CN113880660A - Biological organic fertilizer for killing underground insect pests by using biological agent and preparation method thereof - Google Patents

Biological organic fertilizer for killing underground insect pests by using biological agent and preparation method thereof Download PDF

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Publication number
CN113880660A
CN113880660A CN202111094133.6A CN202111094133A CN113880660A CN 113880660 A CN113880660 A CN 113880660A CN 202111094133 A CN202111094133 A CN 202111094133A CN 113880660 A CN113880660 A CN 113880660A
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parts
biological
beauveria bassiana
organic fertilizer
insect pests
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赵化极
赵守忠
曾庆岚
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Hubei Wenxin Agricultural Technology Co ltd
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Hubei Wenxin Agricultural Technology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Agronomy & Crop Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Fertilizers (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a biological organic fertilizer for killing underground insect pests by using a biological agent and a preparation method thereof, wherein the biological organic fertilizer comprises the following raw materials in parts by weight: 5-15 parts of beauveria bassiana microbial inoculum, 10-20 parts of cow dung, 20-30 parts of pig dung, 10-15 parts of chicken manure, 10-15 parts of cassava residue, 5-10 parts of furfural residue and 15-25 parts of sesame cake; the beauveria bassiana microbial inoculum comprises 40-50 parts by weight of beauveria bassiana spore powder mother medicine, 20-30 parts by weight of corn flour and 10-20 parts by weight of glucose powder. The preparation method comprises the following steps: (1) preparing a base fertilizer; (2) culturing a microbial inoculum; (3) airing the base fertilizer; (4) and (4) mixing the microbial inoculum. The organic fertilizer provided by the invention utilizes the biological bactericide to kill underground insect pests, has long lasting period for preventing and controlling the pests, no toxicity to people, bees, fishes and the like, no pollution to the environment, good deinsectization effect and long fertilizer efficiency, and can reduce the environmental pollution caused by deinsectization by chemical pesticides.

