CN113773138A - Disease-preventing and insect-preventing compound microbial agent combining amino acid and humic acid - Google Patents
Disease-preventing and insect-preventing compound microbial agent combining amino acid and humic acid Download PDFInfo
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- CN113773138A CN113773138A CN202111074060.4A CN202111074060A CN113773138A CN 113773138 A CN113773138 A CN 113773138A CN 202111074060 A CN202111074060 A CN 202111074060A CN 113773138 A CN113773138 A CN 113773138A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a disease-preventing and insect-preventing compound microbial agent combining amino acid and humic acid, which comprises the following components in parts by weight: 20-50 parts of humic acid; 20-50 parts of a microbial agent; 5-10 parts of trace elements; 5-10 parts of alginic acid; 2-4 parts of biological disease-preventing and insect-preventing substances; 5-10 parts of nano silicon; 5-10 parts of citric acid and 100 parts of purified water. The disease-preventing and insect-preventing compound microbial agent combining the amino acid and the humic acid has the advantages of simple production process, no residue or toxic or side effect, capability of regulating the metabolism of crops and stimulating the growth of the crops, capability of increasing the yield of the crops, no pollution, low cost, capability of reducing plant diseases and insect pests and effectively relieving soil hardening, and suitability for large-area popularization and application.
Description
Technical Field
The invention relates to the technical field of agricultural microbial preparations, in particular to a disease-preventing and insect-preventing compound microbial agent combining amino acid and humic acid.
Background
Due to the fact that chemical fertilizers and chemical pesticides are excessively used, land pollution of agriculture and forestry is serious, soil is degraded, the quality of produced agricultural products is reduced, difficulties and obstacles are created for improving the beautiful life of people in a new era, in order to solve the problems of land pollution and degradation, the national government has proposed that zero fertilizer growth is realized from 2020, because the agricultural and forestry production for many years has generated dependence on the use of the chemical fertilizers and the pesticides, if the use of the chemical fertilizers and the pesticides is reduced, the yield of the agricultural products is reduced, in order to reduce the use of the chemical fertilizers and the pesticides and ensure that the agricultural and forestry production is not reduced and increased, the polluted soil needs to be improved and the original soil ecology is recovered, Chinese science and technology personnel carry out a large amount of research and screening, and the use of microbial agents and microbial pesticides in the agricultural and forestry production is an effective method. Although the existing agricultural microbial agent is organic and environment-friendly, the fertilizer efficiency is poor, the effect taking speed is slow after the application, the resistance of crops cannot be enhanced, the number of flowering and granulation cannot be increased, the use effect is low, the production process of the existing agricultural microbial agent is complicated, the production cost is high, the use cost is high, and the economic benefit of farmers is greatly reduced.
Disclosure of Invention
The invention aims to provide the disease-preventing and insect-preventing compound microbial agent combining the amino acid and the humic acid, which has the advantages of simple production process, no residue and toxic or side effect, capability of regulating the metabolism of crops and stimulating the growth of the crops, capability of increasing the yield of the crops, no pollution, low cost, capability of reducing plant diseases and insect pests and effectively relieving soil hardening, and suitability for large-area popularization and application.
In order to achieve the purpose, the invention provides a disease-preventing and insect-preventing compound microbial agent combining amino acid and humic acid, which comprises the following components in parts by weight:
20-50 parts of humic acid; 20-50 parts of a microbial agent; 5-10 parts of trace elements; 5-10 parts of alginic acid; 2-4 parts of biological disease-preventing and insect-preventing substances; 5-10 parts of nano silicon; 5-10 parts of citric acid and 100 parts of purified water; wherein the microbial agent is one or more of trichoderma harzianum, purple spore fungus, beauveria bassiana, bacillus thuringiensis, paecilomyces lilacinus and the like; the biological disease-preventing and insect-preventing substance is one or more of castor leaf powder, peach leaf liquid, mulberry leaf liquid, masson pine liquid, ailanthus leaf liquid, tobacco powder, artemisia fresh grass liquid, Chinese ash liquid, invar leaf liquid, ginkgo leaf liquid and the like.
Preferably, the trace element is at least one of amino acid coupled iron, amino acid coupled zinc, amino acid coupled molybdenum, amino acid coupled copper, amino acid coupled manganese, amino acid coupled boron and amino acid coupled (iron, zinc, molybdenum, copper, manganese and boron).
