CN101982087A - Method for producing functional soy peptide by alcohol leaching soy protein concentrate - Google Patents
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Abstract
The invention relates to a method for producing functional soy peptide by alcohol leaching soy protein concentrate, which prepares soy peptide by a two-stage protease hydrolysis process. The method comprises the following main steps: hydrolyzing and preprocessing feed solution cellulose; carrying out instantaneous pretreatment at ultra temperature; hydrolyzing protease; carrying out pH regulation and centrifugal separation; carrying out ultrafiltration separation; and condensing and drying. In the process, the raw material of alcohol leaching soy protein concentrate is performed with cellulose hydrolysis pretreatment and instantaneous pretreatment at ultra temperature, and overcomes the deficiencies of poor dissolubility of alcohol leaching soy protein concentrate and low total nitrogen content of the product; enzymolysis liquid pH value is regulated twice to precipitate protein, and two-stage enzymolysis process control is carried out; and while saving energy, the produced soy peptide product has good taste and high quality. The invention provides a new way for the deep processing of the alcohol leaching soy protein concentrate. The product of the invention has obvious practical effect and is suitable for industrial production.
Description
Technical field
The invention belongs to the deep processing field of soybean prod; Relate to a kind of method of producing soya-bean polypeptides, particularly a kind of method of utilizing alcohol processed soybean protein concentrate to produce functional soy peptide.
Background technology
Along with the continuous development of food science and technology, the exploitation of soybean deep processed product is also moved towards deeply gradually, and it is an important directions of soybean deep processing that soybean protein hydrolysis is produced soya-bean polypeptides.Soya-bean polypeptides not only has good nutritive peculiarity, also has physiological and pharmacological effect widely and than soybean protein rich functions characteristic more, is a kind of very promising functional food ingredient.The research and development of soya-bean polypeptides have become the focus of emerging in the world high-tech biological field, have great market potential.
Soybean protein isolate, FSPC can be as the raw materials of soya-bean polypeptides production.FSPC is to remove the non-protein ingredient of low molecular weight soluble from defatted soybean meal, mainly is soluble sugar, ash content and various odour components etc., finally makes the protein contents on dry basis be no less than 65% soy protein products.The production method of FSPC is divided into diluted acid precipitation acid system (abbreviation acid system) and ethanol washing method (being called for short pure method).At present, many in the world with pure method production FSPC, account for more than 60%.Compare to soybean protein isolate, the FSPC product yield height (60%～65%) that pure method is produced, relative low price, production process produce the three wastes hardly, and advantages for development are obvious, meet national environmental protection policy.And soybean protein isolate produces problems such as serious environmental pollution in process of production, does not meet China's strategy of sustainable development, will progressively be eliminated.Utilize alcohol processed soybean protein concentrate to produce soya-bean polypeptides, can overcome the environmental issue in the soybean protein isolate production process on the one hand, carried out useful exploration for the added value of utilizing new way and raising alcohol processed soybean protein concentrate of exploitation alcohol processed soybean protein concentrate on the other hand, can effectively prolong the industrial chain of alcohol processed soybean protein concentrate manufacturing enterprise, and increase under the situation of too many equipment need not, significantly improve the performance of enterprises.
But it should be noted that, protein majority sex change in the FSPC that the alcohol method is produced, poorly soluble, be difficult to carry out the depth hydrolysis, protein content in the FSPC is relatively low simultaneously, produce soya-bean polypeptides with it and be difficult to improve content of total nitrogen in the product, the existence of these problems has increased the difficulty of utilizing alcohol processed soybean protein concentrate to produce soya-bean polypeptides.Therefore, provide the method that a kind of technology is simple, obvious results is utilized alcohol processed soybean protein concentrate production functional soy peptide, become one of these those skilled in the art problem anxious to be addressed.
Summary of the invention
The objective of the invention is to overcome the above-mentioned weak point of directly utilizing in the alcohol processed soybean protein concentrate enzymolysis production soya-bean polypeptides process, developing a kind of very low alcohol processed soybean protein concentrate of nitrogen soluble index (NSI) that utilizes is the functional soya-bean polypeptides product of having of raw material production; A kind of method of utilizing alcohol processed soybean protein concentrate to produce functional soy peptide is provided.
