CN113520895B - Environment-friendly no-clean disinfection hand sanitizer and preparation method thereof - Google Patents
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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Abstract
The invention discloses an environment-friendly no-clean disinfection hand sanitizer and a preparation method thereof, wherein the environment-friendly no-clean disinfection hand sanitizer comprises the following components in parts by mass: 0.4-0.6 part of green high-efficiency peptide S3 mother solution, 8-10 parts of glycerol and 90 parts of distilled water, wherein the sequence of the green high-efficiency peptide S3 is VNWSFGCVRVGNRYVCNSRRH-NH 2 . The hand sanitizer has strong cleaning capability, quick volatility and moisturizing function; the performance is mild, and the irritation is small; the hand sanitizer has the advantages that the hand sanitizer is simple in specific components, can continuously inhibit bacteria and has a nursing effect on hands, meanwhile, can achieve the effects of environmental protection and no washing, saves water resources, does not pollute the environment, is green and environment-friendly, is processed and manufactured into the hand sanitizer spray, has the characteristics of being convenient to carry and use, can clean and disinfect skin of a user everywhere, avoids cross infection, and has broad-spectrum and efficient antibacterial effects.
Description
Technical Field
The invention relates to the field of disinfection products, in particular to an environment-friendly no-clean disinfection hand sanitizer and a preparation method thereof.
Background
With the development of the integration and trade globalization of the world economy, cultural background, technical level and environmental protection regulations are becoming three major drivers of globalization development of disinfection washing products, the global disinfection washing products will tend to be diversified, but the products which are energy-saving, water-saving, efficient, mild, convenient to use and meet the requirements of safety and environmental protection will be the main stream direction of the development of the current and future disinfection washing products.
At present, hand cleaning products such as perfumed soap, soap and the like are mainly used for cleaning hands. The soap and the soap have strong cleaning power, but the soap has strong degreasing power, and the skin is strong in tightness after washing, so that the horny layer is thinned after long-term use, and the soap has great irritation and damage to the skin. Most of the conventional common disinfection hand sanitizers have the characteristic of low cleaning strength, and a large amount of foam can be generated in use, so that a large amount of water is required to be consumed, and the disinfection hand sanitizers are difficult to clean and difficult to degrade. And most of the existing disinfectant hand sanitizers are composed of chemical substances, and the chemical substances can irritate the skin of hands and are not suitable for people with skin allergy. In addition, excessive use of disinfectant for a long period of time can lead to increased resistance of environmental bacteria and influence on human health. On the other hand, when the user goes out, the user cannot carry the hand sanitizer or the soap with the user, and in many cases, the user cannot thoroughly clean the hand by using the clean water, and in public places, the user can share one soap or one bottle of the hand sanitizer, so that cross infection can be caused.
The invention patent application with publication number of CN110478293A discloses an environment-friendly no-clean disinfection hand sanitizer, which comprises the following components in percentage by mass: glycerin 1-5%, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer 0.1-1%, triethanolamine 0.1-1%, ethanol 50-70%, fructus Zanthoxyli extract 0.2-0.8%, fructus forsythiae extract 0.1-1%, leuconostoc mesenteroides/radix Raphani fermentation product filtrate 0.2-4%, and water in balance. Although the invention has certain cleaning and sterilizing effects, the invention has complex components, weaker cleaning power and complex preparation method.
The present invention has been made in view of this.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides an environment-friendly no-clean disinfection hand sanitizer, which comprises the following components in parts by mass: 0.4-0.6 part of green high-efficiency peptide S3 mother solution, 8-10 parts of glycerol and 90 parts of distilled water, wherein the sequence of the green high-efficiency peptide S3 is VNWSFGCVRVGNRYVCNSRRH-NH 2 。
Preferably, the composition comprises the following components in parts by weight: 0.5 part of green high-efficiency peptide, 10 parts of glycerol and 89.5 parts of distilled water.
