative relationship with the electron amount of its acceptance, thereby available spectrophotometer carries out quantitative analysis fast.DPPH free radical scavenging activity method adopts 96 orifice plates, add successively the DPPH methanol solution of 100 μ L enzymolysis solutions, 100 μ L methyl alcohol and 100 μ L0.6mM, 96 orifice plates are placed on micro-oscillator and shake 1min, reaction solution is mixed, lucifuge reaction 30min under room temperature, measures its absorbance A at 515nm place by microplate reader
s.Under the same terms, as blank, measure its absorbance A with 100 μ L methyl alcohol replacement enzymolysis solutions
b.The calculation formula of DPPH clearance rate is as follows:
Wherein: A
b: the absorbancy of 200 μ L methyl alcohol and 100 μ L DPPH methanol solutions; A
s: the absorbancy of 100 μ L enzymolysis solutions, 100 μ L methyl alcohol and 100 μ L DPPH methanol solutions.
The technical superiority of this patent---innovatively by irradiation technique processed soybeans concentrated protein powder, initiate real genus with the technology that improves its hydrolysis result.At present, the research of the enzymolysis of biologically active peptides being prepared to effect and physiologically active about irradiation technique have not been reported, patent utilization irradiation technique of the present invention carries out different radiation treatment to the concentrated albumen-powder of soybeans, impact with research irradiation dose on the concentrated albumen-powder of soybeans hydrolysis effect and enzymolysis product Soybean Peptide DPPH clearance rate, initiate real genus.
Summary of the invention
This patent technical issues that need to address:
Taking the concentrated albumen-powder of soybeans as raw material, taking the DPPH clearance rate of degree of hydrolysis and ultrafiltrated as measurement index, by single factor and response surface design, design microwave drying, sieve, the operational path of electron beam irradiation, enzymolysis, centrifugal, ultrafiltration, the concentrated albumen-powder of soybeans of realization after radiation treatment is through Alcause proteolytic enzyme enzymolysis 4h under its suitable condition, degree of hydrolysis is increased to more than 50.64% by 37.10%, and the DPPH clearance rate of enzymolysis ultrafiltrated is increased to more than 25.15% by 18.92%.
The technical scheme of this patent:
1. one kind is improved the method for the concentrated albumen-powder of soybeans hydrolysis result by irradiation technique, it is characterized in that, taking the concentrated albumen-powder of soybeans of protein content 65～85% as raw material, through microwave drying, sieve, electron beam irradiation, enzymolysis, centrifugal, ultra-filtration process, make soybean protein concentrate irradiation dry powder under Alcause proteolytic enzyme suitable condition during by enzymolysis 4h, degree of hydrolysis is increased to more than 50.64% by 37.10%, and the DPPH clearance rate of ultrafiltrated is increased to more than 25.15% by 18.92%;
1) described microwave drying process, that the concentrated albumen-powder of soybeans of protein content 65～85% is placed in to microwave dryer, microwave power is set as 500～800W, and the microwave drying time is set as 5～15min, makes moisture content and be 1～3% soybean protein concentrate dry powder;
2) the described process of sieving, is by the soybean protein concentrate dry powder after microwave drying, excessively for subsequent use after 200～400 mesh sieve;
3) described electron beam irradiation process, be that the soybean protein concentrate dry powder after sieving is sub-packed in to every bag of 100～1000g, put into travelling belt and be conveyed into electron beam irradiation chamber, irradiation dose is 9kGy～140kGy, line speed is set as 5～20m/h, makes soybean protein concentrate irradiation dry powder;
4) described enzymolysis process, taking soybean protein concentrate irradiation dry powder as substrate, after being adapted to concentration of substrate and being 0.5～2.0% according to weight ratio water, through the processing of high speed dispersion clarifixator, setting its rotating speed is that 2000～4000r/min, treatment time are 5～20min; Be placed in again the water-bath heat denatured 15min of 95 DEG C, cooling rear controlled enzymatic hydrolysis temperature is 40～60 DEG C, and regulating pH value is 9.5～11.0, presses [E]/[S] and is 2～6% and add Alcause proteolytic enzyme, the pH value that regulates enzymolysis solution with the NaOH solution of 1M, changes in ± 0.04 it; Under suitable condition, after enzymolysis 4h, the water-bath that enzymolysis solution is placed in to 95 DEG C heats the enzyme 5～15min that goes out, and obtains the enzymolysis solution of soybean protein concentrate irradiation dry powder, learns that after testing the degree of hydrolysis of soybean protein concentrate irradiation dry powder is increased to more than 50.64% by 37.10%;
