CN113712049B - Application of saponin extract in preventing and treating red imported fire ants - Google Patents
Application of saponin extract in preventing and treating red imported fire ants Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
- A01N25/006—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits insecticidal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract
The invention belongs to the technical field of pest control, and particularly relates to application of a saponin extract in controlling solenopsis invicta. The invention discloses a Chinese honeylocust fruit extract prepared by taking Chinese honeylocust fruit pods as raw materials, which has remarkable control effect on red imported fire ants, the correction and control effects on the red imported fire ants on the 35 th day by using 0.005% of Chinese honeylocust fruit extract bait are all more than 80%, and compared with chemical pesticides, the Chinese honeylocust fruit extract has slow-acting poisoning effect on the red imported fire ants, can ensure that active ingredients for killing the ants are effectively delivered among ant groups, achieves the effect of nest-falling and killing, is not easy to generate drug resistance, has good environmental compatibility, is safe and nontoxic to people and livestock, has important significance for promoting the recycling of agricultural wastes, and has wide application prospect in production practice.
Description
Technical Field
The invention belongs to the technical field of pest control, and particularly relates to application of a saponin extract in controlling solenopsis invicta.
Background
The red fire ant (Solenopsis invicta Buren) is one of the most dangerous invasive species, has strong fecundity and competitiveness, and diversified transmission paths. Influence the production of agriculture and forestry, destroy ecological balance, endanger social public safety and human life safety. At present, the red fire ants are mainly prevented and controlled by adopting methods such as chemical prevention, physical prevention, PIM (personal information management), plant quarantine inspection and the like, wherein the chemical prevention and control technology is the most widely applied prevention and control method at present, but because the red fire ants mostly build ant nests at a position close to a water source, the large-scale and long-term chemical prevention and control technology easily enables chemical components to escape into a water body and causes harm to aquatic organisms, and therefore, the research on the green and environment-friendly red fire ant prevention and control technology has important significance.
Gleditsia sinensis (Gleditsia sinensis Lam.) is a plant of Gleditsia of Leguminosae, has a long cultivation history, is a multipurpose ecological and economical local tree species, and can be widely used for landscaping and ecological afforestation. Is widely distributed in China and has rich plant resources. Currently, only a few of saponin reports insecticidal activity of saponin, and researches show that saponin alcohol extracts have effects on armyworms and pieris rapae (Liyuqi, liu Xianbright, huguanfang, and the like.) the insecticidal activity of saponin extracts and the mode of the saponin extracts on the armyworms are researched [ J ]. Southwest agrimony, 2012,25 (1): 161-16.); gleditsia sinensis fruit pod powder and the ethanol extract of the fruit pod have a significant Inhibitory effect on Meloidogyne incognita (Wen Y, chitwood D J, meyer S L F, inhibition effects of Gleditsia sinensis (Chinese honeynociceps) Power fructics and extracts on Meloidogyne incognita [ J ]. Journal of biology.2015, (37) 3. Chinese patent CN110250228A discloses that an insecticide prepared by mixing Chinese honeylocust and various plant Chinese herbal medicines has a good control effect on powdery aphids, diamondback moths, armyworms, cabbage caterpillars and other pests in vegetables. However, the conventional control agents containing the saponin extract and utilizing the insecticidal activity of saponin are still relatively few, and there is no report on whether the saponin extract has a control effect on red imported fire ants.
Disclosure of Invention
The invention aims to overcome the defects and shortcomings in the prior art and provide the plant source bait for preventing and controlling the solenopsis invicta.
The second purpose of the invention is to provide application of the saponin extract in preventing and treating red imported fire ants.
The second object of the present invention is to provide a method for preparing gleditsia sinensis or red fire ant bait.
The above purpose of the invention is realized by the following technical scheme:
the invention finds that the extract extracted from the plant Chinese honeylocust (Gleditiia sinensis Lam.) has obvious activity of poisoning the solenopsis invicta.
Therefore, the invention provides the application of the saponin extract in preventing and treating the solenopsis invicta.
Preferably, the saponin extract is prepared by taking saponin fruit pods as raw materials.
Preferably, the saponin extract is a water extract, an ethanol extract or a methanol extract of the fruit pod of saponin.
Preferably, the extraction method of the saponin extract comprises the following steps: oven drying fructus Gleditsiae Abnormalis pod, pulverizing, collecting powder, ultrasonic extracting with water or alcohol solution, mixing filtrates, and concentrating under reduced pressure to obtain fructus Gleditsiae Abnormalis extract.