Preferably, the alginic acid is at least one of potassium alginate, ammonium alginate, sodium alginate and alginic acid essence.
A preparation method of a disease-preventing and insect-preventing compound microbial agent combining amino acid and humic acid comprises the steps of sequentially adding purified water, humic acid, trace elements, alginic acid and nano silicon into a fermentation tank according to the weight part of claim 1 to prepare a mixed solution, adjusting the pH value to 5.5-7 by using citric acid, heating to 35-38 ℃ under stirring, adding the microbial agent and biological disease-preventing and insect-preventing substances, injecting aerobic gas into the bottom layer of the fermentation tank to perform gas stirring and mechanical stirring, and fermenting for 72-96 hours to obtain the disease-preventing and insect-preventing compound microbial agent combining amino acid and humic acid.
Therefore, the disease-preventing and insect-preventing compound microbial agent combining the amino acid and the humic acid has the advantages of simple production process, no residue, no toxic or side effect, reasonable flora compatibility, large quantity of beneficial bacteria, high biological activity, capability of regulating metabolism of crops and stimulating growth of the crops, capability of achieving the effect of increasing yield of the crops, no pollution, low cost and capability of reducing plant diseases and insect pests, and effectively relieving soil hardening due to the matching of the humic acid and trace elements, and is suitable for large-area popularization and application.
The technical solution of the present invention is further described in detail by the following examples.
Detailed Description
The present invention will be further described with reference to examples, in which various chemicals and reagents are commercially available unless otherwise specified.
Example one
100 parts of purified water, 20 parts of humic acid, 1 part of amino acid coupled iron, 1 part of amino acid coupled zinc, 1 part of amino acid coupled boron, 1 part of amino acid coupled molybdenum, 1 part of amino acid coupled copper, 5 parts of potassium alginate and 5 parts of nano silicon are sequentially added into a fermentation tank to prepare a mixed solution, 5 parts of citric acid is used for adjusting the pH value to 5.5, the mixed solution is heated to 35 ℃ under stirring, 15 parts of purple violet spore bacillus, 15 parts of beauveria bassiana, 1 part of fresh artemisia argyi grass liquid and 1 part of Chinese ash liquid are added, aerobic gas is injected into the bottom layer of the fermentation tank to carry out gas stirring and mechanical stirring, the fermentation time is 72 hours, and the disease-preventing compound microbial agent combining amino acid and humic acid is prepared.
Example two
100 parts of purified water, 30 parts of humic acid, 1.5 parts of amino acid coupled iron, 1.5 parts of amino acid coupled zinc, 2 parts of amino acid coupled boron, 1 part of amino acid coupled molybdenum, 1 part of amino acid coupled copper, 7 parts of potassium alginate and 8 parts of nano silicon are sequentially added into a fermentation tank to prepare a mixed solution, 6 parts of citric acid is used for adjusting the pH value to 7, the mixed solution is heated to 38 ℃ under stirring, 10 parts of trichoderma harzianum, 20 parts of purple violet spore bacteria, 1 part of castor leaf powder and 2 parts of peach leaf liquid are added, aerobic gas is injected into the bottom layer of the fermentation tank for gas stirring and mechanical stirring, and the fermentation time is 90 hours, so that the disease and insect prevention composite microbial agent with the amino acid and the humic acid combined is prepared.
EXAMPLE III
100 parts of purified water, 30 parts of humic acid, 1.5 parts of amino acid coupled iron, 1.5 parts of amino acid coupled zinc, 1.5 parts of amino acid coupled boron, 1.5 parts of amino acid coupled molybdenum, 1.5 parts of amino acid coupled copper, 5 parts of ammonium alginate and 10 parts of nano silicon are sequentially added into a fermentation tank to prepare a mixed solution, 8 parts of citric acid is used for adjusting the pH value to be 6.24, the mixed solution is heated to 37 ℃ under stirring, then 20 parts of trichoderma harzianum, 30 parts of bacillus thuringiensis, 1 part of mulberry leaf liquid and 1 part of masson pine liquid are added, aerobic gas is injected into the bottom layer of the fermentation tank for gas stirring and mechanical stirring, the fermentation time is 80 hours, and the disease and insect prevention composite microbial agent combining amino acid and humic acid is prepared.
