CN113191466A - Biological sample management method for scientific research - Google Patents
Biological sample management method for scientific research Download PDFInfo
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- CN113191466A CN113191466A CN202011630178.6A CN202011630178A CN113191466A CN 113191466 A CN113191466 A CN 113191466A CN 202011630178 A CN202011630178 A CN 202011630178A CN 113191466 A CN113191466 A CN 113191466A
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- 238000007726 management method Methods 0.000 title claims abstract description 111
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
- G06K17/00—Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations
- G06K17/0022—Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations arrangements or provisious for transferring data to distant stations, e.g. from a sensing device
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
- G06K17/00—Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations
- G06K17/0022—Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations arrangements or provisious for transferring data to distant stations, e.g. from a sensing device
- G06K17/0029—Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations arrangements or provisious for transferring data to distant stations, e.g. from a sensing device the arrangement being specially adapted for wireless interrogation of grouped or bundled articles tagged with wireless record carriers
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- G—PHYSICS
- G07—CHECKING-DEVICES
- G07C—TIME OR ATTENDANCE REGISTERS; REGISTERING OR INDICATING THE WORKING OF MACHINES; GENERATING RANDOM NUMBERS; VOTING OR LOTTERY APPARATUS; ARRANGEMENTS, SYSTEMS OR APPARATUS FOR CHECKING NOT PROVIDED FOR ELSEWHERE
- G07C9/00—Individual registration on entry or exit
- G07C9/00174—Electronically operated locks; Circuits therefor; Nonmechanical keys therefor, e.g. passive or active electrical keys or other data carriers without mechanical keys
- G07C9/00571—Electronically operated locks; Circuits therefor; Nonmechanical keys therefor, e.g. passive or active electrical keys or other data carriers without mechanical keys operated by interacting with a central unit
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention aims to provide a biological sample management method for scientific research, which can effectively manage biological samples and comprises the following steps: correspondingly storing the sample distinguishing information, the corresponding sample name, the sample position information and the storage requirement information as sample related information; adopting an identification part to identify, and then sending identification information to a management server; judging whether the scientific research personnel have the opening authority at least based on the identification information, and sending an opening permission signal to the sample cabinet once the scientific research personnel have the opening authority; the door of the cabinet is unlocked according to the door opening permission, so that scientific research personnel can open the door of the cabinet to carry out extraction or storage operation; and the judgment part is adopted to continuously perform the reading action, and once the cabinet door is closed, at least the read sample judgment information and the cabinet number of the sample cabinet are sent to the management server, so that the access condition of the biological sample is judged, and at least the sample position information is updated.
Description
Technical Field
The invention relates to a biological sample management method for scientific research.
Background
The biological sample refers to organs, tissues, cells, biological macromolecules and the like derived from human bodies, other animals or plants, and is used as a sample in scientific research work. Among them, important resource facilities in the scientific research of biological sample libraries composed of one or more types of biological samples, for example, in molecular genetics, some research projects require a large number of genetic resource samples, and thus need to utilize the human genome library for completion; pathological studies typically require a large number of pathological tissue samples and therefore rely on a library of pathological samples.
In order to ensure the rich diversity of biological samples, a large amount of biological samples need to be stored and managed in the process of establishing and subsequent maintenance of the biological sample library. Meanwhile, the storage conditions of these biological samples are harsh, and generally require environments such as a low-temperature freezer (-30 below) or an ultra-low-temperature freezer (-80 ℃) and even liquid nitrogen. Due to the large number and harsh conditions, the management of biological samples is often confused, for example, when it is necessary to take a biological sample, it is not clear where to store it, and when it is returned, it is not clear where to return it. Further, such circumstances make scientific research personnel take or need spend the long time to open the cabinet door and look over when returning, and in case the time of opening the door overlength, the temperature in low temperature or the ultra-low temperature freezer just rises by the external influence easily, even close the cabinet door also need the certain time cooling to resume, other biological samples in the cabinet still will receive the influence or even probably become invalid.
In order to solve the above problem, some sample libraries may use a method of dividing a specific storage area, which can solve the problem of disordered storage of samples to some extent. However, such a method requires that the scientific research personnel must go to a specific place when taking or storing the materials, which is inconvenient; meanwhile, the situation that one part of storage cabinets is fully stacked and the other part of storage cabinets is vacant is easy to occur.