Preferably, the drying temperature is 40-60 ℃, the crushing and sieving are carried out by a 30-40 mesh sieve, and the ultrasonic condition is 20-30 ℃ for ultrasonic 1h.
The invention also provides application of the saponin extract in preventing and treating the solenopsis invicta or preparing a solenopsis invicta preventing and treating preparation.
The invention also provides a gleditsia sinensis and red imported fire ant bait, which contains the gleditsia sinensis extract.
Preferably, the saponin extract is contained in an amount of 0.001 to 99% by weight.
Preferably, the saponin extract is contained in an amount of 0.003 to 0.1% by weight.
More preferably, the saponin extract is contained in an amount of 0.003 to 0.005% by weight.
The research of the invention shows that the saponin extract has good control effect on the solenopsis invicta, and the higher the concentration of the saponin extract is, the better the control effect on the solenopsis invicta is in principle, but the antifeedant phenomenon can be generated in consideration of different feeding characteristics of insects. The experiments of the present invention show that when the concentration of the saponin extract used is 0.1%, the red fire ants can produce antifeedant effect, so that the preferable content of the saponin extract is limited to 0.1% by weight in order to avoid unnecessary waste. However, the antifeedant effect of the saponin extract with high concentration on the solenopsis invicta can be solved by other auxiliary ways which do not influence the insecticidal effect, which are not the main problems solved by the invention, so detailed discussion is not made, but foreseeable that on the basis that the saponin extract obtained by the first research of the invention has good control effect on the solenopsis invicta, the saponin extract is used for preparing the bait for controlling the solenopsis invicta, and the content range of the saponin extract can be determined and selected within the range of 0.001-99%.
In order to balance the effect and the cost, the experimental data show that the control effect on the solenopsis invicta buren is over 80 percent when the concentration of the saponin extract is 0.005 percent, so that the better control effect on the solenopsis invicta buren can be achieved by controlling the weight ratio of the saponin extract to be 0.003 to 0.1 percent.
Particularly, the gleditsia sinensis solenopsis invicta bait is not limited to contain components such as bait, vegetable oil, attractant, preservative, sweetener and warning pigment, and can also be other different components containing gleditsia sinensis extract.
As an alternative embodiment, the gleditsia sinensis solenopsis invicta bait comprises the following raw materials in parts by weight: 0.003 to 0.1 percent of Chinese honeylocust fruit extract, 60 to 70 percent of bait, 5 to 10 percent of vegetable oil, 8 to 15 percent of attractant, 0.05 to 0.9 percent of preservative, 5 to 10 percent of sweetening agent and 0.05 to 0.1 percent of warning pigment.
Preferably, the bait is corn flour, brown rice flour, buckwheat flour or tapioca flour.
Preferably, the vegetable oil is rapeseed oil, olive oil, peanut oil, soybean oil, castor oil, corn oil or coconut oil.
Preferably, the attractant is fishbone powder, chicken bone powder or shrimp powder.
Preferably, the preservative is sorbic acid and salts thereof, paraben preservatives, benzoic acid and salts thereof, propionic acid and salts thereof, chloromethylisothiazolinone, methylisothiazolinone, boric acid or borax.
Preferably, the sweetener is saccharin, cyclamate, acesulfame potassium, high fructose syrup, soy oligosaccharides, fructooligosaccharides, lactulose oligosaccharides, aspartame, or alitame.
Preferably, the warning pigment is edible red pigment powder, edible green pigment powder and edible melanin powder.
The invention also provides a preparation method of the gleditsia sinensis and red fire ant bait, which comprises the following steps:
s1, uniformly mixing bait, vegetable oil, an attractant, a preservative and a sweetening agent to obtain an auxiliary material I;
s2, preparing a saponin solution from the saponin extract, atomizing the saponin solution, spraying the atomized saponin solution into the auxiliary material I, uniformly mixing, and drying to obtain a material II;
and S3, adding the warning pigment into the material II, and uniformly mixing to obtain the gleditsia sinensis and solenopsis invicta bait.
Preferably, the mixing manner in steps S1, S2, S3 is stirring.
Preferably, the drying condition of the step S2 is shading and drying at 40 ℃.
Compared with the prior art, the invention has the following beneficial effects:
based on the research result of the invention, the Chinese honeylocust fruit extract has obvious control effect on the solenopsis invicta, and the correction control effect on the solenopsis invicta by using 0.005 percent of Chinese honeylocust fruit extract bait on the 35 th day is more than 80 percent; moreover, the gleditsia sinensis bait has slow effect on the red fire ants, so that the active ingredients for killing the ants can be effectively delivered among ant colonies.
