CN113750025A

CN113750025A - Plant composition with anti-aging effect and application of plant composition in cosmetics

Info

Publication number: CN113750025A
Application number: CN202111226446.2A
Authority: CN
Inventors: 梁锋; 熊中立; 邹同
Original assignee: Shanghai Zhengxin Biotechnology Co ltd
Current assignee: Shanghai Zhengxin Biotechnology Co ltd
Priority date: 2021-10-21
Filing date: 2021-10-21
Publication date: 2021-12-07

Abstract

The invention discloses a plant composition with an anti-aging effect and application thereof in cosmetics. A plant composition with an anti-aging effect is prepared from the following raw materials in parts by weight: 1-6 parts of rhizoma polygonati; 0.1-2 parts of lucid ganoderma; 0.1-1 part of figwort; and radix Paeoniae 0.1-1 weight parts. The plant composition disclosed by the invention uses herbal essence components, combines modern technology extraction, furthest reserves the natural effective cost of herbs, is mild and non-irritant, and has natural and fragrant smell. The plant composition has multiple composite effects of moisturizing, brightening skin, repairing, resisting aging, resisting inflammation, relieving, enhancing elasticity, fading fine lines and the like, and can be used as a basic raw material of various cosmetics.

Description

Plant composition with anti-aging effect and application of plant composition in cosmetics

Technical Field

The invention provides a preparation method and application of a plant composition, and particularly relates to a preparation method and application of a plant composition applied to an anti-aging cosmetic.

Background

As the concept of anti-aging is becoming more and more aware, the age structure of consumers purchasing anti-aging products tends to be younger, and more consumers will choose to purchase anti-aging products before the age of 25. Aging is often unavoidable with increasing age, and in the face of this aging we can do so with retardation.

However, in the present situation, because people accelerate aging due to work and life and some external factors, aging phenomena occur inside and outside the body of the young generation, and the embodiment on the skin is particularly obvious. These phenomena are physical warnings and can cause the physical and physical quality of the whole person to be greatly impaired, even affecting our work life and interpersonal interaction, so that a correct anti-aging means is necessary and beneficial to our physical and mental health. The possibility of achieving anti-aging is also increasing due to the development of scientific technology.

The plant cosmetics are different from other cosmetics, are green, natural, safe and reliable, come from nature, are pure and mild, have small toxic and side effects, and are safer and more reliable than chemical synthetic products; has complete categories and various formulations.

However, how to obtain the effective part with remarkable efficacy and how to perform compatibility needs a great deal of research.

Disclosure of Invention

The invention aims to provide a plant composition with an anti-aging effect and a preparation method thereof.

The invention also aims to provide the application of the plant composition in the field of cosmetics, and the plant composition has multiple composite effects of moisturizing, brightening skin, repairing, resisting aging, resisting inflammation, relieving, enhancing elasticity, fading fine lines and the like, and can be used as a basic raw material of various cosmetics.

The purpose of the invention is realized by the following technical scheme:

a plant composition is prepared from the following raw materials in parts by weight:

1-6 parts of rhizoma polygonati;

0.1-2 parts of lucid ganoderma;

0.1-1 part of figwort; and

0.1-1 weight part of peony.

Further, the plant composition with the anti-aging effect is prepared from the following raw materials in parts by weight:

3-6 parts of rhizoma polygonati;

0.1 to 1 weight part of lucid ganoderma;

0.3-1 part of figwort; and

radix Paeoniae 0.3-1 weight parts.

4-5 parts of rhizoma polygonati;

0.5-1 part by weight of lucid ganoderma;

0.3-0.5 part of figwort; and

radix Paeoniae 0.3-0.5 weight parts.

In addition, the plant composition with the anti-aging effect is prepared by the following process steps:

taking alcohol extracts of rhizoma polygonati, ganoderma lucidum, radix scrophulariae and radix paeoniae alba in a formula amount as active ingredients, dissolving the active ingredients in butanediol, 1.2 pentanediol, water and the like, and compounding to obtain rhizoma polygonati effective extract, ganoderma lucidum effective extract, radix scrophulariae effective extract and radix paeoniae alba effective extract;

the extraction process of the effective substances of the sealwort comprises the following steps:

1) processing rhizoma Polygonati according to its properties;

2) weighing rhizoma Polygonati in formula amount, pulverizing, and soaking in distilled water for 24 hr;

3) adding distilled water according to a certain liquid material proportion, performing reflux extraction, centrifuging and concentrating;

4) adding ethanol into the concentrated solution, filtering, centrifuging, and freeze drying to obtain crude product of rhizoma Polygonati effective components;

5) dissolving the crude product, mixing with chloroform-n-butanol, shaking vigorously for 30min to denature protein to obtain gel, and centrifuging to remove denatured protein in the middle layer;

6) adding activated carbon into the solution, decolorizing while stirring every 10min, adding anhydrous ethanol into the extractive solution to make ethanol concentration in the mixed solution reach 80%, standing, filtering, and centrifuging. The rhizoma polygonati active substance is obtained.

The extraction process of the ganoderma lucidum effective substances comprises the following steps:

1) crushing the ganoderma lucidum according to the formula amount, adding water with the amount 30 times of the medicinal material amount, and soaking the medicinal material for 1 hour;

2) heating to boil, reflux extracting for 4 hr, filtering while hot, and standing the extractive solution;

3) adding the filter residue into the extraction container again, adding water 30 times the amount of the medicinal materials again, heating to boil, reflux extracting for 3 hr, filtering while hot, and standing the extractive solution alone;

4) and respectively concentrating the two extracting solutions in vacuum to recover the solvent, recording the volume of the recovered solvent, and respectively placing 10mL of the recovered solvent in an evaporating dish, placing the evaporating dish in an electric heating air blowing drying oven and drying to constant weight.

5) Mixing the rest two extractive solutions, vacuum concentrating until the relative density of the concentrated solution is above 1.05g/mL, and drying in a vacuum drying oven at 65 deg.C until the water content is below 8%. The Ganoderma effective components are obtained.

The extraction process of the effective substances of the figwort roots comprises the following steps:

1) processing radix scrophulariae according to its properties;

2) weighing figwort roots according to the formula ratio, crushing, sieving with a 80-mesh sieve, and adding distilled water to soak for 4 hours;

3) heating to boil, reflux extracting for 2h, filtering while hot, and vacuum filtering and concentrating the extractive solution;

4) filtering the concentrated solution with macroporous resin, adding ethanol, filtering, centrifuging, and freeze drying to obtain radix scrophulariae effective component.

The extraction process of the peony effective substances comprises the following steps:

1) slicing, drying and crushing the fresh radix paeoniae alba of the formula amount to obtain a radix paeoniae alba raw material;

2) soaking the radix Paeoniae raw material in ethanol solution, simultaneously performing ultrasonic extraction, centrifuging after completion, and taking supernatant as extractive solution;

3) removing ethanol in the extracting solution by adopting a reduced pressure distillation mode to obtain a peony root extract;

4) passing the radix Paeoniae extract through macroporous adsorbent resin, and washing the macroporous adsorbent resin with eluent to obtain eluent;

5) removing the eluent in the eluent by adopting a reduced pressure distillation mode to obtain a concentrated solution, and carrying out vacuum freeze drying on the concentrated solution to obtain a purified powdery or pasty radix paeoniae alba extract.

The separated and extracted effective components of the sealwort, the lucid ganoderma, the figwort and the peony are compounded according to a certain process and proportion to obtain the plant composition with the anti-aging effect.

The plant composition eye cream is prepared by compounding and composing the separated and extracted rhizoma polygonati effective component, the lucid ganoderma effective component, the figwort effective component and the peony effective component according to a certain process and proportion. Adding cosmetically acceptable adjuvants or adjuvant ingredients, and making into eye cream, essence, facial mask, water emulsion, and facial cream.

Further, the eye cream containing the plant composition with the anti-aging effect is prepared from the following raw materials and auxiliary materials in proportion:

the component A comprises: deionized water, butanediol, HA 1% solution, allantoin, CARBOPOL 940, glycerol, BIOPHILIC S, betaine NMF-50, and p-hydroxyacetophenone;

the component B comprises: phytosterol oleate, shea butter, squalane, tricaprylin, isononyl isononanoate, polydimethylsiloxane, cetostearyl alcohol, tocopheryl acetate (non-natural), ethylene glycol monostearate, olive oil;

and the component C is as follows: triethanolamine, SEP 400, 1.2-hexanediol, a plant composition and essence.

The preparation method of the eye cream of the plant composition with the anti-aging effect comprises the following steps:

1) weighing phase A, heating to 75-85 deg.C, maintaining the temperature, and stirring until dispersed uniformly.

2) And weighing the phase B, adding the phase B into the phase A, and uniformly stirring.

3) Stirring and cooling to 40-45 ℃, and adding the phase C in sequence and stirring uniformly.

Optimally, the preparation method of the eye cream of the plant composition with the anti-aging effect comprises the following steps:

1) weighing deionized water, butanediol, HA 1% solution, allantoin, CARBOPOL 940, glycerol, BIOPHILIC S, betaine NMF-50, and p-hydroxyacetophenone. Heating to 85 deg.C, maintaining the temperature, and stirring until the dispersion is uniform.

2) Weighing phytosterol oleate, shea butter, squalane, tricaprylin, isononyl isononanoate, polydimethylsiloxane, cetostearyl alcohol, tocopheryl acetate (non-natural), glycol monostearate, and olive oil. Adding into phase A, stirring.

