CN113430197B - 促进砧木根系发育及抗旱的可移动miRNA及鉴定方法 - Google Patents
促进砧木根系发育及抗旱的可移动miRNA及鉴定方法 Download PDFInfo
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CN114752696B (zh) * | 2022-02-21 | 2023-03-24 | 中国农业大学 | 一种检测接穗评价砧木优劣的芯片及其制备方法和应用 |
CN115011610B (zh) * | 2022-06-17 | 2023-06-23 | 西北农林科技大学 | MdTCP17和MdWOX11互作调控MdLBD29基因表达和不定根发生的应用 |
CN116891854A (zh) * | 2023-04-13 | 2023-10-17 | 西北农林科技大学 | 一种苹果mRNA及其在提高苹果抗逆性中的应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101203611A (zh) * | 2005-04-19 | 2008-06-18 | 巴斯福植物科学有限公司 | 控制基因表达的改良方法 |
CN102149821A (zh) * | 2008-07-01 | 2011-08-10 | 孟山都技术公司 | 用于调控靶基因表达的重组dna构建体和方法 |
CN105985954A (zh) * | 2015-01-27 | 2016-10-05 | 华中农业大学 | 水稻miR160b基因在调控分蘖角度中的应用 |
CN109072250A (zh) * | 2016-04-14 | 2018-12-21 | 瑞克斯旺种苗集团公司 | 改变mRNA的细胞间迁移性的方法 |
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US8624084B2 (en) * | 2007-03-16 | 2014-01-07 | Gloria Coruzzi | Pericycle-specific expression of microrna 167 in plants |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101203611A (zh) * | 2005-04-19 | 2008-06-18 | 巴斯福植物科学有限公司 | 控制基因表达的改良方法 |
CN102149821A (zh) * | 2008-07-01 | 2011-08-10 | 孟山都技术公司 | 用于调控靶基因表达的重组dna构建体和方法 |
CN105985954A (zh) * | 2015-01-27 | 2016-10-05 | 华中农业大学 | 水稻miR160b基因在调控分蘖角度中的应用 |
CN109072250A (zh) * | 2016-04-14 | 2018-12-21 | 瑞克斯旺种苗集团公司 | 改变mRNA的细胞间迁移性的方法 |
Non-Patent Citations (4)
Title |
---|
The interaction between miR160 and miR165/166 in the control of leaf development and drought tolerance in Arabidopsis;TianxiaoYang et al.;《Scientific reports》;20190226;第9卷(第2832期);第1-13页 * |
The positive feedback regulatory loop of miR160-Auxin Response Factor 17-HYPONASTIC LEAVES 1 mediates drought tolerance in apple trees;Xiaoxia Shen et al.;《Plant Physiology》;20211206;第188卷;第1686-1708页 * |
植物微RNA的生物学功能及其在砧穗互作中的作用研究进展;徐媛媛 等;《中国南方果树》;20170520;第46卷(第3期);第172-178页 * |
苹果砧木不定根发生相关非编码RNA鉴定与作用机制研究;孟媛;《中国优秀博硕士学位论文全文数据库(硕士) 农业科技辑》;20190815(第8期);D048-95 * |
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