CN113249197A

CN113249197A - System for preparing microorganism mass spectrum identification sample

Info

Publication number: CN113249197A
Application number: CN202110499877.XA
Authority: CN
Inventors: 陈涛; 张静; 林金飞; 任静; 王珊霞; 代新; 严喜平; 李德胜
Original assignee: Nanxinyi Guangzhou Manufacturing Co ltd
Current assignee: Nanxinyi Guangzhou Manufacturing Co ltd
Priority date: 2021-05-08
Filing date: 2021-05-08
Publication date: 2021-08-13

Abstract

The invention discloses a system for preparing a microorganism mass spectrum identification sample, which comprises a raw material placing module, a sample application module, a moving module, a control module and a shell, wherein the raw material placing module comprises a bottom base, a culture dish placing area and a target plate placing area are arranged on the bottom base, a target plate placing mechanism is arranged on the target plate placing area, the target plate placing mechanism comprises a heating film and an inner side positioning block, the sample application module comprises a support, an acquisition assembly, a first moving module and a heating device, the control module comprises an electric controller and an identification mechanism, and a protective door capable of being opened and closed is arranged on the shell. Remove module drive support and subbase relative motion, make the inoculating needle get the fungus back on the culture dish, remove and scribble to establish the formation bacterial colony on the target plate, the device heating inoculating needle that generates heat in order self-sterilizer after the spotting is accomplished, and the heating film also starts to make the target plate heat up, and the bacterial colony forms the sample on the target plate. The invention can complete the preparation of the sample without manual operation, reduce the pollution in the preparation process and improve the preparation efficiency.

Description

System for preparing microorganism mass spectrum identification sample

Technical Field

The invention relates to a system for preparing a microorganism mass spectrum identification sample in the technical field of biomedical instruments.

Background

The main task of a clinical microorganism laboratory is to assist a clinician to diagnose and identify various infectious diseases and guide the clinical timely and reasonable administration of the medicines, and the culture of pathogenic microorganisms usually needs a long time and is easy to delay the timely treatment of a patient, so that the rapid and accurate identification of infected strains is the key for determining whether the patient can be treated timely.

The Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology determines the type of the microorganism by detecting the specific fingerprint spectrum of the microorganism and then performing matching analysis with a database. Compared with the traditional phenotype identification and molecular biology technology, MALDI-TOF MS is rapid, accurate and low in cost, and a database can be continuously updated and perfected, so that the identification efficiency of clinical microorganisms, particularly microorganisms difficult to culture such as microaerophilic bacteria, anaerobic bacteria, mycobacteria and fungi, is greatly improved, and the technology is widely recognized as a milestone for rapid identification of microorganisms in less than 10 years of clinical application.

The pretreatment of the microorganism specimen is an important link in the inspection, the workload is large, the time spent is long, the operator faces the risk of biological infection, and the manual operation is greatly influenced by human factors, so that the accuracy of all subsequent detections is directly influenced. Therefore, a full-automatic microbial sample processing system with standard processing capacity is needed in the market, which can complete the full-automatic, fast and safe inoculation of bacterial colonies from a culture dish to a MALDI-TOF MS target plate, and the lysis and immobilization processing.

Disclosure of Invention

The invention aims to solve at least one technical problem in the prior art and provides a system for preparing a microorganism mass spectrometry identification sample, which can quickly and automatically prepare a mass spectrometry sample.

According to an embodiment of the invention, a system for preparing a microorganism mass spectrometry identification sample is provided, which comprises a raw material placing module, wherein the raw material placing module comprises a bottom base, a culture dish placing area and a target plate placing area are arranged on the bottom base, a target plate placing mechanism is arranged on the target plate placing area, the target plate placing mechanism comprises a heating film and an inner side positioning block, the top of the inner side positioning block is used for supporting a target plate, and the heating film is arranged between the inner side positioning block and the bottom base; the sample application module comprises a support, a collection assembly, a first moving module and a heating device, wherein the collection assembly comprises a mounting substrate and an inoculating needle fixed on the mounting substrate, the inoculating needle is connected with the heating device, the mounting substrate is movably connected on the support, the first moving module is connected with the mounting substrate, and the first moving module is used for driving the inoculating needle to displace in the vertical direction; a moving module connected to the bottom base or the stand, the moving module for driving relative movement of the stand and the bottom base; the control module comprises an electric controller and an identification mechanism, the identification mechanism is used for identifying the culture dish on the culture dish placing area, the identification mechanism is electrically connected with the electric controller, and the electric controller is respectively and electrically connected with the raw material placing module, the sample application module and the moving module; the sample application device comprises a shell, wherein a protective door capable of being opened and closed is arranged on the shell, a cavity is formed in the shell, and a raw material placing module, a sample application module, a moving module and an identification mechanism are all arranged in the cavity.

According to the embodiment of the present invention, further, the housing has an opening, the protection door is installed on the opening, a door power mechanism is disposed in the cavity, the door power mechanism includes a first moving assembly and a second moving assembly, the first moving assembly is installed on an output end of the second moving assembly, an output end of the first moving assembly is connected to the protection door, the first moving assembly is configured to change a distance between the protection door and the opening, and the second moving assembly is configured to drive the protection door to move away from or close to the opening in a translational manner.

According to the embodiment of the invention, further, the spotting module further comprises a guiding mechanism, the guiding mechanism comprises a sliding rail and a sliding part which are matched with each other, the sliding rail is fixed on the bracket, the sliding part is connected with the mounting substrate, and the output end of the first moving module is connected with the sliding part.

According to the embodiment of the invention, further, the number of the inoculating needles is more than two, the mounting substrate is movably mounted on the sliding member, the spotting module further comprises a switching power member, the switching power member is connected with the mounting substrate, the switching power member is used for driving the mounting substrate to move along a preset direction, and the inoculating needles are arranged at intervals along the preset direction.

According to an embodiment of the present invention, further, a reagent placing area is further disposed on the bottom base, and the collecting assembly further includes at least two injection needles, and the injection needles are arranged at intervals along a preset direction.

According to an embodiment of the present invention, the control module further includes a positioning mechanism electrically connected to the electric controller, the positioning mechanism includes a positioning camera and a monitoring assembly, the positioning camera is used for positioning the position of the target plate and the position of the bacterial colony in the culture dish, and the monitoring assembly is used for positioning the positions of the inoculating needle and the injection needle.

According to the embodiment of the invention, further, two oppositely arranged positioning stop blocks are arranged on the culture dish placing area, and a culture dish placing space capable of accommodating the culture dish is formed between the two positioning stop blocks.

According to an embodiment of the present invention, the target board placing mechanism further includes a target board seat, a first groove capable of accommodating the target board is disposed at a top of the target board seat, a second groove capable of being sleeved on the inner side positioning block is disposed at a bottom of the target board seat, and the first groove is communicated with the second groove.

According to the embodiment of the present invention, further, the moving module includes a second moving module and a third moving module, the third moving module is connected to an output end of the second moving module, the bottom base is connected to an output end of the third moving module, and a preset included angle is formed between a moving direction of the second moving module and a moving direction of the third moving module.

According to an embodiment of the present invention, further, a sterilization mechanism is provided in the cavity.

The invention has the beneficial effects that: the movable module drives the support and the base to move relatively, so that the inoculating needle is moved to the target plate to form a bacterial colony after being used for taking bacteria on the culture dish, the heating device heats the inoculating needle to automatically sterilize after the sample application is finished, the heating film is started to heat the target plate, and the bacterial colony forms a sample on the target plate. The method does not need manual operation, can be widely applied to operations such as colony inoculation, preparation of a microorganism mass spectrum identification target plate and the like, reduces pollution in the preparation process and improves the preparation efficiency.

Drawings

In order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly described below. It is clear that the described figures are only some embodiments of the invention, not all embodiments, and that a person skilled in the art can also derive other designs and figures from them without inventive effort.

