CN113318038A - Acne-removing and anti-inflammatory sophora alopecuroide total alkaloid mask and preparation method thereof - Google Patents

Abstract

The invention relates to the field of preparation of daily necessities, in particular to a acne-removing and anti-inflammatory sophora alopecuroide total alkaloid mask and a preparation method thereof, wherein the mask comprises the following components in percentage by mass: 5% of vaseline, 2.5% of octadecanol, 0.03% of EDTA disodium, 3% of butanediol, 0.3% of triethanolamine, 3% of glycerol, 0.25% of xanthan gum, 2% of glycerol stearyl alcohol, 1% of cetostearyl alcohol, 0.5% of stearic acid, 2% of simethicone, 8% of liquid paraffin, 0.1% of ethylparaben, 0.02% of essence, 0.1% of VE, 11.43% of total alkali extract of sophora alopecuroides and the balance of purified water. The mask of the invention has good moisture retention and anti-inflammation, and specifically comprises the following components: the used raw materials are verified to have no irritation to animal skin through skin acute toxicity experiments, so that the facial skin is protected; the extract of Chinese medicinal materials is used as main material, and is combined with glycerol to prevent excessive water loss and moisturize facial skin.

Description

Acne-removing and anti-inflammatory sophora alopecuroide total alkaloid mask and preparation method thereof

Technical Field

The invention relates to the field of preparation of daily necessities, in particular to a sophora alopecuroide total alkaloid mask for removing acne and resisting inflammation and a preparation method thereof.

Background

Herba Sophorae Alopecuroidis (L) has effects of clearing away heat and toxic materials, dispelling pathogenic wind, removing dampness, resisting bacteria, relieving inflammation, relieving pain, killing parasite, etc., and has high medicinal value, but can not be taken in excess. Zhang Y et al (2006) reported that sophoridine has a significant inhibitory activity against coxsackie group B type 3 virus (CVB3) by modulating cytokine expression. Li CQ et al (2005) reported that liposomal matrine can significantly inhibit hepatitis B virus replication in vitro and in vivo. The Wan CX (2015) research shows that the flavonoid compound has remarkable antibacterial and mycoderm inhibiting effects, and the Minimum Inhibitory Concentration (MIC) of the flavonoid compound to staphylococcus epidermidis is 3.1-12.5 ug/mL. Zhou X et al (2012) reported that Sophora alopecuroides total alkaloids inhibited drug-resistant Escherichia coli (ampicillin, amikacin, cefotaxime and ciprofloxacin). Tian A and the like (2014) find that the aloperine has obvious inhibition effect on the inflammatory reaction caused by helicobacter pylori.

At present, the preparations developed by taking sophora alopecuroides as raw materials in China mainly comprise cathartic tablets, gynecological inflammation suppository, sophora alopecuroide injection, sophora alopecuroide capsules, compound sophora alopecuroide oral ulcer composite membranes, sophora alopecuroide oil liniment, sophora alopecuroide cream, sophora alopecuroide total alkali coating films, sophora alopecuroide total alkali temperature-sensitive gels, sophora alopecuroide total alkali transdermal preparations and the like.

At present, a plurality of acne-removing skin care products such as cleansing products, astringent, cream, emulsion, essence, facial masks and the like are available on the market. The acne-removing skin care product is common facial masks, wherein the common facial masks mainly comprise patch type facial masks, paste type facial masks and powder type cleaning facial masks. The commercially available acne-removing mask mainly plays a role by adding the following five types of functional components: the first category is cutin treating agent, such as salicylic acid (safe use concentration is 0.5% -2%), alpha-hydroxy acid (glycolic acid, lactic acid, citric acid), white willow extract, azelaic acid, papain, etc., to remove superfluous cutin cell of epidermis and dredge pore. The second category is sebum secretion inhibitor, such as zinc salt (zinc sulfate, zinc gluconate, zinc glycyrrhetate, zinc pyrithione), sulfur, vitamin B6, vitamin B3, nicotinamide, soybean isoflavone, tanshinone extract, which can reduce or inhibit excessive secretion of sebum, prevent hair follicle obstruction, and prevent mass propagation of Propionibacterium acnes. The third category is a bactericide, and commonly used bactericidal components comprise zinc salts (zinc sulfate, zinc gluconate, zinc glycyrrhetate and zinc pyrithione), sulfur, azelaic acid, sweet wormwood volatile oil, clove essential oil, rosemary essential oil, tea tree essential oil, propolis, capsaicin and antibacterial peptide, can quickly kill skin parasitic flora such as propionibacterium acnes, mites, pityrosporum orbiculare, staphylococcus aureus and the like, and prevent inflammation and acne growth caused by bacterial pathogen erosion. The fourth group is anti-inflammatory agent, and the common components comprise tea tree extract, delphinium extract, hamamelis extract, etc., and can inhibit inflammatory factors in hair follicles and avoid papules, pustules and severe pimples of nodules. The fifth category is whitening agents to remove residual scars from whelk, and the commonly used whitening agents include arbutin, azelaic acid and derivatives thereof, glucosamine, licorice extract, linoleic acid, vitamin C derivatives, vitamin E derivatives, green tea extract, and the like.

