CN112335888A - Sea cucumber and abalone oligopeptide powder and preparation method thereof - Google Patents
Sea cucumber and abalone oligopeptide powder and preparation method thereof Download PDFInfo
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- CN112335888A CN112335888A CN202011241681.2A CN202011241681A CN112335888A CN 112335888 A CN112335888 A CN 112335888A CN 202011241681 A CN202011241681 A CN 202011241681A CN 112335888 A CN112335888 A CN 112335888A
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Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention provides sea cucumber and abalone oligopeptide powder and a preparation method thereof, belonging to the technical field of health care products and comprising sea cucumber oligopeptide powder, abalone oligopeptide powder, cod collagen peptide powder, oyster oligopeptide powder, sea mussel oligopeptide powder, tussah pupa oligopeptide powder and crude polysaccharide; wherein the total amount of the sea cucumber oligopeptide powder, the abalone oligopeptide powder, the cod collagen peptide powder, the oyster oligopeptide powder, the sea mussel oligopeptide powder and the tussah pupa oligopeptide powder accounts for not less than 90 percent of the total amount of the sea cucumber oligopeptide powder, the abalone oligopeptide powder, the cod collagen peptide powder, the oyster oligopeptide powder, the sea mussel oligopeptide powder, the tussah pupa oligopeptide powder and the crude polysaccharide. The sea cucumber and abalone oligopeptide powder can improve immunity and resist fatigue; preventing tumor and cancer; regulating blood pressure and blood lipid, and has adjuvant treatment effect on diabetes; can delay aging, help sleep, and delay the appearance of senile plaque; tonifying kidney and strengthening body; the radioprotective activity of leukocyte is improved.
Description
Technical Field
The invention relates to the technical field of health products, in particular to stichopus japonicus and abalone oligopeptide powder and a preparation method thereof.
Background
At present, the research and industrialization of domestic peptides are mostly in the research stage. The lower the molecular weight the better the product, the stronger the functionality. And are classified into large polypeptides, small molecule peptides, oligopeptides, and short peptides. However, it has been clear over many years that the function of a peptide product depends primarily on the amino acid sequence and structure, i.e., the potency, of the peptide from which it is composed.
The application of marine polysaccharides in cancer and bone treatment in the American and Japanese polysaccharide products is relatively wide at present, but domestic polysaccharide products still stay in a relatively crude processing stage, most of mixed polysaccharides have insufficient precision, and a general technical bottleneck mainly exists in extraction, screening and other links.
In China, a plurality of enterprises and factories are used for processing aquatic products, and in the production of products, the boiled liquid of marine food materials (sea cucumbers, abalones and shellfish) is mostly discarded. Through research, a large amount of active substances such as water-soluble polysaccharide, polypeptide and the like exist in the boiled liquid, and the boiled liquid is discarded to cause great waste, so that the waste is changed into valuable. The quantity is very large, and the quality of the final product is even better than that of the bulk preparation of the raw materials, so that the solution is realized, and the social benefit and the economic benefit are obvious.
Disclosure of Invention
Aiming at the technical defects, the invention aims to provide sea cucumber and abalone oligopeptide powder and a preparation method thereof.
In order to solve the technical problems, the invention adopts the following technical scheme:
the invention provides sea cucumber-abalone oligopeptide powder, which comprises sea cucumber oligopeptide powder, abalone oligopeptide powder, cod collagen peptide powder, oyster oligopeptide powder, sea mussel oligopeptide powder, tussah pupa oligopeptide powder and crude polysaccharide; wherein the total amount of the sea cucumber oligopeptide powder, the abalone oligopeptide powder, the cod collagen peptide powder, the oyster oligopeptide powder, the sea mussel oligopeptide powder and the tussah pupa oligopeptide powder accounts for not less than 90 percent of the total amount of the sea cucumber oligopeptide powder, the abalone oligopeptide powder, the cod collagen peptide powder, the oyster oligopeptide powder, the sea mussel oligopeptide powder, the tussah pupa oligopeptide powder and the crude polysaccharide.
The invention also provides a preparation method of the sea cucumber and abalone oligopeptide powder, which comprises the following steps:
s1, preparing raw materials, wherein the raw materials comprise sea cucumber oligopeptide powder, abalone oligopeptide powder, cod collagen peptide powder, oyster oligopeptide powder, marine mussel oligopeptide powder, tussah pupa oligopeptide powder and crude polysaccharide;
s2, weighing raw materials, and feeding and mixing the raw materials through a three-dimensional mixer for 60 minutes to obtain a mixture;
s3, filtering the mixture by using a 80-mesh sieve to obtain the sea cucumber and abalone oligopeptide powder.