For achieving the above object, implementation of the present invention is as follows:
A kind of method of utilizing alcohol processed soybean protein concentrate to produce functional soy peptide is characterized in that implementation step is as follows:
(1) material liquid preparation
Get 1 part of alcohol processed soybean protein concentrate by weight and 6-12 part distilled water mixes, form uniform FSPC slurries through stirring;
(2) cellulose hydrolyzation preliminary treatment
With weight content is that the pH value that the hydrochloric acid solution of 1-20% is regulated the FSPC slurries is 4-6, the adding cellulase, and enzyme concentration is the 0.5%-2% of alcohol processed soybean protein concentrate raw material weight, stirs under 40-50 ℃ of condition enzymolysis 2-3 hour; The cellulase treatment fluid after under the 2000-5000 rev/min of condition centrifugal 5-10 minute, is removed clear liquid, and add the distilled water with the clear liquid equal volume, stirring becomes the first pretreatment fluid of FSPC;
(3) the instantaneous preliminary treatment of superhigh temperature
The first pretreatment fluid of FSPC is carried out the instantaneous preliminary treatment of superhigh temperature, and inlet temperature is 110-150 ℃, and the processing time is 3-15 second, is cooled to 55-70 ℃ through cooler and obtains FSPC secondary pretreatment fluid;
(4) two-stage enzyme digestion reaction
FSPC secondary pretreatment fluid enters enzymatic vessel by the road, regulating its temperature is 55-65 ℃, with weight content is that the sodium hydrate regulator solution pH value of 1-20% is 7.5-8.5, add alkali protease, dosage is the 0.5-2% of alcohol processed soybean protein concentrate raw material weight, add neutral proteinase and food flavor enzyme after enzymolysis 15-60 minute, the dosage of two kinds of enzymes is the 0.2-1.0% of alcohol processed soybean protein concentrate raw material weight, processing time is 2-4 hour, obtain enzymolysis liquid after being cooled to 20-30 ℃, ceaselessly stir in the enzymolysis process;
(5) twice pH regulate and centrifugation
With weight content is that the pH value that the hydrochloric acid solution of 1-20% is regulated enzymolysis liquid is 4.3 and left standstill 1-2 hour under 20-30 ℃ of condition, make alkali protease wherein, neutral proteinase and food flavor enzyme deactivation, and precipitation macro-molecular protein wherein, the enzymolysis liquid that to handle through enzyme-deactivating under 2000-5000 rev/min of condition centrifugal 5-10 minute, discard solid portion, and be that the sodium hydroxide solution regulator solution body portion pH of 1-20% is 6.0-7.0 with weight content once more, left standstill clear liquid after centrifugal 5-10 minute also removal precipitation obtains enzymolysis under the 2000-5000 rev/min of condition 1-2 hour;
(6) ultra-filtration and separation
Is ultrafilter ultrafiltration below the 10000Da with clear liquid behind the enzymolysis through molecular cut off, and the ultrafiltration condition is temperature 30-50 ℃, and pressure is 0.01-0.2Mpa, and ultrafiltration sees through liquid and enters next procedure, and the proteolysis jar that trapped fluid returns step (4) continues enzymolysis;
(7) concentrate with dry
Ultrafiltration sees through liquid and is concentrated into 20-40Bx through vacuum concentrator, the spray-dried soya-bean polypeptides powder that obtains.
Used FSPC raw material is the FSPC raw material that utilizes the ethanol washing method to obtain; Protein content 〉=65%, nitrogen soluble index are 4.0-7.0, moisture≤8%, fat≤0.5%.
The vigor of cellulase is 10000u/g, and the vigor of alkali protease is 2.4Aua/g, and the vigor of neutral proteinase is 0.8Aun/g, and the vigor of food flavor enzyme is 0.8Lapu/g.
Ultra-filtration and separation is to select the hollow-fibre membrane ultrafilter for use, under 0.01-0.2Mpa pressure enzymolysis liquid is carried out ultra-filtration and separation.