3) Stirring, cooling to 45 deg.C, sequentially adding triethanolamine, SEP 400, 1.2-hexanediol, plant composition, and essence, and stirring.

The invention provides the use of said plant composition for the preparation of an eye cream product.

The invention also provides the application of the plant composition in preparing other cosmetics.

By means of the technical scheme, the invention has the following advantages and beneficial technical effects:

1) the invention uses the herbal essence components, combines the modern technology for extraction, furthest reserves the natural effective cost of the herbs, is mild and non-irritant, and has natural and fragrant smell. Has multiple composite effects of moisturizing, brightening skin, repairing, resisting aging, resisting inflammation, relieving, enhancing elasticity, and removing fine lines.

2) Through test experiments, the plant composition product does not show cytotoxicity; can obviously improve the activity of SOD and has antioxidant effect; can obviously improve the content of cell anti-aging related protein and has anti-aging effect; human tests show that the test sample has the effect of improving the fishtail line after two weeks.

Drawings

FIG. 1 is a graph of cell viability.

FIG. 2 is a graph showing the change in the concentration detected by cell morphology.

FIG. 3 is a summary chart of results of immunofluorescence assay of cells after incubation and culture, Collagen I.

Fig. 4 is a summary of pictures of the Elastin detection results.

Detailed Description

The present invention will be described in more detail below with reference to specific preferred embodiments and examples of effects, but the present invention is not limited to the following examples.

The invention discloses a plant composition with an anti-aging effect and application thereof in cosmetics. The invention is prepared mainly from 1-6 parts by weight of rhizoma polygonati, 0.1-2 parts by weight of ganoderma lucidum, 0.1-1 part by weight of radix scrophulariae and 0.1-1 part by weight of Chinese herbaceous peony by a proper method.

1) processing rhizoma Polygonati according to its properties;

1) processing radix scrophulariae according to its properties;

The plant composition disclosed by the invention uses herbal essence components, combines modern technology extraction, furthest reserves the natural effective cost of herbs, is mild and non-irritant, and has natural and fragrant smell.

The plant composition has multiple composite effects of moisturizing, brightening skin, repairing, resisting aging, resisting inflammation, relieving, enhancing elasticity, fading fine lines and the like, and can be used as a basic raw material of various cosmetics.

Through test experiments: the botanical compositions of the invention exhibit no cytotoxicity; can obviously improve the activity of SOD and has antioxidant effect; can obviously improve the content of cell anti-aging related protein and has anti-aging effect; human body tests show that the test sample has the effect of improving the fishtail lines after four weeks.

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. It is intended that all modifications or alterations to the methods, procedures or conditions of the present invention be made without departing from the spirit and substance of the invention.

Obviously, many modifications, substitutions, and variations are possible in light of the above teachings of the invention, without departing from the basic technical spirit of the invention, as defined by the following claims.

Example 1

The formulation and preparation method of the plant composition corresponding to the eye cream in the embodiment 1 of the invention can be expressed as follows:

1. the formula is as follows:

the component A comprises:

65.91% deionized water, 4% butanediol, 5% HA 1% solution, 0.2% allantoin, 0.2% CARBOPOL 940, 4% glycerol, 1.5% BIOPHILIC S, 1% betaine NMF-50, 0.5% p-hydroxyacetophenone.

The component B comprises:

0.2% phytosterol oleate, 0.8% shea butter, 5% squalane, 4% tricaprylin, 1% isononyl isononanoate, 1.2% polydimethylsiloxane, 2.2% cetearyl alcohol, 0.15% tocopheryl acetate (non-natural), 0.5% ethylene glycol monostearate, 1% olive oil.

And the component C is as follows:

0.16% triethanolamine, 0.6% SEP 400, 0.5% 1.2-hexanediol, 1% plant composition, 0.08% essence.

2. The preparation method comprises the following steps:

1) weighing 65.91% deionized water, 4% butanediol, 5% HA 1% solution, 0.2% allantoin, 0.2% CARBOPOL 940, 4% glycerol, 1.5% BIOPHILIC S, 1% betaine NMF-50, and 0.5% p-hydroxyacetophenone. Heating to 85 deg.C, maintaining the temperature, and stirring until the dispersion is uniform.

2) Weighing 0.2% phytosterol oleate, 0.8% shea butter, 5% squalane, 4% tricaprylin, 1% isononyl isononanoate, 1.2% polydimethylsiloxane, 2.2% cetearyl alcohol, 0.15% tocopheryl acetate (non-natural), 0.5% ethylene glycol monostearate, and 1% olive oil. Adding into phase A, stirring.