Description

Biological organic fertilizer for killing underground insect pests by using biological agent and preparation method thereof
Technical Field
The invention relates to the field of organic fertilizers, in particular to a biological organic fertilizer for killing underground insect pests by using a biological agent and a preparation method thereof.
Background
Subterranean pests refer to omnivorous insects that live in soil for life or at some stage of life and that harm the subterranean parts, seeds, seedlings or near-soil surface stems of plants. The species of the disease varies from place to place, and generally occurs in dry farming areas, particularly grubs, wireworms, cutworms, maggots and nematodes are the most serious. After crops and the like are victimized, light plants will wither, grow slowly, and heavy plants will wither and die, resulting in seedling shortage and ridge breaking, and thus reducing yield.
The pesticide is used for killing insects, and has the following problems: 1. the resistance of the insect pests to the pesticide is enhanced, so that the control effect is greatly reduced; 2. chemical pesticide is difficult to displace in soil, so that the prevention and control effect is increasingly poor, and 3, the duration of pest prevention and control is short.
Disclosure of Invention
The invention aims to solve the problems and provides a biological organic fertilizer for killing underground insect pests by using a biological bactericide and a preparation method thereof, wherein the biological organic fertilizer has the advantages of long pest control duration, no toxicity to people, bees, fishes and the like, no pollution to the environment, good pest killing effect and long fertilizer efficiency.
In order to achieve the purpose of the invention, the invention adopts the following technical scheme.
A biological organic fertilizer for killing underground insect pests by using a biological agent is characterized by comprising the following raw materials in parts by weight: 5-15 parts of beauveria bassiana microbial inoculum, 10-20 parts of cow dung, 20-30 parts of pig dung, 10-15 parts of chicken manure, 10-15 parts of cassava residue, 5-10 parts of furfural residue and 15-25 parts of sesame cake; the beauveria bassiana microbial inoculum comprises 40-50 parts of beauveria bassiana spore powder mother medicine, 20-30 parts of corn flour and 10-20 parts of glucose powder in parts by weight.
Further, the biological organic fertilizer for killing underground insect pests by using the biological bactericide comprises 1.0-1.5 parts by weight of 60-120-mesh organic selenium.
Further, the biological organic fertilizer for killing underground insect pests by using the biological agent is 10-12 parts by weight of the beauveria bassiana microbial agent.
Further, according to the biological organic fertilizer for killing underground insect pests by using the biological bactericide, the spore content of the beauveria bassiana spore powder mother medicine and the beauveria bassiana spore powder mother medicine is more than 500 hundred million/g.
A preparation method of a biological organic fertilizer for killing underground insect pests by using a biological agent comprises the following steps:
(1) preparing a base fertilizer: crushing cassava residues, furfural residues and sesame cakes, mixing the crushed cassava residues, furfural residues and sesame cakes with cow dung, pig dung and chicken dung, feeding the mixture into a fermentation tank, adding a proper amount of water, uniformly mixing the mixture by using a mixing machine, sealing and fermenting, controlling the temperature to be between 50 and 70 ℃ during fermentation, opening a sealing device to dissipate heat when the temperature is too high, and removing the sealing device after fermenting for 12 to 15 days;
(2) and (3) culturing a microbial inoculum: putting the beauveria bassiana spore powder mother medicine and the beauveria bassiana spore powder mother medicine into the corn powder and the glucose powder, mixing and stirring, and culturing for 5-10 days at 25-30 ℃;
(3) and (3) airing the base fertilizer: taking the fermented product obtained in the step (1) out of the pool and airing;
(4) and (3) mixing the microbial inoculum: and (3) uniformly mixing the beauveria bassiana preparation cultured in the step (2), the beauveria bassiana preparation and the base fertilizer aired in the step (3) to obtain the biological organic fertilizer for killing underground insect pests by using the biological agent.
Further, airing in the step (3) until the water content is less than or equal to 10 percent.
Further, adding 60-120 meshes of organic selenium, a beauveria bassiana agent, a beauveria bassiana microbial agent and the aired base fertilizer into the step (4) and uniformly mixing the mixture.
Further, the mixing temperature in the step (4) is controlled to be 25-35 ℃.
The invention has the beneficial effects that: (1) the organic fertilizer provided by the invention utilizes the biological bactericide to kill underground insect pests, has long duration of pest control, is nontoxic to people, bees, fishes and the like, has no pollution to the environment, has the advantages of good pest control effect and long fertilizer efficiency, and can reduce the environmental pollution caused by chemical pesticide pest control. (2) Organic selenium is added into the organic fertilizer, so that the aim of selenium-containing of some fruit trees or vegetables can be fulfilled. (3) The preparation method can achieve the characteristics of high spore content, strong activity and good insecticidal effect of the beauveria bassiana in the final organic fertilizer by controlling the temperature of the culture of the microbial inoculum and the mixing of the microbial inoculum.
Detailed Description
The technical solutions of the present invention are described in detail below with reference to examples so that the advantages and features of the present invention can be more easily understood by those skilled in the art, and thus the scope of the present invention can be more clearly defined.
Example 1:
a biological organic fertilizer for killing underground insect pests by using a biological agent is composed of the following raw materials in parts by weight: 5 parts of beauveria bassiana microbial inoculum, 10 parts of cow dung, 20 parts of pig dung, 10 parts of chicken manure, 10 parts of cassava residue, 5 parts of furfural residue, 15 parts of sesame cake and 1 part of 60-120-mesh organic selenium; the beauveria bassiana microbial inoculum comprises 40 parts by weight of beauveria bassiana spore powder mother medicine, 20 parts by weight of corn flour and 10 parts by weight of glucose powder. The spore contents of the beauveria bassiana spore powder mother medicine and the beauveria bassiana spore powder mother medicine are both more than 500 hundred million/g.