Utilize centrifugal spray drying tower during spray-drying, at inlet temperature 150-165 ℃, outlet temperature 60-85 ℃, wind speed 0.3m
3Carry out spray-drying under the condition of/min, thereby make the soya-bean polypeptides powder.
The invention has the beneficial effects as follows:
Pass through pretreatment modes such as cellulose hydrolyzation, the instantaneous preliminary treatment of superhigh temperature among the present invention, make the nitrogen soluble index (NSI) of alcohol processed soybean protein concentrate of sex change improve and remove the glucide of major part, significantly improve the total nitrogen content of product, the hydrolysis of multi-enzyme system has guaranteed that the soya-bean polypeptides product has excellent function and molecular weight distribution characteristic.
It is poorly soluble to have overcome alcohol processed soybean protein concentrate by cellulose hydrolyzation preliminary treatment and the instantaneous preliminary treatment of superhigh temperature in this technology, the deficiency that the product total nitrogen content is low, significantly improved the content of soluble nitrogen in the material liquid, for ensuing enzymolysis has been created good condition; Reconcile the technology controlling and process of enzymolysis liquid pH value protein precipitation and two-stage enzymolysis twice, in energy savings, make that the soya-bean polypeptides product mouthfeel of being produced is good, the quality height, molecular weight distribution is concentrated, for new approach has been opened up in the deep processing of alcohol processed soybean protein concentrate.It is short that another remarkable advantage of the inventive method is exactly the processing time, reduced the possibility of contamination of products.
The specific embodiment
Below in conjunction with embodiment the production method of utilizing alcohol processed soybean protein concentrate to produce soya-bean polypeptides provided by the present invention is further described.
Embodiment 1
A kind of method of utilizing alcohol processed soybean protein concentrate to produce functional soy peptide, concrete implementation step is as follows:
((1) material liquid preparation
Get 1 part of alcohol processed soybean protein concentrate by weight and 6-12 part distilled water mixes, form uniform FSPC slurries through stirring;
(2) cellulose hydrolyzation preliminary treatment
With weight content is that the pH value that the hydrochloric acid solution of 1-20% is regulated the FSPC slurries is 4-6, the adding cellulase, and enzyme concentration is the 0.5%-2% of alcohol processed soybean protein concentrate raw material weight, stirs under 40-50 ℃ of condition enzymolysis 2-3 hour; The cellulase treatment fluid after under the 2000-5000 rev/min of condition centrifugal 5-10 minute, is removed clear liquid, and add the distilled water with the clear liquid equal volume, stirring becomes the first pretreatment fluid of FSPC;
(3) the instantaneous preliminary treatment of superhigh temperature
The first pretreatment fluid of FSPC is carried out the instantaneous preliminary treatment of superhigh temperature, and inlet temperature is 110-150 ℃, and the processing time is 3-15 second, is cooled to 55-70 ℃ through cooler and obtains FSPC secondary pretreatment fluid;
(4) two-stage enzyme digestion reaction
FSPC secondary pretreatment fluid enters enzymatic vessel by the road, regulating its temperature is 55-65 ℃, with weight content is that the sodium hydrate regulator solution pH value of 1-20% is 7.5-8.5, add alkali protease, dosage is the 0.5-2% of alcohol processed soybean protein concentrate raw material weight, add neutral proteinase and food flavor enzyme after enzymolysis 15-60 minute, the dosage of two kinds of enzymes is the 0.2-1.0% of alcohol processed soybean protein concentrate raw material weight, processing time is 2-4 hour, obtain enzymolysis liquid after being cooled to 20-30 ℃, ceaselessly stir in the enzymolysis process;
(5) twice pH regulate and centrifugation
With weight content is that the pH value that the hydrochloric acid solution of 1-20% is regulated enzymolysis liquid is 4.3 and left standstill 1-2 hour under 20-30 ℃ of condition, make alkali protease wherein, neutral proteinase and food flavor enzyme deactivation, and precipitation macro-molecular protein wherein, the enzymolysis liquid that to handle through enzyme-deactivating under 2000-5000 rev/min of condition centrifugal 5-10 minute, discard solid portion, and be that the sodium hydroxide solution regulator solution body portion pH of 1-20% is 6.0-7.0 with weight content once more, left standstill clear liquid after centrifugal 5-10 minute also removal precipitation obtains enzymolysis under the 2000-5000 rev/min of condition 1-2 hour;
(6) ultra-filtration and separation
Is ultrafilter ultrafiltration below the 10000Da with clear liquid behind the enzymolysis through molecular cut off, and the ultrafiltration condition is temperature 30-50 ℃, and pressure is 0.01-0.2Mpa, and ultrafiltration sees through liquid and enters next procedure, and the proteolysis jar that trapped fluid returns step (4) continues enzymolysis;
(7) concentrate with dry
Ultrafiltration sees through liquid and is concentrated into 20-40Bx through vacuum concentrator, the spray-dried soya-bean polypeptides powder that obtains.