Therefore, most of the existing acne-removing facial masks rely on chemical additives to remove acnes, the normal flora of the skin can be damaged, the use of hormones such as chloramphenicol and the like is easy to form paralysis or pigmentation, severe or even cause skin allergy, and the pathogenic bacteria are easy to generate drug resistance, so that the facial skin state can be improved only in a short period, and the facial skin rebounds once the facial mask is stopped, even has dependence, the problem of the facial skin cannot be thoroughly solved, and the effect is poor. Some of the traditional Chinese medicine facial masks sold in the market have large single dosage and have less obvious effect.

Disclosure of Invention

The invention aims to provide a sophora alopecuroide total alkaloid mask with acne removing and anti-inflammatory functions and a preparation method thereof.

In order to achieve the purpose, the invention adopts the technical scheme that:

the acne-removing and anti-inflammatory sophora alopecuroide total alkaloid mask comprises the following components in percentage by mass: 5% of vaseline, 2.5% of octadecanol, 0.03% of EDTA disodium, 3% of butanediol, 0.3% of triethanolamine, 3% of glycerol, 0.25% of xanthan gum, 2% of glycerol stearyl alcohol, 1% of cetostearyl alcohol, 0.5% of stearic acid, 2% of simethicone, 8% of liquid paraffin, 0.1% of ethylparaben, 0.02% of essence, 0.1% of VE, 11.43% of total aloperine and the balance of purified water.

The invention also discloses a preparation method of the acne-removing and anti-inflammatory sophora alopecuroide total alkaloid mask, which comprises the following steps:

s1, preparing the sophora alopecuroide total alkali extract:

pulverizing herba Sophorae Alopecuroidis, extracting with ethanol to obtain herba Sophorae Alopecuroidis ethanol extractive solution, concentrating, and recovering ethanol to obtain total alkali solution of herba Sophorae Alopecuroidis; adding a proper amount of acid into the sophora alopecuroide total alkali solution to obtain a neutralized solution, separating and purifying to obtain sophocarpidine, sophocarpidine neutralized solution and sophoridine neutralized solution, adding a certain amount of toluene into the sophoridine neutralized solution for extraction, concentrating and recovering the toluene after extraction to obtain a biological alkali solution, adding ethanol into the biological alkali solution, filtering to obtain sophora alopecuroide biological total alkali solution, and concentrating the sophora alopecuroide biological total alkali solution at the temperature of below 60 ℃ to obtain a paste to obtain sophora alopecuroide total alkali extract;

s2, weighing the following components in percentage by mass: 5% of vaseline, 2.5% of octadecanol, 0.03% of EDTA disodium, 3% of butanediol, 0.3% of triethanolamine, 3% of glycerol, 0.25% of xanthan gum, 2% of glycerol stearyl alcohol, 1% of cetostearyl alcohol, 0.5% of stearic acid, 2% of simethicone, 8% of liquid paraffin, 0.1% of ethylparaben, 0.02% of essence, 0.1% of VE and 11.43% of total alkali extract of sophora alopecuroides, and the balance of purified water;

s3, stirring and mixing the weighed vaseline, octadecanol, EDTA disodium, butanediol, triethanolamine, glycerol and xanthan gum at 80 ℃ to obtain a mixed solution A;

s4, mixing and stirring the weighed glycerol stearyl alcohol, cetearyl alcohol, stearic acid, simethicone and liquid paraffin at 80 ℃ to obtain a mixed solution B;

s5, slowly adding the mixed liquid B in the step S4 into the mixed liquid A, stirring, adding ethylparaben, essence and VE while stirring, uniformly stirring to obtain a mixed liquid C, and cooling to 37 ℃;

s6, adding the weighed total aloperine extract into the mixed solution C, uniformly stirring, and standing at 37 ℃ for 30min to obtain the mask.