The saponin extract is safe to the environment and free of pollution, and the saponin fruit pods are used as raw materials, are byproducts in the production process of saponin kernels, are rich in resources and contribute to maximization of saponin resource utilization.
Detailed Description
The present invention is further illustrated by the following specific examples in the specification, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1 70% ethanol extract of Gleditsia sinensis Lam and poisoning Activity against Solenopsis invicta
1. Oven drying Gleditsia sinensis fruit pod in an oven at 50 deg.C, pulverizing, sieving with 20 mesh sieve, collecting powder, extracting with 70% ethanol water solution (ethanol: water =70, 30, v/v) under ultrasonic condition at 25 deg.C for 1h for 3 times, mixing filtrates, concentrating under reduced pressure, and pulverizing to obtain Gleditsia sinensis extract.
And preparing the gleditsia sinensis extract into five liquid medicines with the concentration of 10mg/L, 20mg/L, 30mg/L, 40mg/L and 50mg/L by using pure water.
2. The poisoning effect of the solenopsis invicta is achieved by uniformly coating talcum powder on a dry plastic cup 4cm below the cup opening, reversely tapping the cup opening, and removing the redundant talcum powder. Selecting more than 25 ergates with the same size in a cup coated with pulvis Talci, starving for 6 hr, and adding into a water test tube. Adding corresponding medicament into each treatment group, adding pure water into the blank control group, repeating the treatment groups for 3 times, investigating the number of live and dead red fire ants in each group every 24h, and counting the death rate according to a formula until the death rate of the blank group worker ants is more than 20%.
As a result, as shown in tables 1 and 2, a significant toxicity was exhibited to Solenopsis invicta on the 6 th day after the treatment with 50mg/L of the extract of Gleditsia sinensis; therefore, the results of 6 to 10 days are statistically analyzed to obtain the virulence regression equation and LC of 6 to 10 days 50 The results are shown in Table 3, and the extracts of Gleditsia sinensis Lam on the Solenopsis invicta LC on days 6, 7, 8, 9 and 10 after the treatment 50 The values were 58.46mg/L, 43.45mg/L, 39.03mg/L, 37.53mg/L, 34.78mg/L, respectively.
TABLE 1 poisoning Activity of different concentrations of Gleditsia sinensis extract on Solenopsis invicta
TABLE 2 poisoning Activity of different concentrations of Gleditsia sinensis extract on Solenopsis invicta
TABLE 3 regression equation of toxicity of Gleditsia sinensis extract to Solenopsis invicta
Example 2 preparation of 0.003% Gleditsia Lam bait
1. Preparing a saponin extract: a 0.003% saponin extract was prepared by referring to the procedure of example 1 (extraction solvent was ethanol: water = 70.
2. 0.003 percent of gleditsia sinensis solenopsis invicta bait, and the components and the weight percentage of each component are as follows:
and (3) extracting gleditsia sinensis lam: 0.003%
Corn flour: 69.497%
Rapeseed oil: 10 percent of
Shrimp meal: 11 percent
Sorbic acid: 0.9 percent
Saccharin: 8.5 percent
Edible red pigment powder: 0.1 percent
3. The preparation method of the bait agent of the solenopsis invicta comprises the following steps:
(1) Weighing the raw materials according to the weight ratio of the raw materials;
(2) Firstly, dissolving weighed saponin extracts with a proper amount of pure water to obtain saponin solution;
(3) Then, adding the weighed corn flour, rapeseed oil, shrimp meal, sorbic acid and saccharin into a stirrer in sequence, and fully stirring to obtain an auxiliary material I;
(4) Then atomizing the prepared saponin solution, spraying the auxiliary material I, fully stirring and mixing, shading, and drying at the low temperature of 40 ℃ to obtain a material II;
(5) And adding the weighed edible red pigment powder into the material II, and fully stirring to obtain the 0.003% gleditsia sinensis and solenopsis invicta bait.
Example 3 preparation of 0.004% bait formulation for the production of Gleditsia sinensis Lam
1. Preparing a gleditsia sinensis extract: a 0.004% saponin extract was prepared according to the method of example 1 (extraction solvent was ethanol: water = 70.
