CN113633600B - Alcohol-free washing-free disinfection hand sanitizer and preparation method thereof - Google Patents

Abstract

The invention relates to an alcohol-free washing-free disinfection hand sanitizer, which comprises the following main components in percentage by weight: 0.0004 to 0.0032 part of antibacterial peptide, 1 to 4 parts of snake slough extract, 1 to 3 parts of fructus cnidii extract, 1 to 3 parts of radix sophorae flavescentis extract, 1 to 3 parts of aloe vera gel, 0.5 to 3 parts of vitamin E, 0.2 to 0.5 part of carbomer 940, 1 to 4 parts of glycerol, and the balance of purified deionized water, and regulating the pH value to 6.5 to 6.8 by using triethanolamine. The alcohol-free disinfection hand sanitizer has the effects of moisturizing skin and retaining water, is quick-drying and not sticky, can avoid rinsing with clear water, can continuously inhibit bacteria/sterilize, has no irritation to hand skin, can be used for disinfecting wounds and mucous membranes, and has no drug resistance.

Description

Alcohol-free washing-free disinfection hand sanitizer and preparation method thereof

Technical Field

The invention relates to the technical field of antibacterial sterilization, in particular to an alcohol-free washing-free sterilization hand sanitizer and a preparation method thereof.

Background

Skin is an important defending organ of human body, and is the most important barrier of human body against invasion of external biological and physicochemical factors. The skin directly contacts the outside, especially the hands contact various articles, can be infringed by various pollution and harmful factors, and microorganisms polluted on the hands are one of human body infection factors. Therefore, effective hand disinfection measures are taken, and are very important for preventing disease epidemics and controlling the spread of infection, and keeping the health of self and other people. The safety and the sanitation consciousness of people are gradually improved, and the factors of cleaning, health, skin care, bacteriostasis and the like of the hand sanitizer are considered more and more by consumers. Therefore, the sterilization function of the hand sanitizer is changed from an optional function to a common requirement for society, and the antibacterial/antiseptic hand sanitizer is a new important requirement and even part of a long-term requirement.

In 2016, the U.S. Food and Drug Administration (FDA) issued bans on antibacterial washing products, prescribing that certain liquid, gel and foam antibacterial hand washes, body washes and soaps, etc. are not sold, and further prescribing that 28 active ingredients such as triclosan and benzethonium chloride are not suitable for use in hand sanitizer antibacterial products. At present, most hand disinfection and antibiosis products in the market use ethanol as a disinfection active component or other active disinfection components, and the concentration is generally 35-80%. However, the traditional disinfection and antibacterial products have short antibacterial time and poor effect, are irritative to damaged skin, and are easy to cause skin dryness, chapping and desquamation and even dermatitis after being contacted with ethanol for a long time, and recent researches report that pathogenic bacteria resistant to ethanol are generated. Therefore, the traditional disinfection and antibacterial agents cannot meet the pursuit of consumers for safety, so that the focus of people starts to shift to natural antibacterial ingredients, natural extracts such as Chinese herbal medicines and the like are added into the hand sanitizer, and the hand sanitizer is more green and safer. However, the antibacterial effect and the antibacterial speed of the hand sanitizer only added with the antibacterial components of the Chinese herbal medicines are not ideal.

Disclosure of Invention

The invention aims at: the alcohol-free washing-free disinfection hand sanitizer and the preparation method thereof are provided for solving the problems that the existing skin disinfectant has short antibacterial time, poor antibacterial effect and poor follow-up property, and can not be used for disinfecting wounds or mucous membranes.

The technical scheme adopted by the invention is as follows:

an alcohol-free washing-free disinfection hand sanitizer comprises the following main components in percentage by weight: 0.0004 to 0.0032 part of antibacterial peptide, 1 to 4 parts of snake slough extract, 1 to 3 parts of fructus cnidii extract, 1 to 3 parts of radix sophorae flavescentis extract, 1 to 3 parts of aloe vera gel, 0.5 to 3 parts of vitamin E, 0.2 to 0.6 part of carbomer 940, 0.5 to 3 parts of glycerol, and the balance of purified deionized water, and regulating the pH value to be 6.5 to 6.8 by using triethanolamine.