3) Stirring and cooling to 45 deg.C, and sequentially adding 0.16% triethanolamine, 0.6% SEP 400, 0.5% 1.2-hexanediol, 1% plant composition, and 0.08% essence. And (4) stirring uniformly.

Example 2

The formula and the preparation method of the essence corresponding to the plant composition in the embodiment 2 of the invention can be expressed as follows:

1. the formula is as follows:

the component A comprises:

84% deionized water, 0.15% CARBOPOL 941POLYMER, 5% methyl propylene glycol, 5% butanediol, 0.03% Tremella polysaccharide, 0.5% urea, 1% betaine, 0.05% DISODIUM EDTA, 0.2% allantoin, 0.5% diglycerin, 0.5% p-hydroxyacetophenone, 1% nicotinamide.

The component B comprises:

0.1% carnosine, 0.5% beta-glucan, 0.5% bioglycan gum, 0.5% 1.2-hexanediol, 1% plant composition, 0.1% essence.

2. The preparation method comprises the following steps:

1) adding 84% deionized water, 0.15% CARBOPOL 941POLYMER, 5% methyl propylene glycol, 5% butanediol, 0.03% Tremella polysaccharide, 0.5% urea, 1% betaine, 0.05% DISODIUM EDTA, 0.2% allantoin, 0.5% diglycerin, 0.5% p-hydroxyacetophenone, and 1% nicotinamide into a beaker, heating, stirring, and dissolving. The temperature reached 85 ℃.

2) Stirring with stirring paddle at 100 min for 30min, cooling to 45 deg.C, adding carnosine 0.1%, beta-dextran 0.5%, bioglycan 0.5%, 1.2-hexanediol 0.5%, plant composition 1%, and essence 0.1%, stirring, measuring pH, discharging, and packaging.

Example 3

The formula and preparation method of the facial mask corresponding to the plant composition in the embodiment 3 of the invention can be expressed as follows:

1. the formula is as follows:

the component A comprises:

87% deionized water, 0.06% CARBOPOL 941POLYMER, 3% plant propylene glycol, 0.05% sodium hyaluronate (small molecule), 5% methyl propylene glycol, 1% betaine, 0.2% allantoin, 0.5% glyceryl polyether-26, 0.06% xanthan gum, 0.5% urea, 0.02% tremella polysaccharide, and 0.5% p-hydroxyacetophenone.

The component B comprises:

0.5 percent of biological carbohydrate gum, 0.5 percent of beta-glucan, 0.5 percent of 1, 2-hexanediol, 0.3 percent of dittany bark compound anti-allergic agent, 1 percent of plant composition and 0.01 percent of essence.

2. The preparation method comprises the following steps:

1) 87% of deionized water, 0.06% of CARBOPOL 941POLYMER, 3% of plant propylene glycol, 0.05% of sodium hyaluronate (small molecule), 5% of methyl propylene glycol, 1% of betaine, 0.2% of allantoin, 0.5% of glyceryl polyether-26, 0.06% of xanthan gum, 0.5% of urea, 0.02% of tremella polysaccharide and 0.5% of p-hydroxyacetophenone. Adding into a beaker one by one, heating and stirring for dissolving. The temperature reached 75 ℃.

2) Stirring with stirring paddle at 100 rpm for 20 min, cooling to 45 deg.C, and adding 0.5% biogel, 0.5% beta-dextran, 0.5% 1, 2-hexanediol, 0.3% cortex Dictamni Radicis compound antiallergic agent, 1% plant composition, and 0.01% essence. Stirring, measuring pH (pH: 5.8-6.0), discharging, and packaging.

The advantageous effects of the present invention are further illustrated by the effect test examples below.

Test example 1

The phytocomposition of the present invention is tested for keratinocyte toxicity.

The experimental method comprises the following steps:

1) cell inoculation: by 1 × 10⁴Cell/well inoculation Density cells were plated onto 96 well plates, incubators (37 ℃, 5% CO)₂) And incubated overnight.

2) Grouping experiments: the experiment was set up with a zero adjustment group, a solvent control group, a positive control group and a sample group. In the sample set, 8 concentration gradients were set for each sample, and 3 replicate wells were set for each concentration gradient.

3) Preparing liquid: sample working solutions with different concentrations were prepared according to the test concentration setting table (table 1).

TABLE 1 test concentration setting Table

4) Administration: and (3) administration is carried out when the cell plating rate in the 96-well plate reaches 40-60%. Adding 200 mu L of culture solution into each well of the solvent control group; adding 200 mu L of culture solution containing 10% DMSO into each well of the positive control group; adding 200 mu L of culture solution containing samples with corresponding concentrations into each well of the sample group; the zero-adjusted group was seeded without cells, and only 200. mu.L of cell culture medium was added. After completion of the administration, the 96-well plate was placed in an incubator (37 ℃ C., 5% CO)₂) Culturing in medium.

5) And (3) detection: after 24h of cell incubation culture, the supernatant was discarded, MTT working solution (0.5mg/mL) was added, incubation was carried out at 37 ℃ for 4h in the dark, after the incubation was completed, the supernatant was discarded, 150. mu. LDMSO was added to each well, and the OD value was read at 490 nm.

6) Calculating the relative activity of the cells: calculated according to the formula, relative cell viability (%) 100%;

7) and (3) morphological detection: cell inoculation: and (3) setting a sample group and a solvent control group, wherein each group is provided with two multiple holes. The cells were seeded at the corresponding seeding density in 24-well plates, incubators (37 ℃ C., 5% CO)₂) And incubated overnight.

Preparing liquid: according to the MTT detection result, determining the morphological observation concentration of the detection sample, and preparing the working solution of the test object with different concentrations. Administration: when the cell plating rate of the 24-pore plate reaches 40%, the drug is administered, the samples are added with the test substances with different concentrations, the solvent control group is added with the cell culture solution, and the incubator (37 ℃, 5% CO)₂) And (4) performing medium incubation for 24 h. And (3) cell observation: after incubation, cell morphology was observed under a microscope and photographed.

From the MTT of fig. 1 and the morphological results of fig. 2, it is believed that the sample plant composition, based on keratinocytes, did not exhibit significant cytotoxicity in the 3% concentration range.

Test example 2

The antioxidant efficacy of the plant composition is detected.

The advantageous effects of the present invention are further illustrated by the following test examples.

The experimental groups are shown in table 2.

TABLE 2 Experimental groups

The experimental method comprises the following steps:

1) cell inoculation: by 2.5X 10⁵Cell/well inoculation Density cells were plated onto 6 well plates, incubators (37 ℃, 5% CO)₂) And incubated overnight.

2) Administration: according to Table 10 experimental design, when the cell plating rate in the 6-hole plate reaches 40% -60%, the medicine is administered in groups, each hole is loaded with 2mL, and each group is provided with 3 multiple holes. After completion of the administration, the 6-well plate was placed in an incubator (37 ℃ C., 5% CO)₂) And culturing for 24 h.

3) UVB irradiation: after PBS washing of the cells, the groups requiring UVB irradiation were divided into groups of 300mJ/cm according to the experiment²UVB irradiation of (2) in an incubator (37 ℃, 5% CO)₂) After incubation for 24 h.

4) And (3) detecting the SOD activity: the cell culture supernatant was aspirated, the cells were scraped off with a cell scraper, 1ml of pbs was added, and then the SOD activity was detected according to the SOD detection kit instructions.

5) And (4) analyzing results: SOD activity values of each group are summarized, t-test statistical analysis is adopted among the groups, P < 0.05 shows that the difference is obvious, and P < 0.01 shows that the difference is extremely obvious.

According to the specific experimental method, the SOD activity was tested, and the test results are shown in tables 3 and 4.

TABLE 3 summary of SOD activity results

Remarking: 1.# denotes p < 0.05 compared to BC; # indicates p < 0.01 compared to BC; 2.^＊representing p < 0.05 compared with NC;^＊＊p < 0.01 in comparison with NC; 3. tangle-solidup represents p < 0.01 compared to 3% botanical composition; 4.Δ means p < 0.01 compared to 1.5% plant composition.

Table 3 shows that the SOD activity of the 3% plant composition of the sample was significantly increased compared to the NC group; the SOD activity of 1.5 percent of the plant composition of the sample is obviously increased; the SOD activity of the plant composition of 0.5 percent of the sample is obviously increased. In addition, the SOD activity of the plant composition of 0.5 percent is obviously increased compared with that of the plant composition of 3 percent; the SOD activity of the 0.5% plant composition was significantly increased compared to the 1.5% plant composition.

TABLE 4 summary of SOD activity results

Remarking: 1.# denotes p < 0.05 compared to BC; # indicates p < 0.01 compared to BC; 2.^＊representing p < 0.05 compared with NC;^＊＊represents p < 0.01: 3. tangle-solidup represents p < 0.01: 4.Δ means p < 0.01 compared to 1.5% plant composition.

Table 4 shows that the SOD activity of the 0.2% plant composition of the sample was significantly increased compared to the NC group; SOD activity of 0.75% of the plant composition of the sample is obviously increased; SOD activity of 1% plant composition of the sample is obviously increased; the SOD activity of the 0.75% plant composition of the sample was significantly increased compared to the 0.2% plant composition; sample 1% plant composition had significantly increased SOD activity.

Test example 3

The anti-aging efficacy of the plant composition is detected.

The experimental groups are shown in table 5.

Remarking: there was no positive control for the measurement of the Elastin protein content.