Preferably, in any of the above embodiments, the green highly potent peptide S3 is obtained by Fmoc solid phase synthesis,
the specific process is as follows: the preparation method comprises the steps of performing in the direction from the C end to the N end, firstly activating and coupling a first amino acid of the C end, namely Fmoc-Asn-OH, to CTC resin, and then deprotecting a protective group Fmoc to obtain X-Asn+CTC resin; activating and coupling the second amino acid at the C end, namely Fmoc-Ser-OH; the amino acid is sequentially accessed from the C end to the last amino acid according to the procedure; adding acetic anhydride, washing 3 times with DMF, washing with methanol, and drying overnight in a nitrogen dryer; adding the dried resin into TFA cutting fluid containing TIPS for cutting; filtering, adding glacial ethyl ether into the filtrate to precipitate S3, and placing the precipitate into a rotary evaporator for rotary evaporation to obtain S3; after dissolving S3 in water, it is passed through a high performance liquid C18 column with mobile phase a:0.1% tfa+water; mobile phase B:0.075% TFA+acetonitrile, separating, purifying, freeze drying, and MS identification and analysis to obtain green high-efficiency peptide with C-terminal acetylation and purity of more than 95%.
Preferably, in any of the above schemes, the green high-efficiency peptide S3 mother solution is prepared from distilled water, and the concentration of the green high-efficiency peptide S3 mother solution is 2-5mg/ml.
In any of the above embodiments, it is preferable that the green highly effective peptide S3 solution is prepared from distilled water, and the concentration thereof is 2-4mg/ml.
In any of the above embodiments, it is preferable that the green highly effective peptide S3 solution is prepared from distilled water, and the concentration thereof is 2-3mg/ml.
In any of the above embodiments, the green highly effective peptide S3 solution is preferably prepared from distilled water at a concentration of 2.8mg/ml.
In any of the above schemes, preferably, the environment-friendly no-clean disinfection hand sanitizer is prepared by shaking and uniformly mixing 0.4-0.6 part of green high-efficiency peptide S3 liquid, 8-10 parts of glycerol and 90 parts of distilled water.
In any of the above schemes, preferably, the environment-friendly disposable disinfection hand sanitizer is used in a spray mode.
The invention also provides a preparation method of the environment-friendly washing-free disinfection hand sanitizer, which comprises the specific steps of adopting Fmoc solid-phase synthesis to obtain the green efficient peptide, adding distilled water to obtain a mother solution with a certain concentration, stirring and premixing a mixture of glycerol and distilled water, then vibrating and uniformly mixing with the green efficient peptide S3 solution, and bottling.
Preferably, the green high-efficiency peptide S3 is added into distilled water, the mass percentage concentration of the green high-efficiency peptide is 0.28%, and the green high-efficiency peptide S3 mother liquor is prepared by shaking and uniformly mixing the green high-efficiency peptide S3 for 10 min at 200 rpm under the condition of 4 ℃ in a shaking table.
In any of the above schemes, preferably, 10 mL environmental protection no-clean hand-washing liquid is taken and packaged into spray bottles.
The beneficial effects are that: (1) The environment-friendly efficient peptide hand sanitizer provided by the invention has the advantages of strong cleaning capability, high volatility and moisturizing function.
(2) Has mild performance and small irritation.
(3) The hand sanitizer has the advantages that the hand sanitizer is simple in specific components, can continuously inhibit bacteria and has a nursing effect on hands, meanwhile, the environment-friendly and wash-free effect can be achieved, water resources are saved, the hand sanitizer spray manufactured by processing the hand sanitizer has the characteristics of being convenient to carry and use, a user can clean and disinfect skin everywhere, cross infection is avoided, and the hand sanitizer has a broad-spectrum and efficient antibacterial effect.
Drawings
Fig. 1 is a flow chart of the preparation of the environmentally friendly leave-on sanitizing hand sanitizer according to the preferred embodiment of the present invention.
Detailed Description
The principles and spirit of the present invention will be described below with reference to several exemplary embodiments shown in the drawings. It should be understood that these embodiments are merely described to enable those skilled in the art to better understand and to practice the invention and are not intended to limit the scope of the invention in any way.
Example 1
The environment-friendly no-clean disinfection hand sanitizer in the embodiment consists of 0.5 part of color efficient peptide mother liquor, 10 parts of glycerol and 89.5 parts of distilled water.
The green high-efficiency peptide is obtained through Fmoc solid phase synthesis, and the green high-efficiency peptide S3 is obtained through Fmoc solid phase synthesis, and the specific process is as follows: the preparation method comprises the steps of performing in the direction from the C end to the N end, firstly activating and coupling a first amino acid of the C end, namely Fmoc-Asn-OH, to CTC resin, and then deprotecting a protective group Fmoc to obtain X-Asn+CTC resin; activating and coupling the second amino acid at the C end, namely Fmoc-Ser-OH; the amino acid is sequentially accessed from the C end to the last amino acid according to the procedure; adding acetic anhydride, washing 3 times with DMF, washing with methanol, and drying overnight in a nitrogen dryer; adding the dried resin into TFA cutting fluid containing TIPS for cutting; filtering, adding glacial ethyl ether into the filtrate to precipitate S2, and placing the precipitate into a rotary evaporator for rotary evaporation to obtain S3; after dissolving S3 in water, it is passed through a high performance liquid C18 column with mobile phase a:0.1% tfa+water; mobile phase B:0.075% TFA+acetonitrile, separating, purifying, freeze drying, and MS identification and analysis to obtain green high-efficiency peptide with C-terminal acetylation and purity of more than 95%.
Adding polypeptide S3 of 2.8. 2.8mg into distilled water of 1 mL, shaking, and mixing to obtain mother liquor with concentration of 2.8. 2.8mg/mL.
The components are configured according to the parts by weight, and are evenly mixed by vibration, 10 mL environment-friendly hand-washing liquid without washing is taken and packaged into spray bottles, and the product is prepared.
Example 2
The environment-friendly no-clean disinfection hand sanitizer in the embodiment consists of 0.6 part of color efficient peptide mother liquor, 10 parts of glycerol and 89.4 parts of distilled water.
The green high-efficiency peptide is obtained by Fmoc solid-phase synthesis method, and the purity of the C-terminal acetylation is 95%.
Adding polypeptide S3 of 3mg into distilled water of 1 mL, shaking, and mixing to obtain mother solution with concentration of 3mg/mL.
The components are configured according to the parts by weight, and are evenly mixed by vibration, 10 mL environment-friendly hand-washing liquid without washing is taken and packaged into spray bottles, and the product is prepared.
Example 3
The environment-friendly no-clean disinfection hand sanitizer in the embodiment consists of 0.4 part of color efficient peptide mother liquor, 8 parts of glycerol and 81.6 parts of distilled water.
The green high-efficiency peptide is obtained by Fmoc solid-phase synthesis method, and the purity of the C-terminal acetylation is 96%.
Adding polypeptide S3 of 4mg into distilled water of 1 mL, shaking, and mixing to obtain mother solution with concentration of 4mg/mL.
The components are configured according to the parts by weight, and are evenly mixed by vibration, 10 mL environment-friendly hand-washing liquid without washing is taken and packaged into spray bottles, and the product is prepared.
Example 4
The environmental protection no-clean disinfection hand sanitizer prepared in example 1 is subjected to antibacterial effect detection.
The detection steps are as follows: a) The sterilized cotton swab was used to wet in sterile saline and applied back and forth ten times to the left finger curved surface of 20 volunteers. Then subtracting the handheld part of the cotton swab, soaking the rest part (namely the lower part) of the cotton swab in a centrifuge tube containing 2 mL sterile normal saline, and vortex oscillating 40 s to serve as sample liquid; b) Spraying a spray bottle containing the environment-friendly no-clean hand sanitizer, a commercial commodity control 1 (containing 75% alcohol) and a commercial commodity control 2 (containing 60% alcohol) on the fingers of a volunteer at a short distance (3-10 cm) for one time respectively, uniformly coating the spray bottle with a sterilized sterile cotton swab, and repeating the step a) after the solution is naturally dried; c) 50 μl of each sample was applied to MHA plates, each plate was incubated at 37deg.C in reverse for 24 h and 48 h, and twice colony counts were performed to calculate the antibacterial rate.
Finally, the detection results of the antibacterial effect of the environment-friendly no-wash hand sanitizer, the commercial commodity control 1 (containing 75% alcohol) and the commercial commodity control 2 (containing 60% alcohol) on the finger of the subject after one injection are shown in the following table, and the antibacterial effect is judged that the antibacterial rate is more than or equal to 50% -90%:
from the results, the antibacterial rate (24 h) and the antibacterial rate (48 h) of the environment-friendly disposable disinfectant hand sanitizer provided by the invention are obviously superior to those of a control group, and the defect that skin itch, red swelling and the like do not occur after the product is proved.
Example 5
The main components of the product are green high-efficiency peptide, glycerol and distilled water.