FIG. 1 is a schematic external view of an embodiment of the present invention;

FIG. 2 is a schematic view of a guard door and door actuating mechanism according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of a material placement module in an embodiment of the present invention;

FIG. 4 is a schematic diagram of a mobile module in an embodiment of the invention;

FIG. 5 is a schematic view of a deposition module in an embodiment of the invention;

FIG. 6 is a schematic diagram of a control module in an embodiment of the invention.

Detailed Description

Reference will now be made in detail to the present preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to like elements throughout.

In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, the meaning of a plurality of means is one or more, the meaning of a plurality of means is two or more, and larger, smaller, larger, etc. are understood as excluding the number, and larger, smaller, inner, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.

Referring to fig. 1 to 6, the system for preparing a microorganism mass spectrometry identification sample in an embodiment of the present invention includes a housing 10, a raw material placing module, a sample application module, a moving module, and a control module, wherein a protective door 20 capable of being opened and closed is disposed on the housing 10, a cavity is formed in the housing 10, and the raw material placing module, the sample application module, and the moving module are disposed in the cavity. The raw material placing module comprises a bottom base 30, and a culture dish placing area and a target plate placing area are arranged on the bottom base 30. It will be readily understood that reference to the target plate 31 in this application is not only to a target plate dedicated to mass spectrometry but can also be any plate-like or non-plate-like container capable of carrying a microbiological sample. The moving module is connected to the raw material placing module or the sample application module and is used for driving the sample application module and the raw material placing module to move relatively. The control module controls the sample application module to collect strains from the culture dish 32 and inoculate the strains to the target plate 31 to form an identification sample, and finally the identification sample can be taken out by opening the protective door 20. The system for preparing the microorganism mass spectrum identification sample can be applied to the preparation of a microorganism mass spectrum identification target plate and can also be applied to the biochemical experiment fields of colony selection and inoculation and the like.

Referring to fig. 2, the housing 10 has an opening, the protective door 20 is mounted on the opening, a door actuating mechanism is disposed in the cavity, the door actuating mechanism includes a first moving assembly 22 and a second moving assembly 21, the first moving assembly 22 is mounted on an output end of the second moving assembly 21, an output end of the first moving assembly 22 is connected to the protective door 20, the first moving assembly 22 is used for changing a distance between the protective door 20 and the opening, and the second moving assembly 21 is used for driving the protective door 20 to move away from or close to the opening. The distance between the protective door 20 and the opening refers to the distance between the end surface of the protective door 20 and the plane of the opening, and the moving direction of the second moving assembly 21 is parallel to the plane of the opening. In this embodiment, the first moving assembly 22 drives the protection door 20 to move along the Y-axis, and the second moving assembly 21 drives the protection door 20 to move along the Z-axis. Two opposite sides in the opening are respectively provided with a vertical plate 23 for installing a mechanism in the door hole, and the second moving assembly 21 is fixed on the vertical plate 23. Optionally, the first moving assembly 22 is a linear sliding rail module, and a connecting frame is disposed on an inner side of the protection door 20, and the protection door 20 is connected to the linear sliding rail module through the connecting frame. The second moving assembly 21 comprises a synchronous belt and two synchronous wheels, the synchronous belt is sleeved on the two synchronous wheels, and the first moving assembly 22 and the synchronous belt are two sections. Two synchronizing wheels are first synchronizing wheel and second synchronizing wheel respectively, and first synchronizing wheel rotates and installs on a riser 23, and the second synchronizing wheel passes through the tensioning plate to be installed on same riser 23, specifically, the second passes through on the wheel rotates the installation tensioning plate, and tensioning plate slidable mounting is on this riser 23, and through the position of adjusting the tensioning plate, interval between two synchronizing wheels can be controlled to the tensioning hold-in range. The door power mechanism comprises a motor, and the wheel center of one synchronous wheel of the motor is connected to drive the synchronous belt to rotate. Further, to ensure that the vertical plate 23 is further provided with a guide slot 24, the first moving assembly 22 can cooperate with the guide slot 24, so as to ensure that the protection door 20 does not shift when moving along the Z-axis. The protective door 20 is embedded in the opening, no extra space needs to be occupied outside the shell 10, when the protective door 20 is opened, the protective door 20 moves outwards when the first moving assembly 22 is started, and when the second moving assembly 21 is started, the protective door 20 translates to expose the opening, and accordingly, the same is carried out when the protective door 20 is closed.

Referring to fig. 3, the raw material placing module includes a bottom base 30, a culture dish placing area and a target plate placing area are provided on the bottom base 30, and a target plate placing mechanism is provided on the target plate placing area. The target plate placing mechanism comprises a heating film and an inner side positioning block, the top of the inner side positioning block is used for supporting the target plate 31, the heating film is arranged between the inner side positioning block and the base seat 30, namely, two end faces of the heating film are respectively attached to the inner side positioning block and the base seat 30. The heating film can generate heat, and the heat is conducted to the target plate 31 through the inner side positioning block, so that the target plate 31 is heated. The top of inboard locating piece is the plane, and the top of inboard locating piece with can with target plate 31 direct contact to increase inboard locating piece and target plate 31's area of contact, and improve heating efficiency, improve target plate 31's the effect of being heated, the energy can be saved. It can be understood that, a temperature sensor is also installed on the inner positioning block, and the temperature sensor is used for detecting the temperature of the target plate 31 and preventing the sample itself from being damaged due to overhigh temperature.

Further, the target plate placing mechanism further comprises a target plate seat 34, a first groove capable of containing the target plate 31 is formed in the top of the target plate seat 34, a second groove capable of being sleeved on the inner side positioning block is formed in the bottom of the target plate seat 34, and the first groove is communicated with the second groove. In this embodiment, the target plate holder 34 is a hollow frame structure, the first groove and the second groove of the target plate holder 34 can be communicated, and there is no interlayer in the middle, so that the target plate 31 can directly contact with the inner measuring position block. The size of the first groove is designed according to the size of the target plate 31, so that the target plate 31 can be placed in the first groove without free movement, and the target plate 31 can be fixed quickly and conveniently; the second groove is detachably sleeved on the inner side positioning block, so that the target plate 31 and the inner side positioning block can be quickly and stably positioned. It will be readily appreciated that the target plate holder 34 has a variety of specifications, and can be adaptively replaced according to the specifications of the target plate 31, thereby improving the applicability.

In some embodiments, two positioning blocks 35 are disposed opposite to each other on the culture dish placing region, and a culture dish placing space capable of accommodating the culture dish 32 is formed between the two positioning blocks 35. Optionally, the inner side of the positioning block 35 is curved to accommodate the outer circumferential shape of the culture dish 32. The culture dish 32 is placed in the culture dish placing space, so that the culture dish 32 can be prevented from moving on the bottom base 30, and the accuracy of subsequent fungus selection is ensured.

In some embodiments, as in the present embodiment, the bottom base 30 is further provided with a reagent placement area for placing a reagent such as a culture solution, and the preparation effect of the microorganism sample can be improved by dropping the culture solution onto the target plate 31. Optionally, a plurality of reagent placing grooves 33 are arranged on the reagent placing region, and the plurality of reagent placing grooves 33 are arranged along the X axis for easy access.

Referring to fig. 5, the spotting module comprises a support 50, a collecting component, a first moving module 51 and a heating device, wherein the collecting component comprises a mounting substrate 53 and an inoculating needle 54 fixed on the mounting substrate 53, the inoculating needle 54 is connected with the heating device, the mounting substrate 53 is movably connected on the support 50, the first moving module 51 is connected with the mounting substrate 53, and the first moving module 51 is used for driving the inoculating needle 54 to displace in the vertical direction. Because the inoculating needle 54 is displaced in the vertical direction, the height of the inoculating needle 54 relative to the bottom base 30 is changed, so that the fungus picking action can be realized, and in the process, the motion track of the inoculating needle 54 can be a straight line or a curved line. The inoculating needle 54 is connected with a heating device which can heat the inoculating needle 54, or the inoculating needle 54 can automatically heat through electric conduction, so that the inoculating needle 54 can be quickly and automatically sterilized, and the next bacterium picking work can be facilitated.