The experimental method comprises the following steps:

1. immunofluorescence method

1) Cell inoculation: cells were seeded at a seeding density of 4X 104/well in 24-well plates and incubated overnight in an incubator (37 ℃ C., 5% CO 2).

2) Administration: according to the experimental design shown in table 16, when the plating rate of the cells in the 24-well plate reaches 40% -60%, the drug is administered in groups, each well is loaded with 1mL, and each group is provided with 3 multiple wells. After completion of the administration, the 24-well plate was placed in an incubator (37 ℃ C., 5% CO)₂) And culturing for 24 h.

3) After the incubation, 1mL of 4% paraformaldehyde was added to the 24-well plate to fix the cells for 15min, followed by washing with PBS 3 times/5 min.

4) And (3) sealing: 200 mu L/hole of goat serum is added dropwise, and the mixture is sealed for 60min at room temperature.

5) Incubating the primary antibody: the goat serum blocking solution was discarded, and primary antibody (200. mu.L/well) diluted in the appropriate ratio was added dropwise (goat serum dilution) and incubated overnight at 4 ℃.

6) Incubation of secondary antibody: washing with PBS for 3 times/5 min, adding diluted fluorescent secondary antibody (200 μ L/well), and incubating at room temperature in dark for 1 h.

7) Nuclear dyeing: wash 3 times/5 min with PBS, add Hochest33342 (200. mu.L/well) dropwise, and incubate for 5min at room temperature.

8) Sealing: the slide was picked up with a needle, a drop of anti-quencher was added dropwise to the slide, and the slide was inverted on the slide.

9) And (4) observing and photographing: pictures were taken by fluorescence microscope within 24 h.

2. Gene detection method

1) Cell inoculation: by 2X 10⁵Cell/well inoculation Density cells were plated onto 6 well plates, incubators (37 ℃, 5% CO)₂) And incubated overnight.

2) Administration: according to the experimental design shown in table 16, when the plating rate of cells in the 6-well plate reaches 40% -60%, the administration is carried out in groups, each well is loaded with 2mL, and each group is provided with 3 multiple wells. After completion of the administration, the 6-well plate was placed in an incubator (37 ℃ C., 5% CO)₂) And culturing for 24 h.

3) Gene detection: washing twice with 2 mL/well PBS, adding 1mL RNAioso Plus per well, blowing to crack cell, and collecting sample. RNA extraction, reverse transcription and fluorescent quantitative PCR detection are carried out according to the kit specification, and a 2-delta CT method is adopted for calculating results.

4) And (4) result statistical analysis: and (3) applying GraphPad Prism Program software to map, and performing t-test statistical analysis on the sample group and the control group, wherein p < 0.05 indicates that the difference is obvious, and p < 0.01 indicates that the difference is extremely obvious.

Immunofluorescence results: after the incubation culture is finished, the cells are subjected to immunofluorescence detection, and the results of the Collagen I are summarized in a figure 3 and a table 6.

Table 6 is a summary table of Collagen I test results (IOD values)

TABLE 6 Collagen I test results (IOD values) summary Table

Sample name	Relative IOD mean value	SD	P value

PC	2.13	0.16	0.000^＊＊
				1.5% botanical composition	1.94	0.38	0.013^＊
1% botanical composition	2.06	0.22	0.001^＊＊
				0.5% of plant composition	1.71	0.21	0.004^＊＊

Remarking:^＊p < 0.05:^＊＊p < 0.01, in comparison with BC.

The results of the Elastin assay are summarized in FIG. 4 and Table 7.

Table 7 is a summary table of Elastin detection results (IOD values)

Sample name	Relative IOD mean value	SD	Pvalue
				BC	1.00	0.08	/
1.5% botanical composition	1.65	0.30	0.022^＊
				1% botanical composition	1.92	0.12	0.000^＊＊
0.5% of plant composition	1.56	0.10	0.002^＊＊

Remarking:^＊p < 0.05, compared to BC;^＊＊p < 0.01, in comparison with BC.

Table 8 is a summary table of gene testing

Remarking:^＊p < 0.05, compared to BC;^＊＊p < 0.01, in comparison with BC.

Gene results:

based on an experimental method, the affected fibroblasts are subjected to RNAioso Plus treatment and then collected, and RNA extraction, reverse transcription and fluorescent quantitative PCR operation are carried out according to the kit specification. The results are summarized in Table 8.

And (3) knotting: compared with the BC group, the PC group has obvious effect of improving the expression of human fibroblast cells Collagen I, Collagen III, Elastin and Fibrilin. Compared with the BC group, the sample plant composition has obvious inhibition effect on the expression of human fibroblast Elastin and Fibrillin genes and has obvious improvement effect on the expression of Collagen III gene under the administration dosage of 1.5 percent; under the dosage of 1 percent, the compound has obvious inhibition effect on the expression of human fibroblast Collagen I, Elastin and Fibrilin genes: has obvious inhibition effect on the expression of human fibroblast Collagen I and Fibrilin genes under the administration dosage of 0.5 percent.