Preferably, the preparation method of the sea cucumber oligopeptide powder comprises the following steps:
collecting sea cucumber cooking liquor, soaking and foaming water, filtering out sea cucumber tissue fragments, silt and other particulate substances in the water by using a cartridge filter, concentrating and desalting filtrate by using an organic membrane with the aperture of 1-2-ten-thousand Da to obtain inorganic salt concentrated solution, separating silt from particulate solids by using a cyclone separator, and subjecting the sea cucumber fragments to enzymolysis;
mixing the sea cucumber tissue fragments with the concentrated solution, adjusting the solid content of the feed liquid to 10-20%, adjusting the temperature of the feed liquid to 40-55 ℃, adjusting the pH of the feed liquid to 2.5-9.0, and adding special compound protease, wherein the proportion of the compound protease is 2709 alkaline protease, 20 ten thousand mu/g, and 10-40%; 30% -60% of As1398 neutral protease with the concentration of 10 ten thousand mu/g; 10-40% of trypsin, 1.0-10% of flavor enzyme and 1.0-5.0% of total protein, stirring for enzymolysis for 3-6h, adjusting the pH of the enzymolysis solution to 5.0-7.0, heating to 70-75 ℃ for enzyme deactivation, removing granular impurities and lipids by using a tubular centrifuge, cooling the filtrate to 10-30 ℃ through a heat exchanger, separating through an organic membrane with the molecular weight cutoff of 5000-20000 Da, and taking the permeate as oligopeptide;
heating the permeate to 50-80 ℃, adding beta-cyclodextrin, wherein the total protein amount of the beta-cyclodextrin is 1-10%, stirring for 20-60 min, passing the fishy smell-removed liquid through a granular activated carbon column for further fishy smell removal, smell removal and decoloration, passing the column-passed liquid through a 50-100 nm ceramic membrane or cotton cake filter for decarburizing to obtain a fine filtrate, further concentrating the fine filtrate through an organic membrane with the pore diameter of 50Da-100Da until the solid content is 17-18%, then carrying out reduced pressure evaporation concentration until the solid content is more than or equal to 40%, and carrying out spray drying to obtain the sea cucumber oligopeptide powder.
Preferably, the preparation method of the abalone oligopeptide powder comprises the following steps:
collecting abalone cooking liquor and soaking water, filtering out sea cucumber tissue fragments, silt and other particulate matters in the water by using a cartridge filter, concentrating and desalting filtrate by using an organic membrane with the aperture of 1-2-ten-thousand Da to obtain inorganic salt concentrated solution, separating silt from particulate solid matters by using a cyclone separator, and subjecting the abalone fragments to enzymolysis;
mixing the abalone tissue fragments with a concentrated solution, adjusting the solid content of the feed liquid to 10-20%, adjusting the temperature of the feed liquid to 40-55 ℃, adjusting the pH of the feed liquid to 2.5-9.0, and adding special compound protease, wherein the proportion of the compound protease is 2709 alkaline protease, 20 ten thousand mu/g, 10-40%; 30% -60% of As1398 neutral protease with the concentration of 10 ten thousand mu/g; 10-40% of trypsin, 1.0-10% of flavor enzyme and 1.0-5.0% of total protein, stirring for enzymolysis for 3-6h, adjusting the pH of the enzymolysis solution to 5.0-7.0, heating to 70-75 ℃ for enzyme deactivation, removing granular impurities and lipids by using a tubular centrifuge, cooling the filtrate to 10-30 ℃ through a heat exchanger, separating through an organic membrane with the molecular weight cutoff of 5000-20000 Da, and taking the permeate as oligopeptide;
heating the permeate to 50-80 ℃, adding beta-cyclodextrin, wherein the total protein amount of the beta-cyclodextrin is 1-10%, stirring for 20-60 min, passing the fishy smell-removed liquid through a granular activated carbon column for further fishy smell removal, smell removal and decoloration, passing the column-passed liquid through a 50-100 nm ceramic membrane or cotton cake filter for decarburizing to obtain a fine filtrate, further concentrating the fine filtrate through an organic membrane with the pore diameter of 50Da-100Da until the solid content is 17-18%, then carrying out reduced pressure evaporation concentration until the solid content is more than or equal to 40%, and carrying out spray drying to obtain the abalone oligopeptide powder.