5) described centrifugal process, is that the enzymolysis solution of soybean protein concentrate irradiation dry powder is carried out to centrifugal treating, and centrifuging temperature is that 2～8 DEG C, rotating speed are 6000～14000r/min, and centrifugation time is 5～20min, collects centrifuged supernatant;
6) described ultra-filtration process, that centrifuged supernatant is controlled under the temperature condition of 10～25 DEG C, carry out total reflux uf processing, the molecular weight cut-off of controlling ultra-filtration membrane is that 1k～3k Da, ultrafiltration flow velocity are that 2～5mL/min, ultrafiltration pressure are 0.1～0.3MPa, collect liquid under ultra-filtration membrane, detect its DPPH clearance rate and be increased to more than 25.15% by 18.92%.
2. a kind of method that improves the concentrated albumen-powder of soybeans hydrolysis result by irradiation technique according to claim 1, is characterized in that, described enzymolysis process required water is any one in selection pure water, deionized water, infiltration water and distilled water.
3. a kind of method that improves the concentrated albumen-powder of soybeans hydrolysis result by irradiation technique according to claim 1, it is characterized in that, described Alcause proteolytic enzyme is to select any one in liquid enzymes or solid enzyme, and requiring the enzyme scope of living is 1,000,000～4,000,000 u/g.
Technique effect of the present invention:
(1) the present invention is taking the concentrated albumen-powder of soybeans as raw material, taking the DPPH clearance rate of degree of hydrolysis and ultrafiltrated as measurement index, by single factor and response surface design, design microwave drying, sieve, the operational path of electron beam irradiation, enzymolysis, centrifugal, ultrafiltration, realize the concentrated albumen-powder of soybeans after radiation treatment, by the Alcause enzyme of liquid or solid under optimum conditions in enzymolysis 4h, degree of hydrolysis can be increased to more than 50.64% by 37.10%, and ultrafiltrated DPPH clearance rate can be increased to more than 25.15% by 18.92%.
(2) the production technology route of the present invention's design is simple and direct, and facility investment is few, and value of the product is high, and industrialization promotion is feasible, is conducive to promote the research of soybean protein resource comprehensive utilization exploitation anti-oxidation peptide.
Embodiment
Embodiment 1:
The concentrated albumen-powder of soybeans of protein content 75% is placed in to microwave dryer, microwave power is set as 750W, the microwave drying time is set as 10min, make moisture content and be 1.0% soybean protein concentrate dry powder, after crossing 325 mesh sieve, be sub-packed in every bag of 100g, put into travelling belt and be conveyed into electron beam irradiation chamber, irradiation dose is 9kGy, line speed is set as 5m/h, makes soybean protein concentrate irradiation dry powder; Then again taking soybean protein concentrate irradiation dry powder as substrate, after being adapted to concentration of substrate and being 1.7% with pure water according to weight ratio, through the processing of high speed dispersion clarifixator, setting its rotating speed is that 3700r/min, treatment time are 5min; Be placed in the water-bath heat denatured 15min of 95 DEG C, cooling rear controlled enzymatic hydrolysis temperature is 60 DEG C again, and regulating pH value is 9.8, is 3% to add the Alcause proteolytic enzyme of liquid (enzyme work scope is 1,000,000 u/g by [E]/[S].), use the NaOH solution of 1M to regulate the pH value of enzymolysis solution, it is changed in ± 0.04; Under suitable condition, after enzymolysis 4h, the water-bath that enzymolysis solution is placed in to 95 DEG C heats the enzyme 5min that goes out, and obtains the enzymolysis solution of soybean protein concentrate irradiation dry powder, learns that after testing the degree of hydrolysis of soybean protein concentrate irradiation dry powder is increased to more than 50.64% by 37.10%; Enzymolysis solution is carried out to centrifugal treating, and centrifuging temperature is that 6 DEG C, rotating speed are 12000r/min, and centrifugation time is 10min, collects centrifuged supernatant; Centrifuged supernatant is controlled under the temperature condition of 20 DEG C, carry out total reflux uf processing, the molecular weight cut-off of controlling ultra-filtration membrane is that 1k Da, ultrafiltration flow velocity are that 2mL/min, ultrafiltration pressure are 0.3MPa, collects liquid under ultra-filtration membrane, detects its DPPH clearance rate and is increased to more than 25.15% by 18.92%.