As a further preferred aspect of the invention, an alcohol-free water-free disinfection hand sanitizer comprises the following main components in percentage by weight: 0.0008 to 0.0016 part of antibacterial peptide, 2 to 3 parts of snake slough extract, 1 to 2 parts of fructus cnidii extract, 1 to 2 parts of radix sophorae flavescentis extract, 1 to 2 parts of aloe vera gel, 1 to 2 parts of vitamin E, 0.3 to 0.5 part of carbomer 940, 1 to 3 parts of glycerol and the balance of purified deionized water, and regulating the pH value to be 6.5 to 6.8 by using triethanolamine.

Preferably, the antibacterial peptide is an amphiphilic polypeptide, more preferably an amphiphilic cationic polypeptide.

As a further preferred aspect of the invention, the antimicrobial peptide is one or more of the amphiphilic cationic polypeptides XH-14A (FIKRIARLLRKIFR), XH-14B (FIKRIARLLRKIKR), XH-14C (FIKRIARLLRKIWR), HX-12A (FFRKVLKLIRKI), HX-12B (FFRKVLKLIRKIF), HX-12C (FFRKV LKLIR KIWR), XC-17 (KWKLFKRKIKFLHSAKK), XC-16A (KLLDIVKKVVRAFRSL), XC-16B (KLLDIVKKRVRAFWSL). The polypeptide sequences are SEQ ID NO:1-9.

As a preferred aspect of the present invention, the snake slough extract, cnidium fruit extract and kuh-seng extract are in a specification of 10:1 (mass of raw materials: mass of extract). For example, the snake slough extract is prepared by mixing snake slough and 10 times of water by weight, soaking, decocting with strong fire, then decocting with strong fire, and concentrating to obtain 1-2 times of concentrated solution of snake slough. The extraction methods of the fructus cnidii extract and the radix sophorae flavescentis extract are the same as above.

A preparation method of an alcohol-free washing-free disinfection hand sanitizer comprises the following steps:

(1) Dissolving antibacterial peptide and aloe vera gel in the water solution, stirring to obtain homogeneous solution, performing ultrasonic treatment for 30min, and standing for 6 hr;

(2) Adding periostracum Serpentis extract, fructus Cnidii extract, radix Sophorae Flavescentis extract and vitamin E, and stirring to obtain homogeneous solution;

(3) Adding carbomer 940, and stirring until the carbomer is fully dissolved;

(4) Adding glycerol, and stirring uniformly;

(5) And (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing the corresponding weight with water.

The alcohol-free washing-free disinfection hand cleanser prepared by the invention can be used for rapidly and effectively sterilizing the surface of skin, comprises drug-sensitive bacteria, multi-drug-resistant bacteria, fungi, spores and the like, can be used for disinfecting skin wounds and promoting wound healing, can also be used for disinfecting mucous membrane, and has no drug resistance. Can obviously improve the compliance of people in using the hand disinfectant, reduce the occurrence of bacterial infectious diseases and control the spread and spread of the infectious diseases.

Antibacterial peptides are small molecular polypeptides with biological activity induced in vivo, and are important components of the natural immune system of organisms. More than 3000 antimicrobial peptides have been identified from various species of bacteria, insects, amphibians, fungi, mammals, higher plants and even humans. The antibacterial peptide is generally composed of 6-50 amino acids, and the amphiphilic cationic peptide can selectively interact with the anionic bacterial membrane, so that the antibacterial peptide can kill cells without entering the inside of the cells, and has effects on both drug-sensitive cells and drug-resistant cells; meanwhile, the antibacterial peptide has very rapid action, which also makes cells not easy to generate drug resistance. The antibacterial peptide has the characteristics of wide sources, drug resistance, high efficiency, low toxicity, no drug interaction and the like.