2. 0.004 percent of gleditsia sinensis solenopsis invicta bait, and the components and the weight percentage of each component are as follows:
and (3) saponin extract: 0.004%
Flour: 68.896 percent
Soybean oil: 8 percent
Fish bone powder: 13 percent of
Potassium sorbate: 0.05 percent
High fructose syrup: 10 percent of
Edible melanin powder: 0.05 percent
3. The preparation method of the bait agent for solenopsis invicta comprises the following steps:
(1) Weighing the raw materials according to the weight ratio of the raw materials;
(2) Firstly, dissolving weighed saponin extracts with a proper amount of pure water to obtain saponin solution;
(3) Then adding the weighed flour, soybean oil, fishbone powder, potassium sorbate and high fructose syrup into a stirrer in sequence for fully stirring to obtain an auxiliary material I;
(4) Then atomizing the prepared saponin solution, spraying the auxiliary material I, fully stirring and mixing, shading, and drying at the low temperature of 40 ℃ to obtain a material II;
(5) And adding the weighed edible melanin powder into the material II, and fully stirring to obtain the 0.004% termite-killing bait for the solenopsis invicta.
Example 4 preparation of 0.005% Gleditsia sinensis Lam bait
1. Preparing a saponin extract: a 0.005% saponin extract was prepared according to the method of example 1 (extraction solvent was ethanol: water = 70.
2. 0.005% of gleditsia sinensis solenopsis invicta bait, which comprises the following components in percentage by weight:
and (3) saponin extract: 0.005 percent
Brown rice powder: 69.995 percent
Olive oil: 10 percent of
Chicken bone powder: 9 percent of
Sodium benzoate: 0.9 percent
Fructo-oligosaccharide: 10 percent of
Edible green pigment powder: 0.1 percent of
3. The preparation method of the bait agent of the solenopsis invicta comprises the following steps:
(1) The raw materials are weighed according to the weight ratio of the raw materials.
(2) Firstly, dissolving weighed saponin extracts with a proper amount of pure water to obtain saponin solution;
(3) Then, adding the weighed brown rice powder, olive oil, chicken bone powder, sodium benzoate and fructo-oligosaccharide into a stirrer in sequence for fully stirring to obtain an auxiliary material I;
(4) Then atomizing the prepared saponin solution, spraying the auxiliary material I, fully stirring and mixing, shading, and drying at the low temperature of 40 ℃ to obtain a material II;
(5) And adding the weighed edible green pigment powder into the material II, and fully stirring to obtain the 0.005% gleditsia sinensis solenopsis ant killing bait.
Example 5 preparation of 0.005% Gleditsia sinensis Lam bait
1. Preparing a gleditsia sinensis extract: reference is made to the procedure of example 1, except that a 0.005% saponin extract is prepared using a pure aqueous solution.
2. 0.005% of gleditsia sinensis bait, which comprises the following components in percentage by weight:
and (3) saponin extract: 0.005 percent
Buckwheat flour: 68.896 percent
Peanut oil: 8 percent of
Fish bone powder: 13 percent of
Calcium propionate: 0.05 percent
Soybean oligosaccharide: 10 percent
Edible red pigment powder: 0.053%
3. The procedure for the preparation of bait from solenopsis invicta was the same as in example 4.
Example 6 preparation of 0.005% Gleditsia Lamiacea bait
1. Preparing a gleditsia sinensis extract: reference is made to the procedure of example 1, except that 0.005% of the extract of gleditsia sinensis lam is prepared using a 100% ethanol solution.
2. 0.005% of gleditsia sinensis bait, which comprises the following components in percentage by weight:
gleditsia sinensis fruit pod extract: 0.005 percent
Cassava flour: 69.845 percent
Coconut oil: 5 percent of
Chicken bone powder: 15 percent of
Propyl p-hydroxybenzoate: 0.05 percent
A sweetener: 0.05 percent
Edible melanin powder: 0.13 percent
3. The procedure for the preparation of bait from solenopsis invicta was the same as in example 4.
Example 7.005% preparation of Gleditsia sinensis Formica Fusca bait formulation
1. Preparing a saponin extract: reference is made to the procedure of example 1, except that 0.005% of the extract of Gleditsia sinensis Lam was prepared using a 100% methanol solution.
2. 0.005% of gleditsia sinensis solenopsis invicta bait, which comprises the following components in percentage by weight:
gleditsia sinensis fruit pod extract: 0.005 percent
Cassava flour: 69.845 percent
Coconut oil: 5 percent
Chicken bone powder: 15 percent of
Propyl p-hydroxybenzoate: 0.05 percent
A sweetener: 0.05 percent
Edible melanin powder: 0.13 percent
3. The procedure for the preparation of the bait formulation for solenopsis invicta is the same as in example 4.
Example 8.005% preparation of bait for killing Ant of Gleditsia sinensis Lam
1. Preparing a gleditsia sinensis extract: a 0.005% saponin extract was prepared according to the method of example 1 (extraction solvent was ethanol: water = 70.