The invention has the following beneficial effects:

1) the mask of the invention has good moisture retention and anti-inflammation, and specifically comprises the following components: the used raw materials are verified to have no irritation to animal skin through skin acute toxicity experiments, so that the facial skin is protected; the extract of Chinese medicinal materials is used as main material, and is combined with glycerol to prevent excessive water loss and moisturize facial skin.

2) The mask disclosed by the invention can improve the inflammation of the facial skin, has the acne removing effect, does not generate dependence, is simple in preparation process and is convenient to produce.

Detailed Description

The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.

Example 1

The acne-removing and anti-inflammatory sophora alopecuroide total alkaloid mask is prepared by the following steps:

s1, preparing the sophora alopecuroide total alkali extract:

pulverizing herba Sophorae Alopecuroidis, extracting with ethanol to obtain herba Sophorae Alopecuroidis ethanol extractive solution, concentrating, and recovering ethanol to obtain total alkali solution of herba Sophorae Alopecuroidis; adding a proper amount of acid into the sophora alopecuroide total alkali solution to obtain a neutralized solution, separating and purifying to obtain sophocarpidine, sophocarpidine neutralized solution and sophoridine neutralized solution, adding a certain amount of toluene into the sophoridine neutralized solution for extraction, concentrating and recovering the toluene after extraction to obtain a biological alkali solution, adding ethanol into the biological alkali solution, filtering to obtain sophora alopecuroide biological total alkali solution, and concentrating the sophora alopecuroide biological total alkali solution at the temperature of below 60 ℃ to obtain a paste to obtain sophora alopecuroide total alkali extract;

s2, weighing the following components in percentage by mass: 5% of vaseline, 2.5% of octadecanol, 0.03% of EDTA disodium, 3% of butanediol, 0.3% of triethanolamine, 3% of glycerol, 0.25% of xanthan gum, 2% of glycerol stearyl alcohol, 1% of cetostearyl alcohol, 0.5% of stearic acid, 2% of simethicone, 8% of liquid paraffin, 0.1% of ethylparaben, 0.02% of essence, 0.1% of VE and 11.43% of total alkali extract of sophora alopecuroides, and the balance of purified water;

s3, stirring and mixing the weighed vaseline, octadecanol, EDTA disodium, butanediol, triethanolamine, glycerol and xanthan gum at 80 ℃ to obtain a mixed solution A;

s4, mixing and stirring the weighed glycerol stearyl alcohol, cetearyl alcohol, stearic acid, simethicone and liquid paraffin at 80 ℃ to obtain a mixed solution B;

s5, slowly adding the mixed liquid B in the step S4 into the mixed liquid A, stirring, adding ethylparaben, essence and VE while stirring, uniformly stirring to obtain a mixed liquid C, and cooling to 37 ℃;

s6, adding the weighed total aloperine extract into the mixed solution C, uniformly stirring, and standing at 37 ℃ for 30min to obtain the mask.

Evaluation of skin and eye toxicity

The experimental design is shown in table 1:

acute transdermal (intact skin) toxicity test: rat smearing (one-time contamination, 30 cm)^2_40 cm²Once 0.5 g), sealing and contacting for 24h, and removing residual test substances by using water after the infection is finished. Contamination 1 d: the experimental animals were observed for the change of toxic manifestations and death by hair and skin, eyes and mucous membranes, as well as respiratory, circulatory, autonomic and central nervous systems, limb movements and behavioural activities, and the like, with particular attention paid to the presence of symptoms such as tremor, convulsions, salivation, diarrhea, lethargy, and coma. Weighing was done weekly, surviving animals were weighed at the end of the observation period, and necropsy was performed after sacrifice. The observation period is as follows: 14 d.

Acute transdermal (damaged skin) toxicity test: rat smearing (one-time contamination, 30 cm)^2_40 cm²Once 0.5 g), sealing contact for 2 h.

