CN113827641A - Traditional Chinese medicine immunomodulator, preparation method and application - Google Patents
Traditional Chinese medicine immunomodulator, preparation method and application Download PDFInfo
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- CN113827641A CN113827641A CN202111126312.3A CN202111126312A CN113827641A CN 113827641 A CN113827641 A CN 113827641A CN 202111126312 A CN202111126312 A CN 202111126312A CN 113827641 A CN113827641 A CN 113827641A
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Abstract
The invention provides a traditional Chinese medicine immunomodulator, a preparation method and application thereof, wherein the preparation raw materials comprise solid materials and fermentation probiotics; the solid material comprises: 35 to 52 parts of middle-jiao and qi tonifying powder, 10 to 50 parts of rice flour and 10 to 50 parts of cooking range soil; the fermentation probiotics comprise one or more of enterococcus faecalis, bacillus licheniformis, saccharomycetes, bacillus subtilis or lactobacillus; wherein, each gram of solid material is fermented by 0.03ml to 0.05ml of fermentation probiotics to obtain the traditional Chinese medicine immunomodulator. Compared with the prior art, the invention has the beneficial effects that: the invention adds the powder for tonifying middle-jiao and Qi into rice flour and stove top soil to ferment by adopting probiotics, and can obviously improve the immunity of a subject to take.
Description
Technical Field
The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine immunomodulator, a preparation method and application thereof.
Background
The middle-jiao-tonifying and qi-benefiting powder is a common veterinary drug taking radix astragali, radix codonopsis, rhizoma atractylodis macrocephalae, liquorice, angelica, dried orange peel, rhizoma cimicifugae and bupleurum root as a formula. It can be used for treating spleen and stomach deficiency, chronic diarrhea, proctoptosis and uterine prolapse. Has good effects on reducing appetite of livestock, and relieving lassitude. According to the traditional Chinese medicine, the livestock has the diseases mainly caused by the chronic deficiency of the spleen and the stomach, and the chronic deficiency of the spleen and the stomach can cause the insufficient immunity, so that the middle-jiao-tonifying and qi-tonifying powder also has the effect of improving the immunity.
Nowadays, with the abuse of antibiotics and the increasing serious problem of drug residues in animal products, the food safety problem is receiving more and more attention. The traditional Chinese medicine is the focus of research by virtue of safety, greenness and no residue, but compared with western medicines such as antibiotics and the like, the traditional Chinese medicine has the problem of difficult absorption. Similarly, the effect of improving immunity is affected by the qi-tonifying powder which is not easy to absorb.
Disclosure of Invention
The invention provides a traditional Chinese medicine immunomodulator, a preparation method and application in order to solve the technical problem that the effect of strengthening the middle-jiao and replenishing qi in improving immunity is not good in the prior art.
The specific technical scheme is as follows:
a traditional Chinese medicine immunomodulator is characterized in that raw materials for preparing the immunomodulator comprise solid materials and fermented probiotics; the solid material comprises: 35 to 52 parts of middle-jiao and qi tonifying powder, 10 to 50 parts of rice flour and 10 to 50 parts of cooking range soil; the fermentation probiotics comprise one or more of enterococcus faecalis, bacillus licheniformis, saccharomycetes, bacillus subtilis or lactobacillus; wherein, each gram of solid material is fermented by 0.03ml to 0.05ml of fermentation probiotics to obtain the traditional Chinese medicine immunomodulator.
Furthermore, the volume percentage of the enterococcus faecalis is 2 to 30 percent, the volume percentage of the bacillus licheniformis is 8 to 45 percent and the volume percentage of the yeast is 30 to 65 percent.
Further, the fermentation probiotics comprise the following components in percentage by weight: 3 to 8 percent of enterococcus faecalis, 8 to 15 percent of bacillus licheniformis, 30 to 35 percent of saccharomycete, 15 to 40 percent of bacillus subtilis and 5 to 30 percent of lactobacillus.
The preparation method of the traditional Chinese medicine immunomodulator is characterized by comprising the following steps:
mixing the middle-jiao-tonifying and qi-benefiting powder, rice flour and cooking range soil, adding water and the fermentation probiotics, and fermenting to obtain the health-care food.
Further, the middle-jiao and qi tonifying powder, the rice flour and the range soil are mixed, water with the mass ratio of 25-35% is added, and the fermentation probiotics are added for fermentation.
Furthermore, the particle sizes of the middle-jiao tonifying and qi benefiting powder, the rice flour and the range soil are 60-80 meshes.
