AU2021234084A1 - Methods for sequencing polynucleotides - Google Patents
Methods for sequencing polynucleotides Download PDFInfo
- Publication number
- AU2021234084A1 AU2021234084A1 AU2021234084A AU2021234084A AU2021234084A1 AU 2021234084 A1 AU2021234084 A1 AU 2021234084A1 AU 2021234084 A AU2021234084 A AU 2021234084A AU 2021234084 A AU2021234084 A AU 2021234084A AU 2021234084 A1 AU2021234084 A1 AU 2021234084A1
- Authority
- AU
- Australia
- Prior art keywords
- sequencing
- strand
- template
- immobilised
- extension reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/101—Linear amplification, i.e. non exponential
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
- C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
- C12Q2533/101—Primer extension
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/137—Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step
- C12Q2537/1376—Displacement by an enzyme
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062987035P | 2020-03-09 | 2020-03-09 | |
| US62/987,035 | 2020-03-09 | ||
| PCT/EP2021/055939 WO2021180733A1 (en) | 2020-03-09 | 2021-03-09 | Methods for sequencing polynucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2021234084A1 true AU2021234084A1 (en) | 2022-01-06 |
Family
ID=74947346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2021234084A Pending AU2021234084A1 (en) | 2020-03-09 | 2021-03-09 | Methods for sequencing polynucleotides |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20220316003A1 (https=) |
| EP (1) | EP3969618B1 (https=) |
| JP (1) | JP7817834B2 (https=) |
| KR (1) | KR20220151101A (https=) |
| CN (1) | CN114040985A (https=) |
| AU (1) | AU2021234084A1 (https=) |
| CA (1) | CA3145445A1 (https=) |
| DK (1) | DK3969618T3 (https=) |
| ES (1) | ES2965266T3 (https=) |
| FI (1) | FI3969618T3 (https=) |
| PL (1) | PL3969618T3 (https=) |
| PT (1) | PT3969618T (https=) |
| WO (1) | WO2021180733A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112654714B (zh) | 2018-12-17 | 2024-10-11 | 伊卢米纳剑桥有限公司 | 用于测序的引物寡核苷酸 |
| BR112022026860A2 (pt) * | 2020-09-14 | 2023-04-11 | Illumina Inc | Composições e métodos para amplificar polinucleotídeos |
| WO2023126457A1 (en) * | 2021-12-29 | 2023-07-06 | Illumina Cambridge Ltd. | Methods of nucleic acid sequencing using surface-bound primers |
| US20230348973A1 (en) * | 2022-03-31 | 2023-11-02 | Illumina Cambridge Limited | Paired-end re-synthesis using blocked p5 primers |
| WO2024249591A1 (en) * | 2023-05-31 | 2024-12-05 | Illumina, Inc. | Methods for double-stranded sequencing by synthesis |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5302509A (en) | 1989-08-14 | 1994-04-12 | Beckman Instruments, Inc. | Method for sequencing polynucleotides |
| US6537764B1 (en) | 1995-01-19 | 2003-03-25 | Children's Medical Center Corporation | Method of identifying inhibitors of C—C chemokine receptor 3 |
| JP2001517948A (ja) | 1997-04-01 | 2001-10-09 | グラクソ、グループ、リミテッド | 核酸配列決定法 |
| JP2002503954A (ja) | 1997-04-01 | 2002-02-05 | グラクソ、グループ、リミテッド | 核酸増幅法 |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| WO2001079553A1 (en) | 2000-04-14 | 2001-10-25 | Lynx Therapeutics, Inc. | Method and compositions for ordering restriction fragments |
| WO2002006456A1 (en) | 2000-07-13 | 2002-01-24 | Invitrogen Corporation | Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix |
| JP2005518811A (ja) | 2002-03-05 | 2005-06-30 | ソレックサ リミテッド | 表現型に関連するゲノム全域での配列変化を決定するための方法 |
