CN113694090A - Ginseng and pilose antler three-kidney composition and preparation method thereof - Google Patents
Ginseng and pilose antler three-kidney composition and preparation method thereof Download PDFInfo
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Abstract
The application relates to the field of traditional Chinese medicine preparation, and particularly discloses a ginseng-pilose antler-three-kidney composition and a preparation method thereof. The preparation method comprises the following steps: s1, respectively soaking the segmented ox kidney, donkey kidney and dog kidney in yellow wine, and sterilizing and drying for later use; s2, pulverizing dried ren bovis seu Bubali, ren donkey, testis et Pentis Canis, radix Ginseng and cornu Cervi Pantotrichum, sieving, and collecting the sieved powder; s3, mixing the mixed powder for 15-25min, and sieving to obtain Ginseng and cornu Cervi Pantotrichum three-kidney composition. The ginseng-pilose antler three-kidney composition can be used for treating diseases such as impotence due to kidney deficiency, easy fatigue, tinnitus, spontaneous perspiration and the like, and has the advantages of good effect of tonifying yang and kidney and quick curative effect.
Description
Technical Field
The application relates to the technical field of traditional Chinese medicine preparation, in particular to a ginseng-pilose antler three-kidney composition and a preparation method thereof.
Background
At present, a plurality of medicines for tonifying yang and kidney and treating waist soreness and leg weakness and fatigue are available on the market, and the medicines mainly comprise western medicines and traditional Chinese medicines. Although the western medicines have quick drug effects, the western medicines only have a certain effect of relieving symptoms in a short period, only treat symptoms of kidney deficiency and impotence, and simultaneously easily cause patients to gradually lose the original self-regulation capacity of the patients to form drug dependence, so that the problem is difficult to fundamentally solve. The traditional Chinese medicine in the related technology is based on the premise of solving the problems of invigorating yang and tonifying kidney, and simultaneously takes care of regulating various organs such as heart, liver, spleen, lung and the like to regulate the whole function of the human body to fulfill the aim of invigorating the kidney and tonifying yang.
In the compositions for tonifying yang and invigorating kidney in the related art, a plurality of traditional Chinese medicines are mostly used as main health-care and functional raw materials, and are compounded with different auxiliary materials and then processed to obtain corresponding products, so as to realize better health-care efficacy. In these compositions for tonifying kidney and strengthening yang mentioned in the related art, extracts are obtained by subjecting various Chinese herbal medicines to extraction, etc., and then the extracts are further processed to obtain the compositions for tonifying kidney and strengthening yang. The composition for tonifying kidney and strengthening yang obtained by the method is used for treating symptoms such as impotence, premature ejaculation, easy fatigue, kidney deficiency, tinnitus, spontaneous perspiration and the like, and has poor comprehensive treatment effect; secondly, the treatment period is long, generally about 1 week of a treatment course, and 2-3 treatment courses are needed.
Disclosure of Invention
In order to solve the problem of poor comprehensive treatment effect of related yang-tonifying and kidney-tonifying medicines in treating impotence due to kidney deficiency and easy fatigue, the application provides a ginseng-antler three-kidney composition and a preparation method thereof.
In a first aspect, the application provides a preparation method of a ginseng-pilose antler three-kidney composition, which adopts the following technical scheme:
a preparation method of a ginseng-pilose antler-three-kidney composition comprises the following steps:
s1, respectively soaking the segmented ox kidney, donkey kidney and dog kidney in yellow wine, sterilizing and drying for later use;
s2, mixing and crushing the dried ox kidney, donkey kidney and dog kidney, sun-dried ginseng and hairy antler, and sieving by a 100-mesh sieve to obtain undersize products, namely mixed powder; s3, mixing the mixed powder for 15-25min, and then sieving with a 100-mesh sieve to obtain the ginseng antler and three-kidney composition.
By adopting the technical scheme, the Chinese medicine is named as bovine kidney. Sweet, salty and neutral in flavor, and has the effects of tonifying kidney, replenishing vital essence, strengthening waist and knees and relieving arthralgia; it can be used for treating asthenia, kidney deficiency, sexual impotence, qi deficiency, soreness of waist and knees, and arthralgia due to dampness. In addition, the beef kidney is rich in various nutrients, especially the content of protein and vitamin, so as to supplement the nutrition required by the human body; the ox kidney has good effect of tonifying the body, can effectively tonify the kidney and qi for people who are weak and often have tinnitus and dizziness by eating some ox kidneys, and has good effect on improving the weak and weak body and excessive sweating.
Every 100g of the beef kidney contains 82g of water, 12.8g of protein, 0.3g of carbohydrate, 3.7g of fat and 0.9g of ash; 17mg of calcium, 198mg of phosphorus, 11.4mg of iron, 0.34mg of thiamine, 1.75mg of riboflavin, 5.1mg of nicotinic acid, vitamin, C6 mg and 340ug of vitamin A. Besides the nutrient substances, the adrenal cortex of the ox kidney can secrete a plurality of steroid hormones including grain corticoids (glucocorticoids), mineralocorticoids (mineral corticoids) and adrenal medulla, and has good effects of tonifying yang and tonifying kidney.
Dog kidney, a Chinese herbal medicine, has mild smell and mild nature; alias: penis et testis Canitis, testis et penis Canitis, contains androgen, protein, fat, and further contains 17 kinds of amino acids; has effects in warming kidney, tonifying yang, and replenishing vital essence, and can be used for treating kidney yang asthenia, sexual impotence, spermatorrhea, and weakness of waist and knees. The record of the materia medica shiyi includes: puerperal women who have postpartum kidney fatigue as malaria; the record of Yunnan herbal medicine: has mild smell.
Donkey kidney, named as a traditional Chinese medicine, has the effects of tonifying kidney and strengthening yang, nourishing yin and tonifying deficiency, and strengthening tendons and bones; it can be used for treating sexual impotence, blood deficiency, qi deficiency, bone tuberculosis, osteomyelitis, and female breast milk deficiency.
The sun-dried ginseng is a commercial ginseng, is a product obtained by digging out roots of ginseng, cleaning and drying in the sun, contains ginsenoside (ginseng saponins) which is a main active ingredient of the ginseng, and has the effects of improving immunity, relieving fatigue, accelerating organism recovery and the like.
The hairy antler is a rare traditional Chinese medicine. The cornu Cervi Pantotrichum contains phospholipid, glycolipid, gum lipid, hormone, fatty acid, amino acid, protein, and calcium, phosphorus, magnesium, and sodium, wherein the amino acid component accounts for more than half of the total components. Ancient physicians thought that the essence of deer is in the horn, antler is the tender shoot of the horn, and the qi is full without excretion, so the most powerful of tonifying yang and nourishing blood. Cornu Cervi Pantotrichum is warm in nature but not dry, and has effects of exciting and improving organism function, and can be used for treating general weakness and long-term illness. Li Shizhen is called pilose antler from Ben Cao gang mu as good at tonifying kidney and strengthening yang, producing essence and replenishing blood, and tonifying marrow and strengthening bone, but it can only be combined with ginseng to obtain good effect.