Furthermore, the fermentation temperature is 35-40 ℃, the humidity is 30-70%, and the fermentation time is 3-7 days.
The application of the traditional Chinese medicine immunomodulator in preparing medicines or foods for improving immunity.
Further, the application of the traditional Chinese medicine immunomodulator in preparing medicines or foods for improving the immunity of livestock is provided.
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention adds the powder for tonifying middle-jiao and Qi into rice flour and stove top soil to ferment by adopting probiotics, and can obviously improve the immunity of a subject to take.
(2) When enterococcus faecalis, bacillus licheniformis and saccharomycetes mixed probiotics are adopted for fermentation, the effect of improving the immunity is further enhanced.
(3) When enterococcus faecalis, bacillus licheniformis, saccharomycetes, bacillus subtilis or lactobacillus are mixed and fermented, the IgG value of the mixed fermentation product reaches 3.84, and the IL-6 value of the mixed fermentation product reaches 25.13.
(4) The particle sizes of the middle-jiao tonifying and qi benefiting powder, the rice flour and the cooking range soil are controlled to be 60-80 meshes, so that complete fermentation can be further ensured.
Detailed Description
The present invention is further described in detail below with reference to specific examples so that those skilled in the art can more clearly understand the present invention.
The following examples are provided only for illustrating the present invention and are not intended to limit the scope of the present invention. All other embodiments obtained by a person skilled in the art based on the specific embodiments of the present invention without any inventive step are within the scope of the present invention.
In the examples of the present invention, all the raw material components are commercially available products well known to those skilled in the art, unless otherwise specified; in the examples of the present invention, unless otherwise specified, all technical means used are conventional means well known to those skilled in the art.
In the examples of the present invention, the raw materials used were all conventional commercially available products.
The invention relates to the following raw material descriptions:
powder for tonifying middle-jiao and qi: produced by Wuhan Huayang animal Limited liability company (available on the market, available for inquiry), and has a specification of 500 g/bag.
Stove core soil: purchased from Tongrentang, Beijing.
Enterococcus faecalis: the number of viable bacteria is 200 hundred million cfu/ml.
B, bacillus licheniformis: the number of viable bacteria is 200 hundred million cfu/ml.
Yeast: the number of viable bacteria is 200 hundred million cfu/ml.
B, bacillus subtilis: the number of viable bacteria is 200 hundred million cfu/ml.
Lactobacillus plantarum: the number of viable bacteria is 200 hundred million cfu/ml.
Example 1
The embodiment provides a traditional Chinese medicine immunomodulator, which comprises the following raw materials:
powder for tonifying middle-jiao and qi: 50 parts of rice flour: 20 parts of stove core soil: 30 parts of yeast and 5ml of yeast are adopted for fermenting per 100g of solid material.
The embodiment also provides a preparation method of the traditional Chinese medicine immunomodulator, which comprises the following specific steps:
(1) dry mixing: sieving the middle-jiao and qi tonifying powder and the rice flour by 60-80 meshes, accurately weighing the required raw materials according to the specified proportion, putting the raw materials into a mixing tank, dry-mixing for 5-10min, and uniformly stirring.
(2) Preparing liquid: adding purified water with the formula amount to ensure that the water content reaches 30% into a material mixing tank, adding yeast, and uniformly stirring for later use.
(3) Wet mixing: and (3) putting the bacterial liquid in the step (2) into a mixing tank, wet-mixing for 5-10min, uniformly stirring, and putting into a fermentation bag.
(4) Fermentation: the temperature of the packed fermentation material in a fermentation chamber is 37 ℃; continuously fermenting for 5 d; and (5) obtaining the product.
Example 2
The embodiment provides a traditional Chinese medicine immunomodulator, which comprises the following raw materials:
powder for tonifying middle-jiao and qi: 45 parts of rice flour: 25 parts of stove core soil: 30 parts of solid material, and fermenting by adopting 5ml of mixed strains per 100g of solid material, wherein the mixed strains comprise 20% of enterococcus faecalis, 20% of bacillus licheniformis and 60% of yeast in percentage by volume.
The embodiment also provides a preparation method of the traditional Chinese medicine immunomodulator, which comprises the following specific steps:
(1) dry mixing: sieving the middle-jiao and qi tonifying powder and the rice flour by 60-80 meshes, accurately weighing the required raw materials according to the specified proportion, putting the raw materials into a mixing tank, dry-mixing for 5-10min, and uniformly stirring.
