CN103783254A - Preparation method of yak bone collagen peptide - Google Patents
Preparation method of yak bone collagen peptide Download PDFInfo
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- CN103783254A CN103783254A CN201310271584.1A CN201310271584A CN103783254A CN 103783254 A CN103783254 A CN 103783254A CN 201310271584 A CN201310271584 A CN 201310271584A CN 103783254 A CN103783254 A CN 103783254A
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- yak bone
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- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 32
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 26
- 102000008186 Collagen Human genes 0.000 title claims abstract description 20
- 108010035532 Collagen Proteins 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 229920001436 collagen Polymers 0.000 title claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 claims abstract description 30
- 108090000790 Enzymes Proteins 0.000 claims abstract description 30
- 108010010803 Gelatin Proteins 0.000 claims abstract description 19
- 229920000159 gelatin Polymers 0.000 claims abstract description 19
- 239000008273 gelatin Substances 0.000 claims abstract description 19
- 235000019322 gelatine Nutrition 0.000 claims abstract description 19
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000000605 extraction Methods 0.000 claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 108091005804 Peptidases Proteins 0.000 claims abstract description 5
- 239000004365 Protease Substances 0.000 claims abstract description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 5
- 230000009849 deactivation Effects 0.000 claims abstract description 5
- 239000004382 Amylase Substances 0.000 claims abstract description 4
- 102000013142 Amylases Human genes 0.000 claims abstract description 4
- 108010065511 Amylases Proteins 0.000 claims abstract description 4
- 235000019418 amylase Nutrition 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims description 27
- 239000007788 liquid Substances 0.000 claims description 21
- 230000007062 hydrolysis Effects 0.000 claims description 15
- 238000006460 hydrolysis reaction Methods 0.000 claims description 15
- 238000004332 deodorization Methods 0.000 claims description 13
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 13
- 239000004519 grease Substances 0.000 claims description 10
- 239000012141 concentrate Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000002203 pretreatment Methods 0.000 claims description 6
- 235000014347 soups Nutrition 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 5
- 235000019419 proteases Nutrition 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 102000029816 Collagenase Human genes 0.000 claims description 3
- 108060005980 Collagenase Proteins 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 238000005352 clarification Methods 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000010411 cooking Methods 0.000 claims description 3
- 230000007850 degeneration Effects 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 239000000413 hydrolysate Substances 0.000 claims description 3
- 150000002605 large molecules Chemical class 0.000 claims description 3
- 229920002521 macromolecule Polymers 0.000 claims description 3
- -1 pectase Proteins 0.000 claims description 3
- 230000007065 protein hydrolysis Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000002002 slurry Substances 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 238000003828 vacuum filtration Methods 0.000 claims description 3
- 239000002131 composite material Substances 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 4
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 4
- 230000001877 deodorizing effect Effects 0.000 abstract 2
- 238000000926 separation method Methods 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 abstract 1
- 108010059820 Polygalacturonase Proteins 0.000 abstract 1
- 229920002678 cellulose Polymers 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 abstract 1
- 108010093305 exopolygalacturonase Proteins 0.000 abstract 1
- 239000011259 mixed solution Substances 0.000 abstract 1
- 238000012414 sterilization procedure Methods 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 229920002567 Chondroitin Polymers 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000001479 atomic absorption spectroscopy Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940036811 bone meal Drugs 0.000 description 1
- 239000002374 bone meal Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- DLGJWSVWTWEWBJ-HGGSSLSASA-N chondroitin Chemical compound CC(O)=N[C@@H]1[C@H](O)O[C@H](CO)[C@H](O)[C@@H]1OC1[C@H](O)[C@H](O)C=C(C(O)=O)O1 DLGJWSVWTWEWBJ-HGGSSLSASA-N 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229910052745 lead Inorganic materials 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method of yak bone collagen peptide. The method is characterized by comprising the following technological processes: pretreatment of raw materials, gelatin extraction, separation of a collagen extracting solution, composite enzymatic hydrolysis, enzyme deactivation and sterilization, separation of a peptide solution, decoloring and deodorizing, concentrating and drying. The compound enzyme for composite enzymatic hydrolysis is prepared from protease, amylase, pectinase, cellulose and xylanase. The method has the advantages that the gelatin extraction process is simple, oil and gelatin are completely separated by using flow of a mixed solution, the enzymatic hydrolysis temperature is 48-52 DEG C, the time is 4-6 hours, the temperature is reduced in comparison with common enzymatic hydrolysis temperature, the preparation time of the product is shortened, and the method comprises enzyme deactivation and sterilization procedures, and decoloring and deodorizing procedures, so that the product is good in taste, and applicable to preparation of the yak bone collagen peptide.
Description
Technical field
The invention belongs to and derive from mammiferous medicinal preparation, particularly a kind of collagen peptide health-care food that utilizes Yak Bone to make.
Background technology
Qinghai has abundant Yak Bone resource, and exploitation in recent years has bone meal, gelatin, peptone, the health foods such as basic biochemistry raw material and yak marrow powder such as yak chondroitin.Through Atomic Absorption Spectrometry, in Yak Bone, contain K, Na, Ca, Mg, P, Cu, Zn, Fe, Mn, Ni, Pb, 12 kinds of mineral matter elements such as Cd, to the very important function of having grown of skeleton.
Although it is a lot of to utilize Yak Bone to cook health food now, its method for making is generally Yak Bone through thermophilic digestion, pulverizes, and the degreasing of soup juice, grinds, sterilizing, and homogeneous, concentrated, the dry powder process of spraying.Its technical process is failed little protein molecularization, and the nutritional labeling of Yak Bone is failed fully to develop.In recent years the preparation method who has again Yak bone peptide, starts to utilize zymolysis technique, and its preparation technology is for preparing bone soup, then enzymolysis bone soup, and its enzymolysis has multiple with enzyme, and as compound protease, composite flavor enzyme, peptase or trypsase etc., its hydrolysis result is different.
Summary of the invention
The object of the invention is to, Yak Bone is carried out to collagen extractive process and carry out gelatin extraction and complex enzyme hydrolysis, obtain the amino acid whose collagen peptide of little molecule, be beneficial to absorption of human body.
Technical scheme of the present invention is, a kind of preparation method of Yak Bone collagen protein peptides, and its processing step comprises raw material pre-treatment, gelatin extraction, collagen extract, complex enzyme hydrolysis, the enzyme sterilizing of going out, isolated peptides liquid, decoloration deodorization, concentrate drying, concrete steps are:
(1), the pre-treatment of raw material: by the fresh or freezing Yak Bone of high-pressure cold water flushing, to remove foreign matter, then the Yak Bone of cleaning is dropped into pulverizer, crush the size to 20-30mm, be positioned in clean container;
(2), gelatin extraction: will pack cooker into after Yak Bone accurate-metering, add the running water of 1.2 times of Yak Bone weight by duct type flowmeter, sealing dog-house, the mixed liquor that hot cooking becomes to contain grease and gelatin;
(3), collagen extract: after extraction finishes, draining valve at the bottom of unlatching cooker and the material inlet valve of receiver, observe visor, in the time that flowing to water oil content interface, mixed liquor closes in time receiver material inlet valve, open oil tank valve, grease is entered to oil tank, and grease separates completely with gelatin like this;
(4), complex enzyme hydrolysis: adopt the method for complex enzyme zymohydrolysis, carry out enzymolysis at receiver, can improve the degree of hydrolysis of protein, obtain the hydrolysate of how little molecular weight.Hydrolysis temperature is 48-52 ℃, and the time is 4-6 hour;
(5), go out enzyme, sterilizing, isolated peptides liquid: after enzymolysis finishes, enzymolysis liquid is boiled 20 minutes, make enzyme-deactivating, make soup sterilizing simultaneously, then leave standstill 30 minutes, at this moment hydrolyzate layering, upper strata is the slurry of the large molecule degeneration albumen of not enzymolysis and the enzyme of deactivation, lower floor is limpid transparent peptide liquid; Enzymolysis liquid is through vacuum filtration, and the filtrate of clarification enters filtrate tank, is peptide liquid;
(6), decoloration deodorization: the collagen peptidase hydrolyzed liquor making is above light yellow, take powdered active carbon as decoloration deodorization agent, enzymolysis liquid is carried out to decoloration deodorization;
(7), concentrated: temperature of charge is controlled at 80～82 ℃, and under 0.06～0.08Mpa vacuum, every batch of material concentration time of squeezing into inspissator is no more than 30 minutes, is concentrated into Baume degrees 20～22B é, and it is temporary that concentrate enters concentrate hold-up tank;
(8), spraying is dry: 45～50 ℃ of temperature of charge, Baume degrees 20～22B é, spray tower EAT is adjusted in 150～160 ℃ of scopes, leaving air temp, 80～90 ℃ of scopes, is dried to powder.
The preparation method of Yak Bone collagen protein peptides as above, its complex enzyme adopting is made up of protease, amylase, pectase, cellulase, zytase.
Advantage of the present invention is that gelatin extraction process is simple, utilizes flowing of mixed liquor, and grease is separated completely with gelatin; Hydrolysis temperature is 48-52 ℃, and the time is 4-6 hour, and more general hydrolysis temperature is low, has shortened product preparation time; There are the enzyme of going out, sterilization process, have decoloration deodorization operation, make the edible taste of product better.Be applicable to the preparation of Yak Bone collagen protein peptides.