Preferably, the preparation method of the cod collagen peptide powder comprises the following steps:
selecting cod skin as a raw material, washing the cod skin with clear water, adding a mixed solution of calcium hydroxide and sodium hydroxide, soaking for 8-14 hours, wherein the concentration of the mixed solution of the calcium hydroxide and the sodium hydroxide is 0.2-0.5%, and the weight ratio of the calcium hydroxide to the sodium hydroxide is 1: 2-4, then repeatedly washing with clear water to make the pH value of the mixture neutral;
adding diluted acid water solution with concentration of 0.3-0.6%, soaking for 1-3 hr, and repeatedly washing with clear water to make pH value neutral;
crushing fish skin, stirring and extracting in hot water of 70-80 ℃ for 8-10 hours, and filtering to remove insoluble substances to obtain collagen extracting solution;
adjusting pH of the collagen extract to neutral, adding enzyme for enzymolysis for 3-5 hr under stirring, and allowing enzymolysis temperature to 40-50 deg.C;
heating to 80 ℃, inactivating enzyme of the enzymolysis liquid for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, wherein the air inlet temperature is 180 ℃, the air outlet temperature is 80 ℃, and obtaining cod collagen peptide powder;
vacuum packaging and low-temperature preservation.
Preferably, the preparation method of the oyster oligopeptide powder comprises the following steps:
preparing oyster powder, namely cleaning, draining, pulping and drying fresh oysters to obtain powder;
mixing the slurry, namely mixing the oyster powder and the purified water in a ratio of 1: 2;
performing enzymolysis, namely performing enzymolysis by using neutral protease, wherein the enzyme adding amount is 1 percent of the protein content of a substrate;
centrifuging, adding 30% liquid alkali to adjust pH to 8.0, centrifuging at 50 deg.C for 4 hr, and collecting supernatant;
membrane filtration, namely removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight less than 5ku, and concentrating by using a nanofiltration membrane until the solid content reaches 10%;
heating to 80 ℃, inactivating enzyme of the enzymolysis liquid for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, wherein the air inlet temperature is 180 ℃, the air outlet temperature is 80 ℃, and obtaining the marine oyster oligopeptide powder;
vacuum packaging and low-temperature preservation.
Preferably, the preparation method of the marine mussel oligopeptide powder comprises the following steps:
preparing marine mussel powder, cleaning fresh marine mussels, draining, pulping, and drying to obtain powder;
mixing pulp, namely mixing the marine mussel powder and the purified water in a ratio of 1: 2;
performing enzymolysis, namely performing enzymolysis by using neutral protease, wherein the enzyme adding amount is 1 percent of the protein content of a substrate;
centrifuging, adding 30% liquid alkali to adjust pH to 8.0, centrifuging at 50 deg.C for 4 hr, and collecting supernatant;
membrane filtration, namely removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight less than 5ku, and concentrating by using a nanofiltration membrane until the solid content reaches 10%;
heating to 80 ℃, inactivating enzyme for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, and obtaining marine mussel oligopeptide powder, wherein the air inlet temperature is 180 ℃, and the air outlet temperature is 80 ℃;
vacuum packaging and low-temperature preservation.
Preferably, the preparation method of the tussah pupa oligopeptide powder comprises the following steps:
preparing tussah pupa powder, namely cleaning, draining, pulping and drying fresh tussah pupas into powder;
mixing pulp, namely mixing the tussah pupa powder and the purified water in a ratio of 1: 2;
performing enzymolysis, namely performing enzymolysis by using neutral protease, wherein the enzyme adding amount is 1 percent of the protein content of a substrate;
centrifuging, adding 30% liquid alkali to adjust pH to 8.0, centrifuging at 50 deg.C for 4 hr, and collecting supernatant;
membrane filtration, namely removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight less than 5ku, and concentrating by using a nanofiltration membrane until the solid content reaches 10%;
heating to 80 ℃, inactivating enzyme for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, and obtaining tussah pupa oligopeptide powder, wherein the air inlet temperature is 180 ℃, and the air outlet temperature is 80 ℃;
vacuum packaging and low-temperature preservation.
The invention has the beneficial effects that: the sea cucumber protein contains more than 18 kinds of amino acids, and the composition ratio of the amino acids is better. The sea cucumber contains 0.4mg/100g of vanadium, and the physiological function of the vanadium is to transmit iron in a body to the liver, so that the iron can be fully absorbed to form hemoglobin. Contains chondroitin sulfate, Stichopus japonicus mucopolysaccharide, etc. Chondroitin sulfate can delay human body aging, and Stichopus japonicus mucopolysaccharide has broad-spectrum anticancer effect, and can prevent acute radiation injury and promote hemopoiesis recovery.