(2) Preparing liquid: adding purified water with the formula amount to ensure that the water content reaches 30% into a material mixing tank, adding mixed strains containing saccharomycetes, enterococcus faecalis and bacillus licheniformis, and uniformly stirring for later use.
(3) Wet mixing: and (3) putting the bacterial liquid in the step (2) into a mixing tank, wet-mixing for 5-10min, uniformly stirring, and putting into a fermentation bag.
(4) Fermentation: the temperature of the packed fermentation material in a fermentation chamber is 37 ℃; continuously fermenting for 5 d; and (5) obtaining the product.
Example 3
The embodiment provides a traditional Chinese medicine immunomodulator, which comprises the following raw materials:
powder for tonifying middle-jiao and qi: 45 parts of rice flour: 25 parts of stove core soil: 30 parts of solid material, and fermenting by adopting 3ml of mixed strains per 100g of solid material, wherein the mixed strains comprise 20% of enterococcus faecalis, 20% of bacillus licheniformis and 60% of yeast in percentage by volume.
The embodiment also provides a preparation method of the traditional Chinese medicine immunomodulator, which comprises the following specific steps:
(1) dry mixing: sieving the middle-jiao and qi tonifying powder and the rice flour with 60-80 meshes, accurately weighing the required raw materials according to the specified proportion, putting the raw materials into a mixing tank, dry-mixing for 5-10min, and uniformly stirring.
(2) Preparing liquid: adding purified water with the formula amount to ensure that the water content reaches 30% into a material mixing tank, adding mixed strains containing saccharomycetes, enterococcus faecalis and bacillus licheniformis, and uniformly stirring for later use.
(3) Wet mixing: and (3) putting the bacterial liquid in the step (2) into a mixing tank, wet-mixing for 5-10min, uniformly stirring, and putting into a fermentation bag.
(4) Fermentation: the temperature of the packed fermentation material in a fermentation chamber is 37 ℃; fermenting for 5 days to obtain the final product.
Examples 4 to 6
Embodiments 4 to 6 provide a traditional Chinese medicine immunomodulator, which is prepared from the following raw materials:
powder for tonifying middle-jiao and qi: 45 parts of rice flour: 25 parts of stove core soil: 30 parts of the mixed strain is fermented by 5ml of the mixed strain per 100g of solid material, and the types and volume percentages of the mixed strain strains of the examples 4 to 6 are shown in the table 1.
Embodiments 4 to 6 further provide a method for preparing a traditional Chinese medicine immunomodulator, which comprises the following specific steps:
(1) dry mixing: sieving the middle-jiao and qi tonifying powder and the rice flour with 60-80 meshes, accurately weighing the required raw materials according to the specified proportion, putting the raw materials into a mixing tank, dry-mixing for 5-10min, and uniformly stirring.
(2) Preparing liquid: adding purified water to make water content reach 30%, adding yeast, enterococcus faecalis, Bacillus licheniformis, Bacillus subtilis and Lactobacillus, and stirring.
(3) Wet mixing: and (3) putting the bacterial liquid in the step (2) into a mixing tank, wet-mixing for 5-10min, uniformly stirring, and putting into a fermentation bag.
(4) Fermentation: the temperature of the packed fermentation material in a fermentation chamber is 37 ℃; fermenting for 5 days to obtain the final product.
TABLE 1 species of mixed species and volume percentages of examples 4-6
| Example 4 (% v/v) | Example 5 (% v/v) | Example 6 (% v/v) | |
| Enterococcus faecalis | 5 | 5 | 3 |
| Bacillus licheniformis | 5 | 10 | 15 |
| Yeast | 30 | 35 | 32 |
| Bacillus subtilis | 30 | 35 | 30 |
| Lactobacillus lactis | 30 | 15 | 20 |
Examples 7 to 9
Embodiments 7 to 9 provide a traditional Chinese medicine immunomodulator, which is prepared from the following raw materials:
middle-jiao and qi tonifying powder, rice flour and cooking range soil; every 100g of solid material is fermented by adopting 5ml of mixed strains, the mixed strains comprise 5% of saccharomycetes, 5% of enterococcus faecalis, 30% of bacillus licheniformis, 30% of bacillus subtilis and 30% of lactobacillus by volume percentage, and the solid material content of the embodiments 7-9 is shown in the table 2.
TABLE 2 EXAMPLES 7 TO 9 SOLID MATERIAL QUALITY PARTS
Embodiments 7 to 9 further provide a method for preparing a traditional Chinese medicine immunomodulator, which comprises the following specific steps:
(1) dry mixing: sieving the middle-jiao and qi tonifying powder and the rice flour with 60-80 meshes, accurately weighing the required raw materials according to the specified proportion, putting the raw materials into a mixing tank, dry-mixing for 5-10min, and uniformly stirring.
(2) Preparing liquid: adding purified water to make water content reach 30%, adding yeast, enterococcus faecalis, Bacillus licheniformis, Bacillus subtilis and Lactobacillus, and stirring.
(3) Wet mixing: and (3) putting the bacterial liquid in the step (2) into a mixing tank, wet-mixing for 5-10min, uniformly stirring, and putting into a fermentation bag.
(4) Fermentation: the temperature of the packed fermentation material in a fermentation chamber is 37 ℃; fermenting for 5 days to obtain the final product.
Example 10
The embodiment provides a traditional Chinese medicine immunomodulator, which comprises the following raw materials:
powder for tonifying middle-jiao and qi: 45 parts of rice flour: 25 parts of stove core soil: 30 parts of mixed strains are adopted for fermenting every 100g of solid material, and the mixed strains comprise 20 percent of enterococcus faecalis, 20 percent of lactobacillus and 60 percent of yeast by volume percentage.
The embodiment also provides a preparation method of the traditional Chinese medicine immunomodulator, which comprises the following specific steps:
(1) dry mixing: sieving the middle-jiao and qi tonifying powder and the rice flour by 60-80 meshes, accurately weighing the required raw materials according to the specified proportion, putting the raw materials into a mixing tank, dry-mixing for 5-10min, and uniformly stirring.
(2) Preparing liquid: adding purified water with the formula amount to ensure that the water content reaches 30% into a material mixing tank, adding mixed strains containing saccharomycetes, enterococcus faecalis and lactobacillus, and uniformly stirring for later use.
(3) Wet mixing: and (3) putting the bacterial liquid in the step (2) into a mixing tank, wet-mixing for 5-10min, uniformly stirring, and putting into a fermentation bag.
(4) Fermentation: the temperature of the packed fermentation material in a fermentation chamber is 37 ℃; continuously fermenting for 5 d; and (5) obtaining the product.
Example 11
The embodiment provides a traditional Chinese medicine immunomodulator, which comprises the following raw materials:
powder for tonifying middle-jiao and qi: 45 parts of rice flour: 25 parts of stove core soil: 30 parts of solid material, and fermenting by adopting 5ml of mixed strains per 100g of solid material, wherein the mixed strains comprise 20 percent of bacillus subtilis, 20 percent of lactobacillus cocci and 60 percent of microzyme in percentage by volume.
The embodiment also provides a preparation method of the traditional Chinese medicine immunomodulator, which comprises the following specific steps:
(1) dry mixing: sieving the middle-jiao and qi tonifying powder and the rice flour by 60-80 meshes, accurately weighing the required raw materials according to the specified proportion, putting the raw materials into a mixing tank, dry-mixing for 5-10min, and uniformly stirring.
(2) Preparing liquid: adding purified water with the formula amount to ensure that the water content reaches 30% into a material mixing tank, adding mixed strains containing saccharomycetes, bacillus subtilis and lactobacillus, and uniformly stirring for later use.
(3) Wet mixing: and (3) putting the bacterial liquid in the step (2) into a mixing tank, wet-mixing for 5-10min, uniformly stirring, and putting into a fermentation bag.
(4) Fermentation: the temperature of the packed fermentation material in a fermentation chamber is 37 ℃; continuously fermenting for 5 d; and (5) obtaining the product.
Comparative example 1
The comparative example provides a traditional Chinese medicine immunomodulator, which is prepared from the following raw materials:
powder for tonifying middle-jiao and qi: 45 parts of soybean meal: 25 parts of stove core soil: 30 parts of solid material, and fermenting by adopting 5ml of mixed strains per 100g of solid material, wherein the mixed strains comprise 5% of saccharomycetes, 5% of enterococcus faecalis, 30% of bacillus licheniformis, 30% of bacillus subtilis and 30% of lactobacillus.
The embodiment also provides a preparation method of the traditional Chinese medicine immunomodulator, which comprises the following specific steps:
(1) dry mixing: sieving the middle-jiao and qi tonifying powder and the rice flour by 60-80 meshes, accurately weighing the required raw materials according to the specified proportion, putting the raw materials into a mixing tank, dry-mixing for 5-10min, and uniformly stirring.