Disclosure of Invention
In order to solve the problems, the invention provides a biological sample management method for scientific research, which can effectively manage biological samples, performs information management on the biological samples based on sample tags, and further provides a biological sample management method for scientific research, which is characterized in that a system comprising a sample cabinet, a scientific research personnel terminal and a management server is adopted to realize the management on the biological samples with the sample tags, and the method comprises the following steps: the sample discrimination information, the corresponding sample name, the sample position information and the storage requirement information are used as sample related information to be correspondingly stored in the management server; identifying the identification information of scientific research personnel by adopting an identification part of the sample cabinet, and then sending the identification information to a management server; judging whether the scientific research personnel have the opening authority or not at least based on the identification information by adopting a management server, and sending an opening permission signal to the sample cabinet once the scientific research personnel have the opening authority; the control module of the sample cabinet releases the locking of the cabinet door according to the door opening permission so as to enable scientific researchers to open the cabinet door for extraction or storage operation; the method comprises the steps that a judging part is adopted to continuously read and an induction part is adopted to induce whether a cabinet door is closed or not, and once the door is induced to be closed by scientific research personnel, at least read sample judging information and a cabinet number of a sample cabinet are sent to a management server; the management server judges the access condition of the biological sample according to the sent sample judgment information and the cabinet number, and at least updates the sample position information in the sample related information.
Action and Effect of the invention
According to the biological sample management method for scientific research provided by the invention, the sample labels are arranged on the biological samples, and the sample labels are distinguished in the distinguishing part of the sample cabinet, so that the biological labels on the biological samples are distinguished after the cabinet door is opened by scientific research personnel, and the corresponding biological sample distinguishing information is obtained, so that the management server can judge the access condition of the biological samples according to the biological sample distinguishing information and update the sample position information, and the positions of the biological samples can be effectively obtained and timely updated and stored, thereby facilitating the access operation in the subsequent use process. In addition, because the opening and closing of the induction part induction cabinet door is adopted, the judgment part is allowed to judge when the cabinet door is opened, so that the sample labels can be continuously read under the states that the cabinet door is closed and the sample cabinet is not used, the working time of the judgment part is shortened, the service life of the judgment part is prolonged, and the problem that the reading work of the cabinet door on the judgment part is influenced due to the fact that the cabinet door is read under the condition that the cabinet door is closed is also avoided.
Drawings
FIG. 1 is a diagram showing a system for implementing a biological specimen management method for scientific research in an embodiment of the present invention.
FIG. 2 is a schematic diagram of a sample cabinet according to an embodiment of the present invention;
FIG. 3 is a schematic view of an installation location of an RFID reader of an embodiment of the present invention;
FIG. 4 is a range diagram of an RFID reader of an embodiment of the present invention;
FIG. 5 is a functional block diagram of a sample cabinet in an embodiment of the invention;
FIG. 6 is a schematic diagram of the storage contents of the cabinet number layer number storage unit in the embodiment of the present invention;
FIG. 7 is a flowchart of a scientific biological specimen management method according to an embodiment of the present invention;
FIG. 8 is a schematic diagram of sample correlation information storage according to an embodiment of the present invention;
FIG. 9 is a schematic diagram of the storage contents of the request record storage unit in the embodiment of the present invention; and
fig. 10 is a schematic diagram of extraction record storage in the embodiment of the present invention.
Detailed Description
The method for managing a biological sample for scientific research according to the present invention will be described in detail below with reference to the accompanying drawings.
The invention provides a biological sample management method for scientific research, which is characterized in that a system comprising a sample cabinet, a scientific research personnel terminal and a management server is adopted to realize the management of a biological sample with a sample label, and the method comprises the following steps: the sample discrimination information, the corresponding sample name, the sample position information and the storage requirement information are used as sample related information to be correspondingly stored in the management server; identifying the identification information of scientific research personnel by adopting an identification part of the sample cabinet, and then sending the identification information to a management server; judging whether the scientific research personnel have the opening authority or not at least based on the identification information by adopting a management server, and sending an opening permission signal to the sample cabinet once the scientific research personnel have the opening authority; the control module of the sample cabinet releases the locking of the cabinet door according to the door opening permission so as to enable scientific researchers to open the cabinet door for extraction or storage operation; the method comprises the steps that a judging part is adopted to continuously read and an induction part is adopted to induce whether a cabinet door is closed or not, and once the door is induced to be closed by scientific research personnel, at least read sample judging information and a cabinet number of a sample cabinet are sent to a management server; the management server judges the access condition of the biological sample according to the sent sample judgment information and the cabinet number, and at least updates the sample position information in the sample related information.
The biological sample management method for scientific research according to the above-described embodiment may further include a technical feature in which the management server updates the sample position information in the sample-related information and stores the sample discrimination information corresponding to the biological sample determined to be extracted and the identification information received from the sample holder as one extraction record.
Further, the biological sample management method for scientific research may further have a technical feature that a scientific researcher inputs a name of a biological sample to be extracted as a name of a required sample through a scientific researcher terminal and sends the name of the required sample to the management server through the scientific researcher terminal, the management server retrieves consistent sample discrimination information from sample related information according to the received name of the required sample, thereby further acquiring sample position information, and sends the retrieved sample position information and the received name of the required sample as extraction information to the scientific researcher terminal.