In summary, due to the adoption of the technical scheme, the beneficial effects of the invention are as follows: the amphipathic cationic polypeptide can form an alpha helical structure in a weak acid environment on the surface of skin, and the cell membrane of a thallus is destroyed by a physical action mode so as to achieve the aim of rapid bacteriostasis/sterilization. In addition, the aloe vera gel and the aloe vera gel form a colloid solution in an aqueous solution through hydrogen bonding, hydrophobic effect and the like, and a protective film can be formed on the surface of the skin after the aloe vera gel is smeared on the surface of the skin, so that the antibacterial/bactericidal effect can be sustained on one hand, and the secondary pollution and the wound surface protection can be avoided on the other hand. In addition, the extracts of snake slough, kuh-seng and cnidium fruit are taken as auxiliary materials, so that the synergistic sterilization effect can be achieved, the sterilization effect is improved, the effects of detumescence and itching relieving are achieved, and the glove is suitable for the sterilization treatment of hands which are severely wounded by a large amount of sterilization, washing and glove wearing for a long time of epidemic prevention medical staff.

Drawings

Fig. 1 is a graph showing the bacteriostatic/bactericidal effect of different treatment modes. Wherein A is a sterile water treatment group, B is a commercially available no-clean hand sanitizer (containing ethanol), C is an antibacterial peptide no-clean hand sanitizer (containing no traditional Chinese medicine components), and D is the alcohol-free no-clean disinfection hand sanitizer of example 1.

FIG. 2 is a FT-IR spectrum of CS, HX-12C and CS/HX-12C hand washes.

Detailed Description

The present invention will be described in further detail with reference to the following examples in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the particular embodiments described herein are illustrative only and are not intended to limit the invention, i.e., the embodiments described are merely some, but not all, of the embodiments of the invention. All other embodiments, which can be made by a person skilled in the art without making any inventive effort, are intended to be within the scope of the present invention.

Example 1

1.6mg of antibacterial peptide HX-12C and 2g of aloe vera gel are dissolved in 80mL of aqueous solution, stirred to obtain a homogeneous solution, sonicated for 30min and stood for 6h; adding 3g of snake slough extract, 2g of fructus cnidii extract, 2g of radix sophorae flavescentis extract and 2g of vitamin E, and stirring to obtain a homogeneous solution; adding 0.5g carbomer 940 to disperse in the above solution, standing and swelling to transparent; adding 3g of glycerol and uniformly stirring; and (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing 100g with purified water.

The preparation method of periostracum Serpentis extract comprises mixing periostracum Serpentis and 10 times of water by weight, soaking, decocting with strong fire, and concentrating to obtain 1-2 times of concentrated solution of periostracum Serpentis. The extraction methods of the fructus cnidii extract and the radix sophorae flavescentis extract are the same as the extraction methods.

Example 2

1.2mg of antibacterial peptide HX-12C and 1.5g of aloe vera gel are dissolved in 80mL of aqueous solution, the mixture is stirred to obtain a homogeneous solution, the homogeneous solution is treated by ultrasonic for 30min, and the solution is stood for 6h; adding 2.5g of snake slough extract, 1.5g of fructus cnidii extract, 1.5g of radix sophorae flavescentis extract and 1.5g of vitamin E, and stirring to obtain a homogeneous solution; adding 0.4g carbomer 940 to disperse in the above solution, standing and swelling to transparent; adding 1.5g of glycerol and uniformly stirring; and (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing 100g with purified water. The other steps are the same as in example 1.

Example 3

Dissolving 0.8mg of antibacterial peptide HX-12C and 1g of aloe vera gel in 80mL of aqueous solution, stirring to obtain a homogeneous solution, performing ultrasonic treatment for 30min, and standing for 6h; adding 2g of snake slough extract, 1g of fructus cnidii extract and 1g of radix sophorae flavescentis extract and 1g of vitamin E, and stirring to obtain a homogeneous solution; adding 0.3g carbomer 940 to disperse in the above solution, standing and swelling to transparent; adding 1g of glycerol and uniformly stirring; and (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing 100g with purified water. The other steps are the same as in example 1.

Example 4

1.2mg of antibacterial peptide HX-12A and 1.5g of aloe vera gel are dissolved in 80mL of aqueous solution, the mixture is stirred to obtain a homogeneous solution, the homogeneous solution is treated by ultrasonic for 30min, and the solution is stood for 6h; adding 2.5g of snake slough extract, 1.5g of fructus cnidii extract, 1.5g of radix sophorae flavescentis extract and 1.5g of vitamin E, and stirring to obtain a homogeneous solution; adding 0.4g carbomer 940 to disperse in the above solution, standing and swelling to transparent; adding 1.5g of glycerol and uniformly stirring; and (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing 100g with purified water. The other steps are the same as in example 1.