Further, the sample application module further comprises a guide mechanism, the guide mechanism comprises a slide rail and a sliding part which are matched with each other, the slide rail is fixed on the support 50, the sliding part is connected with the mounting substrate 53, and the output end of the first mobile module 51 is connected with the sliding part. The sliding member always slides along the sliding rail, so that the mounting substrate 53 is limited to move linearly without deviation under the driving of the first moving module 51.

Understandably, the inoculating needle 54 comprises a nickel-chromium wire which is folded in half to form a rod-shaped structure, the total length is about 1-2 cm, and the needle head is a nickel-chromium wire bending part, so that the sampling of bacteria is convenient. The two ends of the nickel-chromium wire are connected with 24v direct current, the needle head burns red when the nickel-chromium wire is electrified, the temperature can reach 200-300 ℃, and the residual bacteria on the inoculating needle 54 can be inactivated in a short time. The inoculating needle 54 further comprises a ceramic tube which is sleeved outside the rod-like structure and can play roles of insulation, heat insulation and convenience in fixation.

In some embodiments, the number of the seeding needles 54 is two or more, the mounting substrate 53 is movably mounted on the sliding member, the spotting module further comprises a switching power member 52, the switching power member 52 is connected with the mounting substrate 53, the switching power member 52 is used for driving the mounting substrate 53 to move along a preset direction, and the seeding needles 54 are arranged at intervals along the preset direction. The switching power member 52 drives the mounting base plate 53 to move, so that each of the inoculating needles 54 can be switched to the working position. In this application, the working position refers to the position in which the tip of the inoculating needle 54 or injection needle 55 is directed towards the culture dish 32 or target plate 31. Because the number of the inoculating needles 54 is more than two, the efficiency of picking up bacteria can be effectively improved, taking two inoculating needles 54 as an example, the two inoculating needles 54 are respectively a first inoculating needle 54 and a second inoculating needle 54, and when the first inoculating needle 54 is used for picking up bacteria, the second inoculating needle 54 can be used for heating and sterilizing; after the first inoculating needle 54 finishes picking the bacteria, the second inoculating needle 54 just finishes sterilizing and is cooled to the room temperature, then the second inoculating needle 54 is switched to the working position through the switching power piece 52, and the operation is repeated in such a circulating way, so that the uninterrupted picking operation can be realized.

As an alternative embodiment, the predetermined direction is a horizontal direction, the switching power member 52 is a linear module, and the predetermined direction is parallel to the horizontal direction, specifically, the predetermined direction is parallel to the X axis, so as to correspond different inoculating needles 54 to the culture dish 32 or the target plate 31. As another alternative, in this embodiment, the predetermined direction is a closed loop direction, the switching power member 52 is a rotating module, and the mounting base plate 53 can rotate on the sliding member to switch the inoculating needle 54 to the working position, which effectively saves the mounting space.

In some embodiments, when the reagent placement region is provided on the lower base 30, the collection assembly further includes at least two injection needles 55, and the injection needles 55 are spaced apart in a predetermined direction. The switching power tool 52 can switch each injection needle 55 to the operating position, and thereby contact a reagent such as a lysis solution or a matrix solution with the target plate 31. It will be readily appreciated that each injection needle 55 is used to aspirate a different reagent injection to better accommodate the preparation of a greater variety of microorganisms.

Referring to fig. 4, a moving module is attached to the base 30 or the support 50 for driving the relative movement of the support 50 and the base 30 so that the inoculating needle 54 is movable between above the culture dish 32 and above the target plate 31 and the injecting needle 55 is movable between above the reagent placement area and above the target plate 31 to output the spawn or reagent onto the target plate 31 to form a sample. In this embodiment, the moving module is connected to the bottom base 30, so that not only the relative movement between the support 50 and the bottom base 30 can be driven, but also the bottom base 30 can be driven to extend out of the opening, thereby facilitating the supplement of the reagent or the taking and placing of the sample.

The moving module comprises a second moving module 41 and a third moving module 42, the third moving module 42 is connected to the output end of the second moving module 41, the bottom base 30 is connected to the output end of the third moving module 42, and a preset included angle is formed between the moving direction of the second moving module 41 and the moving direction of the third moving module 42. Optionally, the predetermined intersection angle is a right angle. In this embodiment, the moving direction of the second moving module 41 is parallel to the Y axis, and the moving direction of the third moving module 42 is parallel to the X axis. When the second moving module 41 drives the bottom base 30 to move along the Y-axis, the collecting assembly can move relatively between the reagent placing area, the culture dish 32 and the target plate 31 to complete the operation of picking bacteria or injecting reagent. When the third moving module 42 drives the bottom base 30 to move along the X axis, the collecting assembly can move in the X axis direction relative to the bottom base 30, so that the inoculating needle 54 can inoculate a plurality of bacterial colonies on the target plate 31 along the X axis, and the number of the bacterial colonies in the sample is increased; meanwhile, since the plurality of reagent placing grooves 33 in the reagent placing region are also arranged along the X-axis, the injection needle 55 can also aspirate different reagents.

Referring to fig. 6, the control module includes an electric controller and an identification mechanism, the identification mechanism is used for identifying the culture dish 32 on the culture dish placing area, the identification mechanism is electrically connected with the electric controller, and the electric controller is respectively electrically connected with the raw material placing module, the sample application module and the moving module. Optionally, recognition mechanism includes bar code reading camera 61, bar code reading camera 61 sets up the bottom in the culture dish space of placing, the outer bottom surface of culture dish 32 is last to be pasted the bar code, when culture dish 32 is placed in the culture dish space of placing, bar code reading camera 61's camera aligns the bar code on the outer bottom surface of culture dish 32, thereby read bacterial information in culture dish 32, bar code reading camera 61 carries culture dish 32's information to automatically controlled ware, thereby automatically controlled ware can control the relative motion of collection subassembly and base, drip different reagent to target plate 31 with fixed microorganism and preparation sample.

Further, the control module further comprises a positioning mechanism, the positioning mechanism is electrically connected with the electric controller, and the positioning mechanism comprises a positioning camera 62 and a monitoring component 63. The positioning camera 62 is used for positioning the position of the target plate 31 and the position of the colony in the culture dish 32, so that the inoculating needle 54 can accurately extend into the culture dish 32 to collect the strain and smear the strain on the target plate 31. The monitoring assembly 63 is used to locate the position of the inoculating needle 54 and the injection needle 55, and in particular the monitoring assembly 63 comprises a first monitoring camera for monitoring the position of the injection needle 55 and a second monitoring camera for monitoring the position of the inoculating needle 54, the monitoring assembly 63 being operable to feed back information on the position of the injection needle 55 and the inoculating needle 54 to the electronic controller.

In some embodiments, a sterilization mechanism is disposed within the cavity, optionally the sterilization mechanism includes at least one ultraviolet sterilization lamp. After the samples are prepared, the ultraviolet sterilizing lamp is turned on to thoroughly sterilize the cavity, so that the next sample preparation is prevented from being influenced.

The system for preparing the microorganism mass spectrometric identification sample in the embodiment has a specific use method as follows:

s1, before use, the protective door 20 is closed and filled, and an ultraviolet sterilization lamp is turned on to sterilize and disinfect the cavity.

S2. start door power unit, upward translation is in order to expose the opening after guard gate 20 outwards moves, and removal module drive end base 30Y axle direction moves in order to be close to or stretch out the opening, then puts into reagent standing groove 33 with formic acid reagent bottle, matrix reagent bottle, washing reagent pipe and waste liquid reagent bottle respectively, puts into the first recess of target plate seat 34 with target plate 31 to put into culture dish 32 and place the space.

