CN113348250A - 核酸合成装置和纯化装置、其用途及核酸合成方法和纯化方法 - Google Patents
核酸合成装置和纯化装置、其用途及核酸合成方法和纯化方法 Download PDFInfo
- Publication number
- CN113348250A CN113348250A CN201880099717.2A CN201880099717A CN113348250A CN 113348250 A CN113348250 A CN 113348250A CN 201880099717 A CN201880099717 A CN 201880099717A CN 113348250 A CN113348250 A CN 113348250A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- cpg
- purified
- synthesis
- purification apparatus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001668 nucleic acid synthesis Methods 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 57
- 238000001821 nucleic acid purification Methods 0.000 title claims description 80
- 239000005289 controlled pore glass Substances 0.000 claims abstract description 119
- 230000002194 synthesizing effect Effects 0.000 claims abstract description 39
- 239000007790 solid phase Substances 0.000 claims abstract description 38
- 238000000746 purification Methods 0.000 claims abstract description 35
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims abstract description 29
- 239000000178 monomer Substances 0.000 claims abstract description 12
- 239000002773 nucleotide Substances 0.000 claims abstract description 11
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 11
- 229920000642 polymer Polymers 0.000 claims abstract description 11
- 150000008300 phosphoramidites Chemical class 0.000 claims abstract description 8
- 150000007523 nucleic acids Chemical class 0.000 claims description 129
- 108020004707 nucleic acids Proteins 0.000 claims description 126
- 102000039446 nucleic acids Human genes 0.000 claims description 126
- 108091034117 Oligonucleotide Proteins 0.000 claims description 55
- -1 polyethylene Polymers 0.000 claims description 37
- 239000000047 product Substances 0.000 claims description 36
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 35
- 239000011148 porous material Substances 0.000 claims description 31
- 239000004698 Polyethylene Substances 0.000 claims description 27
- 229920000573 polyethylene Polymers 0.000 claims description 26
- 239000004699 Ultra-high molecular weight polyethylene Substances 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 20
- 239000002952 polymeric resin Substances 0.000 claims description 20
- 229920001169 thermoplastic Polymers 0.000 claims description 20
- 229920000785 ultra high molecular weight polyethylene Polymers 0.000 claims description 20
- 229920001903 high density polyethylene Polymers 0.000 claims description 19
- 239000004700 high-density polyethylene Substances 0.000 claims description 19
- 238000003786 synthesis reaction Methods 0.000 claims description 19
- 230000015572 biosynthetic process Effects 0.000 claims description 18
- 239000007787 solid Substances 0.000 claims description 16
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 12
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
- 238000005245 sintering Methods 0.000 claims description 12
- 230000000295 complement effect Effects 0.000 claims description 11
- 239000002904 solvent Substances 0.000 claims description 10
- 238000007098 aminolysis reaction Methods 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 8
- QXJJQWWVWRCVQT-UHFFFAOYSA-K calcium;sodium;phosphate Chemical compound [Na+].[Ca+2].[O-]P([O-])([O-])=O QXJJQWWVWRCVQT-UHFFFAOYSA-K 0.000 claims description 8
- 238000010511 deprotection reaction Methods 0.000 claims description 8
- 239000012071 phase Substances 0.000 claims description 8
- 239000004094 surface-active agent Substances 0.000 claims description 8
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 8
- 239000012498 ultrapure water Substances 0.000 claims description 8
- 230000002209 hydrophobic effect Effects 0.000 claims description 7
- 238000011534 incubation Methods 0.000 claims description 7
- 239000007795 chemical reaction product Substances 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 4
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 4
- 239000003945 anionic surfactant Substances 0.000 claims description 4
- 239000003125 aqueous solvent Substances 0.000 claims description 4
- 229960002233 benzalkonium bromide Drugs 0.000 claims description 4
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 4
- 239000003093 cationic surfactant Substances 0.000 claims description 4
- 238000003776 cleavage reaction Methods 0.000 claims description 4
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 4
- 230000002255 enzymatic effect Effects 0.000 claims description 4
- 239000000194 fatty acid Substances 0.000 claims description 4
