CN109247584A - A kind of ox bone collagen protein peptide powder of suitable astronaut nutrition and preparation method thereof - Google Patents
A kind of ox bone collagen protein peptide powder of suitable astronaut nutrition and preparation method thereof Download PDFInfo
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- CN109247584A CN109247584A CN201710580032.7A CN201710580032A CN109247584A CN 109247584 A CN109247584 A CN 109247584A CN 201710580032 A CN201710580032 A CN 201710580032A CN 109247584 A CN109247584 A CN 109247584A
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- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 159
- 239000000843 powder Substances 0.000 title claims abstract description 54
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 40
- 102000008186 Collagen Human genes 0.000 title claims abstract description 37
- 108010035532 Collagen Proteins 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 235000016709 nutrition Nutrition 0.000 title claims abstract description 22
- 230000035764 nutrition Effects 0.000 title claims abstract description 21
- 239000007787 solid Substances 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 235000013305 food Nutrition 0.000 claims abstract description 7
- 239000004519 grease Substances 0.000 claims abstract description 7
- 235000013361 beverage Nutrition 0.000 claims abstract description 6
- 230000037182 bone density Effects 0.000 claims abstract description 6
- 239000000839 emulsion Substances 0.000 claims abstract description 5
- 230000012010 growth Effects 0.000 claims abstract description 5
- 239000004816 latex Substances 0.000 claims abstract description 5
- 229920000126 latex Polymers 0.000 claims abstract description 5
- 235000014347 soups Nutrition 0.000 claims description 34
- 239000007788 liquid Substances 0.000 claims description 26
- 102000004190 Enzymes Human genes 0.000 claims description 15
- 108090000790 Enzymes Proteins 0.000 claims description 15
- 229940088598 enzyme Drugs 0.000 claims description 15
- 238000001035 drying Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 239000004365 Protease Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 5
- 235000015278 beef Nutrition 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 230000009849 deactivation Effects 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 5
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 5
- 238000000265 homogenisation Methods 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 239000013049 sediment Substances 0.000 claims description 5
- 238000004506 ultrasonic cleaning Methods 0.000 claims description 5
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 5
- 108090000526 Papain Proteins 0.000 claims description 4
- 102000057297 Pepsin A Human genes 0.000 claims description 4
- 108090000284 Pepsin A Proteins 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 4
- 230000002708 enhancing effect Effects 0.000 claims description 4
- 229940055729 papain Drugs 0.000 claims description 4
- 235000019834 papain Nutrition 0.000 claims description 4
- 229940111202 pepsin Drugs 0.000 claims description 4
- 235000019419 proteases Nutrition 0.000 claims description 4
- -1 trypsase Proteins 0.000 claims description 4
- 230000003064 anti-oxidating effect Effects 0.000 claims description 3
- 238000005056 compaction Methods 0.000 claims description 3
- 235000013402 health food Nutrition 0.000 claims description 3
- 239000007935 oral tablet Substances 0.000 claims description 3
- 229940096978 oral tablet Drugs 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000000796 flavoring agent Substances 0.000 claims description 2
- 235000019634 flavors Nutrition 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 4
- 230000036541 health Effects 0.000 abstract description 4
- 239000003963 antioxidant agent Substances 0.000 abstract description 3
- 230000003078 antioxidant effect Effects 0.000 abstract description 3
- 235000006708 antioxidants Nutrition 0.000 abstract description 3
- 239000012535 impurity Substances 0.000 abstract description 3
- 238000000926 separation method Methods 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 description 7
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- 150000001413 amino acids Chemical class 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
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- 229920001436 collagen Polymers 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 238000010298 pulverizing process Methods 0.000 description 4
- 206010065687 Bone loss Diseases 0.000 description 2
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 108010038983 glycyl-histidyl-lysine Proteins 0.000 description 2
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- 230000004792 oxidative damage Effects 0.000 description 2
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- 210000001519 tissue Anatomy 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108010016626 Dipeptides Proteins 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
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- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000027288 circadian rhythm Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
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- 239000006210 lotion Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
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- 229920001184 polypeptide Polymers 0.000 description 1
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- 235000000346 sugar Nutrition 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
- A23J3/342—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of collagen; of gelatin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses ox bone collagen protein peptide powders of a kind of suitable astronaut nutrition and preparation method thereof, comprising the following steps: (1) raw material preparation；(2) it is crushed；(3) it cleans；(4) dry；(5) it crushes for the first time；(6) it crushes for second；(7) autoclaving；(8) high-pressure homogeneous；(9) it digests；(10) it is concentrated；(11) it is spray-dried.The present invention is using ox bone as raw material, using grease in ultrasonic technology separation ox bone, it is carried out using super-pressure nanometer ram machine high-pressure homogeneous, the active peptide powder yield of preparation is high, product impurity content is few, granularity is small, it is easy to assimilate, and has the function of to enhance bone density, improves growth and development, anti-oxidant etc., can be widely applied in the spacefarer such as oral latex emulsion, solid beverage food or health care product.
