CN113699052A - 一株生产虾青素的重组酿酒酵母及其应用 - Google Patents

一株生产虾青素的重组酿酒酵母及其应用 Download PDF

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CN113699052A
CN113699052A CN202010430275.4A CN202010430275A CN113699052A CN 113699052 A CN113699052 A CN 113699052A CN 202010430275 A CN202010430275 A CN 202010430275A CN 113699052 A CN113699052 A CN 113699052A
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杨祖明
王竞辉
赵文娟
单雨瑶
吴计划
贾子樊
黎源
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Wanhua Chemical Sichuan Co Ltd
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Abstract

本发明公开了一株生产虾青素的重组酿酒酵母及其应用。本发明公开的重组酿酒酵母组成型表达β‑胡萝卜素酮化酶基因CrtW，诱导型表达β‑胡萝卜素羟化酶基因CrtZ，将中间产物β‑胡萝卜素合成为虾青素，所述β‑胡萝卜素酮化酶基因CrtW和β‑胡萝卜素羟化酶基因CrtZ的拷贝数之比为3:1‑3:2。利用本发明提供的重组酿酒酵母生产虾青素，虾青素的摇瓶发酵产量可达14.70mg/g DCW，同时纯度可达96.69％。

Description

一株生产虾青素的重组酿酒酵母及其应用
技术领域
本发明属于基因工程领域，涉及一株生产虾青素的重组酿酒酵母及其应用。
背景技术
虾青素是类胡萝卜素合成过程中的终级产物，在类胡萝卜素中抗氧化能力最强，被誉为“超级维生素E”。虾青素在藻类和海洋生物中以酯化的形式存在，在细菌和酵母中以游离形式存在。虾青素独特的化学结构，使其可以穿越细胞膜，穿过血脑屏障，因此相对其他类胡萝卜素具有更好的抗脂质氧化能力。由于虾青素能够有效的清除细胞内外的自由基，减少细胞损伤和衰老，因此在对心血管疾病、糖尿病和癌症等疾病方面具有较好的预防和治疗作用。
目前，虾青素的来源主要有化学合成和生物合成。化学合成虾青素过程复杂，过程中残留较多中间体，缺乏安全性，且合成的虾青素为三种构型的混合物，(3R,3’R)、(3S,3’S)、(3R,3’S)为1:1:2，抗氧化能力差，因此化学合成的虾青素目前只被批准用于水产养殖。生物合成虾青素的天然微生物包括雨生红球藻和红发夫酵母。红发夫酵母生产(3R,3’R)构型的虾青素，其抗氧化能力相对较低，目前主要用于饲料行业，用于改善动物的肉质和颜色，提高动物的免疫力。目前唯一被批准人类食用的是雨生红球藻生产的虾青素，其虾青素含量达到15-30mg/g DCW，在总类胡萝卜素中占99％以上，且雨生红球藻中的虾青素以(3S,3’S)形式存在，生物学活性很高。但是雨生红球藻的培养需要适宜的气候、长时间的光照以及大量的水资源，培养周期较长，导致生产成本较高。因此研究人员将目光转向了遗传背景清楚、基因操作工具丰富的大肠杆菌和酿酒酵母等微生物。虽然采用了大量的代谢工程手段和诱变技术，但是虾青素的产量仍然较低，离工业化生产仍然有不小的距离。因此，平衡虾青素在异源宿主内代谢路径的代谢通量和优化培养条件对提高虾青素生产尤为重要。
发明内容
本发明所要解决的技术问题是提高酿酒酵母中虾青素的产量和纯度。
为解决上述技术问题，本发明提供一株重组酿酒酵母，其组成型表达β-胡萝卜素酮化酶基因CrtW，诱导型表达β-胡萝卜素羟化酶基因CrtZ，将中间产物β-胡萝卜素合成为虾青素，所述β-胡萝卜素酮化酶基因CrtW和β-胡萝卜素羟化酶基因CrtZ的拷贝数之比为3:1-3:2；
该重组酿酒酵母为生产虾青素的工程菌株，为平衡酿酒酵母中虾青素路径碳源的流动，提高虾青素产量和纯度，得到工程菌株；所述平衡酿酒酵母中虾青素路径碳源的流动，提高虾青素产量和纯度，为通过调整β-胡萝卜素酮化酶基因CrtW和β-胡萝卜素羟化酶基因CrtZ的相对拷贝数，然后在不同类型启动子调控下，发酵过程中优选加入诱导剂来诱导CrtZ基因表达的时间和发酵的温度而实现的。
在一些实施方案中，上述重组酿酒酵母中，所述β-胡萝卜素酮化酶基因CrtW和β-胡萝卜素羟化酶基因CrtZ的拷贝数之比为3:2。
在一些实施方案中，上述任一所述的重组酿酒酵母中，所述组成型表达β-胡萝卜素酮化酶基因CrtW所用的组成型启动子为TDH3p、FBA1p和HXT7p中的一种或多种，TDH3p、FBA1p和HXT7p的序列分别如SEQ ID NO：1、SEQ ID NO：11和SEQ ID NO：20所示。
在一些实施方案中，上述任一所述的重组酿酒酵母中，所述诱导型表达β-胡萝卜素羟化酶基因CrtZ所用的诱导型启动子为Gal1和Gal10中的一种或多种，Gal1和Gal10的序列分别如SEQ ID NO：29和SEQ ID NO：39所示。
在一些实施方案中，上述任一所述的重组酿酒酵母中，所述β-胡萝卜素酮化酶基因CrtW为泡囊短波单胞菌(Brevundimonas vesicularis DC263)来源的CrtW，序列如SEQID NO:2所示。
在一些实施方案中，上述任一所述的重组酿酒酵母中，所述β-胡萝卜素羟化酶基因CrtZ为橙黄农杆菌(Agrobacterium aurantiacum)来源的CrtZ，序列如SEQ ID NO:30所示。
为解决上述技术问题，本发明还提供一种酿酒酵母的重组表达载体，包括组成型启动子及其控制下的β-胡萝卜素酮化酶基因CrtW，以及诱导型启动子及其控制下的β-胡萝卜素羟化酶基因CrtZ，所述β-胡萝卜素酮化酶基因CrtW和β-胡萝卜素羟化酶基因CrtZ的拷贝数之比为3:1-3:2。例如，将不同拷贝的β-胡萝卜素酮化酶基因CrtW和β-胡萝卜素羟化酶基因CrtZ连接到穿梭质粒上构建重组表达载体。
在一些实施方案中，上述重组表达载中，所述组成型启动子为TDH3p、FBA1p和HXT7p中的一种或多种，TDH3p、FBA1p和HXT7p的序列分别如SEQ ID NO：1、SEQ ID NO：11和SEQ ID NO：20所示。
在一些实施方案中，上述任一所述的重组表达载中，所述诱导型启动子为Gal1和Gal10中的一种或多种，Gal1和Gal10的序列分别如SEQ ID NO：29和SEQ ID NO：39所示。
在一些实施方案中，上述任一所述的重组表达载中，所述β-胡萝卜素酮化酶基因CrtW下游具有终止子，终止子为TDH2t、TEF2t和CYC1t中的一种或多种，TDH2t、TEF2t和CYC1t的序列分别如SEQ ID NO：3、SEQ ID NO：12和SEQ ID NO：21所示。
在一些实施方案中，上述任一所述的重组表达载中，所述β-胡萝卜素羟化酶基因CrtZ下游具有终止子，终止子为ADH1t和TPI1t中的一种或多种，ADH1t和TPI1t的序列分别如SEQ ID NO：31和SEQ ID NO：40所示。
在一些实施方案中，上述任一所述的重组表达载中，所述β-胡萝卜素酮化酶基因CrtW为泡囊短波单胞菌(Brevundimonas vesicularis DC263)来源的CrtW，序列如SEQ IDNO:2所示。
在一些实施方案中，上述任一所述的重组表达载中，所述β-胡萝卜素羟化酶基因CrtZ为橙黄农杆菌(Agrobacterium aurantiacum)来源的CrtZ，序列如SEQ ID NO:30所示。
在一些实施方案中，上述任一所述的重组表达载中，所述穿梭质粒是常规商业化PRS426质粒。
在一些实施方案中，上述任一所述的重组表达载中，所述重组表达载体为SEQ IDNO：58所示的PRS426-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t、SEQ IDNO：67所示的PRS426-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t和SEQ ID NO：73所示的PRS426-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-Gal10-CrtZ-TPI1t中的一种或多种，优选为PRS426-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-Gal10-CrtZ-TPI1t。
为解决上述技术问题，本发明还提供一种构建上述任一所述的重组酿酒酵母的方法，包括将上述任一所述的重组表达载体转入出发酿酒酵母中获得重组酿酒酵母的步骤；
所述出发酿酒酵母为能合成β-胡萝卜素的酿酒酵母，优选为Scy10026酿酒酵母。
为解决上述技术问题，本发明还提供一种生产虾青素的方法，包括将上述任一所述的重组酿酒酵母进行发酵培养，使得β-胡萝卜素酮化酶基因CrtW组成型表达并且在发酵培养过程中添加D-(+)-半乳糖诱导β-胡萝卜素羟化酶基因CrtZ表达以合成虾青素的步骤。
在一些实施方案中，上述生产虾青素的方法中，所述发酵培养包括将上述任一所述的重组酿酒酵母在发酵培养基中进行发酵培养，在发酵的第30-54小时加入终浓度2％的D-(+)-半乳糖，优选为在发酵的第36小时加入终浓度2％的D-(+)-半乳糖；
发酵培养基为4％葡萄糖的YPD培养基(组成为10g/L酵母膏，20g/L蛋白胨，40g/L葡萄糖，余量为水)；
发酵温度为20℃-30℃，优选20℃；
初始OD600可以为0.1，转速可以为250rpm。
在一些实施方案中，上述生产虾青素的方法中，在发酵培养之前，还具有将上述任一所述的重组酿酒酵母进行一级种子培养和二级种子培养的步骤；
所述一级种子培养包括将上述任一所述的重组酿酒酵母接入装有SC-Ura液体培养基的摇瓶中，在例如30℃、250rpm的条件下培养过夜；
所述二级种子培养包括将一级种子培养得到的一级种子转接至装有SC-Ura液体培养基的摇瓶中，在例如30℃、250rpm的条件下培养至OD600＝5-6的步骤。
所述一级种子的接种量为1％-2％。
为解决上述技术问题，本发明还提供上述任一所述的重组酿酒酵母和/或上述任一所述的重组表达载体在生产虾青素中的应用。
一种生产高纯度虾青素酿酒酵母工程菌株的构建：
本发明的有益效果是：虾青素代谢路径涉及众多基因的调控(如图1所示)，特别是其中的CrtW和CrtZ，本发明通过调整CrtW和CrtZ之间的相对拷贝数来平衡酿酒酵母中虾青素路径的碳源流动，并且将CrtW和CrtZ基因置于不同类型启动子的调控下，使得CrtW基因组成型表达，CrtZ基因诱导型表达，构建了重组酿酒酵母，同时进一步优化了重组酿酒酵母的发酵条件。本发明提高了虾青素的产量和纯度，摇瓶水平的虾青素生产可高达14.70mg/gDCW，虾青素纯度达到了96.69％，具有良好的工业应用前景。
附图说明
图1为酿酒酵母中虾青素合成路径示意图。
图2为实施例1构建的不同拷贝CrtW和CrtZ组合片段示意图。
具体实施方式
下述实施例中所使用的实验方法如无特殊说明，均为常规方法。
下述实施例中所用的材料、试剂等，如无特殊说明，均可从商业途径得到。
以下结合具体实施例，对本发明作进一步说明。应理解，以下实施例仅用于说明本发明而非用于限定本发明的范围。
酿酒酵母S228C(Saccharomyces cerevisiae S228C)在文献“Fisk etal.Saccharomyces cerevisiae S288C genome annotation:a workinghypothesis.Yeast.2006；23(12):857–865”中公开过，公众可从万华化学集团股份有限公司获得。
PRS426为武汉淼灵生物科技有限公司产品，货号为P0459。
D-(+)-半乳糖为阿拉丁产品，货号为G100367。
实施例中的CrtZ基因为橙黄农杆菌(Agrobacterium aurantiacum)来源的CrtZ，CrtW基因为泡囊短波单胞菌(Brevundimonas vesicularis DC263)来源的CrtW。
实施例1：以产β-胡萝卜素酿酒酵母为底盘菌株构建产高纯度虾青素的工程菌株
一、构建CrtW和CrtZ的基因表达片段
(一)以启动子TDH3p(SEQ ID NO:1)、基因CrtW(SEQ ID NO:2)、终止子TDH2t(SEQID NO:3)为模板,采用OE-PCR的方法构建TDH3p-CrtW-TDH2t片段，TDH3p-CrtW-TDH2t片段的序列如SEQ ID NO:10所示。
OE-PCR体系(50μl反应体系)如下：
启动子TDH3p、基因CrtW和终止子TDH2t模板各1μl、上游引物各1μl、下游引物各1μl、10×Buffer 5μl、dNTP 4μl，Pfu DNA Polymerase 1μl，加入ddH2O补足体积至50μl。
所用引物如下：
Primer 1-F：5’-cagttcgagtttatcattatcaatactgcca-3’(SEQ ID NO:4)；
Primer 1-R：5’-tcattggagtaacagcggacattttgtttgtttatgtgtgtttattcg-3’(SEQID NO:5)；
Primer 2-F：5’-cgaataaacacacataaacaaacaaaatgtccgctgttactccaatg-3’(SEQID NO:6)；
Primer 2-R：5’-aagtaacttaaggagttaaatttatgaaaataaagaccaccaagg-3’(SEQ IDNO:7)；
Primer 3-F：5’-tggtggtctttattttcataaatttaactccttaagttactttaatg-3’(SEQID NO:8)；
Primer 3-R：5’-gcgaaaagccaattagtgtgatac-3’(SEQ ID NO:9)。
(二)以启动子FBA1p(SEQ ID NO:11)、基因CrtW、终止子TEF2t(SEQ ID NO:12)为模板，采用OE-PCR的方法构建FBA1p-CrtW-TEF2t片段，FBA1p-CrtW-TEF2t片段的序列如SEQID NO:19所示。
OE-PCR体系(50μl反应体系)如下：
启动子FBA1p、基因CrtW和终止子TEF2t模板各1μl、上游引物各1μl、下游引物各1μl、10×Buffer 5μl、dNTP 4μl，Pfu DNA Polymerase 1μl，加入ddH2O补足体积至50μl。
所用引物如下：
Primer 4-F：5’-tccaactggcaccgctggcttgaacaaca-3’(SEQ ID NO:13)；
Primer 4-R：5’-tggagtaacagcggacattttgaatatgtattacttggtt-3’(SEQ ID NO:14)；
Primer 5-F：5’-aaccaagtaatacatattcaaaatgtccgctgttact-3’(SEQ ID NO:15)；
Primer 5-R：5’-ggaagcaataattattactctttatgaaaataaagacc-3’(SEQ ID NO:16)；
Primer 6-F：5’-ctttattttcataaagagtaataattattgcttccatataa-3’(SEQ ID NO:17)；
Primer 6-R：5’-acgctgacatggtttctttaggtt-3’(SEQ ID NO:18)。
(三)以启动子HXT7p(SEQ ID NO:20)、基因CrtW、终止子CYC1t(SEQ ID NO:21)为模板，采用OE-PCR的方法构建HXT7p-CrtW-CYC1t片段，HXT7p-CrtW-CYC1t片段的序列如SEQID NO:28所示。
OE-PCR体系(50μl反应体系)如下：
启动子HXT7p、基因CrtW和终止子CYC1t模板各1μl、上游引物各1μl、下游引物各1μl、10×Buffer 5μl、dNTP 4μl，Pfu DNA Polymerase 1μl，加入ddH2O补足体积至50μl。
所用引物如下：
Primer 7-F：5’-tctagtttctgccttaaacaaagccgc-3’(SEQ ID NO:22)；
Primer 7-R：5’-tggagtaacagcggacatttttttgattaaaattaaaaaaa-3’(SEQ ID NO:23)；
Primer 8-F：5’-tttttaattttaatcaaaaaaatgtccgctgttactccaatgagtaga-3’(SEQID NO:24)；
Primer 8-R：5’-aaggaaaaggggcctgttttatgaaaataaagacca-3’(SEQ ID NO:25)；
Primer 9-F：5’-gtggtctttattttcataaaacaggccccttttcc-3’(SEQ ID NO:26)；
Primer 9-R：5’-agagtgtaaactgcgaagcttgcaaattaa-3’(SEQ ID NO:27)。
(四)以启动子Gal1(SEQ ID NO:29)、基因CrtZ(SEQ ID NO:30)、终止子ADH1t(SEQID NO:31)为模板，采用OE-PCR的方法构建Gal1-CrtZ-ADH1t片段，Gal1-CrtZ-ADH1t片段的序列如SEQ ID NO:38所示。
OE-PCR体系(50μl反应体系)如下：
启动子Gal1、基因CrtZ和终止子ADH1t模板各1μl、上游引物各1μl、下游引物各1μl、10×Buffer 5μl、dNTP 4μl，Pfu DNA Polymerase 1μl，加入ddH2O补足体积至50μl。
所用引物如下：
Primer 10-F：5’-tatagttttttctccttgacgttaaagtatagaggtatattaacaa-3’(SEQID NO:32)；
Primer 10-R：5’-caagaagttagtcatttatattgaattttcaaaaattcttact-3’(SEQ IDNO:33)；
Primer 11-F：5’-aagaatttttgaaaattcaatataaatgactaacttcttgatcgttg-3’(SEQID NO:34)；
Primer 11-R：5’-cgaagaagtccaaagctttaagttctttcttgag-3’(SEQ ID NO:35)；
Primer 12-F：5’-caagaaagaacttaaagctttggacttcttcgccagag-3’(SEQ ID NO:36)；
Primer 12-R：5’-catgccggtagaggtgtggtcaataag-3’(SEQ ID NO:37)。
(五)以启动子Gal10(SEQ ID NO:39)、基因CrtZ、终止子TPI1t(SEQ ID NO:40)为模板，采用OE-PCR的方法构建Gal10-CrtZ-TPI1t片段，Gal10-CrtZ-TPI1t片段的序列如SEQID NO:47所示。
OE-PCR体系(50μl反应体系)如下：
启动子Gal10、基因CrtZ和终止子TPI1t模板各1μl、上游引物各1μl、下游引物各1μl、10×Buffer 5μl、dNTP 4μl，Pfu DNA Polymerase 1μl，加入ddH2O补足体积至50μl。
所用引物如下：
Primer 13-F：5’-ttatattgaattttcaaaaattcttactttttttttggatggacgc-3’(SEQID NO:41)；
Primer 13-R：5’-acaacgatcaagaagttagtcattatagttttttctccttgacg-3’(SEQ IDNO:42)；
Primer 14-F：5’-caaggagaaaaaactataatgactaacttcttgatcgttgttgctac-3’(SEQID NO:43)；
Primer 14-R：5’-atataattatattaatctttaagttctttcttgagcttcagctctc-3’(SEQID NO:44)；
Primer 15-F：5’-gaagctcaagaaagaacttaaagattaatataattatataaaaatattatcttcttttc-3’(SEQ ID NO:45)；
Primer 15-R：5’-ttaaatgcttttcttctttttattagaaaaagcgccttgc-3’(SEQ ID NO:46)。
二、组装不同拷贝的CrtW和CrtZ基因
(一)以TDH3p-CrtW-TDH2t片段、FBA1p-CrtW-TEF2t片段、Gal1-CrtZ-ADH1t片段为模板，采用OE-PCR的方法，组装CrtW基因和CrtZ基因拷贝数之比为2:1的TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t片段，TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t片段的序列如SEQ ID NO:54所示。
OE-PCR体系(50μl反应体系)如下：
TDH3p-CrtW-TDH2t片段、FBA1p-CrtW-TEF2t片段和Gal1-CrtZ-ADH1t片段模板各1μl、上游引物各1μl、下游引物各1μl、10×Buffer 5μl、dNTP 4μl，Pfu DNA Polymerase 1μl，加入ddH2O补足体积至50μl。
所用引物如下：
Primer 16-F：5’-cagttcgagtttatcattatcaatactgccatttc-3’(SEQ ID NO:48)；
Primer 16-R：5’-gtgccagttggagcgaaaagccaattagtgtg-3’(SEQ ID NO:49)；
Primer 17-F：5’-cactaattggcttttcgctccaactggcac-3’(SEQ ID NO:50)；
Primer 17-R：5’-gagaaaaaactataacgctgacatggtttctttaggtttgatgag-3’(SEQID NO:51)；
Primer 18-F：5’-aaccatgtcagcgttatagttttttctccttgacgtt-3’(SEQ ID NO:52)；
Primer 18-R：5’-catgccggtagaggtgtggtcaataagagc-3’(SEQ ID NO:53)。
以TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t片段为模板，以两边带有SalΙ和BamHΙ酶切位点的Primer 19-F和Primer 19-R为引物进行PCR扩增，获得BamHΙ-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-SalΙ，BamHΙ-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-SalΙ的序列如SEQ ID NO:57所示。
Primer 19-F：5’-ggatcccagttcgagtttatcattatc-3’(SEQ ID NO:55)；
Primer 19-R：5’-gtcgaccatgccggtagag-3’(SEQ ID NO:56)。
用SalΙ和BamHΙ双酶切BamHΙ-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-SalΙ片段，得到基因片段；用SalΙ和BamHΙ双酶切PRS426质粒，得到载体片段；将该基因片段和该载体片段连接，获得PRS426-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t重组质粒，序列如SEQ ID NO:58所示。
(二)以TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t、HXT7p-CrtW-CYC1t为模板，采用OE-PCR的方法，组装CrtW基因和CrtZ基因拷贝数之比为3:1的HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t片段，序列如SEQ IDNO:63所示。
OE-PCR体系(50μl反应体系)如下：
TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t、HXT7p-CrtW-CYC1t模板各1μl、上游引物各1μl、下游引物各1μl、10×Buffer 5μl、dNTP 4μl，Pfu DNAPolymerase1μl，加入ddH2O补足体积至50μl。
所用引物如下：
Primer 20-F：5’-tctagtttctgccttaaacaaagccgcag-3’(SEQ ID NO:59)；
Primer 20-R：5’-ataatgataaactcgaactgagagtgtaaactgc-3’(SEQ ID NO:60)；
Primer 21-F：5’-cagtttacactctcagttcgagtttatcattatcaatac-3’(SEQ ID NO:61)；
Primer 21-R：5’-catgccggtagaggtgtggtcaataagag-3’(SEQ ID NO:62)。
以HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t片段为模板，以两边带有SalΙ和BamHΙ酶切位点的Primer 22-F和Primer 22-R为引物进行PCR扩增，获得BamHΙ-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-SalΙ，序列如SEQ ID NO:66所示。
Primer 22-F：5’-ggatcctctagtttctgccttaaacaaagccgc-3’(SEQ ID NO:64)；
Primer 22-R：5’-gtcgaccatgccggtagaggtgtggtcaataag-3’(SEQ ID NO:65)。
SalΙ和BamHΙ双酶切BamHΙ-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-SalΙ片段，得到基因片段；SalΙ和BamHΙ双酶切PRS426质粒，得到载体片段；将该基因片段和载体片段连接，获得PRS426-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t重组质粒，序列如SEQ ID NO:67所示。
(三)
以HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t、Gal10-CrtZ-TPI1t为模板，经过OE-PCR组装获得CrtW基因和CrtZ基因拷贝数为3:2的BamHΙ-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-Gal10-CrtZ-TPI1t-SalΙ片段，序列如SEQ ID NO:72所示。
OE-PCR体系(50μl反应体系)如下：
HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t、Gal10-CrtZ-TPI1t模板各1μl、上游引物各1μl、下游引物各1μl、10×Buffer 5μl、dNTP 4μl，Pfu DNA Polymerase 1μl，加入ddH2O补足体积至50μl。
所用引物如下：
Primer 23-F：5’-ggatcctctagtttctgccttaaacaaagcc-3’(SEQ ID NO:68)；
Primer 23-R：5’-tgaaaattcaatataacatgccggtagaggtgtggtc-3’(SEQ ID NO:69)；
Primer 24-F：5’-cctctaccggcatgttatattgaattttcaaaaattcttac-3’(SEQ IDNO:70)；
Primer 24-R：5’-gtcgacttaaatgcttttcttctttttattagaaaaagcgc-3’(SEQ IDNO:71)。
SalΙ和BamHΙ双酶切BamHΙ-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-Gal10-CrtZ-TPI1t-SalΙ，得到基因片段；SalΙ和BamHΙ双酶切PRS426质粒，得到载体片段；将该基因片段和载体片段连接，获得PRS426-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-Gal10-CrtZ-TPI1t重组质粒，序列如SEQ ID NO:73所示。
将以上获得的重组质粒导入大肠杆菌，经转化子筛选和基因测序，确认重组质粒序列正确。
不同拷贝CrtW和CrtZ组合片段示意图如图2所示。
(四)将重组质粒PRS426-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t、PRS426-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t、PRS426-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-Gal10-CrtZ-TPI1t运用醋酸锂转化法分别转化生产β-胡萝卜素的Scy10026酿酒酵母(Saccharomyces cerevisiae)，获得生产虾青素的酿酒酵母工程菌株S7、S8、S9。
上述Scy10026酿酒酵母的构建方法如下：将CYC1t-BtCrtI-HXT7p-TDH3p-PaCrtB-ADH1t片段(SEQ ID NO:74)转入酿酒酵母S288C，同源重组并替换掉gal1、gal7、gal10基因，整合到染色体上；再将CYC1t-PaCrtY-HXT7p-FBA1p-TmCrtE-TDH2t片段(SEQ ID NO:75)转入其中，同源重组并替换掉ypl062w基因，整合到染色体上，获得生产β-胡萝卜素的酵母Scy10026。
CYC1t-BtCrtI-HXT7p-TDH3p-PaCrtB-ADH1t(SEQ ID NO:74)中：
第1-40位为gal 7基因下游同源序列，第41-241位为终止子CYC1t序列，第242-1990位为基因BtCrtI序列，第1991-2691位为启动子HXT7p序列，第2692-3371位为启动子TDH3p序列，第3372-4301位为基因PaCrtB序列，第4302-4629位为终止子ADH1t序列，第4630-4669位为gal1基因下游同源序列。
CYC1t-PaCrtY-HXT7p-FBA1p-TmCrtE-TDH2t(SEQ ID NO:75)中：
第1-40位为ypl062w基因上游同源序列，第41-241位为终止子CYC1t序列，第242-1390位为PaCrtY基因序列，第1391-2091位为启动子HXT7p序列，第2092-2911位为启动子FBA1p序列，第2912-4093位为基因TmCrtE序列，第4094-4493位为终止子TDH2t序列，第4494-4533位为ypl062w基因下游同源序列。
实施例2：摇瓶发酵生产虾青素
将实施例1中构建的生产虾青素的酿酒酵母工程菌株S7、S8、S9分别接种到5mlSC-Ura液体培养基中，于30℃、250rpm过夜培养得到一级种子，随后按1-2％(v/v)的接种量将一级种子接种到新鲜的5ml SC-Ura液体培养基中，于30℃、250rpm进行二级种子培养至OD600＝5-6，得到二级种子。转接二级种子接种到装有50mL含4％葡萄糖的YPD培养基(10g/L酵母膏、20g/L蛋白胨、40g/L葡萄糖，余量为水)的250ml摇瓶中发酵培养，初始OD600＝0.1，温度30℃，转速250rpm发酵42小时加入终浓度2％(2g/100ml)的D-(+)-半乳糖诱导虾青素生成，发酵84小时结束，得到发酵液。
然后，取适量发酵液，并对其中的酵母工程菌株进行破壁处理，再用丙酮提取虾青素，然后上样进行虾青素HPLC高效液相色谱检测。
虾青素HPLC高效液相色谱检测条件如下：
采用双波长检测，测定波长设为450/470nm，积分波长470nm；
柱温：25℃；
流动相：流速为1mL/min，流动相B：乙腈:水＝9:1(体积比)，流动相C：甲醇:异丙醇＝3:2(体积比)，洗脱条件为：0-15min，0-90％C；15-30min，90％C；30-35min，90-0％C；35-45min，0％C。外标法计算虾青素浓度。
按照下式计算虾青素纯度：
W＝Ca/C*100％
W为纯度，Ca为虾青素浓度(mg/L)，C为类胡萝卜素浓度(mg/L)；
其中类胡萝卜素浓度同样采用HPLC外标法检测得到，HPLC检测条件与上述虾青素HPLC高效液相色谱检测条件一致。
经检测，不同酿酒酵母工程菌株合成虾青素的产量和纯度如表1所示。
表1不同酿酒酵母工程菌株合成虾青素的产量和纯度
Figure BDA0002500308910000091
表1显示，经过调整CrtW与CrtZ之间的相对拷贝数，虾青素的产量和纯度均出现了显著变化，当CrtW:CrtZ拷贝数之比为3:2时，虾青素产量达到了11.17mg/g DCW，纯度达到了91.79％。
实施例3：摇瓶发酵过程中不同的诱导剂加入时间对虾青素产量和纯度的影响
将生产虾青素的工程菌株S9按照实施例2中的种子培养方法培养获得二级种子，然后将其接入五组装有50mL含4％葡萄糖的YPD培养基的250ml摇瓶(每组设置两个平行)Y1、Y2、Y3、Y4、Y5中进行发酵培养，初始OD600＝0.1，温度30℃，转速250rpm，分别在发酵的第30h、36h、42h、48h、54h加入终浓度2％的D-(+)-半乳糖诱导虾青素生成，发酵84小时结束，得到发酵液。然后，取适量发酵液，并对其中的酵母工程菌株进行破壁处理，再用丙酮提取虾青素，然后上样进行虾青素HPLC高效液相色谱检测(检测条件与实施例2中的相同)，各组的虾青素产量和纯度如表2所示。
表2发酵过程中不同的诱导剂加入时间下的虾青素产量和纯度
Figure BDA0002500308910000101
表2显示，诱导剂的加入时机能够较大地影响虾青素的产量和纯度，在发酵的第36小时加入终浓度2％的D-(+)-半乳糖，虾青素的产量和纯度均达到最高，分别为12.84mg/gDCW和93.33％。
实施例4：不同发酵温度对虾青素产量和纯度的影响
将生产虾青素的工程菌株S9按照实施例2中的种子培养方法培养获得二级种子，然后将其接入三组装有50mL含4％葡萄糖的YPD培养基的250ml摇瓶(每组设置两个平行)T1、T2、T3中进行发酵培养，初始OD600＝0.1，转速250rpm，其中T1发酵温度设置为20℃，T2发酵温度设置为25℃，T3发酵温度设置为30℃，三组摇瓶均在发酵的第36小时添加终浓度2％的D-(+)-半乳糖诱导虾青素生成，发酵84小时结束，得到发酵液。同样地，取适量发酵液，并对其中的酵母工程菌株进行破壁处理，再用丙酮提取虾青素，然后上样进行虾青素HPLC高效液相色谱检测(检测条件与实施例2中的相同)，各组的虾青素产量和纯度如表3所示。
表3发酵过程中不同温度下的虾青素产量和纯度
Figure BDA0002500308910000102
Figure BDA0002500308910000111
表3表明，当发酵温度设置为20℃，最有利于虾青素的生产，虾青素的产量和纯度分别达到14.70mg/g DCW和96.69％。
序列表
<110> 万华化学集团股份有限公司
万华化学（四川）有限公司
<120> 一株生产虾青素的重组酿酒酵母及其应用
<130> DSP1F200499ZX
<160> 75
<170> SIPOSequenceListing 1.0
<210> 1
<211> 680
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
cagttcgagt ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt 60
agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt 120
acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga 180
acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag 240
aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt 300
ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac 360
ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc 420
atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa 480
agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt 540
atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat 600
tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata 660
aacacacata aacaaacaaa 680
<210> 2
<211> 726
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 2