Example four
100 parts of purified water, 40 parts of humic acid, 1 part of amino acid coupled iron, 1 part of amino acid coupled zinc, 2 parts of amino acid coupled boron, 1.5 parts of amino acid coupled molybdenum, 1 part of amino acid coupled copper, 1 part of amino acid coupled manganese, 8 parts of potassium alginate and 10 parts of nano silicon are sequentially added into a fermentation tank to prepare a mixed solution, 6 parts of citric acid is used for adjusting the pH value to 5.8, the mixed solution is heated to 37 ℃ under stirring, then 25 parts of bacillus thuringiensis, 15 parts of paecilomyces lilacinus, 2 parts of ailanthus leaf liquid and 1 part of tobacco powder are added, aerobic gas is injected into the bottom layer of the fermentation tank to carry out gas stirring and mechanical stirring, and the fermentation time is 80 hours, so that the disease and insect prevention composite microbial agent combining amino acid and humic acid is prepared.
EXAMPLE five
100 parts of purified water, 40 parts of humic acid, 1.5 parts of amino acid coupled iron, 1.5 parts of amino acid coupled zinc, 1.5 parts of amino acid coupled boron, 2 parts of amino acid coupled molybdenum, 1 part of amino acid coupled copper, 1.5 parts of amino acid coupled manganese, 6 parts of alginic acid essence and 6 parts of nano silicon are sequentially added into a fermentation tank to prepare a mixed solution, 5 parts of citric acid is used for adjusting the pH value to 6.6, the mixed solution is heated to 36 ℃ under stirring, then 20 parts of beauveria bassiana, 20 parts of paecilomyces lilacinus, 2 parts of fresh wormwood liquid and 2 parts of Chinese ash liquid are added, aerobic gas is injected into the bottom layer of the fermentation tank for gas stirring and mechanical stirring, the fermentation time is 96 hours, and the insect-preventing and disease-preventing compound microbial agent combining amino acid and humic acid is prepared.
EXAMPLE six
100 parts of purified water, 50 parts of humic acid, 1.5 parts of amino acid coupled iron, 1.5 parts of amino acid coupled zinc, 1 part of amino acid coupled boron, 1 part of amino acid coupled molybdenum, 2 parts of amino acid coupled copper, 2 parts of coupled amino acid manganese, 7 parts of sodium alginate and 7 parts of nano silicon are sequentially added into a fermentation tank to prepare a mixed solution, 8 parts of citric acid is used for adjusting the pH value to be 6.8, the mixed solution is heated to 38 ℃ under stirring, 10 parts of lilac violet spore bacteria, 20 parts of bacillus thuringiensis, 10 parts of paecilomyces lilacinus, 1.5 parts of folium alpiniae oxyphyllae and 1.5 parts of ginkgo leaf liquid are added, aerobic gas is injected into the bottom layer of the fermentation tank to carry out gas stirring and mechanical stirring, and the fermentation time is 84 hours, so that the disease-preventing and insect-preventing compound microbial agent combining amino acid and humic acid is prepared.
The using effect of the product prepared by the embodiment is as follows:
the prepared potassium fulvate compound microbial agent is subjected to a drip irrigation test on the basis of normal fertilization management (namely comparison) in greenhouse cucumber planting in garden villages of Shahe county gardens in Lingze county, Gansu province, and six treatments are carried out, and three repeated comparison experiments (the comparison is normal fertilization management of greenhouses, 200 kg/mu of organic fertilizer, 10 kg/mu of urea and 40 kg/mu of diammonium phosphate) have the following effects:
the prepared potassium fulvate compound microbial agent is subjected to a drip irrigation test on the basis of normal fertilization management (namely comparison) in cabbage planting in Zhengzhai village of Yangxia province, namely Yangyang province, and six treatments are performed, and three repeated comparison experiments (the comparison is normal fertilization management of a greenhouse, 180 kg/mu of organic fertilizer, 20 kg/mu of urea and 20 kg/mu of diammonium phosphate) are performed, so that the effects are as follows:
the prepared potassium fulvate compound microbial agent is subjected to a drip irrigation test on the basis of normal fertilization management (namely comparison) in the scattered cauliflower planting in the village of the Yangxiang village of the Xianguan village in the Ulmus of Lanzhou city of Gansu province, six treatments are carried out, and three repeated comparison experiments (the comparison is the normal fertilization management of a greenhouse, 180 kg/mu of organic fertilizer, 25 kg/mu of urea and 25 kg/mu of diammonium phosphate) are carried out, so that the effects are as follows:
finally, it should be noted that: the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the invention without departing from the spirit and scope of the invention.
