CN113456518A - Composite polypeptide mouthwash and preparation method thereof - Google Patents
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Abstract
The invention belongs to the application field of mouthwash, and particularly relates to a compound polypeptide mouthwash and a preparation method thereof, wherein the mouthwash comprises biological antibacterial peptide, melittin and biological lysozyme, and the three are combined for use to achieve a synergistic effect, so that the mouthwash can kill helicobacter pylori, oral bacteria and pathogenic bacteria simultaneously, and has triple functions of killing oral pathogenic bacteria, deodorizing and treating oral cavity; the medicine does not generate drug resistance, has no side effect, is safer, and can be used by pregnant women and children; and has antiinflammatory, antibacterial, anticancer, radioprotective, platelet aggregation inhibiting, calcium utilization promoting, and dental caries preventing effects.
Description
Technical Field
The invention belongs to the field of mouthwash application, and particularly relates to a compound polypeptide mouthwash and a preparation method thereof.
Background
With the improvement of living standard of people and the continuous pursuit of beauty, oral hygiene has become one of the focuses of modern people. The oral hygiene care is not limited to brushing twice in the morning and at night, and carrying the mouthwash with the mouth is fashionable, and the mouth wash plays an increasingly important role in daily life. The ingredients with the bactericidal effect are added into the mouthwash, so that the bactericidal effect of the mouthwash can be improved, the formation of bacteria in the oral cavity can be inhibited, the risk of various oral diseases is reduced, and the oral health is better cared; the antibacterial component in the mouthwash in the market at present is mainly lysomycete, has single effect, and particularly has not ideal bactericidal effect on most gram-negative bacteria; however, the mouthwash with various antibacterial effects can bring other side effects and harm human health.
Patent document 99816880.7 discloses an oral cavity cleanser composition containing tea polyphenol for removing halitosis and refreshing breath, but the oral cavity cleanser composition has the disadvantages of single additive component and limited effect, so that the mouth wash is difficult to be endowed with multiple efficacies.
Patent document CN105411914A discloses a mouthwash, which comprises the following raw materials by mass percent: pseudo-ginseng: 8-10%, wild chrysanthemum flower: 6-8% of honeysuckle flower: 8-12%, mint: 3-5% and chlorhexidine: 3-5% and essential oil: 10-15%, polyoxyethylene hydrogenated castor oil: 3-6%, sodium monofluorophosphate: 0.1-0.2%, deionized water: 38 to 45 percent. The collutory has effects in preventing dental bacteria, eliminating halitosis, clearing away heat and toxic materials, relieving swelling, stopping bleeding, and preventing sensitivity. However, chlorhexidine contained in the mouthwash can cause blood pressure to rise, thereby increasing the probability of heart disease and stroke, and the like.
Patent document CN103006525A discloses a mouth wash for removing smoke spots, which comprises mineralized water, additive active ingredients and Chinese herbal medicine extracts, wherein the active ingredients comprise 0.1 part of dextranase, 0.2 part of protease, 0.2 part of sodium hydroxide and 0.05 part of sodium benzoate, the Chinese herbal medicine extracts comprise 3-6 parts of equisetum hiemale, 3-6 parts of black fish bone, 2-3 parts of pseudo-ginseng, 2-3 parts of honeysuckle, 2-3 parts of chrysanthemum, 2-3 parts of Chinese olive, 2-3 parts of borneol, 3-4 parts of cacumen biotae and 2-3 parts of radix ophiopogonis, and the additives comprise propolis, ethanol, mint, vitamins, clove oil, lemon oil and the like. The mouthwash in the invention can remove the smoke smell of the oral cavity and the smoke stain of the teeth, but the mouthwash contains sodium benzoate, as is known, the sodium benzoate reacts with gastric acid to produce benzoic acid, the benzoic acid has certain toxicity, and can cause chronic benzene poisoning of people after long-term drinking, and although trace amount of sodium benzoate is not harmful to human bodies at present, the situation after long-term use is still uncertain.
Aiming at the problems of single antibacterial effect, large side effect, poor taste and the like of the existing mouthwash, the mouthwash with multiple antibacterial effects, good curative effect, small side effect and good taste needs to be searched urgently, and the public demand is met while the good oral protection effect is obtained.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the compound polypeptide mouthwash which can kill helicobacter pylori, oral bacteria and pathogenic bacteria simultaneously, has triple functions of killing oral pathogenic bacteria, deodorizing and treating oral cavity, does not generate drug resistance, has no side effect, is safer, and solves the problems of single effect and great side effect of the existing mouthwash.
