CN113207605B - Control method for regulating flowering speed of agapanthus and agapanthus culture method - Google Patents
Control method for regulating flowering speed of agapanthus and agapanthus culture method Download PDFInfo
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- CN113207605B CN113207605B CN202110534833.6A CN202110534833A CN113207605B CN 113207605 B CN113207605 B CN 113207605B CN 202110534833 A CN202110534833 A CN 202110534833A CN 113207605 B CN113207605 B CN 113207605B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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Abstract
The invention discloses a control method for regulating and controlling the flowering speed of agapanthus, which comprises the following steps: in the bud stage of the agapanthus, the agapanthus is transferred to different light qualities for culture so as to control the agapanthus to bloom in advance or delay the blooming; wherein, when the transferred light quality is blue light, the agapanthus flowers in advance; when the transferred light quality is green light, the agapanthus praecox flowers are delayed. In addition, the invention also discloses a method for culturing agapanthus. After the agapanthus bracteatum bract is produced, the agapanthus bracteatum bract is transferred to different light qualities for cultivation for a period of time according to the requirements of advancing or delaying the time for starting to flower and the flowering speed, and then the cultivation under normal light is carried out. The invention aims at the agapanthus praecox with buds formed, and finely regulates and controls the flowering starting time and the flowering speed of the agapanthus praecox.
Description
Technical Field
The invention relates to the technical field of agapanthus blooming regulation, in particular to a method for regulating agapanthus blooming speed and application.
Background
Agapanthus praecox (Agapanthus praecox) is a plant of the genus Agapanthus (Agapanthus) of the family lycoriaceae (amarylidaceae), and is native to southern part of south africa. The agapanthus as an excellent garden ball perennial root flower is introduced into China for nearly twenty years, and through cultivation and observation, the agapanthus has strong adaptability and few plant diseases and insect pests, can be used for cultivation of pot flowers, flower beds and flower mirrors, can also be used as fresh cut flowers, and has a very wide application range. The agapanthus florescence is 6-9 months, and the flower is bluish purple and white and has large flower, spotted leaf, dwarf and other varieties. The plant cultivation method is popular in domestic garden application and flower market due to high ornamental value, strong adaptability and extensive cultivation management.
In the Netherlands, the annual supply of the cut flowers of the African agapanthus is basically realized, but a large-scale production system of the African agapanthus is not established in China, and the annual production and supply of the African agapanthus cannot be realized, so that the market popularization and application of the African agapanthus are limited. Because the open period of the African agapanthus is more concentrated, researchers do some exploration work in the adjustment and control research of the African agapanthus period. For example, the blooming time of the agapanthus can be advanced by 66 days or delayed by about 12 days by regulating the differentiation rate of the agapanthus buds through temperature change regulation, spraying exogenous growth regulators (such as gibberellin, indoleacetic acid and chlormequat chloride) and other measures. However, when the flower buds are differentiated and buds are formed, if the flowering time is finely controlled, no mature method, especially delayed flowering, exists at present.
The light is the environmental condition necessary for plant growth, different photoperiods, illumination intensity and light quality all have very important influence on the growth of plants, and simultaneously, the light also has the function of regulating and controlling the flowering of the plants. However, the influence of photoperiod on the flowering of agapanthus has not been reported. Although blue light or red light is reported to have the effect of promoting plant flowering, the red light or blue light has different regulation and control effects on different species, and the treatment timing, dosage and duration are also different. The influence of other lights on plant flowering is not yet clearly defined.
In the face of more and more application forms and high-requirement seedling quality, especially for the requirement of agapanthus flowering consistency, the single regulation and control of the agapanthus flowering period is not enough to meet the market requirement. Therefore, the technology for regulating and controlling the flowering time and the single flowering phase of the plants with buds, which are subjected to flower bud differentiation, is more important.
Disclosure of Invention
In view of the above disadvantages of the prior art, the present invention aims to provide a method for regulating the flowering speed of African agapanthus, and an application thereof, so as to control the flowering time and the flowering speed of African agapanthus, improve the flowering consistency, and realize the regulation of the flowering period of a single plant.
In order to achieve the purpose, the purpose of the invention is realized by the following technical scheme:
in a first aspect, the invention provides a control method for regulating and controlling the flowering speed of agapanthus, which comprises the following steps:
in the bud stage of the agapanthus, the agapanthus is transferred to different light qualities for culture so as to control the agapanthus to bloom in advance or delay the blooming;
wherein, when the light quality is changed into blue light, the agapanthus flowers in advance;
when the transferred light quality is green light, the agapanthus praecox flowers are delayed.
Preferably, the agapanthus bract period specifically refers to the early stage of agapanthus bract dehiscence.
Preferably, the agapanthus bracteatum bract period lasts for 1-14 days.
Preferably, the culture time is 2-7 days after transferring to different light qualities.
Preferably, when the transfer light quality is blue light, the blue light is single light quality blue light, and the specific wavelength in the blue light region is 440 nm-470 nm.