2. 0.005% of gleditsia sinensis solenopsis invicta bait, which comprises the following components in percentage by weight:
and (3) extracting gleditsia sinensis lam: 0.005 percent
Brown rice powder: 69.995 percent
Olive oil: 10 percent of
Chicken bone powder: 9 percent of
Boric acid: 0.9 percent
Fructo-oligosaccharide: 10 percent of
Edible green pigment powder: 0.1 percent of
3. The procedure for the preparation of bait from solenopsis invicta was the same as in example 4.
Example 9 preparation of 0.005% bait formulation for killing Formica rufa
1. Preparing a gleditsia sinensis extract: a 0.005% saponin extract was prepared according to the method of example 1 (extraction solvent was ethanol: water = 70.
2. 0.005% of gleditsia sinensis solenopsis invicta bait, which comprises the following components in percentage by weight:
and (3) saponin extract: 0.005 percent
Cassava flour: 69.845 percent
Coconut oil: 5 percent of
Chicken bone powder: 15 percent of
Borax: 0.05 percent
Sweet taste agent: 10 percent of
Edible green pigment powder: 0.1 percent of
3. The procedure for the preparation of bait from solenopsis invicta was the same as in example 4.
Example 10.005% preparation of bait for killing Ant of Gleditsia sinensis Lam
1. Preparing a saponin extract: a 0.005% saponin extract was prepared according to the method of example 1 (extraction solvent was ethanol: water = 70.
2. 0.005% of gleditsia sinensis solenopsis invicta bait, which comprises the following components in percentage by weight:
and (3) saponin extract: 0.005 percent
Cassava flour: 68.995 percent
Coconut oil: 10 percent
Chicken bone powder: 15 percent of
Borax: 0.5 percent
Boric acid: 0.4 percent
Sweet taste agent: 5 percent of
Edible red pigment powder: 0.1 percent of
3. The procedure for the preparation of bait from solenopsis invicta was the same as in example 4.
Example 11 field control Effect test
The bait prepared in the above examples 2 to 10 and the control group (blank vehicle: not containing gleditschia horrida extract compared with example 10) were used to conduct a solenopsis invicta control test in the field (the time of application, the time of day, the average temperature of 30 ℃ on the test day, no disastrous weather, soil, etc.) in the Guangdong Guangzhou Huadu district.
Experimental arrangement examples 2-10 bait and control groups (empty vehicle), 3 replicates. Uniformly spreading 20g of bait agent annularly with the ant hill as the center and the radius of 10 cm. The method comprises the steps of carrying out population quantity investigation on the red fire ants by a bait trapping method, namely putting ham sausages into a small plastic bottle, horizontally placing the plastic bottle during trapping to enable a bottle opening to be tightly attached to the ground, putting 4 trapping bottles (the diameter of the bottle opening is 8.5 cm) in each ant nest, enabling the trapping bottles to be about 7.0-8.0 cm away from the ant nest, collecting the trapping bottles after half an hour (dropping a small amount of acetone into the bottles during collection, covering the bottle caps), and carrying out indoor check on the quantity of the red fire ants collected in each bottle. The number of red fire ants collected in each bottle was investigated on the first 1 day, and the last 1 day, and on the 3 rd, 7 th, 14 th and 35 th days after the treatment, and the population reduction rate was calculated as follows:
as shown in table 4, the results of statistics show that 0.005% of gleditsia sinensis bait (example 4, example 5, example 6, example 7, example 9 and example 10) has a more than 80% corrected control effect on the solenopsis invicta on day 35, and the results of examples 9 and 10 show that the boric acid and borax added to the bait in examples 9 and 10 have a synergistic effect and a better control effect on the solenopsis invicta. The bait prepared in the embodiments 2 to 10 basically acts on the red fire ants on the about 35 th day, has slow action, can ensure that the active ingredients for killing the red fire ants are effectively and specially delivered among ant colonies, and avoids the red fire ants from being stimulated and diffused, thereby achieving the effect of nest-tilting and killing. In addition, the saponin extract used in the application is safe to the environment and harmless to human, livestock and aquatic organisms.
TABLE 4 field control Effect of Gleditsia sinensis bait against Solenopsis invicta
In conclusion, the alcohol extract and the water extract of the Chinese honeylocust fruit have good control effect on the red imported fire ants; the effect of the extract obtained by different extraction methods and extraction processes for preventing and treating the solenopsis invicta buren is slightly different. Experiments show that 0.005% of Chinese honeylocust fruit extract has good control effect on the red fire ants, the control effect of 70% of ethanol extract can reach about 84% in 35 days, is superior to about 81% of the control effect of 100% of ethanol extract, about 80% of the control effect of 100% of methanol extract and about 66% of the control effect of pure water extract, the control effect of preservatives in bait raw materials adopting borax and borax can reach about 90%, and the bait has synergistic effect on poisoning the red fire ants.
Therefore, the gleditsia sinensis and red imported fire ant bait provided by the invention has an obvious effect of preventing and treating red imported fire ants, is simple in components of prepared raw materials, easy to operate, difficult to generate drug resistance, good in environmental compatibility, safe and non-toxic to people and livestock, has an important significance in promoting recycling of agricultural wastes, and has a wide application prospect in production practice.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (2)
1. The application of the Chinese honeylocust fruit extract as an active ingredient in preventing and treating the red imported fire ants or preparing a red imported fire ant preventing and treating preparation is characterized in that the Chinese honeylocust fruit extract is prepared by taking Chinese honeylocust fruit pods as raw materials; the fructus Gleditsiae Abnormalis extract is water extract, ethanol extract or methanol extract of fructus Gleditsiae Abnormalis pod.
2. The use of claim 1, wherein the extract of Gleditsia sinensis Lam is prepared by oven drying and pulverizing fruit pod of Gleditsia sinensis Lam, collecting powder, ultrasonic extracting with water or alcohol solution, mixing filtrates, and concentrating under reduced pressure.
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| CN202111103623.8A CN113712049B (en) | 2021-09-18 | 2021-09-18 | Application of saponin extract in preventing and treating red imported fire ants |
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| WO2014086848A1 (en) * | 2012-12-04 | 2014-06-12 | Basf Agro B.V., Arnhem (Nl) | Compositions comprising a quillay extract and an insecticidal active ingredient |
| CN108633932A (en) * | 2011-10-04 | 2018-10-12 | 0903608 B.C.有限公司 | Injurious insect control formulation and manufacture and use its method |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5639794A (en) * | 1995-06-07 | 1997-06-17 | Proguard, Inc. | Use of saponin in methods and compositions for pathogen control |
| CN101189974A (en) * | 2007-11-30 | 2008-06-04 | 张颂声 | Safety environmental protection bait agent for red imported fire ant |
| CN101326920A (en) * | 2008-07-22 | 2008-12-24 | 惠州市南天生物科技有限公司 | Raticide |
| CN103997896A (en) * | 2011-12-16 | 2014-08-20 | 巴斯夫农业公司阿纳姆(荷兰)苏黎世分公司 | Synergistic fungicidal and insecticidal compositions comprising a quillay extract and an acacia negra extract |
| CN103053628B (en) * | 2012-03-22 | 2018-03-30 | 华南农业大学 | A kind of plant source fire ant bait agent |
| CN104757025B (en) * | 2014-01-02 | 2018-04-06 | 甘玉祥 | A kind of wide spectrum expelling worm plant and agricultural chemicals product |
| CN105410052A (en) * | 2015-11-26 | 2016-03-23 | 全椒县桃花湾果蔬种植专业合作社 | Dichocrocis punctiferalis attractive toxicant |
| CN108056099A (en) * | 2016-11-07 | 2018-05-22 | 江苏扬农化工股份有限公司 | Fluorine chlorine ether chrysanthemum ester and the effervescent tablet of arprocarb compounding and preparation method and application |
| CN107646897A (en) * | 2017-09-25 | 2018-02-02 | 江苏长龙国际贸易有限公司 | A kind of preparation method of cockroach attractant |
| CN111011367A (en) * | 2019-12-17 | 2020-04-17 | 安徽康宇生物科技工程有限公司 | Plant source bait for killing solenopsis invicta and preparation method thereof |
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| CN108633932A (en) * | 2011-10-04 | 2018-10-12 | 0903608 B.C.有限公司 | Injurious insect control formulation and manufacture and use its method |
| WO2014086848A1 (en) * | 2012-12-04 | 2014-06-12 | Basf Agro B.V., Arnhem (Nl) | Compositions comprising a quillay extract and an insecticidal active ingredient |
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