Test example 4

The wrinkle-improving efficacy of the plant composition is detected; purpose of the experiment: the efficacy of the product in improving wrinkles after 4 weeks of use was evaluated.

The experimental method comprises the following steps:

1) at the first visit, subjects were instructed to perform the trial and signed an informed consent.

Subject condition:

the planned number of people to be grouped: 35 persons;

the screening number: 40 persons;

the number of people entering the group: 35 persons;

the number of completed people is: 34 persons;

the number of analyzed persons: 34 persons;

2) the subjects tested no skin care products in the morning. After visiting, the testee cleans the facial skin with clear water, and after cleaning, the testee enters a constant-temperature constant-humidity room to sit statically for 30 minutes. After 30 minutes, the test subjects are screened according to the test requirements, 35 subjects are screened into the group, and finally more than 30 subjects are ensured to be completed. After screening was complete, instrumental measurements were performed on the enrolled subjects.

3) After the test is completed, the subject is instructed to use the product, and after the subject listens to the instruction, the product is dispensed. Subjects used the test samples at home for 4 consecutive weeks.

During this period, the subject followed the instructions of the trial and performed a visit to the venue at 2 weeks and 4 weeks of continuous product use to complete the facial instrument test of step 2).

4) Observing test site tail-eye crow's feet (Primos): photographs were taken using Primos and fishtail area ratio parameters were analyzed.

5) Parameter interpretation: the smaller the area of the crow's feet is, the lighter the crow's feet is.

6) And (3) testing results: the test results, after 2 weeks of use of the "botanical composition" by the subject population (34), showed: after the test sample is used for 2 weeks, the area ratio parameter of the fishtail line in the test area is in a significant reduction trend (P < 0.001) compared with that before the test sample is used, which indicates that the test sample has the effect of improving the fishtail line for 2 weeks, and the improvement rate is 13.69%.

The test results, after 4 weeks of use of the "botanical composition" by the subject population (34), showed: after the test sample is used for 4 weeks, the area ratio parameter of the fishtail lines in the test area is in a significant reduction trend (P < 0.001) compared with that before the test sample is used, which indicates that the test sample has the effect of improving the fishtail lines for 4 weeks, and the improvement rate is 12.99%.

The raw data are shown in Table 9.

TABLE 9 original data for efficacy in wrinkle improvement

In conclusion, the invention uses the herbal essence components and combines the modern technology extraction, thereby maximally keeping the natural effective cost of the herbs, being mild and non-irritant, and having natural and fragrant smell. Has multiple composite effects of moisturizing, brightening skin, repairing, resisting aging, resisting inflammation, relieving, enhancing elasticity, and removing fine lines.

Through test experiments, the plant composition of the product of the invention does not show cytotoxicity; can obviously improve the activity of SOD and has antioxidant effect; can obviously improve the content of cell anti-aging related protein and has anti-aging effect; human body tests show that the test sample has the effect of improving the fishtail lines after four weeks.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.

Claims

1. The plant composition with the anti-aging effect is characterized by being prepared from the following raw materials in parts by weight:

1-6 parts of rhizoma polygonati;

0.1-2 parts of lucid ganoderma;

0.1-1 part of figwort; and

0.1-1 weight part of peony.

2. The plant composition with anti-aging effect as claimed in claim 1, which is prepared from the following raw materials in parts by weight:

3-6 parts of rhizoma polygonati;

0.1 to 1 weight part of lucid ganoderma;

0.3-1 part of figwort; and

radix Paeoniae 0.3-1 weight parts.

3. The plant composition with the anti-aging effect as claimed in claim 2, which is prepared from the following raw materials in parts by weight:

4-5 parts of rhizoma polygonati;

0.5-1 part by weight of lucid ganoderma;

0.3-0.5 part of figwort; and

radix Paeoniae 0.3-0.5 weight parts.

