CN110720636A - Yak bone renaturation oligopeptide powder and preparation method thereof - Google Patents
Yak bone renaturation oligopeptide powder and preparation method thereof Download PDFInfo
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- CN110720636A CN110720636A CN201911129818.2A CN201911129818A CN110720636A CN 110720636 A CN110720636 A CN 110720636A CN 201911129818 A CN201911129818 A CN 201911129818A CN 110720636 A CN110720636 A CN 110720636A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
- A23L33/145—Extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a yak bone double-core oligopeptide powder and a preparation method thereof, wherein the yak bone double-core oligopeptide powder comprises the following components in percentage by weight: the yak bone peptide collagen peptide powder is prepared from 40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract and 5% of lutein crystal, can promote bone formation, enhances bone collagen structure under low calcium level, thereby improving the bone strength and achieving the effect of preventing osteoporosis, the oyster is the best food for supplementing calcium, the oyster contains rich phosphorus, and the calcium needs the help of the phosphorus when being absorbed by the body, therefore, the cod collagen peptide is beneficial to the absorption of calcium, the cod collagen peptide can repair the broken elastic fibers of the skin, enhance the tension and elasticity of the skin and completely remove striae gravidarum, the yeast extract contains rich B vitamins, the vitamin B2 can promote the development and the regeneration of cells and promote the normal growth of skin, nails and hair, the lutein can effectively protect the vision, and the lutein has strong antioxidation effect.
Description
Technical Field
The invention relates to the technical field of health products, in particular to yak bone double-core oligopeptide powder and a preparation method thereof.
Background
Modern society develops rapidly, and food is unreasonable, lack of motion, work and rest is irregular, sleep is not enough, mental stress is big, long-term bad mood etc. leads to sub-health, accelerates the health ageing, especially human skeleton and joint, and along with the increase of age, the loss of collagen, skeleton can become more fragile, and the skin also can become shriveled, the deposit of melanin etc. and along with the improvement of quality of life, hyperlipemia also becomes general health problem. With the increasing health awareness of people, there is a growing trend towards non-drug therapies.
The yak bone peptide has high nutritional characteristics, can be used as a metabolism agent, promotes organism collagen biosynthesis, promotes skeleton formation by eating the yak bone peptide, and achieves the effect of preventing osteoporosis. The oyster peptide has the obvious effect of inhibiting platelet aggregation and can reduce the blood fat of patients with hyperlipidemia and the content of TXA2 in blood; it contains rich phosphorus, and is favorable for calcium absorption because calcium is absorbed by the body with the help of phosphorus; the oyster also contains vitamin B12, which is a lack of general food, vitamin B12 contains diamond element which is an indispensable substance for preventing pernicious anemia, and the protein contained in oyster contains a plurality of excellent amino acids which have detoxifying effect and can remove toxic substances in vivo. The cod collagen peptide can rebuild the elasticity of skin, support shriveled skin cells, greatly help postpartum striae gravidarum, supplement collagen in the dermal layer of the skin, effectively prevent the deposition of melanin and lighten color spots. However, health care products integrating the above components do not exist in the market at present, and the existing health care products usually only contain one of the components, so that the health care products have single effect.
Disclosure of Invention
The invention provides yak bone renaturation oligopeptide powder and a preparation method thereof, and aims to solve the problem that the existing health-care product only contains one component of yak bone peptide collagen peptide powder, marine oyster peptide powder and cod protein peptide powder, so that the health-care product has a single effect.
In a first aspect, the invention provides yak bone double-core oligopeptide powder, which comprises the following components in percentage by weight:
40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract, 5% of lutein crystal and 10% of impurities.
In a second aspect, the invention provides a preparation method of yak bone double-core oligopeptide powder, which comprises the following steps:
preparing raw materials, wherein the raw materials comprise yak bone peptide collagen peptide powder, marine oyster peptide powder, cod protein peptide powder, yeast extract and lutein crystals;
weighing the following raw materials in percentage by weight: 40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract and 5% of lutein crystal;
feeding and mixing the raw materials through a three-dimensional mixer for a preset time to obtain a mixture;
and filtering the mixture by using a screen with a preset aperture to obtain the yak bone double-core oligopeptide powder.
Optionally, the preparation method of the bovine bone peptide collagen peptide powder comprises the following steps:
pretreating raw materials, washing fresh or frozen yak bone with high-pressure cold water to remove foreign matters, putting the washed yak bone into a mincing machine, crushing to 20-30mm, and placing in a clean container;
extracting gelatin, accurately metering crushed yak bones, putting into a cooking pot, adding tap water 1.2 times of the weight of the yak bones through a pipeline flowmeter, sealing a feeding port, and cooking with hot water to obtain a mixed solution containing oil and gelatin;
separating collagen extracting solution, after extraction, opening a liquid discharge valve at the bottom of the cooking tank and a feeding valve of a liquid storage tank, observing a sight glass, closing the feeding valve of the liquid storage tank in time when the mixed solution flows to a water-oil interface, opening a valve of the oil storage tank, and discharging oil into the oil storage tank, so that the oil and the gelatin are completely separated;
performing compound enzymolysis, namely performing enzymolysis in a liquid storage tank by adopting a compound enzyme enzymolysis method, so that the hydrolysis degree of protein can be improved, and more hydrolysate with small molecular weight can be obtained; the enzymolysis temperature is 48-52 ℃, and the time is 4-6 hours;
inactivating enzyme, sterilizing and separating peptide liquid, boiling enzymolysis liquid for 20 minutes after enzymolysis is finished, inactivating enzyme, sterilizing soup liquid, standing for 30 minutes, layering the hydrolysis liquid, wherein the upper layer is pulp of non-enzymolysis macromolecular denatured protein and inactivated enzyme, the lower layer is clear and transparent peptide liquid, and after vacuum filtration, the clarified filtrate enters a filtrate storage tank to obtain collagen peptidase hydrolysate;
decoloring and deodorizing, wherein the prepared collagen peptidase hydrolysate is light yellow, and the powdery active carbon is used as a decoloring and deodorizing agent to decolor and deodorize the enzymolysis solution;
concentrating, controlling the temperature of the collagen peptidase hydrolyzed solution at 80-82 deg.C, concentrating the collagen peptidase hydrolyzed solution in a concentrator for no more than 30 minutes under the vacuum degree of 0.06-0.08Mpa until the baume degree is 20-22 Be, and storing the concentrated solution in a concentrated solution storage tank for temporary storage;
spray drying, wherein the material temperature is 45-50 ℃, the Baume degree is 20-22 Bee, the air inlet temperature of a spray tower is adjusted within the range of 150-160 ℃, the air outlet temperature is 80-90 ℃, and the powder is dried to obtain the yak bone peptide collagen peptide powder.
Optionally, the preparation method of the marine oyster peptide powder comprises the following steps:
preparing oyster powder, namely cleaning, draining, pulping and drying fresh oysters to obtain powder;
mixing the slurry, namely mixing the oyster powder and the purified water in a ratio of 1: 2;
performing enzymolysis, namely performing enzymolysis by using neutral protease, wherein the enzyme adding amount is 1 percent of the protein content of a substrate;
centrifuging, adding 30% liquid alkali to adjust pH to 8.0, centrifuging at 50 deg.C for 4 hr, and collecting supernatant;
membrane filtration, namely removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight less than 5ku, and concentrating by using a nanofiltration membrane until the solid content reaches 10%;
heating to 80 ℃, inactivating enzyme for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, and obtaining marine oyster peptide powder, wherein the air inlet temperature is 180 ℃, and the air outlet temperature is 80 ℃;
vacuum packaging and low-temperature preservation.
Optionally, the preparation method of the cod collagen peptide comprises the following steps:
selecting cod skin as a raw material, washing the cod skin with clear water, adding a mixed solution of calcium hydroxide and sodium hydroxide, soaking for 8-14 hours, wherein the concentration of the mixed solution of calcium hydroxide and sodium hydroxide is 0.2-0.5%, the weight ratio of the calcium hydroxide to the sodium hydroxide is 1:2-4, and then repeatedly washing with clear water to enable the pH value of the cod skin to be neutral;
adding diluted acid water solution with concentration of 0.3-0.6%, soaking for 1-3 hr, and repeatedly washing with clear water to make pH value neutral;
crushing fish skin, stirring and extracting in hot water of 70-80 ℃ for 8-10 hours, and filtering to remove insoluble substances to obtain collagen extracting solution;
adjusting pH of the collagen extract to neutral, adding enzyme for enzymolysis for 3-5 hr under stirring, and allowing enzymolysis temperature to 40-50 deg.C;
heating to 80 ℃, inactivating enzyme of the enzymolysis liquid for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, wherein the air inlet temperature is 180 ℃, the air outlet temperature is 80 ℃, and obtaining the cod collagen peptide;
vacuum packaging and low-temperature preservation.
Optionally, the preparation method of the yeast extract comprises:
sieving, washing, separating, sieving with 200 mesh sieve, removing impurities in yeast, washing off some impurities such as ash and emulsified substances to obtain yeast solution, and deodorizing the yeast with weak alkaline solution of five per thousand;
centrifuging at the rotating speed of 3500-;
preparing suspension, namely preparing the yeast paste obtained in the last step into 10-15% of yeast suspension;
autolysis, wherein the autolysis time is 24-28h under the conditions that the pH value is 5.5-6.5 and the temperature is 40-55 ℃;
heating to inactivate enzyme, and heating to 90-95 deg.C for 15-20min after autolysis to inactivate enzymes in yeast cells;
performing centrifugal separation, cooling the enzyme-inactivated semi-finished product, and centrifuging for 30min at the rotating speed of 3500-;
concentrating and drying, concentrating the supernatant obtained by centrifugation at 60 deg.C under reduced pressure to dry matter concentration of 40%, and spray drying at air inlet temperature of 165 deg.C and air outlet temperature of 90 deg.C to obtain powdered yeast extract.
Optionally, the preparation method of the lutein crystal comprises:
preparing marigold pollen, namely taking petals of fresh marigold flowers, performing light-resistant fermentation treatment, adding liquid nitrogen, and grinding into powder of 40-60um to obtain marigold pollen;
preparing lutein, namely 50g of marigold pollen, 500ml of tetrahydrofuran, KOH12.5g of ethanol and 62ml of water, stirring and mixing for 2 hours at room temperature under the conditions of shading and sealing, wherein the pH value of the mixed solution is about l 2; filtering, performing rotary evaporation on the filtrate to remove the solvent, washing the residue with water until the pH value of the washing liquid becomes neutral, and centrifuging to obtain a lutein crude product with the purity of 70.8%;
dissolving the lutein crude product in a small amount of tetrahydrofuran, adding water until the solution is turbid, stirring at room temperature under a light-shielding and sealing condition to recrystallize the compound, then centrifugally separating out a solid, washing the solid with water to remove the tetrahydrofuran, and finally performing vacuum drying at 0 ℃ to obtain the lutein crystal.