| WO2004018497A2 (en) | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| US20060024681A1 (en) | 2003-10-31 | 2006-02-02 | Agencourt Bioscience Corporation | Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof |
| GB0400584D0 (en) | 2004-01-12 | 2004-02-11 | Solexa Ltd | Nucleic acid chacterisation |
| ES2404311T3 (es) | 2005-04-12 | 2013-05-27 | 454 Life Sciences Corporation | Métodos para determinar variantes de secuencias usando secuenciación ultraprofunda |
| US8428882B2 (en) | 2005-06-14 | 2013-04-23 | Agency For Science, Technology And Research | Method of processing and/or genome mapping of diTag sequences |
| GB0514910D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Method for sequencing a polynucleotide template |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| SG169356A1 (en) | 2006-02-08 | 2011-03-30 | Illumina Cambridge Ltd | Method for sequencing a polynucleotide template |
| WO2007107710A1 (en) | 2006-03-17 | 2007-09-27 | Solexa Limited | Isothermal methods for creating clonal single molecule arrays |
| US7754429B2 (en) * | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
| WO2008093098A2 (en) * | 2007-02-02 | 2008-08-07 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| PT2669387T (pt) * | 2009-08-25 | 2016-09-20 | Illumina Inc | Métodos para selecção e amplificação de polinucleótidos |
| EP2470699B1 (en) * | 2009-08-27 | 2017-10-04 | LG Electronics Inc. | Control method of laundry machine |
| EP2718465B1 (en) | 2011-06-09 | 2022-04-13 | Illumina, Inc. | Method of making an analyte array |
| CN102344967B (zh) * | 2011-11-02 | 2014-10-08 | 东南大学 | 一种缩短dna模板的dna测序方法及其应用 |
| US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
| CN107760772B (zh) * | 2012-08-24 | 2022-02-01 | 生命技术公司 | 用于核酸配对末端测序的方法、组合物、系统、仪器和试剂盒 |
| JP6451046B2 (ja) * | 2013-12-17 | 2019-01-16 | 東ソー株式会社 | 核酸増幅方法および当該方法を利用した核酸増幅試薬 |
| GB201410420D0 (en) * | 2014-06-11 | 2014-07-23 | Illumina Cambridge Ltd | Methods for estimating cluster numbers |
| JP6963505B2 (ja) * | 2015-03-25 | 2021-11-10 | アングル ヨーロッパ リミテッド | 鎖置換ポリメラーゼを用いた固相核酸標的捕捉及び複製 |
| GB201611469D0 (en) * | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
-
2021
- 2021-03-09 PL PL21712731.5T patent/PL3969618T3/pl unknown
- 2021-03-09 CA CA3145445A patent/CA3145445A1/en active Pending
- 2021-03-09 AU AU2021234084A patent/AU2021234084A1/en active Pending
- 2021-03-09 KR KR1020217043200A patent/KR20220151101A/ko active Pending
- 2021-03-09 WO PCT/EP2021/055939 patent/WO2021180733A1/en not_active Ceased
- 2021-03-09 PT PT217127315T patent/PT3969618T/pt unknown
- 2021-03-09 US US17/618,836 patent/US20220316003A1/en active Pending
- 2021-03-09 EP EP21712731.5A patent/EP3969618B1/en active Active
- 2021-03-09 FI FIEP21712731.5T patent/FI3969618T3/fi active
- 2021-03-09 ES ES21712731T patent/ES2965266T3/es active Active
- 2021-03-09 DK DK21712731.5T patent/DK3969618T3/da active
- 2021-03-09 JP JP2021577994A patent/JP7817834B2/ja active Active
- 2021-03-09 CN CN202180004113.7A patent/CN114040985A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN114040985A (zh) | 2022-02-11 |
| CA3145445A1 (en) | 2021-09-16 |
| PL3969618T3 (pl) | 2024-01-03 |
| US20220316003A1 (en) | 2022-10-06 |
| WO2021180733A1 (en) | 2021-09-16 |
| DK3969618T3 (da) | 2023-10-16 |
| PT3969618T (pt) | 2023-11-15 |
| JP7817834B2 (ja) | 2026-02-19 |
| JP2023517157A (ja) | 2023-04-24 |
| ES2965266T3 (es) | 2024-04-11 |
| EP3969618A1 (en) | 2022-03-23 |
| EP3969618B1 (en) | 2023-09-13 |
| FI3969618T3 (fi) | 2023-10-18 |
| KR20220151101A (ko) | 2022-11-14 |
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