Used FSPC raw material is the FSPC raw material that utilizes the ethanol washing method to obtain; Protein content 〉=65%, nitrogen soluble index are 4.0-7.0, moisture≤8%, fat≤0.5%.
The vigor of cellulase is 10000u/g, and the vigor of alkali protease is 2.4Aua/g, and the vigor of neutral proteinase is 0.8Aun/g, and the vigor of food flavor enzyme is 0.8Lapu/g.
Ultra-filtration and separation is to select the hollow-fibre membrane ultrafilter for use, under 0.01-0.2Mpa pressure enzymolysis liquid is carried out ultra-filtration and separation.
Utilize centrifugal spray drying tower during spray-drying, at inlet temperature 150-165 ℃, outlet temperature 60-85 ℃, wind speed 0.3m
3Carry out spray-drying under the condition of/min, thereby make the soya-bean polypeptides powder.
The present invention adopts raw material to be the commercially available prod.
The cellulase that uses in the present invention is the product of Tianjin Noao Sci ﹠ Tech Development Co., Ltd., and alkali protease, neutral proteinase and food flavor enzyme are the product of letter (China) Bioisystech Co., Ltd of Novi; Also can change the enzyme preparation product of other companies into, the addition of various enzymes can convert definite by those skilled in the art according to its vigor difference routinely.
Embodiment 2
(1) material liquid preparation
The alcohol processed soybean protein concentrate 1kg that gets Tianjin Shifaguanhua Biologicgal Science Co., Ltd mixes with the distilled water of 9kg, forms uniform FSPC slurries through stirring;
(2) cellulose hydrolyzation preliminary treatment
With weight content is that the pH value that 10% hydrochloric acid solution is regulated the FSPC slurries is 5.0, the adding cellulase, and enzyme concentration is 1.5% of an alcohol processed soybean protein concentrate raw material weight, stirs, enzymolysis is 2 hours under 45 ℃ of conditions; The cellulase treatment fluid after under 2000 rev/mins of conditions centrifugal 10 minutes, is removed clear liquid, and add the distilled water with the clear liquid equal volume, stirring becomes the first pretreatment fluid of FSPC;
(3) the instantaneous preliminary treatment of superhigh temperature
The first pretreatment fluid of FSPC is carried out the instantaneous preliminary treatment of superhigh temperature, and inlet temperature is 120 ℃, and the processing time is 4 seconds, is cooled to 55 ℃ through cooler and obtains FSPC secondary pretreatment fluid;
(4) two-stage enzyme digestion reaction
FSPC secondary pretreatment fluid enters enzymatic vessel by the road, regulating its temperature is 65 ℃, with weight content is that 8% sodium hydrate regulator solution pH value is 8.0, add alkali protease, dosage is 2% of an alcohol processed soybean protein concentrate raw material weight, and enzymolysis adds neutral proteinase and food flavor enzyme after 15 minutes, the dosage of two kinds of enzymes is 0.3% of alcohol processed soybean protein concentrate raw material weight, processing time is 2 hours, obtains enzymolysis liquid after being cooled to 30 ℃, ceaselessly stirs in the enzymolysis process;
(5) twice pH regulate and centrifugation
With weight content is that the pH value that 10% hydrochloric acid solution is regulated enzymolysis liquid is 4.3 and left standstill 2 hours under 30 ℃ of conditions, make wherein alkali protease, neutral proteinase and food flavor enzyme deactivation, and precipitation macro-molecular protein wherein, the enzymolysis liquid that to handle through enzyme-deactivating under 2000 rev/mins of conditions centrifugal 10 minutes, discard solid portion, and be that 8% sodium hydroxide solution regulator solution body portion pH is 6.5 with weight content once more, left standstill clear liquid after centrifugal 10 minutes also removal precipitations obtain enzymolysis under 2000 rev/mins of conditions 1 hour;
(6) ultra-filtration and separation
Is the ultrafilter ultrafiltration of 10000Da with clear liquid behind the enzymolysis through molecular cut off, and the ultrafiltration condition is 30 ℃ of temperature, and pressure is 0.01Mpa, and ultrafiltration sees through liquid and enters next procedure, and the proteolysis jar that trapped fluid returns step (4) continues enzymolysis;
(7) concentrate with dry
Ultrafiltration sees through liquid and enter centrifugal spray drying tower after vacuum concentrator is concentrated into 35Bx, 150 ℃ of inlet temperatures, and 75 ℃ of outlet temperatures, wind speed 0.3m
3Carry out spray-drying under the condition of/min, thereby make the soya-bean polypeptides powder.
Other are with embodiment 1.
It is 81.3% that soya-bean polypeptides product in this example is measured protein content with triumphant formula nitriding, the product yield is 46%, account for more than 95% of total protein with the gel chromatography determining molecular weight less than the protein of 6000D, molecular weight distribution is comparatively concentrated, and mouthfeel is better.
Embodiment 3
(1) material liquid preparation
The precious 1kg of soymilk that gets benefit sea (Port of Fangcheng) soy industry Co., Ltd mixes with the distilled water of 10kg, forms uniform FSPC slurries through stirring;
(2) cellulose hydrolyzation preliminary treatment
With weight content is that the pH value that 10% hydrochloric acid solution is regulated the FSPC slurries is 5.0, the adding cellulase, and enzyme concentration is 2% of an alcohol processed soybean protein concentrate raw material weight, stirs, enzymolysis is 3 hours under 50 ℃ of conditions; The cellulase treatment fluid after under 5000 rev/mins of conditions centrifugal 6 minutes, is removed clear liquid, and add the distilled water with the clear liquid equal volume, stirring becomes the first pretreatment fluid of FSPC;
(3) the instantaneous preliminary treatment of superhigh temperature
The first pretreatment fluid of FSPC is carried out the instantaneous preliminary treatment of superhigh temperature, and inlet temperature is 140 ℃, and the processing time is 10 seconds, is cooled to 70 ℃ through cooler and obtains FSPC secondary pretreatment fluid;
(4) two-stage enzyme digestion reaction
FSPC secondary pretreatment fluid enters enzymatic vessel by the road, regulating its temperature is 65 ℃, with weight content is that 8% sodium hydrate regulator solution pH value is 8.0, add alkali protease, dosage is 1% of an alcohol processed soybean protein concentrate raw material weight, and enzymolysis adds neutral proteinase and food flavor enzyme after 30 minutes, the dosage of two kinds of enzymes is 0.6% of alcohol processed soybean protein concentrate raw material weight, processing time is 2 hours, obtains enzymolysis liquid after being cooled to 30 ℃, ceaselessly stirs in the enzymolysis process;
(5) twice pH regulate and centrifugation
With weight content is that the pH value that 10% hydrochloric acid solution is regulated enzymolysis liquid is 4.3 and left standstill 2 hours under 30 ℃ of conditions, make wherein alkali protease, neutral proteinase and food flavor enzyme deactivation, and precipitation macro-molecular protein wherein, the enzymolysis liquid that to handle through enzyme-deactivating under 5000 rev/mins of conditions centrifugal 6 minutes, discard solid portion, and be that 8% sodium hydroxide solution regulator solution body portion pH is 6.5 with weight content once more, left standstill clear liquid after centrifugal 6 minutes also removal precipitations obtain enzymolysis under 5000 rev/mins of conditions 2 hours;
(6) ultra-filtration and separation
Is the ultrafilter ultrafiltration of 6000Da with clear liquid behind the enzymolysis through molecular cut off, and the ultrafiltration condition is 30 ℃ of temperature, and pressure is 0.05Mpa, and ultrafiltration sees through liquid and enters next procedure, and the proteolysis jar that trapped fluid returns step (4) continues enzymolysis;
(7) concentrate with dry
Ultrafiltration sees through liquid and enter centrifugal spray drying tower after vacuum concentrator is concentrated into 30Bx, 150 ℃ of inlet temperatures, and 75 ℃ of outlet temperatures, wind speed 0.3m
3Carry out spray-drying under the condition of/min, thereby make the soya-bean polypeptides powder.
Other are with embodiment 1.
It is 86.1% that soya-bean polypeptides product in this example is measured protein content with triumphant formula nitriding, the product yield is 51%, account for more than 90% of total protein with the gel chromatography determining molecular weight less than the protein of 4000D, molecular weight distribution is comparatively concentrated, and mouthfeel is better.
Above-mentioned with reference to the detailed description of embodiment to utilizing alcohol processed soybean protein concentrate production functional soy peptide method to carry out; be illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (5)
1. method of utilizing alcohol processed soybean protein concentrate to produce functional soy peptide is characterized in that implementation step is as follows:
(1) material liquid preparation
Get 1 part of alcohol processed soybean protein concentrate by weight and 6-12 part distilled water mixes, form uniform FSPC slurries through stirring;
(2) cellulose hydrolyzation preliminary treatment
With weight content is that the pH value that the hydrochloric acid solution of 1-20% is regulated the FSPC slurries is 4-6, the adding cellulase, and enzyme concentration is the 0.5%-2% of alcohol processed soybean protein concentrate raw material weight, stirs under 40-50 ℃ of condition enzymolysis 2-3 hour; The cellulase treatment fluid after under the 2000-5000 rev/min of condition centrifugal 5-10 minute, is removed clear liquid, and add the distilled water with the clear liquid equal volume, stirring becomes the first pretreatment fluid of FSPC;
(3) the instantaneous preliminary treatment of superhigh temperature
The first pretreatment fluid of FSPC is carried out the instantaneous preliminary treatment of superhigh temperature, and inlet temperature is 110-150 ℃, and the processing time is 3-15 second, is cooled to 55-70 ℃ through cooler and obtains FSPC secondary pretreatment fluid;
(4) two-stage enzyme digestion reaction
FSPC secondary pretreatment fluid enters enzymatic vessel by the road, regulating its temperature is 55-65 ℃, with weight content is that the sodium hydrate regulator solution pH value of 1-20% is 7.5-8.5, add alkali protease, dosage is the 0.5-2% of alcohol processed soybean protein concentrate raw material weight, add neutral proteinase and food flavor enzyme after enzymolysis 15-60 minute, the dosage of two kinds of enzymes is the 0.2-1.0% of alcohol processed soybean protein concentrate raw material weight, processing time is 2-4 hour, obtain enzymolysis liquid after being cooled to 20-30 ℃, ceaselessly stir in the enzymolysis process;
(5) twice pH regulate and centrifugation
With weight content is that the pH value that the hydrochloric acid solution of 1-20% is regulated enzymolysis liquid is 4.3 and left standstill 1-2 hour under 20-30 ℃ of condition, make alkali protease wherein, neutral proteinase and food flavor enzyme deactivation, and precipitation macro-molecular protein wherein, the enzymolysis liquid that to handle through enzyme-deactivating under 2000-5000 rev/min of condition centrifugal 5-10 minute, discard solid portion, and be that the sodium hydroxide solution regulator solution body portion pH of 1-20% is 6.0-7.0 with weight content once more, left standstill clear liquid after centrifugal 5-10 minute also removal precipitation obtains enzymolysis under the 2000-5000 rev/min of condition 1-2 hour;
(6) ultra-filtration and separation
Is ultrafilter ultrafiltration below the 10000Da with clear liquid behind the enzymolysis through molecular cut off, and the ultrafiltration condition is temperature 30-50 ℃, and pressure is 0.01-0.2Mpa, and ultrafiltration sees through liquid and enters next procedure, and the proteolysis jar that trapped fluid returns step (4) continues enzymolysis;
(7) concentrate with dry
Ultrafiltration sees through liquid and is concentrated into 20-40Bx through vacuum concentrator, the spray-dried soya-bean polypeptides powder that obtains.
2. the method for utilizing alcohol processed soybean protein concentrate to produce functional soy peptide according to claim 1 is characterized in that used FSPC raw material is the FSPC raw material that utilizes the ethanol washing method to obtain; Protein content 〉=65%, nitrogen soluble index are 4.0-7.0, moisture≤8%, fat≤0.5%.
3. the method for utilizing alcohol processed soybean protein concentrate to produce functional soy peptide according to claim 1, the vigor that it is characterized in that cellulase is 10000u/g, the vigor of alkali protease is 2.4Aua/g, the vigor of neutral proteinase is 0.8Aun/g, and the vigor of food flavor enzyme is 0.8Lapu/g.
4. the method for utilizing alcohol processed soybean protein concentrate to produce functional soy peptide according to claim 1 is characterized in that ultra-filtration and separation is to select the hollow-fibre membrane ultrafilter for use, carries out ultra-filtration and separation to enzymolysis liquid under 0.01-0.2Mpa pressure.
5. the method for utilizing alcohol processed soybean protein concentrate to produce functional soy peptide according to claim 1 is utilized centrifugal spray drying tower when it is characterized in that spray-drying, at inlet temperature 150-165 ℃, and outlet temperature 60-85 ℃, wind speed 0.3m
3Carry out spray-drying under the condition of/min, thereby make the soya-bean polypeptides powder.
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| CN102669407A (en) * | 2012-06-06 | 2012-09-19 | 黑龙江省大豆技术开发研究中心 | Method for improving solubility of alcohol leaching soy protein concentrate by use of step-by-step enzymolysis technology |
| CN104719611A (en) * | 2013-12-18 | 2015-06-24 | 中粮营养健康研究院有限公司 | Method for preparing soybean peptides through enzymolysis of soy protein |
| CN104719611B (en) * | 2013-12-18 | 2018-04-03 | 中粮营养健康研究院有限公司 | The method that Soybean Peptide is prepared by enzymatic hydrolysis of soybean albumen |
| CN105685368A (en) * | 2016-01-27 | 2016-06-22 | 山东长润生物有限公司 | Method for producing soybean protein concentrate |
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| CN108157579B (en) * | 2018-01-08 | 2021-07-13 | 恩施德源健康科技发展有限公司 | Preparation method of cardamine violifolia selenium polypeptide with high organic selenium content |
| CN109566853A (en) * | 2018-12-26 | 2019-04-05 | 武汉新华扬生物股份有限公司 | The preparation method of soybean protein isolate and its soybean protein isolate obtained, soybean protein isolate product |
| CN111374223B (en) * | 2018-12-29 | 2023-08-18 | 丰益(上海)生物技术研发中心有限公司 | Preparation method of milk powder oil protein component |
| CN111374223A (en) * | 2018-12-29 | 2020-07-07 | 丰益(上海)生物技术研发中心有限公司 | Preparation method of milk powder oil protein component |
| CN110292111A (en) * | 2019-06-06 | 2019-10-01 | 光泽县泽农生物科技有限公司 | A kind of spraying production technology of chicken small-molecular peptides vacuum concentration |
| CN114680166A (en) * | 2020-12-30 | 2022-07-01 | 丰益(上海)生物技术研发中心有限公司 | Anti-freezing preservative, food containing anti-freezing preservative and food preservation method |
| CN114680166B (en) * | 2020-12-30 | 2024-03-29 | 丰益(上海)生物技术研发中心有限公司 | Antifreeze preservative, food containing antifreeze preservative and food preservation method |
| CN114097922A (en) * | 2021-12-06 | 2022-03-01 | 山东信然医药科技有限公司 | High-solubility soybean polypeptide and preparation process thereof |
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