According to the scheme, the three kidneys (the ox kidney, the dog kidney and the donkey kidney) are treated by a specific method, and then the three kidneys, the sun-dried ginseng and the yellow hairy antler are mixed and treated according to a specific proportion, so that the ginseng-antler three-kidney composition with the effects of efficiently tonifying yang and kidney, reducing fatigue and supplementing kidney yang is prepared.
Specifically, according to the method, the three kidney raw materials (the beef kidney, the dog kidney and the donkey kidney) are cut into segments and are soaked in yellow wine in a sealing mode, so that the drug effects of tonifying yang and tonifying kidney and improving fatigue resistance of the beef kidney, the dog kidney and the donkey kidney can be effectively improved, and the effects of removing odor and fishy smell are achieved. In the application, the yellow wine is not added to extract the components with medicinal effects in the ox kidney, the dog kidney and the donkey kidney, but functional substances with the effects of tonifying yang and kidney and the like are retained in the ox kidney, the dog kidney and the donkey kidney, and the medicinal effects of tonifying yang and kidney and resisting fatigue of the ox kidney, the dog kidney or the donkey kidney are further improved by sealed impregnation. The sun-dried ginseng and the hairy antler are mixed for use, so that the effects of tonifying kidney and strengthening yang, generating sperm and replenishing blood, and replenishing marrow and strengthening bone of the hairy antler can be further improved, the sun-dried ginseng and the hairy antler are matched with three kidneys (bovine kidney, donkey kidney and dog kidney) for use, so that the kidney yang and the kidney can be effectively supplemented, and the effect of better tonifying qi is realized while the yang and the kidney are strengthened, so that the ginseng-pilose antler three-kidney composition has the effects of regulating the body function in multiple aspects, and comprehensively improving the problems of mental weakness, impotence and spermatorrhea, soreness of waist and legs and tinnitus spontaneous perspiration caused by insufficiency of kidney yang.
Preferably, the treatment process of the bovine kidney specifically comprises the following steps:
s1-1, cleaning the beef kidney and cutting into segments;
s1-2, soaking the beef kidney in yellow wine in a sealed manner for 45-90 hours, and turning over every 10-14 hours, wherein the addition amount of the yellow wine is 0.8-1.4 times of the weight of the beef kidney;
s1-3, disinfecting the beef kidney soaked with the yellow wine;
s1-4, drying the disinfected beef kidney in vacuum until the water content is lower than 4%.
Preferably, the donkey kidney treatment process specifically comprises the following steps:
s2-1, cleaning donkey kidney and cutting into segments;
s2-2, soaking donkey kidney in yellow wine in a sealed manner for 45-90h, and turning over every 10-14h, wherein the addition amount of the yellow wine is 0.8-1.4 times of the weight of the donkey kidney;
s2-3, disinfecting the donkey kidney soaked with yellow wine;
s2-4, drying the sterilized donkey kidney in vacuum until the water content is lower than 4%.
Preferably, the treatment process of the dog kidney specifically comprises the following steps:
s3-1, cleaning dog kidney, and cutting into segments;
s3-2, soaking the dog kidney in yellow wine, sealing and soaking for 45-90h, and turning over once every 10-14h, wherein the addition amount of the yellow wine is 0.8-1.4 times of the weight of the dog kidney;
s3-3, disinfecting the dog kidney soaked with yellow wine;
s3-4, drying the disinfected dog kidney in vacuum until the water content is lower than 4%.
By adopting the technical scheme, the efficacy of the beef kidney (or the dog kidney and the donkey kidney) can be effectively improved by soaking the beef kidney (or the dog kidney and the donkey kidney) with the yellow wine under the process conditions. When the three kidneys obtained by the preparation process are used for preparing the ginseng antler three-kidney composition, and the obtained ginseng antler three-kidney composition is used for mice, the composition has better effects of tonifying yang and tonifying the kidney on castrated mice: compared with the model control group, the weight of the seminal vesicle-prostate gland can be increased from 4.33mg/10g of the mouse body weight to 13.34mg/10g of the mouse body weight, which is close to the value of 15.26mg/10g of the mouse body weight of the normal control group, and the weight of the peridermal gland can be increased from 4.32mg/10g of the mouse body weight to 8.17mg/10g of the mouse body weight, which is close to the value of 8.51mg/10g of the mouse body weight of the normal control group. Meanwhile, the ginseng-antler-three-kidney composition can effectively improve the sexual function of mice, so that the quality of the accessory organs and the number of sperms of the mice are improved.
Preferably, the ginseng-antler-three-kidney powder is prepared from the following raw materials, by weight, 20-40 parts of sun-dried ginseng, 10-20 parts of yellow hairy antler, 45-75 parts of ox kidney, 75-105 parts of donkey kidney and 5-13 parts of dog kidney.
By adopting the technical scheme, in the ginseng-antler-three-kidney composition obtained by the raw material proportion, the ox kidney, the donkey kidney and the dog kidney are taken as main active pharmaceutical ingredients, so that the effects of strengthening yang, tonifying kidney and improving fatigue resistance are achieved; the pilose antler is matched with the drug effects of three kidneys to further promote the effects of tonifying kidney and strengthening yang, producing sperm and benefiting blood of the three kidneys, and is matched with the sun-dried ginseng to have the effects of tonifying kidney and qi, so that the prepared ginseng-pilose antler three-kidney composition has the effect of comprehensively conditioning the functions of a human body.
Preferably, the vacuum degree of the vacuum drying is (-0.02) - (-0.07) MPa, the drying time is 20-24h, and the drying temperature is 60-70 ℃.
In a second aspect, the application provides a ginseng and pilose antler three-kidney composition, which adopts the following technical scheme:
a composition containing Ginseng radix and cornu Cervi Pantotrichum for invigorating kidney is prepared by the above method.
Preferably, the composition is added with conventional auxiliary materials and prepared into one of clinically acceptable pills, powder, tablets, granules, capsules, microcapsules, paste, wine, syrup and oral liquid by a conventional process.
Preferably, the composition is prepared into powder or capsules.
By adopting the technical scheme, the ginseng-pilose antler-three-kidney composition can be prepared into medicines with different formulations by a conventional process, and is wide in application.
Preferably, when the composition is prepared into powder, the mixed composition is placed in a high-density polyethylene bottle for oral administration and medicine and sealed.
In summary, the present application has the following beneficial effects:
1. the application adopts yellow wine to soak the ox kidney, the donkey kidney and the dog kidney to improve the drug effect, and the treated ox kidney, donkey kidney and dog kidney are mixed with the sun-dried ginseng and the yellow hairy antler to prepare the ginseng-antler-three-kidney composition. The prepared ginseng-pilose antler three-kidney composition has obvious effects of tonifying yang, tonifying kidney and resisting fatigue, and has the effects of tonifying qi and essence and improving symptoms such as tinnitus, spontaneous perspiration and neurasthenia.
