CN114040985A - 用于对多核苷酸进行测序的方法 - Google Patents

用于对多核苷酸进行测序的方法 Download PDF

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Publication number
CN114040985A
CN114040985A CN202180004113.7A CN202180004113A CN114040985A CN 114040985 A CN114040985 A CN 114040985A CN 202180004113 A CN202180004113 A CN 202180004113A CN 114040985 A CN114040985 A CN 114040985A
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sequencing
strand
template
extension reaction
immobilized
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Chinese (zh)
Inventor
J·佩斯
K·博亚诺维奇马查多
P·麦金纳尼
J·M·布特尔
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Inmair Ltd
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Inmair Ltd
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/101—Linear amplification, i.e. non exponential
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/101—Primer extension
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/137—Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step
    • C12Q2537/1376—Displacement by an enzyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202180004113.7A 2020-03-09 2021-03-09 用于对多核苷酸进行测序的方法 Pending CN114040985A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062987035P 2020-03-09 2020-03-09
US62/987,035 2020-03-09
PCT/EP2021/055939 WO2021180733A1 (en) 2020-03-09 2021-03-09 Methods for sequencing polynucleotides

Publications (1)

Publication Number Publication Date
CN114040985A true CN114040985A (zh) 2022-02-11

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CN202180004113.7A Pending CN114040985A (zh) 2020-03-09 2021-03-09 用于对多核苷酸进行测序的方法

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US (1) US20220316003A1 (https=)
EP (1) EP3969618B1 (https=)
JP (1) JP7817834B2 (https=)
KR (1) KR20220151101A (https=)
CN (1) CN114040985A (https=)
AU (1) AU2021234084A1 (https=)
CA (1) CA3145445A1 (https=)
DK (1) DK3969618T3 (https=)
ES (1) ES2965266T3 (https=)
FI (1) FI3969618T3 (https=)
PL (1) PL3969618T3 (https=)
PT (1) PT3969618T (https=)
WO (1) WO2021180733A1 (https=)

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CN112654714B (zh) 2018-12-17 2024-10-11 伊卢米纳剑桥有限公司 用于测序的引物寡核苷酸
BR112022026860A2 (pt) * 2020-09-14 2023-04-11 Illumina Inc Composições e métodos para amplificar polinucleotídeos
WO2023126457A1 (en) * 2021-12-29 2023-07-06 Illumina Cambridge Ltd. Methods of nucleic acid sequencing using surface-bound primers
US20230348973A1 (en) * 2022-03-31 2023-11-02 Illumina Cambridge Limited Paired-end re-synthesis using blocked p5 primers
WO2024249591A1 (en) * 2023-05-31 2024-12-05 Illumina, Inc. Methods for double-stranded sequencing by synthesis

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CN101415839A (zh) * 2006-02-08 2009-04-22 亿明达剑桥有限公司 对多核苷酸模板进行测序的方法
CN102344967A (zh) * 2011-11-02 2012-02-08 东南大学 一种缩短dna模板的dna测序方法及其应用
EP2470699A2 (en) * 2009-08-27 2012-07-04 LG Electronics Inc. Control method of laundry machine
US20140329698A1 (en) * 2007-02-02 2014-11-06 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple polynucleotide templates
EP3155122A1 (en) * 2014-06-11 2017-04-19 Illumina Cambridge Limited Methods for estimating cluster numbers
CN107532219A (zh) * 2015-03-25 2018-01-02 爱克瑟拉有限公司 使用链置换聚合酶的固相靶核酸捕获和复制
CN109642251A (zh) * 2016-06-30 2019-04-16 卢米拉特英国有限公司 核酸扩增过程中的改进或与核酸扩增过程相关的改进

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CN101415839A (zh) * 2006-02-08 2009-04-22 亿明达剑桥有限公司 对多核苷酸模板进行测序的方法
WO2008041002A2 (en) * 2006-10-06 2008-04-10 Illumina Cambridge Limited Method for sequencing a polynucleotide template
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CN102344967A (zh) * 2011-11-02 2012-02-08 东南大学 一种缩短dna模板的dna测序方法及其应用
EP3155122A1 (en) * 2014-06-11 2017-04-19 Illumina Cambridge Limited Methods for estimating cluster numbers
CN107532219A (zh) * 2015-03-25 2018-01-02 爱克瑟拉有限公司 使用链置换聚合酶的固相靶核酸捕获和复制
CN109642251A (zh) * 2016-06-30 2019-04-16 卢米拉特英国有限公司 核酸扩增过程中的改进或与核酸扩增过程相关的改进

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CA3145445A1 (en) 2021-09-16
PL3969618T3 (pl) 2024-01-03
US20220316003A1 (en) 2022-10-06
WO2021180733A1 (en) 2021-09-16
DK3969618T3 (da) 2023-10-16
AU2021234084A1 (en) 2022-01-06
PT3969618T (pt) 2023-11-15
JP7817834B2 (ja) 2026-02-19
JP2023517157A (ja) 2023-04-24
ES2965266T3 (es) 2024-04-11
EP3969618A1 (en) 2022-03-23
EP3969618B1 (en) 2023-09-13
FI3969618T3 (fi) 2023-10-18
KR20220151101A (ko) 2022-11-14

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