Example 5

1.2mg of antibacterial peptide HX-12B and 1.5g of aloe vera gel are dissolved in 80mL of aqueous solution, the mixture is stirred to obtain a homogeneous solution, the homogeneous solution is treated by ultrasonic for 30min, and the solution is stood for 6h; adding 2.5g of snake slough extract, 1.5g of fructus cnidii extract, 1.5g of radix sophorae flavescentis extract and 1.5g of vitamin E, and stirring to obtain a homogeneous solution; adding 0.4g carbomer 940 to disperse in the above solution, standing and swelling to transparent; adding 1.5g of glycerol and uniformly stirring; and (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing 100g with purified water. The other steps are the same as in example 1.

Example 6

1.2mg of antibacterial peptide XH-14A and 1.5g of aloe vera gel are dissolved in 80mL of aqueous solution, the mixture is stirred to obtain a homogeneous solution, the homogeneous solution is treated by ultrasonic for 30min, and the solution is stood for 6h; adding 2.5g of snake slough extract, 1.5g of fructus cnidii extract, 1.5g of radix sophorae flavescentis extract and 1.5g of vitamin E, and stirring to obtain a homogeneous solution; adding 0.4g carbomer 940 to disperse in the above solution, standing and swelling to transparent; adding 1.5g of glycerol and uniformly stirring; and (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing 100g with purified water. The other steps are the same as in example 1.

Example 7

1.2mg of antibacterial peptide XH-14B and 1.5g of aloe vera gel are dissolved in 80mL of aqueous solution, the mixture is stirred to obtain a homogeneous solution, the homogeneous solution is treated by ultrasonic for 30min, and the solution is stood for 6h; adding 2.5g of snake slough extract, 1.5g of fructus cnidii extract, 1.5g of radix sophorae flavescentis extract and 1.5g of vitamin E, and stirring to obtain a homogeneous solution; adding 0.4g carbomer 940 to disperse in the above solution, standing and swelling to transparent; adding 1.5g of glycerol and uniformly stirring; and (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing 100g with purified water. The other steps are the same as in example 1.

Example 8

1.2mg of antibacterial peptide XH-14C and 1.5g of aloe vera gel are dissolved in 80mL of aqueous solution, the mixture is stirred to obtain a homogeneous solution, the homogeneous solution is treated by ultrasonic for 30min, and the solution is stood for 6h; adding 2.5g of snake slough extract, 1.5g of fructus cnidii extract, 1.5g of radix sophorae flavescentis extract and 1.5g of vitamin E, and stirring to obtain a homogeneous solution; adding 0.4g carbomer 940 to disperse in the above solution, standing and swelling to transparent; adding 1.5g of glycerol and uniformly stirring; and (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing 100g with purified water. The other steps are the same as in example 1.

Example 9

1.2mg of antibacterial peptide XC-17 and 1.5g of aloe vera gel are dissolved in 80mL of aqueous solution, and are stirred to obtain a homogeneous solution, sonicated for 30min and stood for 6h; adding 2.5g of snake slough extract, 1.5g of fructus cnidii extract, 1.5g of radix sophorae flavescentis extract and 1.5g of vitamin E, and stirring to obtain a homogeneous solution; adding 0.4g carbomer 940 to disperse in the above solution, standing and swelling to transparent; adding 1.5g of glycerol and uniformly stirring; and (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing 100g with purified water. The other steps are the same as in example 1.

Example 10

1.2mg of antibacterial peptide XC-16A and 1.5g of aloe vera gel are dissolved in 80mL of aqueous solution, the mixture is stirred to obtain a homogeneous solution, the homogeneous solution is treated by ultrasonic for 30min, and the solution is stood for 6h; adding 2.5g of snake slough extract, 1.5g of fructus cnidii extract, 1.5g of radix sophorae flavescentis extract and 1.5g of vitamin E, and stirring to obtain a homogeneous solution; adding 0.4g carbomer 940 to disperse in the above solution, standing and swelling to transparent; adding 1.5g of glycerol and uniformly stirring; and (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing 100g with purified water. The other steps are the same as in example 1.