The invention provides a compound polypeptide mouthwash, which comprises raw material components of biological components, traditional Chinese medicine components and pH buffer solution, wherein the natural biological components comprise biological antibacterial peptide, melittin, casein phosphopeptide, biological lysozyme, chitosan and natamycin, and the traditional Chinese medicine components comprise honeysuckle and isatis root.
Preferably, the natural biological component comprises the following components in parts by weight: 0.1-10 parts of biological antibacterial peptide, 0.01-1 part of melittin, 0.2-1 part of casein phosphopeptide, 0.2-1 part of biological lysozyme, 0.2-2 parts of chitosan and 0.1-1 part of natamycin.
Preferably, the traditional Chinese medicine components comprise the following components in parts by weight: 0.1-2 parts of honeysuckle and 0.1-2 parts of isatis root.
Preferably, the pH buffer is a phosphate buffer or a carbonate buffer, and the weight percentage of the pH buffer in the mouthwash is 80-98.99%.
The invention also provides a preparation method of the compound polypeptide mouthwash, which comprises the following steps: adding the biological antibacterial peptide, melittin, casein phosphopeptide, biological lysozyme, chitosan, natamycin, honeysuckle, radix isatidis and pH buffer solution into distilled water, and uniformly stirring and mixing to obtain the compound polypeptide mouthwash.
Compared with the prior art, the invention has the beneficial effects that:
1. the biological antibacterial peptide has the effects of regulating the metabolism of a human body and regulating physiological functions, can promote digestion, has the effect of enhancing immunity, and has the effects of resisting bacteria and viruses, reducing blood pressure and blood fat and the like; the biological antimicrobial peptides are capable of killing gram negative and gram positive bacteria (including those resistant to conventional antibiotics), mycobacteria (including tuberculosis), pallidoviruses, fungi, and even transformed or cancerous cells. Unlike many traditional antibiotics, antimicrobial peptides can also act as immunomodulators to improve immunity. In addition to killing bacteria directly, antimicrobial peptides also have some immunomodulatory functions that can be involved in the clearance of infection, including the ability to alter host gene expression, act as chemokines-like or induce chemokine production, inhibit endotoxin-induced inflammatory cytokine production, promote wound healing, not only clear infection but also prevent infection. Compared with the traditional antibiotics, the antibacterial peptide seems to have no drug resistance, has a wide bactericidal spectrum and short bactericidal time. Has strong killing effect on part of fungi, protozoa, viruses, cancer cells, etc.
2. The melittin has good oxidation resistance, has the effects of resisting inflammation, sterilization, cancer and radiation, inhibiting platelet aggregation and the like, also has the effects of inhibiting and killing various bacteria, is prepared into natural chewing gum after being specially processed, and can play the roles of protecting teeth and eliminating tartar.
3. The casein phosphopeptide can promote the absorption and utilization of calcium, iron, zinc, magnesium and other mineral substances, increase the bone mass, effectively improve the intake amount, the absorption and the utilization rate of calcium, iron, zinc and other divalent mineral substances of a human body, has the effects of strengthening teeth, strengthening teeth and repairing gum and teeth, and has no toxic or side effect.
4. The biological lysozyme has the effects of resisting bacteria, resisting viruses, resisting tumors, stopping bleeding, relieving swelling and pain, accelerating the tissue recovery function and the like, and is a natural anti-infective substance with the bactericidal effect.
5. The chitosan of the invention has the functions of strengthening immunity, inhibiting aging, preventing diseases, resisting cancers, inhibiting the metastasis of cancer cells, promoting the healing of diseases and regulating the physiological function of human bodies, is a natural preservative and has stronger inhibiting effect on various bacteria.
6. The natamycin can inhibit fungi and prevent the generation of mycotoxin; the natamycin is harmless to human body, is difficult to be absorbed by digestive tract of human body, is difficult to generate resistance to the natamycin by microorganism, and can be used for treating fungal infection including candida, aspergillus, fusarium and the like due to the characteristics of low solubility and the like.