After the contamination is finished, the residual test substance is removed by using water. Contamination 1 d: experimental animals were observed for erythema and eschar formation, edema formation and death were weighed weekly, surviving animals at the end of the observation period were weighed, and necropsy was performed after sacrifice. The observation period is as follows: 14 d.

Acute eye irritation test: rat eyeballs (0.1 g infected once) are dripped into facial mask liquid, the eyes are closed for 1s, and when the eye reaches the 30 th s, the eyes of the animal are washed for 30s by water flow which is enough and fast in flow speed and can not cause the injury of the eyes of the animal, so that the eyes of the animal are checked. (cornea, iris, conjunctiva). After the end of the 24h observation and recording, the eyes of all animals were further examined using sodium fluorescein.

Results and evaluation of acute transdermal (intact skin) toxicity test: the rats with low, medium, high and extremely high doses have no poisoning and death phenomena in the experimental observation period, the hair and skin, eyes and mucous membranes of the rats, the breathing, circulation, autonomic nerves, central nervous system, limb movement, behavior activity and the like are normal, and symptoms such as tremor, convulsion, salivation, diarrhea, lethargy, coma and the like do not appear. The body weight of each group of rats remained steadily increasing within 14 days. Sophora alopecuroides total alkaloid facial mask LD₅₀More than or equal to 2180mg/kg, and the skin toxicity classification belongs to a micro-toxicity level according to the technical specification for cosmetic safety 2015 edition.

Test results and evaluation of acute percutaneous (damaged skin) toxicity test: the skin recovers faster at low and medium dose during the test observation period, and the skin erythema disappears after 48h and basically recovers to the normal skin level. The high and extremely high dose skin recovery was slow, eschar disappeared after 7 days in the high dose group, the skin returned to a normal level basically, and a small part of the skin remained after 14 days in the extremely high dose group. The body weight of each group of rats remained steadily increasing within 14 days. The average integral of each animal per day = Σ 0/16/14=0, and the total alkaloids sophora alopecuroides mask has no irritation to damaged skin according to the skin irritation intensity classification of "technical specification for cosmetic safety 2015 edition".

Acute eye irritation test results and evaluation: no injury occurs to the cornea, iris and conjunctiva of rats with low, medium, high and extremely high doses in the experimental observation period, according to the evaluation of the highest integral mean value and recovery time of the stimulation response of the cornea, iris or conjunctiva of animals at the observation time point of 24, 48 or 72 h after a test object in the cosmetic safety technical specification 2015 edition, a cosmetic product, the low, medium, high and extremely high groups of integrals are all zero through calculation, so that the sophora alopecuroides total alkaloid mask has no stimulation to the eyeball.

Anti-inflammatory evaluation

Experiment for mouse auricle swelling caused by xylene

40 healthy ICR mice were taken, weighing 24. + -.2 g, and half each male and female. The test result is randomly divided into 5 groups, namely a model group, a control group, a sophora alopecuroide paste mask high-dose group, a sophora alopecuroide paste mask medium-dose group and a sophora alopecuroide paste mask low-dose group, wherein each group comprises 8. Applying Sophora alopecuroides ointment facial mask (high, medium and low dosage), common facial mask and normal saline on two sides of mouse right ear at the same time every day, 1 time every day, massaging properly to promote absorption, and continuously applying for 8 days. After the final application of the face mask for 1h on day 8, 20 μ L of xylene was precisely absorbed, and the right ear of the mouse was evenly coated with xylene, while the left ear was used as a control without treatment. After applying the drug to the right ear for 30min, 5 groups of mice were sacrificed by dislocation of cervical vertebrae, after the mice were sacrificed, each was cut off two ears along the baseline of auricle, the edges of the two ears were aligned, the ears were punched out at the symmetrical parts of the left and right ears by a stainless steel punch with a diameter of 6 mm, and the weights of the left and right ears were weighed by a precision analytical balance, respectively. And calculating the mass difference, swelling rate and swelling inhibition rate of the left and right ear plates of the mouse. Degree of swelling of auricle (difference in mass between left and right ear pieces) = mass of right ear-mass of left ear; swelling rate (%) = (right ear mass-left ear mass)/left ear mass × 100%; swelling inhibition (%) = (average swelling in model group-average swelling degree in administered group)/average swelling degree in model group × 100%.