More preferably, the intensity of the blue light is 1300-.
Preferably, when the transfer-in light quality is green light, the green light is green light with single light quality, and the specific wavelength of the green light area is 520 nm-555 nm.
More preferably, the intensity of the green light is 1300-.
In a second aspect, the invention provides a method for culturing agapanthus, which comprises the following steps:
step S1: culturing the agapanthus plant in a normal light environment until the agapanthus plant is provided with a bud, thereby obtaining the agapanthus plant entering the bud stage of the agapanthus;
step S2: culturing the agapanthus plant entering the bud stage of the agapanthus obtained in the step S1 by the control method to control the flowering speed of the agapanthus plant,
preferably, in step S1, the normal light culture environment is specifically: the incubation temperature was 20 ℃ and 16h light/8 h dark.
According to the technical scheme, the agapanthus plant with the buds obtained in a normal light culture environment is used as a material, and the subsequent light regulation and control treatment is carried out at different time of the formation of the agapanthus buds according to the time length of advancing or delaying the flowering. If long-time delay or early flowering is needed, carrying out subsequent light control treatment 7 days after the buds are formed; if a short time of delay or early flowering is required, the subsequent light regulation treatment is carried out for 7-14 days after the formation of the buds.
Preferably, the agapanthus praecox plant with the buds is transferred into an incubator with different light qualities for cultivation under the conditions of constant temperature of 25 ℃, 16 hours of light in photoperiod and 8 hours of darkness. When the light quality is blue light (the wavelength is 440-470 nm) and the light intensity is 1500lx, the control effect is to bloom in advance; when the light quality is green light (the wavelength is 520 nm-555 nm) and the light intensity is 1500lx, the regulating and controlling effect is to delay the flowering.
Preferably, after the continuous culture is carried out for 2-7 days under a single specific light quality, the florescence regulation effect can be obviously seen, and then the culture is continued under the normal illumination condition.
Compared with the prior art, the invention has the following beneficial effects:
(1) the invention does not limit the seedling culture environment of the agapanthus, and only cultures seedlings with different light qualities in specific requirements in specific periods, the culture time is short, and the culture environment is easy to obtain.
(2) The invention is suitable for emergently controlling the flowering time under specific requirements, or adjusting the flowering period of individual plants under the condition of inconsistent seedlings so as to realize the group flowering consistency.
Drawings
FIG. 1 is a photograph of African agapanthus after 2 days of treatment in examples 1 and 2;
FIG. 2 is a photograph of African agapanthus after 7 days of treatment in examples 1 and 2;
FIG. 3 is a photograph of agapanthus after normal culture for 7 days after treatment in examples 1 and 2;
FIG. 4 is a photograph of African agapanthus after 7 days of treatment in comparative example 1.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that it would be obvious to those skilled in the art that various changes and modifications can be made without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
The experiment is carried out in a culture room and a light incubator of the institute of agriculture and biology of Shanghai transportation university in 3 months in 2020. The agapanthus perennial plant is purchased from a company in 2019 in 11 months, is placed in a culture room for culture under the conditions of 20 ℃ and 16h light/8 h darkness, and is cultured until the beginning of 5 months in 2020, the agapanthus scapus is extracted, and the buds are formed.
In 5 months of 2020, the agapanthus plant with the buds is transferred to a single blue light (the wavelength is 440-470 nm, the light intensity is 1500lx) incubator for cultivation, and meanwhile, the agapanthus plant with the buds in the same development process is transferred to a white light incubator, and the cultivation environment is constant at 25 ℃, the light cycle is 16 hours, and the light is 8 hours dark. After 2 days and 7 days of culture, the cells were taken out and recorded by photographing. After 7 days of treatment, the cells were removed from the incubator, returned to the incubator for culture, and recorded by photographing after 7 days.
The experimental results are as follows:
as shown in fig. 1, after 2 days of culture, the blue-light treated agapanthus buds cracked, and the pedicel extended and protruded significantly, while the white-light treated agapanthus buds cracked, but the pedicel was shorter and protruded less significantly than that of the blue-light treated agapanthus buds. As shown in fig. 2, after 7 days of culture, the buds of the white-light treated agapanthus fully developed and the flowers developed completely, while the buds of the white-light treated agapanthus were still small and did not start to stain. As shown in FIG. 3, after being placed back into the culture chamber for culture for 7 days, the flowering processes of the two groups of treated agapanthus still have great difference, which shows that the blue light treatment obviously improves the flowering speed of the agapanthus, but has no obvious influence on the growth and development of other aspects. Statistics shows that the blooming of the agapanthus can be advanced by about 10 days after the blue light treatment.
Example 2
The treatment method is the same as that of example 1, except that the agapanthus plant with buds is transferred to a single green light (wavelength is 520-555 nm, light intensity is 1500lx) incubator for culture, and meanwhile, the agapanthus plant with buds in the same development process is transferred to a white light incubator, wherein the culture environment is constant temperature of 25 ℃, 16h light period and 8h dark period. After 2 days and 7 days of culture, the cells were taken out and recorded by photographing. After 7 days of treatment, the cells were removed from the incubator, returned to the incubator for culture, and recorded by photographing after 7 days.