The small molecular oligopeptide is absorbed more quickly and easily by organisms than single amino acid, and has more important biological activity in the aspect of human metabolism. Therefore, the sea cucumber oligopeptide after enzymolysis has higher biological value and more important physiological functions. The sea cucumber peptide is degraded by enzyme, has no antigenicity, and does not cause any anaphylactic reaction after being eaten, thus having high safety. Improving immunity and resisting fatigue; preventing tumor and cancer; regulating blood pressure and blood lipid, and has adjuvant treatment effect on diabetes; delaying aging, helping sleep, and delaying the appearance of senile plaque; tonifying kidney and strengthening body; radioprotective enhances the primary function of phagocytic activity of leukocytes.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a flow chart of a preparation method of sea cucumber-abalone oligopeptide powder provided by the embodiment of the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The sea cucumber and abalone oligopeptide powder comprises the following components in percentage by weight:
15% of sea cucumber oligopeptide powder, 15% of abalone oligopeptide powder, 15% of cod collagen peptide powder, 15% of oyster oligopeptide powder, 15% of sea mussel oligopeptide powder, 15% of tussah pupa oligopeptide powder and 10% of crude polysaccharide.
Example 2
16% of sea cucumber oligopeptide powder, 14% of abalone oligopeptide powder, 15% of cod collagen peptide powder, 15% of oyster oligopeptide powder, 15% of sea mussel oligopeptide powder, 18% of tussah pupa oligopeptide powder and 7% of crude polysaccharide.
Example 3
18% of sea cucumber oligopeptide powder, 14% of abalone oligopeptide powder, 15% of cod collagen peptide powder, 16% of oyster oligopeptide powder, 17% of sea mussel oligopeptide powder, 15% of tussah pupa oligopeptide powder and 5% of crude polysaccharide.
As shown in fig. 1, the method for preparing sea cucumber and abalone oligopeptide powder of embodiments 1 to 3 comprises the following steps:
s1, preparing raw materials, wherein the raw materials comprise sea cucumber oligopeptide powder, abalone oligopeptide powder, cod collagen peptide powder, oyster oligopeptide powder, marine mussel oligopeptide powder, tussah pupa oligopeptide powder and crude polysaccharide;
s2, weighing raw materials, and feeding and mixing the raw materials through a three-dimensional mixer for 60 minutes to obtain a mixture;
s3, filtering the mixture by using a 80-mesh sieve to obtain the sea cucumber and abalone oligopeptide powder.
In the above embodiment, the preparation method of the sea cucumber oligopeptide powder comprises the following steps:
collecting sea cucumber cooking liquor, soaking and foaming water, filtering out sea cucumber tissue fragments, silt and other particulate substances in the water by using a cartridge filter, concentrating and desalting filtrate by using an organic membrane with the aperture of 1-2-ten-thousand Da to obtain inorganic salt concentrated solution, separating silt from particulate solids by using a cyclone separator, and subjecting the sea cucumber fragments to enzymolysis;
mixing the sea cucumber tissue fragments with the concentrated solution, adjusting the solid content of the feed liquid to 10-20%, adjusting the temperature of the feed liquid to 40-55 ℃, adjusting the pH of the feed liquid to 2.5-9.0, and adding special compound protease, wherein the proportion of the compound protease is 2709 alkaline protease, 20 ten thousand mu/g, and 10-40%; 30% -60% of As1398 neutral protease with the concentration of 10 ten thousand mu/g; 10-40% of trypsin, 1.0-10% of flavor enzyme and 1.0-5.0% of total protein, stirring for enzymolysis for 3-6h, adjusting the pH of the enzymolysis solution to 5.0-7.0, heating to 70-75 ℃ for enzyme deactivation, removing granular impurities and lipids by using a tubular centrifuge, cooling the filtrate to 10-30 ℃ through a heat exchanger, separating through an organic membrane with the molecular weight cutoff of 5000-20000 Da, and taking the permeate as oligopeptide;
heating the permeate to 50-80 ℃, adding beta-cyclodextrin, wherein the total protein amount of the beta-cyclodextrin is 1-10%, stirring for 20-60 min, passing the fishy smell-removed liquid through a granular activated carbon column for further fishy smell removal, smell removal and decoloration, passing the column-passed liquid through a 50-100 nm ceramic membrane or cotton cake filter for decarburizing to obtain a fine filtrate, further concentrating the fine filtrate through an organic membrane with the pore diameter of 50Da-100Da until the solid content is 17-18%, then carrying out reduced pressure evaporation concentration until the solid content is more than or equal to 40%, and carrying out spray drying to obtain the sea cucumber oligopeptide powder.