2. In the application, yellow wine with the quality of 0.8-1.4 times of the ox kidney (donkey kidney or dog kidney) is preferably adopted to soak the ox kidney (donkey kidney or dog kidney) for 45-90h, and compared with a non-soaking process, the efficacy of the ox kidney (donkey kidney or dog kidney) is effectively improved. When the ginseng-pilose antler three-kidney composition is used for mice, the weight of the seminal vesicle fluid-prostate of the castrated mice is increased by 30.02% (from the weight of 10.26mg/10g mice to 13.34mg/10g mice), and the weight of the glandula preputiales is increased by 23.23% (from the weight of 6.63mg/10g mice to 8.17mg/10g mice); the number of sperm in normal mice increased by 8.12% (from 652X 10)7The epididymis per gram is improved to 705 multiplied by 107Per gram epididymis); the anti-fatigue time of normal mice is prolonged by 16.8% (from 4.52min to 5.28 min).
3. When the ginseng-pilose antler three-kidney composition is administrated to corresponding patients, the cure rate in one treatment course (one week) reaches 54%, the cure rate after two treatment courses (two weeks) reaches 92%, and the treatment period is short.
Detailed Description
The present application will be described in further detail with reference to examples.
The ginseng-pilose antler three-kidney composition is prepared by taking three kidneys (including ox kidney, donkey kidney and dog kidney), sun-dried ginseng and yellow hairy antler as raw materials, processing the three kidneys with yellow wine respectively, mixing the processed three kidneys with the sun-dried ginseng and the yellow hairy antler, crushing the mixture, further mixing the crushed mixture and finally preparing the ginseng-pilose antler three-kidney composition. In the process, the three kidneys soaked by the yellow wine are taken as raw materials to be used as medicines; the yellow wine is not used for leaching out the ingredients with medicinal effects in the three kidneys, but is only used for effectively improving the medicinal effects of the three kidneys and removing part of fishy smell of the three kidneys. According to the application, the time for soaking the three kidneys with the yellow wine and the dosage proportion of the yellow wine to the three kidneys are adjusted, so that the effect of the three kidneys is effectively enhanced in a yellow wine soaking mode, and further, the composition with the effects of efficiently tonifying yang and kidney, tonifying qi and relieving fatigue is obtained.
The composition is added with conventional auxiliary materials and prepared into one of clinically acceptable pills, powder, tablets, granules, capsules, microcapsules, paste, medicinal liquor, syrup and oral liquid by a conventional process.
When the ginseng and pilose antler three-kidney composition is prepared into powder, the mixed composition is placed in an oral medicinal high-density polyethylene bottle and sealed, and the ginseng and pilose antler three-kidney powder can be obtained.
The starting materials in this application are all commercially available.
Preparation example of bovine Kidney
Preparation example 1
The treatment process of the bovine kidney specifically comprises the following steps:
s1-1, washing: placing the collected ox kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until the surface of the ox kidney is free of dust and stains, cutting the ox kidney into sections with the specification of 2-4mm, and placing the small sections of the ox kidney in the stainless steel barrel;
s1-2, dipping: pouring yellow wine 1 time of the weight of the beef kidney into a stainless steel barrel, soaking the beef kidney in the yellow wine, sealing and soaking for 72 hours, and turning over once every 12 hours;
s1-3, disinfection: loading the ox kidney soaked with yellow wine into a clean low-density polyethylene plastic bag, feeding the ox kidney into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol to perform cleaning and disinfection;
s1-4, drying: transferring the sterilized ren bovis Seu Bubali into a clean vacuum drying chamber, placing the ren bovis Seu Bubali in a vacuum drying oven, controlling the drying temperature at 65 deg.C and the vacuum degree at (-0.02) - (-0.07) MPa, and drying for 22h to obtain ren bovis Seu Bubali with water content of 3.56%.
Preparation example 2
The difference between the preparation example and the preparation example 1 is that the specific process parameters in the treatment process of the bovine kidney are different, and the treatment process of the bovine kidney specifically comprises the following steps:
s1-1, washing: placing the collected ox kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until the surface of the ox kidney is free of dust and stains, cutting the ox kidney into sections with the specification of 2-4mm, and placing the small sections of the ox kidney in the stainless steel barrel;
s1-2, dipping: pouring yellow wine with weight 0.8 times of that of the ox kidney into a stainless steel barrel to ensure that the ox kidney is soaked in the yellow wine, then sealing and soaking for 45 hours, and turning over once every 10 hours;
s1-3, disinfection: loading the ox kidney soaked with yellow wine into a clean low-density polyethylene plastic bag, feeding the ox kidney into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol to perform cleaning and disinfection;
s1-4, drying: transferring the sterilized ren bovis Seu Bubali into a clean vacuum drying chamber, placing ren bovis Seu Bubali in a vacuum drying oven, controlling drying temperature at 60 deg.C and vacuum degree at (-0.02) - (-0.07) MPa, and drying for 24 hr to obtain ren bovis Seu Bubali with water content of 3.37%.
Preparation example 3
The difference between the preparation example and the preparation example 1 is that the specific process parameters in the treatment process of the bovine kidney are different, and the treatment process of the bovine kidney specifically comprises the following steps:
s1-1, washing: placing the collected ox kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until the surface of the ox kidney is free of dust and stains, cutting the ox kidney into sections with the specification of 2-4mm, and placing the small sections of the ox kidney in the stainless steel barrel;
s1-2, dipping: pouring yellow wine 1.4 times of the weight of the beef kidney into a stainless steel barrel to ensure that the beef kidney is soaked in the yellow wine, then sealing and soaking for 90 hours, and turning over once every 14 hours;
s1-3, disinfection: loading the ox kidney soaked with yellow wine into a clean low-density polyethylene plastic bag, feeding the ox kidney into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol to perform cleaning and disinfection;
s1-4, drying: transferring the sterilized ren bovis Seu Bubali into a clean vacuum drying chamber, placing ren bovis Seu Bubali in a vacuum drying oven, controlling drying temperature at 70 deg.C and vacuum degree at (-0.02) - (-0.07) MPa, and drying for 20 hr to obtain ren bovis Seu Bubali with water content of 2.98%.
Preparation example 4
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating the beef kidney, and the beef kidney is soaked by the yellow wine with the weight 0.8 times of the weight of the beef kidney in the preparation example.
Preparation example 5
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating the beef kidney, and the beef kidney is soaked by the yellow wine with the weight 0.5 times of the weight of the beef kidney in the preparation example.
Preparation example 6
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating the beef kidney, and the beef kidney is soaked by the yellow wine with the weight 1.4 times of that of the beef kidney in the preparation example.
Preparation example 7
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating the beef kidney, and the beef kidney is soaked by the yellow wine with the weight 1.8 times of that of the beef kidney in the preparation example.
