CN113229223A - Reproduction method of centipedes hastellae - Google Patents

Abstract

The invention belongs to the technical field of medicinal animal feeding, and particularly relates to a centipede breeding method, which is simple to operate, simple and easy to operate in breeding steps, and easy to obtain breeding tools, so that the breeding cost is saved, the breeding period can be effectively shortened, the survival rate and the sub-algebra quantity of centipedes can be obviously improved, and the fatality rate is reduced; meanwhile, the centipede hastelling bred by the method has excellent offspring biological indexes, more consistent growth vigor, active and robust individuals and good quality, can meet the primary standard requirements of traditional Chinese medicinal materials, can provide stable and reliable animal materials for scientific research and medicine on the one hand, is favorable for carrying out large-scale ecological breeding of medicinal animal centipedes in subsequent industrial pharmacy, and is favorable for sustainable development of the industrial chain related to the medicinal animal centipedes on the other hand.

Description

Reproduction method of centipedes hastellae

Technical Field

The invention belongs to the technical field of medicinal animal feeding, and particularly relates to a centipede hastelling breeding method.

Background

Centipedes, which are ancient members of terrestrial invertebrates and widely distributed in the world, are specialized as jaw limbs for first step feet, can secrete venom, and are mainly used for predation and defense. Centipede has been used as an animal medicine in China for more than two thousand years. As early as in Shen nong Ben Cao Jing, there is a record about the medication of centipedes, and the pharmacological actions of centipedes are classified as toxic, sweet and mild in flavor and entering the stomach meridian. The centipede animal medicine for Chinese medicine is prepared with centipede (Scolopendendrasubspinos mutilans L. Koch) as well as 6 species including Khaki centipede, ink river centipede, multi-thorn centipede, etc. all of which are used as medicinal centipedes in different areas of China.

Scolopendra, a traditional Chinese medicine, has effects of counteracting toxic substances, killing parasites, dispelling pathogenic wind and relieving pain, and is commonly used for treating pyocutaneous disease, pyogenic infection, acute mastitis, disease, intractable tinea, toothache, rheumatism, arthralgia, etc. Modern pharmacological researches find that main drug-effect substances of the centipedes comprise proteins, enzymes, fatty acids and the like, and have the effects of regulating lipid metabolism, reducing blood fat, enhancing coronary blood flow, improving immunity, resisting aging, tranquilizing and easing pain and the like. Modern medical research finds that centipedes can be used for treating various diseases, such as partial cancers, and centipede water extract has a strong inhibiting effect on ovarian cancer, liver cancer, lung cancer, gastric cancer, colon cancer and other cancer cells; and can be used for treating apoplexy, hemiplegia, infantile convulsion, etc., and male diseases with good therapeutic effect.

The centipede venom has more complex components and different species of centipede venom have different pharmacological properties. Moreover, compared with other toxic animal species, the centipede venom has more remarkable difference in components, which is also a material basis for long-standing medicine of important animals. After the venom of the centipede hastelling is purified, the venom protein or polypeptide substances of the centipede hastelling have various pharmacological activities different from other centipede species, such as trypsin inhibitory activity, platelet aggregation activity, voltage-gated potassium channel activity and the like. The pharmacological action of the differences also enables the centipede hastelling to have better advantages in the aspects of biological indexes and pharmacology.

Kyllinga cantonensis (Scolopendra subspinipes dehaani Brandt) belongs to the family Centipedes, is a centipede with large individual and large venom amount, generally has an individual body length of 7-20cm, and the average body length of adults fed in Xishuangbanna can reach 22 cm. The centipedes hastelling are distributed in subtropical areas of tropical zones such as Yunnan, Hainan, Guangdong and Guangxi, but the quantity is small; investigation finds that the centipede Hardgrove is the dominant species in the West-Shuangbanna area of Yunnan. In Xishuangbanna, the villous amomum species under rubber forest are distributed with more centipedes, which can prey on mollusks (such as snails) and various pests (such as locusts, grasshoppers, crickets, cockchafers and the like) due to the complex feeding habits. The centipede hastella is diversified in application value, and from the perspective of traditional Chinese medicinal materials, the centipede hastella can be used as an important animal medicine resource; from the perspective of biological control of agricultural pests, the centipede hastelling can be further developed and utilized as the dominant natural enemy of rubber and fructus amomi planting areas, namely the centipede hastelling is utilized as a control agent of natural enemy organisms, and the pests are controlled by the principle of controlling the pests by the pests.