(2) Preparing liquid: adding purified water to make water content reach 30%, and adding yeast, enterococcus faecalis, Bacillus licheniformis, Bacillus subtilis and Lactobacillus. Mixing the strains, and stirring well for later use.
(3) Wet mixing: and (3) putting the bacterial liquid in the step (2) into a mixing tank, wet-mixing for 5-10min, uniformly stirring, and putting into a fermentation bag.
(4) Fermentation: the temperature of the packed fermentation material in a fermentation chamber is 37 ℃; continuously fermenting for 5 d; and (5) obtaining the product.
Comparative example 2
The comparative example provides a traditional Chinese medicine immunomodulator, which is prepared from the following raw materials:
powder for tonifying middle-jiao and qi: 45 parts of rice flour: 25 parts of (1); 5ml of mixed strains are adopted for fermenting every 100g of solid materials, and the mixed strains comprise 5 percent of saccharomycetes, 5 percent of enterococcus faecalis, 30 percent of bacillus licheniformis, 30 percent of bacillus subtilis and 30 percent of lactobacillus.
The embodiment also provides a preparation method of the traditional Chinese medicine immunomodulator, which comprises the following specific steps:
(1) dry mixing: sieving the middle-jiao and qi tonifying powder and the rice flour by 60-80 meshes, accurately weighing the required raw materials according to the specified proportion, putting the raw materials into a mixing tank, dry-mixing for 5-10min, and uniformly stirring.
(2) Preparing liquid: adding purified water to make water content reach 30%, and adding yeast, enterococcus faecalis, Bacillus licheniformis, Bacillus subtilis and Lactobacillus. Mixing the strains, and stirring well for later use.
(3) Wet mixing: and (3) putting the bacterial liquid in the step (2) into a mixing tank, wet-mixing for 5-10min, uniformly stirring, and putting into a fermentation bag.
(4) Fermentation: the temperature of the packed fermentation material in a fermentation chamber is 37 ℃; continuously fermenting for 5 d; and (5) obtaining the product.
Example 12
In the embodiment, the traditional Chinese medicine immunomodulators in the embodiments 1 to 11 and the comparative examples 1 to 2 are selected to feed pigs, and the specific method is as follows: 95 kg-105 kg of breeding pigs (purchased from Wuhan Tianzhong) with the age of 120 days are averagely divided into 13 experimental groups, each group comprises 40 male and female halves.
After the pig is fed, the pig needs to be continuously administrated for 1 month, and the traditional Chinese medicine immunomodulator in each embodiment and the corresponding proportion is doped in the basic feed and accounts for 1 percent of the whole mass of the pig.
After 1 month of drug administration, the pig was sacrificed and immune organ coefficients such as thymus and spleen were calculated, wherein the thymus index is thymus mass (mg)/body mass (g), and the spleen index is spleen mass (mg)/body mass (g).
The details are shown in table 1. As can be seen from the table, the fermented middle-jiao-tonifying qi-tonifying powder has great difference and statistical significance of difference (P <0.05) when compared with the unfermented middle-jiao-tonifying qi-tonifying powder group. The spleen index in the middle-jiao-tonifying qi-tonifying powder fermentation group is different from that in the former two groups, and P is less than 0.05.
TABLE 3 thymus index and spleen index test values for examples and comparative examples
In this example, compared with comparative example 1, the rice flour is used in combination with the middle-jiao qi tonifying powder for fermentation, and compared with comparative example 2, the center-jiao soil is added in combination with the middle-jiao qi tonifying powder for fermentation, and according to in vivo experiments of pig feeding, after the raw material formula disclosed by the invention is used for fermentation, the thymus index and the spleen index of pigs are better than those of unfermented middle-jiao qi tonifying powder, the thymus index reaches more than 4.22, and the spleen index reaches more than 6.1.
Meanwhile, when the composite zymocyte adopts enterococcus faecalis, bacillus licheniformis and saccharomycetes, the thymus index reaches more than 4.4, and the spleen index reaches more than 6.18. Furthermore, when enterococcus faecalis, bacillus licheniformis, saccharomycetes, bacillus subtilis and lactobacillus are used for fermentation, the thymus index reaches more than 5.0, and the spleen index reaches more than 7.