Preparation example 8
The difference between the preparation example and the preparation example 1 is that the beef kidney is soaked in yellow wine for 45 hours in the preparation example, but the time for soaking the beef kidney in the yellow wine is different in the process of treating the beef kidney.
Preparation example 9
The difference between the preparation example and the preparation example 1 is that the time for soaking the beef kidney with yellow wine is different in the process of treating the beef kidney, and the beef kidney is soaked with the yellow wine for 90 hours in the preparation example.
Preparation example 10
The difference between this preparation example and preparation example 1 is that the bovine kidney was not soaked with yellow wine during the treatment of the bovine kidney.
The treatment process of the bovine kidney specifically comprises the following steps:
s1-1, washing: placing the collected ox kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until the surface of the ox kidney is free of dust and stains, and cutting the ox kidney into sections with the specification of 2-4 mm;
s1-2, placing the small-section beef kidney in a clean low-density polyethylene plastic bag, feeding the bag into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol to perform cleaning and disinfection;
s1-3, drying, the steps are the same as the preparation example 1, and the beef kidney with the water content of 2.42 percent is obtained.
Preparation example of dog Kidney
Preparation example 1
The treatment process of the dog kidney specifically comprises the following steps:
s1-1, washing: placing the dog kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until no dust or stain is on the surface of the dog kidney, cutting the dog kidney into segments of 2-4mm, and then placing the segments in the stainless steel barrel;
s1-2, dipping: pouring yellow wine 1 time of weight of dog kidney into stainless steel barrel, soaking dog kidney in yellow wine, sealing and soaking for 72h, and turning over once every 12 h;
s1-3, disinfection: loading the penis et testis Canitis soaked with yellow wine into a clean low-density polyethylene plastic bag, feeding into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol for cleaning and disinfection;
s1-4, drying: transferring the sterilized dog kidney into a clean vacuum drying chamber, placing the dog kidney in a vacuum drying oven, controlling the drying temperature at 65 deg.C and the vacuum degree at (-0.02) - (-0.07) MPa, and drying for 21h to obtain dog kidney with water content of 2.13%.
Preparation example 2
The difference between the preparation example and the preparation example 1 is that the specific technological parameters in the treatment process of the dog kidney are different, and the treatment process of the dog kidney specifically comprises the following steps:
s1-1, washing: placing the dog kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until no dust or stain is on the surface of the dog kidney, cutting the dog kidney into segments of 2-4mm, and then placing the segments in the stainless steel barrel;
s1-2, dipping: pouring yellow wine with weight 0.8 times of dog kidney into stainless steel barrel, soaking dog kidney in yellow wine, sealing and soaking for 45 hr, and turning over once every 10 hr;
s1-3, disinfection: loading the penis et testis Canitis soaked with yellow wine into a clean low-density polyethylene plastic bag, feeding into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol for cleaning and disinfection;
s1-4, drying: transferring the sterilized dog kidney into a clean vacuum drying chamber, placing the dog kidney in a vacuum drying oven, controlling the drying temperature at 60 deg.C and the vacuum degree at (-0.02) - (-0.07) MPa, and drying for 24h to obtain dog kidney with water content of 2.37%.
Preparation example 3
The difference between the preparation example and the preparation example 1 is that the specific technological parameters in the treatment process of the dog kidney are different, and the treatment process of the dog kidney specifically comprises the following steps:
s1-1, washing: placing the dog kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until no dust or stain is on the surface of the dog kidney, cutting the dog kidney into segments of 2-4mm, and then placing the segments in the stainless steel barrel;
s1-2, dipping: pouring yellow wine 1.4 times the weight of dog kidney into a stainless steel barrel to soak the dog kidney in the yellow wine, sealing and soaking for 90h, and turning over once every 14 h;
s1-3, disinfection: loading the penis et testis Canitis soaked with yellow wine into a clean low-density polyethylene plastic bag, feeding into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol for cleaning and disinfection;
s1-4, drying: transferring the sterilized dog kidney into a clean vacuum drying chamber, placing the dog kidney in a vacuum drying oven, controlling the drying temperature at 70 deg.C and the vacuum degree at (-0.02) - (-0.07) MPa, and drying for 20h to obtain dog kidney with water content of 2.02% for use.
Preparation example 4
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating dog kidney, and the cow kidney is soaked in yellow wine with the weight 0.8 times of that of the dog kidney in the preparation example.
Preparation example 5
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating dog kidney, and the cow kidney is soaked in yellow wine with the weight 0.4 times of that of the dog kidney in the preparation example.
Preparation example 6
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating dog kidney, and the cow kidney is soaked in yellow wine with the weight 1.4 times of that of the dog kidney in the preparation example.
Preparation example 7
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating dog kidney, and the cow kidney is soaked in yellow wine with the weight 1.8 times of that of the dog kidney in the preparation example.
Preparation example 8
The difference between the preparation example and the preparation example 1 is that the dog kidney is soaked in yellow wine for 45h in the process of treating the dog kidney, and the dog kidney is soaked in yellow wine for 45h in the preparation example.
Preparation example 9
The difference between the preparation example and the preparation example 1 is that the dog kidney is soaked in yellow wine for 90h in the preparation example, but the dog kidney is soaked in yellow wine for 90h in the treatment process of the dog kidney.
Preparation example 10
The present preparation example differs from preparation example 1 in that the dog kidney was not impregnated with yellow wine in the treatment of the dog kidney.
The treatment process of the dog kidney specifically comprises the following steps:
s1-1, washing: placing the dog kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until no dust or stain is on the surface of the dog kidney, and cutting the dog kidney into segments of 2-4 mm;
s1-2, placing the dog kidney cut into small segments in a clean low-density polyethylene plastic bag, feeding the dog kidney into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol to clean and sterilize;
s1-3, drying, the steps are the same as the preparation example 1, and the dog kidney with the water content of 2.13% is obtained.
Preparation example of donkey Kidney
Preparation example 1
The treatment process of donkey kidney specifically comprises the following steps:
s1-1, washing: placing the collected donkey kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until no dust or stain is on the surface of the donkey kidney, cutting the donkey kidney into sections with the specification of 2-4mm, and placing the donkey kidney cut into small sections in the stainless steel barrel;
s1-2, dipping: pouring yellow wine 1 time of donkey kidney weight into a stainless steel barrel to soak donkey kidney in the yellow wine, then sealing and soaking for 72h, and turning over once every 12 h;
s1-3, disinfection: loading donkey kidney soaked with yellow wine into a clean low-density polyethylene plastic bag, feeding the donkey kidney into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol for cleaning and disinfection;
s1-4, drying: transferring the sterilized donkey kidney into a clean vacuum drying chamber, placing the donkey kidney into a vacuum drying oven, controlling the drying temperature at 65 ℃ and the vacuum degree at (-0.02) - (-0.07) MPa, and drying for 23h to obtain the donkey kidney with the water content of 3.36%.