- 229930195729 fatty acid Natural products 0.000 claims description 4
- 239000000787 lecithin Substances 0.000 claims description 4
- 229940067606 lecithin Drugs 0.000 claims description 4
- 235000010445 lecithin Nutrition 0.000 claims description 4
- 239000002736 nonionic surfactant Substances 0.000 claims description 4
- 229920000136 polysorbate Polymers 0.000 claims description 4
- 229950008882 polysorbate Drugs 0.000 claims description 4
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 4
- 150000003856 quaternary ammonium compounds Chemical class 0.000 claims description 4
- 230000007017 scission Effects 0.000 claims description 4
- 229940100515 sorbitan Drugs 0.000 claims description 4
- 239000002888 zwitterionic surfactant Substances 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 238000009736 wetting Methods 0.000 claims description 3
- 229940083575 sodium dodecyl sulfate Drugs 0.000 claims description 2
- 239000003155 DNA primer Substances 0.000 abstract description 7
- 238000001976 enzyme digestion Methods 0.000 abstract description 3
- 108020004414 DNA Proteins 0.000 description 63
- 239000002245 particle Substances 0.000 description 23
- 238000003752 polymerase chain reaction Methods 0.000 description 23
- 239000000243 solution Substances 0.000 description 17
- 238000006243 chemical reaction Methods 0.000 description 15
- 239000003153 chemical reaction reagent Substances 0.000 description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 12
- 239000004743 Polypropylene Substances 0.000 description 9
- 239000012634 fragment Substances 0.000 description 9
- 229920001155 polypropylene Polymers 0.000 description 9
- 230000006820 DNA synthesis Effects 0.000 description 8
- 238000011084 recovery Methods 0.000 description 8
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 7
- 238000001746 injection moulding Methods 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 239000011324 bead Substances 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
- 239000012535 impurity Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000000741 silica gel Substances 0.000 description 5
- 229910002027 silica gel Inorganic materials 0.000 description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 239000000969 carrier Substances 0.000 description 4
- 125000003636 chemical group Chemical group 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 239000011543 agarose gel Substances 0.000 description 3
- 230000001788 irregular Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000013615 primer Substances 0.000 description 3
- 229910052814 silicon oxide Inorganic materials 0.000 description 3
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000005915 ammonolysis reaction Methods 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 230000003196 chaotropic effect Effects 0.000 description 2
- 231100000481 chemical toxicant Toxicity 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000004005 microsphere Substances 0.000 description 2
- 231100000956 nontoxicity Toxicity 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 125000006239 protecting group Chemical group 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 239000008279 sol Substances 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- 125000001731 2-cyanoethyl group Chemical group [H]C([H])(*)C([H])([H])C#N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000006482 condensation reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000007793 ph indicator Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/40—Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00759—Purification of compounds synthesised
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
Abstract
一种核酸合成装置和纯化装置、其用途及核酸合成方法和纯化方法，该核酸合成装置包括固相载体，该固相载体包括可控孔径玻璃球(CPG)，该CPG是未被修饰的裸CPG，其表面具有羟基，羟基用作核酸合成的起始点，用于通过共价键合连接亚磷酰胺保护的核苷酸单体或多聚体以进行核酸合成。本发明的核酸合成装置，不仅可以用于寡核苷酸引物的合成，还可以利用固定在CPG上的寡核苷酸引物进行酶切、PCR产物的纯化等，具有结构简单、体积小、重量轻、效率高、成本低、功能多样的优点。
Description
PCT国内申请，说明书已公开。
Claims (47)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2018/121092 WO2020118655A1 (zh) | 2018-12-14 | 2018-12-14 | 核酸合成装置和纯化装置、其用途及核酸合成方法和纯化方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113348250A true CN113348250A (zh) | 2021-09-03 |
Family
ID=71075557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201880099717.2A Pending CN113348250A (zh) | 2018-12-14 | 2018-12-14 | 核酸合成装置和纯化装置、其用途及核酸合成方法和纯化方法 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US20220025354A1 (zh) |
| CN (1) | CN113348250A (zh) |