atgtccgctg ttactccaat gagtagagtt gtccctaatc aagcattgat tggtttgact 60
ttagcaggtt tgattgctgc tgcttggttg acattgcata tatacggtgt ctacttccac 120
agatggacaa tctggtctgt tttgaccgtc ccattaattg tagctggtca aacttggttg 180
tccgtaggtt tattcatagt tgctcatgat gcaatgcacg gtagtttggc accagccaga 240
cctagattaa acacagcaat cggttctttg gctttagcat tgtatgccgg ttttagattc 300
accccattga aaactgccca tcacgctcat cacgctgcac caggtacagc agatgaccct 360
gattttcatg ccgacgctcc aagagcattt ttgccttggt tctatggttt ctttagaacc 420
tacttcggtt ggagagaatt agcagttttg actgtattag ttgcagtcgc cgtattaata 480
ttgggtgcta gaatgccaaa tttgttagtc ttttgggccg ctcctgcatt gttgtctgcc 540
ttacaattgt tcactttcgg tacatggttg ccacatagac acaccgatga cgcctttcct 600
gataatcata acgctagaac atctccattc ggtcctgttt tatcattgtt gacctgtttc 660
catttcggta gacatcacga acatcacttg acaccttgga agccttggtg gtctttattt 720
tcataa 726
<210> 3
<211> 400
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
atttaactcc ttaagttact ttaatgattt agtttttatt attaataatt catgctcatg 60
acatctcata tacacgttta taaaacttaa atagattgaa aatgtattaa agattcctca 120
gggattcgat ttttttggaa gtttttgttt ttttttcctt gagatgctgt agtatttggg 180
aacaattata caatcgaaag atatatgctt acattcgacc gttttagccg tgatcattat 240
cctatagtaa cataacctga agcataactg acactactat catcaatact tgtcacatga 300
gaactctgtg aataattagg ccactgaaat ttgatgcctg aaggaccggc atcacggatt 360
ttcgataaag cacttagtat cacactaatt ggcttttcgc 400
<210> 4
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
cagttcgagt ttatcattat caatactgcc a 31
<210> 5
<211> 48
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 5
tcattggagt aacagcggac attttgtttg tttatgtgtg tttattcg 48
<210> 6
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 6
cgaataaaca cacataaaca aacaaaatgt ccgctgttac tccaatg 47
<210> 7
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
aagtaactta aggagttaaa tttatgaaaa taaagaccac caagg 45
<210> 8
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
tggtggtctt tattttcata aatttaactc cttaagttac tttaatg 47
<210> 9
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 9
gcgaaaagcc aattagtgtg atac 24
<210> 10
<211> 1806
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 10
cagttcgagt ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt 60
agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt 120
acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga 180
acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag 240
aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt 300
ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac 360
ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc 420
atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa 480
agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt 540
atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat 600
tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata 660
aacacacata aacaaacaaa atgtccgctg ttactccaat gagtagagtt gtccctaatc 720
aagcattgat tggtttgact ttagcaggtt tgattgctgc tgcttggttg acattgcata 780
tatacggtgt ctacttccac agatggacaa tctggtctgt tttgaccgtc ccattaattg 840
tagctggtca aacttggttg tccgtaggtt tattcatagt tgctcatgat gcaatgcacg 900
gtagtttggc accagccaga cctagattaa acacagcaat cggttctttg gctttagcat 960
tgtatgccgg ttttagattc accccattga aaactgccca tcacgctcat cacgctgcac 1020
caggtacagc agatgaccct gattttcatg ccgacgctcc aagagcattt ttgccttggt 1080
tctatggttt ctttagaacc tacttcggtt ggagagaatt agcagttttg actgtattag 1140
ttgcagtcgc cgtattaata ttgggtgcta gaatgccaaa tttgttagtc ttttgggccg 1200
ctcctgcatt gttgtctgcc ttacaattgt tcactttcgg tacatggttg ccacatagac 1260
acaccgatga cgcctttcct gataatcata acgctagaac atctccattc ggtcctgttt 1320
tatcattgtt gacctgtttc catttcggta gacatcacga acatcacttg acaccttgga 1380
agccttggtg gtctttattt tcataaattt aactccttaa gttactttaa tgatttagtt 1440
tttattatta ataattcatg ctcatgacat ctcatataca cgtttataaa acttaaatag 1500
attgaaaatg tattaaagat tcctcaggga ttcgattttt ttggaagttt ttgttttttt 1560
ttccttgaga tgctgtagta tttgggaaca attatacaat cgaaagatat atgcttacat 1620
tcgaccgttt tagccgtgat cattatccta tagtaacata acctgaagca taactgacac 1680
tactatcatc aatacttgtc acatgagaac tctgtgaata attaggccac tgaaatttga 1740
tgcctgaagg accggcatca cggattttcg ataaagcact tagtatcaca ctaattggct 1800
tttcgc 1806
<210> 11
<211> 820
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 11
tccaactggc accgctggct tgaacaacaa taccagcctt ccaacttctg taaataacgg 60
cggtacgcca gtgccaccag taccgttacc tttcggtata cctcctttcc ccatgtttcc 120
aatgcccttc atgcctccaa cggctactat cacaaatcct catcaagctg acgcaagccc 180
taagaaatga ataacaatac tgacagtact aaataattgc ctacttggct tcacatacgt 240
tgcatacgtc gatatagata ataatgataa tgacagcagg attatcgtaa tacgtaatag 300
ttgaaaatct caaaaatgtg tgggtcatta cgtaaataat gataggaatg ggattcttct 360
atttttcctt tttccattct agcagccgtc gggaaaacgt ggcatcctct ctttcgggct 420
caattggagt cacgctgccg tgagcatcct ctctttccat atctaacaac tgagcacgta 480
accaatggaa aagcatgagc ttagcgttgc tccaaaaaag tattggatgg ttaataccat 540
ttgtctgttc tcttctgact ttgactcctc aaaaaaaaaa aatctacaat caacagatcg 600
cttcaattac gccctcacaa aaactttttt ccttcttctt cgcccacgtt aaattttatc 660
cctcatgttg tctaacggat ttctgcactt gatttattat aaaaagacaa agacataata 720
cttctctatc aatttcagtt attgttcttc cttgcgttat tcttctgttc ttctttttct 780
tttgtcatat ataaccataa ccaagtaata catattcaaa 820
<210> 12
<211> 301
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 12
agagtaataa ttattgcttc catataatat ttttatatac ctcttatttt tatgtattag 60
ttaattaagt atttttatct atctgcttat cattttcttt tcatataggg ggggttggtg 120
ttttcttgcc catcagattg atgtcctcca actcggcact attttacaaa gggttttttt 180
gtaagagaag gagaagacag atactaaacc atacgttact cgaaacaaaa aaaaaaaaaa 240
tggaaaaagc tgctatcaac aaaagacggc ctcatcaaac ctaaagaaac catgtcagcg 300
t 301
<210> 13
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 13
tccaactggc accgctggct tgaacaaca 29
<210> 14
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 14
tggagtaaca gcggacattt tgaatatgta ttacttggtt 40
<210> 15
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 15
aaccaagtaa tacatattca aaatgtccgc tgttact 37
<210> 16
<211> 38
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 16
ggaagcaata attattactc tttatgaaaa taaagacc 38
<210> 17
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 17
ctttattttc ataaagagta ataattattg cttccatata a 41
<210> 18
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 18
acgctgacat ggtttcttta ggtt 24
<210> 19
<211> 1847
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 19
tccaactggc accgctggct tgaacaacaa taccagcctt ccaacttctg taaataacgg 60
cggtacgcca gtgccaccag taccgttacc tttcggtata cctcctttcc ccatgtttcc 120
aatgcccttc atgcctccaa cggctactat cacaaatcct catcaagctg acgcaagccc 180
taagaaatga ataacaatac tgacagtact aaataattgc ctacttggct tcacatacgt 240
tgcatacgtc gatatagata ataatgataa tgacagcagg attatcgtaa tacgtaatag 300
ttgaaaatct caaaaatgtg tgggtcatta cgtaaataat gataggaatg ggattcttct 360
atttttcctt tttccattct agcagccgtc gggaaaacgt ggcatcctct ctttcgggct 420
caattggagt cacgctgccg tgagcatcct ctctttccat atctaacaac tgagcacgta 480
accaatggaa aagcatgagc ttagcgttgc tccaaaaaag tattggatgg ttaataccat 540
ttgtctgttc tcttctgact ttgactcctc aaaaaaaaaa aatctacaat caacagatcg 600
cttcaattac gccctcacaa aaactttttt ccttcttctt cgcccacgtt aaattttatc 660
cctcatgttg tctaacggat ttctgcactt gatttattat aaaaagacaa agacataata 720
cttctctatc aatttcagtt attgttcttc cttgcgttat tcttctgttc ttctttttct 780
tttgtcatat ataaccataa ccaagtaata catattcaaa atgtccgctg ttactccaat 840
gagtagagtt gtccctaatc aagcattgat tggtttgact ttagcaggtt tgattgctgc 900
tgcttggttg acattgcata tatacggtgt ctacttccac agatggacaa tctggtctgt 960
tttgaccgtc ccattaattg tagctggtca aacttggttg tccgtaggtt tattcatagt 1020
tgctcatgat gcaatgcacg gtagtttggc accagccaga cctagattaa acacagcaat 1080
cggttctttg gctttagcat tgtatgccgg ttttagattc accccattga aaactgccca 1140
tcacgctcat cacgctgcac caggtacagc agatgaccct gattttcatg ccgacgctcc 1200
aagagcattt ttgccttggt tctatggttt ctttagaacc tacttcggtt ggagagaatt 1260
agcagttttg actgtattag ttgcagtcgc cgtattaata ttgggtgcta gaatgccaaa 1320
tttgttagtc ttttgggccg ctcctgcatt gttgtctgcc ttacaattgt tcactttcgg 1380
tacatggttg ccacatagac acaccgatga cgcctttcct gataatcata acgctagaac 1440
atctccattc ggtcctgttt tatcattgtt gacctgtttc catttcggta gacatcacga 1500
acatcacttg acaccttgga agccttggtg gtctttattt tcataaagag taataattat 1560
tgcttccata taatattttt atatacctct tatttttatg tattagttaa ttaagtattt 1620
ttatctatct gcttatcatt ttcttttcat ataggggggg ttggtgtttt cttgcccatc 1680
agattgatgt cctccaactc ggcactattt tacaaagggt ttttttgtaa gagaaggaga 1740
agacagatac taaaccatac gttactcgaa acaaaaaaaa aaaaaatgga aaaagctgct 1800
atcaacaaaa gacggcctca tcaaacctaa agaaaccatg tcagcgt 1847
<210> 20
<211> 701
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 20
tctagtttct gccttaaaca aagccgcagc cagagccgtt tttccgccat atttatccag 60
gattgttcca tacggctccg tcagaggctg ctacgggatg tttttttttt accccgtgga 120
aatgaggggt atgcaggaat ttgtgcgggg taggaaatct tttttttttt taggaggaac 180
aactggtgga agaatgccca cacttctcag aaatgcatgc agtggcagca cgctaattcg 240
aaaaaattct ccagaaaggc aacgcaaaat tttttttcca gggaataaac tttttatgac 300
ccactacttc tcgtaggaac aatttcgggc ccctgcgtgt tcttctgagg ttcatctttt 360
acatttgctt ctgctggata attttcagag gcaacaagga aaaattagat ggcaaaaagt 420
cgtctttcaa ggaaaaatcc ccaccatctt tcgagatccc ctgtaactta ttggcaactg 480
aaagaatgaa aaggaggaaa atacaaaata tactagaact gaaaaaaaaa aagtataaat 540
agagacgata tatgccaata cttcacaatg ttcgaatcta ttcttcattt gcagctattg 600
taaaataata aaacatcaag aacaaacaag ctcaacttgt cttttctaag aacaaagaat 660
aaacacaaaa acaaaaagtt tttttaattt taatcaaaaa a 701
<210> 21
<211> 297
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 21
aacaggcccc ttttcctttg tcgatatcat gtaattagtt atgtcacgct tacattcacg 60
ccctcctccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt 120
ccctatttat tttttttaat agttatgtta gtattaagaa cgttatttat atttcaaatt 180
tttctttttt ttctgtacaa acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt 240
gagaaggttt tgggacgctc gaaggcttta atttgcaagc ttcgcagttt acactct 297
<210> 22
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 22
tctagtttct gccttaaaca aagccgc 27
<210> 23
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 23
tggagtaaca gcggacattt ttttgattaa aattaaaaaa a 41
<210> 24
<211> 48
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 24
tttttaattt taatcaaaaa aatgtccgct gttactccaa tgagtaga 48
<210> 25
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 25
aaggaaaagg ggcctgtttt atgaaaataa agacca 36
<210> 26
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 26
gtggtcttta ttttcataaa acaggcccct tttcc 35
<210> 27
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 27
agagtgtaaa ctgcgaagct tgcaaattaa 30
<210> 28
<211> 1724
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 28
tctagtttct gccttaaaca aagccgcagc cagagccgtt tttccgccat atttatccag 60
gattgttcca tacggctccg tcagaggctg ctacgggatg tttttttttt accccgtgga 120
aatgaggggt atgcaggaat ttgtgcgggg taggaaatct tttttttttt taggaggaac 180
aactggtgga agaatgccca cacttctcag aaatgcatgc agtggcagca cgctaattcg 240
aaaaaattct ccagaaaggc aacgcaaaat tttttttcca gggaataaac tttttatgac 300
ccactacttc tcgtaggaac aatttcgggc ccctgcgtgt tcttctgagg ttcatctttt 360
acatttgctt ctgctggata attttcagag gcaacaagga aaaattagat ggcaaaaagt 420
cgtctttcaa ggaaaaatcc ccaccatctt tcgagatccc ctgtaactta ttggcaactg 480
aaagaatgaa aaggaggaaa atacaaaata tactagaact gaaaaaaaaa aagtataaat 540
agagacgata tatgccaata cttcacaatg ttcgaatcta ttcttcattt gcagctattg 600
taaaataata aaacatcaag aacaaacaag ctcaacttgt cttttctaag aacaaagaat 660
aaacacaaaa acaaaaagtt tttttaattt taatcaaaaa aatgtccgct gttactccaa 720
tgagtagagt tgtccctaat caagcattga ttggtttgac tttagcaggt ttgattgctg 780
ctgcttggtt gacattgcat atatacggtg tctacttcca cagatggaca atctggtctg 840
ttttgaccgt cccattaatt gtagctggtc aaacttggtt gtccgtaggt ttattcatag 900
ttgctcatga tgcaatgcac ggtagtttgg caccagccag acctagatta aacacagcaa 960
tcggttcttt ggctttagca ttgtatgccg gttttagatt caccccattg aaaactgccc 1020
atcacgctca tcacgctgca ccaggtacag cagatgaccc tgattttcat gccgacgctc 1080
caagagcatt tttgccttgg ttctatggtt tctttagaac ctacttcggt tggagagaat 1140
tagcagtttt gactgtatta gttgcagtcg ccgtattaat attgggtgct agaatgccaa 1200
atttgttagt cttttgggcc gctcctgcat tgttgtctgc cttacaattg ttcactttcg 1260
gtacatggtt gccacataga cacaccgatg acgcctttcc tgataatcat aacgctagaa 1320
catctccatt cggtcctgtt ttatcattgt tgacctgttt ccatttcggt agacatcacg 1380
aacatcactt gacaccttgg aagccttggt ggtctttatt ttcataaaac aggccccttt 1440
tcctttgtcg atatcatgta attagttatg tcacgcttac attcacgccc tcctcccaca 1500
tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt 1560
ttttaatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc 1620
tgtacaaacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg 1680
gacgctcgaa ggctttaatt tgcaagcttc gcagtttaca ctct 1724
<210> 29
<211> 668
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 29
tatagttttt tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac 60
ttttatgaca tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag 120
tggttatgca gcttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg 180
attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tattatccta tggttgttaa 240
tttgattcgt tgatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata 300
accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat 360
ctgcgtttca ggaacgcgac cggtgaagac caggacgcac ggaggagagt cttccgtcgg 420
agggctgtcg cccgctcggc ggcttctaat ccgtacttca atatagcaat gagcagttaa 480
gcgtattact gaaagttcca aagagaaggt ttttttaggc taagataatg gggctcttta 540
catttccaca acatataagt aagattagat atggatatgt atatggtggt attgccatgt 600
aatatgatta ttaaacttct ttgcgtccat ccaaaaaaaa agtaagaatt tttgaaaatt 660
caatataa 668
<210> 30
<211> 489
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 30
atgactaact tcttgatcgt tgttgctact gttttggtta tggaattgac tgcttactct 60
gttcacagat ggatcatgca cggtccattg ggttggggtt ggcacaagtc tcaccacgaa 120
gaacacgacc acgctttgga aaagaacgac ttgtacggtt tggttttcgc tgttatcgct 180
actgttttgt tcactgttgg ttggatctgg gctccagttt tgtggtggat cgctttgggt 240
atgactgttt acggtttgat ctacttcgtt ttgcacgacg gtttggttca ccaaagatgg 300
ccattcagat acatcccaag aaagggttac gctagaagat tgtaccaagc tcacagattg 360
caccacgctg ttgaaggtag agaccactgt gtttctttcg gtttcatcta cgctccacca 420
gttgacaagt tgaagcaaga cttgaagatg tctggtgttt tgagagctga agctcaagaa 480
agaacttaa 489
<210> 31
<211> 328
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 31
agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg tcggcttgtc 60
taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta gatacgttgt 120
tgacacttct aaataagcga atttcttatg atttatgatt tttattatta aataagttat 180
aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt 240
attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc 300
tcttattgac cacacctcta ccggcatg 328
<210> 32
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 32
tatagttttt tctccttgac gttaaagtat agaggtatat taacaa 46
<210> 33
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 33
caagaagtta gtcatttata ttgaattttc aaaaattctt act 43
<210> 34
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 34
aagaattttt gaaaattcaa tataaatgac taacttcttg atcgttg 47
<210> 35
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 35
cgaagaagtc caaagcttta agttctttct tgag 34
<210> 36
<211> 38
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 36
caagaaagaa cttaaagctt tggacttctt cgccagag 38
<210> 37
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 37
catgccggta gaggtgtggt caataag 27
<210> 38
<211> 1485
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 38
tatagttttt tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac 60
ttttatgaca tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag 120
tggttatgca gcttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg 180
attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tattatccta tggttgttaa 240
tttgattcgt tgatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata 300
accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat 360
ctgcgtttca ggaacgcgac cggtgaagac caggacgcac ggaggagagt cttccgtcgg 420
agggctgtcg cccgctcggc ggcttctaat ccgtacttca atatagcaat gagcagttaa 480
gcgtattact gaaagttcca aagagaaggt ttttttaggc taagataatg gggctcttta 540
catttccaca acatataagt aagattagat atggatatgt atatggtggt attgccatgt 600
aatatgatta ttaaacttct ttgcgtccat ccaaaaaaaa agtaagaatt tttgaaaatt 660
caatataaat gactaacttc ttgatcgttg ttgctactgt tttggttatg gaattgactg 720
cttactctgt tcacagatgg atcatgcacg gtccattggg ttggggttgg cacaagtctc 780
accacgaaga acacgaccac gctttggaaa agaacgactt gtacggtttg gttttcgctg 840