In the above embodiment, the preparation method of the abalone oligopeptide powder comprises the following steps:
collecting abalone cooking liquor and soaking water, filtering out sea cucumber tissue fragments, silt and other particulate matters in the water by using a cartridge filter, concentrating and desalting filtrate by using an organic membrane with the aperture of 1-2-ten-thousand Da to obtain inorganic salt concentrated solution, separating silt from particulate solid matters by using a cyclone separator, and subjecting the abalone fragments to enzymolysis;
mixing the abalone tissue fragments with a concentrated solution, adjusting the solid content of the feed liquid to 10-20%, adjusting the temperature of the feed liquid to 40-55 ℃, adjusting the pH of the feed liquid to 2.5-9.0, and adding special compound protease, wherein the proportion of the compound protease is 2709 alkaline protease, 20 ten thousand mu/g, 10-40%; 30% -60% of As1398 neutral protease with the concentration of 10 ten thousand mu/g; 10-40% of trypsin, 1.0-10% of flavor enzyme and 1.0-5.0% of total protein, stirring for enzymolysis for 3-6h, adjusting the pH of the enzymolysis solution to 5.0-7.0, heating to 70-75 ℃ for enzyme deactivation, removing granular impurities and lipids by using a tubular centrifuge, cooling the filtrate to 10-30 ℃ through a heat exchanger, separating through an organic membrane with the molecular weight cutoff of 5000-20000 Da, and taking the permeate as oligopeptide;
heating the permeate to 50-80 ℃, adding beta-cyclodextrin, wherein the total protein amount of the beta-cyclodextrin is 1-10%, stirring for 20-60 min, passing the fishy smell-removed liquid through a granular activated carbon column for further fishy smell removal, smell removal and decoloration, passing the column-passed liquid through a 50-100 nm ceramic membrane or cotton cake filter for decarburizing to obtain a fine filtrate, further concentrating the fine filtrate through an organic membrane with the pore diameter of 50Da-100Da until the solid content is 17-18%, then carrying out reduced pressure evaporation concentration until the solid content is more than or equal to 40%, and carrying out spray drying to obtain the abalone oligopeptide powder.
In the above embodiment, the preparation method of the cod collagen peptide powder comprises the following steps:
selecting cod skin as a raw material, washing the cod skin with clear water, adding a mixed solution of calcium hydroxide and sodium hydroxide, soaking for 8-14 hours, wherein the concentration of the mixed solution of the calcium hydroxide and the sodium hydroxide is 0.2-0.5%, and the weight ratio of the calcium hydroxide to the sodium hydroxide is 1: 2-4, then repeatedly washing with clear water to make the pH value of the mixture neutral;
adding diluted acid water solution with concentration of 0.3-0.6%, soaking for 1-3 hr, and repeatedly washing with clear water to make pH value neutral;
crushing fish skin, stirring and extracting in hot water of 70-80 ℃ for 8-10 hours, and filtering to remove insoluble substances to obtain collagen extracting solution;
adjusting pH of the collagen extract to neutral, adding enzyme for enzymolysis for 3-5 hr under stirring, and allowing enzymolysis temperature to 40-50 deg.C;
heating to 80 ℃, inactivating enzyme of the enzymolysis liquid for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, wherein the air inlet temperature is 180 ℃, the air outlet temperature is 80 ℃, and obtaining cod collagen peptide powder;
vacuum packaging and low-temperature preservation.
In the above embodiment, the preparation method of the oyster oligopeptide powder comprises the following steps:
preparing oyster powder, namely cleaning, draining, pulping and drying fresh oysters to obtain powder;
mixing the slurry, namely mixing the oyster powder and the purified water in a ratio of 1: 2;
performing enzymolysis, namely performing enzymolysis by using neutral protease, wherein the enzyme adding amount is 1 percent of the protein content of a substrate;
centrifuging, adding 30% liquid alkali to adjust pH to 8.0, centrifuging at 50 deg.C for 4 hr, and collecting supernatant;
membrane filtration, namely removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight less than 5ku, and concentrating by using a nanofiltration membrane until the solid content reaches 10%;
heating to 80 ℃, inactivating enzyme of the enzymolysis liquid for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, wherein the air inlet temperature is 180 ℃, the air outlet temperature is 80 ℃, and obtaining the marine oyster oligopeptide powder;
vacuum packaging and low-temperature preservation.
In the above embodiment, the preparation method of the marine mussel oligopeptide powder comprises the following steps:
preparing marine mussel powder, cleaning fresh marine mussels, draining, pulping, and drying to obtain powder;
mixing pulp, namely mixing the marine mussel powder and the purified water in a ratio of 1: 2;
performing enzymolysis, namely performing enzymolysis by using neutral protease, wherein the enzyme adding amount is 1 percent of the protein content of a substrate;
centrifuging, adding 30% liquid alkali to adjust pH to 8.0, centrifuging at 50 deg.C for 4 hr, and collecting supernatant;
membrane filtration, namely removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight less than 5ku, and concentrating by using a nanofiltration membrane until the solid content reaches 10%;
heating to 80 ℃, inactivating enzyme for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, and obtaining marine mussel oligopeptide powder, wherein the air inlet temperature is 180 ℃, and the air outlet temperature is 80 ℃;
vacuum packaging and low-temperature preservation.
In the above embodiment, the preparation method of the tussah pupa oligopeptide powder comprises the following steps:
preparing tussah pupa powder, namely cleaning, draining, pulping and drying fresh tussah pupas into powder;
mixing pulp, namely mixing the tussah pupa powder and the purified water in a ratio of 1: 2;
performing enzymolysis, namely performing enzymolysis by using neutral protease, wherein the enzyme adding amount is 1 percent of the protein content of a substrate;
centrifuging, adding 30% liquid alkali to adjust pH to 8.0, centrifuging at 50 deg.C for 4 hr, and collecting supernatant;
membrane filtration, namely removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight less than 5ku, and concentrating by using a nanofiltration membrane until the solid content reaches 10%;
heating to 80 ℃, inactivating enzyme for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, and obtaining tussah pupa oligopeptide powder, wherein the air inlet temperature is 180 ℃, and the air outlet temperature is 80 ℃;
vacuum packaging and low-temperature preservation.
Claims (8)
1. Sea cucumber, abalone oligopeptide powder, cod collagen peptide powder, oyster oligopeptide powder, sea mussel oligopeptide powder, tussah pupa oligopeptide powder and crude polysaccharide; wherein the total amount of the sea cucumber oligopeptide powder, the abalone oligopeptide powder, the cod collagen peptide powder, the oyster oligopeptide powder, the sea mussel oligopeptide powder and the tussah pupa oligopeptide powder accounts for not less than 90 percent of the total amount of the sea cucumber oligopeptide powder, the abalone oligopeptide powder, the cod collagen peptide powder, the oyster oligopeptide powder, the sea mussel oligopeptide powder, the tussah pupa oligopeptide powder and the crude polysaccharide.
2. A preparation method of sea cucumber and abalone oligopeptide powder is characterized by comprising the following steps:
s1, preparing raw materials, wherein the raw materials comprise sea cucumber oligopeptide powder, abalone oligopeptide powder, cod collagen peptide powder, oyster oligopeptide powder, marine mussel oligopeptide powder, tussah pupa oligopeptide powder and crude polysaccharide;
s2, weighing raw materials, and feeding and mixing the raw materials through a three-dimensional mixer for 60 minutes to obtain a mixture;
s3, filtering the mixture by using a 80-mesh sieve to obtain the sea cucumber and abalone oligopeptide powder.
3. The method for preparing sea cucumber-abalone oligopeptide powder according to claim 2, wherein the method for preparing sea cucumber-abalone oligopeptide powder comprises the following steps:
collecting sea cucumber cooking liquor, soaking and foaming water, filtering out sea cucumber tissue fragments, silt and other particulate substances in the water by using a cartridge filter, concentrating and desalting filtrate by using an organic membrane with the aperture of 1-2-ten-thousand Da to obtain inorganic salt concentrated solution, separating silt from particulate solids by using a cyclone separator, and subjecting the sea cucumber fragments to enzymolysis;
mixing the sea cucumber tissue fragments with the concentrated solution, adjusting the solid content of the feed liquid to 10-20%, adjusting the temperature of the feed liquid to 40-55 ℃, adjusting the pH of the feed liquid to 2.5-9.0, and adding special compound protease, wherein the proportion of the compound protease is 2709 alkaline protease, 20 ten thousand mu/g, and 10-40%; 30% -60% of As1398 neutral protease with the concentration of 10 ten thousand mu/g; 10-40% of trypsin, 1.0-10% of flavor enzyme and 1.0-5.0% of total protein, stirring for enzymolysis for 3-6h, adjusting the pH of the enzymolysis solution to 5.0-7.0, heating to 70-75 ℃ for enzyme deactivation, removing granular impurities and lipids by using a tubular centrifuge, cooling the filtrate to 10-30 ℃ through a heat exchanger, separating through an organic membrane with the molecular weight cutoff of 5000-20000 Da, and taking the permeate as oligopeptide;
heating the permeate to 50-80 ℃, adding beta-cyclodextrin, wherein the total protein amount of the beta-cyclodextrin is 1-10%, stirring for 20-60 min, passing the fishy smell-removed liquid through a granular activated carbon column for further fishy smell removal, smell removal and decoloration, passing the column-passed liquid through a 50-100 nm ceramic membrane or cotton cake filter for decarburizing to obtain a fine filtrate, further concentrating the fine filtrate through an organic membrane with the pore diameter of 50Da-100Da until the solid content is 17-18%, then carrying out reduced pressure evaporation concentration until the solid content is more than or equal to 40%, and carrying out spray drying to obtain the sea cucumber oligopeptide powder.