Embodiment 2:
The concentrated albumen-powder of soybeans of protein content 85% is placed in to microwave dryer, microwave power is set as 500W, the microwave drying time is set as 15min, make moisture content and be 2.6% soybean protein concentrate dry powder, after crossing 250 mesh sieve, be sub-packed in every bag of 500g, put into travelling belt and be conveyed into electron beam irradiation chamber, irradiation dose is 36.8kGy, line speed is set as 20m/h, makes soybean protein concentrate irradiation dry powder; Then again taking soybean protein concentrate irradiation dry powder as substrate, after being adapted to concentration of substrate and being 2.0% with deionized water according to weight ratio, through the processing of high speed dispersion clarifixator, setting its rotating speed is that 2000r/min, treatment time are 20min; Be placed in the water-bath heat denatured 15min of 80 DEG C, cooling rear controlled enzymatic hydrolysis temperature is 53 DEG C again, and regulating pH value is 11.1, is 5% to add the Alcause proteolytic enzyme of solid (enzyme work scope is 4,000,000 u/g by [E]/[S].), use the NaOH solution of 1M to regulate the pH value of enzymolysis solution, it is changed in ± 0.04; Under suitable condition, after enzymolysis 4h, the water-bath that enzymolysis solution is placed in to 95 DEG C heats the enzyme 11min that goes out, and obtains the enzymolysis solution of soybean protein concentrate irradiation dry powder, learns that after testing the degree of hydrolysis of soybean protein concentrate irradiation dry powder is increased to more than 50.64% by 37.10%; Enzymolysis solution is carried out to centrifugal treating, and centrifuging temperature is that 2 DEG C, rotating speed are 8000r/min, and centrifugation time is 20min, collects centrifuged supernatant; Centrifuged supernatant is controlled under the temperature condition of 10 DEG C, carry out total reflux uf processing, the molecular weight cut-off of controlling ultra-filtration membrane is that 3k Da, ultrafiltration flow velocity are that 4mL/min, ultrafiltration pressure are 0.2MPa, collects liquid under ultra-filtration membrane, detects its DPPH clearance rate and is increased to more than 25.15% by 18.92%.
Embodiment 3:
The concentrated albumen-powder of soybeans of protein content 65% is placed in to microwave dryer, microwave power is set as 800W, the microwave drying time is set as 5min, make moisture content and be 1.5% soybean protein concentrate dry powder, after crossing 200 mesh sieve, be sub-packed in every bag of 1000g, put into travelling belt and be conveyed into electron beam irradiation chamber, irradiation dose is 65kGy, line speed is set as 15m/h, makes soybean protein concentrate irradiation dry powder; Then again taking soybean protein concentrate irradiation dry powder as substrate, after being adapted to concentration of substrate and being 1.4% according to weight ratio water infiltration water, through the processing of high speed dispersion clarifixator, setting its rotating speed is that 3400r/min, treatment time are 14min; Be placed in the water-bath heat denatured 15min of 92 DEG C, cooling rear controlled enzymatic hydrolysis temperature is 47 DEG C again, and regulating pH value is 11.0, is 4% to add the Alcause proteolytic enzyme of liquid (enzyme work scope is 2,000,000 u/g by [E]/[S].), use the NaOH solution of 1M to regulate the pH value of enzymolysis solution, it is changed in ± 0.04; Under suitable condition, after enzymolysis 4h, the water-bath that enzymolysis solution is placed in to 95 DEG C heats the enzyme 15min that goes out, and obtains the enzymolysis solution of soybean protein concentrate irradiation dry powder, learns that after testing the degree of hydrolysis of soybean protein concentrate irradiation dry powder is increased to more than 50.64% by 37.10%; Enzymolysis solution is carried out to centrifugal treating, and centrifuging temperature is that 8 DEG C, rotating speed are 6000r/min, and centrifugation time is 5min, collects centrifuged supernatant; Centrifuged supernatant is controlled under the temperature condition of 20 DEG C, carry out total reflux uf processing, the molecular weight cut-off of controlling ultra-filtration membrane is that 2k Da, ultrafiltration flow velocity are that 3mL/min, ultrafiltration pressure are 0.1MPa, collects liquid under ultra-filtration membrane, detects its DPPH clearance rate and is increased to more than 25.15% by 18.92%.
Embodiment 4:
The concentrated albumen-powder of soybeans of protein content 80% is placed in to microwave dryer, microwave power is set as 650W, the microwave drying time is set as 14min, make moisture content and be 1.2% soybean protein concentrate dry powder, after crossing 400 mesh sieve, be sub-packed in every bag of 800g, put into travelling belt and be conveyed into electron beam irradiation chamber, irradiation dose is 108.7kGy, line speed is set as 10m/h, makes soybean protein concentrate irradiation dry powder; Then again taking soybean protein concentrate irradiation dry powder as substrate, after being adapted to concentration of substrate and being 0.8% according to weight ratio water distilled water, through the processing of high speed dispersion clarifixator, setting its rotating speed is that 3000r/min, treatment time are 8min; Be placed in the water-bath heat denatured 15min of 89 DEG C, cooling rear controlled enzymatic hydrolysis temperature is 57 DEG C again, and regulating pH value is 10.7, is 2% to add the Alcause proteolytic enzyme of solid (enzyme work scope is 1,000,000 u/g by [E]/[S].), use the NaOH solution of 1M to regulate the pH value of enzymolysis solution, it is changed in ± 0.04; Under suitable condition, after enzymolysis 4h, the water-bath that enzymolysis solution is placed in to 95 DEG C heats the enzyme 7min that goes out, and obtains the enzymolysis solution of soybean protein concentrate irradiation dry powder, learns that after testing the degree of hydrolysis of soybean protein concentrate irradiation dry powder is increased to more than 50.64% by 37.10%; Enzymolysis solution is carried out to centrifugal treating, and centrifuging temperature is that 2 DEG C, rotating speed are 14000r/min, and centrifugation time is 15min, collects centrifuged supernatant; Centrifuged supernatant is controlled under the temperature condition of 10 DEG C, carry out total reflux uf processing, the molecular weight cut-off of controlling ultra-filtration membrane is that 3k Da, ultrafiltration flow velocity are that 5mL/min, ultrafiltration pressure are 0.2MPa, collects liquid under ultra-filtration membrane, detects its DPPH clearance rate and is increased to more than 25.15% by 18.92%.
Embodiment 5:
The concentrated albumen-powder of soybeans of protein content 75% is placed in to microwave dryer, microwave power is set as 700W, the microwave drying time is set as 8min, make moisture content and be 2.3% soybean protein concentrate dry powder, after crossing 270 mesh sieve, be sub-packed in every bag of 400g, put into travelling belt and be conveyed into electron beam irradiation chamber, irradiation dose is 135kGy, line speed is set as 8m/h, makes soybean protein concentrate irradiation dry powder; Then again taking soybean protein concentrate irradiation dry powder as substrate, after being adapted to concentration of substrate and being 0.5% according to weight ratio water pure water, through the processing of high speed dispersion clarifixator, setting its rotating speed is that 4000r/min, treatment time are 11min; Be placed in the water-bath heat denatured 15min of 86 DEG C, cooling rear controlled enzymatic hydrolysis temperature is 50 DEG C again, and regulating pH value is 10.4, is 6% to add the Alcause proteolytic enzyme of liquid (enzyme work scope is 4,000,000 u/g by [E]/[S].), use the NaOH solution of 1M to regulate the pH value of enzymolysis solution, it is changed in ± 0.04; Under suitable condition, after enzymolysis 4h, the water-bath that enzymolysis solution is placed in to 95 DEG C heats the enzyme 9min that goes out, and obtains the enzymolysis solution of soybean protein concentrate irradiation dry powder, learns that after testing the degree of hydrolysis of soybean protein concentrate irradiation dry powder is increased to more than 50.64% by 37.10%; Enzymolysis solution is carried out to centrifugal treating, and centrifuging temperature is that 4 DEG C, rotating speed are 10000r/min, and centrifugation time is 10min, collects centrifuged supernatant; Centrifuged supernatant is controlled under the temperature condition of 15 DEG C, carry out total reflux uf processing, the molecular weight cut-off of controlling ultra-filtration membrane is that 2k Da, ultrafiltration flow velocity are that 4mL/min, ultrafiltration pressure are 0.1MPa, collects liquid under ultra-filtration membrane, detects its DPPH clearance rate and is increased to more than 25.15% by 18.92%.
Embodiment 6:
The concentrated albumen-powder of soybeans of protein content 70% is placed in to microwave dryer, microwave power is set as 550W, the microwave drying time is set as 12min, make moisture content and be 3% soybean protein concentrate dry powder, after crossing 200 mesh sieve, be sub-packed in every bag of 600g, put into travelling belt and be conveyed into electron beam irradiation chamber, irradiation dose is 138.7kGy, line speed is set as 12m/h, makes soybean protein concentrate irradiation dry powder; Then again taking soybean protein concentrate irradiation dry powder as substrate, after being adapted to concentration of substrate and being 1.1% according to weight ratio water distilled water, through the processing of high speed dispersion clarifixator, setting its rotating speed is that 2600r/min, treatment time are 17min; Be placed in the water-bath heat denatured 15min of 83 DEG C, cooling rear controlled enzymatic hydrolysis temperature is 40 DEG C again, and regulating pH value is 9.5, is 5% to add the Alcause proteolytic enzyme of solid (enzyme work scope is 3,000,000 u/g by [E]/[S].), use the NaOH solution of 1M to regulate the pH value of enzymolysis solution, it is changed in ± 0.04; Under suitable condition, after enzymolysis 4h, the water-bath that enzymolysis solution is placed in to 95 DEG C heats the enzyme 13min that goes out, and obtains the enzymolysis solution of soybean protein concentrate irradiation dry powder, learns that after testing the degree of hydrolysis of soybean protein concentrate irradiation dry powder is increased to more than 50.64% by 37.10%; Enzymolysis solution is carried out to centrifugal treating, and centrifuging temperature is that 6 DEG C, rotating speed are 8000r/min, and centrifugation time is 15min, collects centrifuged supernatant; Centrifuged supernatant is controlled under the temperature condition of 25 DEG C, carry out total reflux uf processing, the molecular weight cut-off of controlling ultra-filtration membrane is that 1k Da, ultrafiltration flow velocity are that 2mL/min, ultrafiltration pressure are 0.3MPa, collects liquid under ultra-filtration membrane, detects its DPPH clearance rate and is increased to more than 25.15% by 18.92%.