ttatcgctac tgttttgttc actgttggtt ggatctgggc tccagttttg tggtggatcg 900
ctttgggtat gactgtttac ggtttgatct acttcgtttt gcacgacggt ttggttcacc 960
aaagatggcc attcagatac atcccaagaa agggttacgc tagaagattg taccaagctc 1020
acagattgca ccacgctgtt gaaggtagag accactgtgt ttctttcggt ttcatctacg 1080
ctccaccagt tgacaagttg aagcaagact tgaagatgtc tggtgttttg agagctgaag 1140
ctcaagaaag aacttaaagc tttggacttc ttcgccagag gtttggtcaa gtctccaatc 1200
aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa gggtcaaatc 1260
gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt tatgattttt 1320
attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt 1380
ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag 1440
gtatagcatg aggtcgctct tattgaccac acctctaccg gcatg 1485
<210> 39
<211> 668
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 39
ttatattgaa ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag aagtttaata 60
atcatattac atggcaatac caccatatac atatccatat ctaatcttac ttatatgttg 120
tggaaatgta aagagcccca ttatcttagc ctaaaaaaac cttctctttg gaactttcag 180
taatacgctt aactgctcat tgctatattg aagtacggat tagaagccgc cgagcgggcg 240
acagccctcc gacggaagac tctcctccgt gcgtcctggt cttcaccggt cgcgttcctg 300
aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct 360
tttatggtta tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaatcaac 420
gaatcaaatt aacaaccata ggataataat gcgattagtt ttttagcctt atttctgggg 480
taattaatca gcgaagcgat gatttttgat ctattaacag atatataaat gcaaaagctg 540
cataaccact ttaactaata ctttcaacat tttcggtttg tattacttct tattcaaatg 600
tcataaaagt atcaacaaaa aattgttaat atacctctat actttaacgt caaggagaaa 660
aaactata 668
<210> 40
<211> 215
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 40
agattaatat aattatataa aaatattatc ttcttttctt tatatctagt gttatgtaaa 60
ataaattgat gactacggaa agctttttta tattgtttct ttttcattct gagccactta 120
aatttcgtga atgttcttgt aagggacggt agatttacaa gtgatacaac aaaaagcaag 180
gcgctttttc taataaaaag aagaaaagca tttaa 215
<210> 41
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 41
ttatattgaa ttttcaaaaa ttcttacttt ttttttggat ggacgc 46
<210> 42
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 42
acaacgatca agaagttagt cattatagtt ttttctcctt gacg 44
<210> 43
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 43
caaggagaaa aaactataat gactaacttc ttgatcgttg ttgctac 47
<210> 44
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 44
atataattat attaatcttt aagttctttc ttgagcttca gctctc 46
<210> 45
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 45
gaagctcaag aaagaactta aagattaata taattatata aaaatattat cttcttttc 59
<210> 46
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 46
ttaaatgctt ttcttctttt tattagaaaa agcgccttgc 40
<210> 47
<211> 1372
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 47
ttatattgaa ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag aagtttaata 60
atcatattac atggcaatac caccatatac atatccatat ctaatcttac ttatatgttg 120
tggaaatgta aagagcccca ttatcttagc ctaaaaaaac cttctctttg gaactttcag 180
taatacgctt aactgctcat tgctatattg aagtacggat tagaagccgc cgagcgggcg 240
acagccctcc gacggaagac tctcctccgt gcgtcctggt cttcaccggt cgcgttcctg 300
aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct 360
tttatggtta tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaatcaac 420
gaatcaaatt aacaaccata ggataataat gcgattagtt ttttagcctt atttctgggg 480
taattaatca gcgaagcgat gatttttgat ctattaacag atatataaat gcaaaagctg 540
cataaccact ttaactaata ctttcaacat tttcggtttg tattacttct tattcaaatg 600
tcataaaagt atcaacaaaa aattgttaat atacctctat actttaacgt caaggagaaa 660
aaactataat gactaacttc ttgatcgttg ttgctactgt tttggttatg gaattgactg 720
cttactctgt tcacagatgg atcatgcacg gtccattggg ttggggttgg cacaagtctc 780
accacgaaga acacgaccac gctttggaaa agaacgactt gtacggtttg gttttcgctg 840
ttatcgctac tgttttgttc actgttggtt ggatctgggc tccagttttg tggtggatcg 900
ctttgggtat gactgtttac ggtttgatct acttcgtttt gcacgacggt ttggttcacc 960
aaagatggcc attcagatac atcccaagaa agggttacgc tagaagattg taccaagctc 1020
acagattgca ccacgctgtt gaaggtagag accactgtgt ttctttcggt ttcatctacg 1080
ctccaccagt tgacaagttg aagcaagact tgaagatgtc tggtgttttg agagctgaag 1140
ctcaagaaag aacttaaaga ttaatataat tatataaaaa tattatcttc ttttctttat 1200
atctagtgtt atgtaaaata aattgatgac tacggaaagc ttttttatat tgtttctttt 1260
tcattctgag ccacttaaat ttcgtgaatg ttcttgtaag ggacggtaga tttacaagtg 1320
atacaacaaa aagcaaggcg ctttttctaa taaaaagaag aaaagcattt aa 1372
<210> 48
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 48
cagttcgagt ttatcattat caatactgcc atttc 35
<210> 49
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 49
gtgccagttg gagcgaaaag ccaattagtg tg 32
<210> 50
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 50
cactaattgg cttttcgctc caactggcac 30
<210> 51
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 51
gagaaaaaac tataacgctg acatggtttc tttaggtttg atgag 45
<210> 52
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 52
aaccatgtca gcgttatagt tttttctcct tgacgtt 37
<210> 53
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 53
catgccggta gaggtgtggt caataagagc 30
<210> 54
<211> 5138
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 54
cagttcgagt ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt 60
agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt 120
acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga 180
acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag 240
aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt 300
ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac 360
ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc 420
atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa 480
agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt 540
atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat 600
tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata 660
aacacacata aacaaacaaa atgtccgctg ttactccaat gagtagagtt gtccctaatc 720
aagcattgat tggtttgact ttagcaggtt tgattgctgc tgcttggttg acattgcata 780
tatacggtgt ctacttccac agatggacaa tctggtctgt tttgaccgtc ccattaattg 840
tagctggtca aacttggttg tccgtaggtt tattcatagt tgctcatgat gcaatgcacg 900
gtagtttggc accagccaga cctagattaa acacagcaat cggttctttg gctttagcat 960
tgtatgccgg ttttagattc accccattga aaactgccca tcacgctcat cacgctgcac 1020
caggtacagc agatgaccct gattttcatg ccgacgctcc aagagcattt ttgccttggt 1080
tctatggttt ctttagaacc tacttcggtt ggagagaatt agcagttttg actgtattag 1140
ttgcagtcgc cgtattaata ttgggtgcta gaatgccaaa tttgttagtc ttttgggccg 1200
ctcctgcatt gttgtctgcc ttacaattgt tcactttcgg tacatggttg ccacatagac 1260
acaccgatga cgcctttcct gataatcata acgctagaac atctccattc ggtcctgttt 1320
tatcattgtt gacctgtttc catttcggta gacatcacga acatcacttg acaccttgga 1380
agccttggtg gtctttattt tcataaattt aactccttaa gttactttaa tgatttagtt 1440
tttattatta ataattcatg ctcatgacat ctcatataca cgtttataaa acttaaatag 1500
attgaaaatg tattaaagat tcctcaggga ttcgattttt ttggaagttt ttgttttttt 1560
ttccttgaga tgctgtagta tttgggaaca attatacaat cgaaagatat atgcttacat 1620
tcgaccgttt tagccgtgat cattatccta tagtaacata acctgaagca taactgacac 1680
tactatcatc aatacttgtc acatgagaac tctgtgaata attaggccac tgaaatttga 1740
tgcctgaagg accggcatca cggattttcg ataaagcact tagtatcaca ctaattggct 1800
tttcgctcca actggcaccg ctggcttgaa caacaatacc agccttccaa cttctgtaaa 1860
taacggcggt acgccagtgc caccagtacc gttacctttc ggtatacctc ctttccccat 1920
gtttccaatg cccttcatgc ctccaacggc tactatcaca aatcctcatc aagctgacgc 1980
aagccctaag aaatgaataa caatactgac agtactaaat aattgcctac ttggcttcac 2040
atacgttgca tacgtcgata tagataataa tgataatgac agcaggatta tcgtaatacg 2100
taatagttga aaatctcaaa aatgtgtggg tcattacgta aataatgata ggaatgggat 2160
tcttctattt ttcctttttc cattctagca gccgtcggga aaacgtggca tcctctcttt 2220
cgggctcaat tggagtcacg ctgccgtgag catcctctct ttccatatct aacaactgag 2280
cacgtaacca atggaaaagc atgagcttag cgttgctcca aaaaagtatt ggatggttaa 2340
taccatttgt ctgttctctt ctgactttga ctcctcaaaa aaaaaaaatc tacaatcaac 2400
agatcgcttc aattacgccc tcacaaaaac ttttttcctt cttcttcgcc cacgttaaat 2460
tttatccctc atgttgtcta acggatttct gcacttgatt tattataaaa agacaaagac 2520
ataatacttc tctatcaatt tcagttattg ttcttccttg cgttattctt ctgttcttct 2580
ttttcttttg tcatatataa ccataaccaa gtaatacata ttcaaaatgt ccgctgttac 2640
tccaatgagt agagttgtcc ctaatcaagc attgattggt ttgactttag caggtttgat 2700
tgctgctgct tggttgacat tgcatatata cggtgtctac ttccacagat ggacaatctg 2760
gtctgttttg accgtcccat taattgtagc tggtcaaact tggttgtccg taggtttatt 2820
catagttgct catgatgcaa tgcacggtag tttggcacca gccagaccta gattaaacac 2880
agcaatcggt tctttggctt tagcattgta tgccggtttt agattcaccc cattgaaaac 2940
tgcccatcac gctcatcacg ctgcaccagg tacagcagat gaccctgatt ttcatgccga 3000
cgctccaaga gcatttttgc cttggttcta tggtttcttt agaacctact tcggttggag 3060
agaattagca gttttgactg tattagttgc agtcgccgta ttaatattgg gtgctagaat 3120
gccaaatttg ttagtctttt gggccgctcc tgcattgttg tctgccttac aattgttcac 3180
tttcggtaca tggttgccac atagacacac cgatgacgcc tttcctgata atcataacgc 3240
tagaacatct ccattcggtc ctgttttatc attgttgacc tgtttccatt tcggtagaca 3300
tcacgaacat cacttgacac cttggaagcc ttggtggtct ttattttcat aaagagtaat 3360
aattattgct tccatataat atttttatat acctcttatt tttatgtatt agttaattaa 3420
gtatttttat ctatctgctt atcattttct tttcatatag ggggggttgg tgttttcttg 3480
cccatcagat tgatgtcctc caactcggca ctattttaca aagggttttt ttgtaagaga 3540
aggagaagac agatactaaa ccatacgtta ctcgaaacaa aaaaaaaaaa aatggaaaaa 3600
gctgctatca acaaaagacg gcctcatcaa acctaaagaa accatgtcag cgttatagtt 3660
ttttctcctt gacgttaaag tatagaggta tattaacaat tttttgttga tacttttatg 3720
acatttgaat aagaagtaat acaaaccgaa aatgttgaaa gtattagtta aagtggttat 3780
gcagcttttg catttatata tctgttaata gatcaaaaat catcgcttcg ctgattaatt 3840
accccagaaa taaggctaaa aaactaatcg cattattatc ctatggttgt taatttgatt 3900
cgttgatttg aaggtttgtg gggccaggtt actgccaatt tttcctcttc ataaccataa 3960
aagctagtat tgtagaatct ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt 4020
tcaggaacgc gaccggtgaa gaccaggacg cacggaggag agtcttccgt cggagggctg 4080
tcgcccgctc ggcggcttct aatccgtact tcaatatagc aatgagcagt taagcgtatt 4140
actgaaagtt ccaaagagaa ggttttttta ggctaagata atggggctct ttacatttcc 4200
acaacatata agtaagatta gatatggata tgtatatggt ggtattgcca tgtaatatga 4260
ttattaaact tctttgcgtc catccaaaaa aaaagtaaga atttttgaaa attcaatata 4320
aatgactaac ttcttgatcg ttgttgctac tgttttggtt atggaattga ctgcttactc 4380
tgttcacaga tggatcatgc acggtccatt gggttggggt tggcacaagt ctcaccacga 4440
agaacacgac cacgctttgg aaaagaacga cttgtacggt ttggttttcg ctgttatcgc 4500
tactgttttg ttcactgttg gttggatctg ggctccagtt ttgtggtgga tcgctttggg 4560
tatgactgtt tacggtttga tctacttcgt tttgcacgac ggtttggttc accaaagatg 4620
gccattcaga tacatcccaa gaaagggtta cgctagaaga ttgtaccaag ctcacagatt 4680
gcaccacgct gttgaaggta gagaccactg tgtttctttc ggtttcatct acgctccacc 4740
agttgacaag ttgaagcaag acttgaagat gtctggtgtt ttgagagctg aagctcaaga 4800
aagaacttaa agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg 4860
tcggcttgtc taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta 4920
gatacgttgt tgacacttct aaataagcga atttcttatg atttatgatt tttattatta 4980
aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac 5040
gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc 5100
atgaggtcgc tcttattgac cacacctcta ccggcatg 5138
<210> 55
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 55
ggatcccagt tcgagtttat cattatc 27
<210> 56
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 56
gtcgaccatg ccggtagag 19
<210> 57
<211> 5150
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 57
ggatcccagt tcgagtttat cattatcaat actgccattt caaagaatac gtaaataatt 60
aatagtagtg attttcctaa ctttatttag tcaaaaaatt agccttttaa ttctgctgta 120
acccgtacat gcccaaaata gggggcgggt tacacagaat atataacatc gtaggtgtct 180
gggtgaacag tttattcctg gcatccacta aatataatgg agcccgcttt ttaagctggc 240
atccagaaaa aaaaagaatc ccagcaccaa aatattgttt tcttcaccaa ccatcagttc 300
ataggtccat tctcttagcg caactacaga gaacaggggc acaaacaggc aaaaaacggg 360
cacaacctca atggagtgat gcaacctgcc tggagtaaat gatgacacaa ggcaattgac 420
ccacgcatgt atctatctca ttttcttaca ccttctatta ccttctgctc tctctgattt 480
ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa attattcccc tacttgacta 540
ataagtatat aaagacggta ggtattgatt gtaattctgt aaatctattt cttaaacttc 600
ttaaattcta cttttatagt tagtcttttt tttagtttta aaacaccaag aacttagttt 660
cgaataaaca cacataaaca aacaaaatgt ccgctgttac tccaatgagt agagttgtcc 720
ctaatcaagc attgattggt ttgactttag caggtttgat tgctgctgct tggttgacat 780
tgcatatata cggtgtctac ttccacagat ggacaatctg gtctgttttg accgtcccat 840
taattgtagc tggtcaaact tggttgtccg taggtttatt catagttgct catgatgcaa 900
tgcacggtag tttggcacca gccagaccta gattaaacac agcaatcggt tctttggctt 960
tagcattgta tgccggtttt agattcaccc cattgaaaac tgcccatcac gctcatcacg 1020
ctgcaccagg tacagcagat gaccctgatt ttcatgccga cgctccaaga gcatttttgc 1080
cttggttcta tggtttcttt agaacctact tcggttggag agaattagca gttttgactg 1140
tattagttgc agtcgccgta ttaatattgg gtgctagaat gccaaatttg ttagtctttt 1200
gggccgctcc tgcattgttg tctgccttac aattgttcac tttcggtaca tggttgccac 1260
atagacacac cgatgacgcc tttcctgata atcataacgc tagaacatct ccattcggtc 1320
ctgttttatc attgttgacc tgtttccatt tcggtagaca tcacgaacat cacttgacac 1380
cttggaagcc ttggtggtct ttattttcat aaatttaact ccttaagtta ctttaatgat 1440
ttagttttta ttattaataa ttcatgctca tgacatctca tatacacgtt tataaaactt 1500
aaatagattg aaaatgtatt aaagattcct cagggattcg atttttttgg aagtttttgt 1560
ttttttttcc ttgagatgct gtagtatttg ggaacaatta tacaatcgaa agatatatgc 1620
ttacattcga ccgttttagc cgtgatcatt atcctatagt aacataacct gaagcataac 1680
tgacactact atcatcaata cttgtcacat gagaactctg tgaataatta ggccactgaa 1740
atttgatgcc tgaaggaccg gcatcacgga ttttcgataa agcacttagt atcacactaa 1800
ttggcttttc gctccaactg gcaccgctgg cttgaacaac aataccagcc ttccaacttc 1860
tgtaaataac ggcggtacgc cagtgccacc agtaccgtta cctttcggta tacctccttt 1920
ccccatgttt ccaatgccct tcatgcctcc aacggctact atcacaaatc ctcatcaagc 1980
tgacgcaagc cctaagaaat gaataacaat actgacagta ctaaataatt gcctacttgg 2040
cttcacatac gttgcatacg tcgatataga taataatgat aatgacagca ggattatcgt 2100
aatacgtaat agttgaaaat ctcaaaaatg tgtgggtcat tacgtaaata atgataggaa 2160
tgggattctt ctatttttcc tttttccatt ctagcagccg tcgggaaaac gtggcatcct 2220
ctctttcggg ctcaattgga gtcacgctgc cgtgagcatc ctctctttcc atatctaaca 2280
actgagcacg taaccaatgg aaaagcatga gcttagcgtt gctccaaaaa agtattggat 2340
ggttaatacc atttgtctgt tctcttctga ctttgactcc tcaaaaaaaa aaaatctaca 2400
atcaacagat cgcttcaatt acgccctcac aaaaactttt ttccttcttc ttcgcccacg 2460
ttaaatttta tccctcatgt tgtctaacgg atttctgcac ttgatttatt ataaaaagac 2520
aaagacataa tacttctcta tcaatttcag ttattgttct tccttgcgtt attcttctgt 2580
tcttcttttt cttttgtcat atataaccat aaccaagtaa tacatattca aaatgtccgc 2640
tgttactcca atgagtagag ttgtccctaa tcaagcattg attggtttga ctttagcagg 2700
tttgattgct gctgcttggt tgacattgca tatatacggt gtctacttcc acagatggac 2760
aatctggtct gttttgaccg tcccattaat tgtagctggt caaacttggt tgtccgtagg 2820
tttattcata gttgctcatg atgcaatgca cggtagtttg gcaccagcca gacctagatt 2880
aaacacagca atcggttctt tggctttagc attgtatgcc ggttttagat tcaccccatt 2940
gaaaactgcc catcacgctc atcacgctgc accaggtaca gcagatgacc ctgattttca 3000
tgccgacgct ccaagagcat ttttgccttg gttctatggt ttctttagaa cctacttcgg 3060
ttggagagaa ttagcagttt tgactgtatt agttgcagtc gccgtattaa tattgggtgc 3120
tagaatgcca aatttgttag tcttttgggc cgctcctgca ttgttgtctg ccttacaatt 3180
gttcactttc ggtacatggt tgccacatag acacaccgat gacgcctttc ctgataatca 3240
taacgctaga acatctccat tcggtcctgt tttatcattg ttgacctgtt tccatttcgg 3300
tagacatcac gaacatcact tgacaccttg gaagccttgg tggtctttat tttcataaag 3360
agtaataatt attgcttcca tataatattt ttatatacct cttattttta tgtattagtt 3420
aattaagtat ttttatctat ctgcttatca ttttcttttc atataggggg ggttggtgtt 3480
ttcttgccca tcagattgat gtcctccaac tcggcactat tttacaaagg gtttttttgt 3540
aagagaagga gaagacagat actaaaccat acgttactcg aaacaaaaaa aaaaaaaatg 3600
gaaaaagctg ctatcaacaa aagacggcct catcaaacct aaagaaacca tgtcagcgtt 3660
atagtttttt ctccttgacg ttaaagtata gaggtatatt aacaattttt tgttgatact 3720
tttatgacat ttgaataaga agtaatacaa accgaaaatg ttgaaagtat tagttaaagt 3780
ggttatgcag cttttgcatt tatatatctg ttaatagatc aaaaatcatc gcttcgctga 3840
ttaattaccc cagaaataag gctaaaaaac taatcgcatt attatcctat ggttgttaat 3900
ttgattcgtt gatttgaagg tttgtggggc caggttactg ccaatttttc ctcttcataa 3960
ccataaaagc tagtattgta gaatctttat tgttcggagc agtgcggcgc gaggcacatc 4020
tgcgtttcag gaacgcgacc ggtgaagacc aggacgcacg gaggagagtc ttccgtcgga 4080
gggctgtcgc ccgctcggcg gcttctaatc cgtacttcaa tatagcaatg agcagttaag 4140
cgtattactg aaagttccaa agagaaggtt tttttaggct aagataatgg ggctctttac 4200
atttccacaa catataagta agattagata tggatatgta tatggtggta ttgccatgta 4260
