CN113490843B - 采用拉曼光谱确定病毒滴度的方法 - Google Patents

采用拉曼光谱确定病毒滴度的方法 Download PDF

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CN113490843B
CN113490843B CN201980092894.2A CN201980092894A CN113490843B CN 113490843 B CN113490843 B CN 113490843B CN 201980092894 A CN201980092894 A CN 201980092894A CN 113490843 B CN113490843 B CN 113490843B
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wavenumber ranges
wavenumber
viral
ranges
titer
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CN113490843A (zh
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J·丘奇韦尔
M·O·巴拉迪兹
D·马歇尔
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Cell Therapy Catapult Ltd
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F17/00—Digital computing or data processing equipment or methods, specially adapted for specific functions
    • G06F17/10—Complex mathematical operations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/129—Using chemometrical methods
    • G01N2201/1293—Using chemometrical methods resolving multicomponent spectra
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/129—Using chemometrical methods
    • G01N2201/1296—Using chemometrical methods using neural networks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses

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  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201980092894.2A 2018-12-24 2019-12-23 采用拉曼光谱确定病毒滴度的方法 Active CN113490843B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1821206.8 2018-12-24
GB1821206.8A GB2580186B (en) 2018-12-24 2018-12-24 Monitoring viral titre using Raman Spectroscopy
PCT/GB2019/053695 WO2020136376A1 (en) 2018-12-24 2019-12-23 Methods for determining viral titre using raman spectroscopy

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CN113490843A CN113490843A (zh) 2021-10-08
CN113490843B true CN113490843B (zh) 2024-08-09

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US (2) US11703455B2 (https=)
EP (1) EP3903096A1 (https=)
JP (1) JP7522114B2 (https=)
KR (1) KR20210104887A (https=)
CN (1) CN113490843B (https=)
AU (1) AU2019412791A1 (https=)
CA (1) CA3123168A1 (https=)
GB (1) GB2580186B (https=)
IL (1) IL284283B2 (https=)
WO (1) WO2020136376A1 (https=)

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CN110609030B (zh) * 2019-10-22 2022-02-25 上海海关动植物与食品检验检疫技术中心 一种基于检出概率模型的拉曼快检性能综合评价方法
GB202010104D0 (en) * 2020-07-01 2020-08-12 Cell Therapy Catapult Ltd Method
EP4083852A1 (en) * 2021-04-30 2022-11-02 Universidad de Sevilla Virus detection and quantification method in fluid samples by digital processing diffuse optical reflectance hyperspectral images obtained in the visible and near infrared ranges
US12422367B2 (en) 2021-11-19 2025-09-23 Janssen Biotech, Inc. Methods for determination of virus titer in a sample using Raman spectroscopy
CN114628037A (zh) * 2022-02-25 2022-06-14 上海应用技术大学 一种基于深度学习卷积神经网络的新冠病毒识别预测模型
CN119256220A (zh) 2022-05-23 2025-01-03 豪夫迈·罗氏有限公司 用于区分aav颗粒血清型和aav颗粒荷载状态的基于拉曼的方法

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US12135289B2 (en) 2024-11-05
US20230384228A1 (en) 2023-11-30
JP7522114B2 (ja) 2024-07-24
GB201821206D0 (en) 2019-02-06
EP3903096A1 (en) 2021-11-03
US11703455B2 (en) 2023-07-18
AU2019412791A1 (en) 2021-07-08
IL284283A (en) 2021-08-31
CA3123168A1 (en) 2020-07-02
IL284283B1 (en) 2024-03-01
GB2580186A (en) 2020-07-15
IL284283B2 (en) 2024-07-01
GB2580186B (en) 2021-09-15
CN113490843A (zh) 2021-10-08
KR20210104887A (ko) 2021-08-25
JP2022515417A (ja) 2022-02-18
WO2020136376A1 (en) 2020-07-02
US20220018782A1 (en) 2022-01-20

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