The specific embodiment
A preparation method for Yak Bone collagen protein peptides, its processing step comprises raw material pre-treatment, gelatin extraction, collagen extract, complex enzyme hydrolysis, the enzyme sterilizing of going out, isolated peptides liquid, decoloration deodorization, concentrate drying, concrete steps are:
(1), the pre-treatment of raw material: by the fresh or freezing Yak Bone of high-pressure cold water flushing, to remove foreign matter, then the Yak Bone of cleaning is dropped into pulverizer, crush the size to 20-30mm, be positioned in clean container;
(2), gelatin extraction: will pack cooker into after Yak Bone accurate-metering, add the running water of 1.2 times of Yak Bone weight by duct type flowmeter, sealing dog-house, the mixed liquor that hot cooking becomes to contain grease and gelatin;
(3), collagen extract: after extraction finishes, draining valve at the bottom of unlatching cooker and the material inlet valve of receiver, observe visor, in the time that flowing to water oil content interface, mixed liquor closes in time receiver material inlet valve, open oil tank valve, grease is entered to oil tank, and grease separates completely with gelatin like this;
(4), complex enzyme hydrolysis: adopt the method for complex enzyme zymohydrolysis, carry out enzymolysis at receiver, can improve the degree of hydrolysis of protein, obtain the hydrolysate of how little molecular weight.Hydrolysis temperature is 48-52 ℃, and the time is 4-6 hour;
(5), the enzyme that goes out, sterilizing, isolated peptides liquid: after enzymolysis finishes, enzymolysis liquid is boiled 20 minutes, make enzyme-deactivating, make soup sterilizing simultaneously.Then leave standstill 30 minutes, at this moment hydrolyzate layering, upper strata is the slurry of the large molecule degeneration albumen of not enzymolysis and the enzyme of deactivation, lower floor is limpid transparent peptide liquid; Enzymolysis liquid is through vacuum filtration, and the filtrate of clarification enters filtrate tank, is peptide liquid;
(6), decoloration deodorization: the collagen peptidase hydrolyzed liquor making is above light yellow, take powdered active carbon as decoloration deodorization agent, enzymolysis liquid is carried out to decoloration deodorization;
(7), concentrated: temperature of charge is controlled at 80～82 ℃, and under 0.06～0.08Mpa vacuum, every batch of material concentration time of squeezing into inspissator is no more than 30 minutes.Be concentrated into Baume degrees 20～22B é, it is temporary that concentrate enters concentrate hold-up tank;
(8), spraying is dry: 45～50 ℃ of temperature of charge, Baume degrees 20～22B é, spray tower EAT is adjusted in 150～160 ℃ of scopes, leaving air temp, 80～90 ℃ of scopes, is dried to powder.
The complex enzyme adopting when complex enzyme hydrolysis is made up of protease, amylase, pectase, cellulase, zytase.
Claims (2)
1. a preparation method for Yak Bone collagen protein peptides, is characterized in that, technical process comprises raw material pre-treatment, gelatin extraction, and collagen extract, complex enzyme hydrolysis, the enzyme sterilizing of going out, isolated peptides liquid, decoloration deodorization, concentrate drying, concrete steps are:
(1), the pre-treatment of raw material: by the fresh or freezing Yak Bone of high-pressure cold water flushing, to remove foreign matter, then the Yak Bone of cleaning is dropped into pulverizer, crush the size to 20-30mm, be positioned in clean container;
(2), gelatin extraction: will pack cooker into after Yak Bone accurate-metering, add the running water of 1.2 times of Yak Bone weight by duct type flowmeter, sealing dog-house, the mixed liquor that hot cooking becomes to contain grease and gelatin;
(3), collagen extract: after extraction finishes, draining valve at the bottom of unlatching cooker and the material inlet valve of receiver, observe visor, in the time that flowing to water oil content interface, mixed liquor closes in time receiver material inlet valve, open oil tank valve, grease is entered to oil tank, and grease separates completely with gelatin like this;
(4), complex enzyme hydrolysis: adopt the method for complex enzyme zymohydrolysis, carry out enzymolysis at receiver, can improve the degree of hydrolysis of protein, obtain the hydrolysate of how little molecular weight.Hydrolysis temperature is 48-52 ℃, and the time is 4-6 hour;
(5), go out enzyme, sterilizing, isolated peptides liquid: after enzymolysis finishes, enzymolysis liquid is boiled 20 minutes, make enzyme-deactivating, make soup sterilizing simultaneously, then leave standstill 30 minutes, at this moment hydrolyzate layering, upper strata is the slurry of the large molecule degeneration albumen of not enzymolysis and the enzyme of deactivation, lower floor is limpid transparent peptide liquid; Enzymolysis liquid is through vacuum filtration, and the filtrate of clarification enters filtrate tank, is peptide liquid;
(6), decoloration deodorization: the collagen peptidase hydrolyzed liquor making is above light yellow, take powdered active carbon as decoloration deodorization agent, enzymolysis liquid is carried out to decoloration deodorization;
(7), concentrated: temperature of charge is controlled at 80～82 ℃, and under 0.06～0.08Mpa vacuum, every batch of material concentration time of squeezing into inspissator is no more than 30 minutes, is concentrated into Baume degrees 20～22B é, and it is temporary that concentrate enters concentrate hold-up tank;
(8), spraying is dry: 45～50 ℃ of temperature of charge, Baume degrees 20～22B é, spray tower EAT is adjusted in 150～160 ℃ of scopes, leaving air temp, 80～90 ℃ of scopes, is dried to powder.
2. the preparation method of a kind of Yak Bone collagen protein peptides as claimed in claim 1, is characterized in that, the complex enzyme adopting when complex enzyme hydrolysis is made up of protease, amylase, pectase, cellulase, zytase.
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| CN201310271584.1A CN103783254B (en) | 2013-07-02 | 2013-07-02 | A kind of preparation method of Os Bovis grunniens collagen peptide |
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| CN201310271584.1A CN103783254B (en) | 2013-07-02 | 2013-07-02 | A kind of preparation method of Os Bovis grunniens collagen peptide |
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| CN103783254B CN103783254B (en) | 2015-09-09 |
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| CN104173251A (en) * | 2014-09-15 | 2014-12-03 | 王书敏 | Whitening mask containing yak ossein peptide and preparation method of whitening mask |
| CN105018554A (en) * | 2015-07-30 | 2015-11-04 | 北京天肽生物科技有限公司 | Small molecule bovine bone collagen peptide and preparation method thereof |
| CN106480141A (en) * | 2015-08-28 | 2017-03-08 | 海南盛美诺生物技术有限公司 | A kind of preparation method of collagen |
| CN106619177A (en) * | 2016-11-25 | 2017-05-10 | 兰州大学 | Whitening and moisturizing cosmetic of white yak bone collagen protein peptide and preparation method of whitening and moisturizing cosmetic |
| CN107048418A (en) * | 2017-04-27 | 2017-08-18 | 长春市大桐生物科技有限公司 | A kind of peptide meal powder and preparation method thereof |
| CN107365379A (en) * | 2017-09-13 | 2017-11-21 | 甘肃中肽生物科技有限公司 | Ox bone collagen protein peptides extracting method |
| CN108796017A (en) * | 2018-06-22 | 2018-11-13 | 重庆申高生化制药股份有限公司 | Ox bone peptide and its enzymatic extraction method |
| CN108925742A (en) * | 2018-05-25 | 2018-12-04 | 余碧芝 | Bone collagen peptide and its production method with gel characteristic |
| CN108998490A (en) * | 2018-08-27 | 2018-12-14 | 王书敏 | The method and apparatus of small-molecular peptides is prepared using fresh yak bone |
| CN109247584A (en) * | 2017-07-16 | 2019-01-22 | 北京神飞航天应用技术研究院 | A kind of ox bone collagen protein peptide powder of suitable astronaut nutrition and preparation method thereof |
| CN109295145A (en) * | 2018-10-31 | 2019-02-01 | 西藏央金生态农牧科技有限公司 | A kind of preparation method of Ultra-low molecular weight yak collagen peptide |
| CN109380721A (en) * | 2018-09-29 | 2019-02-26 | 内蒙古蒙肽生物工程有限公司 | A kind of comprehensive composition and preparation method thereof for adjusting treating bone diseases |
| CN109419005A (en) * | 2017-08-25 | 2019-03-05 | 王亚军 | It is a kind of replenish the calcium, the composition of bone glue of yak former peptide of hypoglycemic and preparation method thereof |
| CN110024901A (en) * | 2019-04-30 | 2019-07-19 | 集美大学 | A kind of collagen peptide and its production method and process units and application |
| CN110089653A (en) * | 2016-12-12 | 2019-08-06 | 锡林郭勒盟肽好生物制品有限责任公司 | The formula and bone marrow peptide powder, preparation method thereof of reducing blood lipid polypeptide draft solid beverage |
| CN110669813A (en) * | 2019-10-28 | 2020-01-10 | 山西原生肽科技有限公司 | Yak rib small molecule peptide and extraction method thereof |
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