The invention has the following beneficial effects:
the invention provides yak bone double-core oligopeptide powder and a preparation method thereof, wherein the yak bone double-core oligopeptide powder comprises the following components in percentage by weight: the bone collagen peptide powder is prepared from 40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract and 5% of lutein crystal, wherein the yak bone peptide collagen peptide powder can promote the formation of bones and strengthen the bone collagen structure under the low calcium level, so that the bone strength is improved, the effect of preventing osteoporosis is achieved, the bone collagen peptide powder has good prevention and treatment effects on collagen such as arthrosis and the like, and the aging and the hypofunction of biological tissues caused by the aging are improved; oyster is the best food for supplementing calcium, contains abundant phosphorus, is beneficial to calcium absorption because the calcium is absorbed by the body by the aid of phosphorus, and also contains vitamin B12 which is lacked by common foods; the cod collagen peptide can repair the broken elastic fibers of the skin, enhance the tension and elasticity of the skin, thoroughly remove striae gravidarum, keep moisture required by the tender skin, effectively prevent the deposition of melanin, fade color spots, remove dark yellow and have the anti-aging effect; the yeast extract contains rich B vitamins, vitamin B2 can promote development and cell regeneration, promote normal growth of skin, nail and hair, vitamin B12 is an indispensable substance for promoting growth, keeping nerve tissue healthy and forming normal red blood cells (erythrocytes), can maintain normal functions of the nervous system and promote synthesis of myelin of nerve myelin; the lutein can effectively protect eyesight, has strong antioxidant effect, can inhibit activity of active oxygen free radicals, prevent damage of the active oxygen free radicals to normal cells, enhance the immunity of an organism, and has a delaying effect on early arteriosclerosis.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
Fig. 1 is a flow chart of a preparation method of yak bone double-core oligopeptide powder provided by the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the specific embodiments of the present invention and the accompanying drawings. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The technical solutions provided by the embodiments of the present invention are described in detail below with reference to the accompanying drawings.
Firstly, the invention provides yak bone double-core oligopeptide powder which comprises the following components in percentage by weight:
40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract, 5% of lutein crystal and 10% of impurities.
Secondly, as shown in figure 1, the invention provides a preparation method of yak bone double-core oligopeptide powder, which comprises the following steps:
the preparation method comprises the following steps of preparing raw materials, wherein the raw materials comprise yak bone peptide collagen peptide powder, marine oyster peptide powder, cod protein peptide powder, a yeast extract and lutein crystals.
Step two, weighing the following raw materials in percentage by weight: 40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract and 5% of lutein crystal.
And step three, feeding and mixing the raw materials through a three-dimensional mixer, and mixing for a preset time to obtain a mixture.
And step four, filtering the mixture by using a screen with a preset aperture to obtain the yak bone double-core oligopeptide powder.
In this embodiment, the preparation process of the yak bone peptide collagen peptide powder is as follows:
(1) pretreatment of raw materials: washing fresh or frozen yak bone with high pressure cold water to remove foreign matter, putting the washed yak bone into a mincing machine, crushing to 20-30mm, and placing in a clean container;
(2) gelatin extraction: accurately metering crushed yak bones, putting the yak bones into a cooking pot, adding tap water with the weight 1.2 times that of the yak bones through a pipeline type flowmeter, sealing a feeding port, and cooking the yak bones into mixed liquid containing grease and gelatin by hot water;
(3) separating collagen extracting solution: after extraction is finished, a liquid discharge valve at the bottom of the cooking tank and a feeding valve of the liquid storage tank are opened, a viewing mirror is observed, when the mixed liquid flows to a water-oil interface, the feeding valve of the liquid storage tank is closed in time, a valve of the oil storage tank is opened, and the oil is discharged into the oil storage tank, so that the oil and the gelatin are completely separated;
(4) compound enzymolysis: the enzymolysis is carried out in the liquid storage tank by adopting a method of compound enzyme enzymolysis, so that the hydrolysis degree of protein can be improved, and more hydrolysis products with small molecular weight can be obtained. The enzymolysis temperature is 48-52 ℃, and the time is 4-6 hours;
(5) enzyme deactivation, sterilization and peptide liquid separation: after enzymolysis is finished, boiling the enzymolysis liquid for 20 minutes to inactivate enzyme, sterilizing the soup, standing for 30 minutes, layering the hydrolysis liquid, wherein the upper layer is pulp of non-enzymolysis macromolecular denatured protein and inactivated enzyme, the lower layer is clear and transparent peptide liquid, and after vacuum filtration, the clear filtrate enters a filtrate storage tank to obtain collagen peptidase hydrolysate;
(6) decoloring and deodorizing: the prepared collagen peptidase hydrolyzed solution is light yellow, and powdered activated carbon is used as a decoloring and deodorizing agent to decolor and deodorize the enzymolysis solution;
(7) concentration: controlling the temperature of the collagen peptidase hydrolyzed liquid at 80-82 ℃, concentrating the material fed into a concentrator in each batch for no more than 30 minutes under the vacuum degree of 0.06-0.08Mpa until the baume degree is 20-22 Be, and temporarily storing the concentrated solution in a concentrated solution storage tank;
(8) spray drying: the temperature of the material is 45-50 ℃, the Baume degree is 20-22 Bee, the air inlet temperature of the spray tower is adjusted within the range of 150-160 ℃, the air outlet temperature is 80-90 ℃, and the material is dried into powder, namely the yak bone peptide collagen peptide powder.
In this embodiment, the preparation process of the marine oyster peptide powder is as follows:
(1) preparing oyster powder: cleaning fresh oysters, draining, pulping, and drying into powder;
(2) size mixing: mixing the oyster powder and purified water in a ratio of 1: 2;
(3) enzymolysis: carrying out enzymolysis by neutral protease, wherein the enzyme adding amount is 1% of the protein content of the substrate;
(4) centrifugal separation: after enzymolysis, adding 30% liquid alkali to adjust the pH of the enzymolysis liquid to 8.0, centrifuging for 4 hours at 50 ℃, and taking the supernatant;
(5) membrane filtration: removing impurities with ceramic membrane, filtering with ultrafiltration membrane to obtain polypeptide with molecular weight less than 5ku, and concentrating with nanofiltration membrane until solid content reaches 10%;
(6) heating to 80 ℃, inactivating enzyme for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying (air inlet temperature 180 ℃, air outlet temperature 80 ℃) to obtain marine oyster peptide powder;
(7) vacuum packaging and low-temperature preservation.
In this embodiment, the preparation process of the cod collagen peptide is as follows:
(1) selecting cod skin as a raw material, washing the cod skin with clear water, adding a mixed solution of calcium hydroxide and sodium hydroxide, soaking for 8-14 hours, wherein the concentration of the mixed solution of calcium hydroxide and sodium hydroxide is 0.2-0.5%, the weight ratio of the calcium hydroxide to the sodium hydroxide is 1:2-4, and then repeatedly washing with clear water to enable the pH value of the cod skin to be neutral;
(2) adding diluted acid water solution with concentration of 0.3-0.6%, soaking for 1-3 hr, and repeatedly washing with clear water to make pH value neutral;
(3) crushing fish skin, stirring and extracting in hot water of 70-80 ℃ for 8-10 hours, and filtering to remove insoluble substances to obtain collagen extracting solution;
(4) adjusting pH of the collagen extract to neutral, adding enzyme for enzymolysis for 3-5 hr under stirring, and allowing enzymolysis temperature to 40-50 deg.C;
(5) heating to 80 ℃, inactivating enzyme for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying (air inlet temperature 180 ℃, air outlet temperature 80 ℃) to obtain the cod collagen peptide.
(6) Vacuum packaging, and storing at low temperature
In this example, the yeast extract was prepared as follows:
(1) sieving, washing, separating, sieving with 200 mesh sieve, removing impurities in yeast, washing off some impurities such as ash and emulsified substances to obtain yeast solution, and deodorizing the yeast with weak alkaline solution of five per thousand;
(2) centrifuging: centrifuging at 3500-;
(3) preparing a suspension: preparing the yeast paste obtained in the last step into 10-15% of yeast suspension;
(4) autolysis: at the pH of 5.5-6.5 and the temperature of 40-55 ℃, the autolysis time is 24-28 h;
(5) heating to inactivate enzyme: after autolysis is finished, heating to 90-95 ℃ for 15-20min to inactivate enzymes in the yeast cells;
(6) centrifugal separation: cooling the enzyme-inactivated semi-finished product, and centrifuging for 30min at the rotating speed of 3500-;
(7) concentrating and drying: centrifuging the obtained autolyzed supernatant, controlling the temperature at 60 ℃, concentrating under reduced pressure to dry matter concentration of 40% (semi-solid state), and then spraying and drying into powder under certain conditions (inlet air temperature of 165 ℃ and outlet air temperature of 90 ℃) to obtain the powdery yeast extract.
In this embodiment, the preparation process of the lutein crystal is as follows:
(1) preparation method of marigold pollen
Fermenting fresh flos Tagetis Erectae petal in dark place, adding liquid nitrogen, and grinding into powder (40-60um) to obtain flos Tagetis Erectae pollen.
(2) Preparation of xanthophylls
50g of marigold pollen, 500ml of tetrahydrofuran (containing 0.01% of BHT antioxidant), 12.5g of KOH12, 62ml of ethanol and 62ml of water are stirred and mixed for 2 hours at room temperature under the conditions of shading and sealing, and the pH value of the mixed solution is about l 2. Filtering, rotary evaporating filtrate to remove solvent, washing residue with water until pH of washing solution is neutral, centrifuging to obtain xanthophyll crude product with purity of 70.8%.
(3) Preparation of crystalline lutein
Dissolving the xanthophyll crude product in a small amount of tetrahydrofuran (containing 0.01% BHT), adding water until the solution is turbid, stirring under the conditions of room temperature, shading and sealing to recrystallize the compound, then centrifugally separating out solid, washing the solid with water to remove the tetrahydrofuran, and finally performing vacuum drying at 0 ℃ to obtain the xanthophyll crystal.
According to the technical scheme, the yak bone double-core oligopeptide powder and the preparation method thereof provided by the embodiment of the invention comprise the following components in percentage by weight: the bone collagen peptide powder is prepared from 40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract and 5% of lutein crystal, wherein the yak bone peptide collagen peptide powder can promote the formation of bones and strengthen the bone collagen structure under the low calcium level, so that the bone strength is improved, the effect of preventing osteoporosis is achieved, the bone collagen peptide powder has good prevention and treatment effects on collagen such as arthrosis and the like, and the aging and the hypofunction of biological tissues caused by the aging are improved; oyster is the best food for supplementing calcium, contains abundant phosphorus, is beneficial to calcium absorption because the calcium is absorbed by the body by the aid of phosphorus, and also contains vitamin B12 which is lacked by common foods; the cod collagen peptide can repair the broken elastic fibers of the skin, enhance the tension and elasticity of the skin, thoroughly remove striae gravidarum, keep moisture required by the tender skin, effectively prevent the deposition of melanin, fade color spots, remove dark yellow and have the anti-aging effect; the yeast extract contains rich B vitamins, vitamin B2 can promote development and cell regeneration, promote normal growth of skin, nail and hair, vitamin B12 is an indispensable substance for promoting growth, keeping nerve tissue healthy and forming normal red blood cells (erythrocytes), can maintain normal functions of the nervous system and promote synthesis of myelin of nerve myelin; the lutein can effectively protect eyesight, has strong antioxidant effect, can inhibit activity of active oxygen free radicals, prevent damage of the active oxygen free radicals to normal cells, enhance the immunity of an organism, and has a delaying effect on early arteriosclerosis.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The yak bone double-core oligopeptide powder is characterized by comprising the following components in percentage by weight:
40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract, 5% of lutein crystal and 10% of impurities.
2. A preparation method of yak bone double-core oligopeptide powder is characterized by comprising the following steps:
preparing raw materials, wherein the raw materials comprise yak bone peptide collagen peptide powder, marine oyster peptide powder, cod protein peptide powder, yeast extract and lutein crystals;
weighing the following raw materials in percentage by weight: 40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract and 5% of lutein crystal;
feeding and mixing the raw materials through a three-dimensional mixer for a preset time to obtain a mixture;
and filtering the mixture by using a screen with a preset aperture to obtain the yak bone double-core oligopeptide powder.
