CN114072519B - 新型β淀粉酶及其应用和制备方法 - Google Patents

新型β淀粉酶及其应用和制备方法 Download PDF

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CN114072519B
CN114072519B CN202080028731.0A CN202080028731A CN114072519B CN 114072519 B CN114072519 B CN 114072519B CN 202080028731 A CN202080028731 A CN 202080028731A CN 114072519 B CN114072519 B CN 114072519B
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amylase
beta
enzyme
starch
seq
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CN114072519A (zh
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炭谷顺一
谷修治
川口刚司
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Public University Legal Person Osaka
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2425—Beta-amylase (3.2.1.2)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
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    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/22—Preparation of compounds containing saccharide radicals produced by the action of a beta-amylase, e.g. maltose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12R2001/07—Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202080028731.0A 2019-04-15 2020-04-14 新型β淀粉酶及其应用和制备方法 Active CN114072519B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2019-077041 2019-04-15
JP2019077041 2019-04-15
JP2019-145697 2019-08-07
JP2019145697 2019-08-07
PCT/JP2020/016434 WO2020213604A1 (ja) 2019-04-15 2020-04-14 新規βアミラーゼ及びその利用・製造法

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CN114072519A CN114072519A (zh) 2022-02-18
CN114072519B true CN114072519B (zh) 2025-03-04

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US (1) US12473542B2 (https=)
JP (1) JP7649547B2 (https=)
CN (1) CN114072519B (https=)
WO (1) WO2020213604A1 (https=)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107058263A (zh) * 2017-01-26 2017-08-18 福建福大百特生物科技有限公司 一种新型β‑淀粉酶的高效制备方法
CN107164345A (zh) * 2017-07-06 2017-09-15 江南大学 一种热稳定性提高的β‑淀粉酶突变体

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4867487A (https=) 1971-12-17 1973-09-14
JPS61282073A (ja) * 1985-06-07 1986-12-12 Nakano Vinegar Co Ltd 新規アミラ−ゼ，該アミラ−ゼをコ−ドする遺伝子を含む組換えプラスミド，該プラスミドにより形質転換された微生物および該微生物による新規アミラ−ゼの製造法
JPS6279746A (ja) 1985-10-01 1987-04-13 Showa Sangyo Kk でんぷん質食品の老化を防止する方法
DK160563C (da) 1986-02-19 1991-09-02 Novo Industri As Beta-amylase, fremgangsmaade til dens fremstilling samt praeparat indeholdende beta-amylasen
CN101153276A (zh) * 2006-09-27 2008-04-02 吴襟 一种β-淀粉酶及其编码基因与生产方法
DK2454942T3 (en) 2009-07-17 2018-07-30 Amano Enzyme Inc PROCEDURE FOR IMPROVING FOODS USING BETA AMYLASE
CN107254459A (zh) * 2017-06-29 2017-10-17 中国科学院青岛生物能源与过程研究所 耐热β‑淀粉酶‑海藻糖合成酶融合酶、其表达基因以及分泌该融合酶的工程菌与应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107058263A (zh) * 2017-01-26 2017-08-18 福建福大百特生物科技有限公司 一种新型β‑淀粉酶的高效制备方法
CN107164345A (zh) * 2017-07-06 2017-09-15 江南大学 一种热稳定性提高的β‑淀粉酶突变体

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US12473542B2 (en) 2025-11-18
JP7649547B2 (ja) 2025-03-21
US20230077057A1 (en) 2023-03-09
WO2020213604A1 (ja) 2020-10-22
CN114072519A (zh) 2022-02-18
JPWO2020213604A1 (https=) 2020-10-22

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