Experiment for swelling of toes of mice caused by fresh egg white

The experimental groups are as above, the mouse feet are smeared with Sophora alopecuroides ointment facial mask (high, medium and low dosage), common facial mask and normal saline at the same time every day for 1 time every day, and after smearing, the mouse feet are properly massaged to promote absorption and are smeared continuously for 8 days. After the mask is smeared for 60 min at the end of the day 8, 0.02mL of 50% fresh egg white solution is injected subcutaneously into right hind foot metatarsal joints of each group of mice to induce inflammation, the cervical vertebrae of the mice are dislocated and killed 30min after inflammation, the left and right feet of the mice are aligned and cut down along the ankle joints, and the mass of the left and right feet is respectively weighed by a precision analytical balance. The weight difference, swelling rate and swelling inhibition rate of the left and right feet of the mice were calculated.

Toe swelling and distension = right foot mass-left foot mass;

swelling rate = right foot mass-left foot mass/left foot mass × 100%;

swelling inhibition (%) = (average swelling in model group-average swelling degree in administered group)/average swelling degree in model group × 100%.

The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.

Claims (2)

1. The acne-removing and anti-inflammatory sophora alopecuroide total alkaloid facial mask is characterized in that: comprises the following components in percentage by mass: 5% of vaseline, 2.5% of octadecanol, 0.03% of EDTA disodium, 3% of butanediol, 0.3% of triethanolamine, 3% of glycerol, 0.25% of xanthan gum, 2% of glycerol stearyl alcohol, 1% of cetostearyl alcohol, 0.5% of stearic acid, 2% of simethicone, 8% of liquid paraffin, 0.1% of ethylparaben, 0.02% of essence, 0.1% of VE, 11.43% of total alkali extract of sophora alopecuroides and the balance of purified water.

2. A preparation method of a sophora alopecuroide total alkaloid mask with acne removing and anti-inflammatory functions is characterized by comprising the following steps: the method comprises the following steps:

s1, preparing the sophora alopecuroide total alkali extract:

pulverizing herba Sophorae Alopecuroidis, extracting with ethanol to obtain herba Sophorae Alopecuroidis ethanol extractive solution, concentrating, and recovering ethanol to obtain total alkali solution of herba Sophorae Alopecuroidis; adding a proper amount of acid into the sophora alopecuroide total alkali solution to obtain a neutralized solution, separating and purifying to obtain sophocarpidine, sophocarpidine neutralized solution and sophoridine neutralized solution, adding a certain amount of toluene into the sophoridine neutralized solution for extraction, concentrating and recovering the toluene after extraction to obtain a biological alkali solution, adding ethanol into the biological alkali solution, filtering to obtain sophora alopecuroide biological total alkali solution, and concentrating the sophora alopecuroide biological total alkali solution at the temperature of below 60 ℃ to obtain a paste to obtain sophora alopecuroide total alkali extract;

s2, weighing the following components in percentage by mass: 5% of vaseline, 2.5% of octadecanol, 0.03% of EDTA disodium, 3% of butanediol, 0.3% of triethanolamine, 3% of glycerol, 0.25% of xanthan gum, 2% of glycerol stearyl alcohol, 1% of cetostearyl alcohol, 0.5% of stearic acid, 2% of simethicone, 8% of liquid paraffin, 0.1% of ethylparaben, 0.02% of essence, 0.1% of VE and 11.43% of total alkali extract of sophora alopecuroides, and the balance of purified water

S3, stirring and mixing the weighed vaseline, octadecanol, EDTA disodium, butanediol, triethanolamine, glycerol and xanthan gum at 80 ℃ to obtain a mixed solution A;

s4, mixing and stirring the weighed glycerol stearyl alcohol, cetearyl alcohol, stearic acid, simethicone and liquid paraffin at 80 ℃ to obtain a mixed solution B;

s5, slowly adding the mixed liquid B in the step S4 into the mixed liquid A, stirring, adding ethylparaben, essence and VE while stirring, uniformly stirring to obtain a mixed liquid C, and cooling to 37 ℃;

s6, adding the weighed total aloperine extract into the mixed solution C, uniformly stirring, and standing at 37 ℃ for 30min to obtain the mask.