Example 11

1.2mg of antibacterial peptide XC-16B and 1.5g of aloe vera gel are dissolved in 80mL of aqueous solution, the mixture is stirred to obtain a homogeneous solution, the homogeneous solution is treated by ultrasonic for 30min, and the solution is stood for 6h; adding 2.5g of snake slough extract, 1.5g of fructus cnidii extract, 1.5g of radix sophorae flavescentis extract and 1.5g of vitamin E, and stirring to obtain a homogeneous solution; adding 0.4g carbomer 940 to disperse in the above solution, standing and swelling to transparent; adding 1.5g of glycerol and uniformly stirring; and (3) dripping triethanolamine to ensure that the pH value of the hand washing liquid is between 6.5 and 6.8, and supplementing 100g with purified water. The other steps are the same as in example 1.

The killing conditions and the using effects of the products of examples 1 to 11 on microorganisms (natural bacteria on hands) are shown in the following table 1:

table 1 table of effect of use

Example 12

The method is characterized in that 48 testees are randomly selected to be divided into 4 groups, 12 people in each group are respectively treated with sterile water for washing hands as a control group, and the commercial no-wash hand sanitizer (containing 75% ethanol) and the self-made antibacterial peptide hand sanitizer (the alcohol-free no-wash disinfection hand sanitizer without traditional Chinese medicine components, namely, the snake slough extract, the fructus cnidii extract and the radix sophorae flavescentis extract are removed) are respectively treated, and compared with the examples, after the preparation method is left for 6 hours, 2g of vitamin E is added and stirred to obtain a homogeneous solution, and other hand sanitizer effects of the alcohol-free no-wash disinfection hand sanitizer in the examples 1 are measured. The specific method comprises the following steps: a set of sterilized nutrient agar plates is dispensed to 48 testees, and the thumb, index finger and middle finger of one hand are used for printing on the plates before hand washing, and then hand washing is carried out according to different requirements. Wherein the control group is rinsed with sterile water for 1min; after three groups of the commercially available no-wash hand cleaner, the self-made antibacterial peptide hand cleaner (without traditional Chinese medicine components) and the alcohol-free no-wash disinfection hand cleaner of the embodiment 1 of the application rub hands with 2mL of the hand cleaner for 1min, each 3 people respectively wait 1min, 5min, 10min and 30min and then press finger marks on the same flat plate by thumb, index finger and middle finger of the same hand. The plate is covered with a cover, marked and placed upside down into a biochemical incubator at 37 ℃ for culturing for 48 hours. After 48 hours, the plates were removed and counted for bacterial colony Count (CFU) before and after washing the hands, and the results are shown in table 2 and fig. 1. As can be seen from the data in Table 2, the four treatment modes have certain antibacterial effects after treatment, and the antibacterial effects gradually decline after 5min of rubbing and washing of the commercially available hand-washing liquid (containing ethanol), because the ethanol is volatilized directly after rubbing and washing, and the continuous antibacterial capability is not achieved. The alcohol-free washing-free disinfection hand sanitizer of the embodiment 1 has good antibacterial capacity after 30min of washing, and even the colony number is less than 1, namely, the disinfection hand sanitizer is not detected. The aloe gel and the antibacterial peptide in the antibacterial peptide no-wash hand cleanser can form a layer of film on the surface of skin, and have the effect of continuous bacteriostasis.

Table 2 shows the effect of the hand-washing liquid compared with the commercially available hand-washing liquid