And S3, the door power mechanism is started again to close the protective door 20, an execution program is input into the electric controller, and the system is started to operate.

S4, reading the bar code at the bottom of the culture dish 32 by the bar code reading camera 61, positioning the positions of the bacterial colonies in the target plate 31 and the culture dish 32 by the positioning camera, and sequentially selecting the bacterial colonies to be selected in the electric controller.

S5, the moving module is started to enable the formic acid solution reagent bottle on the bottom base 30 to move to the position below the collecting assembly, the rotating module drives the mounting substrate 53 to rotate so as to enable the injection needle 55 to rotate to the working position, then the first moving module 51 drives the collecting assembly to move downwards, the injection needle 55 sucks 1 mu L of formic acid solution, and then the collecting assembly ascends.

S6, the moving module is started to move the target plate 31 on the base seat 30 to the position below the acquisition assembly, and the injection needle 55 is used for dropwise adding the formic acid solution to the corresponding target point.

S7, repeating the steps S5-S6 according to the number of bacteria to be selected.

S8, the moving module is started, so that the culture dish 32 on the bottom base 30 moves to the position below the collecting assembly, the rotating module drives the mounting base plate 53 to rotate to enable the inoculating needle 54 to rotate to the working position, then the first moving module 51 drives the collecting assembly to move downwards, the inoculating needle 54 picks bacterial colonies on the culture dish 32, and then the collecting assembly rises.

S9, the moving module is started to enable the target plate 31 on the bottom base 30 to move to the position below the collecting assembly, the first moving module 51 drives the collecting assembly to move downwards so that the inoculating needle 54 can enable the microbial bacteria to fall to the center of the slaughtering target spot, the mudflat is uniform, a thin layer is formed, and then the collecting assembly rises.

S10, repeating the steps S8-S9, starting the heating device corresponding to the inoculating needle 54 used in the previous step, heating the inoculating needle 54 for sterilization, and alternately carrying out the bacteria picking and sterilizing operations on the two inoculating needles 54.

S11, after the last colony to be detected is inoculated to the target plate 31, waiting for 5min, starting a moving module to enable a matrix solution bottle on the base seat 30 to move to the position below the acquisition assembly, driving the mounting base plate 53 to rotate by a rotating module so as to enable the other injection needle 55 to rotate to a working position, after the injection needle 55 absorbs the matrix solution, starting the moving module again to enable the target plate 31 to be positioned below the acquisition assembly, dropwise adding the matrix solution to a target point coated with bacterial liquid by the injection needle 55, and then heating a film to start heating to accelerate the volatilization of the matrix;

s12, after matrix solution is dripped into each target spot, the mobile module is started to enable the cleaning reagent tube to be located below the collection assembly, after the injection needle 55 absorbs the cleaning reagent, the mobile module is started again to enable the waste liquid reagent bottle to be located below the collection assembly, and the injection needle 55 injects the cleaning solution into the waste liquid reagent bottle.

S13, after matrix solution is dripped to all target spots, the door power mechanism opens the protective door 20, the movable module pushes the base seat 30 out of the opening, the target plate 31 can be directly placed on a MALDI-TOF mass spectrometer for microorganism identification, the culture dish 32 can be recovered, and other reagents are processed according to the specification.

S14, the movable module retracts the base seat 30 into the cavity, the protective door 20 is closed, and the ultraviolet sterilizing lamp is started to sterilize and disinfect the cavity.

While the preferred embodiments of the present invention have been illustrated and described, it will be understood by those skilled in the art that the present invention is not limited to the details of the embodiments shown and described, but is capable of numerous equivalents and substitutions without departing from the spirit of the invention as set forth in the claims appended hereto.

Claims

1. A system for preparing a sample for mass spectrometric identification of a microorganism, comprising:

the raw material placing module comprises a bottom base, a culture dish placing area and a target plate placing area are arranged on the bottom base, a target plate placing mechanism is arranged on the target plate placing area, the target plate placing mechanism comprises a heating film and an inner side positioning block, the top of the inner side positioning block is used for supporting a target plate, and the heating film is arranged between the inner side positioning block and the bottom base;

the sample application module comprises a support, a collection assembly, a first moving module and a heating device, wherein the collection assembly comprises a mounting substrate and an inoculating needle fixed on the mounting substrate, the inoculating needle is connected with the heating device, the mounting substrate is movably connected on the support, the first moving module is connected with the mounting substrate, and the first moving module is used for driving the inoculating needle to displace in the vertical direction;

a moving module connected to the bottom base or the stand, the moving module for driving relative movement of the stand and the bottom base;

the control module comprises an electric controller and an identification mechanism, the identification mechanism is used for identifying the culture dish on the culture dish placing area, the identification mechanism is electrically connected with the electric controller, and the electric controller is respectively and electrically connected with the raw material placing module, the sample application module and the moving module;

the sample application device comprises a shell, wherein a protective door capable of being opened and closed is arranged on the shell, a cavity is formed in the shell, and a raw material placing module, a sample application module, a moving module and an identification mechanism are all arranged in the cavity.

2. The system for preparing a microorganism mass spectrometry identification sample of claim 1, wherein: the protective door is characterized in that an opening is formed in the shell, the protective door is installed on the opening, a door power mechanism is arranged in the cavity and comprises a first moving assembly and a second moving assembly, the first moving assembly is installed on the output end of the second moving assembly, the output end of the first moving assembly is connected with the protective door, the first moving assembly is used for changing the distance between the protective door and the opening, and the second moving assembly is used for driving the protective door to move horizontally to be far away from or close to the opening.

3. The system for preparing a microorganism mass spectrometry identification sample of claim 1, wherein: sample application module still includes guiding mechanism, guiding mechanism includes slide rail and the slider of mutually supporting, the slide rail is fixed on the support, the slider with mounting substrate connects, the output of first removal module with the slider is connected.

4. The system for preparing a microorganism mass spectrometry identification sample of claim 3, wherein: the quantity of inoculating needle is more than two, mounting substrate movable mounting be in on the slider, the application of sample module still includes switches the power spare, switch the power spare with mounting substrate is connected, it is used for the drive to switch the power spare the mounting substrate is along the direction motion of predetermineeing, each the inoculating needle is along predetermineeing direction interval arrangement.

5. The system for preparing a microorganism mass spectrometry identification sample of claim 4, wherein: still be equipped with reagent on the end base and place the district, the collection subassembly still includes two at least syringe needles, the syringe needle is arranged along predetermineeing the direction interval.

6. The system for preparing a microorganism mass spectrometry identification sample of claim 5, wherein: the control module further comprises a positioning mechanism, the positioning mechanism is electrically connected with the electric controller, the positioning mechanism comprises a positioning camera and a monitoring assembly, the positioning camera is used for positioning the position of the target plate and the position of the bacterial colony in the culture dish, and the monitoring assembly is used for positioning the inoculating needle and the position of the injection needle.

7. The system for preparing a microorganism mass spectrometry identification sample of claim 1, wherein: the culture dish placing area is provided with two oppositely arranged positioning stop blocks, and a culture dish placing space capable of containing the culture dish is formed between the two positioning stop blocks.

8. The system for preparing a microorganism mass spectrometry identification sample of claim 1, wherein: the target plate placing mechanism further comprises a target plate seat, a first groove capable of containing the target plate is formed in the top of the target plate seat, a second groove capable of being sleeved on the inner side positioning block is formed in the bottom of the target plate seat, and the first groove is communicated with the second groove.

9. The system for preparing a microorganism mass spectrometry identification sample of claim 1, wherein: the mobile module comprises a second mobile module and a third mobile module, the third mobile module is connected with the output end of the second mobile module, the bottom base is connected with the output end of the third mobile module, and a preset included angle is formed between the mobile direction of the second mobile module and the mobile direction of the third mobile module.

10. The system for preparing a microorganism mass spectrometry identification sample of claim 1, wherein: and a sterilization mechanism is arranged in the cavity.