Claims (4)
1. The disease-preventing and insect-preventing compound microbial agent combining amino acid and humic acid is characterized by comprising the following components in parts by weight:
20-50 parts of humic acid; 20-50 parts of a microbial agent; 5-10 parts of trace elements; 5-10 parts of alginic acid; 2-4 parts of biological disease-preventing and insect-preventing substances; 5-10 parts of nano silicon; 5-10 parts of citric acid and 100 parts of purified water; wherein the microbial agent is one or more of trichoderma harzianum, purple spore fungus, beauveria bassiana, bacillus thuringiensis, paecilomyces lilacinus and the like; the biological disease-preventing and insect-preventing substance is one or more of castor leaf powder, peach leaf liquid, mulberry leaf liquid, masson pine liquid, ailanthus leaf liquid, tobacco powder, artemisia fresh grass liquid, Chinese ash liquid, invar leaf liquid, ginkgo leaf liquid and the like.
2. The compound microbial agent for preventing diseases and insects, which combines amino acid and humic acid, according to claim 1, is characterized in that: the microelements are one or more of amino acid coupled iron, amino acid coupled zinc, amino acid coupled molybdenum, amino acid coupled copper, amino acid coupled manganese, amino acid coupled boron, amino acid coupled iron, zinc, molybdenum, copper, manganese, boron, etc.
3. The compound microbial agent for preventing diseases and insects, which combines amino acid and humic acid, according to claim 1, is characterized in that: the alginic acid is one or more of potassium alginate, ammonium alginate, sodium alginate, alginic acid essence, etc.
4. A method for preparing the compound microbial agent for preventing diseases and insects by combining amino acid and humic acid according to claims 1-3, which is characterized in that: the method comprises the following steps of sequentially adding purified water, humic acid, trace elements, alginic acid and nano silicon into a fermentation tank according to the weight parts of the raw materials in claim 1 to prepare a mixed solution, adjusting the pH value to 5.5-7 by using citric acid, heating to 35-38 ℃ under stirring, adding a microbial agent and a biological disease-preventing and insect-preventing substance, injecting aerobic gas into the bottom layer of the fermentation tank to perform gas stirring and mechanical stirring, and fermenting for 72-96 hours to obtain the disease-preventing and insect-preventing compound microbial agent combining amino acid and humic acid.
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| CN202111074060.4A CN113773138A (en) | 2021-09-14 | 2021-09-14 | Disease-preventing and insect-preventing compound microbial agent combining amino acid and humic acid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114988952A (en) * | 2022-07-08 | 2022-09-02 | 云南勐牛生物技术有限公司 | Nano silicon liquid fertilizer and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113135803A (en) * | 2021-06-08 | 2021-07-20 | 深圳市绿色农业生物科技有限公司 | Disease-preventing insect-preventing environment-friendly microbial water-soluble fertilizer |
| CN113277909A (en) * | 2021-06-08 | 2021-08-20 | 深圳市绿色农业生物科技有限公司 | Compound microbial agent of potassium fulvate |
-
2021
- 2021-09-14 CN CN202111074060.4A patent/CN113773138A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113135803A (en) * | 2021-06-08 | 2021-07-20 | 深圳市绿色农业生物科技有限公司 | Disease-preventing insect-preventing environment-friendly microbial water-soluble fertilizer |
| CN113277909A (en) * | 2021-06-08 | 2021-08-20 | 深圳市绿色农业生物科技有限公司 | Compound microbial agent of potassium fulvate |
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| 吴志行 等: "《环保型商品蔬菜生产技术》", vol. 1, 29 February 2004, 金盾出版社, pages: 220 - 224 * |
| 杨洪强: "《有机园艺》", vol. 1, 31 July 2005, 中国农业出版社, pages: 397 - 398 * |
| 郭松: "《宁夏西甜瓜栽培技术》", vol. 1, 31 August 2020, 宁夏人民出版社, pages: 256 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114988952A (en) * | 2022-07-08 | 2022-09-02 | 云南勐牛生物技术有限公司 | Nano silicon liquid fertilizer and preparation method thereof |
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