7. The honeysuckle has the main effects of clearing heat and removing toxicity and is mainly used for treating epidemic febrile disease fever, heat toxin and bloody dysentery, carbuncle, cellulitis, furunculosis and the like; the honeysuckle contains chlorogenic acid, luteolin glycoside and other pharmacological active ingredients, has strong inhibitory effect on hemolytic streptococcus, staphylococcus aureus and other pathogenic bacteria and upper respiratory infection pathogenic viruses, and has the effects of enhancing immunity, resisting early pregnancy, protecting liver, resisting tumor, diminishing inflammation, relieving fever, stopping bleeding (blood coagulation), inhibiting intestinal absorption of cholesterol and the like.
8. The isatis root has the effects of clearing away heat and toxic materials, cooling blood and relieving sore throat, is mainly used for treating exogenous fever, early epidemic febrile disease, sore throat, epidemic warm macula, mumps, erysipelas and carbuncle swelling sore toxin, and has an inhibiting effect on various gram-positive bacteria, gram-negative bacteria, influenza viruses, arbovirus and parotid viruses; can enhance the immune function; has obvious antipyretic effect.
9. The compound polypeptide mouthwash provided by the invention adopts a compound polypeptide technology, is the mouthwash capable of killing helicobacter pylori and oral bacteria and pathogenic bacteria, and has core components of biological antibacterial peptide, melittin and biological lysozyme, is an active amino acid chain with higher content, is an intermediate product for degrading protein into amino acid, and has unique physiological activity and medical health care effect which are not possessed by original protein and monomer amino acid; the biological antibacterial peptide can kill gram-negative bacteria, gram-positive bacteria, mycobacteria, pallium viruses and fungi, the melittin has the effects of resisting inflammation, bacteria and radiation, inhibiting and killing various bacteria, inhibiting platelet aggregation and the like, and the biological lysozyme has the effects of resisting bacteria, viruses and tumors, stopping bleeding, relieving swelling and pain, accelerating the tissue recovery function and the like; the three components are combined to enhance the sterilization effect, have the synergistic effect, dissolve bacteria by dissolving bacterial cell walls, simply speaking, directly penetrate through a protective layer outside the bacteria, go deep into the interior of enemy troops and kill the bacteria; has the triple functions of killing pathogenic bacteria in the oral cavity, deodorizing the oral cavity and treating.
10. Compared with the common mouthwash and the therapeutic mouthwash on the market, the compound polypeptide mouthwash provided by the invention can kill helicobacter pylori, oral bacteria and pathogenic bacteria and improve the problem of black and yellow teeth, has no harm to human bodies, reduces the oral bacteria through biological antibacterial peptide, melittin and biological lysozyme, does not generate drug resistance, has no side effect, is safer, and can be used by pregnant women and children; and has antiinflammatory, antibacterial, anticancer, radioprotective, platelet aggregation inhibiting, calcium utilization promoting, and dental caries preventing effects.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A preparation method of a compound polypeptide mouthwash comprises the following steps:
adding 0.1g of biological antibacterial peptide, 0.01g of melittin, 0.2g of casein phosphopeptide, 0.2g of biological lysozyme, 0.2g of chitosan, 0.1g of natamycin, 0.1g of honeysuckle, 0.1g of radix isatidis and 5g of carbonate buffer solution into distilled water, and uniformly stirring and mixing to obtain the compound polypeptide mouthwash.
Example 2
A preparation method of a compound polypeptide mouthwash comprises the following steps:
adding 1g of biological antibacterial peptide, 0.1g of melittin, 0.5g of casein phosphopeptide, 0.4g of biological lysozyme, 0.5g of chitosan, 0.2g of natamycin, 0.2g of honeysuckle, 0.2g of radix isatidis and 20g of carbonate buffer solution into distilled water, and uniformly stirring and mixing to obtain the compound polypeptide mouthwash.
Example 3
A preparation method of a compound polypeptide mouthwash comprises the following steps:
adding 5g of biological antibacterial peptide, 0.3g of melittin, 0.4g of casein phosphopeptide, 0.5g of biological lysozyme, 1g of chitosan, 0.4g of natamycin, 0.5g of honeysuckle, 0.6g of radix isatidis and 65g of carbonate buffer solution into distilled water, and uniformly stirring and mixing to obtain the compound polypeptide mouthwash.
Example 4
A preparation method of a compound polypeptide mouthwash comprises the following steps:
adding 8g of biological antibacterial peptide, 0.7g of melittin, 0.8g of casein phosphopeptide, 0.9g of biological lysozyme, 1.5g of chitosan, 0.6g of natamycin, 1.5g of honeysuckle, 1.8g of radix isatidis and 300g of carbonate buffer solution into distilled water, and uniformly stirring and mixing to obtain the compound polypeptide mouthwash.