Description
Technical field
The present invention relates to a kind of bone collagen Gly-His-Lys, and in particular to a kind of ox bone collagen albumen of suitable astronaut nutrition
Gly-His-Lys and preparation method thereof.
Background technique
Space flight is also known as space flight, space flight, space travel or space travel, refers to entrance, exploration, development and utilization
The general name of celestial body various activities other than space and the earth, effect is far beyond science and technology field, to country and state
Politics, economy, military affairs and the social life on border all generate extensively and far-reaching influence.The solar-system operation of the mankind be unable to do without spacefarer
Participation, but spacefarer is when executing space mission, is changed by weightlessness, noise, vibration, radiation, circadian rhythm, small space
The influence of equal space flight spy environmental factor, can bring adverse effect to the multiple physiological systems of body, in addition can seriously affect protein,
The requirement and metabolism of the nutrients such as fat, sugar and vitamin, wherein bone loss, vitamin situation are damaged, oxidative damage is most
The most important health problem of spacefarer, and only by implementing scientific and reasonable diet nutritional guarantee, energy needed for supplementing body
Amount and nutrient can mitigate adverse effect brought by space environment factor, maintain the health of spacefarer's body and mind, just can guarantee
Space mission smoothly completes.
Protein is to constitute all tissues of human body and cell as material base most basic and most important in vital movement
Important component, update, repairing, metabolism, the regulation of physiological function and the energy supply of tissue and cell all from
Do not open protein, it may be said that without protein just without life.The basic structural unit of protein is amino acid, physiological action
It is not only related with the composition type and quantity of amino acid, it is more related with the order of connection of amino acid, space structure etc..Active peptide is
Molecular structure between amino acid and protein, a kind of functional compounds for being formed by connecting with peptide bond, be natural by 20 kinds
Amino acid is formed and arrangement mode is constituted with different, from dipeptides (oligopeptides) to the total of linear or ring structure the polypeptide of complexity
Claim.Each active peptide has its unique composed structure, and different composed structures determines its different function.Study table
It is bright, there are many physiological functions using the active skin that collagen is prepared as feed degradation, such as inoxidizability, antibiotic property, promote calcium
Absorb, be depressured, be hypoglycemic etc..In addition, having document to disclose ox bone collagen protein peptides has proliferation promotion, antioxygen to human body osteocyte
The effects of change, antibacterial.Therefore, ox bone collagen protein peptides products application is prepared in space food, to spacefarer by raw material of ox bone
The health problems such as bone loss, oxidative damage have preferable food-therapy health-care efficacy before and after execution task.
Summary of the invention
In view of the above-mentioned problems, the present invention provides a kind of spacefarer ox bone collagen protein peptide powder and preparation method thereof,
Using ox bone as raw material, using grease in ultrasonic technology separation ox bone, high-pressure homogeneous, system is carried out using super-pressure nanometer ram machine
Standby active peptide powder yield is high, and product impurity content is few, granularity is small, is easy to assimilate, and has enhancing bone density, improves life
The functions such as development, anti-oxidant are produced, can be widely applied in the spacefarer such as oral latex emulsion, solid beverage food or health care product.