A preparation method of a biological organic fertilizer for killing underground insect pests by using a biological agent comprises the following steps:
(1) preparing a base fertilizer: crushing cassava residue, furfural residue and sesame cake, mixing with cow dung, pig dung and chicken manure, feeding into a fermentation tank, adding a proper amount of water, uniformly mixing by using a mixing machine, sealing and fermenting, controlling the temperature to be between 50 and 60 ℃ during fermentation, opening a sealing device to dissipate heat when the temperature is too high, and removing the sealing device after fermenting for 12 days;
(2) and (3) culturing a microbial inoculum: mixing the mother medicine of Beauveria bassiana spore powder and the mother medicine of Beauveria bassiana spore powder in corn powder and glucose powder, stirring, and culturing at 25 deg.C for 5 days;
(3) and (3) airing the base fertilizer: taking the fermented product in the step (1) out of the pool, airing until the water content is less than or equal to 10%;
(4) and (3) mixing the microbial inoculum: and (3) uniformly mixing the beauveria bassiana preparation cultured in the step (2), the beauveria bassiana preparation, the aired base fertilizer obtained in the step (3) and 60-120-mesh organic selenium to obtain the biological organic fertilizer for killing underground insect pests by using the biological agent.
Example 2:
a biological organic fertilizer for killing underground insect pests by using a biological agent is composed of the following raw materials in parts by weight: 10 parts of beauveria bassiana microbial inoculum, 15 parts of cow dung, 25 parts of pig dung, 12.5 parts of chicken manure, 12.5 parts of cassava residue, 7.5 parts of furfural residue and 20 parts of sesame cake; the beauveria bassiana microbial inoculum comprises 45 parts of beauveria bassiana spore powder mother medicine, 25 parts of corn flour and 15 parts of glucose powder by weight. The spore contents of the beauveria bassiana spore powder mother medicine and the beauveria bassiana spore powder mother medicine are both more than 500 hundred million/g.
A preparation method of a biological organic fertilizer for killing underground insect pests by using a biological agent comprises the following steps:
(1) preparing a base fertilizer: crushing cassava residues, furfural residues and sesame cakes, mixing the crushed cassava residues, furfural residues and sesame cakes with cow dung, pig dung and chicken dung, feeding the mixture into a fermentation tank, adding a proper amount of water, uniformly mixing the mixture by using a mixing machine, sealing and fermenting, controlling the temperature to be between 55 and 65 ℃ during fermentation, opening a sealing device to dissipate heat when the temperature is too high, and removing the sealing device after fermenting for 13 days;
(2) and (3) culturing a microbial inoculum: mixing the mother medicine of Beauveria bassiana spore powder and the mother medicine of Beauveria bassiana spore powder in corn powder and glucose powder, stirring, and culturing at 27.5 deg.C for 7.5 days;
(3) and (3) airing the base fertilizer: taking the fermented product in the step (1) out of the pool, airing until the water content is less than or equal to 10%;
(4) and (3) mixing the microbial inoculum: and (3) uniformly mixing the beauveria bassiana preparation cultured in the step (2), the beauveria bassiana preparation and the base fertilizer aired in the step (3) to obtain the biological organic fertilizer for killing underground insect pests by using the biological agent.
Example 3:
a biological organic fertilizer for killing underground insect pests by using a biological agent is composed of the following raw materials in parts by weight: 15 parts of beauveria bassiana microbial inoculum, 20 parts of cow dung, 30 parts of pig dung, 15 parts of chicken manure, 15 parts of cassava residue, 10 parts of furfural residue, 25 parts of sesame cake and 1.5 parts of 60-120-mesh organic selenium; the beauveria bassiana microbial inoculum comprises 50 parts of beauveria bassiana spore powder mother medicine, 30 parts of corn flour and 20 parts of glucose powder by weight. The spore contents of the beauveria bassiana spore powder mother medicine and the beauveria bassiana spore powder mother medicine are both more than 500 hundred million/g.
A preparation method of a biological organic fertilizer for killing underground insect pests by using a biological agent is characterized by comprising the following steps:
(1) preparing a base fertilizer: crushing cassava residues, furfural residues and sesame cakes, mixing the crushed cassava residues, furfural residues and sesame cakes with cow dung, pig dung and chicken dung, feeding the mixture into a fermentation tank, adding a proper amount of water, uniformly mixing the mixture by using a mixing machine, sealing and fermenting, controlling the temperature to be between 60 and 70 ℃ during fermentation, opening a sealing device to dissipate heat when the temperature is too high, and removing the sealing device after fermenting for 15 days;
(2) and (3) culturing a microbial inoculum: mixing the mother medicine of Beauveria bassiana spore powder and the mother medicine of Beauveria bassiana spore powder in corn powder and glucose powder, stirring, and culturing at 30 deg.C for 10 days;
(3) and (3) airing the base fertilizer: taking the fermented product in the step (1) out of the pool, airing until the water content is less than or equal to 10%;
(4) and (3) mixing the microbial inoculum: and (3) uniformly mixing the beauveria bassiana preparation cultured in the step (2), the beauveria bassiana preparation, the aired base fertilizer obtained in the step (3) and 60-120-mesh organic selenium to obtain the biological organic fertilizer for killing underground insect pests by using the biological bactericide.
In the above embodiment, by adding a proper amount of beauveria bassiana microbial inoculum in the basic organic fertilizer, fermenting the basic organic fertilizer firstly, culturing the beauveria bassiana microbial inoculum separately, and controlling the temperature for mixing the microbial inoculum, the finally prepared organic fertilizer has high spore content of the beauveria bassiana microbial inoculum, strong activity, good insecticidal effect and long insecticidal time, can completely replace pesticides for insecticidal, and reduces the pollution of the pesticides to soil and surrounding environment.
The foregoing is merely illustrative of the preferred embodiments of the present invention and is not to be construed as limiting the claims. The present invention is not limited to the above embodiments, and the specific structure thereof is allowed to vary. All changes which come within the scope of the invention as defined by the independent claims are intended to be embraced therein.