Example 5
A preparation method of a compound polypeptide mouthwash comprises the following steps:
adding 10g of biological antibacterial peptide, 1g of melittin, 1g of casein phosphopeptide, 1g of biological lysozyme, 2g of chitosan, 1g of natamycin, 2g of honeysuckle, 2g of radix isatidis and 900g of carbonate buffer solution into distilled water, and uniformly stirring and mixing to obtain the compound polypeptide mouthwash.
Comparative example 1
A preparation method of a compound polypeptide mouthwash comprises the following steps:
adding 0.01g of melittin, 0.2g of casein phosphopeptide, 0.2g of biological lysozyme, 0.2g of chitosan, 0.1g of natamycin, 0.1g of honeysuckle, 0.1g of radix isatidis and 5g of carbonate buffer solution into distilled water, and uniformly stirring and mixing to obtain the compound polypeptide mouthwash.
Comparative example 2
A preparation method of a compound polypeptide mouthwash comprises the following steps:
adding 0.1g of biological antibacterial peptide, 0.2g of casein phosphopeptide, 0.2g of biological lysozyme, 0.2g of chitosan, 0.1g of natamycin, 0.1g of honeysuckle, 0.1g of radix isatidis and 5g of carbonate buffer solution into distilled water, and uniformly stirring and mixing to obtain the compound polypeptide mouthwash.
Comparative example 3
A preparation method of a compound polypeptide mouthwash comprises the following steps:
adding 0.2g of casein phosphopeptide, 0.2g of biological lysozyme, 0.2g of chitosan, 0.1g of natamycin, 0.1g of honeysuckle, 0.1g of radix isatidis and 5g of carbonate buffer solution into distilled water, and uniformly stirring and mixing to obtain the compound polypeptide mouthwash.
The mouthwashes obtained in examples 1 to 5 and comparative examples 1 to 3 were compared experimentally, as follows:
1. sterilization test
The mouthwash obtained in examples 1-5 and comparative examples 1-3 is subjected to WS/T650-2019 & lt evaluation method for antibacterial and bacteriostatic effects & gt, and the bactericidal level of the antibacterial mouthwash prepared by the invention is detected by a suspension quantitative method so as to measure the bactericidal capacity of the antibacterial mouthwash. The bacteria to be detected are three types of bacteria, namely staphylococcus aureus (ATCC6538), escherichia coli (ATCC8099) and candida albicans (ATCC10231), the detection concentration is 1:1, the action time is 1min, and the result is shown in the following table 1.
The mouthwash obtained in examples 1-5 and comparative examples 1-3 is used for measuring the average bactericidal rate of helicobacter pylori and lactobacillus plantarum according to the WS/T650-2019 'evaluation method of antibacterial and bacteriostatic effect', wherein the sample concentration is 100%, the action time is 1min when measured by a suspension drop method, and the test results are shown in Table 1.
TABLE 1 results of the bactericidal Property test of the antibacterial mouthwashes of examples and comparative examples
As can be seen from Table 1, the mouth washes of examples 1-5 all have good bactericidal effects, and the bactericidal rates for three common bacteria, namely staphylococcus aureus, escherichia coli and candida albicans, all reach more than 97%; meanwhile, the mouthwash can well kill helicobacter pylori, the sterilization rate reaches more than 98 percent, beneficial flora such as lactobacillus plantarum in the oral cavity is not damaged, and the oral cavity health is facilitated.
Comparing example 1 with comparative example 1, comparative example 2 and comparative example 3, it can be seen that when the biological antibacterial peptide, the melittin and the biological lysomycete are added simultaneously, the sterilization rate reaches 97%; when the biological antibacterial peptide or the melittin is lacked, the sterilization rates of the two are not very different and are about 85 percent; when the biological antibacterial peptide and the melittin are lacked, the sterilization rate is reduced to 76 percent, which shows that the biological antibacterial peptide, the melittin and the biological lysomycete not only have the sterilization function, but also can promote the sterilization effect when being mixed for use, and have the synergistic effect.
2. Acute oral toxicity test
The mouth wash of example 1 was tested for acute oral toxicity in mice according to the acute oral toxicity test in the Disinfection Specification (2002 edition), by the following specific methods: test subjects: 20 KM mice are selected, and the number of the KM mice is half that of the KM mice; the preparation method of the test substance comprises the following steps: take preparation of 10mL as an example: 5000mg of the mouthwash in example 1 is taken and is made into a volume of 10mL by using distilled water, and the concentration is 500 mg/mL. Test sample administration methods: according to the test method of acute oral toxicity in the 2002 edition of disinfection technical Specification: after 3-5 days of quarantine confirmation after the purchase of the mice, the mice are fasted overnight before the administration of the test substances, drinking water is not limited, and the test samples are usedThe preparation was administered to animals once a day by oral gavage at a rate of 0.1mL/10g body weight. After the test sample solution is administered, the animals are continuously observed for 14 days, the animal status and death condition are recorded, the number of dead animals and the number of surviving animals within 14 days after the test sample solution is administered are respectively counted, and LD is calculated50(median lethal dose). LD obtained by calculation50The toxicity of the test substance was evaluated according to the following criteria. LD50The people with the weight more than 5000mg/kg are actually nontoxic; LD50The patients with 501 mg/kg-5000 mg/kg body weight belong to low toxicity; LD50The body weight of 51 mg/kg-500 mg/kg belongs to moderate toxicity; LD50The patients with 1 mg/kg-50 mg/kg body weight belong to high toxicity; LD50Those with the weight less than 1mg/kg are extremely toxic. The results of the experiment are shown in table 2.
TABLE 2 acute oral toxicity test results
As can be seen from Table 2, the mouthwash of the present invention is safe and practically nontoxic according to the Standard for grading acute toxicity of Disinfection technical Specification (2002 edition).
The mouthwashes prepared in examples 2-5 were subjected to the same acute oral toxicity test as in example 1, and the test results were the same as in example 1, indicating that the mouthwashes prepared in examples 2-5 were practically nontoxic.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (5)
1. A compound polypeptide mouthwash is characterized in that: the mouthwash comprises natural biological components, traditional Chinese medicine components and a pH buffer solution, wherein the natural biological components comprise biological antibacterial peptide, melittin, casein phosphopeptide, biological lysozyme, chitosan and natamycin, and the traditional Chinese medicine components comprise honeysuckle and isatis root.
2. The complex polypeptide mouthwash of claim 1, wherein: the natural biological component comprises the following components in parts by weight: 0.1-10 parts of biological antibacterial peptide, 0.01-1 part of melittin, 0.2-1 part of casein phosphopeptide, 0.2-1 part of biological lysozyme, 0.2-2 parts of chitosan and 0.1-1 part of natamycin.
3. The complex polypeptide mouthwash of claim 1, wherein: the traditional Chinese medicine comprises the following components in parts by weight: 0.1-2 parts of honeysuckle and 0.1-2 parts of isatis root.
4. The complex polypeptide mouthwash of claim 1, wherein: the pH buffer solution is phosphate buffer solution or carbonate buffer solution, and the weight percentage of the pH buffer solution in the mouthwash is 80-98.99%.
5. A method for preparing the compound polypeptide mouth wash as claimed in any one of claims 1 to 4, which comprises the following steps: adding the biological antibacterial peptide, melittin, casein phosphopeptide, biological lysozyme, chitosan, natamycin, honeysuckle, radix isatidis and pH buffer solution into distilled water, and uniformly stirring and mixing to obtain the compound polypeptide mouthwash.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113662897A (en) * | 2021-10-08 | 2021-11-19 | 利川市常大产业技术研究院有限公司 | Mouthwash and preparation method thereof |
| CN114028546A (en) * | 2021-12-02 | 2022-02-11 | 北京洗得宝消毒制品有限公司 | Composite polypeptide sterilization preparation and preparation method and application thereof |
| CN114146013A (en) * | 2021-12-02 | 2022-03-08 | 北京洗得宝消毒制品有限公司 | Mouthwash for killing helicobacter pylori, preparation method and portable packaging bag |
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| CN113662897A (en) * | 2021-10-08 | 2021-11-19 | 利川市常大产业技术研究院有限公司 | Mouthwash and preparation method thereof |
| CN113662897B (en) * | 2021-10-08 | 2023-02-28 | 利川市常大产业技术研究院有限公司 | Mouthwash and preparation method thereof |
| CN114028546A (en) * | 2021-12-02 | 2022-02-11 | 北京洗得宝消毒制品有限公司 | Composite polypeptide sterilization preparation and preparation method and application thereof |
| CN114146013A (en) * | 2021-12-02 | 2022-03-08 | 北京洗得宝消毒制品有限公司 | Mouthwash for killing helicobacter pylori, preparation method and portable packaging bag |
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