| WO (1) | WO2020118655A1 (zh) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114653415A (zh) * | 2022-03-31 | 2022-06-24 | 深圳市曙芯生物科技有限公司 | 脱氧核糖核酸合成芯片及其合成方法 |
| CN114931925A (zh) * | 2022-05-24 | 2022-08-23 | 通用生物(安徽)股份有限公司 | 一种高分子聚合物制作的Frits反应载体及其应用 |
| CN116143447A (zh) * | 2022-12-22 | 2023-05-23 | 河北迪纳兴科生物科技有限公司 | Dna合成柱及其制备方法 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115646382B (zh) * | 2022-09-09 | 2023-08-04 | 北京擎科生物科技股份有限公司 | 一种全自动核酸纯化洗脱仪 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103228798A (zh) * | 2010-09-24 | 2013-07-31 | 斯坦福大学托管董事会 | 使用固定的引物直接捕获、扩增及测序靶标dna |
| CN204151332U (zh) * | 2014-09-16 | 2015-02-11 | 深圳逗点生物技术有限公司 | 一种dna合成柱 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5904848A (en) * | 1996-02-21 | 1999-05-18 | Cpg, Inc. | Controlled pore glass-synthetic resin membrane |
| AU2001275995A1 (en) * | 2000-07-31 | 2002-02-13 | Applera Corporation | Apparatus and method for specific release of captured extension products |
| CN106048736B (zh) * | 2015-04-14 | 2019-09-03 | 成都先导药物开发股份有限公司 | 一种固相合成dna编码化合物库的方法 |
| WO2016193282A1 (en) * | 2015-06-01 | 2016-12-08 | Qiagen Gmbh | Electrophoresis assisted method for purifying a target nucleic acid using a delayed elution approach |
| CN107383246A (zh) * | 2017-08-28 | 2017-11-24 | 盐城师范学院 | 一种聚苯乙烯基质核酸固相有机合成载体及其制备方法 |
| CN207483713U (zh) * | 2017-09-26 | 2018-06-12 | 深圳市百迈生命科学有限公司 | 一种创新的微量dna合成用96孔板cpg载体 |
-
2018
- 2018-12-14 US US17/311,697 patent/US20220025354A1/en active Pending
- 2018-12-14 WO PCT/CN2018/121092 patent/WO2020118655A1/zh active Application Filing
- 2018-12-14 CN CN201880099717.2A patent/CN113348250A/zh active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103228798A (zh) * | 2010-09-24 | 2013-07-31 | 斯坦福大学托管董事会 | 使用固定的引物直接捕获、扩增及测序靶标dna |
| CN204151332U (zh) * | 2014-09-16 | 2015-02-11 | 深圳逗点生物技术有限公司 | 一种dna合成柱 |
Non-Patent Citations (3)
| Title |
|---|
| FARRE ET AL.: "Automated oligonucleotide solid-phase synthesis on nanosized silica particles using nano-on-micro assembled particle supports", 《LANGMUIR》, vol. 26, no. 7, pages 4941 - 4950, XP055767353, DOI: 10.1021/la903572q * |
| GUZAEV ET AL.: "Attachment of nucleosides and other linkers to solid-phase supports for oligonucleotide synthesis", 《CURRENT PROTOCOLS IN NUCLEIC ACID CHEMISTRY》, pages 1 - 3 * |
| GUZAEV ET AL.: "Solid-phase supports for oligonucleotide synthesis", 《CURRENT PROTOCOLS IN NUCLEIC ACID CHEMISTRY》, pages 1 - 3 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114653415A (zh) * | 2022-03-31 | 2022-06-24 | 深圳市曙芯生物科技有限公司 | 脱氧核糖核酸合成芯片及其合成方法 |
| CN114931925A (zh) * | 2022-05-24 | 2022-08-23 | 通用生物(安徽)股份有限公司 | 一种高分子聚合物制作的Frits反应载体及其应用 |
| CN116143447A (zh) * | 2022-12-22 | 2023-05-23 | 河北迪纳兴科生物科技有限公司 | Dna合成柱及其制备方法 |
| CN116143447B (zh) * | 2022-12-22 | 2024-01-30 | 河北迪纳兴科生物科技有限公司 | Dna合成柱及其制备方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2020118655A1 (zh) | 2020-06-18 |
| US20220025354A1 (en) | 2022-01-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113348250A (zh) | 核酸合成装置和纯化装置、其用途及核酸合成方法和纯化方法 | |
| US6617105B1 (en) | Solid-phase nucleic acid isolation | |
| Ali et al. | Current nucleic acid extraction methods and their implications to point‐of‐care diagnostics | |
| US9624252B2 (en) | Selective nucleic acid fragment recovery | |
| EP1147226B1 (en) | Materials and methods for the purification of polyelectrolytes | |
| ES2233560T3 (es) | Aislamiento de acidos nucleicos. | |
| AU2001260507C1 (en) | Nucleic acid isolation | |
| US11643646B2 (en) | Method for isolating and purifying nucleic acids using a solid-liquid phase system | |
| EP3129498B1 (en) | Nucleic acid purification method | |
| JP2008054660A (ja) | 核酸を単離するためのろ過装置 | |
| JP2002543980A (ja) | 混合床固相及び核酸の単離におけるその使用 | |
| JP5867402B2 (ja) | ｓｍａｌｌＲＮＡの取得方法 | |
| EP1316606A1 (en) | Method for separation and purification of nucleic acid and unit for separation and purification of nucleic acid | |
| US5817798A (en) | Rapid RNA isolation procedure in the presence of a transition metal ion | |
| CA2428532C (en) | Lysate clearance and nucleic acid isolation using silanized silica matrices | |
| CN108473983B (zh) | 使用单一洗涤和洗脱缓冲溶液的核酸纯化系统 | |
| Nasir et al. | Current nucleic acid extraction methods and their implications to point-of-care diagnostics | |
| US20110313145A1 (en) | Isolation of rna | |
| US8975017B2 (en) | Process for concentrating nucleic acid molecules | |
| WO2021100801A1 (ja) | 核酸の分離方法、検出方法、核酸精製カラム及びその製造方法 | |
| CN115141719A (zh) | 一种无阻碍吸入式动态快速提取核酸的方法及装置 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |