CN113336601A - Method for preparing biocontrol bacterial manure by bacteria and method for eliminating continuous cropping obstacles of melons - Google Patents

Method for preparing biocontrol bacterial manure by bacteria and method for eliminating continuous cropping obstacles of melons Download PDF

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Publication number
CN113336601A
CN113336601A CN202110823099.5A CN202110823099A CN113336601A CN 113336601 A CN113336601 A CN 113336601A CN 202110823099 A CN202110823099 A CN 202110823099A CN 113336601 A CN113336601 A CN 113336601A
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bacteria
soil
biocontrol
melons
preparing
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CN202110823099.5A
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Inventor
农倩
廖仕同
龙艳艳
李长宁
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Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
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Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C1/00—Ammonium nitrate fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Fertilizers (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a method for preparing biocontrol bacterial manure by bacteria and a method for eliminating continuous cropping obstacles of melons, which comprises the steps of taking bacillus subtilis separated from a vineyard as a strain, and separating and identifying the strain; sterilizing the carrier matrix; sterilizing semen glycines powder, ammonium nitrate, sucrose, magnesium sulfate, calcium sulfate, and manganese sulfate. The biological control bacterial manure produced by rice bran, wheat bran or corn broken particles is applied to the melon continuous cropping soil, so that biological control bacteria can be brought in, a large amount of organic matters can be brought in the soil, the raw materials are easy to obtain, the price is low, the operation is simple, most of pathogenic bacteria in the soil can be killed by applying lime nitrogen and quicklime to the soil, the occurrence of soil-borne diseases is effectively reduced, an amino acid foliar fertilizer is sprayed, the crop growth is promoted, the quality of crops is improved, and the quality of the melons can be improved by spraying the biological control bacterial manure in the growth period of the melons.

Description

Method for preparing biocontrol bacterial manure by bacteria and method for eliminating continuous cropping obstacles of melons
Technical Field
The invention relates to the technical field of planting industry, in particular to a method for preparing biocontrol bacterial manure by bacteria and a method for eliminating continuous cropping obstacles of melons.
Background
The continuous cropping obstacle is the phenomenon that after the same kind or same family crops are continuously planted in the same plot for many years, even if normal cultivation management measures are adopted, the growth vigor of plants is weakened, the plant diseases and insect pests are aggravated, and the yield and the quality are reduced. In ancient China, some records about disease aggravation after continuous cropping of crops are called continuous cropping diseases, and European and American countries call soil-borne diseases, continuous cropping obstacles and replanting diseases. The successive cropping obstacles are characterized by: weak seedling growth, poor root system development, susceptibility to diseases, reduced plant stress resistance and the like.
Soil-borne diseases are treated by measures such as root irrigation and the like to eliminate soil fungi, but when the pesticide is applied to kill the soil fungi, other microorganisms in the soil are also killed; the long-term production of a single crop variety finally causes the deterioration of the physical and chemical properties of soil, which is not beneficial to the growth of crops; and the application of agriculture and chemical fertilizers can promote the growth of crops, but can not eliminate the self-toxic substances generated by the root systems of plants in the soil.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a method for preparing biocontrol bacterial manure by bacteria and a method for eliminating continuous cropping obstacles of melons.
In order to solve the technical problems, the invention provides the following technical scheme:
the invention relates to a method for preparing a biocontrol bacterial fertilizer by bacteria, which comprises the following steps:
a. taking bacillus subtilis separated in a vineyard as a strain, and separating and identifying the strain;
b. sterilizing the carrier matrix;
c. b, adding sterilized soybean meal, ammonium nitrate, cane sugar, magnesium sulfate, calcium sulfate and manganese sulfate into the carrier matrix in the step b, adding sterile water and seed liquid of biocontrol bacteria, and fully and uniformly stirring to obtain a fermentation raw material;
d. and c, fermenting the fermentation raw material obtained in the step c to ensure that the number of biocontrol bacteria in the substrate carrier reaches 1100CFU/gDW carrier substrate.
As a preferable technical scheme of the invention, the carrier matrix in the step b is rice bran, wheat bran or corn material, and in the step b, the wet heat sterilization is carried out under the conditions of 80-100Kpa and 130 ℃ at 100 ℃.
As a preferred technical scheme of the invention, in the step c, the dosage of the soybean meal, the ammonium nitrate, the sucrose, the magnesium sulfate, the calcium sulfate and the manganese sulfate is 1% -3%, 2% -4%, 0.2% -0.5%, 0.1% -0.2%, 0.02% -0.06%, 0.02% -0.05% of the weight of the fermentation substrate in sequence, the dosage of the sterile water is 50% -65% of the weight of the carrier substrate, the dosage of the biocontrol bacteria seed solution is 1% -1.2% of the weight of the carrier substrate, the total number of bacterial colonies is 4 × 110CFU/mL, and the biocontrol bacteria seed solution is fresh bacterial liquid with the storage time of not more than 36-48 h.
In a preferred embodiment of the present invention, the fermentation of the fermentation material in step d is performed in a thermal insulation device, wherein the initial temperature is set to 30-38 ℃, the fermentation time is 20-24 hours, and the ventilation rate in the high temperature period is adjusted to 0.13-0.16m3/(m3min), and ensuring that the temperature of the carrier matrix is maintained to be 38-46 ℃ for fermentation for 48 h.
The invention provides a second technical scheme as follows:
the invention also provides a method for eliminating continuous cropping obstacles of melons by using the biocontrol bacterial manure prepared by bacteria, which specifically comprises the following steps:
a. the method comprises the steps of taking bacillus subtilis separated from a vineyard as a strain, preparing a biocontrol bacterial fertilizer, and uniformly spreading the prepared biocontrol bacterial fertilizer to a melon field according to 138 kg/mu of 134-year-old seeds one month before melon seedling transplantation;
b. spreading lime nitrogen and quicklime into the melon planting soil during the fallow period, turning over, leveling, sealing and watering, covering the soil with a white film after watering to enable the planting area temperature to reach more than 55 ℃, and sealing the greenhouse for 26-35 days;
c. b, after the step b is finished, removing the plastic film, performing ventilation treatment, then performing ploughing, performing ventilation treatment, spraying bio-control bacterial fertilizer and bio-fertilizer, performing rotary tillage, uniformly mixing, ridging, covering a black film, and planting the cultivated sweet melon seedlings in soil; and in the growth process of the melons, the amino acid foliar fertilizer is sprayed to the melons once every 9 to 14 days.
As a preferred technical scheme of the invention, in the steps a and c, the muskmelon field is selected from nutrient soil without pathogenic bacteria, and 1500-.
As a preferred technical scheme of the present invention, the spreading amount of the lime nitrogen and the quicklime in the step b is respectively: lime nitrogen 50-60 kg/667 m250-60 kg of quicklime/667 m2。
Compared with the prior art, the invention has the following beneficial effects:
the invention can effectively inhibit the growth of soil fungi through biocontrol bacteria, promote the growth of soil bacteria, balance the balance of soil microbial communities, has the function of decomposing coumaric acid which is the main component of melon root secretion, can reduce or even eliminate phenolic acid substances in continuous cropping soil, adopts biocontrol bacterial manure produced by rice bran, wheat bran or corn crushed particles, is applied to the melon continuous cropping soil, can bring biocontrol bacteria into the soil and bring a large amount of organic matters into the soil, can improve the physical and chemical properties of the soil and the health state of the continuous cropping soil, has simple process, easily obtained raw materials, low price and simple operation, is stable and safe in bacterial manure products, can kill most pathogenic bacteria in the soil by applying lime nitrogen and quicklime into the soil, effectively reduces the occurrence of soil-borne diseases, sprays amino acid foliar fertilizer, promotes the growth of crops and improves the quality of the crops, and the spraying is carried out in the melon growth period, so that the quality of the melon can be improved, and the yield of the melon can be increased.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention.
Example 1
The invention provides a method for preparing a biocontrol bacterial fertilizer by bacteria, which comprises the following steps:
a. taking bacillus subtilis separated in a vineyard as a strain, and separating and identifying the strain;
b. performing moist heat sterilization on the rice bran at the temperature of 100 ℃ and at the pressure of 80 Kpa;
c. b, adding sterilized 1% of bean flour, 2% of ammonium nitrate, 0.2% of cane sugar, 0.1% of magnesium sulfate, 0.02% of calcium sulfate and 0.02% of manganese sulfate into the carrier matrix in the step b, adding sterile water accounting for 50% of the weight of the carrier matrix and seed liquid of biocontrol bacteria accounting for 1% of the weight of the carrier matrix, wherein the seed liquid of the biocontrol bacteria is fresh bacterial liquid which is stored for no more than 36-48 hours, and fully and uniformly stirring to obtain a fermentation raw material;
d. c, fermenting the fermentation material obtained in the step c in a heat preservation device, setting the initial temperature to be 32 ℃, fermenting for 22h, and adjusting the ventilation quantity in the high temperature period to be 0.14m3/(m3min), the temperature of the carrier matrix is maintained to be 40 ℃, fermentation is carried out for 48 hours, and the number of biocontrol bacteria in the matrix carrier is ensured to reach 1100CFU/gDW carrier matrix.
The invention provides a method for eliminating continuous cropping obstacles of melons by using biocontrol bacterial manure prepared by bacteria, which specifically comprises the following steps:
a. the method comprises the following steps of taking bacillus subtilis separated from a vineyard as a strain, preparing biocontrol bacterial manure, and uniformly spreading the prepared biocontrol bacterial manure to melon fields according to 136 kg/mu one month before melon seedlings are transplanted;
b. during the fallow period, 50 kg/667 m of soil for planting the melons is scattered2Lime nitrogen and 50 kg/667 m2Then turning over the quicklime, leveling, sealing and watering, wherein the sealing temperature is maintained at 550 ℃ for 25 days, after watering, covering the soil with a white film to ensure that the temperature of a planting area reaches above 55 ℃, and sealing the greenhouse for 26 days;
c. after the step b is finished, removing the plastic film, performing ventilation treatment, then ploughing, performing ventilation treatment, and spreading 134 kg/mu of biocontrol bacterial manure and 400 kg/667 m2The biological fertilizer is subjected to rotary tillage, uniform mixing, ridging and coveringBlack film, namely planting the cultivated sweet melon seedlings in soil; and in the growth process of the melons, the amino acid foliar fertilizer is sprayed to the melons once every 12 days.
Example 2
The invention provides a method for preparing a biocontrol bacterial fertilizer by bacteria, which comprises the following steps:
a. taking bacillus subtilis separated in a vineyard as a strain, and separating and identifying the strain;
b. performing moist heat sterilization on the wheat bran at 90Kpa and 110 ℃;
c. b, adding 2% of sterilized bean flour, 3% of ammonium nitrate, 0.3% of sucrose, 0.3% of magnesium sulfate, 0.04% of calcium sulfate and 0.03% of manganese sulfate into the carrier matrix in the step b, adding 55% of sterile water and 1.1% of biocontrol bacteria seed liquid into the carrier matrix, wherein the biocontrol bacteria seed liquid is fresh bacterial liquid which is stored for no more than 36-48 hours, and fully and uniformly stirring to obtain a fermentation raw material;
d. c, fermenting the fermentation material obtained in the step c in a heat preservation device, wherein the initial temperature is 33 ℃, the fermentation time is 24 hours, and the ventilation in the high temperature period is adjusted to be 0.15m3/(m3min), the temperature of the carrier matrix is maintained to 42 ℃, and fermentation is carried out for 48 hours, so that the number of biocontrol bacteria in the matrix carrier is ensured to reach 1100CFU/gDW carrier matrix.
The invention provides a method for eliminating continuous cropping obstacles of melons by using biocontrol bacterial manure prepared by bacteria, which specifically comprises the following steps:
a. the method comprises the following steps of taking bacillus subtilis separated from a vineyard as a strain, preparing biocontrol bacterial manure, and uniformly spreading the prepared biocontrol bacterial manure to melon fields according to 136 kg/mu one month before melon seedlings are transplanted;
b. during the fallow period, 55 kg/667 m of soil for planting the melons is scattered2Lime nitrogen and 55 kg/667 m2Then turning over the quicklime, leveling, sealing and watering, keeping the sealing temperature at 450 ℃ for 25 days, covering the watered quicklime on the soil by using a white film to ensure that the temperature of a planting area reaches above 50 ℃, and sealing the greenhouse for 30 days;
c. after step b is finishedRemoving the plastic film, ventilating, ploughing, and spreading 134 kg/mu biocontrol bacterial fertilizer and 420 kg/667 m2Carrying out rotary tillage and uniform mixing on the biological fertilizer, ridging, covering a black film, and planting the cultured sweet melon seedlings in soil; and in the growth process of the melons, the amino acid foliar fertilizer is sprayed to the melons once every 10 days.
Example 3
The invention provides a method for preparing a biocontrol bacterial fertilizer by bacteria, which comprises the following steps:
a. taking bacillus subtilis separated in a vineyard as a strain, and separating and identifying the strain;
b. performing moist heat sterilization on the corn material at 100Kpa and 130 ℃;
c. adding 3% of sterilized bean flour, 4% of ammonium nitrate, 0.5% of cane sugar, 0.1% of magnesium sulfate, 0.06% of calcium sulfate and 0.05% of manganese sulfate into the carrier matrix in the step b, adding 65% of sterile water and 1.2% of biocontrol bacteria seed liquid into the carrier matrix, wherein the biocontrol bacteria seed liquid is fresh bacterial liquid which is stored for no more than 36-48 hours, and fully and uniformly stirring to obtain a fermentation raw material;
d. c, fermenting the fermentation material obtained in the step c in a heat preservation device, wherein the initial temperature is 38 ℃, the fermentation time is 24 hours, and the ventilation in the high temperature period is adjusted to be 0.16m3/(m3min), the temperature of the carrier matrix is maintained to 46 ℃, and fermentation is carried out for 48 hours, so that the number of biocontrol bacteria in the matrix carrier is ensured to reach 1100CFU/gDW carrier matrix.
The invention provides a method for eliminating continuous cropping obstacles of melons by using biocontrol bacterial manure prepared by bacteria, which specifically comprises the following steps:
a. the method comprises the following steps of taking bacillus subtilis separated from a vineyard as a strain, preparing biocontrol bacterial manure, and uniformly spreading the prepared biocontrol bacterial manure to melon fields according to 136 kg/mu one month before melon seedlings are transplanted;
b. in the fallow period, 60 kg/667 m of soil for planting the melons is scattered2Lime nitrogen and 60 kg/667 m2Then turning over, leveling, sealing and watering, and maintaining sealing temperatureWatering at 450 deg.C for 25 days, covering with white film on the soil to make the temperature of planting area reach above 50 deg.C, and sealing the shed for 35 days;
c. after the step b is finished, removing the plastic film, performing ventilation treatment, then ploughing, performing ventilation treatment, and sprinkling 136 kg/mu of biocontrol bacterial fertilizer and 450 kg/667 m2Carrying out rotary tillage and uniform mixing on the biological fertilizer, ridging, covering a black film, and planting the cultured sweet melon seedlings in soil; and in the growth process of the melons, the amino acid foliar fertilizer is sprayed to the melons once every 11 days.
The invention can effectively inhibit the growth of soil fungi through biocontrol bacteria, promote the growth of soil bacteria, balance the balance of soil microbial communities, has the function of decomposing coumaric acid which is the main component of melon root secretion, can reduce or even eliminate phenolic acid substances in continuous cropping soil, adopts biocontrol bacterial manure produced by rice bran, wheat bran or corn crushed particles, is applied to the melon continuous cropping soil, can bring biocontrol bacteria into the soil and bring a large amount of organic matters into the soil, can improve the physical and chemical properties of the soil and the health state of the continuous cropping soil, has simple process, easily obtained raw materials, low price and simple operation, is stable and safe in bacterial manure products, can kill most pathogenic bacteria in the soil by applying lime nitrogen and quicklime into the soil, effectively reduces the occurrence of soil-borne diseases, sprays amino acid foliar fertilizer, promotes the growth of crops and improves the quality of the crops, and the spraying is carried out in the melon growth period, so that the quality of the melon can be improved, and the yield of the melon can be increased.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (7)

1. A method for preparing biocontrol bacterial fertilizer by bacteria is characterized by comprising the following steps:
a. taking bacillus subtilis separated in a vineyard as a strain, and separating and identifying the strain;
b. sterilizing the carrier matrix;
c. b, adding sterilized soybean meal, ammonium nitrate, cane sugar, magnesium sulfate, calcium sulfate and manganese sulfate into the carrier matrix in the step b, adding sterile water and seed liquid of biocontrol bacteria, and fully and uniformly stirring to obtain a fermentation raw material;
d. and c, fermenting the fermentation raw material obtained in the step c to ensure that the number of biocontrol bacteria in the substrate carrier reaches 1100CFU/gDW carrier substrate.
2. The method as claimed in claim 1, wherein the carrier matrix in step b is rice bran, wheat bran or corn material, and the bacteria are sterilized by moist heat at 80-100Kpa and 130 ℃ in step b.
3. The method for preparing the biocontrol bacterial fertilizer by using the bacteria as claimed in claim 1, wherein in the step c, the soybean meal, the ammonium nitrate, the sucrose, the magnesium sulfate, the calcium sulfate and the manganese sulfate are sequentially used in an amount of 1% -3%, 2% -4%, 0.2% -0.5%, 0.1% -0.2%, 0.02% -0.06% and 0.02% -0.05% of the weight of the fermentation substrate, the amount of sterile water is 50% -65% of the weight of the carrier substrate, the amount of biocontrol bacterial seed liquid is 1% -1.2% of the weight of the carrier substrate, the total number of bacterial colonies is 4 x 110CFU/mL, and the biocontrol bacterial seed liquid is fresh bacterial liquid with the storage time of not more than 36-48 h.
4. The method for preparing biocontrol bacterial manure through bacteria as claimed in claim 1, wherein the fermentation of the fermentation raw material in the step d is performed in a thermal insulation device, the initial temperature is set to 30-38 ℃, the fermentation time is 20-24h, and the ventilation rate in the high temperature period is adjusted to 0.13-0.16m3/(m3min), ensuring that the temperature of the carrier matrix is maintained to be 38-46 ℃, and fermenting for 48 h.
5. A method for eliminating continuous cropping obstacles of melons by using biocontrol bacterial manure prepared by bacteria is characterized by comprising the following steps:
a. the method comprises the steps of taking bacillus subtilis separated from a vineyard as a strain, preparing a biocontrol bacterial fertilizer, and uniformly spreading the prepared biocontrol bacterial fertilizer to a melon field according to 138 kg/mu of 134-year-old seeds one month before melon seedling transplantation;
b. spreading lime nitrogen and quicklime into the melon planting soil during the fallow period, turning over, leveling, sealing and watering, covering the soil with a white film after watering to enable the planting area temperature to reach more than 55 ℃, and sealing the greenhouse for 26-35 days;
c. b, after the step b is finished, removing the plastic film, performing ventilation treatment, then performing ploughing, performing ventilation treatment, spraying bio-control bacterial fertilizer and bio-fertilizer, performing rotary tillage, uniformly mixing, ridging, covering a black film, and planting the cultivated sweet melon seedlings in soil; and in the growth process of the melons, the amino acid foliar fertilizer is sprayed to the melons once every 9 to 14 days.
6. The method for preparing biocontrol bacterial manure by using bacteria as claimed in claim 5, wherein pathogen-free nutrient soil is selected for muskmelon field in steps a and c, and 1500-.
7. The method for preparing biocontrol bacterial manure by bacteria as claimed in claim 5, wherein the spreading amount of lime nitrogen and quicklime in the step b are respectively as follows: lime nitrogen 50-60 kg/667 m250-60 kg of quicklime/667 m2。
CN202110823099.5A 2021-07-21 2021-07-21 Method for preparing biocontrol bacterial manure by bacteria and method for eliminating continuous cropping obstacles of melons Pending CN113336601A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101508601A (en) * 2009-03-31 2009-08-19 孙明坤 Microorganism organic fertilizer and method of preparing the same
CN101676225A (en) * 2008-09-16 2010-03-24 佛山市楚一科技有限公司 A new microbe agent and preparation method thereof
CN109220035A (en) * 2018-08-03 2019-01-18 南京市蔬菜科学研究所 A kind of control method of muskmelon continuous cropping obstacle
CN109836190A (en) * 2019-02-18 2019-06-04 曹翠玲 A method of biocontrol bacterial fertilizer is prepared using biocontrol bacteria and eliminates muskmelon continuous cropping obstacle

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101676225A (en) * 2008-09-16 2010-03-24 佛山市楚一科技有限公司 A new microbe agent and preparation method thereof
CN101508601A (en) * 2009-03-31 2009-08-19 孙明坤 Microorganism organic fertilizer and method of preparing the same
CN109220035A (en) * 2018-08-03 2019-01-18 南京市蔬菜科学研究所 A kind of control method of muskmelon continuous cropping obstacle
CN109836190A (en) * 2019-02-18 2019-06-04 曹翠玲 A method of biocontrol bacterial fertilizer is prepared using biocontrol bacteria and eliminates muskmelon continuous cropping obstacle

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