Example 13

The structures of aloe vera gel powder, antibacterial peptide HX-12C powder, and lyophilized powder of the mixed solution of aloe vera gel and antibacterial peptide HX-12C were examined by Nicolet iS10 Fourier transform infrared absorption spectroscopy (FT-IR) after tabletting with potassium bromide, and the results are shown in FIG. 2. 1730cm in infrared spectrogram of aloe vera gel ^-1 And 1600cm ^-1 The strong absorption peak at the position is a telescopic absorption peak of carboxyl (COO-); 1024cm ^-1 The absorption peak at the position is the stretching vibration of the C-O bond on the sugar ring framework; at 1154 and 1154 cm ^-1 The absorption peak at this point is a characteristic peak of glycosidic linkage, caused by asymmetric C-O-C stretching vibration. 1654cm in the IR spectrum of the antimicrobial peptide HX-12C ^-1 Alpha spiral infrared spectrum absorption at 1627cm ^-1 Is absorbed by beta-sheet infrared spectrum, 1538 and 1538cm ^-1 The amide II absorption peak is not an amide III absorption peak. 1654cm of the antimicrobial peptide HX-12C after the aloe vera gel is blended with the antimicrobial peptide HX-12C ^-1 The absorption of the alpha helix infrared spectrum at the site is reduced and 1627cm ^-1 The absorption of beta-sheet infrared spectrum is enhanced, which shows that the antibacterial peptide HX-12C mainly adopts an alpha helical structure, and the absorption peak of the amide II is 1538cm ^-1 Red shift to 1548 cm ^-1 Indicating that the conformation of the polypeptide was also changed. Thus, the interaction exists between the aloe vera gel and the antibacterial peptide HX-12C, and the conformation and secondary structure of the antibacterial peptide can be obviously changed.

Those of ordinary skill in the art will appreciate that: the discussion of any of the embodiments above is merely exemplary and is not intended to obscure the scope of the disclosure, including the claims, being limited to these examples; the technical features of the above embodiments or in the different embodiments may also be combined under the concepts of the present disclosure, the steps may be implemented in any order, and there are many other variations of the different aspects of one or more embodiments in this application as described above, which are not provided in detail for the sake of brevity.

The present application is intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Accordingly, any omissions, modifications, equivalents, improvements and the like, which are within the spirit and principles of the one or more embodiments in the present application, are intended to be included within the scope of the present disclosure.

<110> Hunan academy of science and technology

<120> an alcohol-free washing-free hand sanitizer and preparation method thereof

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Claims (3)

1. The alcohol-free water-free disinfection hand sanitizer is characterized by comprising the following components in parts by weight: 0.0004-0.0032 parts of antibacterial peptide, 1-4 parts of snake slough extract, 1-3 parts of fructus cnidii extract, 1-3 parts of radix sophorae flavescentis extract, 1-3 parts of aloe vera gel, 0.5-3 parts of vitamin E, 0.2-0.6 part of carbomer 940, 0.5-3 parts of glycerol and the balance of deionized water, and regulating the pH value to be 6.5-6.8 by using triethanolamine; the antibacterial peptide is an amphipathic cationic polypeptide;

the amphipathic cationic polypeptide is HX-12C, and the sequence is as follows: FFRKVLKLIRKIWR;

the snake slough extract, the cnidium fruit extract and the kuh-seng extract are respectively water extracts obtained by mixing and extracting the snake slough, the cnidium fruit and the kuh-seng with water of 10 times of the weight of the snake slough, the cnidium fruit and the kuh-seng.

2. The alcohol-free water-free disinfectant hand sanitizer of claim 1, wherein the alcohol-free water-free disinfectant hand sanitizer comprises the following components in parts by weight: 0.0008-0.0016 part of antibacterial peptide, 2-3 parts of snake slough extract, 1-2 parts of fructus cnidii extract, 1-2 parts of radix sophorae flavescentis extract, 1-2 parts of aloe vera gel, 1-2 parts of vitamin E, 0.3-0.5 part of carbomer 940, 1-3 parts of glycerol and the balance of deionized water, and regulating the pH value to 6.5-6.8 by using triethanolamine.

3. A method of preparing the alcohol-free, water-free, sanitizing hand sanitizer of claim 1 or 2, comprising the steps of:

(1) Dissolving antibacterial peptide and aloe vera gel in the water solution, stirring to obtain homogeneous solution, performing ultrasonic treatment for 30min, and standing for 6 hr;

(2) Adding periostracum Serpentis extract, fructus Cnidii extract, radix Sophorae Flavescentis extract and vitamin E, and stirring to obtain homogeneous solution;

(3) Adding carbomer 940, and stirring until the carbomer is fully dissolved;

(4) Adding glycerol, and stirring uniformly;

(5) And (3) dropwise adding triethanolamine to enable the pH value of the hand washing liquid to be 6.5-6.8, and supplementing corresponding weight with water.