CN113481326A - 一种等温核酸扩增反应试剂、等温核酸扩增方法及其应用 - Google Patents
一种等温核酸扩增反应试剂、等温核酸扩增方法及其应用 Download PDFInfo
- Publication number
- CN113481326A CN113481326A CN202110780940.7A CN202110780940A CN113481326A CN 113481326 A CN113481326 A CN 113481326A CN 202110780940 A CN202110780940 A CN 202110780940A CN 113481326 A CN113481326 A CN 113481326A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- reaction reagent
- acid amplification
- isothermal nucleic
- amplification reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 104
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 70
- 230000003321 amplification Effects 0.000 title claims abstract description 62
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 58
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 58
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 58
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 54
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 18
- 102100034343 Integrase Human genes 0.000 claims abstract description 10
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims abstract description 10
- 229930006000 Sucrose Natural products 0.000 claims abstract description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 8
- 239000005720 sucrose Substances 0.000 claims abstract description 8
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229920001213 Polysorbate 20 Polymers 0.000 claims abstract description 6
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 6
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 6
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims abstract description 6
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims abstract description 6
- 238000001514 detection method Methods 0.000 claims description 62
- 241000725303 Human immunodeficiency virus Species 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 18
- 239000007850 fluorescent dye Substances 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 15
- 239000000523 sample Substances 0.000 claims description 14
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 9
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 9
- 241000725643 Respiratory syncytial virus Species 0.000 claims description 8
- 244000000010 microbial pathogen Species 0.000 claims description 8
- 239000002696 acid base indicator Substances 0.000 claims description 7
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 claims description 5
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical group C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 claims description 5
- 229960003531 phenolsulfonphthalein Drugs 0.000 claims description 5
- 241000713869 Moloney murine leukemia virus Species 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 241000713772 Human immunodeficiency virus 1 Species 0.000 claims description 2
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims description 2
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- -1 sanskanting Substances 0.000 claims description 2
- 244000052769 pathogen Species 0.000 abstract description 11
- 239000004094 surface-active agent Substances 0.000 abstract description 8
- 230000005764 inhibitory process Effects 0.000 abstract description 7
- 239000007853 buffer solution Substances 0.000 abstract description 4
- 210000004369 blood Anatomy 0.000 abstract description 3
- 239000008280 blood Substances 0.000 abstract description 3
- 210000002345 respiratory system Anatomy 0.000 abstract description 3
- 230000001624 sedative effect Effects 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 23
- 239000013558 reference substance Substances 0.000 description 22
- 238000011901 isothermal amplification Methods 0.000 description 10
- 238000006073 displacement reaction Methods 0.000 description 8
- 238000003745 diagnosis Methods 0.000 description 7
- 238000003752 polymerase chain reaction Methods 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 239000012535 impurity Substances 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 238000005336 cracking Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 230000008859 change Effects 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 230000004907 flux Effects 0.000 description 4
- 238000007901 in situ hybridization Methods 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241001112090 Pseudovirus Species 0.000 description 3
- 239000000443 aerosol Substances 0.000 description 3
- 230000006037 cell lysis Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 239000000539 dimer Substances 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012175 pyrosequencing Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 241000712431 Influenza A virus Species 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 206010057040 Temperature intolerance Diseases 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000013211 curve analysis Methods 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000005861 gene abnormality Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000008543 heat sensitivity Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 230000005758 transcription activity Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- AIDS & HIV (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