A kind of preparation method of the ox bone collagen protein peptide powder of suitable astronaut nutrition, it is characterised in that including following step
It is rapid:
(1) raw material preparation: choosing the fresh ox bone for just butchering removing, clear up, weed out the beef adhered on bone, standby
With；
(2) it is crushed: ox bone being crushed using bone cutter, bone mud machine, obtains ox bone mud；
(3) it cleans: being that saline solution is added into ox bone mud in 1:2~5kg/kg by feed liquid mass ratio, be uniformly mixed, it
Be placed in ultrasonic cleaning equipment, under conditions of 40~60 DEG C of temperature, 200~300KW of ultrasonic power handle 10~
30min is centrifuged in 4000~6000r/min later, takes the ox bone mud of lower sediment spare；
(4) dry: the ox bone mud after cleaning to be subjected to vacuum freeze drying, drying to moisture content is lower than 15%；
(5) it crushes for the first time: the ox bone mud after drying being crushed using vacuum pulverizer, is crushed to the granularity of ox bone
Less than 50mm, ox bone just powder is obtained；
(6) it second crushes: further pulverizing powder at the beginning of ox bone, be crushed to the granularity of ox bone less than 100 μm,
Obtain ox bone Ultramicro-powder；
(7) autoclaving: being added distilled water by solid-liquid ratio mass ratio 1:5~10kg/kg into ox bone Ultramicro-powder, sufficiently mixed
It closes uniformly, autoclaving is carried out under the pressure of 0.115~0.125MPa, later natural cooling, upper layer grease is separated, under collection
Layer ox bone soup, it is spare；
(8) high-pressure homogeneous: ox bone soup to be crossed into 200 meshes, lower ox bone soup will be sieved by collecting, and be clashed using super-pressure nanometer
Machine carries out incrementally super-high-pressure homogenization and handles, method particularly includes: lower ox bone soup will be first sieved in the condition of 80~100MPa of pressure
Lower processing 1 time is later handled pressure increase to 110~130MPa 1 time, finally by pressure increase to 150~180MPa processing 1
~3 times to get nanosizing ox bone soup；
(9) it digests: the pH of nanosizing ox bone soup being adjusted to 6.5~7.8, enzyme solution is added and carries out heat preservation enzymatic hydrolysis, later
Enzyme deactivation obtains Galbitang enzymolysis liquid；
(10) be concentrated: it is 15%~25% that Galbitang enzymolysis liquid, which is concentrated into solid content,；
(11) it is spray-dried: the Galbitang enzymolysis liquid after concentration is spray-dried to get ox bone collagen protein peptide powder.
Preferably, enzyme used in the enzymolysis step include pepsin, trypsase, papain,
Flavor protease.
Preferably, the average volume particle diameter of the nanosizing ox bone soup obtained after the high-pressure homogeneous processing is less than
80nm。
Using the ox bone collagen protein peptide powder of the suitable astronaut nutrition of above method preparation.
Preferably, the ox bone collagen protein peptide powder of the suitable astronaut nutrition is applied to preparation spacefarer's mouth
Take lotion, solid beverage, high-energy compaction food, oral tablet.
Preferably, the ox bone collagen protein peptide powder of the suitable astronaut nutrition, which is applied to preparation, has enhancing bone
Density, the spacefarer's health food for improving growth and development, anti-oxidation function.
Preferably, the molecular weight of the ox bone collagen protein peptide powder of the suitable astronaut nutrition is 2000~3000
Dalton.
Preferably, the fat content of the ox bone collagen protein peptide powder of the suitable astronaut nutrition is less than
0.05%.
The invention has the advantages that:
It 1), can be by the oil in ox bone mud using ultrasonic technology and autoclaving after ox bone is prepared skeletonization mud by the present invention
Aliphatic radical is originally separated and is removed, and the active peptide product of preparation does not bond, and is substantially free of the impurity such as fat, cholesterol；
2) ox bone is prepared into micron-class superfine bovine bone powder first using freeze-drying, then by crushing step by step by the present invention, so
Autoclaving decomposition afterwards prepares collagen, is handled after adding water quenched using super-pressure nanometer ram machine, average external volume can be obtained
Partial size reaches nanoscale ox bone soup, is digested later as raw material, ox bone soup can be significantly increased and contact with enzyme solution
Reaction site, increase enzymolysis efficiency, the yield of ox bone protein peptides is substantially improved；
3) it is powdery that the present invention, which adopts product, and granularity is easy to assimilate up to nanoscale, processes strong applicability, and have
Enhance bone density, improve the functions such as growth and development, anti-oxidant, can be widely applied to prepare the space flight such as oral latex emulsion, solid beverage
Member's food or health care product.
Specific embodiment
The present invention is described further With reference to embodiment, it is pointed out that following implementation is only
It is the indicative explaination that the form to enumerate is the present invention, but protection scope of the present invention is not limited to that, Suo Youben
The technical staff in field each falls within protection scope of the present invention with the equivalent replacement that spirit of the invention is the present invention.
Embodiment 1
A kind of preparation method of the ox bone collagen protein peptide powder of suitable astronaut nutrition, comprising the following steps:
(1) raw material preparation: choosing the fresh ox bone for just butchering removing, clear up, weed out the beef adhered on bone, standby
With；
(2) it is crushed: ox bone being crushed using bone cutter, bone mud machine, obtains ox bone mud；
(3) it cleans: being that saline solution is added into ox bone mud in 1:2~5kg/kg by feed liquid mass ratio, be uniformly mixed, it
Be placed in ultrasonic cleaning equipment, under conditions of 40~60 DEG C of temperature, 200~300KW of ultrasonic power handle 10~
30min is centrifuged in 4000~6000r/min later, takes the ox bone mud of lower sediment spare；
(4) dry: the ox bone mud after cleaning to be subjected to vacuum freeze drying, drying to moisture content is lower than 15%；
(5) it crushes for the first time: the ox bone mud after drying being crushed using vacuum pulverizer, is crushed to the granularity of ox bone
Less than 50mm, ox bone just powder is obtained；
(6) it second crushes: further pulverizing powder at the beginning of ox bone, be crushed to the granularity of ox bone less than 100 μm,
Obtain ox bone Ultramicro-powder；
(7) autoclaving: being added distilled water by solid-liquid ratio mass ratio 1:5~10kg/kg into ox bone Ultramicro-powder, sufficiently mixed
It closes uniformly, autoclaving is carried out under the pressure of 0.115~0.125MPa, later natural cooling, upper layer grease is separated, under collection
Layer ox bone soup, it is spare；
(8) high-pressure homogeneous: ox bone soup to be crossed into 200 meshes, lower ox bone soup will be sieved by collecting, and be clashed using super-pressure nanometer
Machine carries out incrementally super-high-pressure homogenization and handles, method particularly includes: lower ox bone soup will be first sieved in the condition of 80~100MPa of pressure
Lower processing 1 time is later handled pressure increase to 110~130MPa 1 time, finally by pressure increase to 150~180MPa processing 1
~3 times to get nanosizing ox bone soup；
(9) it digests: the pH of nanosizing ox bone soup being adjusted to 6.5~7.8, enzyme solution is added and carries out heat preservation enzymatic hydrolysis, later
Enzyme deactivation obtains Galbitang enzymolysis liquid；
(10) be concentrated: it is 15%~25% that Galbitang enzymolysis liquid, which is concentrated into solid content,；
(11) it is spray-dried: the Galbitang enzymolysis liquid after concentration is spray-dried to get ox bone collagen protein peptide powder.
Embodiment 2
A kind of preparation method of the ox bone collagen protein peptide powder of suitable astronaut nutrition, comprising the following steps:
(1) raw material preparation: choosing the fresh ox bone for just butchering removing, clear up, weed out the beef adhered on bone, standby
With；
(2) it is crushed: ox bone being crushed using bone cutter, bone mud machine, obtains ox bone mud；
(3) it cleans: being that saline solution is added into ox bone mud in 1:2~5kg/kg by feed liquid mass ratio, be uniformly mixed, it
Be placed in ultrasonic cleaning equipment, under conditions of 40~60 DEG C of temperature, 200~300KW of ultrasonic power handle 10~
30min is centrifuged in 4000~6000r/min later, takes the ox bone mud of lower sediment spare；
(4) dry: the ox bone mud after cleaning to be subjected to vacuum freeze drying, drying to moisture content is lower than 15%；
(5) it crushes for the first time: the ox bone mud after drying being crushed using vacuum pulverizer, is crushed to the granularity of ox bone
Less than 50mm, ox bone just powder is obtained；
(6) it second crushes: further pulverizing powder at the beginning of ox bone, be crushed to the granularity of ox bone less than 100 μm,
Obtain ox bone Ultramicro-powder；
(7) autoclaving: being added distilled water by solid-liquid ratio mass ratio 1:5~10kg/kg into ox bone Ultramicro-powder, sufficiently mixed
It closes uniformly, autoclaving is carried out under the pressure of 0.115~0.125MPa, later natural cooling, upper layer grease is separated, under collection
Layer ox bone soup, it is spare；
(8) high-pressure homogeneous: ox bone soup to be crossed into 200 meshes, lower ox bone soup will be sieved by collecting, and be clashed using super-pressure nanometer
Machine carries out incrementally super-high-pressure homogenization and handles, method particularly includes: lower ox bone soup will be first sieved in the condition of 80~100MPa of pressure
Lower processing 1 time is later handled pressure increase to 110~130MPa 1 time, finally by pressure increase to 150~180MPa processing 1
~3 times to get nanosizing ox bone soup；
(9) it digests: the pH of nanosizing ox bone soup being adjusted to 6.5~7.8, enzyme solution is added and carries out heat preservation enzymatic hydrolysis, later
Enzyme deactivation obtains Galbitang enzymolysis liquid；Enzyme used in the enzymolysis step includes pepsin, trypsase, papain, wind
Taste protease；
(10) be concentrated: it is 15%~25% that Galbitang enzymolysis liquid, which is concentrated into solid content,；
(11) it is spray-dried: the Galbitang enzymolysis liquid after concentration is spray-dried to get ox bone collagen protein peptide powder.
Embodiment 3
A kind of preparation method of the ox bone collagen protein peptide powder of suitable astronaut nutrition, comprising the following steps:
(1) raw material preparation: choosing the fresh ox bone for just butchering removing, clear up, weed out the beef adhered on bone, standby
With；
(2) it is crushed: ox bone being crushed using bone cutter, bone mud machine, obtains ox bone mud；
(3) it cleans: being that saline solution is added into ox bone mud in 1:2~5kg/kg by feed liquid mass ratio, be uniformly mixed, it
Be placed in ultrasonic cleaning equipment, under conditions of 40~60 DEG C of temperature, 200~300KW of ultrasonic power handle 10~
30min is centrifuged in 4000~6000r/min later, takes the ox bone mud of lower sediment spare；
(4) dry: the ox bone mud after cleaning to be subjected to vacuum freeze drying, drying to moisture content is lower than 15%；
(5) it crushes for the first time: the ox bone mud after drying being crushed using vacuum pulverizer, is crushed to the granularity of ox bone
Less than 50mm, ox bone just powder is obtained；
(6) it second crushes: further pulverizing powder at the beginning of ox bone, be crushed to the granularity of ox bone less than 100 μm,
Obtain ox bone Ultramicro-powder；
(7) autoclaving: being added distilled water by solid-liquid ratio mass ratio 1:5~10kg/kg into ox bone Ultramicro-powder, sufficiently mixed
It closes uniformly, autoclaving is carried out under the pressure of 0.115~0.125MPa, later natural cooling, upper layer grease is separated, under collection
Layer ox bone soup, it is spare；
(8) high-pressure homogeneous: ox bone soup to be crossed into 200 meshes, lower ox bone soup will be sieved by collecting, and be clashed using super-pressure nanometer
Machine carries out incrementally super-high-pressure homogenization and handles, method particularly includes: lower ox bone soup will be first sieved in the condition of 80~100MPa of pressure
Lower processing 1 time is later handled pressure increase to 110~130MPa 1 time, finally by pressure increase to 150~180MPa processing 1
~3 nanosizing ox bone soup to get average volume particle diameter less than 80nm；
(9) it digests: the pH of nanosizing ox bone soup being adjusted to 6.5~7.8, enzyme solution is added and carries out heat preservation enzymatic hydrolysis, later
Enzyme deactivation obtains Galbitang enzymolysis liquid；Enzyme used in the enzymolysis step includes pepsin, trypsase, papain, wind
Taste protease；
(10) be concentrated: it is 15%~25% that Galbitang enzymolysis liquid, which is concentrated into solid content,；
(11) it is spray-dried: the Galbitang enzymolysis liquid after concentration is spray-dried to get ox bone collagen protein peptide powder.
Embodiment 4
Using the ox bone collagen protein peptide powder of any the method preparation of embodiment 1 to 3.
Embodiment 5
Ox bone collagen protein peptide powder of the present invention can be applied to preparation spacefarer's oral latex emulsion, solid beverage, high-energy compaction
Food, oral tablet；Additive amount is 5%~30%.
Embodiment 6
Ox bone collagen protein peptide powder of the present invention can prepare with enhancing bone density, improve growth and development, anti-oxidation function
Spacefarer's health food.
Embodiment 7
The molecular weight of ox bone collagen protein peptide powder of the present invention is 2000~3000 dalton.
Embodiment 8
The fat content of ox bone collagen protein peptide powder of the present invention is less than 0.05%.
Claims (8)
1. a kind of preparation method of the ox bone collagen protein peptide powder of suitable astronaut nutrition, it is characterised in that the following steps are included:
(1) raw material preparation: choosing the fresh ox bone for just butchering removing, clear up, weed out the beef adhered on bone, spare；
(2) it is crushed: ox bone being crushed using bone cutter, bone mud machine, obtains ox bone mud；
(3) clean: by feed liquid mass ratio be 1:2~5kg/kg saline solution is added into ox bone mud, be uniformly mixed, postposition
In in ultrasonic cleaning equipment, 10~30min is handled under conditions of 40~60 DEG C of temperature, 200~300KW of ultrasonic power, it
It is centrifuged afterwards in 4000~6000r/min, takes the ox bone mud of lower sediment spare；
(4) dry: the ox bone mud after cleaning to be subjected to vacuum freeze drying, drying to moisture content is lower than 15%；
(5) it crushes: the ox bone mud after drying being crushed using vacuum pulverizer, the granularity for being crushed to ox bone is less than for the first time
50mm obtains ox bone just powder；
(6) it crushes for second: further powder at the beginning of ox bone being pulverized, the granularity of ox bone is crushed to less than 100 μm, obtains ox
Bone Ultramicro-powder；
(7) autoclaving: being added distilled water by solid-liquid ratio mass ratio 1:5~10kg/kg into ox bone Ultramicro-powder, is sufficiently mixed
It is even, autoclaving is carried out under the pressure of 0.115~0.125MPa, later natural cooling, separate upper layer grease, collect lower layer ox
Bone soup liquid, it is spare；
(8) high-pressure homogeneous: ox bone soup is crossed into 200 meshes, lower ox bone soup will be sieved by collecting, using super-pressure nanometer ram machine into
Incrementally super-high-pressure homogenization is handled row, method particularly includes: lower ox bone soup will be first sieved under conditions of 80~100MPa of pressure
Reason 1 time is later handled pressure increase to 110~130MPa 1 time, finally by pressure increase to 150~180MPa processing 1~3
It is secondary to get nanosizing ox bone soup；
(9) it digests: the pH of nanosizing ox bone soup is adjusted to 6.5~7.8, enzyme solution is added and carries out heat preservation enzymatic hydrolysis, enzyme deactivation later,
Obtain Galbitang enzymolysis liquid；
(10) be concentrated: it is 15%~25% that Galbitang enzymolysis liquid, which is concentrated into solid content,；
(11) it is spray-dried: the Galbitang enzymolysis liquid after concentration is spray-dried to get ox bone collagen protein peptide powder.
2. a kind of preparation method of the ox bone collagen protein peptide powder of suitable astronaut nutrition as described in claim 1, feature
It is that enzyme used in the enzymolysis step includes pepsin, trypsase, papain, flavor protease.
3. a kind of preparation method of the ox bone collagen protein peptide powder of suitable astronaut nutrition as described in claim 1, feature
It is that the average volume particle diameter of the nanosizing ox bone soup obtained after the high-pressure homogeneous processing is less than 80nm.
4. the ox bone collagen protein peptide powder of the suitable astronaut nutrition using any the method preparation of claims 1 to 3.
5. the ox bone collagen protein peptide powder of suitable astronaut nutrition as claimed in claim 4, it is characterised in that be applied to preparation
Spacefarer's oral latex emulsion, solid beverage, high-energy compaction food, oral tablet.
6. the ox bone collagen protein peptide powder of suitable astronaut nutrition as claimed in claim 4, it is characterised in that be applied to preparation
With the spacefarer's health food for enhancing bone density, improving growth and development, anti-oxidation function.
7. the ox bone collagen protein peptide powder of suitable astronaut nutrition as claimed in claim 4, it is characterised in that molecular weight is
2000~3000 dalton.
8. the ox bone collagen protein peptide powder of suitable astronaut nutrition as claimed in claim 4, it is characterised in that fat content is small
In 0.05%.
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