It should be noted that the above examples are only for further illustration and description of the technical solution of the present invention, and are not intended to further limit the technical solution of the present invention, and the method of the present invention is only a preferred embodiment, and is not intended to limit the protection scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A traditional Chinese medicine immunomodulator is characterized in that raw materials for preparing the immunomodulator comprise solid materials and fermented probiotics; the solid material comprises: 35 to 52 parts of middle-jiao and qi tonifying powder, 10 to 50 parts of rice flour and 10 to 50 parts of cooking range soil; the fermentation probiotics comprise one or more of enterococcus faecalis, bacillus licheniformis, saccharomycetes, bacillus subtilis or lactobacillus; wherein, each gram of solid material is fermented by 0.03ml to 0.05ml of fermentation probiotics to obtain the traditional Chinese medicine immunomodulator.
2. The traditional Chinese medicine immunomodulator according to claim 1, wherein the fermented probiotic comprises the following components in content: enterococcus faecalis with the volume percentage of 2-30%, bacillus licheniformis with the volume percentage of 8-45% and saccharomycetes with the volume percentage of 30-65%.
3. The traditional Chinese medicine immunomodulator according to claim 1 or 2, wherein the volume percentage of enterococcus faecalis is 3-8%, the volume percentage of bacillus licheniformis is 8-15%, the volume percentage of saccharomycetes is 30-35%, the volume percentage of bacillus subtilis is 15-40%, and the volume percentage of lactobacillus is 5-30%.
4. The preparation method of the traditional Chinese medicine immunomodulator according to the claims 1-3 is characterized by comprising the following steps:
mixing the middle-jiao-tonifying and qi-benefiting powder, rice flour and cooking range soil, adding water and the fermentation probiotics, and fermenting to obtain the health-care food.
5. The preparation method of the traditional Chinese medicine immunomodulator according to claim 4, wherein the powder for tonifying middle-jiao and qi, the rice flour and the furnace soil are mixed, water with the mass ratio of 25% -35% is added, and then the fermented probiotics are added for fermentation.
6. The method for preparing a traditional Chinese medicine immunomodulator according to claim 4 or 5, wherein the particle sizes of the powder for invigorating middle-jiao and replenishing qi, the rice flour and the hearth soil are 60-80 meshes.
7. The method for preparing a Chinese medicinal immunomodulator according to claim 5, wherein the fermentation temperature is 35-40 ℃, the humidity is 30-70%, and the fermentation time is 3-7 days.
8. Use of the traditional Chinese medicine immunomodulator according to any one of claims 1 to 3 in preparation of drugs or foods for improving immunity.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204121449U (en) * | 2014-08-25 | 2015-01-28 | 河南众翔百成兽药有限公司 | Utilize the equipment of the scattered producing microbial Chinese medicine of composite bacteria fermentation invigorating the spleen and replenishing QI |
| CN105876208A (en) * | 2016-04-29 | 2016-08-24 | 宿州天雨养殖（集团）有限公司 | Appetite-promoting spleen-tonifying pig feed and preparation method thereof |
| CN105985916A (en) * | 2015-01-28 | 2016-10-05 | 河南惠通天下动物药业有限公司 | Composite microorganism preparation and preparation method thereof |
| CN106619941A (en) * | 2017-02-27 | 2017-05-10 | 哈尔滨中科生物工程有限公司 | Traditional Chinese medicine fermented preparation for improving production performance of sows, preparation method and application thereof |
-
2021
- 2021-09-24 CN CN202111126312.3A patent/CN113827641A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204121449U (en) * | 2014-08-25 | 2015-01-28 | 河南众翔百成兽药有限公司 | Utilize the equipment of the scattered producing microbial Chinese medicine of composite bacteria fermentation invigorating the spleen and replenishing QI |
| CN105985916A (en) * | 2015-01-28 | 2016-10-05 | 河南惠通天下动物药业有限公司 | Composite microorganism preparation and preparation method thereof |
| CN105876208A (en) * | 2016-04-29 | 2016-08-24 | 宿州天雨养殖（集团）有限公司 | Appetite-promoting spleen-tonifying pig feed and preparation method thereof |
| CN106619941A (en) * | 2017-02-27 | 2017-05-10 | 哈尔滨中科生物工程有限公司 | Traditional Chinese medicine fermented preparation for improving production performance of sows, preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 李可等: "益生菌对肉仔鸡生产性能、胴体性状、免疫功能和肉品质的影响", 《动物营养学报》 * |
| 沈丕安: "《中药药理与临床运用》", 30 June 2020, 吉林科学技术出版社 * |
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Application publication date: 20211224 |