Preparation example 2
The difference between the preparation example and the preparation example 1 is that the specific technological parameters in the treatment process of the donkey kidney are different, and the treatment process of the donkey kidney specifically comprises the following steps:
s1-1, washing: placing the collected donkey kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until no dust or stain is on the surface of the donkey kidney, cutting the donkey kidney into sections with the specification of 2-4mm, and placing the donkey kidney cut into small sections in the stainless steel barrel;
s1-2, dipping: pouring yellow wine 0.8 times the weight of donkey kidney into a stainless steel barrel to soak donkey kidney in the yellow wine, then sealing and soaking for 45h, and turning over once every 10 h;
s1-3, disinfection: loading donkey kidney soaked with yellow wine into a clean low-density polyethylene plastic bag, feeding the donkey kidney into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol for cleaning and disinfection;
s1-4, drying: transferring the sterilized donkey kidney into a clean vacuum drying chamber, placing the donkey kidney in a vacuum drying oven, controlling the drying temperature at 60 deg.C and the vacuum degree at (-0.02) - (-0.07) MPa, and drying for 24h to obtain donkey kidney with water content of 2.64%.
Preparation example 3
The difference between the preparation example and the preparation example 1 is that the specific technological parameters in the treatment process of the donkey kidney are different, and the treatment process of the donkey kidney specifically comprises the following steps:
s1-1, washing: placing the collected donkey kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until no dust or stain is on the surface of the donkey kidney, cutting the donkey kidney into sections with the specification of 2-4mm, and placing the donkey kidney cut into small sections in the stainless steel barrel;
s1-2, dipping: pouring yellow wine 1.4 times the weight of donkey kidney into a stainless steel barrel to soak donkey kidney in the yellow wine, sealing and soaking for 90h, and turning over once every 14 h;
s1-3, disinfection: loading donkey kidney soaked with yellow wine into a clean low-density polyethylene plastic bag, feeding the donkey kidney into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol for cleaning and disinfection;
s1-4, drying: transferring the sterilized donkey kidney into a clean vacuum drying chamber, placing the donkey kidney in a vacuum drying oven, controlling the drying temperature at 70 deg.C and the vacuum degree at (-0.02) - (-0.07) MPa, and drying for 22h to obtain donkey kidney with water content of 3.53%.
Preparation example 4
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating donkey kidney, and the donkey kidney is soaked in the yellow wine with the weight of 0.8 time of that of the donkey kidney in the preparation example.
Preparation example 5
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating donkey kidney, and the donkey kidney is soaked in the yellow wine with the weight 0.5 times of the weight of the donkey kidney in the preparation example.
Preparation example 6
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating donkey kidney, and the donkey kidney is soaked by yellow wine with the weight 1.4 times of that of the donkey kidney in the preparation example.
Preparation example 7
The difference between the preparation example and the preparation example 1 is that the dosage of yellow wine is different in the process of treating donkey kidney, and the donkey kidney is soaked in the yellow wine with the weight 1.8 times of that of the donkey kidney in the preparation example.
Preparation example 8
The difference between the preparation example and the preparation example 1 is that the donkey kidney is soaked in yellow wine for 45h in the preparation example, but the time for soaking the donkey kidney in the yellow wine is different in the donkey kidney treatment process.
Preparation example 9
The difference between the preparation example and the preparation example 1 is that the donkey kidney is soaked in yellow wine for 90h in the preparation example, but the time for soaking the donkey kidney in the yellow wine is different in the donkey kidney treatment process.
Preparation example 10
The present preparation example is different from preparation example 1 in that donkey kidney is not impregnated with yellow wine in the process of treating donkey kidney.
The treatment process of donkey kidney specifically comprises the following steps:
s1-1, washing: placing the collected donkey kidney in a stainless steel barrel, injecting drinking water, repeatedly cleaning until no dust or stain is on the surface of the donkey kidney, and cutting the donkey kidney into sections with the specification of 2-4 mm;
s1-2, placing the donkey kidney cut into small segments in a clean low-density polyethylene plastic bag, feeding the donkey kidney into a clean area, and wiping the outside of the low-density polyethylene plastic bag with 75% ethanol to clean and disinfect the donkey kidney;
s1-3, drying, the steps are the same as the preparation example 1, and the donkey kidney with the water content of 2.01 percent is obtained.
Example 1
The embodiment provides a preparation method of a ginseng and pilose antler three-kidney composition, which comprises the following steps:
s1, taking 60Kg of prepared beef kidney, 90Kg of donkey kidney, 9Kg of dog kidney, 30Kg of sun-dried ginseng and 15Kg of hairy antler for later use;
s2, crushing the dried ox kidney, donkey kidney and dog kidney, the sun-dried ginseng and the hairy antler, sieving by a 100-mesh sieve, and reserving sieved substances to obtain mixed powder;
s3, mixing the mixed powder in a mixer for 20min, and then sieving the mixed powder by a 100-mesh sieve to obtain the ginseng antler and three-kidney composition.
The ginseng-pilose antler-three-kidney composition is obtained by the preparation method.
Wherein, the bovine kidney was prepared according to preparation example 1 of bovine kidney preparation example, the donkey kidney was prepared according to preparation example 1 of donkey kidney preparation example, and the dog kidney was prepared according to preparation example 1 of dog kidney preparation example.
Example 2
The difference between this example and example 1 is that, the process parameters of the preparation method of the ginseng-pilose antler-three-kidney composition provided in this example are different, and the selected bovine kidney, donkey kidney and dog kidney are prepared by different preparation examples, and the others are the same as example 1.
A preparation method of a ginseng-pilose antler-three-kidney composition comprises the following steps:
s1, taking 45Kg of prepared beef kidney, 75Kg of donkey kidney, 5Kg of prepared dog kidney, 20Kg of sun-dried ginseng and 10Kg of prepared yellow hairy antler for later use;
s2, crushing the dried ox kidney, donkey kidney and dog kidney, the sun-dried ginseng and the hairy antler, sieving by a 100-mesh sieve, and reserving sieved substances to obtain mixed powder;
s3, mixing the mixed powder in a mixer for 15min, and then sieving the mixed powder by a 100-mesh sieve to obtain the ginseng antler and three-kidney composition.
Wherein the bovine kidney was prepared according to preparation example 2 of bovine kidney preparation example, the donkey kidney was prepared according to preparation example 2 of donkey kidney preparation example, and the dog kidney was prepared according to preparation example 2 of dog kidney preparation example.
Example 3
The difference between this example and example 1 is that, the process parameters of the preparation method of the ginseng-pilose antler-three-kidney composition provided in this example are different, and the selected bovine kidney, donkey kidney and dog kidney are prepared by different preparation examples, and the others are the same as example 1.