4. The plant composition with anti-aging effect as claimed in any one of claims 1 to 3, which is prepared by the following process steps:

1) taking alcohol extracts of rhizoma polygonati, ganoderma lucidum, radix scrophulariae and radix paeoniae alba in a formula amount as active ingredients, dissolving the active ingredients in butanediol, 1.2 pentanediol, water and the like, and compounding to obtain rhizoma polygonati effective extract, ganoderma lucidum effective extract, radix scrophulariae effective extract and radix paeoniae alba effective extract;

the process for extracting rhizoma polygonati effective substances comprises the following steps:

processing rhizoma Polygonati according to its properties; weighing rhizoma Polygonati in formula amount, pulverizing, and soaking in distilled water for 24 hr; adding distilled water according to a certain liquid material proportion, performing reflux extraction, centrifuging and concentrating; adding ethanol into the concentrated solution, filtering, centrifuging, and freeze drying to obtain crude product of rhizoma Polygonati effective components; dissolving the crude product, mixing with chloroform-n-butanol, shaking vigorously for 30min to denature protein to obtain gel, and centrifuging to remove denatured protein in the middle layer; adding activated carbon into the solution, decolorizing while stirring every 10min, adding anhydrous ethanol into the extractive solution to make ethanol concentration in the mixed solution reach 80%, standing, filtering, and centrifuging to obtain rhizoma Polygonati effective component;

the extraction process of the ganoderma effective substances comprises the following steps:

crushing the ganoderma lucidum according to the formula amount, adding water with the amount 30 times of the medicinal material amount, and soaking the medicinal material for 1 hour; heating to boil, reflux extracting for 4 hr, filtering while hot, and standing the extractive solution; adding the filter residue into the extraction container again, adding water 30 times the amount of the medicinal materials again, heating to boil, reflux extracting for 3 hr, filtering while hot, and standing the extractive solution alone; respectively concentrating the two extracting solutions in vacuum to recover the solvent, recording the volume of the recovered solvent, and respectively placing 10mL of the recovered solvent in an evaporating dish, placing the evaporating dish in an electric heating air blast drying oven and drying the evaporating dish to constant weight; mixing the rest two extractive solutions, vacuum concentrating until the relative density of the concentrated solution is above 1.05g/mL, and drying in a vacuum drying oven at 65 deg.C until the water content is below 8% to obtain Ganoderma effective component;

the extraction process of the effective substances of the figwort is as follows:

processing radix scrophulariae according to its properties; weighing figwort roots according to the formula ratio, crushing, sieving with a 80-mesh sieve, and adding distilled water to soak for 4 hours; heating to boil, reflux extracting for 2h, filtering while hot, and vacuum filtering and concentrating the extractive solution; filtering the concentrated solution with macroporous resin, adding ethanol, filtering, centrifuging, and freeze drying to obtain radix scrophulariae effective substance;

slicing, drying and crushing the fresh radix paeoniae alba of the formula amount to obtain a radix paeoniae alba raw material; soaking the radix Paeoniae raw material in ethanol solution, simultaneously performing ultrasonic extraction, centrifuging after completion, and taking supernatant as extractive solution; removing ethanol in the extracting solution by adopting a reduced pressure distillation mode to obtain a peony root extract; passing the radix Paeoniae extract through macroporous adsorbent resin, and washing the macroporous adsorbent resin with eluent to obtain eluent; removing the eluent in the eluent by adopting a reduced pressure distillation mode to obtain a concentrated solution, and carrying out vacuum freeze drying on the concentrated solution to obtain the active substance of the Chinese herbaceous peony.

2) Compounding the separated and extracted rhizoma polygonati effective component, the lucid ganoderma effective component, the figwort effective component and the peony effective component according to a certain process and proportion to obtain the plant composition with the anti-aging effect;

3) adding cosmetically acceptable adjuvants or auxiliary components, and making into common cosmetics.

5. The plant composition with anti-aging effect as claimed in claim 4, wherein: in the extraction process of the rhizoma polygonati effective substances, the material-liquid ratio is 1: 10-30 g/ml; the extraction method is reflux extraction, the extraction temperature is 75-85 ℃, the extraction time is 1-3 h, and the extraction times are 1-3; the ethanol concentration is 95%; the mixing ratio of the chloroform to the n-butanol solution is 5: 1.

6. the plant composition with anti-aging effect as claimed in claim 4, wherein: in the extraction process of the effective substances of the figwort roots, the concentration of the ethanol is 90 percent.

7. The plant composition with anti-aging effect as claimed in claim 4, wherein: in the extraction process of the peony effective substance, the ultrasonic extraction time is 10min to 30min, the volume concentration of the ethanol solution is 50 percent to 90 percent, and the ratio of the volume of the ethanol solution to the mass of the peony root raw material is 5mL/g to 15 mL/g.

8. The plant composition with anti-aging effect as claimed in claim 4, wherein: the cosmetic comprises eye cream, essence, facial mask, water emulsion and facial cream.

9. Use of the botanical composition of claim 8 in the preparation of an anti-aging eye cream product, wherein: the addition amount of the plant composition is 0.5-2.5%, and the rest is adjuvant ingredients.

10. The plant composition with anti-aging effect as claimed in claim 9, wherein: the eye cream is prepared from the following raw materials and auxiliary materials in proportion:

and the component C is as follows: triethanolamine, SEP 400, 1.2-hexanediol, plant composition, essence;

the preparation process comprises the following steps: