CN113248324A - Biological organic fertilizer for reducing heavy metal cadmium absorbed by rice and preparation method thereof - Google Patents
Biological organic fertilizer for reducing heavy metal cadmium absorbed by rice and preparation method thereof Download PDFInfo
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- CN113248324A CN113248324A CN202110521563.5A CN202110521563A CN113248324A CN 113248324 A CN113248324 A CN 113248324A CN 202110521563 A CN202110521563 A CN 202110521563A CN 113248324 A CN113248324 A CN 113248324A
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 53
- 235000009566 rice Nutrition 0.000 title claims abstract description 53
- 229910052793 cadmium Inorganic materials 0.000 title claims abstract description 44
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 31
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title description 12
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 52
- 241001465754 Metazoa Species 0.000 claims abstract description 39
- 210000002381 plasma Anatomy 0.000 claims abstract description 35
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 32
- 238000000855 fermentation Methods 0.000 claims abstract description 24
- 230000004151 fermentation Effects 0.000 claims abstract description 24
- 238000010521 absorption reaction Methods 0.000 claims abstract description 22
- 102000004400 Aminopeptidases Human genes 0.000 claims abstract description 20
- 108090000915 Aminopeptidases Proteins 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 108090000145 Bacillolysin Proteins 0.000 claims abstract description 18
- 102000035092 Neutral proteases Human genes 0.000 claims abstract description 18
- 108091005507 Neutral proteases Proteins 0.000 claims abstract description 18
- 239000003146 anticoagulant agent Substances 0.000 claims abstract description 13
- 229940127219 anticoagulant drug Drugs 0.000 claims abstract description 13
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims abstract description 12
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000002509 fulvic acid Substances 0.000 claims abstract description 12
- 229940095100 fulvic acid Drugs 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 150000003751 zinc Chemical class 0.000 claims abstract description 11
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims abstract description 9
- 150000001638 boron Chemical class 0.000 claims abstract description 9
- 150000002505 iron Chemical class 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 31
- 238000002156 mixing Methods 0.000 claims description 24
- 239000003337 fertilizer Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- 230000003698 anagen phase Effects 0.000 claims description 3
- 102000057297 Pepsin A Human genes 0.000 claims description 2
- 108090000284 Pepsin A Proteins 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims description 2
- 229940111202 pepsin Drugs 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 25
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 102000004190 Enzymes Human genes 0.000 description 12
- 108090000790 Enzymes Proteins 0.000 description 12
- 229940088598 enzyme Drugs 0.000 description 12
- 238000003756 stirring Methods 0.000 description 12
- 108090000765 processed proteins & peptides Proteins 0.000 description 9
- 239000001509 sodium citrate Substances 0.000 description 9
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 9
- 239000002689 soil Substances 0.000 description 9
- 239000000378 calcium silicate Substances 0.000 description 8
- 229910052918 calcium silicate Inorganic materials 0.000 description 8
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 description 8
- 239000011790 ferrous sulphate Substances 0.000 description 8
- 235000003891 ferrous sulphate Nutrition 0.000 description 8
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical group [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 8
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 8
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical group [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 8
- 229960001763 zinc sulfate Drugs 0.000 description 8
- 229910000368 zinc sulfate Inorganic materials 0.000 description 8
- 229910021538 borax Inorganic materials 0.000 description 7
- 239000004328 sodium tetraborate Substances 0.000 description 7
- 235000010339 sodium tetraborate Nutrition 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 238000005086 pumping Methods 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 4
- -1 and more preferably Chemical class 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- 239000004021 humic acid Substances 0.000 description 4
- 229920001184 polypeptide Polymers 0.000 description 4
- 150000003376 silicon Chemical class 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000004115 Sodium Silicate Substances 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 description 3
- 229910000360 iron(III) sulfate Inorganic materials 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000003307 slaughter Methods 0.000 description 3
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical group [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 3
- 229910052911 sodium silicate Inorganic materials 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- LEZAYTDLNNEFJT-UHFFFAOYSA-N tetracosasodium octaborate tetrahydrate Chemical compound O.O.O.O.[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-] LEZAYTDLNNEFJT-UHFFFAOYSA-N 0.000 description 3
- WGIWBXUNRXCYRA-UHFFFAOYSA-H trizinc;2-hydroxypropane-1,2,3-tricarboxylate Chemical compound [Zn+2].[Zn+2].[Zn+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O WGIWBXUNRXCYRA-UHFFFAOYSA-H 0.000 description 3
- 239000011746 zinc citrate Substances 0.000 description 3
- 235000006076 zinc citrate Nutrition 0.000 description 3
- 229940068475 zinc citrate Drugs 0.000 description 3
- 244000299452 Gouania lupuloides Species 0.000 description 2
- 235000000292 Gouania lupuloides Nutrition 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 2
- 239000004327 boric acid Substances 0.000 description 2
- 235000012241 calcium silicate Nutrition 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000001450 anions Chemical group 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 159000000014 iron salts Chemical class 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 238000002161 passivation Methods 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000007198 pollen germination Effects 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- ZNCPFRVNHGOPAG-UHFFFAOYSA-L sodium oxalate Chemical compound [Na+].[Na+].[O-]C(=O)C([O-])=O ZNCPFRVNHGOPAG-UHFFFAOYSA-L 0.000 description 1
- 229940039790 sodium oxalate Drugs 0.000 description 1
- 239000002426 superphosphate Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/10—Addition or removal of substances other than water or air to or from the material during the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Soil Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Plant Pathology (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a biological organic fertilizer for reducing heavy metal cadmium absorbed by rice, and relates to the technical field of agricultural biology. A biological organic fertilizer for reducing heavy metal cadmium absorbed by rice comprises the following raw materials in parts by weight: 20-30 parts of animal blood plasma, 0.1-0.2 part of anticoagulant, 0.2-0.3 part of neutral protease, 0.02-0.04 part of aminopeptidase, 8-12 parts of fulvic acid, 6-8 parts of silicate, 1-3 parts of boron salt, 1-2 parts of iron salt, 1-2 parts of zinc salt, 8-12 parts of pseudomonas aeruginosa fermentation liquor and 60-80 parts of water. The invention has the following beneficial effects: (1) the invention develops animal plasma into a biological organic fertilizer for reducing the absorption of heavy metal cadmium by rice, realizes the development and reutilization of waste animal plasma in slaughterhouses, increases the bioavailability of the animal plasma and lightens the environmental pollution. (2) The rice yield is improved. (3) The effect of reducing cadmium in rice is realized.
Description
Technical Field
The invention relates to the technical field of agricultural biology, in particular to a biological organic fertilizer for reducing heavy metal cadmium absorbed by rice and a preparation method thereof.
Background
The consumption of meat is continuously increased worldwide, and the slaughtering quantity of livestock is continuously increased every year; the worldwide blood volume for slaughtering animals is increasing sharply to about 2,000 million tons per year, with an effective utilization rate of less than 10-20%, with the fact that most of the blood is discarded, the direct discharge of the blood pollutes water and soil, and the effective treatment requires a lot of capital.
With the increasing awareness of the environmental protection, more and more people and enterprises are aware of the value of animal slaughter blood. Animal blood is rich in proteins and nutrients, and there are many possibilities for reuse.
Therefore, providing a new product developed by using animal blood is a problem that needs to be solved by those skilled in the art.
Disclosure of Invention
In view of the above, the invention provides a biological organic fertilizer for reducing the absorption of heavy metal cadmium by rice.
In order to achieve the purpose, the invention adopts the following technical scheme:
a biological organic fertilizer for reducing heavy metal cadmium absorbed by rice comprises the following raw materials in parts by weight: 20-30 parts of animal blood plasma, 0.1-0.2 part of anticoagulant, 0.2-0.3 part of neutral protease, 0.02-0.04 part of aminopeptidase, 8-12 parts of fulvic acid, 6-8 parts of silicate, 1-3 parts of boron salt, 1-2 parts of iron salt, 1-2 parts of zinc salt, 8-12 parts of pseudomonas aeruginosa fermentation liquor and 60-80 parts of water.
Has the advantages that: the biological organic fertilizer for reducing the absorption of heavy metal cadmium by rice meets the nutritional requirements of soil and rice aiming at the current situation of nutrient elements in soil, has the effects of promoting the growth and tillering of rice, promoting the filling of rice grains and improving the maturing rate, and can realize the adsorption of cadmium in soil by the cooperation of pseudomonas aeruginosa, silicate and brown humic acid, thereby achieving the excellent effect of reducing cadmium in rice grains.
As a preferable technical scheme of the invention, the raw materials comprise the following components in parts by weight: 25 parts of animal blood plasma, 0.15 part of anticoagulant, 0.25 part of neutral protease, 0.03 part of aminopeptidase, 10 parts of fulvic acid, 7 parts of silicate, 2 parts of boron salt, 1.5 parts of ferric salt, 1.5 parts of zinc salt, 10 parts of pseudomonas aeruginosa fermentation liquor and 70 parts of water.
As a preferred technical proposal of the invention, the pseudomonas aeruginosa fermentation liquor is the fermentation liquor in the logarithmic growth phase of the pseudomonas aeruginosa, and the concentration of the pseudomonas aeruginosa is 1 multiplied by 108～1×109cfu/mL。
Has the advantages that: the pseudomonas aeruginosa has strong adsorption effect on cadmium in soil, and can effectively adsorb the cadmium due to electronegativity represented by the anion groups on the surface of the pseudomonas aeruginosa; in addition, the pseudomonas aeruginosa has huge specific surface area and outstanding adsorption capacity to cadmium, and can realize the synergistic fixation effect on cadmium with the silicate. The pseudomonas aeruginosa is in the logarithmic growth phase, the growth and reproduction capacity is strong, and the cadmium adsorption and fixation effect is better.
Preferably, the anticoagulant is one or more of sodium citrate, sodium oxalate and EDTA.
Preferably, the enzyme activity of the neutral protease is 5 ten thousand u/g; the enzyme activity of the aminopeptidase is 10 ten thousand u/g.
Has the advantages that: the neutral protease is endonuclease, and can carry out enzymolysis on animal plasma to degrade the animal plasma into mixed solution of polypeptide, short peptide and amino acid. The aminopeptidase is an exoenzyme, and can further degrade polypeptides and short peptides from an amino terminal to generate amino acid molecules. The degraded amino acid, polypeptide and short peptide are taken as nutrient substances to be absorbed by the root system or leaf blade of the rice on one hand, and a small part of short peptide can be taken as plant cell signal conduction to promote the division and tillering quantity of the rice cell on the other hand.
Preferably, the silicon salt is sodium silicate and/or calcium silicate, and more preferably, the silicon salt is calcium silicate.
Has the advantages that: the silicate has a passivation effect on cadmium in soil, and can fix the cadmium in the soil and prevent the cadmium from migrating to rice. After the rice absorbs the silicate, silicified cells are formed to improve the strength of the rice cell wall, the plant is upright, the light receiving situation is obviously changed, and ventilation and light transmission and accumulation of organic matters are facilitated; the formation of silicified cells also promotes the increase of the rice cuticle, forms a firm protective layer and improves the stress resistance of the rice.
Preferably, the boron salt is one or more of borax, boric acid and sodium octaborate tetrahydrate, and more preferably, the silicon salt is borax.
Has the advantages that: the boron salt is an essential element of rice, boron influences the efficiency of chloroplast and photosynthesis, can promote the operation of photosynthetic products, seed germination and seedling growth, can promote pollen germination and pollen tube growth, promotes pollination fertilization and improves the maturing rate.
Preferably, the iron salt is ferrous sulfate and/or ferric sulfate, and more preferably, the iron salt is ferrous sulfate.
Preferably, the zinc salt is zinc sulfate and/or zinc citrate, and more preferably, the zinc salt is zinc sulfate.