4. The method for preparing sea cucumber and abalone oligopeptide powder according to claim 2, wherein the method for preparing sea cucumber and abalone oligopeptide powder comprises the following steps:
collecting abalone cooking liquor and soaking water, filtering out sea cucumber tissue fragments, silt and other particulate matters in the water by using a cartridge filter, concentrating and desalting filtrate by using an organic membrane with the aperture of 1-2-ten-thousand Da to obtain inorganic salt concentrated solution, separating silt from particulate solid matters by using a cyclone separator, and subjecting the abalone fragments to enzymolysis;
mixing the abalone tissue fragments with a concentrated solution, adjusting the solid content of the feed liquid to 10-20%, adjusting the temperature of the feed liquid to 40-55 ℃, adjusting the pH of the feed liquid to 2.5-9.0, and adding special compound protease, wherein the proportion of the compound protease is 2709 alkaline protease, 20 ten thousand mu/g, 10-40%; 30% -60% of As1398 neutral protease with the concentration of 10 ten thousand mu/g; 10-40% of trypsin, 1.0-10% of flavor enzyme and 1.0-5.0% of total protein, stirring for enzymolysis for 3-6h, adjusting the pH of the enzymolysis solution to 5.0-7.0, heating to 70-75 ℃ for enzyme deactivation, removing granular impurities and lipids by using a tubular centrifuge, cooling the filtrate to 10-30 ℃ through a heat exchanger, separating through an organic membrane with the molecular weight cutoff of 5000-20000 Da, and taking the permeate as oligopeptide;
heating the permeate to 50-80 ℃, adding beta-cyclodextrin, wherein the total protein amount of the beta-cyclodextrin is 1-10%, stirring for 20-60 min, passing the fishy smell-removed liquid through a granular activated carbon column for further fishy smell removal, smell removal and decoloration, passing the column-passed liquid through a 50-100 nm ceramic membrane or cotton cake filter for decarburizing to obtain a fine filtrate, further concentrating the fine filtrate through an organic membrane with the pore diameter of 50Da-100Da until the solid content is 17-18%, then carrying out reduced pressure evaporation concentration until the solid content is more than or equal to 40%, and carrying out spray drying to obtain the abalone oligopeptide powder.
5. The method for preparing sea cucumber abalone oligopeptide powder according to claim 2, wherein the method for preparing the cod collagen peptide powder comprises the following steps:
selecting cod skin as a raw material, washing the cod skin with clear water, adding a mixed solution of calcium hydroxide and sodium hydroxide, soaking for 8-14 hours, wherein the concentration of the mixed solution of the calcium hydroxide and the sodium hydroxide is 0.2-0.5%, and the weight ratio of the calcium hydroxide to the sodium hydroxide is 1: 2-4, then repeatedly washing with clear water to make the pH value of the mixture neutral;
adding diluted acid water solution with concentration of 0.3-0.6%, soaking for 1-3 hr, and repeatedly washing with clear water to make pH value neutral;
crushing fish skin, stirring and extracting in hot water of 70-80 ℃ for 8-10 hours, and filtering to remove insoluble substances to obtain collagen extracting solution;
adjusting pH of the collagen extract to neutral, adding enzyme for enzymolysis for 3-5 hr under stirring, and allowing enzymolysis temperature to 40-50 deg.C;
heating to 80 ℃, inactivating enzyme of the enzymolysis liquid for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, wherein the air inlet temperature is 180 ℃, the air outlet temperature is 80 ℃, and obtaining cod collagen peptide powder;
vacuum packaging and low-temperature preservation.
6. The method for preparing sea cucumber and abalone oligopeptide powder according to claim 2, wherein the method for preparing oyster oligopeptide powder comprises the following steps:
preparing oyster powder, namely cleaning, draining, pulping and drying fresh oysters to obtain powder;
mixing the slurry, namely mixing the oyster powder and the purified water in a ratio of 1: 2;
performing enzymolysis, namely performing enzymolysis by using neutral protease, wherein the enzyme adding amount is 1 percent of the protein content of a substrate;
centrifuging, adding 30% liquid alkali to adjust pH to 8.0, centrifuging at 50 deg.C for 4 hr, and collecting supernatant;
membrane filtration, namely removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight less than 5ku, and concentrating by using a nanofiltration membrane until the solid content reaches 10%;
heating to 80 ℃, inactivating enzyme of the enzymolysis liquid for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, wherein the air inlet temperature is 180 ℃, the air outlet temperature is 80 ℃, and obtaining the marine oyster oligopeptide powder;
vacuum packaging and low-temperature preservation.
7. The method for preparing sea cucumber and abalone oligopeptide powder according to claim 2, wherein the method for preparing sea mussel oligopeptide powder comprises:
preparing marine mussel powder, cleaning fresh marine mussels, draining, pulping, and drying to obtain powder;
mixing pulp, namely mixing the marine mussel powder and the purified water in a ratio of 1: 2;
performing enzymolysis, namely performing enzymolysis by using neutral protease, wherein the enzyme adding amount is 1 percent of the protein content of a substrate;
centrifuging, adding 30% liquid alkali to adjust pH to 8.0, centrifuging at 50 deg.C for 4 hr, and collecting supernatant;
membrane filtration, namely removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight less than 5ku, and concentrating by using a nanofiltration membrane until the solid content reaches 10%;
heating to 80 ℃, inactivating enzyme for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, and obtaining marine mussel oligopeptide powder, wherein the air inlet temperature is 180 ℃, and the air outlet temperature is 80 ℃;
vacuum packaging and low-temperature preservation.
8. The method for preparing sea cucumber and abalone oligopeptide powder according to claim 2, wherein the method for preparing tussah pupa oligopeptide powder comprises the following steps:
preparing tussah pupa powder, namely cleaning, draining, pulping and drying fresh tussah pupas into powder;
mixing pulp, namely mixing the tussah pupa powder and the purified water in a ratio of 1: 2;
performing enzymolysis, namely performing enzymolysis by using neutral protease, wherein the enzyme adding amount is 1 percent of the protein content of a substrate;
centrifuging, adding 30% liquid alkali to adjust pH to 8.0, centrifuging at 50 deg.C for 4 hr, and collecting supernatant;
membrane filtration, namely removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight less than 5ku, and concentrating by using a nanofiltration membrane until the solid content reaches 10%;
heating to 80 ℃, inactivating enzyme for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, and obtaining tussah pupa oligopeptide powder, wherein the air inlet temperature is 180 ℃, and the air outlet temperature is 80 ℃;
vacuum packaging and low-temperature preservation.
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| CN112914662A (en) * | 2021-03-01 | 2021-06-08 | 卢秀芳 | External pressure blocking hemostat for femoral artery |
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| CN107385001A (en) * | 2017-08-10 | 2017-11-24 | 烟台参福元海洋科技有限公司 | Process for extracting sea cucumber oligopeptide and sea cucumber polysaccharide from sea cucumber deep-processing byproducts |
| CN107828840A (en) * | 2017-11-02 | 2018-03-23 | 林峰 | Silkworm chrysalis active peptide, composition and preparation method |
| CN110720636A (en) * | 2019-11-18 | 2020-01-24 | 大连非得生物产业有限公司 | Yak bone renaturation oligopeptide powder and preparation method thereof |
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| CN101407834A (en) * | 2008-11-05 | 2009-04-15 | 泰祥集团技术开发有限公司 | Method for preparing collagen peptide by using cod skin |
| CN107385001A (en) * | 2017-08-10 | 2017-11-24 | 烟台参福元海洋科技有限公司 | Process for extracting sea cucumber oligopeptide and sea cucumber polysaccharide from sea cucumber deep-processing byproducts |
| CN107828840A (en) * | 2017-11-02 | 2018-03-23 | 林峰 | Silkworm chrysalis active peptide, composition and preparation method |
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