Claims (8)

1. A biological organic fertilizer for killing underground insect pests by using a biological agent is characterized by comprising the following raw materials in parts by weight: 5-15 parts of beauveria bassiana microbial inoculum, 10-20 parts of cow dung, 20-30 parts of pig dung, 10-15 parts of chicken manure, 10-15 parts of cassava residue, 5-10 parts of furfural residue and 15-25 parts of sesame cake; the beauveria bassiana microbial inoculum comprises 40-50 parts of beauveria bassiana spore powder mother medicine, 20-30 parts of corn flour and 10-20 parts of glucose powder in parts by weight.
2. The biological organic fertilizer for killing underground pests by using the biological bactericide as claimed in claim 1, wherein the raw material composition further comprises 1.0-1.5 parts by weight of 60-120 mesh organic selenium.
3. The biological organic fertilizer for killing underground pests by using the biological bactericide as claimed in claim 1, wherein the weight part of the beauveria bassiana bactericide is 10-12.
4. The bio-organic fertilizer for killing underground insect pests by using the biological agent as claimed in claim 1, wherein the spore contents of the beauveria bassiana spore powder mother medicine and the beauveria bassiana spore powder mother medicine are all more than 500 hundred million/g.
5. A preparation method of a biological organic fertilizer for killing underground insect pests by using a biological agent is characterized by comprising the following steps:
(1) preparing a base fertilizer: crushing cassava residues, furfural residues and sesame cakes, mixing the crushed cassava residues, furfural residues and sesame cakes with cow dung, pig dung and chicken dung, feeding the mixture into a fermentation tank, adding a proper amount of water, uniformly mixing the mixture by using a mixing machine, sealing and fermenting, controlling the temperature to be between 50 and 70 ℃ during fermentation, opening a sealing device to dissipate heat when the temperature is too high, and removing the sealing device after fermenting for 12 to 15 days;
(2) and (3) culturing a microbial inoculum: putting the beauveria bassiana spore powder mother medicine and the beauveria bassiana spore powder mother medicine into the corn powder and the glucose powder, mixing and stirring, and culturing for 5-10 days at 25-30 ℃;
(3) and (3) airing the base fertilizer: taking the fermented product obtained in the step (1) out of the pool and airing;
(4) and (3) mixing the microbial inoculum: and (3) uniformly mixing the beauveria bassiana preparation cultured in the step (2), the beauveria bassiana preparation and the base fertilizer aired in the step (3) to obtain the biological organic fertilizer for killing underground insect pests by using the biological agent.
6. The preparation method of the biological organic fertilizer for killing underground insect pests by using the biological agent as claimed in claim 5, is characterized in that: and (4) airing until the water content is less than or equal to 10 percent.
7. The preparation method of the biological organic fertilizer for killing underground insect pests by using the biological agent as claimed in claim 5, is characterized in that: and (4) adding 60-120 meshes of organic selenium, a beauveria bassiana agent, a Beauveria bassiana microbial inoculum and a base fertilizer after airing, and uniformly mixing.
8. The preparation method of the biological organic fertilizer for killing underground insect pests by using the biological bactericide as claimed in claim 5 or 7, is characterized in that: in the step (4), the mixing temperature is controlled to be 25-35 ℃.
CN202111094133.6A 2021-09-17 2021-09-17 Biological organic fertilizer for killing underground insect pests by using biological agent and preparation method thereof Pending CN113880660A (en)

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN114467977A (en) * 2022-01-26 2022-05-13 春华秋实科技集团有限公司 Insecticidal composite microbial composition suitable for drip irrigation facilities and application thereof

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Application publication date: 20220104