The centipede hastelling is one of the traditional Chinese medicinal materials in China and has good medicinal value. However, wild resources of the centipede hastelling are very limited at present, and the requirements of the medicinal market can not be met. Although the number of centipedes can be improved to a certain extent by artificial feeding, no report related to the artificial feeding technology of centipedes hastella is available so far. Due to the lack of key biological information and feeding technology for feeding the centipedes hasteng, the centipedes hasteng in the current market have different quality and are difficult to reach the standard of excellent centipedes, so that the standardized ecological process of the centipedes is blocked, and the safety and the effectiveness of clinical medication are difficult to ensure. Therefore, an efficient and scientific centipede breeding method is needed to be developed to improve the breeding quality of centipedes hastelling and promote the standardized and large-scale breeding process of the centipedes hastelling to meet the standard of standard medicine use. The patent researches the breeding technology of the centipede hastelling for the first time, and has important significance for further development and utilization of the centipede hastelling.

Disclosure of Invention

The invention provides a breeding method of centipedes hastelling for the first time, the method is simple to operate, tools for breeding are easy to obtain, the breeding cost is saved, the survival rate of centipedes is obviously improved, compared with the field environment, the period of application of centipedes in medicine application is greatly shortened, the raw material cost of the centipedes is reduced, a large number of centipedes hastelling with consistent quality and excellent quality can be produced in a large scale in a short time and supplied to the market as traditional Chinese medicinal materials, and medicinal centipede raw materials with controllable quality are provided.

In order to achieve the purpose, the invention adopts the technical scheme that:

a reproduction method of centipedes hastelling comprises the following steps:

s1, culturing the centipedes with the climatic chamber to the oviposition stage, picking out the female centipedes with the oviposition stage after one week of oviposition, controlling the temperature in the climatic chamber to be 18-34 ℃, the humidity to be 60-80% and the light cycle to be dark during the culturing period: illumination 12: 12;

s2, transferring the picked brooding Khattai centipedes to feeding boxes for independent feeding, placing the feeding boxes in a climatic box, and paving 1.5-2.5 cm-high padding in the feeding boxes, wherein the padding is prepared from coconut chaff and brick red soil according to the ratio of 7: (2-4), adding 15-25mL of traditional Chinese medicine liquid into the padding, and adjusting the humidity of the padding to be 20% -28% by using water, wherein the traditional Chinese medicine liquid is prepared from Dai Baijie and kidney tea;

s3, transferring the brood Kjellyfish centipedes from the feeding box to a climatic box to be fed separately from the larva after the brood Kjellyfish centipedes are hatched, transferring the brood Kjellyfish centipedes from the feeding box to the climatic box to be fed when the brood centipedes can move independently, and feeding the brood centipedes to an adult;

during the raising period of the steps S1 to S3, 7-9g of feed is fed to the centipedes every time in the non-oviposition period, the feed fed every time in the oviposition period is increased to 10-12g, the centipedes are not fed in the hatching period, the centipedes are observed and fed once every 3 days, and the centipedes are fed at 20: 00, and feeding feed comprises living animals and common meat.

The established breeding method of the centipede hastelling can accurately and simply carry out propagation, and provide the centipede Chinese medicinal materials which have excellent biological indexes, more consistent growth vigor, active and robust individuals and good quality and can meet the first-level standard requirements of the Chinese medicinal materials. Is beneficial to large-scale ecological breeding of the centipedes hastelling, is beneficial to promoting the sustainable development of related industrial chains, and is beneficial to promoting the rural happy strategy practice taking traditional Chinese medicine breeding as a gripper. Therefore, the biological characteristics of the centipedes susceptible to fungal diseases are combined, the feeding boxes are adopted for feeding separately in the incubation period and the larva growth period, diversified living animal characteristic feeds are fed in the feeding period, and the characteristic Dai medicine is adopted as an additive component of the mixture padding, so that the cross infection of diseases can be effectively prevented, the centipedes hasten are enabled to be good in performance on various morphological indexes, the centipedes are enabled to be more robust, and the breeding of good varieties is facilitated; the centipede medicinal parts are the whole centipede, the centipede hastelling offspring bred by the breeding method has excellent biological indexes, more consistent growth vigor, active and robust individuals, 80 larvae can be produced by each female, the average length of the adult is 22.1cm, the quality is good, and the centipede hastelling offspring can meet the first-level standard requirements of traditional Chinese medicinal materials. The yield and the quality of the centipede medicinal materials are obviously improved, and the cost such as the time period is effectively reduced.

The method provided by the invention has important significance for breeding and standardized large-scale feeding of the centipedes hastelling, gradually meeting the resource requirements of domestic medicine enterprises, promoting the ordered development of related industrial chains of the centipede traditional Chinese medicines and solving the market requirements of the domestic centipede traditional Chinese medicines.

The survival rate of the centipedes hastelling can be effectively improved by adopting the optimal temperature illumination humidity condition. Preferably, the temperature of the climatic chamber is 22-30 ℃.

Preferably, the preparation method of the traditional Chinese medicine liquid comprises the following steps: mixing Daibaijie and herba Clerodendranthi Spicati at a weight ratio of 1:2, boiling in water, decocting to one fourth of the original volume, and filtering the residue to obtain the Chinese medicinal liquid. Further, the feed-liquid ratio of the Daibeibaijie to water is 3 g: 400 mL.

Preferably, in the feed for feeding, the mass ratio of the living animals to the common meat is 1: 1.

Further, the living animals are any one of crickets, locusts, grasshoppers, yellow mealworms and snails, the common meat is any one of fresh chicken, chicken livers and pig livers, and the living animals and the common meat are matched at will and are fed and changed with different matching combinations each time.

Preferably, the bedding is prepared from coconut coir and red loam according to the weight ratio of 7: 3 in a weight ratio.

Preferably, the laying height of the padding is 2.0 cm.

Preferably, 30-60 centipedes are raised in each climatic chamber.

Preferably, 20-40 rearing boxes are placed per climatic chamber.

Preferably, during the rearing in steps S1 to S3, the climate box is kept quiet without excessive disturbance except for daily rearing management.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides a breeding method of centipedes hastelling, which is simple to operate, simple and easy to operate in breeding steps, easy to obtain breeding tools, not only saves the breeding cost, but also can effectively shorten the breeding period, obviously improve the survival rate and the sub-generation quantity of the centipedes hastelling and reduce the fatality rate; meanwhile, the centipede hastelling bred by the method has excellent offspring biological indexes, more consistent growth vigor, active and robust individuals and good quality, can meet the primary standard requirements of traditional Chinese medicinal materials, can provide stable and reliable animal materials for scientific research and medicine on the one hand, is favorable for carrying out large-scale ecological breeding of medicinal animal centipedes in subsequent industrial pharmacy, and is favorable for sustainable development of the industrial chain related to the medicinal animal centipedes on the other hand.

Drawings

FIG. 1 is a climatic chamber for propagation;

FIG. 2 shows a breeding box;

FIG. 3 is a view of Khatherum centipede raised in a climatic chamber;

FIG. 4 is a partial Khatherum centipede in a rearing box;

fig. 5 is an oviferous female centipede hastella.

Detailed Description

The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.

The experimental procedures in the following examples were carried out by conventional methods unless otherwise specified, and the test materials used in the following examples were commercially available by conventional methods unless otherwise specified.

Example 1 establishment of Khattacea harzianum reproduction method

(1) Early preparation:

1) the artificial propagation tools such as a climatic box (purchased from Ningbo Saifu laboratory instruments Co., Ltd., an intelligent artificial climatic box with the model of PRX-250B), a feeding box (with the length multiplied by the width multiplied by the height multiplied by 19cm multiplied by 12.5cm multiplied by 7.5cm, 10-15 small holes with the diameter of 2mm are respectively arranged on two long sides, purchased from Huangyan palm spider pet products Co., Ltd., Taizhou city) and tweezers need to be disinfected by 75% alcohol before use.

2) Preparation of traditional Chinese medicine liquid for padding (taking 500mL as an example): the traditional Chinese medicine liquid is prepared from Dai medicine, the Dai medicine comprises Dai Baijie (Marsdenia tenacissima) and Clerodendranthus spicatus (Cleriodendanthus spicatus), 15g of the Dai Baijie and 30g of the Clerodendranthus spicatus are respectively weighed, 2000mL of water is added after the Dai Baijie and the Clerodendranthus spicatus are uniformly mixed, the mixture is boiled to 500mL, medicine residues are filtered, the traditional Chinese medicine liquid is prepared, and the traditional Chinese medicine liquid is placed in an ice box for standby after being cooled at normal temperature.

3) Feed for feeding: comprises a living animal and common meat, wherein the mass ratio of the living animal to the common meat is 1: 1; the living animals are any one of crickets, locusts, grasshoppers, yellow mealworms and snails, the common meat is any one of fresh chicken, chicken livers and pig livers, and the living animals and the common meat are matched at will and are fed and changed with different matching combinations each time. For example, the first feeding is carried out by matching and combining cricket and pork liver, the second feeding is changed into locust and chicken, and the third feeding is changed into grasshopper and chicken liver … …, namely, the components matched in two adjacent times are different, and the rest is done by analogy.

(2) The specific propagation process comprises the following steps:

1) taking a climatic chamber as a feeding chamber (as shown in figure 1), culturing the centipedes with Ha's style to an oviposition stage, picking out female centipedes at the oviposition stage after one week of oviposition, feeding 30-60 (male and female centipedes in mixed culture) in each climatic chamber, controlling the temperature in the climatic chamber to be 18-34 ℃, the humidity to be 60-80% and the light cycle to be dark: illumination 12: 12, ensuring that the climate box is in a feeding room with good natural ventilation;

2) respectively transferring the picked oviparous hastella centipedes to feeding boxes (as shown in figure 2) by using tweezers for independent feeding, placing the feeding boxes in climatic boxes, placing 20-40 feeding boxes in each climatic box, and paving 1.5-2.5 cm-high padding in each feeding box, wherein the padding is prepared from coconut chaff and brick red soil according to the ratio of 7: (2-4), adding 15-25mL of traditional Chinese medicine liquid into the padding, and adjusting the humidity of the padding to be 20% -28% by using sterile water;

3) after brooding Khattacharbe is hatched into young Khattacharbe, the female Khattacharbe is transferred from the feeding box to the climate box by using tweezers to be fed separately from the larva, the young Khattacharbe is transferred from the feeding box to the climate box when being cultured to the stage of independent activity, and the feeding is finished after the young Khattacharbe is fed to the adult.

During the feeding period of each feeding step, keeping the climate box quiet, not interfering too much except daily feeding management, feeding 7-9g of feed for each time in a non-oviposit period, properly increasing the amount of the feed during the oviposit period, increasing the amount of the feed for each time to 10-12g, not feeding the centipedes (feeding the centipedes according to normal standard after the centipedes are hatched out of larvae), observing and feeding once every 3 days, and feeding 20: 00 is carried out.

Example 2 research on growth and development indexes of centipede Hardgrove at different development stages

The centipede hastelling is artificially fed according to the centipede hastelling propagation method in the embodiment 1, and the feeding process is briefly introduced as follows:

(1) culturing the Khatherum centipede in a climatic chamber until the Khatherum centipede is in an oviposition stage (the Khatherum centipede cultured in the climatic chamber is shown in figure 3; the female Khatherum centipede in the oviposition is shown in figure 5), wherein the temperature in the climatic chamber is 26 +/-1 ℃, the humidity is 70 +/-5%, and the light cycle is dark: illumination 12: 12; then picking 20 female centipedes which have spawned in the same batch;

(2) feeding 20 female centipedes with eggs in a feeding box (the Khattachar in the feeding box is shown in figure 4), wherein a layer of padding with the thickness of 2cm (the weight ratio of coconut chaff to brick red soil is 7: 3) is laid in the box, 20mL of Chinese medicinal liquid is added into the padding, and water is added to adjust the humidity to 24%;

keeping a climate box quiet during feeding, keeping the climate box quiet except daily feeding management without excessive interference, feeding the centipedes by about 8g each time in a non-oviposition period (the first feeding is crickets: pork liver 1:1), properly increasing the amount of the feeding during the oviposition period, increasing the amount of the feeding to about 11g each time, not feeding the centipedes in an incubation period (feeding according to normal standard after the centipedes are incubated out of larvae), observing and feeding every 3 days, and feeding the centipedes 20: 00 is carried out;

(3) after hatching the young centipedes, the brooding centipedes are transferred to a climatic chamber to be fed separately from the young centipedes, and the young centipedes are transferred to the climatic chamber to be fed when being cultured until the centipedes can move independently (about 45 days) until being fed to an adult (about one year).

In the raising process, growth and development indexes of offspring eggs, larvae, sub-adults and adults in each development stage are respectively observed, 20 centipedes are observed in each treatment group, observation is carried out for 2-3 times every week, morphological characteristics of each development stage are observed by using a stereoscopic microscope, and data such as the body length of the centipedes are measured by using a ruler.

As can be seen from Table 1, the centipedes Harris bred by the centipede breeding method of example 1 have an average adult length of 22.1 cm. According to the reported standards of centipede medicinal materials (refer to the literature 'Huoshayi, and the like, research on the commodity specification grade standard of centipede medicinal materials [ J ]. Anhui agricultural science, 2019,47 (16): 209-wall 211, 253', and specifically see the content of the commodity specification grade division of centipedes in the table 5 in the literature), the first-level standard is that the body length is more than or equal to 14cm, so that the centipedes hasteng fed by the method can reach the standard of the first-level medicinal materials.

TABLE 1 biological observations of the different developmental stages of Khatherum centipede

Note: data in the table are mean ± sem.

EXAMPLE 3 Effect of different temperatures on the growth and development of Khatherum

Culturing the centipedes hastelling by using a climatic chamber to the oviposition stage, selecting the female centipedes with the same batch of oviposition for testing, artificially feeding the centipedes hastelling according to the breeding method of the centipedes hastelling in the embodiment 1, observing 20 centipedes in each treatment group for 2-3 times in each week, wherein the feeding specific parameters are the same as those in the embodiment 2, and simultaneously, the climatic chamber temperature of each treatment group is respectively set to be 18 ℃, 22 ℃, 26 ℃, 30 ℃ and 34 ℃; the development stages of the offspring at each development stage were observed, and the stage (unit d) and death at each development stage were recorded for 5 temperature treatments.

As can be seen from Table 2, the overall trend of the development history of the Khatherum centipede is gradually shortened along with the increase of the temperature, wherein the mortality rates of the centipedes treated at 18 ℃ and 34 ℃ are obviously higher than those of the centipedes treated at 22 ℃, 26 ℃ and 30 ℃, and the mortality rates of the centipedes treated at 22-30 ℃ can be 0 in all development stages, so that the temperature of 22-30 ℃ is the optimum feeding temperature.

TABLE 2 duration of various developmental stages of Khatherum centipede at different temperatures

Note: data in the table are mean ± sem.

Example 4 study of the propagation duration of Khattacea

Culturing the centipedes hastelling by a climatic chamber to the oviposition stage, selecting the female centipedes with eggs of the same batch for testing, dividing the centipedes into two equal parts with 20 heads each, wherein one part is artificially fed according to the breeding method of the centipedes hastelling in the embodiment 1, the specific feeding parameters are the same as those in the embodiment 2, and the temperature of the climatic chamber is 26 ℃. The observation is performed 2-3 times per week, and the development period of each development stage of ovum, larva, sub-adult and adult is recorded.

And the other part is placed in a rubber forest planting area of the Dai autonomous State of the Xishuangbanna, Yunnan province for field propagation, investigation is carried out once every half month, system investigation is continuously carried out, and development periods of development stages of eggs, larvae, sub-adults and adults are observed and recorded.

The development period of indoor feeding is shown in table 2, the development time of the centipede hastelling from an egg to an adult is 348.5 days; the development period of the field propagation is shown in Table 3, the development time of the Khatherum harsleyanum from egg to adult is 592.4 d; compared with the field, the development period of indoor feeding is accelerated by 243.9 d. Therefore, the centipede fed by the method can obviously shorten the development period of the centipede and improve the breeding efficiency of the centipede.

TABLE 3 growth history of wild centipedes

Stage of development	History of life	Development calendar period (d)
			Egg	From first month of 1 to first month of 2	17.5±3.2
Development of larvae to independent activities	From 1 late ten days to 2 late ten days	35.1±4.8
			From larval to sub-adult	Last ten days of 3 months to middle ten days of June	425.4±24.7
From juvenile to adult	7 months and early in the next year	574.9±26.3

Note: data in the table are mean ± sem.