A preparation method of a ginseng-pilose antler-three-kidney composition comprises the following steps:
s1, taking 75Kg of prepared beef kidney, 105Kg of donkey kidney, 13Kg of prepared dog kidney, 40Kg of sun-dried ginseng and 20Kg of prepared yellow hairy antler for later use;
s2, crushing the dried ox kidney, donkey kidney and dog kidney, the sun-dried ginseng and the hairy antler, sieving by a 100-mesh sieve, and reserving sieved substances to obtain mixed powder;
s3, placing the mixed powder into a mixer to mix for 25min, and then sieving the mixed powder through a 100-mesh sieve to obtain the ginseng antler and kidney composition.
Wherein the bovine kidney was prepared according to preparation example 3 of bovine kidney preparation example, the donkey kidney was prepared according to preparation example 3 of donkey kidney preparation example, and the dog kidney was prepared according to preparation example 3 of dog kidney preparation example.
Example 4
This example differs from example 1 in that the bovine kidney, donkey kidney and dog kidney selected in this example were prepared by different preparations. Wherein bovine kidney was prepared according to preparation example 4 of bovine kidney preparation example, donkey kidney was prepared according to preparation example 4 of donkey kidney preparation example, and dog kidney was prepared according to preparation example 4 of dog kidney preparation example, and the other examples are the same as example 1.
Example 5
This example differs from example 1 in that the bovine kidney, donkey kidney and dog kidney selected in this example were prepared by different preparations. Wherein bovine kidney was prepared according to preparation example 6 of bovine kidney preparation example, donkey kidney was prepared according to preparation example 6 of donkey kidney preparation example, and dog kidney was prepared according to preparation example 6 of dog kidney preparation example, and the other examples were the same as example 1.
Example 6
This example differs from example 1 in that the bovine kidney, donkey kidney and dog kidney selected in this example were prepared by different preparations. Wherein bovine kidney was prepared according to preparation example 8 of bovine kidney preparation example, donkey kidney was prepared according to preparation example 8 of donkey kidney preparation example, and dog kidney was prepared according to preparation example 8 of dog kidney preparation example, and the other examples are the same as example 1.
Example 7
This example differs from example 1 in that the bovine kidney, donkey kidney and dog kidney selected in this example were prepared by different preparations. Wherein bovine kidney was prepared according to preparation example 9 of bovine kidney preparation example, donkey kidney was prepared according to preparation example 9 of donkey kidney preparation example, and dog kidney was prepared according to preparation example 9 of dog kidney preparation example, and the other examples were the same as example 1.
Example 8
This example differs from example 1 in that the bovine kidney of this example was prepared according to preparation example 4 of the bovine kidney preparation example, and the other examples are the same as example 1.
Example 9
This example is different from example 1 in that the bovine kidney of this example was prepared by preparation example 6 of bovine kidney preparation example, and the other examples were the same as example 1.
Example 10
This example is different from example 1 in that the donkey kidney of this example is prepared by preparation example 4 of donkey kidney preparation example, and the other steps are the same as example 1.
Example 11
This example is different from example 1 in that the donkey kidney of this example is prepared by preparation example 6 of donkey kidney preparation example, and the other steps are the same as example 1.
Comparative example 1
This comparative example is different from example 1 in that the bovine kidney, the donkey kidney and the dog kidney selected in this example were prepared by different preparation examples. Wherein bovine kidney was prepared according to preparation example 5 of bovine kidney preparation example, donkey kidney was prepared according to preparation example 5 of donkey kidney preparation example, and dog kidney was prepared according to preparation example 5 of dog kidney preparation example, and the other examples are the same as example 1.
Comparative example 2
The present comparative example is different from example 1 in that the bovine kidney, the donkey kidney and the dog kidney selected in the present comparative example are prepared by different preparation examples. Wherein bovine kidney was prepared according to preparation example 7 of bovine kidney preparation example, donkey kidney was prepared according to preparation example 7 of donkey kidney preparation example, and dog kidney was prepared according to preparation example 7 of dog kidney preparation example, and the other examples were the same as example 1.
Comparative example 3
The comparative example is different from example 1 in that the bovine kidney of the comparative example is prepared by preparation example 5 of bovine kidney preparation example, and the other steps are the same as example 1.
Comparative example 4
This comparative example differs from example 1 in that the bovine kidney of this comparative example was prepared by preparation example 7 of the bovine kidney preparation example, and the other examples are the same as example 1.
Comparative example 5
The present comparative example is different from example 1 in that the donkey kidney of the present comparative example is prepared by preparation example 5 of donkey kidney preparation example, and the others are the same as example 1.
Comparative example 6
The present comparative example is different from example 1 in that the donkey kidney of the present comparative example is prepared by preparation example 7 of donkey kidney preparation example, and the others are the same as example 1.
Comparative example 1
This comparative example is different from example 1 in that the bovine kidney, the donkey kidney and the dog kidney selected in this comparative example were prepared by different preparation examples. Wherein bovine kidney was prepared according to preparation example 10 of bovine kidney preparation example, donkey kidney was prepared according to preparation example 10 of donkey kidney preparation example, and dog kidney was prepared according to preparation example 10 of dog kidney preparation example, and the other examples were the same as example 1.
Comparative example 2
The difference between the comparative example and the example 1 is that the raw materials for preparing the ginseng-pilose antler-three-kidney composition of the comparative example are not added with dog kidney, and the other steps are the same as the example 1.
Comparative example 3
The comparative example is different from example 1 in that the mixing time of the mixed powder is 10min when the step S3 is performed in preparing the ginseng and pilose antler triple kidney composition.
The ginseng antler three-kidney composition obtained in the embodiment is placed in an oral medicinal high-density polyethylene bottle and sealed to obtain a ginseng antler three-kidney powder product. The medicine loading amount of each bottle is 3.00-3.08 g. Then packaging and storing at 18-26 deg.C.
The ginseng and pilose antler triple kidney compositions prepared in the above examples 1 to 10, comparative examples 1 to 6 and comparative examples 1 to 3 were subjected to the related performance tests, as follows.
The toxicity test method comprises the following steps: 20 adult white mice weighing 18-22g were selected for testing and randomly divided into two groups of 10 mice each, a blank group and a test group. The ginseng antler three-kidney composition prepared in the example 1 is fed to a test group, 20g of daily dosage is soaked in water and evenly mixed, then, the stomach of 10 white rats of the test group is irrigated, and the observation is carried out for 7 days; the blank group of white mice was left untreated.
After 7 days, 10 mice were kept healthy, and no abnormality was observed in activity, diet, hair, and excretion, and no toxic reaction was observed. Toxicity tests prove that the ginseng-pilose antler-three-kidney composition has no toxic effect and adverse reaction, and is safe and reliable in clinical application.
Animal experiments: in the animal test performed in the present application, the test animal is a Kunming mouse.
Second, kidney-tonifying and yang-strengthening experiment for kidney deficiency (castration) mice
176 male mice leaving one month from milk and weighing 9-15g were randomly divided into 21 groups of 8 mice each, namely, a normal control group (administered with physiological saline, 10mL/kg) and a model control group (administered with physiological saline, 10mL/kg), wherein the ginseng antler three-kidney composition medicament experimental group (19 groups in total, the ginseng antler three-kidney composition prepared in examples 1-11, comparative examples 1-6 and comparative examples 1-3 was administered with 30g/kg/d, which is 10 times of 3g/kg of human clinical daily dose).
Except for the normal control group, rats of the other groups were anesthetized with pentobarbital sodium (45mg/kg), the scrotal skin was disinfected, and the bilateral testes were removed. The intramuscular injection of penicillin was 2 ten thousand U/kg/d for 3 days after the operation. On the third day after operation, the group starts to give the ginseng-pilose antler-three-kidney composition or the normal saline for 14 days continuously. After 24h of the last administration, the animals were sacrificed and the seminal vesicles-prostate and glandulae preputiales were harvested and weighed and their average weight calculated, the specific results are shown in table 1.
TABLE 1 Effect of Ginseng and pilose antler three-kidney composition on seminal vesicle-prostate and glandulae preputiales of castrated mice
| Detection group | Seminal fluid vesicle-prostate (mg/10g) | Glandula preputipes (mg/10g) |
| Normal control group | 15.26 | 8.51 |
| Model control group | 4.33 | 4.32 |
| Example 1 | 13.34 | 8.17 |
| Example 2 | 11.58 | 7.89 |
| Example 3 | 11.83 | 7.88 |
| Example 4 | 12.38 | 7.98 |
| Example 5 | 12.56 | 8.06 |
| Example 6 | 12.97 | 8.02 |
| Example 7 | 12.34 | 7.97 |
| Example 8 | 12.41 | 8.03 |
| Example 9 | 12.82 | 7.99 |
| Example 10 | 12.88 | 8.11 |
| Example 11 | 12.73 | 8.06 |
| Comparative example 1 | 11.02 | 7.03 |
| Comparative example 2 | 11.33 | 7.52 |
| Comparative example 3 | 11.07 | 7.21 |
| Comparative example 4 | 10.93 | 7.67 |
| Comparative example 5 | 11.36 | 7.64 |
| Comparative example 6 | 11.43 | 7.72 |
| Comparative example 1 | 10.26 | 6.63 |
| Comparative example 2 | 10.95 | 6.56 |
| Comparative example 3 | 11.06 | 7.61 |
As can be seen from the results of comparing the data of comparative example 1 and example 1 (or examples 2 to 11, comparative examples 1 to 6) in table 1, the effects of strengthening yang and tonifying kidney of the prepared ginseng and pilose antler three-kidney composition can be effectively enhanced by treating the three kidneys (bovine kidney, dog kidney and donkey kidney) with yellow wine. The mass of the seminal vesicle-prostate gland of the experimental mice in examples 1-11 was maintained at 11.58-13.34mg/10g, much higher than that of the experimental mice of comparative example 1 (10.26mg/10 g); the mass of the preputial gland of the experimental mice in examples 1 to 11 was maintained at 7.97 to 8.17mg/10g, which was also higher than that of the experimental mouse in comparative example 1 (6.63mg/10 g).
In the process of triple-kidney treatment, the data results of comparative example 1, example 4-7 and comparative example 1-2 show that the time and amount of yellow wine soaking have great influence on the yang-strengthening and kidney-tonifying effects of the finally prepared ginseng and pilose antler triple-kidney composition: when the amount of the yellow wine is 0.8-1.4 times of the weight of the three kidneys (example 4 and example 5), the prepared ginseng-pilose antler three-kidney composition has good effects of tonifying yang and tonifying kidney. However, it is seen from the data of comparative examples 8 to 11 and comparative examples 3 to 6 that the change in the amount of yellow wine during the treatment of bovine kidney has a greater effect on the kidney-tonifying and yang-strengthening effects of the finally prepared ginseng antler triple-kidney composition than the change in the amount of yellow wine during the treatment of donkey kidney: when the amount of yellow wine for treating the bovine kidney is less than 0.8 times of the weight of the bovine kidney (comparative example 3, the amount of yellow wine for treating the bovine kidney is 0.5 times of the weight of the bovine kidney), the mass of the seminal vesicle-prostate of the experimental mouse is reduced from 13.34mg/10g (example 1) to 11.07mg/10g (comparative example 3), and is reduced by 17.02%; when the amount of yellow wine for treating donkey kidney is less than 0.8 times of the mass of donkey kidney (comparative example 5, the amount of yellow wine for treating donkey kidney is 0.5 times of the mass of donkey kidney), the mass of seminal vesicle-prostate of experimental mouse is reduced from 13.34mg/10g (example 1) to 11.36mg/10g (comparative example 3), which is reduced by 14.84%.
(III) Effect on mouse appendage weight and sperm count
70 mice weighing 20-25g were randomly divided into 7 groups of 10 mice each, 1 normal control group and 6 experimental groups. Normal control group is administered with normal saline at a dose of 10mL/kg once a day for 14 days; the ginseng-pilose antler three-kidney composition prepared in example 1, example 4-5 and comparative example 1-3 was administered to 6 experimental groups at a dose of 30g/kg, once a day for 14 days. Then, 30min after the last administration, the mice were sacrificed by cervical dislocation and were weighed after taking out bilateral epididymis and testis. Then immediately cutting epididymis into pieces, placing into a test tube containing 0.2mL of physiological saline, placing in a 34 deg.C water bath for 10min, taking out 0.1mL, adding 5% NaHCO3After the solution was diluted, sperm counts were counted on a counting plate. The specific results are shown in Table 2.
TABLE 2 Effect of Ginseng and Rong Sanshen composition on the number of mouse appendage and sperm
The data results in table 2 show that the ginseng-pilose antler three-kidney composition has a good yang-strengthening effect, can effectively increase the number of sperms of a mouse, and ensures a certain sexual function.
Influence of ginseng and pilose antler three-kidney composition on fatigue resistance of mice
70 mice weighing 20-25g were randomly divided into 7 groups of 10 mice each, 1 normal control group and 6 experimental groups. The ginseng-pilose antler triple-kidney compositions prepared in examples 1, 4 to 5 and comparative examples 1 to 3 were administered to 6 experimental groups at a dose of 30g/kg, respectively, and the normal control group was administered with the same dose (30g/kg) of physiological saline once a day for 7 days. Injecting clear water into the swimming tank after the last administration for 1h, adjusting and keeping the water temperature at 25 ℃; each mouse was placed under a load of 10% of the body mass in the mouse's tail and forced to swim, and the time from the time the animal was submerged until the head was completely submerged and did not float out of the water for 10 seconds was measured using a stopwatch. The specific results are shown in Table 3.
TABLE 3 Effect of Ginseng and pilose antler three kidney composition on swimming time of mice
| Detection group | Swimming time (min) |
| Normal control group | 3.14 |
| Example 1 | 5.28 |
| Example 4 | 5.12 |
| Example 5 | 5.16 |
| Comparative example 1 | 4.52 |
| Comparative example 2 | 4.87 |
| Comparative example 3 | 5.01 |
From the data results of table 3, it can be seen that the anti-fatigue time of the mice administered with the ginseng and pilose antler triple kidney composition of the present application was increased, and the swimming time of the mice was increased from 3.14min to 5.28min, which was increased by 68.15%, by comparing the data of example 1 and examples 4-5 with the swimming time of the mice of the normal control group. By comparing the data results of the comparative example 1 and the example 1, the prepared ginseng antler three-kidney composition can effectively improve the anti-fatigue effect of mice after the three kidneys are soaked in yellow wine: compared with the comparative example 1, the swimming time of the mice to which the ginseng antler and three-kidney composition prepared in the example 1 is administered is prolonged from 4.52min to 5.28min, which is 16.8% longer.
And (V) treating male patients by using the ginseng and pilose antler three-kidney composition prepared in the example 1. The specific process is as follows: selecting 60 patients, wherein 3 patients of 20-30 years old are mild patients; 7 patients between 30 and 40 years of age, 6 of which were mild patients and 1 of which was moderate; 12 patients between 40-50 years of age, 6 mild patients, 4 moderate patients, 2 severe patients; 28 patients between 50 and 60 years of age, 4 of which were mild, 10 of which were moderate, and 14 of which were severe. The ginseng-pilose antler-three-kidney composition is prepared into powder, and is orally taken according to the dosage of 1.5 g/time, wherein one time is taken every day, and one week is a treatment course.
After one treatment course, 3 mild patients of 20-30 years old all cured, and 6 mild patients and 1 moderate patient of 30-40 years old all cured. 6 mild patients between 40-50 years of age and 3 moderate patients among them were cured; while 1 moderate patient turned to mild, 2 severe patients turned to moderate. 4 mild patients between 50 and 60 years of age and 4 moderate patients among them were cured; while the remaining 6 moderate patients were mild, 10 of 14 severe patients were moderate, and 4 of them were severe although the symptoms were improved. That is, after the first treatment course, the ginseng-pilose antler-three-kidney composition (powder) of example 1 of the present application has a cure rate of 54% for patients with kidney deficiency.
The treatment was continued on the patients who did not heal, and after the second course, 1 mild patient and 2 moderate patients aged 40-50 after the first course were healed. After the first course of treatment 6 mild patients and 10 moderate patients aged 50-60 were cured, 4 severe patients were converted to moderate patients. That is, after the second treatment course, the ginseng-pilose antler-three-kidney composition (powder) of example 1 of the present application has a cure rate of 92% for patients with kidney deficiency.
The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.
Claims (10)
1. The preparation method of the ginseng-pilose antler-three-kidney composition is characterized by comprising the following steps of:
s1, respectively soaking the segmented ox kidney, donkey kidney and dog kidney in yellow wine, sterilizing and drying for later use;
s2, mixing and crushing the dried ox kidney, donkey kidney and dog kidney, sun-dried ginseng and hairy antler, and sieving by a 100-mesh sieve to obtain undersize products, namely mixed powder;
s3, mixing the mixed powder for 15-25min, and then sieving with a 100-mesh sieve to obtain the ginseng antler and three-kidney composition.
2. The method for preparing a Shenrongsanshen composition as claimed in claim 1, wherein the process of treating the bovine kidney comprises the following steps:
s1-1, cleaning the beef kidney and cutting into segments;
s1-2, soaking the beef kidney in yellow wine in a sealed manner for 45-90 hours, and turning over every 10-14 hours, wherein the addition amount of the yellow wine is 0.8-1.4 times of the weight of the beef kidney;
s1-3, disinfecting the beef kidney soaked with the yellow wine;
s1-4, drying the disinfected beef kidney in vacuum until the water content is lower than 4%.
3. The method for preparing Shenrongsanshen composition as claimed in claim 1, wherein the processing procedure of the donkey kidney comprises the following steps:
s2-1, cleaning donkey kidney and cutting into segments;
s2-2, soaking donkey kidney in yellow wine in a sealed manner for 45-90h, and turning over every 10-14h, wherein the addition amount of the yellow wine is 0.8-1.4 times of the weight of the donkey kidney;
s2-3, disinfecting the donkey kidney soaked with yellow wine;
s2-4, drying the sterilized donkey kidney in vacuum until the water content is lower than 4%.
4. The method for preparing Shenrongsanshen composition as claimed in claim 1, wherein the process of treating the dog kidney comprises the following steps:
s3-1, cleaning dog kidney, and cutting into segments;
s3-2, soaking the dog kidney in yellow wine, sealing and soaking for 45-90h, and turning over once every 10-14h, wherein the addition amount of the yellow wine is 0.8-1.4 times of the weight of the dog kidney;
s3-3, disinfecting the dog kidney soaked with yellow wine;
s3-4, drying the disinfected dog kidney in vacuum until the water content is lower than 4%.
5. The preparation method of the ginseng and pilose antler triple-kidney composition according to claim 1, wherein the ginseng and pilose antler triple-kidney powder is prepared from the following raw materials, by weight, 20-40 parts of sun-dried ginseng, 10-20 parts of yellow hair pilose antler, 45-75 parts of beef kidney, 75-105 parts of donkey kidney, and 5-13 parts of dog kidney.
6. The method for preparing SHENRONGTRISHEN composition as claimed in any of claims 2-4, wherein the vacuum drying is performed under (-0.02) - (-0.07) MPa for 20-24h at 60-70 deg.C.
7. A ginseng and pilose antler triple kidney composition, characterized by being prepared by the method of any one of claims 1 to 5.
8. The Shenrongsanshen composition as claimed in claim 7, wherein conventional adjuvants are added into the composition to prepare clinically acceptable one of pills, powders, tablets, granules, capsules, microcapsules, ointments, medicated wine, syrups and oral liquids by conventional processes.
9. The Shenrongsanshen composition as claimed in claim 8, wherein the composition is prepared as powder or capsule.
10. The Shenrongsanshen composition as claimed in claim 8, wherein when said composition is prepared into powder, said mixed composition is put into a high density polyethylene bottle for oral administration and sealed.
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| CN1368231A (en) * | 2001-02-01 | 2002-09-11 | 杨孟君 | Nano medicine 'Shenrongsanshen' and its preparing process |
| CN109999124A (en) * | 2019-05-09 | 2019-07-12 | 参之道(通化)生物科技有限公司 | A kind of Black people join deer whip slice prescription and preparation method thereof |
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|---|---|---|---|---|
| CN1368231A (en) * | 2001-02-01 | 2002-09-11 | 杨孟君 | Nano medicine 'Shenrongsanshen' and its preparing process |
| CN109999124A (en) * | 2019-05-09 | 2019-07-12 | 参之道(通化)生物科技有限公司 | A kind of Black people join deer whip slice prescription and preparation method thereof |
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| 彭成: "《中国临床药物大辞典，中药成方制剂卷：全2卷》", 31 August 2018, 中国医药科技出版社 * |
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Application publication date: 20211126 |