Claims (3)
1. one kind is improved the method for the concentrated albumen-powder of soybeans hydrolysis result by irradiation technique, it is characterized in that, taking the concentrated albumen-powder of soybeans of protein content 65%～85% as raw material, through microwave drying, sieve, electron beam irradiation, enzymolysis, centrifugal, ultra-filtration process, make soybean protein concentrate irradiation dry powder under Alcause proteolytic enzyme suitable condition during by enzymolysis 4h, degree of hydrolysis is increased to more than 50.64% by 37.10%, and the DPPH clearance rate of ultrafiltrated is increased to more than 25.15% by 18.92%;
1) described microwave drying process, that the concentrated albumen-powder of soybeans of protein content 65%～85% is placed in to microwave dryer, microwave power is set as 500～800W, and the microwave drying time is set as 5～15min, makes moisture content and be 1%～3% soybean protein concentrate dry powder;
2) the described process of sieving, is by the soybean protein concentrate dry powder after microwave drying, excessively for subsequent use after 200～400 mesh sieve;
3) described electron beam irradiation process, be that the soybean protein concentrate dry powder after sieving is sub-packed in to every bag of 100～1000g, put into travelling belt and be conveyed into electron beam irradiation chamber, irradiation dose is 9kGy～140kGy, line speed is set as 5～20m/h, makes soybean protein concentrate irradiation dry powder;
4) described enzymolysis process, taking soybean protein concentrate irradiation dry powder as substrate, after being adapted to concentration of substrate and being 0.5%～2.0% according to weight ratio water, through the processing of high speed dispersion clarifixator, setting its rotating speed is that 2000～4000r/min, treatment time are 5～20min; Be placed in again the water-bath heat denatured 15min of 95 DEG C, cooling rear controlled enzymatic hydrolysis temperature is 40～60 DEG C, and regulating pH value is 9.5～11.0, presses [E]/[S] and is 2%～6% and add Alcause proteolytic enzyme, the pH value that regulates enzymolysis solution with the NaOH solution of 1M, changes in ± 0.04 it; Under suitable condition, after enzymolysis 4h, the water-bath that enzymolysis solution is placed in to 95 DEG C heats the enzyme 5～15min that goes out, and obtains the enzymolysis solution of soybean protein concentrate irradiation dry powder, learns that after testing the degree of hydrolysis of soybean protein concentrate irradiation dry powder is increased to more than 50.64% by 37.10%;
5) described centrifugal process, is that the enzymolysis solution of soybean protein concentrate irradiation dry powder is carried out to centrifugal treating, and centrifuging temperature is that 2～8 DEG C, rotating speed are 6000～14000r/min, and centrifugation time is 5～20min, collects centrifuged supernatant;
6) described ultra-filtration process, that centrifuged supernatant is controlled under the temperature condition of 10～25 DEG C, carry out total reflux uf processing, the molecular weight cut-off of controlling ultra-filtration membrane is that 1k～3k Da, ultrafiltration flow velocity are that 2～5mL/min, ultrafiltration pressure are 0.1～0.3MPa, collect liquid under ultra-filtration membrane, detect its DPPH clearance rate and be increased to more than 25.15% by 18.92%.
2. a kind of method that improves the concentrated albumen-powder of soybeans hydrolysis result by irradiation technique according to claim 1, is characterized in that, described enzymolysis process required water is any one in pure water, deionized water, infiltration water and distilled water.
3. a kind of method that improves the concentrated albumen-powder of soybeans hydrolysis result by irradiation technique according to claim 1, it is characterized in that, described Alcause proteolytic enzyme is any one in liquid enzymes or solid enzyme, and requiring the enzyme scope of living is 1,000,000～4,000,000 u/g.
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| CN106854672A (en) * | 2017-01-04 | 2017-06-16 | 江南大学 | A kind of method that electron beam irradiation joint enzyme process isolates and purifies the wheat germ polypeptide of affine calcium high |
| CN109182431A (en) * | 2018-09-17 | 2019-01-11 | 东北农业大学 | A kind of method that soybean protein isolate is modified |
| CN109182431B (en) * | 2018-09-17 | 2022-01-04 | 东北农业大学 | Method for modifying soybean protein isolate |
| CN110250435A (en) * | 2019-05-27 | 2019-09-20 | 黑龙江省北大荒绿色健康食品有限责任公司 | A kind of production method of functional instant bean powder |
| WO2023285685A1 (en) * | 2021-07-16 | 2023-01-19 | Société des Produits Nestlé S.A. | Method of making a powder egg analogue |
| CN116046516A (en) * | 2023-04-03 | 2023-05-02 | 四川中科高能科技发展有限责任公司 | Food quality improving method based on irradiation parameter automatic screening |
| CN116046516B (en) * | 2023-04-03 | 2023-06-09 | 四川中科高能科技发展有限责任公司 | Food quality improving method based on irradiation parameter automatic screening |
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