The experimental results are as follows:
as shown in fig. 1, after 2 days of culture, green-treated agapanthus buds had not cracked, while white-treated agapanthus buds had cracked. As shown in fig. 2, after 7 days of culture, the green-treated agapanthus bud was still in a semi-dehiscent state, and the flower was growing, yet uncolored, while the white-treated agapanthus bud was completely dehiscent, and the flower was growing, yet uncolored. As shown in FIG. 3, after being placed back into the culture chamber for 7 days, the flowering process of the two groups of treated agapanthus still has difference, but the difference is reduced compared with the treatment, which indicates that the green light treatment obviously reduces the flowering speed of the agapanthus, but the inhibition effect is weakened after the green light treatment is removed. Statistics shows that the green light treatment can delay the blooming of the agapanthus for about 5 days.
Comparative example 1 light quality Using Red light
The treatment method is the same as that of example 1, except that the agapanthus plant with buds is transferred to a single red light (with the wavelength of 640-660 nm) incubator for culture, and the culture environment is constant temperature of 25 ℃, 16 hours of light and 8 hours of dark light in a light cycle. After 7 days of culture, the cells were taken out and recorded by photography. After 7 days of treatment, the cells were removed from the incubator, returned to the incubator for culture, and recorded by photographing after 7 days.
The experimental results are as follows:
as shown in fig. 4, after 7 days of culture, in the figure, the red-treated agapanthus bracteatum bud is not completely unfolded yet, and in the figure, the blue-treated agapanthus bracteatum bracten bud is completely unfolded, which shows that the blue-light treatment significantly improves the flowering speed of the agapanthus.
It should be noted that, in the existing research process of agapanthus flowering, how different light qualities regulate and control agapanthus flowers is not found, the inventor of the present application finds that blue light and green light can significantly affect agapanthus flowering speed through a large number of experimental studies, and verifies that the blue light and green light qualities can actually affect and change agapanthus flowering time through experiments of examples 1 and 2, and the finding controls flowering speed of flowers in the processes of agapanthus pot flower production and fresh-cut flower preservation period so as to improve commercial value of the flowers. Also has great value in garden application and plant cultivation.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (4)
1. A control method for regulating and controlling the blooming speed of African agapanthus, which is characterized by comprising the following steps:
in the bud stage of the agapanthus, the agapanthus is transferred to different light qualities for culture so as to control the agapanthus to bloom in advance or delay the blooming;
wherein, when the light quality is changed into blue light, the agapanthus flowers in advance;
when the transferred light quality is green light, the agapanthus praecox flowers are delayed;
the agapanthus bud period specifically refers to a non-dehiscence period of the agapanthus bud;
transferring the culture medium to different light qualities for 2-7 days;
when the transferred light quality is blue light, the blue light is single light quality blue light, and the specific wavelength of a blue light area is 440 nm-470 nm;
the intensity of the blue light is 1300-;
when the transferred light quality is green light, the green light is green light with single light quality, and the specific wavelength of a green light region is 520-555 nm;
the green light intensity is 1300-.
2. The control method according to claim 1, wherein the agapanthus fragrans bud period lasts for 1-14 days.
3. The agapanthus cultivation method is characterized by comprising the following steps:
step S1: culturing the agapanthus plant in a normal light environment until the agapanthus plant is provided with buds, thereby obtaining the agapanthus plant entering the bud stage of the agapanthus;
step S2: the agapanthus plant entering the bud stage of agapanthus obtained in step S1 is cultured by the control method according to any one of claims 1 to 2 to control the flowering rate of the agapanthus plant.
4. The agapanthus cultivation method according to claim 3, wherein in step S1, the normal light cultivation environment is specifically: the incubation temperature was 20 ℃ and 16h light/8 h dark.
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| CN103483437A (en) * | 2013-09-22 | 2014-01-01 | 东北林业大学 | Protein of key gene ApCO for photoperiod and flowering pathway of agapanthus praecox ssp.orientalis as well as coding gene and probe of gene |
| USPP25785P2 (en) * | 2014-01-13 | 2015-08-04 | The Pink Geranium Nursery | Agapanthus plant named ‘Croft's Pearl’ |
| CN110224053A (en) * | 2019-05-07 | 2019-09-10 | 广东绿爱生物科技股份有限公司 | A kind of LED light source and its preparation method and application and it is a kind of regulate and control the plant florescence method |
| CN110447497A (en) * | 2019-08-16 | 2019-11-15 | 厦门市园林植物园 | A method of promote noble woman begonia to bloom |
| CN111418470A (en) * | 2020-05-26 | 2020-07-17 | 山东省烟台市农业科学研究院 | Cultivation method for regulating and controlling hydrangea florescence |
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