Example 5 Khatag Centipede 1 generation quantity study

After culturing the centipedes hastelling by using a climatic chamber to an oviposition stage, selecting 15 female centipedes which oviposit in the same batch for testing, and artificially feeding the centipedes hastelling according to the breeding method of the embodiment 1, wherein the specific feeding parameters are the same as those of the embodiment 2. The number of offspring (F1) was recorded at 2-3 times per week. Meanwhile, 3 female centipedes hastelling eggs are collected and observed in the field as field control, and the number of offspring (F1 generation) is observed.

As shown in the statistical results of the F1 generations of Khattachar centipedes in Table 4, the average number of the F1 generations of Khattachar centipedes bred by the method is 80 heads/female, and the average number of the F1 generations of Khattachar centipedes bred by the method is 51 heads/female. Therefore, the centipede fed by the method can greatly shorten the period of application of the centipede as a medicine, and effectively reduce the time period and other costs.

TABLE 4 statistics of Khattachar F1 generation number

Note: data in the table are mean ± sem.

The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.

Claims (10)

1. The breeding method of Khatherum japonicum is characterized by comprising the following steps:

s1, culturing the centipedes with the climatic chamber to the oviposition stage, picking out the female centipedes with the oviposition stage after one week of oviposition, controlling the temperature in the climatic chamber to be 18-34 ℃, the humidity to be 60-80% and the light cycle to be dark during the culturing period: illumination 12: 12;

s2, transferring the picked brooding Khattai centipedes to feeding boxes for independent feeding, placing the feeding boxes in a climatic box, and paving 1.5-2.5 cm-high padding in the feeding boxes, wherein the padding is prepared from coconut chaff and brick red soil according to the ratio of 7: (2-4), adding 15-25mL of traditional Chinese medicine liquid into the padding, and adjusting the humidity of the padding to be 20% -28% by using water, wherein the traditional Chinese medicine liquid is prepared from Dai Baijie and kidney tea;

s3, transferring the brood Kjellyfish centipedes from the feeding box to a climatic box to be fed separately from the larva after the brood Kjellyfish centipedes are hatched, transferring the brood Kjellyfish centipedes from the feeding box to the climatic box to be fed when the brood centipedes can move independently, and feeding the brood centipedes to an adult;

during the raising period of the steps S1 to S3, 7-9g of feed is fed to the centipedes every time in the non-oviposition period, the feed fed every time in the oviposition period is increased to 10-12g, the centipedes are not fed in the hatching period, the centipedes are observed and fed once every 3 days, and the feed for feeding comprises living animals and common meat.

2. The breeding method of centipedes hastelling as claimed in claim 1, wherein the temperature of the climatic chamber is 22-30 ℃.

3. The breeding method of centipedes hastelli according to claim 1, wherein the preparation method of the traditional Chinese medicine liquid comprises the following steps: mixing Daibaijie and herba Clerodendranthi Spicati at a weight ratio of 1:2, boiling in water, decocting to one fourth of the original volume, and filtering the residue to obtain the Chinese medicinal liquid.

4. The breeding method of centipedes harveyi according to claim 1, wherein the mass ratio of the living animals to the common meat in the feed is 1: 1.

5. The breeding method of centipedes hastelling according to claim 4, wherein the living animals are any one of crickets, locusts, grasshoppers, yellow mealworms and snails, the common meat is any one of fresh chicken, chicken livers and pig livers, and the living animals and the common meat are matched at will and different matching combinations are changed for each feeding.

6. The breeding method of centipedes hastella according to claim 1, wherein the padding is prepared from coconut coir and brick red soil according to the weight ratio of 7: 3 in a weight ratio.

7. The breeding method of centipedes hastella according to claim 1, wherein the laying height of the padding is 2.0 cm.

8. The breeding method of centipedes hastella according to claim 1, wherein 30-60 centipedes are bred in each climatic chamber.

9. The breeding method of centipedes hastelling as claimed in claim 1, wherein 20-40 rearing boxes are placed in each climate box.

10. The method for breeding centipedes hatsche according to claim 1, wherein during the breeding period of steps S1 to S3, the climate box is kept quiet without excessive disturbance except for daily breeding management.

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