atatgattat taaacttctt tgcgtccatc caaaaaaaaa gtaagaattt ttgaaaattc 4320
aatataaatg actaacttct tgatcgttgt tgctactgtt ttggttatgg aattgactgc 4380
ttactctgtt cacagatgga tcatgcacgg tccattgggt tggggttggc acaagtctca 4440
ccacgaagaa cacgaccacg ctttggaaaa gaacgacttg tacggtttgg ttttcgctgt 4500
tatcgctact gttttgttca ctgttggttg gatctgggct ccagttttgt ggtggatcgc 4560
tttgggtatg actgtttacg gtttgatcta cttcgttttg cacgacggtt tggttcacca 4620
aagatggcca ttcagataca tcccaagaaa gggttacgct agaagattgt accaagctca 4680
cagattgcac cacgctgttg aaggtagaga ccactgtgtt tctttcggtt tcatctacgc 4740
tccaccagtt gacaagttga agcaagactt gaagatgtct ggtgttttga gagctgaagc 4800
tcaagaaaga acttaaagct ttggacttct tcgccagagg tttggtcaag tctccaatca 4860
aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg 4920
ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta 4980
ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt 5040
taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg 5100
tatagcatga ggtcgctctt attgaccaca cctctaccgg catggtcgac 5150
<210> 58
<211> 10825
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 58
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 240
ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 300
agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 360
cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 420
cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 480
ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 540
aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 600
tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 660
ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 720
aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 780
acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 840
aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 900
gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 960
ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 1020
ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 1080
atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 1140
gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 1200
gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 1260
aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 1320
agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 1380
tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 1440
tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 1500
agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 1560
gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 1620
aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 1680
cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 1740
gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 1800
gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 1860
cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 1920
taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat 1980
acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac catgccggta 2040
gaggtgtggt caataagagc gacctcatgc tatacctgag aaagcaacct gacctacagg 2100
aaagagttac tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg 2160
tatacactta ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc 2220
gcttatttag aagtgtcaac aacgtatcta ccaacgattt gacccttttc catcttttcg 2280
taaatttctg gcaaggtaga caagccgaca accttgattg gagacttgac caaacctctg 2340
gcgaagaagt ccaaagcttt aagttctttc ttgagcttca gctctcaaaa caccagacat 2400
cttcaagtct tgcttcaact tgtcaactgg tggagcgtag atgaaaccga aagaaacaca 2460
gtggtctcta ccttcaacag cgtggtgcaa tctgtgagct tggtacaatc ttctagcgta 2520
accctttctt gggatgtatc tgaatggcca tctttggtga accaaaccgt cgtgcaaaac 2580
gaagtagatc aaaccgtaaa cagtcatacc caaagcgatc caccacaaaa ctggagccca 2640
gatccaacca acagtgaaca aaacagtagc gataacagcg aaaaccaaac cgtacaagtc 2700
gttcttttcc aaagcgtggt cgtgttcttc gtggtgagac ttgtgccaac cccaacccaa 2760
tggaccgtgc atgatccatc tgtgaacaga gtaagcagtc aattccataa ccaaaacagt 2820
agcaacaacg atcaagaagt tagtcattta tattgaattt tcaaaaattc ttactttttt 2880
tttggatgga cgcaaagaag tttaataatc atattacatg gcaataccac catatacata 2940
tccatatcta atcttactta tatgttgtgg aaatgtaaag agccccatta tcttagccta 3000
aaaaaacctt ctctttggaa ctttcagtaa tacgcttaac tgctcattgc tatattgaag 3060
tacggattag aagccgccga gcgggcgaca gccctccgac ggaagactct cctccgtgcg 3120
tcctggtctt caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg 3180
aacaataaag attctacaat actagctttt atggttatga agaggaaaaa ttggcagtaa 3240
cctggcccca caaaccttca aatcaacgaa tcaaattaac aaccatagga taataatgcg 3300
attagttttt tagccttatt tctggggtaa ttaatcagcg aagcgatgat ttttgatcta 3360
ttaacagata tataaatgca aaagctgcat aaccacttta actaatactt tcaacatttt 3420
cggtttgtat tacttcttat tcaaatgtca taaaagtatc aacaaaaaat tgttaatata 3480
cctctatact ttaacgtcaa ggagaaaaaa ctataacgct gacatggttt ctttaggttt 3540
gatgaggccg tcttttgttg atagcagctt tttccatttt tttttttttt gtttcgagta 3600
acgtatggtt tagtatctgt cttctccttc tcttacaaaa aaaccctttg taaaatagtg 3660
ccgagttgga ggacatcaat ctgatgggca agaaaacacc aaccccccct atatgaaaag 3720
aaaatgataa gcagatagat aaaaatactt aattaactaa tacataaaaa taagaggtat 3780
ataaaaatat tatatggaag caataattat tactctttat gaaaataaag accaccaagg 3840
cttccaaggt gtcaagtgat gttcgtgatg tctaccgaaa tggaaacagg tcaacaatga 3900
taaaacagga ccgaatggag atgttctagc gttatgatta tcaggaaagg cgtcatcggt 3960
gtgtctatgt ggcaaccatg taccgaaagt gaacaattgt aaggcagaca acaatgcagg 4020
agcggcccaa aagactaaca aatttggcat tctagcaccc aatattaata cggcgactgc 4080
aactaataca gtcaaaactg ctaattctct ccaaccgaag taggttctaa agaaaccata 4140
gaaccaaggc aaaaatgctc ttggagcgtc ggcatgaaaa tcagggtcat ctgctgtacc 4200
tggtgcagcg tgatgagcgt gatgggcagt tttcaatggg gtgaatctaa aaccggcata 4260
caatgctaaa gccaaagaac cgattgctgt gtttaatcta ggtctggctg gtgccaaact 4320
accgtgcatt gcatcatgag caactatgaa taaacctacg gacaaccaag tttgaccagc 4380
tacaattaat gggacggtca aaacagacca gattgtccat ctgtggaagt agacaccgta 4440
tatatgcaat gtcaaccaag cagcagcaat caaacctgct aaagtcaaac caatcaatgc 4500
ttgattaggg acaactctac tcattggagt aacagcggac attttgaata tgtattactt 4560
ggttatggtt atatatgaca aaagaaaaag aagaacagaa gaataacgca aggaagaaca 4620
ataactgaaa ttgatagaga agtattatgt ctttgtcttt ttataataaa tcaagtgcag 4680
aaatccgtta gacaacatga gggataaaat ttaacgtggg cgaagaagaa ggaaaaaagt 4740
ttttgtgagg gcgtaattga agcgatctgt tgattgtaga tttttttttt ttgaggagtc 4800
aaagtcagaa gagaacagac aaatggtatt aaccatccaa tacttttttg gagcaacgct 4860
aagctcatgc ttttccattg gttacgtgct cagttgttag atatggaaag agaggatgct 4920
cacggcagcg tgactccaat tgagcccgaa agagaggatg ccacgttttc ccgacggctg 4980
ctagaatgga aaaaggaaaa atagaagaat cccattccta tcattattta cgtaatgacc 5040
cacacatttt tgagattttc aactattacg tattacgata atcctgctgt cattatcatt 5100
attatctata tcgacgtatg caacgtatgt gaagccaagt aggcaattat ttagtactgt 5160
cagtattgtt attcatttct tagggcttgc gtcagcttga tgaggatttg tgatagtagc 5220
cgttggaggc atgaagggca ttggaaacat ggggaaagga ggtataccga aaggtaacgg 5280
tactggtggc actggcgtac cgccgttatt tacagaagtt ggaaggctgg tattgttgtt 5340
caagccagcg gtgccagttg gagcgaaaag ccaattagtg tgatactaag tgctttatcg 5400
aaaatccgtg atgccggtcc ttcaggcatc aaatttcagt ggcctaatta ttcacagagt 5460
tctcatgtga caagtattga tgatagtagt gtcagttatg cttcaggtta tgttactata 5520
ggataatgat cacggctaaa acggtcgaat gtaagcatat atctttcgat tgtataattg 5580
ttcccaaata ctacagcatc tcaaggaaaa aaaaacaaaa acttccaaaa aaatcgaatc 5640
cctgaggaat ctttaataca ttttcaatct atttaagttt tataaacgtg tatatgagat 5700
gtcatgagca tgaattatta ataataaaaa ctaaatcatt aaagtaactt aaggagttaa 5760
atttatgaaa ataaagacca ccaaggcttc caaggtgtca agtgatgttc gtgatgtcta 5820
ccgaaatgga aacaggtcaa caatgataaa acaggaccga atggagatgt tctagcgtta 5880
tgattatcag gaaaggcgtc atcggtgtgt ctatgtggca accatgtacc gaaagtgaac 5940
aattgtaagg cagacaacaa tgcaggagcg gcccaaaaga ctaacaaatt tggcattcta 6000
gcacccaata ttaatacggc gactgcaact aatacagtca aaactgctaa ttctctccaa 6060
ccgaagtagg ttctaaagaa accatagaac caaggcaaaa atgctcttgg agcgtcggca 6120
tgaaaatcag ggtcatctgc tgtacctggt gcagcgtgat gagcgtgatg ggcagttttc 6180
aatggggtga atctaaaacc ggcatacaat gctaaagcca aagaaccgat tgctgtgttt 6240
aatctaggtc tggctggtgc caaactaccg tgcattgcat catgagcaac tatgaataaa 6300
cctacggaca accaagtttg accagctaca attaatggga cggtcaaaac agaccagatt 6360
gtccatctgt ggaagtagac accgtatata tgcaatgtca accaagcagc agcaatcaaa 6420
cctgctaaag tcaaaccaat caatgcttga ttagggacaa ctctactcat tggagtaaca 6480
gcggacattt tgtttgttta tgtgtgttta ttcgaaacta agttcttggt gttttaaaac 6540
taaaaaaaag actaactata aaagtagaat ttaagaagtt taagaaatag atttacagaa 6600
ttacaatcaa tacctaccgt ctttatatac ttattagtca agtaggggaa taatttcagg 6660
gaactggttt caaccttttt tttcagcttt ttccaaatca gagagagcag aaggtaatag 6720
aaggtgtaag aaaatgagat agatacatgc gtgggtcaat tgccttgtgt catcatttac 6780
tccaggcagg ttgcatcact ccattgaggt tgtgcccgtt ttttgcctgt ttgtgcccct 6840
gttctctgta gttgcgctaa gagaatggac ctatgaactg atggttggtg aagaaaacaa 6900
tattttggtg ctgggattct ttttttttct ggatgccagc ttaaaaagcg ggctccatta 6960
tatttagtgg atgccaggaa taaactgttc acccagacac ctacgatgtt atatattctg 7020
tgtaacccgc cccctatttt gggcatgtac gggttacagc agaattaaaa ggctaatttt 7080
ttgactaaat aaagttagga aaatcactac tattaattat ttacgtattc tttgaaatgg 7140
cagtattgat aatgataaac tcgaactggg atccactagt tctagagcgg ccgccaccgc 7200
ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat 7260
ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacataggag 7320
ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc acattaattg 7380
cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 7440
tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 7500
ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 7560
taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 7620
agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 7680
cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 7740
tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 7800
tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 7860
gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 7920
acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 7980
acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 8040
cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 8100
gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 8160
gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 8220
agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 8280
ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 8340
ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 8400
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 8460
tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 8520
gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 8580
ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 8640
caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 8700
cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 8760
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 8820
cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 8880
agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 8940
tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 9000
agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 9060
atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 9120
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 9180
cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 9240
caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 9300
attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 9360
agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaacgaa 9420
gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca 9480
aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa 9540
cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc 9600
aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt 9660
accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt 9720
tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct 9780
cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta 9840
ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag 9900
ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat 9960
tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt 10020
atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg 10080
tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa 10140
tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa 10200
aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt 10260
tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc 10320
gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa 10380
gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa 10440
acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt tcacgtcgca 10500
cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt 10560
tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc 10620
tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt 10680
agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt 10740
tcctttgata ttggatcata ctaagaaacc attattatca tgacattaac ctataaaaat 10800
aggcgtatca cgaggccctt tcgtc 10825
<210> 59
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 59
tctagtttct gccttaaaca aagccgcag 29
<210> 60
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 60
ataatgataa actcgaactg agagtgtaaa ctgc 34
<210> 61
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 61
cagtttacac tctcagttcg agtttatcat tatcaatac 39
<210> 62
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 62
catgccggta gaggtgtggt caataagag 29
<210> 63
<211> 6862
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 63
tctagtttct gccttaaaca aagccgcagc cagagccgtt tttccgccat atttatccag 60
gattgttcca tacggctccg tcagaggctg ctacgggatg tttttttttt accccgtgga 120
aatgaggggt atgcaggaat ttgtgcgggg taggaaatct tttttttttt taggaggaac 180
aactggtgga agaatgccca cacttctcag aaatgcatgc agtggcagca cgctaattcg 240
aaaaaattct ccagaaaggc aacgcaaaat tttttttcca gggaataaac tttttatgac 300
ccactacttc tcgtaggaac aatttcgggc ccctgcgtgt tcttctgagg ttcatctttt 360
acatttgctt ctgctggata attttcagag gcaacaagga aaaattagat ggcaaaaagt 420
cgtctttcaa ggaaaaatcc ccaccatctt tcgagatccc ctgtaactta ttggcaactg 480
aaagaatgaa aaggaggaaa atacaaaata tactagaact gaaaaaaaaa aagtataaat 540
agagacgata tatgccaata cttcacaatg ttcgaatcta ttcttcattt gcagctattg 600
taaaataata aaacatcaag aacaaacaag ctcaacttgt cttttctaag aacaaagaat 660
aaacacaaaa acaaaaagtt tttttaattt taatcaaaaa aatgtccgct gttactccaa 720
tgagtagagt tgtccctaat caagcattga ttggtttgac tttagcaggt ttgattgctg 780
ctgcttggtt gacattgcat atatacggtg tctacttcca cagatggaca atctggtctg 840
ttttgaccgt cccattaatt gtagctggtc aaacttggtt gtccgtaggt ttattcatag 900
ttgctcatga tgcaatgcac ggtagtttgg caccagccag acctagatta aacacagcaa 960
tcggttcttt ggctttagca ttgtatgccg gttttagatt caccccattg aaaactgccc 1020
atcacgctca tcacgctgca ccaggtacag cagatgaccc tgattttcat gccgacgctc 1080
caagagcatt tttgccttgg ttctatggtt tctttagaac ctacttcggt tggagagaat 1140
tagcagtttt gactgtatta gttgcagtcg ccgtattaat attgggtgct agaatgccaa 1200
atttgttagt cttttgggcc gctcctgcat tgttgtctgc cttacaattg ttcactttcg 1260
gtacatggtt gccacataga cacaccgatg acgcctttcc tgataatcat aacgctagaa 1320
catctccatt cggtcctgtt ttatcattgt tgacctgttt ccatttcggt agacatcacg 1380
aacatcactt gacaccttgg aagccttggt ggtctttatt ttcataaaac aggccccttt 1440
tcctttgtcg atatcatgta attagttatg tcacgcttac attcacgccc tcctcccaca 1500
tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt 1560
ttttaatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc 1620
tgtacaaacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg 1680
gacgctcgaa ggctttaatt tgcaagcttc gcagtttaca ctctcagttc gagtttatca 1740
ttatcaatac tgccatttca aagaatacgt aaataattaa tagtagtgat tttcctaact 1800
ttatttagtc aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg 1860
gggcgggtta cacagaatat ataacatcgt aggtgtctgg gtgaacagtt tattcctggc 1920
atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa aaagaatccc 1980
agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc tcttagcgca 2040
actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat ggagtgatgc 2100
aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat ctatctcatt 2160
ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt 2220
tgaaaccagt tccctgaaat tattccccta cttgactaat aagtatataa agacggtagg 2280
tattgattgt aattctgtaa atctatttct taaacttctt aaattctact tttatagtta 2340
gtcttttttt tagttttaaa acaccaagaa cttagtttcg aataaacaca cataaacaaa 2400
caaaatgtcc gctgttactc caatgagtag agttgtccct aatcaagcat tgattggttt 2460
gactttagca ggtttgattg ctgctgcttg gttgacattg catatatacg gtgtctactt 2520
ccacagatgg acaatctggt ctgttttgac cgtcccatta attgtagctg gtcaaacttg 2580
gttgtccgta ggtttattca tagttgctca tgatgcaatg cacggtagtt tggcaccagc 2640
cagacctaga ttaaacacag caatcggttc tttggcttta gcattgtatg ccggttttag 2700
attcacccca ttgaaaactg cccatcacgc tcatcacgct gcaccaggta cagcagatga 2760
ccctgatttt catgccgacg ctccaagagc atttttgcct tggttctatg gtttctttag 2820
aacctacttc ggttggagag aattagcagt tttgactgta ttagttgcag tcgccgtatt 2880
aatattgggt gctagaatgc caaatttgtt agtcttttgg gccgctcctg cattgttgtc 2940
tgccttacaa ttgttcactt tcggtacatg gttgccacat agacacaccg atgacgcctt 3000
tcctgataat cataacgcta gaacatctcc attcggtcct gttttatcat tgttgacctg 3060
tttccatttc ggtagacatc acgaacatca cttgacacct tggaagcctt ggtggtcttt 3120
attttcataa atttaactcc ttaagttact ttaatgattt agtttttatt attaataatt 3180
catgctcatg acatctcata tacacgttta taaaacttaa atagattgaa aatgtattaa 3240
agattcctca gggattcgat ttttttggaa gtttttgttt ttttttcctt gagatgctgt 3300
agtatttggg aacaattata caatcgaaag atatatgctt acattcgacc gttttagccg 3360
tgatcattat cctatagtaa cataacctga agcataactg acactactat catcaatact 3420
tgtcacatga gaactctgtg aataattagg ccactgaaat ttgatgcctg aaggaccggc 3480
atcacggatt ttcgataaag cacttagtat cacactaatt ggcttttcgc tccaactggc 3540
accgctggct tgaacaacaa taccagcctt ccaacttctg taaataacgg cggtacgcca 3600
gtgccaccag taccgttacc tttcggtata cctcctttcc ccatgtttcc aatgcccttc 3660
atgcctccaa cggctactat cacaaatcct catcaagctg acgcaagccc taagaaatga 3720
ataacaatac tgacagtact aaataattgc ctacttggct tcacatacgt tgcatacgtc 3780
gatatagata ataatgataa tgacagcagg attatcgtaa tacgtaatag ttgaaaatct 3840
caaaaatgtg tgggtcatta cgtaaataat gataggaatg ggattcttct atttttcctt 3900
tttccattct agcagccgtc gggaaaacgt ggcatcctct ctttcgggct caattggagt 3960
cacgctgccg tgagcatcct ctctttccat atctaacaac tgagcacgta accaatggaa 4020
aagcatgagc ttagcgttgc tccaaaaaag tattggatgg ttaataccat ttgtctgttc 4080
tcttctgact ttgactcctc aaaaaaaaaa aatctacaat caacagatcg cttcaattac 4140
gccctcacaa aaactttttt ccttcttctt cgcccacgtt aaattttatc cctcatgttg 4200
tctaacggat ttctgcactt gatttattat aaaaagacaa agacataata cttctctatc 4260
aatttcagtt attgttcttc cttgcgttat tcttctgttc ttctttttct tttgtcatat 4320
ataaccataa ccaagtaata catattcaaa atgtccgctg ttactccaat gagtagagtt 4380
gtccctaatc aagcattgat tggtttgact ttagcaggtt tgattgctgc tgcttggttg 4440
acattgcata tatacggtgt ctacttccac agatggacaa tctggtctgt tttgaccgtc 4500
ccattaattg tagctggtca aacttggttg tccgtaggtt tattcatagt tgctcatgat 4560
gcaatgcacg gtagtttggc accagccaga cctagattaa acacagcaat cggttctttg 4620
gctttagcat tgtatgccgg ttttagattc accccattga aaactgccca tcacgctcat 4680
cacgctgcac caggtacagc agatgaccct gattttcatg ccgacgctcc aagagcattt 4740
ttgccttggt tctatggttt ctttagaacc tacttcggtt ggagagaatt agcagttttg 4800
actgtattag ttgcagtcgc cgtattaata ttgggtgcta gaatgccaaa tttgttagtc 4860
ttttgggccg ctcctgcatt gttgtctgcc ttacaattgt tcactttcgg tacatggttg 4920
ccacatagac acaccgatga cgcctttcct gataatcata acgctagaac atctccattc 4980
ggtcctgttt tatcattgtt gacctgtttc catttcggta gacatcacga acatcacttg 5040
acaccttgga agccttggtg gtctttattt tcataaagag taataattat tgcttccata 5100
taatattttt atatacctct tatttttatg tattagttaa ttaagtattt ttatctatct 5160
gcttatcatt ttcttttcat ataggggggg ttggtgtttt cttgcccatc agattgatgt 5220
cctccaactc ggcactattt tacaaagggt ttttttgtaa gagaaggaga agacagatac 5280
taaaccatac gttactcgaa acaaaaaaaa aaaaaatgga aaaagctgct atcaacaaaa 5340
gacggcctca tcaaacctaa agaaaccatg tcagcgttat agttttttct ccttgacgtt 5400
aaagtataga ggtatattaa caattttttg ttgatacttt tatgacattt gaataagaag 5460
taatacaaac cgaaaatgtt gaaagtatta gttaaagtgg ttatgcagct tttgcattta 5520
tatatctgtt aatagatcaa aaatcatcgc ttcgctgatt aattacccca gaaataaggc 5580
taaaaaacta atcgcattat tatcctatgg ttgttaattt gattcgttga tttgaaggtt 5640
tgtggggcca ggttactgcc aatttttcct cttcataacc ataaaagcta gtattgtaga 5700
atctttattg ttcggagcag tgcggcgcga ggcacatctg cgtttcagga acgcgaccgg 5760
tgaagaccag gacgcacgga ggagagtctt ccgtcggagg gctgtcgccc gctcggcggc 5820
ttctaatccg tacttcaata tagcaatgag cagttaagcg tattactgaa agttccaaag 5880
agaaggtttt tttaggctaa gataatgggg ctctttacat ttccacaaca tataagtaag 5940
attagatatg gatatgtata tggtggtatt gccatgtaat atgattatta aacttctttg 6000
cgtccatcca aaaaaaaagt aagaattttt gaaaattcaa tataaatgac taacttcttg 6060
atcgttgttg ctactgtttt ggttatggaa ttgactgctt actctgttca cagatggatc 6120
atgcacggtc cattgggttg gggttggcac aagtctcacc acgaagaaca cgaccacgct 6180
ttggaaaaga acgacttgta cggtttggtt ttcgctgtta tcgctactgt tttgttcact 6240
gttggttgga tctgggctcc agttttgtgg tggatcgctt tgggtatgac tgtttacggt 6300
ttgatctact tcgttttgca cgacggtttg gttcaccaaa gatggccatt cagatacatc 6360
ccaagaaagg gttacgctag aagattgtac caagctcaca gattgcacca cgctgttgaa 6420
ggtagagacc actgtgtttc tttcggtttc atctacgctc caccagttga caagttgaag 6480
caagacttga agatgtctgg tgttttgaga gctgaagctc aagaaagaac ttaaagcttt 6540
ggacttcttc gccagaggtt tggtcaagtc tccaatcaag gttgtcggct tgtctacctt 6600
gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac 6660
ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa 6720
aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt 6780
gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat 6840
tgaccacacc tctaccggca tg 6862
<210> 64
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 64
ggatcctcta gtttctgcct taaacaaagc cgc 33
<210> 65
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 65
gtcgaccatg ccggtagagg tgtggtcaat aag 33
<210> 66
<211> 6874
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 66
ggatcctcta gtttctgcct taaacaaagc cgcagccaga gccgtttttc cgccatattt 60
atccaggatt gttccatacg gctccgtcag aggctgctac gggatgtttt ttttttaccc 120
cgtggaaatg aggggtatgc aggaatttgt gcggggtagg aaatcttttt tttttttagg 180
aggaacaact ggtggaagaa tgcccacact tctcagaaat gcatgcagtg gcagcacgct 240
aattcgaaaa aattctccag aaaggcaacg caaaattttt tttccaggga ataaactttt 300
tatgacccac tacttctcgt aggaacaatt tcgggcccct gcgtgttctt ctgaggttca 360
tcttttacat ttgcttctgc tggataattt tcagaggcaa caaggaaaaa ttagatggca 420
aaaagtcgtc tttcaaggaa aaatccccac catctttcga gatcccctgt aacttattgg 480
caactgaaag aatgaaaagg aggaaaatac aaaatatact agaactgaaa aaaaaaaagt 540
ataaatagag acgatatatg ccaatacttc acaatgttcg aatctattct tcatttgcag 600
ctattgtaaa ataataaaac atcaagaaca aacaagctca acttgtcttt tctaagaaca 660
aagaataaac acaaaaacaa aaagtttttt taattttaat caaaaaaatg tccgctgtta 720
ctccaatgag tagagttgtc cctaatcaag cattgattgg tttgacttta gcaggtttga 780
ttgctgctgc ttggttgaca ttgcatatat acggtgtcta cttccacaga tggacaatct 840
ggtctgtttt gaccgtccca ttaattgtag ctggtcaaac ttggttgtcc gtaggtttat 900
tcatagttgc tcatgatgca atgcacggta gtttggcacc agccagacct agattaaaca 960
cagcaatcgg ttctttggct ttagcattgt atgccggttt tagattcacc ccattgaaaa 1020
ctgcccatca cgctcatcac gctgcaccag gtacagcaga tgaccctgat tttcatgccg 1080
acgctccaag agcatttttg ccttggttct atggtttctt tagaacctac ttcggttgga 1140
gagaattagc agttttgact gtattagttg cagtcgccgt attaatattg ggtgctagaa 1200
tgccaaattt gttagtcttt tgggccgctc ctgcattgtt gtctgcctta caattgttca 1260
ctttcggtac atggttgcca catagacaca ccgatgacgc ctttcctgat aatcataacg 1320
ctagaacatc tccattcggt cctgttttat cattgttgac ctgtttccat ttcggtagac 1380
atcacgaaca tcacttgaca ccttggaagc cttggtggtc tttattttca taaaacaggc 1440
cccttttcct ttgtcgatat catgtaatta gttatgtcac gcttacattc acgccctcct 1500
cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt 1560
tatttttttt aatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt 1620
tttttctgta caaacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg 1680
ttttgggacg ctcgaaggct ttaatttgca agcttcgcag tttacactct cagttcgagt 1740
ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt agtgattttc 1800
ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt acatgcccaa 1860
aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga acagtttatt 1920
cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag aaaaaaaaag 1980
aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt ccattctctt 2040
agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac ctcaatggag 2100
tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc atgtatctat 2160
ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa agctgaaaaa 2220
aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt atataaagac 2280
ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat tctactttta 2340
tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata aacacacata 2400
aacaaacaaa atgtccgctg ttactccaat gagtagagtt gtccctaatc aagcattgat 2460
tggtttgact ttagcaggtt tgattgctgc tgcttggttg acattgcata tatacggtgt 2520
ctacttccac agatggacaa tctggtctgt tttgaccgtc ccattaattg tagctggtca 2580
aacttggttg tccgtaggtt tattcatagt tgctcatgat gcaatgcacg gtagtttggc 2640
accagccaga cctagattaa acacagcaat cggttctttg gctttagcat tgtatgccgg 2700
ttttagattc accccattga aaactgccca tcacgctcat cacgctgcac caggtacagc 2760
agatgaccct gattttcatg ccgacgctcc aagagcattt ttgccttggt tctatggttt 2820
ctttagaacc tacttcggtt ggagagaatt agcagttttg actgtattag ttgcagtcgc 2880
cgtattaata ttgggtgcta gaatgccaaa tttgttagtc ttttgggccg ctcctgcatt 2940
gttgtctgcc ttacaattgt tcactttcgg tacatggttg ccacatagac acaccgatga 3000
cgcctttcct gataatcata acgctagaac atctccattc ggtcctgttt tatcattgtt 3060
gacctgtttc catttcggta gacatcacga acatcacttg acaccttgga agccttggtg 3120
gtctttattt tcataaattt aactccttaa gttactttaa tgatttagtt tttattatta 3180
ataattcatg ctcatgacat ctcatataca cgtttataaa acttaaatag attgaaaatg 3240
tattaaagat tcctcaggga ttcgattttt ttggaagttt ttgttttttt ttccttgaga 3300
tgctgtagta tttgggaaca attatacaat cgaaagatat atgcttacat tcgaccgttt 3360
tagccgtgat cattatccta tagtaacata acctgaagca taactgacac tactatcatc 3420
aatacttgtc acatgagaac tctgtgaata attaggccac tgaaatttga tgcctgaagg 3480
accggcatca cggattttcg ataaagcact tagtatcaca ctaattggct tttcgctcca 3540
actggcaccg ctggcttgaa caacaatacc agccttccaa cttctgtaaa taacggcggt 3600
acgccagtgc caccagtacc gttacctttc ggtatacctc ctttccccat gtttccaatg 3660
cccttcatgc ctccaacggc tactatcaca aatcctcatc aagctgacgc aagccctaag 3720
aaatgaataa caatactgac agtactaaat aattgcctac ttggcttcac atacgttgca 3780
tacgtcgata tagataataa tgataatgac agcaggatta tcgtaatacg taatagttga 3840
aaatctcaaa aatgtgtggg tcattacgta aataatgata ggaatgggat tcttctattt 3900
ttcctttttc cattctagca gccgtcggga aaacgtggca tcctctcttt cgggctcaat 3960
tggagtcacg ctgccgtgag catcctctct ttccatatct aacaactgag cacgtaacca 4020
atggaaaagc atgagcttag cgttgctcca aaaaagtatt ggatggttaa taccatttgt 4080
ctgttctctt ctgactttga ctcctcaaaa aaaaaaaatc tacaatcaac agatcgcttc 4140
aattacgccc tcacaaaaac ttttttcctt cttcttcgcc cacgttaaat tttatccctc 4200
atgttgtcta acggatttct gcacttgatt tattataaaa agacaaagac ataatacttc 4260
tctatcaatt tcagttattg ttcttccttg cgttattctt ctgttcttct ttttcttttg 4320
tcatatataa ccataaccaa gtaatacata ttcaaaatgt ccgctgttac tccaatgagt 4380
agagttgtcc ctaatcaagc attgattggt ttgactttag caggtttgat tgctgctgct 4440
tggttgacat tgcatatata cggtgtctac ttccacagat ggacaatctg gtctgttttg 4500
accgtcccat taattgtagc tggtcaaact tggttgtccg taggtttatt catagttgct 4560
catgatgcaa tgcacggtag tttggcacca gccagaccta gattaaacac agcaatcggt 4620
tctttggctt tagcattgta tgccggtttt agattcaccc cattgaaaac tgcccatcac 4680
gctcatcacg ctgcaccagg tacagcagat gaccctgatt ttcatgccga cgctccaaga 4740
gcatttttgc cttggttcta tggtttcttt agaacctact tcggttggag agaattagca 4800
gttttgactg tattagttgc agtcgccgta ttaatattgg gtgctagaat gccaaatttg 4860
ttagtctttt gggccgctcc tgcattgttg tctgccttac aattgttcac tttcggtaca 4920
tggttgccac atagacacac cgatgacgcc tttcctgata atcataacgc tagaacatct 4980
ccattcggtc ctgttttatc attgttgacc tgtttccatt tcggtagaca tcacgaacat 5040
cacttgacac cttggaagcc ttggtggtct ttattttcat aaagagtaat aattattgct 5100
tccatataat atttttatat acctcttatt tttatgtatt agttaattaa gtatttttat 5160
ctatctgctt atcattttct tttcatatag ggggggttgg tgttttcttg cccatcagat 5220
tgatgtcctc caactcggca ctattttaca aagggttttt ttgtaagaga aggagaagac 5280
agatactaaa ccatacgtta ctcgaaacaa aaaaaaaaaa aatggaaaaa gctgctatca 5340
acaaaagacg gcctcatcaa acctaaagaa accatgtcag cgttatagtt ttttctcctt 5400
gacgttaaag tatagaggta tattaacaat tttttgttga tacttttatg acatttgaat 5460
aagaagtaat acaaaccgaa aatgttgaaa gtattagtta aagtggttat gcagcttttg 5520
catttatata tctgttaata gatcaaaaat catcgcttcg ctgattaatt accccagaaa 5580
taaggctaaa aaactaatcg cattattatc ctatggttgt taatttgatt cgttgatttg 5640
aaggtttgtg gggccaggtt actgccaatt tttcctcttc ataaccataa aagctagtat 5700
tgtagaatct ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt tcaggaacgc 5760
gaccggtgaa gaccaggacg cacggaggag agtcttccgt cggagggctg tcgcccgctc 5820
ggcggcttct aatccgtact tcaatatagc aatgagcagt taagcgtatt actgaaagtt 5880
ccaaagagaa ggttttttta ggctaagata atggggctct ttacatttcc acaacatata 5940
agtaagatta gatatggata tgtatatggt ggtattgcca tgtaatatga ttattaaact 6000
tctttgcgtc catccaaaaa aaaagtaaga atttttgaaa attcaatata aatgactaac 6060
ttcttgatcg ttgttgctac tgttttggtt atggaattga ctgcttactc tgttcacaga 6120
tggatcatgc acggtccatt gggttggggt tggcacaagt ctcaccacga agaacacgac 6180
cacgctttgg aaaagaacga cttgtacggt ttggttttcg ctgttatcgc tactgttttg 6240
ttcactgttg gttggatctg ggctccagtt ttgtggtgga tcgctttggg tatgactgtt 6300
tacggtttga tctacttcgt tttgcacgac ggtttggttc accaaagatg gccattcaga 6360
tacatcccaa gaaagggtta cgctagaaga ttgtaccaag ctcacagatt gcaccacgct 6420
gttgaaggta gagaccactg tgtttctttc ggtttcatct acgctccacc agttgacaag 6480
ttgaagcaag acttgaagat gtctggtgtt ttgagagctg aagctcaaga aagaacttaa 6540
agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg tcggcttgtc 6600
taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta gatacgttgt 6660
tgacacttct aaataagcga atttcttatg atttatgatt tttattatta aataagttat 6720
aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt 6780
attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc 6840
tcttattgac cacacctcta ccggcatggt cgac 6874
<210> 67
<211> 12549
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 67
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 240
ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 300
agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 360
cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 420
cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 480
ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 540
aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 600
tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 660
ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 720
aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 780
acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 840
aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 900
gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 960
ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 1020
ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 1080
atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 1140
gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 1200
gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 1260
aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 1320
agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 1380
tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 1440
tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 1500
agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 1560
gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 1620
aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 1680
cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 1740
gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 1800
gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 1860
cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 1920
taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat 1980
acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac catgccggta 2040
gaggtgtggt caataagagc gacctcatgc tatacctgag aaagcaacct gacctacagg 2100
aaagagttac tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg 2160
tatacactta ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc 2220
gcttatttag aagtgtcaac aacgtatcta ccaacgattt gacccttttc catcttttcg 2280
taaatttctg gcaaggtaga caagccgaca accttgattg gagacttgac caaacctctg 2340
gcgaagaagt ccaaagcttt aagttctttc ttgagcttca gctctcaaaa caccagacat 2400
cttcaagtct tgcttcaact tgtcaactgg tggagcgtag atgaaaccga aagaaacaca 2460
gtggtctcta ccttcaacag cgtggtgcaa tctgtgagct tggtacaatc ttctagcgta 2520
accctttctt gggatgtatc tgaatggcca tctttggtga accaaaccgt cgtgcaaaac 2580
gaagtagatc aaaccgtaaa cagtcatacc caaagcgatc caccacaaaa ctggagccca 2640
gatccaacca acagtgaaca aaacagtagc gataacagcg aaaaccaaac cgtacaagtc 2700
gttcttttcc aaagcgtggt cgtgttcttc gtggtgagac ttgtgccaac cccaacccaa 2760
tggaccgtgc atgatccatc tgtgaacaga gtaagcagtc aattccataa ccaaaacagt 2820
agcaacaacg atcaagaagt tagtcattta tattgaattt tcaaaaattc ttactttttt 2880