Has the advantages that: at present, the iron and zinc contents of soil in China are generally low, and the iron salt and zinc salt contents are added, so that the nutritional requirements of rice on iron salts and zinc salts in growth can be met, and the stress resistance of the rice is improved.
The invention also aims to provide a preparation method of the biological organic fertilizer for reducing the absorption of heavy metal cadmium by rice, which comprises the following steps:
S1：
adding an anticoagulant into fresh animal plasma, and uniformly mixing to obtain anticoagulant animal plasma;
adding water and neutral protease into the anticoagulated animal plasma, and carrying out enzymolysis to obtain an enzymolysis solution A;
taking 20-50 wt% of enzymolysis liquid A, adding aminopeptidase for enzymolysis to obtain enzymolysis liquid B;
mixing the enzymolysis liquid B with the residual enzymolysis liquid A to obtain enzymolysis liquid C;
S2：
and adding the fulvic acid, the silicon salt, the boron salt, the ferric salt, the zinc salt and the pseudomonas aeruginosa fermentation liquor into the enzymolysis liquid C, and uniformly mixing to obtain the biological organic fertilizer for reducing the absorption of heavy metal cadmium by the rice.
Has the advantages that: the invention carries out fractional enzymolysis after the anticoagulation treatment of fresh animal plasma, firstly degrades all the animal plasma by neutral protease to generate mixed solution of polypeptide, short peptide and amino acid, and then further degrades part of the mixed solution (enzymolysis solution A) by aminopeptidase to generate amino acid, which is beneficial to the absorption and utilization of plants.
As the preferable technical scheme of the invention, the enzymolysis temperature of neutral pepsin is 55-60 ℃, and the enzymolysis time is 4-5 hours; the enzymolysis temperature of the aminopeptidase is 35-40 ℃, and the enzymolysis time is 3-4 hours.
As a preferable technical scheme of the invention, the mixing speed of the step S1 is 50-100 rpm, and the time is 2-5 min; in the step S2, the mixing speed is 20-30 rpm, and the mixing time is 10-20 min.
The invention also aims to provide the application of the biological organic fertilizer for reducing the absorption of heavy metal cadmium by rice in the monocotyledon crop planting.
As the preferred technical scheme of the invention, the biological organic fertilizer for reducing the absorption of heavy metal cadmium by rice can be sprayed on leaf surfaces and/or applied along with water.
A more preferred embodiment is the application with water.
According to the technical scheme, the invention discloses and provides the biological organic fertilizer for reducing the absorption of heavy metal cadmium by rice and the preparation method and application thereof, and compared with the prior art, the biological organic fertilizer has the following beneficial effects:
(1) the invention develops animal plasma into a biological organic fertilizer for reducing the absorption of heavy metal cadmium by rice, realizes the development and reutilization of waste animal plasma in slaughterhouses, increases the bioavailability of the animal plasma and lightens the environmental pollution.
(2) The rice yield is improved.
(3) The effect of reducing cadmium in rice is realized.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention discloses a biological organic fertilizer for reducing heavy metal cadmium absorbed by rice and a preparation method and application thereof. The raw materials involved in the present invention are all commercially available, and the sources thereof are not particularly limited. The methods used in the present invention are conventional methods unless otherwise specified, and are not described herein.
Example 1
The raw material formula is as follows:
25 parts of animal blood plasma, 0.15 part of sodium citrate, 0.25 part of neutral protease (the enzyme activity is 5 wu/g), 0.03 part of aminopeptidase (the enzyme activity is 10 wu/g), 10 parts of brown humic acid, 7 parts of calcium silicate, 2 parts of borax, 1.5 parts of ferrous sulfate, 1.5 parts of zinc sulfate, 10 parts of pseudomonas aeruginosa fermentation liquor and 70 parts of water, wherein the concentration of the pseudomonas aeruginosa fermentation liquor is 1.5 multiplied by 108cfu/mL。
The preparation method comprises the following steps:
(1) adding sodium citrate into fresh animal plasma, stirring at the speed of 50-100 rpm for 2-5 min, and uniformly mixing to obtain anticoagulant animal plasma;
adding water and neutral protease into anticoagulated animal plasma, uniformly stirring, and carrying out enzymolysis for 4.5h at 55-60 ℃ to obtain an enzymolysis liquid A;
pumping 60 wt% of enzymolysis liquid A into a blending tank, adding aminopeptidase into the remaining 40 wt% of enzymolysis liquid A, uniformly stirring, and carrying out enzymolysis for 4 hours at 35-40 ℃ to obtain enzymolysis liquid B;
pumping the enzymolysis liquid B into a blending tank to obtain enzymolysis liquid C;
(2) and (3) adding fulvic acid, calcium silicate, borax, ferrous sulfate, zinc sulfate and the pseudomonas aeruginosa fermentation liquor in the logarithmic phase into the enzymolysis solution C respectively, stirring at the speed of 20-30 rpm for 10-20 min, and uniformly mixing to obtain the biological organic fertilizer for reducing the absorption of heavy metal cadmium by rice.
Example 2
The raw material formula is as follows:
20 parts of animal blood plasma, 0.1 part of sodium citrate, 0.2 part of neutral protease (the enzyme activity is 5 wu/g), 0.02 part of aminopeptidase (the enzyme activity is 10 wu/g), 8 parts of fulvic acid, 6 parts of calcium silicate, 1 part of boric acid, 1 part of ferrous sulfate, 1 part of zinc sulfate, 8 parts of pseudomonas aeruginosa fermentation liquor and 60 parts of water, wherein the concentration of the pseudomonas aeruginosa fermentation liquor is 1.3 multiplied by 108cfu/mL。
The preparation method comprises the following steps:
(1) adding sodium citrate into fresh animal plasma, stirring at the speed of 50-100 rpm for 2-5 min, and uniformly mixing to obtain anticoagulant animal plasma;
adding water and neutral protease into anticoagulated animal plasma, uniformly stirring, and carrying out enzymolysis for 4 hours at 55-60 ℃ to obtain an enzymolysis liquid A;
pumping 80 wt% of enzymolysis liquid A into a blending tank, adding aminopeptidase into the remaining 20 wt% of enzymolysis liquid A, uniformly stirring, and carrying out enzymolysis for 3 hours at 35-40 ℃ to obtain enzymolysis liquid B;
pumping the enzymolysis liquid B into a blending tank to obtain enzymolysis liquid C;
(2) and (3) adding fulvic acid, calcium silicate, borax, ferrous sulfate, zinc sulfate and the pseudomonas aeruginosa fermentation liquor in the logarithmic phase into the enzymolysis solution C respectively, stirring at the speed of 20-30 rpm for 10-20 min, and uniformly mixing to obtain the biological organic fertilizer for reducing the absorption of heavy metal cadmium by rice.
Example 3
The raw material formula is as follows:
30 parts of animal plasma, 0.2 part of sodium citrate, 0.3 part of neutral protease (the enzyme activity is 5 wumu/g), 0.04 part of aminopeptidase (the enzyme activity is 10 wumu/g), 12 parts of brown humic acid, 8 parts of sodium silicate, 3 parts of sodium octaborate tetrahydrate, 2 parts of ferric sulfate, 2 parts of zinc citrate, 12 parts of pseudomonas aeruginosa fermentation liquor and 80 parts of water, wherein the concentration of the pseudomonas aeruginosa fermentation liquor is 1.7 multiplied by 108cfu/mL。
The preparation method comprises the following steps:
(1) adding sodium citrate into fresh animal plasma, stirring at the speed of 50-100 rpm for 2-5 min, and uniformly mixing to obtain anticoagulant animal plasma;
adding water and neutral protease into anticoagulated animal plasma, uniformly stirring, and carrying out enzymolysis for 5 hours at 55-60 ℃ to obtain an enzymolysis liquid A;
pumping 50 wt% of the enzymolysis liquid A into a blending tank, adding aminopeptidase into the remaining 50 wt% of the enzymolysis liquid A, uniformly stirring, and carrying out enzymolysis for 4 hours at 35-40 ℃ to obtain enzymolysis liquid B;
pumping the enzymolysis liquid B into a blending tank to obtain enzymolysis liquid C;
(2) and (3) adding the fulvic acid, sodium silicate, sodium octaborate tetrahydrate, ferric sulfate, zinc citrate and the pseudomonas aeruginosa fermentation liquor in the logarithmic phase into the enzymolysis liquid C respectively, stirring at the speed of 20-30 rpm for 10-20 min, and uniformly mixing to obtain the biological organic fertilizer for reducing the absorption of heavy metal cadmium by rice.
Comparative example 1 (adjustment of amount of silicon salt)
The raw material formula is as follows:
25 parts of animal blood plasma, 0.15 part of sodium citrate, 0.25 part of neutral protease (the enzyme activity is 5 wu/g), 0.03 part of aminopeptidase (the enzyme activity is 10 wu/g), 10 parts of fulvic acid, 1 part of calcium silicate, 2 parts of borax, 1.5 parts of ferrous sulfate, 1.5 parts of zinc sulfate, 10 parts of pseudomonas aeruginosa fermentation liquor and 70 parts of water, wherein the concentration of the pseudomonas aeruginosa fermentation liquor is 1.6 multiplied by 108cfu/mL。
The preparation method is the same as example 1.
Comparative example 2 (adjusting the amount of Pseudomonas aeruginosa broth)
The raw material formula is as follows:
25 parts of animal blood plasma, 0.15 part of sodium citrate, 0.25 part of neutral protease (the enzyme activity is 5 wu/g), 0.03 part of aminopeptidase (the enzyme activity is 10 wu/g), 10 parts of brown humic acid, 7 parts of calcium silicate, 2 parts of borax, 1.5 parts of ferrous sulfate, 1.5 parts of zinc sulfate, 1 part of pseudomonas aeruginosa fermentation liquor and 70 parts of water, wherein the concentration of the pseudomonas aeruginosa fermentation liquor is 1.6 multiplied by 108cfu/mL。
The preparation method is the same as example 1.
Effect example 1
Test site:
the south of the west village, the west, the right, of the north gate town of the south area of Tianjin, 38 degrees at the north latitude, 56 '10 degrees at the north latitude, and 117 degrees at the east longitude, 22' 0 degrees at the east longitude.
A sowing mode:
soil preparation and fertilization are carried out in 25 days in 4 months and in 30 days in 4 months in 2019 (rice variety Jinyuan E28), and the seeding rate is 23.5kg/667m2The row spacing is 30cm, the plant spacing is 18cm, and the number of holes per mu is 1.23 ten thousand.
Fertilizer to be tested:
biological organic fertilizers obtained in examples 1 to 3 and comparative examples 1 to 2, and conventional chemical fertilizer [ urea (N: 46%), triple superphosphate (P) in comparative example 32O550 percent of potassium sulfate (K)2O:50％)]。
A fertilizing mode: the fertilizing amount is shown in table 1 in terms of mu.
TABLE 1
And (3) test results:
and (4) observing the fixed-point plant fixing every three days from 6 months and 20 days, and mainly investigating seedling height, root length, white root number, fresh weight, dry weight, heavy metal element content and the like. The harvest time was tested for yield elements and the yield was measured by single harvest and the results are shown in table 2.
TABLE 2
Note: the cadmium content of the rice is entrusted to the quality supervision and inspection test center of the rice and products in rural areas of agriculture for detection according to GB 5009.268-2016.
As can be seen from the results in Table 2, the application of the bio-organic fertilizer for reducing the absorption of heavy metal cadmium by rice can significantly improve the yield of rice and reduce the content of cadmium in rice. Wherein, compared with the conventional chemical fertilizer control (comparative example 3), the yield of the example 1 is increased by 275.5 kg/mu, and the yield is increased by 70.6 percent; the cadmium content in the rice is reduced by 0.172 mg/kg.
Effect example 2
Mode of administration
Test site: (same effect example 1 land)
A sowing mode: (same effect example 1)
Fertilizer to be tested: the bioorganic fertilizer obtained in example 1.
The application mode is as follows: the fertilizing amount is shown in table 3.
TABLE 3
And (3) test results:
and (4) observing the fixed-point plant fixing every three days from 6 months and 20 days, and mainly investigating seedling height, root length, white root number, fresh weight, dry weight, heavy metal element content and the like. The harvest time was tested for yield elements and the yield was measured by single harvest and the results are shown in table 4.
TABLE 4
As can be seen from the results in Table 4, the bio-organic fertilizer for reducing the absorption of heavy metal cadmium by rice of the invention has the best effect along with the water fertilization.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. A biological organic fertilizer for reducing heavy metal cadmium absorbed by rice is characterized by comprising the following raw materials in parts by weight: 20-30 parts of animal blood plasma, 0.1-0.2 part of anticoagulant, 0.2-0.3 part of neutral protease, 0.02-0.04 part of aminopeptidase, 8-12 parts of fulvic acid, 6-8 parts of silicate, 1-3 parts of boron salt, 1-2 parts of iron salt, 1-2 parts of zinc salt, 8-12 parts of pseudomonas aeruginosa fermentation liquor and 60-80 parts of water.
2. The biological organic fertilizer for reducing heavy metal cadmium absorption of rice as claimed in claim 1, wherein the raw materials comprise, by weight: 25 parts of animal blood plasma, 0.15 part of anticoagulant, 0.25 part of neutral protease, 0.03 part of aminopeptidase, 10 parts of fulvic acid, 7 parts of silicate, 2 parts of boron salt, 1.5 parts of ferric salt, 1.5 parts of zinc salt, 10 parts of pseudomonas aeruginosa fermentation liquor and 70 parts of water.
3. The biological organic fertilizer for reducing absorption of heavy metal cadmium by rice as claimed in claim 1, wherein the pseudomonas aeruginosa fermentation liquor is fermentation liquor in logarithmic growth phase of pseudomonas aeruginosa, and the concentration of pseudomonas aeruginosa is 1 x 108～1×109cfu/mL。
4. The method for preparing the biological organic fertilizer for reducing the absorption of the heavy metal cadmium by the rice as claimed in any one of claims 1 to 3, which is characterized by comprising the following steps:
S1：
adding an anticoagulant into fresh animal plasma, and uniformly mixing to obtain anticoagulant animal plasma;
adding water and neutral protease into the anticoagulated animal plasma, and carrying out enzymolysis to obtain an enzymolysis solution A;
taking 20-50 wt% of enzymolysis liquid A, adding aminopeptidase for enzymolysis to obtain enzymolysis liquid B;
mixing the enzymolysis liquid B with the residual enzymolysis liquid A to obtain enzymolysis liquid C;
S2：
and adding the fulvic acid, the silicon salt, the boron salt, the ferric salt, the zinc salt and the pseudomonas aeruginosa fermentation liquor into the enzymolysis liquid C, and uniformly mixing to obtain the biological organic fertilizer for reducing the absorption of heavy metal cadmium by the rice.
5. The method for preparing the biological organic fertilizer for reducing the absorption of the heavy metal cadmium by the rice according to claim 4, wherein the neutral pepsin enzymolysis temperature is 55-60 ℃, and the enzymolysis time is 4-5 hours; the enzymolysis temperature of the aminopeptidase is 35-40 ℃, and the enzymolysis time is 3-4 hours.
6. The method for preparing the bio-organic fertilizer for reducing the absorption of heavy metal cadmium by rice according to claim 4, wherein the mixing speed of the step S1 is 50-100 rpm, and the time is 2-5 min; in the step S2, the mixing speed is 20-30 rpm, and the mixing time is 10-20 min.
7. The use of the bioorganic fertilizer for reducing the absorption of heavy metal cadmium by rice as claimed in any one of claims 1 to 3 in monocotyledonous crop planting.
8. The use according to claim 7, characterized in that the bioorganic fertilizer is used as foliar spray and/or with water application.
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