Wherein the hemolysis test steps of the polypeptide are as follows:
a) Collecting animal blood in heparin sodium anticoagulation tube, washing red blood cells with 0.9% physiological saline for three times until the supernatant is colorless and transparent, thereby preparing 8% red blood cell suspension;
b) The green high-efficiency peptide is diluted by a double ratio of 0.9% physiological saline to a final concentration of 1-256 mug/mL;
c) Adding the same volume of 8% erythrocyte suspension and a series of S3 diluents into a 96-well plate, placing the mixture into a 37 ℃ constant temperature incubator for standing and incubation for 1 h, centrifuging at 1500 rpm for 5 min, placing the mixture into the 96-well plate, and detecting an ultraviolet absorbance value under 540 and nm by using an enzyme-labeled instrument;
d) 0.1% Triton X-100 and normal saline were used as positive and blank controls, respectively, and three replicates were set for each concentration; e) The calculation formula of the hemolysis degree is as follows:
degree of hemolysis (%) = [ (absssample-absssaline)/(abs0.1% Triton X-100-absssaline) ]x100%.
Finally, the hemolysis rate of the polypeptide S3 and 0.1% Triton X-100 at the concentration of 256 mug/mL is 3.83% and 100%, which shows that the green high-efficiency peptide component of the invention has no or very low hemolysis to animal red blood cells, and the green high-efficiency peptide S3 component is safe and nontoxic.
The environment-friendly no-clean disinfectant hand sanitizer provided by the invention has the characteristics of simple components, continuous bacteria inhibition, nursing effect on hands, easiness in degradation, environment-friendly no-clean effect, water resource saving, convenience in carrying and use when being processed and manufactured into the hand sanitizer spray, and capability of cleaning and disinfecting skin everywhere by a user, cross infection prevention and broad-spectrum and high-efficiency antibacterial effect.
Specific examples are set forth herein to illustrate the invention in detail, and the description of the above examples is only for the purpose of aiding in understanding the core concept of the invention. It should be noted that any obvious modifications, equivalents, or other improvements to those skilled in the art without departing from the inventive concept are intended to be included in the scope of the present invention.
Sequence listing
<110> university of academy of sciences of China
<120> an environmental protection no-clean disinfection hand sanitizer and preparation method thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 21
<212> PRT
<213> Artificial sequence (Artificial Sequence)
<400> 1
Val Asn Trp Ser Phe Gly Cys Val Arg Val Gly Asn Arg Tyr Val Cys
1 5 10 15
Asn Ser Arg Arg His
20
Sequence listing
<110> university of academy of sciences of China
<120> an environmental protection no-clean disinfection hand sanitizer and preparation method thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 21
<212> PRT
<213> Artificial sequence (Artificial Sequence)
<400> 1
Val Asn Trp Ser Phe Gly Cys Val Arg Val Gly Asn Arg Tyr Val Cys
1 5 10 15
Asn Ser Arg Arg His
20
Claims (9)
1. The environment-friendly no-clean disinfection hand sanitizer comprises the following components in parts by mass: 0.4-0.6 part of green high-efficiency peptide S3 mother liquor, 8-10 parts of glycerol and 90 parts of distilled water, wherein the green high-efficiency peptide S3 mother liquor is prepared from distilled water, the concentration is 2-5mg/ml, and the green high-efficiency peptide S3 is the polypeptide with the acetylated sequence C end shown in SEQ ID NO. 1.
2. The environment-friendly no-clean disinfection hand sanitizer of claim 1, comprising the following components in parts by weight: 0.5 part of green high-efficiency peptide S3 mother liquor, 10 parts of glycerol and 89.5 parts of distilled water.
3. The environment-friendly no-clean disinfectant hand sanitizer according to claim 2, wherein the green high-efficiency peptide S3 is obtained by Fmoc solid-phase synthesis, and the specific process is as follows: the preparation method comprises the steps of performing in the direction from the C end to the N end, firstly activating and coupling a first amino acid of the C end, namely Fmoc-Asn-OH, to CTC resin, and then deprotecting a protective group Fmoc to obtain X-Asn+CTC resin; activating and coupling the second amino acid at the C end, namely Fmoc-Ser-OH; the amino acid is sequentially accessed from the C end to the last amino acid according to the procedure; adding acetic anhydride, washing 3 times with DMF, washing with methanol, and drying overnight in a nitrogen dryer; adding the dried resin into TFA cutting fluid containing TIPS for cutting; filtering, adding glacial ethyl ether into the filtrate to precipitate S3, and placing the precipitate into a rotary evaporator for rotary evaporation to obtain S3; after dissolving S3 in water, it is passed through a high performance liquid C18 column with mobile phase a:0.1% tfa+water; mobile phase B:0.075% TFA+acetonitrile, separating, purifying, freeze drying, and MS identification and analysis to obtain green high-efficiency peptide with C-terminal acetylation and purity of more than 95%.
4. The environmentally friendly leave-in disinfectant hand sanitizer of claim 3, wherein the green high-efficiency peptide S3 mother liquor is prepared from distilled water at a concentration of 2-4mg/ml.
5. The environmentally friendly leave-in sanitizing hand sanitizer of claim 4, wherein the green high-efficiency peptide S3 stock solution is formulated from distilled water at a concentration of 2-3mg/ml.
6. The environmentally friendly leave-in sanitizing hand sanitizer of claim 5, wherein the green high efficiency peptide S3 stock solution is formulated from distilled water at a concentration of 2.8mg/ml.
7. The environmentally friendly leave-in sanitizing hand sanitizer of claim 6, wherein the environmentally friendly leave-in sanitizing hand sanitizer is applied by spraying.
8. The method for preparing the environment-friendly no-clean disinfectant hand sanitizer according to any one of claims 1 to 7, comprising the specific steps of obtaining the green high-efficiency peptide by adopting an Fmoc solid-phase synthesis method, adding distilled water to obtain mother liquor of 2 to 5mg/ml, stirring and premixing a mixture of glycerol and distilled water, vibrating and uniformly mixing with the green high-efficiency peptide S3 solution, and bottling.
9. The method for preparing the environment-friendly no-clean disinfectant hand sanitizer, as set forth in claim 8, wherein the green high-efficiency peptide S3 is added into distilled water, the mass percentage concentration of the green high-efficiency peptide is 0.28%, and the green high-efficiency peptide S3 mother solution is prepared by shaking and mixing for 10 min at 200 rpm under the condition of 4 ℃ in a shaking table.
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| CN114224771B (en) * | 2021-12-31 | 2023-11-17 | 成都佩德生物医药有限公司 | No-wash hand cleanser and preparation method thereof |
| CN114794157A (en) * | 2022-03-29 | 2022-07-29 | 成都佩德生物医药有限公司 | Disinfection spray liquid and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1330714A (en) * | 1998-11-09 | 2002-01-09 | 微科学有限公司 | Virulence genes and proteins and their use |
| JP2003221398A (en) * | 2001-11-22 | 2003-08-05 | Japan Science & Technology Corp | Method for measuring histone deacetylase activity using fluorescent or chromophoric substrate |
| CN103869065A (en) * | 2014-03-28 | 2014-06-18 | 中国农业科学院油料作物研究所 | Aflatoxin M1 nano antibody immunosorbent, immunoaffinity column, preparation method and application thereof |
| CN107556371A (en) * | 2017-10-23 | 2018-01-09 | 安徽科技学院 | The gene of encoding antimicrobial peptide, the gene of fusion protein and recombinant vector |
| CN107648587A (en) * | 2017-10-23 | 2018-02-02 | 安徽科技学院 | The application of antibacterial peptide and fusion protein in the medicine for preparing treatment helicobacter pylori disease |
| CN111349177A (en) * | 2020-03-13 | 2020-06-30 | 西北农林科技大学 | Preparation method and application of fusion antibacterial peptide CAT |
-
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- 2021-07-30 CN CN202110873242.1A patent/CN113520895B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1330714A (en) * | 1998-11-09 | 2002-01-09 | 微科学有限公司 | Virulence genes and proteins and their use |
| JP2003221398A (en) * | 2001-11-22 | 2003-08-05 | Japan Science & Technology Corp | Method for measuring histone deacetylase activity using fluorescent or chromophoric substrate |
| CN103869065A (en) * | 2014-03-28 | 2014-06-18 | 中国农业科学院油料作物研究所 | Aflatoxin M1 nano antibody immunosorbent, immunoaffinity column, preparation method and application thereof |
| CN107556371A (en) * | 2017-10-23 | 2018-01-09 | 安徽科技学院 | The gene of encoding antimicrobial peptide, the gene of fusion protein and recombinant vector |
| CN107648587A (en) * | 2017-10-23 | 2018-02-02 | 安徽科技学院 | The application of antibacterial peptide and fusion protein in the medicine for preparing treatment helicobacter pylori disease |
| CN111349177A (en) * | 2020-03-13 | 2020-06-30 | 西北农林科技大学 | Preparation method and application of fusion antibacterial peptide CAT |
Non-Patent Citations (1)
| Title |
|---|
| NIEDERACHER D.《Characterization of Hex2 protein, a negative regulatory element necessary for glucose repression in yeast》.《European Journal of Biochemistry》.1991,第200卷(第2期),第311-319页. * |
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