tttggatgga cgcaaagaag tttaataatc atattacatg gcaataccac catatacata 2940
tccatatcta atcttactta tatgttgtgg aaatgtaaag agccccatta tcttagccta 3000
aaaaaacctt ctctttggaa ctttcagtaa tacgcttaac tgctcattgc tatattgaag 3060
tacggattag aagccgccga gcgggcgaca gccctccgac ggaagactct cctccgtgcg 3120
tcctggtctt caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg 3180
aacaataaag attctacaat actagctttt atggttatga agaggaaaaa ttggcagtaa 3240
cctggcccca caaaccttca aatcaacgaa tcaaattaac aaccatagga taataatgcg 3300
attagttttt tagccttatt tctggggtaa ttaatcagcg aagcgatgat ttttgatcta 3360
ttaacagata tataaatgca aaagctgcat aaccacttta actaatactt tcaacatttt 3420
cggtttgtat tacttcttat tcaaatgtca taaaagtatc aacaaaaaat tgttaatata 3480
cctctatact ttaacgtcaa ggagaaaaaa ctataacgct gacatggttt ctttaggttt 3540
gatgaggccg tcttttgttg atagcagctt tttccatttt tttttttttt gtttcgagta 3600
acgtatggtt tagtatctgt cttctccttc tcttacaaaa aaaccctttg taaaatagtg 3660
ccgagttgga ggacatcaat ctgatgggca agaaaacacc aaccccccct atatgaaaag 3720
aaaatgataa gcagatagat aaaaatactt aattaactaa tacataaaaa taagaggtat 3780
ataaaaatat tatatggaag caataattat tactctttat gaaaataaag accaccaagg 3840
cttccaaggt gtcaagtgat gttcgtgatg tctaccgaaa tggaaacagg tcaacaatga 3900
taaaacagga ccgaatggag atgttctagc gttatgatta tcaggaaagg cgtcatcggt 3960
gtgtctatgt ggcaaccatg taccgaaagt gaacaattgt aaggcagaca acaatgcagg 4020
agcggcccaa aagactaaca aatttggcat tctagcaccc aatattaata cggcgactgc 4080
aactaataca gtcaaaactg ctaattctct ccaaccgaag taggttctaa agaaaccata 4140
gaaccaaggc aaaaatgctc ttggagcgtc ggcatgaaaa tcagggtcat ctgctgtacc 4200
tggtgcagcg tgatgagcgt gatgggcagt tttcaatggg gtgaatctaa aaccggcata 4260
caatgctaaa gccaaagaac cgattgctgt gtttaatcta ggtctggctg gtgccaaact 4320
accgtgcatt gcatcatgag caactatgaa taaacctacg gacaaccaag tttgaccagc 4380
tacaattaat gggacggtca aaacagacca gattgtccat ctgtggaagt agacaccgta 4440
tatatgcaat gtcaaccaag cagcagcaat caaacctgct aaagtcaaac caatcaatgc 4500
ttgattaggg acaactctac tcattggagt aacagcggac attttgaata tgtattactt 4560
ggttatggtt atatatgaca aaagaaaaag aagaacagaa gaataacgca aggaagaaca 4620
ataactgaaa ttgatagaga agtattatgt ctttgtcttt ttataataaa tcaagtgcag 4680
aaatccgtta gacaacatga gggataaaat ttaacgtggg cgaagaagaa ggaaaaaagt 4740
ttttgtgagg gcgtaattga agcgatctgt tgattgtaga tttttttttt ttgaggagtc 4800
aaagtcagaa gagaacagac aaatggtatt aaccatccaa tacttttttg gagcaacgct 4860
aagctcatgc ttttccattg gttacgtgct cagttgttag atatggaaag agaggatgct 4920
cacggcagcg tgactccaat tgagcccgaa agagaggatg ccacgttttc ccgacggctg 4980
ctagaatgga aaaaggaaaa atagaagaat cccattccta tcattattta cgtaatgacc 5040
cacacatttt tgagattttc aactattacg tattacgata atcctgctgt cattatcatt 5100
attatctata tcgacgtatg caacgtatgt gaagccaagt aggcaattat ttagtactgt 5160
cagtattgtt attcatttct tagggcttgc gtcagcttga tgaggatttg tgatagtagc 5220
cgttggaggc atgaagggca ttggaaacat ggggaaagga ggtataccga aaggtaacgg 5280
tactggtggc actggcgtac cgccgttatt tacagaagtt ggaaggctgg tattgttgtt 5340
caagccagcg gtgccagttg gagcgaaaag ccaattagtg tgatactaag tgctttatcg 5400
aaaatccgtg atgccggtcc ttcaggcatc aaatttcagt ggcctaatta ttcacagagt 5460
tctcatgtga caagtattga tgatagtagt gtcagttatg cttcaggtta tgttactata 5520
ggataatgat cacggctaaa acggtcgaat gtaagcatat atctttcgat tgtataattg 5580
ttcccaaata ctacagcatc tcaaggaaaa aaaaacaaaa acttccaaaa aaatcgaatc 5640
cctgaggaat ctttaataca ttttcaatct atttaagttt tataaacgtg tatatgagat 5700
gtcatgagca tgaattatta ataataaaaa ctaaatcatt aaagtaactt aaggagttaa 5760
atttatgaaa ataaagacca ccaaggcttc caaggtgtca agtgatgttc gtgatgtcta 5820
ccgaaatgga aacaggtcaa caatgataaa acaggaccga atggagatgt tctagcgtta 5880
tgattatcag gaaaggcgtc atcggtgtgt ctatgtggca accatgtacc gaaagtgaac 5940
aattgtaagg cagacaacaa tgcaggagcg gcccaaaaga ctaacaaatt tggcattcta 6000
gcacccaata ttaatacggc gactgcaact aatacagtca aaactgctaa ttctctccaa 6060
ccgaagtagg ttctaaagaa accatagaac caaggcaaaa atgctcttgg agcgtcggca 6120
tgaaaatcag ggtcatctgc tgtacctggt gcagcgtgat gagcgtgatg ggcagttttc 6180
aatggggtga atctaaaacc ggcatacaat gctaaagcca aagaaccgat tgctgtgttt 6240
aatctaggtc tggctggtgc caaactaccg tgcattgcat catgagcaac tatgaataaa 6300
cctacggaca accaagtttg accagctaca attaatggga cggtcaaaac agaccagatt 6360
gtccatctgt ggaagtagac accgtatata tgcaatgtca accaagcagc agcaatcaaa 6420
cctgctaaag tcaaaccaat caatgcttga ttagggacaa ctctactcat tggagtaaca 6480
gcggacattt tgtttgttta tgtgtgttta ttcgaaacta agttcttggt gttttaaaac 6540
taaaaaaaag actaactata aaagtagaat ttaagaagtt taagaaatag atttacagaa 6600
ttacaatcaa tacctaccgt ctttatatac ttattagtca agtaggggaa taatttcagg 6660
gaactggttt caaccttttt tttcagcttt ttccaaatca gagagagcag aaggtaatag 6720
aaggtgtaag aaaatgagat agatacatgc gtgggtcaat tgccttgtgt catcatttac 6780
tccaggcagg ttgcatcact ccattgaggt tgtgcccgtt ttttgcctgt ttgtgcccct 6840
gttctctgta gttgcgctaa gagaatggac ctatgaactg atggttggtg aagaaaacaa 6900
tattttggtg ctgggattct ttttttttct ggatgccagc ttaaaaagcg ggctccatta 6960
tatttagtgg atgccaggaa taaactgttc acccagacac ctacgatgtt atatattctg 7020
tgtaacccgc cccctatttt gggcatgtac gggttacagc agaattaaaa ggctaatttt 7080
ttgactaaat aaagttagga aaatcactac tattaattat ttacgtattc tttgaaatgg 7140
cagtattgat aatgataaac tcgaactgag agtgtaaact gcgaagcttg caaattaaag 7200
ccttcgagcg tcccaaaacc ttctcaagca aggttttcag tataatgtta catgcgtaca 7260
cgcgtttgta cagaaaaaaa agaaaaattt gaaatataaa taacgttctt aatactaaca 7320
taactattaa aaaaaataaa tagggaccta gacttcaggt tgtctaactc cttccttttc 7380
ggttagagcg gatgtgggag gagggcgtga atgtaagcgt gacataacta attacatgat 7440
atcgacaaag gaaaaggggc ctgttttatg aaaataaaga ccaccaaggc ttccaaggtg 7500
tcaagtgatg ttcgtgatgt ctaccgaaat ggaaacaggt caacaatgat aaaacaggac 7560
cgaatggaga tgttctagcg ttatgattat caggaaaggc gtcatcggtg tgtctatgtg 7620
gcaaccatgt accgaaagtg aacaattgta aggcagacaa caatgcagga gcggcccaaa 7680
agactaacaa atttggcatt ctagcaccca atattaatac ggcgactgca actaatacag 7740
tcaaaactgc taattctctc caaccgaagt aggttctaaa gaaaccatag aaccaaggca 7800
aaaatgctct tggagcgtcg gcatgaaaat cagggtcatc tgctgtacct ggtgcagcgt 7860
gatgagcgtg atgggcagtt ttcaatgggg tgaatctaaa accggcatac aatgctaaag 7920
ccaaagaacc gattgctgtg tttaatctag gtctggctgg tgccaaacta ccgtgcattg 7980
catcatgagc aactatgaat aaacctacgg acaaccaagt ttgaccagct acaattaatg 8040
ggacggtcaa aacagaccag attgtccatc tgtggaagta gacaccgtat atatgcaatg 8100
tcaaccaagc agcagcaatc aaacctgcta aagtcaaacc aatcaatgct tgattaggga 8160
caactctact cattggagta acagcggaca tttttttgat taaaattaaa aaaacttttt 8220
gtttttgtgt ttattctttg ttcttagaaa agacaagttg agcttgtttg ttcttgatgt 8280
tttattattt tacaatagct gcaaatgaag aatagattcg aacattgtga agtattggca 8340
tatatcgtct ctatttatac tttttttttt tcagttctag tatattttgt attttcctcc 8400
ttttcattct ttcagttgcc aataagttac aggggatctc gaaagatggt ggggattttt 8460
ccttgaaaga cgactttttg ccatctaatt tttccttgtt gcctctgaaa attatccagc 8520
agaagcaaat gtaaaagatg aacctcagaa gaacacgcag gggcccgaaa ttgttcctac 8580
gagaagtagt gggtcataaa aagtttattc cctggaaaaa aaattttgcg ttgcctttct 8640
ggagaatttt ttcgaattag cgtgctgcca ctgcatgcat ttctgagaag tgtgggcatt 8700
cttccaccag ttgttcctcc taaaaaaaaa aaagatttcc taccccgcac aaattcctgc 8760
atacccctca tttccacggg gtaaaaaaaa aacatcccgt agcagcctct gacggagccg 8820
tatggaacaa tcctggataa atatggcgga aaaacggctc tggctgcggc tttgtttaag 8880
gcagaaacta gaggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt 8940
ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc 9000
tgtgtgaaat tgttatccgc tcacaattcc acacaacata ggagccggaa gcataaagtg 9060
taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc gctcactgcc 9120
cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 9180
gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 9240
ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 9300
agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 9360
ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 9420
caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 9480
gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 9540
cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 9600
tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 9660
gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 9720
cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 9780
tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 9840
tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 9900
caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 9960
aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 10020
cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 10080
ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 10140
tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 10200
atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 10260
tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 10320
aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 10380
catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 10440
gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 10500
ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 10560
aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 10620
atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 10680
cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 10740
gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 10800
agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 10860
gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 10920
caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 10980
ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 11040
tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 11100
aggggttccg cgcacatttc cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt 11160
ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca 11220
tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt 11280
catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag 11340
ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta 11400
tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc 11460
ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc 11520
actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa 11580
aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt 11640
tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga 11700
acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct 11760
attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca 11820
ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat 11880
aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt 11940
tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg 12000
aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga 12060
aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta 12120
gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa 12180
atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt 12240
gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta 12300
cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc 12360
cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct 12420
gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt gatattggat 12480
catactaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 12540
cctttcgtc 12549
<210> 68
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 68
ggatcctcta gtttctgcct taaacaaagc c 31
<210> 69
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 69
tgaaaattca atataacatg ccggtagagg tgtggtc 37
<210> 70
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 70
cctctaccgg catgttatat tgaattttca aaaattctta c 41
<210> 71
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 71
gtcgacttaa atgcttttct tctttttatt agaaaaagcg c 41
<210> 72
<211> 8246
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 72
ggatcctcta gtttctgcct taaacaaagc cgcagccaga gccgtttttc cgccatattt 60
atccaggatt gttccatacg gctccgtcag aggctgctac gggatgtttt ttttttaccc 120
cgtggaaatg aggggtatgc aggaatttgt gcggggtagg aaatcttttt tttttttagg 180
aggaacaact ggtggaagaa tgcccacact tctcagaaat gcatgcagtg gcagcacgct 240
aattcgaaaa aattctccag aaaggcaacg caaaattttt tttccaggga ataaactttt 300
tatgacccac tacttctcgt aggaacaatt tcgggcccct gcgtgttctt ctgaggttca 360
tcttttacat ttgcttctgc tggataattt tcagaggcaa caaggaaaaa ttagatggca 420
aaaagtcgtc tttcaaggaa aaatccccac catctttcga gatcccctgt aacttattgg 480
caactgaaag aatgaaaagg aggaaaatac aaaatatact agaactgaaa aaaaaaaagt 540
ataaatagag acgatatatg ccaatacttc acaatgttcg aatctattct tcatttgcag 600
ctattgtaaa ataataaaac atcaagaaca aacaagctca acttgtcttt tctaagaaca 660
aagaataaac acaaaaacaa aaagtttttt taattttaat caaaaaaatg tccgctgtta 720
ctccaatgag tagagttgtc cctaatcaag cattgattgg tttgacttta gcaggtttga 780
ttgctgctgc ttggttgaca ttgcatatat acggtgtcta cttccacaga tggacaatct 840
ggtctgtttt gaccgtccca ttaattgtag ctggtcaaac ttggttgtcc gtaggtttat 900
tcatagttgc tcatgatgca atgcacggta gtttggcacc agccagacct agattaaaca 960
cagcaatcgg ttctttggct ttagcattgt atgccggttt tagattcacc ccattgaaaa 1020
ctgcccatca cgctcatcac gctgcaccag gtacagcaga tgaccctgat tttcatgccg 1080
acgctccaag agcatttttg ccttggttct atggtttctt tagaacctac ttcggttgga 1140
gagaattagc agttttgact gtattagttg cagtcgccgt attaatattg ggtgctagaa 1200
tgccaaattt gttagtcttt tgggccgctc ctgcattgtt gtctgcctta caattgttca 1260
ctttcggtac atggttgcca catagacaca ccgatgacgc ctttcctgat aatcataacg 1320
ctagaacatc tccattcggt cctgttttat cattgttgac ctgtttccat ttcggtagac 1380
atcacgaaca tcacttgaca ccttggaagc cttggtggtc tttattttca taaaacaggc 1440
cccttttcct ttgtcgatat catgtaatta gttatgtcac gcttacattc acgccctcct 1500
cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt 1560
tatttttttt aatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt 1620
tttttctgta caaacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg 1680
ttttgggacg ctcgaaggct ttaatttgca agcttcgcag tttacactct cagttcgagt 1740
ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt agtgattttc 1800
ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt acatgcccaa 1860
aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga acagtttatt 1920
cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag aaaaaaaaag 1980
aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt ccattctctt 2040
agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac ctcaatggag 2100
tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc atgtatctat 2160
ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa agctgaaaaa 2220
aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt atataaagac 2280
ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat tctactttta 2340
tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata aacacacata 2400
aacaaacaaa atgtccgctg ttactccaat gagtagagtt gtccctaatc aagcattgat 2460
tggtttgact ttagcaggtt tgattgctgc tgcttggttg acattgcata tatacggtgt 2520
ctacttccac agatggacaa tctggtctgt tttgaccgtc ccattaattg tagctggtca 2580
aacttggttg tccgtaggtt tattcatagt tgctcatgat gcaatgcacg gtagtttggc 2640
accagccaga cctagattaa acacagcaat cggttctttg gctttagcat tgtatgccgg 2700
ttttagattc accccattga aaactgccca tcacgctcat cacgctgcac caggtacagc 2760
agatgaccct gattttcatg ccgacgctcc aagagcattt ttgccttggt tctatggttt 2820
ctttagaacc tacttcggtt ggagagaatt agcagttttg actgtattag ttgcagtcgc 2880
cgtattaata ttgggtgcta gaatgccaaa tttgttagtc ttttgggccg ctcctgcatt 2940
gttgtctgcc ttacaattgt tcactttcgg tacatggttg ccacatagac acaccgatga 3000
cgcctttcct gataatcata acgctagaac atctccattc ggtcctgttt tatcattgtt 3060
gacctgtttc catttcggta gacatcacga acatcacttg acaccttgga agccttggtg 3120
gtctttattt tcataaattt aactccttaa gttactttaa tgatttagtt tttattatta 3180
ataattcatg ctcatgacat ctcatataca cgtttataaa acttaaatag attgaaaatg 3240
tattaaagat tcctcaggga ttcgattttt ttggaagttt ttgttttttt ttccttgaga 3300
tgctgtagta tttgggaaca attatacaat cgaaagatat atgcttacat tcgaccgttt 3360
tagccgtgat cattatccta tagtaacata acctgaagca taactgacac tactatcatc 3420
aatacttgtc acatgagaac tctgtgaata attaggccac tgaaatttga tgcctgaagg 3480
accggcatca cggattttcg ataaagcact tagtatcaca ctaattggct tttcgctcca 3540
actggcaccg ctggcttgaa caacaatacc agccttccaa cttctgtaaa taacggcggt 3600
acgccagtgc caccagtacc gttacctttc ggtatacctc ctttccccat gtttccaatg 3660
cccttcatgc ctccaacggc tactatcaca aatcctcatc aagctgacgc aagccctaag 3720
aaatgaataa caatactgac agtactaaat aattgcctac ttggcttcac atacgttgca 3780
tacgtcgata tagataataa tgataatgac agcaggatta tcgtaatacg taatagttga 3840
aaatctcaaa aatgtgtggg tcattacgta aataatgata ggaatgggat tcttctattt 3900
ttcctttttc cattctagca gccgtcggga aaacgtggca tcctctcttt cgggctcaat 3960
tggagtcacg ctgccgtgag catcctctct ttccatatct aacaactgag cacgtaacca 4020
atggaaaagc atgagcttag cgttgctcca aaaaagtatt ggatggttaa taccatttgt 4080
ctgttctctt ctgactttga ctcctcaaaa aaaaaaaatc tacaatcaac agatcgcttc 4140
aattacgccc tcacaaaaac ttttttcctt cttcttcgcc cacgttaaat tttatccctc 4200
atgttgtcta acggatttct gcacttgatt tattataaaa agacaaagac ataatacttc 4260
tctatcaatt tcagttattg ttcttccttg cgttattctt ctgttcttct ttttcttttg 4320
tcatatataa ccataaccaa gtaatacata ttcaaaatgt ccgctgttac tccaatgagt 4380
agagttgtcc ctaatcaagc attgattggt ttgactttag caggtttgat tgctgctgct 4440
tggttgacat tgcatatata cggtgtctac ttccacagat ggacaatctg gtctgttttg 4500
accgtcccat taattgtagc tggtcaaact tggttgtccg taggtttatt catagttgct 4560
catgatgcaa tgcacggtag tttggcacca gccagaccta gattaaacac agcaatcggt 4620
tctttggctt tagcattgta tgccggtttt agattcaccc cattgaaaac tgcccatcac 4680
gctcatcacg ctgcaccagg tacagcagat gaccctgatt ttcatgccga cgctccaaga 4740
gcatttttgc cttggttcta tggtttcttt agaacctact tcggttggag agaattagca 4800
gttttgactg tattagttgc agtcgccgta ttaatattgg gtgctagaat gccaaatttg 4860
ttagtctttt gggccgctcc tgcattgttg tctgccttac aattgttcac tttcggtaca 4920
tggttgccac atagacacac cgatgacgcc tttcctgata atcataacgc tagaacatct 4980
ccattcggtc ctgttttatc attgttgacc tgtttccatt tcggtagaca tcacgaacat 5040
cacttgacac cttggaagcc ttggtggtct ttattttcat aaagagtaat aattattgct 5100
tccatataat atttttatat acctcttatt tttatgtatt agttaattaa gtatttttat 5160
ctatctgctt atcattttct tttcatatag ggggggttgg tgttttcttg cccatcagat 5220
tgatgtcctc caactcggca ctattttaca aagggttttt ttgtaagaga aggagaagac 5280
agatactaaa ccatacgtta ctcgaaacaa aaaaaaaaaa aatggaaaaa gctgctatca 5340
acaaaagacg gcctcatcaa acctaaagaa accatgtcag cgttatagtt ttttctcctt 5400
gacgttaaag tatagaggta tattaacaat tttttgttga tacttttatg acatttgaat 5460
aagaagtaat acaaaccgaa aatgttgaaa gtattagtta aagtggttat gcagcttttg 5520
catttatata tctgttaata gatcaaaaat catcgcttcg ctgattaatt accccagaaa 5580
taaggctaaa aaactaatcg cattattatc ctatggttgt taatttgatt cgttgatttg 5640
aaggtttgtg gggccaggtt actgccaatt tttcctcttc ataaccataa aagctagtat 5700
tgtagaatct ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt tcaggaacgc 5760
gaccggtgaa gaccaggacg cacggaggag agtcttccgt cggagggctg tcgcccgctc 5820
ggcggcttct aatccgtact tcaatatagc aatgagcagt taagcgtatt actgaaagtt 5880
ccaaagagaa ggttttttta ggctaagata atggggctct ttacatttcc acaacatata 5940
agtaagatta gatatggata tgtatatggt ggtattgcca tgtaatatga ttattaaact 6000
tctttgcgtc catccaaaaa aaaagtaaga atttttgaaa attcaatata aatgactaac 6060
ttcttgatcg ttgttgctac tgttttggtt atggaattga ctgcttactc tgttcacaga 6120
tggatcatgc acggtccatt gggttggggt tggcacaagt ctcaccacga agaacacgac 6180
cacgctttgg aaaagaacga cttgtacggt ttggttttcg ctgttatcgc tactgttttg 6240
ttcactgttg gttggatctg ggctccagtt ttgtggtgga tcgctttggg tatgactgtt 6300
tacggtttga tctacttcgt tttgcacgac ggtttggttc accaaagatg gccattcaga 6360
tacatcccaa gaaagggtta cgctagaaga ttgtaccaag ctcacagatt gcaccacgct 6420
gttgaaggta gagaccactg tgtttctttc ggtttcatct acgctccacc agttgacaag 6480
ttgaagcaag acttgaagat gtctggtgtt ttgagagctg aagctcaaga aagaacttaa 6540
agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg tcggcttgtc 6600
taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta gatacgttgt 6660
tgacacttct aaataagcga atttcttatg atttatgatt tttattatta aataagttat 6720
aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt 6780
attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc 6840
tcttattgac cacacctcta ccggcatgtt atattgaatt ttcaaaaatt cttacttttt 6900
ttttggatgg acgcaaagaa gtttaataat catattacat ggcaatacca ccatatacat 6960
atccatatct aatcttactt atatgttgtg gaaatgtaaa gagccccatt atcttagcct 7020
aaaaaaacct tctctttgga actttcagta atacgcttaa ctgctcattg ctatattgaa 7080
gtacggatta gaagccgccg agcgggcgac agccctccga cggaagactc tcctccgtgc 7140
gtcctggtct tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc gcactgctcc 7200
gaacaataaa gattctacaa tactagcttt tatggttatg aagaggaaaa attggcagta 7260
acctggcccc acaaaccttc aaatcaacga atcaaattaa caaccatagg ataataatgc 7320
gattagtttt ttagccttat ttctggggta attaatcagc gaagcgatga tttttgatct 7380
attaacagat atataaatgc aaaagctgca taaccacttt aactaatact ttcaacattt 7440
tcggtttgta ttacttctta ttcaaatgtc ataaaagtat caacaaaaaa ttgttaatat 7500
acctctatac tttaacgtca aggagaaaaa actataatga ctaacttctt gatcgttgtt 7560
gctactgttt tggttatgga attgactgct tactctgttc acagatggat catgcacggt 7620
ccattgggtt ggggttggca caagtctcac cacgaagaac acgaccacgc tttggaaaag 7680
aacgacttgt acggtttggt tttcgctgtt atcgctactg ttttgttcac tgttggttgg 7740
atctgggctc cagttttgtg gtggatcgct ttgggtatga ctgtttacgg tttgatctac 7800
ttcgttttgc acgacggttt ggttcaccaa agatggccat tcagatacat cccaagaaag 7860
ggttacgcta gaagattgta ccaagctcac agattgcacc acgctgttga aggtagagac 7920
cactgtgttt ctttcggttt catctacgct ccaccagttg acaagttgaa gcaagacttg 7980
aagatgtctg gtgttttgag agctgaagct caagaaagaa cttaaagatt aatataatta 8040
tataaaaata ttatcttctt ttctttatat ctagtgttat gtaaaataaa ttgatgacta 8100
cggaaagctt ttttatattg tttctttttc attctgagcc acttaaattt cgtgaatgtt 8160
cttgtaaggg acggtagatt tacaagtgat acaacaaaaa gcaaggcgct ttttctaata 8220
aaaagaagaa aagcatttaa gtcgac 8246
<210> 73
<211> 13921
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 73
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 240
ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 300
agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 360
cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 420
cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 480
ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 540
aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 600
tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 660
ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 720
aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 780
acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 840
aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 900
gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 960
ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 1020
ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 1080
atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 1140
gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 1200
gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 1260
aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 1320
agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 1380
tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 1440
tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 1500
agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 1560
gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 1620
aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 1680
cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 1740
gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 1800
gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 1860
cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 1920
taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat 1980
acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ttaaatgctt 2040
ttcttctttt tattagaaaa agcgccttgc tttttgttgt atcacttgta aatctaccgt 2100
cccttacaag aacattcacg aaatttaagt ggctcagaat gaaaaagaaa caatataaaa 2160
aagctttccg tagtcatcaa tttattttac ataacactag atataaagaa aagaagataa 2220
tatttttata taattatatt aatctttaag ttctttcttg agcttcagct ctcaaaacac 2280
cagacatctt caagtcttgc ttcaacttgt caactggtgg agcgtagatg aaaccgaaag 2340
aaacacagtg gtctctacct tcaacagcgt ggtgcaatct gtgagcttgg tacaatcttc 2400
tagcgtaacc ctttcttggg atgtatctga atggccatct ttggtgaacc aaaccgtcgt 2460
gcaaaacgaa gtagatcaaa ccgtaaacag tcatacccaa agcgatccac cacaaaactg 2520
gagcccagat ccaaccaaca gtgaacaaaa cagtagcgat aacagcgaaa accaaaccgt 2580
acaagtcgtt cttttccaaa gcgtggtcgt gttcttcgtg gtgagacttg tgccaacccc 2640
aacccaatgg accgtgcatg atccatctgt gaacagagta agcagtcaat tccataacca 2700
aaacagtagc aacaacgatc aagaagttag tcattatagt tttttctcct tgacgttaaa 2760
gtatagaggt atattaacaa ttttttgttg atacttttat gacatttgaa taagaagtaa 2820
tacaaaccga aaatgttgaa agtattagtt aaagtggtta tgcagctttt gcatttatat 2880
atctgttaat agatcaaaaa tcatcgcttc gctgattaat taccccagaa ataaggctaa 2940
aaaactaatc gcattattat cctatggttg ttaatttgat tcgttgattt gaaggtttgt 3000
ggggccaggt tactgccaat ttttcctctt cataaccata aaagctagta ttgtagaatc 3060
tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga 3120
agaccaggac gcacggagga gagtcttccg tcggagggct gtcgcccgct cggcggcttc 3180
taatccgtac ttcaatatag caatgagcag ttaagcgtat tactgaaagt tccaaagaga 3240
aggttttttt aggctaagat aatggggctc tttacatttc cacaacatat aagtaagatt 3300
agatatggat atgtatatgg tggtattgcc atgtaatatg attattaaac ttctttgcgt 3360
ccatccaaaa aaaaagtaag aatttttgaa aattcaatat aacatgccgg tagaggtgtg 3420
gtcaataaga gcgacctcat gctatacctg agaaagcaac ctgacctaca ggaaagagtt 3480
actcaagaat aagaattttc gttttaaaac ctaagagtca ctttaaaatt tgtatacact 3540
tatttttttt ataacttatt taataataaa aatcataaat cataagaaat tcgcttattt 3600
agaagtgtca acaacgtatc taccaacgat ttgacccttt tccatctttt cgtaaatttc 3660
tggcaaggta gacaagccga caaccttgat tggagacttg accaaacctc tggcgaagaa 3720
gtccaaagct ttaagttctt tcttgagctt cagctctcaa aacaccagac atcttcaagt 3780
cttgcttcaa cttgtcaact ggtggagcgt agatgaaacc gaaagaaaca cagtggtctc 3840
taccttcaac agcgtggtgc aatctgtgag cttggtacaa tcttctagcg taaccctttc 3900
ttgggatgta tctgaatggc catctttggt gaaccaaacc gtcgtgcaaa acgaagtaga 3960
tcaaaccgta aacagtcata cccaaagcga tccaccacaa aactggagcc cagatccaac 4020
caacagtgaa caaaacagta gcgataacag cgaaaaccaa accgtacaag tcgttctttt 4080
ccaaagcgtg gtcgtgttct tcgtggtgag acttgtgcca accccaaccc aatggaccgt 4140
gcatgatcca tctgtgaaca gagtaagcag tcaattccat aaccaaaaca gtagcaacaa 4200
cgatcaagaa gttagtcatt tatattgaat tttcaaaaat tcttactttt tttttggatg 4260
gacgcaaaga agtttaataa tcatattaca tggcaatacc accatataca tatccatatc 4320
taatcttact tatatgttgt ggaaatgtaa agagccccat tatcttagcc taaaaaaacc 4380
ttctctttgg aactttcagt aatacgctta actgctcatt gctatattga agtacggatt 4440
agaagccgcc gagcgggcga cagccctccg acggaagact ctcctccgtg cgtcctggtc 4500
ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc cgaacaataa 4560
agattctaca atactagctt ttatggttat gaagaggaaa aattggcagt aacctggccc 4620
cacaaacctt caaatcaacg aatcaaatta acaaccatag gataataatg cgattagttt 4680
tttagcctta tttctggggt aattaatcag cgaagcgatg atttttgatc tattaacaga 4740
tatataaatg caaaagctgc ataaccactt taactaatac tttcaacatt ttcggtttgt 4800
attacttctt attcaaatgt cataaaagta tcaacaaaaa attgttaata tacctctata 4860
ctttaacgtc aaggagaaaa aactataacg ctgacatggt ttctttaggt ttgatgaggc 4920
cgtcttttgt tgatagcagc tttttccatt tttttttttt ttgtttcgag taacgtatgg 4980
tttagtatct gtcttctcct tctcttacaa aaaaaccctt tgtaaaatag tgccgagttg 5040
gaggacatca atctgatggg caagaaaaca ccaacccccc ctatatgaaa agaaaatgat 5100
aagcagatag ataaaaatac ttaattaact aatacataaa aataagaggt atataaaaat 5160
attatatgga agcaataatt attactcttt atgaaaataa agaccaccaa ggcttccaag 5220
gtgtcaagtg atgttcgtga tgtctaccga aatggaaaca ggtcaacaat gataaaacag 5280
gaccgaatgg agatgttcta gcgttatgat tatcaggaaa ggcgtcatcg gtgtgtctat 5340
gtggcaacca tgtaccgaaa gtgaacaatt gtaaggcaga caacaatgca ggagcggccc 5400
aaaagactaa caaatttggc attctagcac ccaatattaa tacggcgact gcaactaata 5460
cagtcaaaac tgctaattct ctccaaccga agtaggttct aaagaaacca tagaaccaag 5520
gcaaaaatgc tcttggagcg tcggcatgaa aatcagggtc atctgctgta cctggtgcag 5580
cgtgatgagc gtgatgggca gttttcaatg gggtgaatct aaaaccggca tacaatgcta 5640
aagccaaaga accgattgct gtgtttaatc taggtctggc tggtgccaaa ctaccgtgca 5700
ttgcatcatg agcaactatg aataaaccta cggacaacca agtttgacca gctacaatta 5760
atgggacggt caaaacagac cagattgtcc atctgtggaa gtagacaccg tatatatgca 5820
atgtcaacca agcagcagca atcaaacctg ctaaagtcaa accaatcaat gcttgattag 5880
ggacaactct actcattgga gtaacagcgg acattttgaa tatgtattac ttggttatgg 5940
ttatatatga caaaagaaaa agaagaacag aagaataacg caaggaagaa caataactga 6000
aattgataga gaagtattat gtctttgtct ttttataata aatcaagtgc agaaatccgt 6060
tagacaacat gagggataaa atttaacgtg ggcgaagaag aaggaaaaaa gtttttgtga 6120
gggcgtaatt gaagcgatct gttgattgta gatttttttt ttttgaggag tcaaagtcag 6180
aagagaacag acaaatggta ttaaccatcc aatacttttt tggagcaacg ctaagctcat 6240
gcttttccat tggttacgtg ctcagttgtt agatatggaa agagaggatg ctcacggcag 6300
cgtgactcca attgagcccg aaagagagga tgccacgttt tcccgacggc tgctagaatg 6360
gaaaaaggaa aaatagaaga atcccattcc tatcattatt tacgtaatga cccacacatt 6420
tttgagattt tcaactatta cgtattacga taatcctgct gtcattatca ttattatcta 6480
tatcgacgta tgcaacgtat gtgaagccaa gtaggcaatt atttagtact gtcagtattg 6540
ttattcattt cttagggctt gcgtcagctt gatgaggatt tgtgatagta gccgttggag 6600
gcatgaaggg cattggaaac atggggaaag gaggtatacc gaaaggtaac ggtactggtg 6660
gcactggcgt accgccgtta tttacagaag ttggaaggct ggtattgttg ttcaagccag 6720
cggtgccagt tggagcgaaa agccaattag tgtgatacta agtgctttat cgaaaatccg 6780
tgatgccggt ccttcaggca tcaaatttca gtggcctaat tattcacaga gttctcatgt 6840
gacaagtatt gatgatagta gtgtcagtta tgcttcaggt tatgttacta taggataatg 6900
atcacggcta aaacggtcga atgtaagcat atatctttcg attgtataat tgttcccaaa 6960
tactacagca tctcaaggaa aaaaaaacaa aaacttccaa aaaaatcgaa tccctgagga 7020
atctttaata cattttcaat ctatttaagt tttataaacg tgtatatgag atgtcatgag 7080
catgaattat taataataaa aactaaatca ttaaagtaac ttaaggagtt aaatttatga 7140
aaataaagac caccaaggct tccaaggtgt caagtgatgt tcgtgatgtc taccgaaatg 7200
gaaacaggtc aacaatgata aaacaggacc gaatggagat gttctagcgt tatgattatc 7260
aggaaaggcg tcatcggtgt gtctatgtgg caaccatgta ccgaaagtga acaattgtaa 7320
ggcagacaac aatgcaggag cggcccaaaa gactaacaaa tttggcattc tagcacccaa 7380
tattaatacg gcgactgcaa ctaatacagt caaaactgct aattctctcc aaccgaagta 7440
ggttctaaag aaaccataga accaaggcaa aaatgctctt ggagcgtcgg catgaaaatc 7500
agggtcatct gctgtacctg gtgcagcgtg atgagcgtga tgggcagttt tcaatggggt 7560
gaatctaaaa ccggcataca atgctaaagc caaagaaccg attgctgtgt ttaatctagg 7620
tctggctggt gccaaactac cgtgcattgc atcatgagca actatgaata aacctacgga 7680
caaccaagtt tgaccagcta caattaatgg gacggtcaaa acagaccaga ttgtccatct 7740
gtggaagtag acaccgtata tatgcaatgt caaccaagca gcagcaatca aacctgctaa 7800
agtcaaacca atcaatgctt gattagggac aactctactc attggagtaa cagcggacat 7860
tttgtttgtt tatgtgtgtt tattcgaaac taagttcttg gtgttttaaa actaaaaaaa 7920
agactaacta taaaagtaga atttaagaag tttaagaaat agatttacag aattacaatc 7980
aatacctacc gtctttatat acttattagt caagtagggg aataatttca gggaactggt 8040
ttcaaccttt tttttcagct ttttccaaat cagagagagc agaaggtaat agaaggtgta 8100
agaaaatgag atagatacat gcgtgggtca attgccttgt gtcatcattt actccaggca 8160
ggttgcatca ctccattgag gttgtgcccg ttttttgcct gtttgtgccc ctgttctctg 8220
tagttgcgct aagagaatgg acctatgaac tgatggttgg tgaagaaaac aatattttgg 8280
tgctgggatt cttttttttt ctggatgcca gcttaaaaag cgggctccat tatatttagt 8340
ggatgccagg aataaactgt tcacccagac acctacgatg ttatatattc tgtgtaaccc 8400
gccccctatt ttgggcatgt acgggttaca gcagaattaa aaggctaatt ttttgactaa 8460
ataaagttag gaaaatcact actattaatt atttacgtat tctttgaaat ggcagtattg 8520
ataatgataa actcgaactg agagtgtaaa ctgcgaagct tgcaaattaa agccttcgag 8580
cgtcccaaaa ccttctcaag caaggttttc agtataatgt tacatgcgta cacgcgtttg 8640
tacagaaaaa aaagaaaaat ttgaaatata aataacgttc ttaatactaa cataactatt 8700
aaaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag 8760
cggatgtggg aggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa 8820
aggaaaaggg gcctgtttta tgaaaataaa gaccaccaag gcttccaagg tgtcaagtga 8880
tgttcgtgat gtctaccgaa atggaaacag gtcaacaatg ataaaacagg accgaatgga 8940
gatgttctag cgttatgatt atcaggaaag gcgtcatcgg tgtgtctatg tggcaaccat 9000
gtaccgaaag tgaacaattg taaggcagac aacaatgcag gagcggccca aaagactaac 9060
aaatttggca ttctagcacc caatattaat acggcgactg caactaatac agtcaaaact 9120
gctaattctc tccaaccgaa gtaggttcta aagaaaccat agaaccaagg caaaaatgct 9180
cttggagcgt cggcatgaaa atcagggtca tctgctgtac ctggtgcagc gtgatgagcg 9240
tgatgggcag ttttcaatgg ggtgaatcta aaaccggcat acaatgctaa agccaaagaa 9300
ccgattgctg tgtttaatct aggtctggct ggtgccaaac taccgtgcat tgcatcatga 9360
gcaactatga ataaacctac ggacaaccaa gtttgaccag ctacaattaa tgggacggtc 9420
aaaacagacc agattgtcca tctgtggaag tagacaccgt atatatgcaa tgtcaaccaa 9480
gcagcagcaa tcaaacctgc taaagtcaaa ccaatcaatg cttgattagg gacaactcta 9540
ctcattggag taacagcgga catttttttg attaaaatta aaaaaacttt ttgtttttgt 9600
gtttattctt tgttcttaga aaagacaagt tgagcttgtt tgttcttgat gttttattat 9660
tttacaatag ctgcaaatga agaatagatt cgaacattgt gaagtattgg catatatcgt 9720
ctctatttat actttttttt tttcagttct agtatatttt gtattttcct ccttttcatt 9780
ctttcagttg ccaataagtt acaggggatc tcgaaagatg gtggggattt ttccttgaaa 9840
gacgactttt tgccatctaa tttttccttg ttgcctctga aaattatcca gcagaagcaa 9900
atgtaaaaga tgaacctcag aagaacacgc aggggcccga aattgttcct acgagaagta 9960
gtgggtcata aaaagtttat tccctggaaa aaaaattttg cgttgccttt ctggagaatt 10020
ttttcgaatt agcgtgctgc cactgcatgc atttctgaga agtgtgggca ttcttccacc 10080
agttgttcct cctaaaaaaa aaaaagattt cctaccccgc acaaattcct gcatacccct 10140
catttccacg gggtaaaaaa aaaacatccc gtagcagcct ctgacggagc cgtatggaac 10200
aatcctggat aaatatggcg gaaaaacggc tctggctgcg gctttgttta aggcagaaac 10260
tagaggatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc 10320
tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa 10380
attgttatcc gctcacaatt ccacacaaca taggagccgg aagcataaag tgtaaagcct 10440
ggggtgccta atgagtgagg taactcacat taattgcgtt gcgctcactg cccgctttcc 10500
agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 10560
gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 10620
ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 10680
gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 10740
aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 10800
gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 10860
ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 10920
cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 10980
cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 11040
gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 11100
cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 11160
agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 11220
ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 11280
ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 11340
gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 11400
cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 11460
attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 11520
accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 11580
ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 11640
gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 11700
agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 11760
ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 11820
ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 11880
gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 11940
ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 12000
tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 12060
tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 12120
cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 12180
tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 12240
gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 12300
tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 12360
ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 12420
attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 12480
cgcgcacatt tccccgaaaa gtgccacctg aacgaagcat ctgtgcttca ttttgtagaa 12540
caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca 12600
gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg 12660
taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt 12720
ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt 12780
ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta 12840
ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg 12900
tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct 12960
gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata 13020
aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag 13080
tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc 13140
tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga 13200
atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg 13260
tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg 13320
gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta 13380
ttcgcaatat tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt 13440
cttcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagagaatag 13500
gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg 13560
cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat 13620
atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg 13680
cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat 13740
gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc 13800
tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatactaa 13860
gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 13920
c 13921
<210> 74
<211> 4669
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 74
ttacagtctt tgtagataat gaatctgacc atctaaattt tttgtacaga aaaaaaagaa 60
aaatttgaaa tataaataac gttcttaata ctaacataac tattaaaaaa aataaatagg 120
gacctagact tcaggttgtc taactccttc cttttcggtt agagcggatg tgggaggagg 180
gcgtgaatgt aagcgtgaca taactaatta catgatatcg acaaaggaaa aggggcctgt 240
tttatatcct aatatcgtta gagttctgtc cttggaagac gtttggcaac aactgattaa 300
taaaagatgc tggagtagta gtgtcgtccc taggaaagaa atagaagaac aagaaagtga 360
caaagtagca tgctaagcag taataaatcc agtgtgactc agtcttcctt gtttgctcag 420
gtgcatattt cttctgagag tcctgtaact tcctaggcaa agggttttgt ccgaatgact 480
tacagacctg gtcagaagtt aatttagatc ctgccaagac tattgggact cctgtaccag 540
ggtgagttga tgcaccgacg aaaaataaat tgtcatatct gttagtagag tcctttgttg 600
aaggtctgaa ccacaaaacc tgaaagacat catgtgataa acctaatatt gatcccctcc 660
acaagttgaa cttagattgc cagactgatg ggtcgttgac ctcctcgtgt tctatcaagt 720
tagcgaagtt gttgactccc aacctcctct ctatgacctc caagaccatc tttctagccc 780
tgttgaccaa ctctgggtag ttctcctctg ctgagttacc tgtcttagac ttcatgtgac 840
caattggaac taaaactata attgagtcct tatttggagg tgctgcagac tcgtctatcc 900
ttgaaggaac gttgacgtag aaagatgctt cagaaggcaa accgaaatcg ttaaaaatct 960
cgtcgaatga ctccttgtaa gcctcagcca agaaaatgtt gtggacgtcc aattgaggga 1020
ccttagtaga cattgaccag taaaaagaaa ttgatgaaga tgttaatttc ttagatgcca 1080
aagtcttctt tgtccagttg caaggtggca acaagtggtg gtaagcgtag accaagtcag 1140
cgttgcagac gacagcgtct gcctcaatga cttctccaga ctccaaagtg acaccagtga 1200
ccctcttgtc tttatcgaca gtgttaatct tagcgacagg agattggtac ctgaactcag 1260
ctccgtactt cttagaagct atagactcca acttttggac gaccatgttg aaaccacccc 1320
ttgggtacca aattccctct gcgaactctg tatattgcaa caatgagtag actgcaggtg 1380
cgtcgtaagg tgacataccc atgtacattg tttgaaaagt aaaagccatc ctcatctttt 1440
ttgtttggaa gtattttgat gctctgtcgt atatcttacc gaataagtgc aacctaaaaa 1500
tctctgggac gtactgtaac cttattaagt cccatatagt ttcgaagttt ctctttatag 1560
caatgaatgt accctgctcg tagtggacgt gtgtctcttt catgaaatct aagaacctac 1620
cgaatcctaa aggtccctca atcctgtcca actcaccctt catctttgtt aagtctgatg 1680
acaattggac agcgtcaccg tcgtcgaaat ggactttgta attgttgtca catcttaata 1740
agtccaagtg gtctcctatc ctctcgtcta agtcagcgaa agcgtcctca aacaacttag 1800
gcatcaagta caatgaaggt ccctggtcga acctgtgacc gtcgtggtga atgaaagagc 1860
accttcctcc agagaagtcg ttcttctcga cgacagtgac tctgaaaccc tcccttgcta 1920
accttgctgc tgttgcagta cctcctattc cagcacctat gacgacaatg tgcttcttct 1980
gatcagacat ttttttgatt aaaattaaaa aaactttttg tttttgtgtt tattctttgt 2040
tcttagaaaa gacaagttga gcttgtttgt tcttgatgtt ttattatttt acaatagctg 2100
caaatgaaga atagattcga acattgtgaa gtattggcat atatcgtctc tatttatact 2160
tttttttttt cagttctagt atattttgta ttttcctcct tttcattctt tcagttgcca 2220
ataagttaca ggggatctcg aaagatggtg gggatttttc cttgaaagac gactttttgc 2280
catctaattt ttccttgttg cctctgaaaa ttatccagca gaagcaaatg taaaagatga 2340
acctcagaag aacacgcagg ggcccgaaat tgttcctacg agaagtagtg ggtcataaaa 2400
agtttattcc ctggaaaaaa aattttgcgt tgcctttctg gagaattttt tcgaattagc 2460
gtgctgccac tgcatgcatt tctgagaagt gtgggcattc ttccaccagt tgttcctcct 2520
aaaaaaaaaa aagatttcct accccgcaca aattcctgca tacccctcat ttccacgggg 2580
taaaaaaaaa acatcccgta gcagcctctg acggagccgt atggaacaat cctggataaa 2640
tatggcggaa aaacggctct ggctgcggct ttgtttaagg cagaaactag acagttcgag 2700
tttatcatta tcaatactgc catttcaaag aatacgtaaa taattaatag tagtgatttt 2760
cctaacttta tttagtcaaa aaattagcct tttaattctg ctgtaacccg tacatgccca 2820
aaataggggg cgggttacac agaatatata acatcgtagg tgtctgggtg aacagtttat 2880
tcctggcatc cactaaatat aatggagccc gctttttaag ctggcatcca gaaaaaaaaa 2940
gaatcccagc accaaaatat tgttttcttc accaaccatc agttcatagg tccattctct 3000
tagcgcaact acagagaaca ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga 3060
gtgatgcaac ctgcctggag taaatgatga cacaaggcaa ttgacccacg catgtatcta 3120
tctcattttc ttacaccttc tattaccttc tgctctctct gatttggaaa aagctgaaaa 3180
aaaaggttga aaccagttcc ctgaaattat tcccctactt gactaataag tatataaaga 3240
cggtaggtat tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt 3300
atagttagtc ttttttttag ttttaaaaca ccaagaactt agtttcgaat aaacacacat 3360
aaacaaacaa aatgtcacaa ccaccattat tggaccacgc tacacaaact atggcaaacg 3420
gttctaaatc tttcgctact gctgctaaat tattcgaccc agcaacaaga agatctgtat 3480
tgatgttgta cacctggtgt agacattgcg atgacgttat agatgaccaa actcacggtt 3540
ttgcttcaga agctgcagcc gaagaagaag ctacacaaag attggcaaga ttaagaactt 3600
tgacattagc tgcattcgaa ggtgccgaaa tgcaagatcc agcttttgcc gctttccaag 3660
aagttgcatt aacccatggt attactccta gaatggcttt ggatcactta gacggttttg 3720
caatggatgt cgcccaaaca agatacgtaa ccttcgaaga cactttaaga tattgttacc 3780
atgtcgccgg tgttgtcggt ttgatgatgg ctagagtaat gggtgttaga gatgaaagag 3840
ttttagatag agcatgtgac ttgggtttag ccttccaatt gacaaacata gctagagata 3900
taatagatga cgcagccata gacagatgct atttgccagc tgaatggtta caagatgcag 3960
gtttgactcc tgaaaattac gctgcaagag aaaacagagc cgctttagcc agagttgctg 4020
aaagattgat agatgcagcc gaaccatatt acatctcttc acaagctggt ttgcatgatt 4080
tgccacctag atgcgcatgg gccattgcta ccgcaagatc tgtttacaga gaaatcggta 4140
ttaaagtcaa ggctgcaggt ggttccgcat gggatagaag acaacacact tctaaaggtg 4200
aaaagatcgc tatgttgatg gccgctcctg gtcaagttat tagagcaaag accaccagag 4260
tcaccccaag accagccggt ttatggcaaa gacctgttta aagctttgga cttcttcgcc 4320
agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac 4380
gaaaagatgg aaaagggtca aatcgttggt agatacgttg ttgacacttc taaataagcg 4440
aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac 4500
aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc 4560
ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct 4620
accggcatgc tctaaaccag cattgggcag ctgtctatat gaattataa 4669
<210> 75
<211> 4533
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 75
atagaattgg attatgtaaa aggtgaagat accattgtag tttgtacaga aaaaaaagaa 60
aaatttgaaa tataaataac gttcttaata ctaacataac tattaaaaaa aataaatagg 120
gacctagact tcaggttgtc taactccttc cttttcggtt agagcggatg tgggaggagg 180
gcgtgaatgt aagcgtgaca taactaatta catgatatcg acaaaggaaa aggggcctgt 240
tttaacgatg agtcgtcata atggcttgca atgctgctaa taccggaaca ggcggcttgc 300
cgctcagaat acgtagccga tcggtcagcg tgagttttcc cgcataaaaa cgggcaatta 360
aatcttcagg taaaccataa aaacgctgca taacccgcca gcgtgaatcg gcgggtccgg 420
ctaaaaacag catgcgattc agcatgcgga aaaagcgctg ctgctgccag cgctcgcggg 480
caaaatgcgt aatggcctgg tgaattgagg ccgacgtaaa gacatcaagt gcgctcaggc 540
ggtcggccac ggcaaccgcc agcggcagtg aatagccggt ggtaggatgg aacagaccgg 600
cacgtaatcc actacaggcc agggggcgct gctgccagaa tgcgtcggca ttgcccgaca 660
gggtgatggg taaggcgccc tgttcttcac gcagcaatgt ctgaagctgc caaccctgtt 720
gcgcggcata gtcgcaaata ttttgccgcg cgcgttcagg atctaatgtc gcattatcga 780
tatagtgcgt gtcttcaatt aacaatctgg tcggcgagag cggcaggctg tacacgaagc 840
gataaccatt ttgctgatcg accgtggcat ccatgataat gggagacgat aaaccatgcg 900
gttggctcaa tcgccattcc tggccaataa acgcctggaa gcccacgctc agtgctgagt 960
ttgccgcata accccgcccg tcaatcaccg cgcgtgcacc gataacctga ccctttttca 1020
accgaacaga ttccgcatta acctctgcga ccgcggtatc catccacaag tgcgggccaa 1080
actgtcgctg taaaacctca gcgaaacgct gagaagtaat acagaagtag ccgctgttca 1140
gcttacgacg gcgtgtggga aagcgcacct gatagtcggg ccagtgatga accaccagcg 1200
gagctatcca gcgatgttgg ctctcagtca aatcatcgtg gtgaaatgac cacgtatgat 1260
tcccgcccgc ctggggtgcg gcgtcgataa gcaaaatacg catatcaggt tgctgctgct 1320
gaagacgcag ggcgataagg ccattcgcga gtccagcccc cacgagaatc agatcataat 1380
gcggttgcat ttttttgatt aaaattaaaa aaactttttg tttttgtgtt tattctttgt 1440
tcttagaaaa gacaagttga gcttgtttgt tcttgatgtt ttattatttt acaatagctg 1500
caaatgaaga atagattcga acattgtgaa gtattggcat atatcgtctc tatttatact 1560
tttttttttt cagttctagt atattttgta ttttcctcct tttcattctt tcagttgcca 1620
ataagttaca ggggatctcg aaagatggtg gggatttttc cttgaaagac gactttttgc 1680
catctaattt ttccttgttg cctctgaaaa ttatccagca gaagcaaatg taaaagatga 1740
acctcagaag aacacgcagg ggcccgaaat tgttcctacg agaagtagtg ggtcataaaa 1800
agtttattcc ctggaaaaaa aattttgcgt tgcctttctg gagaattttt tcgaattagc 1860
gtgctgccac tgcatgcatt tctgagaagt gtgggcattc ttccaccagt tgttcctcct 1920
aaaaaaaaaa aagatttcct accccgcaca aattcctgca tacccctcat ttccacgggg 1980
taaaaaaaaa acatcccgta gcagcctctg acggagccgt atggaacaat cctggataaa 2040
tatggcggaa aaacggctct ggctgcggct ttgtttaagg cagaaactag atccaactgg 2100
caccgctggc ttgaacaaca ataccagcct tccaacttct gtaaataacg gcggtacgcc 2160
agtgccacca gtaccgttac ctttcggtat acctcctttc cccatgtttc caatgccctt 2220
catgcctcca acggctacta tcacaaatcc tcatcaagct gacgcaagcc ctaagaaatg 2280
aataacaata ctgacagtac taaataattg cctacttggc ttcacatacg ttgcatacgt 2340
cgatatagat aataatgata atgacagcag gattatcgta atacgtaata gttgaaaatc 2400
tcaaaaatgt gtgggtcatt acgtaaataa tgataggaat gggattcttc tatttttcct 2460
ttttccattc tagcagccgt cgggaaaacg tggcatcctc tctttcgggc tcaattggag 2520
tcacgctgcc gtgagcatcc tctctttcca tatctaacaa ctgagcacgt aaccaatgga 2580
aaagcatgag cttagcgttg ctccaaaaaa gtattggatg gttaatacca tttgtctgtt 2640
ctcttctgac tttgactcct caaaaaaaaa aaatctacaa tcaacagatc gcttcaatta 2700
cgccctcaca aaaacttttt tccttcttct tcgcccacgt taaattttat ccctcatgtt 2760
gtctaacgga tttctgcact tgatttatta taaaaagaca aagacataat acttctctat 2820
caatttcagt tattgttctt ccttgcgtta ttcttctgtt cttctttttc ttttgtcata 2880
tataaccata accaagtaat acatattcaa aatggcttat accgcaatgg cagcaggaac 2940
tcagtcattg cagttgagga cagtcgcctc ttaccaggag tgcaactcaa tgaggtcttg 3000
cttcaagttg accccattca agtcattcca cggtgtcaac ttcaacgttc cttctttagg 3060
tgccgccaac tgcgaaatca tgggtcactt gaaattgggt tctttgccat acaaacagtg 3120
ttcagtatca tctaagtcaa ctaagactat ggcccagttg gtagatttgg cagagaccga 3180
gaaagccgag ggaaaggata tcgagttcga ttttaacgag tatatgaagt ctaaggctgt 3240
cgctgttgat gcagccttgg ataaggccat ccctttggag tatccagaga agatccatga 3300
gtctatgagg tactcattgt tggccggagg aaaaagggtc agacctgcat tatgcatcgc 3360
tgcttgcgag ttagtaggtg gttctcagga cttggccatg ccaaccgcat gtgccatgga 3420
aatgattcat accatgtcat tgattcacga tgatttgcct tgcatggaca acgacgactt 3480
cagaagggga aagcctacca atcacaaggt tttcggagag gacactgctg ttttagccgg 3540
tgacgcattg ttatctttcg cttttgaaca catcgccgtt gccacatcaa aaactgtccc 3600
atctgacagg accttgagag tcatttctga gttgggtaaa accatcggtt cacagggatt 3660
ggtcggaggt caggtagtcg acatcacttc tgagggagac gccaacgtcg acttaaagac 3720
attggagtgg attcacattc acaagactgc cgtcttgttg gaatgctctg ttgtttctgg 3780
aggaatcttg ggtggagcta ccgaggatga gattgctaga ataagaagat acgccaggtg 3840
cgtcggtttg ttgttccagg ttgtcgacga cattttggat gtcaccaagt cttcagagga 3900
attgggaaag accgccggta aagacttatt gaccgacaag gctacctacc ctaagttgat 3960
gggtttggag aaggccaaag agtttgcagc agaattagct accagggcaa aggaagagtt 4020
gtcatcattc gaccagatca aggcagcccc tttgttagga ttggccgatt acatcgcttt 4080
caggcaaaac taaatttaac tccttaagtt actttaatga tttagttttt attattaata 4140
attcatgctc atgacatctc atatacacgt ttataaaact taaatagatt gaaaatgtat 4200
taaagattcc tcagggattc gatttttttg gaagtttttg tttttttttc cttgagatgc 4260
tgtagtattt gggaacaatt atacaatcga aagatatatg cttacattcg accgttttag 4320
ccgtgatcat tatcctatag taacataacc tgaagcataa ctgacactac tatcatcaat 4380
acttgtcaca tgagaactct gtgaataatt aggccactga aatttgatgc ctgaaggacc 4440
ggcatcacgg attttcgata aagcacttag tatcacacta attggctttt cgcattgatc 4500
tcctcttggg aacggtgagt gcaacgaatg cga 4533

Claims (10)

1.一株重组酿酒酵母，其组成型表达β-胡萝卜素酮化酶基因CrtW，诱导型表达β-胡萝卜素羟化酶基因CrtZ，将中间产物β-胡萝卜素合成为虾青素，所述β-胡萝卜素酮化酶基因CrtW和β-胡萝卜素羟化酶基因CrtZ的拷贝数之比为3:1-3:2。
2.根据权利要求1所述的重组酿酒酵母，其特征在于：所述β-胡萝卜素酮化酶基因CrtW和β-胡萝卜素羟化酶基因CrtZ的拷贝数之比为3:2。
3.根据权利要求1或2所述的重组酿酒酵母，其特征在于：所述组成型表达β-胡萝卜素酮化酶基因CrtW所用的组成型启动子为TDH3p、FBA1p和HXT7p中的一种或多种；和/或
所述诱导型表达β-胡萝卜素羟化酶基因CrtZ所用的诱导型启动子为Gal1和Gal10中的一种或多种。
4.一种酿酒酵母的重组表达载体，包括组成型启动子及其控制下的β-胡萝卜素酮化酶基因CrtW，以及诱导型启动子及其控制下的β-胡萝卜素羟化酶基因CrtZ，所述β-胡萝卜素酮化酶基因CrtW和β-胡萝卜素羟化酶基因CrtZ的拷贝数之比为3:1-3:2。
5.根据权利要求4所述的重组表达载体，其特征在于：所述组成型启动子为TDH3p、FBA1p和HXT7p中的一种或多种；和/或
所述诱导型启动子为Gal1和Gal10中的一种或多种。
6.根据权利要求4或5所述的重组表达载体，其特征在于：所述重组表达载体为SEQ IDNO：58所示的PRS426-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t、SEQ IDNO：67所示的PRS426-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t和SEQ ID NO：73所示的PRS426-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-Gal10-CrtZ-TPI1t中的一种或多种，优选为PRS426-HXT7p-CrtW-CYC1t-TDH3p-CrtW-TDH2t-FBA1p-CrtW-TEF2t-Gal1-CrtZ-ADH1t-Gal10-CrtZ-TPI1t。
7.一种构建权利要求1-3任一项所述的重组酿酒酵母的方法，包括将权利要求4-6任一项所述的重组表达载体转入出发酿酒酵母中获得重组酿酒酵母的步骤；
所述出发酿酒酵母为能合成β-胡萝卜素的酿酒酵母。
8.一种生产虾青素的方法，包括将权利要求1-3任一项所述的重组酿酒酵母进行发酵培养，使得β-胡萝卜素酮化酶基因CrtW组成型表达并且在发酵培养过程中添加D-(+)-半乳糖诱导β-胡萝卜素羟化酶基因CrtZ表达以合成虾青素的步骤。
9.根据权利要求8所述的方法，其特征在于：所述发酵培养包括将权利要求1-3任一项所述的重组酿酒酵母在发酵培养基中在20℃-30℃进行发酵培养，在发酵的第30-54小时加入D-(+)-半乳糖；
所述发酵培养的温度优选为20℃；
所述D-(+)-半乳糖优选在发酵的第36小时加入。
10.权利要求1-3任一项所述的重组酿酒酵母和/或权利要求4-6任一项所述的重组表达载体在生产虾青素中的应用。
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