参考品编号 | 浓度 | 详细信息 |
RSV L1 | 200copies/mL | RSV假病毒 |
RSV L2 | 500copies/mL | RSV假病毒 |
参考品编号 | 浓度 | 基因型 |
RSV T1 | 2000copies/mL | RSV假病毒 |
RSV T2 | 5000copies/mL | RSV假病毒 |
RSV T3 | / | 正常人咽拭子液(RSV阴性) |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110780940.7A CN113481326B (zh) | 2021-07-09 | 2021-07-09 | 一种等温核酸扩增反应试剂、等温核酸扩增方法及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110780940.7A CN113481326B (zh) | 2021-07-09 | 2021-07-09 | 一种等温核酸扩增反应试剂、等温核酸扩增方法及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN113481326A true CN113481326A (zh) | 2021-10-08 |
CN113481326B CN113481326B (zh) | 2022-11-22 |
Family
ID=77938585
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110780940.7A Active CN113481326B (zh) | 2021-07-09 | 2021-07-09 | 一种等温核酸扩增反应试剂、等温核酸扩增方法及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113481326B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115786450A (zh) * | 2022-12-27 | 2023-03-14 | 广东优尼德生物科技有限公司 | 一种1,5-脱水-d-山梨醇测定试剂盒及其制备方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007300932A (ja) * | 2000-12-26 | 2007-11-22 | Takara Bio Inc | 病原微生物の検出方法 |
CN102352350A (zh) * | 2011-09-30 | 2012-02-15 | 北京万达因生物医学技术有限责任公司 | 一种同序引物置换的核酸扩增技术 |
WO2014030066A2 (en) * | 2012-08-22 | 2014-02-27 | Bernitz Mats Nilsson | Methods for identifying nucleic acid sequences |
CN112080583A (zh) * | 2020-05-29 | 2020-12-15 | 复旦大学 | 一种免提取等温检测新型冠状病毒的试剂盒 |
-
2021
- 2021-07-09 CN CN202110780940.7A patent/CN113481326B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007300932A (ja) * | 2000-12-26 | 2007-11-22 | Takara Bio Inc | 病原微生物の検出方法 |
CN102352350A (zh) * | 2011-09-30 | 2012-02-15 | 北京万达因生物医学技术有限责任公司 | 一种同序引物置换的核酸扩增技术 |
WO2014030066A2 (en) * | 2012-08-22 | 2014-02-27 | Bernitz Mats Nilsson | Methods for identifying nucleic acid sequences |
CN112080583A (zh) * | 2020-05-29 | 2020-12-15 | 复旦大学 | 一种免提取等温检测新型冠状病毒的试剂盒 |
Non-Patent Citations (1)
Title |
---|
孟兆祥等: "一种DNA扩增的新技术: 利用热稳定的Bst DNA聚合酶驱动跨越式滚环等温扩增反应", 《中国生物化学与分子生物学报》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115786450A (zh) * | 2022-12-27 | 2023-03-14 | 广东优尼德生物科技有限公司 | 一种1,5-脱水-d-山梨醇测定试剂盒及其制备方法 |
CN115786450B (zh) * | 2022-12-27 | 2023-11-17 | 广东优尼德生物科技有限公司 | 一种1,5-脱水-d-山梨醇测定试剂盒及其制备方法 |
Also Published As
Publication number | Publication date |
---|---|
CN113481326B (zh) | 2022-11-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN110358815B (zh) | 一种同时检测多个靶标核酸的方法及其试剂盒 | |
CN113355459B (zh) | 一种检测筛查新冠病毒n501y突变的方法及试剂盒 | |
CN103409552B (zh) | 同步检测多种高危型hpv基因型的引物组、探针组、方法及试剂盒 | |
CN113652505B (zh) | 检测新型冠状病毒及其voc-202012/01突变株的方法和试剂盒 | |
CN103614489A (zh) | 一种登革热病毒的恒温扩增检测试剂盒及检测方法 | |
EP1713925B1 (en) | Primers and probes for the detection of hiv | |
US9617606B2 (en) | Oligonucleotide for HIV detection, HIV detection kit, and HIV detection method | |
CN110305988A (zh) | 鸽新城疫病毒LAMP-TaqMan检测试剂盒 | |
EP3214181B1 (en) | Oligonucleotides, set of oligonucleotides, htlv-i/htlv-ii infection diagnostic and discrimination kit, polynucleotide suitable as reference target for designing primers and probes for the detection and differentiation of htlv-i and htlv-ii, amplicon and method for detecting at least one htlv target | |
CN113481326B (zh) | 一种等温核酸扩增反应试剂、等温核酸扩增方法及其应用 | |
CN110923361A (zh) | 基于数字pcr用于血源筛查的引物、探针及试剂盒 | |
KR102019804B1 (ko) | 중증 열성 혈소판 감소 증후군 바이러스 검출용 프라이머 세트 및 검출방법 | |
CN114729347A (zh) | 未分化标记基因高灵敏度检测法 | |
CN102399903B (zh) | 一种基孔肯雅病毒等温扩增检测试剂盒及其引物 | |
CN110894550A (zh) | 鳗鲡疱疹病毒(hva)的raa恒温荧光检测方法及试剂 | |
CN115029345A (zh) | 基于crispr的核酸检测试剂盒及其应用 | |
KR102076343B1 (ko) | 실시간 lamp법을 이용한 아데노바이러스 55형 검출용 조성물 및 이의 용도 | |
CN113832259A (zh) | 一种新型冠状病毒快速检测试剂盒及其应用 | |
WO2013049822A2 (en) | Diagnostic method for determining animals persistently infected (pi) with bovine viral diarrhea virus (bvdv) | |
CN106191314B (zh) | 一种dna病毒的lamp检测试剂盒、检测方法及应用 | |
CN115058493B (zh) | 一种用于多重核酸检测的dna探针、crispr-反向点杂交核酸检测系统及应用 | |
CN112626263B (zh) | 酶切探针恒温检测呼吸道感染真菌病原体的试剂盒 | |
CN110894551A (zh) | 草鱼出血病i型病毒(gcrv-i)的raa恒温荧光检测方法及试剂 | |
CN115595382B (zh) | 用于检测猪肠道冠状病毒的引物组及其应用 | |
CN110894549A (zh) | 流行性造血器官坏死病毒(ehnv)的raa恒温荧光检测方法及试剂 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A Isothermal Nucleic Acid Amplification Reaction Reagent, Isothermal Nucleic Acid Amplification Method and Its Application Effective date of registration: 20230505 Granted publication date: 20221122 Pledgee: China Everbright Bank Limited by Share Ltd. Xiamen branch Pledgor: ANBIO (XIAMEN) PRODUCTS Inc. Registration number: Y2023980039764 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20221122 Pledgee: China Everbright Bank Limited by Share Ltd. Xiamen branch Pledgor: ANBIO (XIAMEN) PRODUCTS Inc. Registration number: Y2023980039764 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: An isothermal nucleic acid amplification reaction reagent, isothermal nucleic acid amplification method and its application Granted publication date: 20221122 Pledgee: Industrial Bank Limited by Share Ltd. Xiamen branch Pledgor: ANBIO (XIAMEN) PRODUCTS Inc. Registration number: Y2024980029646 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |