PT3969618T - Métodos para sequenciação de polinucleótidos - Google Patents

Métodos para sequenciação de polinucleótidos

Info

Publication number
PT3969618T
PT3969618T PT217127315T PT21712731T PT3969618T PT 3969618 T PT3969618 T PT 3969618T PT 217127315 T PT217127315 T PT 217127315T PT 21712731 T PT21712731 T PT 21712731T PT 3969618 T PT3969618 T PT 3969618T
Authority
PT
Portugal
Prior art keywords
methods
sequencing polynucleotides
polynucleotides
sequencing
Prior art date
Application number
PT217127315T
Other languages
English (en)
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of PT3969618T publication Critical patent/PT3969618T/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/101Temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/101Linear amplification, i.e. non exponential
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/101Primer extension
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/137Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step
    • C12Q2537/1376Displacement by an enzyme
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PT217127315T 2020-03-09 2021-03-09 Métodos para sequenciação de polinucleótidos PT3969618T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202062987035P 2020-03-09 2020-03-09

Publications (1)

Publication Number Publication Date
PT3969618T true PT3969618T (pt) 2023-11-15

Family

ID=74947346

Family Applications (1)

Application Number Title Priority Date Filing Date
PT217127315T PT3969618T (pt) 2020-03-09 2021-03-09 Métodos para sequenciação de polinucleótidos

Country Status (13)

Country Link
US (1) US20220316003A1 (pt)
EP (1) EP3969618B1 (pt)
JP (1) JP2023517157A (pt)
KR (1) KR20220151101A (pt)
CN (1) CN114040985A (pt)
AU (1) AU2021234084A1 (pt)
CA (1) CA3145445A1 (pt)
DK (1) DK3969618T3 (pt)
ES (1) ES2965266T3 (pt)
FI (1) FI3969618T3 (pt)
PL (1) PL3969618T3 (pt)
PT (1) PT3969618T (pt)
WO (1) WO2021180733A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2022424380A1 (en) * 2021-12-29 2024-01-18 Illumina, Inc. Methods of nucleic acid sequencing using surface-bound primers
WO2023187061A1 (en) * 2022-03-31 2023-10-05 Illumina Cambridge Limited Paired-end re-synthesis using blocked p5 primers

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US6537764B1 (en) 1995-01-19 2003-03-25 Children's Medical Center Corporation Method of identifying inhibitors of C—C chemokine receptor 3
JP2001517948A (ja) 1997-04-01 2001-10-09 グラクソ、グループ、リミテッド 核酸配列決定法
AU6846698A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid amplification
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
WO2001079553A1 (en) 2000-04-14 2001-10-25 Lynx Therapeutics, Inc. Method and compositions for ordering restriction fragments
NZ523831A (en) 2000-07-13 2005-10-28 Invitrogen Corp Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix
JP2005518811A (ja) 2002-03-05 2005-06-30 ソレックサ リミテッド 表現型に関連するゲノム全域での配列変化を決定するための方法
SI3363809T1 (sl) 2002-08-23 2020-08-31 Illumina Cambridge Limited Modificirani nukleotidi za polinukleotidno sekvenciranje
EP1682680B2 (en) 2003-10-31 2018-03-21 AB Advanced Genetic Analysis Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
ES2404311T3 (es) 2005-04-12 2013-05-27 454 Life Sciences Corporation Métodos para determinar variantes de secuencias usando secuenciación ultraprofunda
US8428882B2 (en) 2005-06-14 2013-04-23 Agency For Science, Technology And Research Method of processing and/or genome mapping of diTag sequences
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
CA2641851A1 (en) 2006-02-08 2007-08-16 Eric Hans Vermaas Method for sequencing a polynucleotide template
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
US7754429B2 (en) * 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
EP2121983A2 (en) * 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
SI2669387T1 (sl) * 2009-08-25 2017-01-31 Illumina, Inc. Metode za izbiro in pomnoževanje polinukleotidov
EP2470699B1 (en) * 2009-08-27 2017-10-04 LG Electronics Inc. Control method of laundry machine
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
CN102344967B (zh) * 2011-11-02 2014-10-08 东南大学 一种缩短dna模板的dna测序方法及其应用
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
GB201410420D0 (en) * 2014-06-11 2014-07-23 Illumina Cambridge Ltd Methods for estimating cluster numbers
CN107532219B (zh) * 2015-03-25 2023-05-02 安戈欧洲有限公司 使用链置换聚合酶的固相靶核酸捕获和复制
GB201611469D0 (en) * 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes

Also Published As

Publication number Publication date
EP3969618B1 (en) 2023-09-13
DK3969618T3 (da) 2023-10-16
KR20220151101A (ko) 2022-11-14
CA3145445A1 (en) 2021-09-16
AU2021234084A1 (en) 2022-01-06
PL3969618T3 (pl) 2024-01-03
CN114040985A (zh) 2022-02-11
US20220316003A1 (en) 2022-10-06
JP2023517157A (ja) 2023-04-24
FI3969618T3 (fi) 2023-10-18
EP3969618A1 (en) 2022-03-23
ES2965266T3 (es) 2024-04-11
WO2021180733A1 (en) 2021-09-16

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