3. The method for preparing yak bone renaturation oligopeptide powder according to claim 2, wherein the method for preparing the yak bone peptide collagen peptide powder comprises the following steps:
pretreating raw materials, washing fresh or frozen yak bone with high-pressure cold water to remove foreign matters, putting the washed yak bone into a mincing machine, crushing to 20-30mm, and placing in a clean container;
extracting gelatin, accurately metering crushed yak bones, putting into a cooking pot, adding tap water 1.2 times of the weight of the yak bones through a pipeline flowmeter, sealing a feeding port, and cooking with hot water to obtain a mixed solution containing oil and gelatin;
separating collagen extracting solution, after extraction, opening a liquid discharge valve at the bottom of the cooking tank and a feeding valve of a liquid storage tank, observing a sight glass, closing the feeding valve of the liquid storage tank in time when the mixed solution flows to a water-oil interface, opening a valve of the oil storage tank, and discharging oil into the oil storage tank, so that the oil and the gelatin are completely separated;
performing compound enzymolysis, namely performing enzymolysis in a liquid storage tank by adopting a compound enzyme enzymolysis method, so that the hydrolysis degree of protein can be improved, and more hydrolysate with small molecular weight can be obtained; the enzymolysis temperature is 48-52 ℃, and the time is 4-6 hours;
inactivating enzyme, sterilizing and separating peptide liquid, boiling enzymolysis liquid for 20 minutes after enzymolysis is finished, inactivating enzyme, sterilizing soup liquid, standing for 30 minutes, layering the hydrolysis liquid, wherein the upper layer is pulp of non-enzymolysis macromolecular denatured protein and inactivated enzyme, the lower layer is clear and transparent peptide liquid, and after vacuum filtration, the clarified filtrate enters a filtrate storage tank to obtain collagen peptidase hydrolysate;
decoloring and deodorizing, wherein the prepared collagen peptidase hydrolysate is light yellow, and the powdery active carbon is used as a decoloring and deodorizing agent to decolor and deodorize the enzymolysis solution;
concentrating, controlling the temperature of the collagen peptidase hydrolyzed solution at 80-82 deg.C, concentrating the collagen peptidase hydrolyzed solution in a concentrator for no more than 30 minutes under the vacuum degree of 0.06-0.08Mpa until the baume degree is 20-22 Be, and storing the concentrated solution in a concentrated solution storage tank for temporary storage;
spray drying, wherein the material temperature is 45-50 ℃, the Baume degree is 20-22 Bee, the air inlet temperature of a spray tower is adjusted within the range of 150-160 ℃, the air outlet temperature is 80-90 ℃, and the powder is dried to obtain the yak bone peptide collagen peptide powder.
4. The method for preparing yak bone renaturation oligopeptide powder according to claim 2, wherein the method for preparing the marine oyster peptide powder comprises the following steps:
preparing oyster powder, namely cleaning, draining, pulping and drying fresh oysters to obtain powder;
mixing the slurry, namely mixing the oyster powder and the purified water in a ratio of 1: 2;
performing enzymolysis, namely performing enzymolysis by using neutral protease, wherein the enzyme adding amount is 1 percent of the protein content of a substrate;
centrifuging, adding 30% liquid alkali to adjust pH to 8.0, centrifuging at 50 deg.C for 4 hr, and collecting supernatant;
membrane filtration, namely removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight less than 5ku, and concentrating by using a nanofiltration membrane until the solid content reaches 10%;
heating to 80 ℃, inactivating enzyme for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, and obtaining marine oyster peptide powder, wherein the air inlet temperature is 180 ℃, and the air outlet temperature is 80 ℃;
vacuum packaging and low-temperature preservation.
5. The method for preparing the yak bone renaturation oligopeptide powder according to the claim 2, wherein the preparation method of the cod collagen peptide comprises the following steps:
selecting cod skin as a raw material, washing the cod skin with clear water, adding a mixed solution of calcium hydroxide and sodium hydroxide, soaking for 8-14 hours, wherein the concentration of the mixed solution of calcium hydroxide and sodium hydroxide is 0.2-0.5%, the weight ratio of the calcium hydroxide to the sodium hydroxide is 1:2-4, and then repeatedly washing with clear water to enable the pH value of the cod skin to be neutral;
adding diluted acid water solution with concentration of 0.3-0.6%, soaking for 1-3 hr, and repeatedly washing with clear water to make pH value neutral;
crushing fish skin, stirring and extracting in hot water of 70-80 ℃ for 8-10 hours, and filtering to remove insoluble substances to obtain collagen extracting solution;
adjusting pH of the collagen extract to neutral, adding enzyme for enzymolysis for 3-5 hr under stirring, and allowing enzymolysis temperature to 40-50 deg.C;
heating to 80 ℃, inactivating enzyme of the enzymolysis liquid for 10 minutes, filtering, concentrating in vacuum to 20% concentration, spray drying, wherein the air inlet temperature is 180 ℃, the air outlet temperature is 80 ℃, and obtaining the cod collagen peptide;
vacuum packaging and low-temperature preservation.
6. The method for preparing yak bone renaturation oligopeptide powder according to claim 2, wherein the method for preparing the yeast extract comprises the following steps:
sieving, washing, separating, sieving with 200 mesh sieve, removing impurities in yeast, washing off some impurities such as ash and emulsified substances to obtain yeast solution, and deodorizing the yeast with weak alkaline solution of five per thousand;
centrifuging at the rotating speed of 3500-;
preparing suspension, namely preparing the yeast paste obtained in the last step into 10-15% of yeast suspension;
autolysis, wherein the autolysis time is 24-28h under the conditions that the pH value is 5.5-6.5 and the temperature is 40-55 ℃;
heating to inactivate enzyme, and heating to 90-95 deg.C for 15-20min after autolysis to inactivate enzymes in yeast cells;
performing centrifugal separation, cooling the enzyme-inactivated semi-finished product, and centrifuging for 30min at the rotating speed of 3500-;
concentrating and drying, concentrating the supernatant obtained by centrifugation at 60 deg.C under reduced pressure to dry matter concentration of 40%, and spray drying at air inlet temperature of 165 deg.C and air outlet temperature of 90 deg.C to obtain powdered yeast extract.
7. The method for preparing yak bone renaturation oligopeptide powder according to claim 2, wherein the method for preparing the lutein crystal comprises the following steps:
preparing marigold pollen, namely taking petals of fresh marigold flowers, performing light-resistant fermentation treatment, adding liquid nitrogen, and grinding into powder of 40-60um to obtain marigold pollen;
preparing lutein, namely 50g of marigold pollen, 500ml of tetrahydrofuran, 12.5g of KOH, 62ml of ethanol and 62ml of water, stirring and mixing for 2 hours at room temperature under the conditions of shading and sealing, wherein the pH value of the mixed solution is about l 2; filtering, performing rotary evaporation on the filtrate to remove the solvent, washing the residue with water until the pH value of the washing liquid becomes neutral, and centrifuging to obtain a lutein crude product with the purity of 70.8%;
dissolving the lutein crude product in a small amount of tetrahydrofuran, adding water until the solution is turbid, stirring at room temperature under a light-shielding and sealing condition to recrystallize the compound, then centrifugally separating out a solid, washing the solid with water to remove the tetrahydrofuran, and finally performing vacuum drying at 0 ℃ to obtain the lutein crystal.
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| CN115736258A (en) * | 2022-12-29 | 2023-03-07 | 大连非得生物产业有限公司 | Yak bone renaturation oligopeptide powder and preparation method thereof |
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