Further, the above biological sample management method for scientific research may further have a technical feature in that the management server transmits the extraction information to the scientific research personnel terminal and stores the name of the required sample, the extraction information, and the identification information of the scientific research personnel received from the scientific research personnel terminal as a request record.
Further, the biological sample management method for scientific research can also have the technical characteristics that the management server judges whether the scientific research personnel has the opening authority or not according to the extraction record, the request record and the received identification information, and once the scientific research personnel is judged to have the opening authority, the management server sends an opening permission signal to the sample cabinet.
Further, the biological sample management method for scientific research can also have the technical characteristics that the management server judges whether scientific research personnel have the opening authority or not and carries out the operation based on the extraction record and the request record, and the specific process is as follows: respectively retrieving the request records according to the received identification information and the cabinet number, judging whether the matched request records exist, and further judging whether the cabinet number exists in the sample position information of the matched request records when the matched request records exist; and searching the extracted records according to the received identification information, judging whether matched extracted records exist or not, further acquiring corresponding storage requirement information from sample related information according to sample judgment information in the extracted records when the matched extracted records exist, judging whether the sent cabinet number exists in the acquired storage requirement information or not, and judging that the scientific research personnel have the opening authority if the final result of any judgment exists in the two judgments.
Further, the method for managing biological samples for scientific research may further include: the management server further judges whether the biological sample extracted by the scientific research personnel is correct or not according to the judgment result of the access condition and the extraction record, judges whether the position where the biological sample is stored is correct or not according to the judgment result of the access condition and the corresponding storage requirement information, and sends a warning signal to the corresponding sample cabinet when the scientific research personnel is judged to be wrong in extraction or storage.
Further, the above method for managing a biological sample for scientific research may further have a technical feature that the process of determining whether the extracted biological sample is correct is as follows: and acquiring a corresponding biological sample name from the extraction record as an extraction name according to the identification information, acquiring a corresponding biological sample name from the sample related information as an actual extraction name according to the received biological sample discrimination information, further judging whether the extraction name is consistent with the actual extraction name, and judging that the extraction of scientific researchers is wrong when the extraction name is not consistent with the actual extraction name.
Further, the above method for managing a biological sample for scientific research may further have a technical feature in which the process of determining whether the stored biological sample is correct is as follows: and searching and acquiring corresponding storage requirement information from the sample related information according to the sample identification information, further judging whether the position information at least comprising the cabinet number for storing the biological sample exists in the acquired storage requirement information, and judging that the scientific research personnel has a storage error when judging that the position information does not exist.
< example >
FIG. 1 is a diagram showing a system for implementing a biological specimen management method for scientific research in an embodiment of the present invention.
As shown in fig. 1, the biological sample management method for scientific research of the present embodiment is implemented by using a system 100, and the system 100 includes a plurality of sample cabinets 1, a management server 2, at least one scientific research personnel terminal 3, and communication networks 4a and 4 b.
The management server 2 is respectively in communication connection with each sample cabinet 1 through a communication network 4a and is respectively in communication connection with each scientific research personnel terminal through a communication network 4 b. In this embodiment, the communication networks 4a and 4b are both local area networks.
The scientific research personnel terminals 3 are personal computers, are respectively held by different scientific research personnel and carry out related operations, and identification information corresponding to the respective scientific research personnel is stored in each scientific research personnel terminal 3. The scientific researchers involved in the present invention may be sample library management personnel who manage the biological sample 200, or may be scientific research experimenters who have access rights to the biological sample 200. In addition, the biological samples 200 are physiological or pathological samples of human body, other animals or plants obtained by sampling, including but not limited to organs, tissues, cells and biological macromolecules, and the forms of the biological samples 200 and the containers thereof may be the same or different according to the preservation requirement; either as containers containing a single sample (e.g., tubes, bags or bottles) or as containers containing multiple aliquots of the same sample (e.g., cartridges containing multiple tubes or tubes). A corresponding sample tag 201 is fixed to each container of the biological sample 200, and the sample tag 201 is a low-temperature RFID tag containing sample discrimination information and can normally operate in low-temperature and ultra-low-temperature environments.
Fig. 2 is a schematic structural diagram of a sample cabinet in an embodiment of the invention. In fig. 2, the storage rack and the storage cassette for storing the biological samples 200 are omitted to show the structure more clearly.
As shown in fig. 2, the sample chamber 1 includes a chamber body 10, a placement layer 11, a chamber door 12, a locking portion 13, a sensing portion 14, a determination portion 15, an identification portion 16, a warning portion 17, and a main control chip 18. In this embodiment, sample cabinet 1 still is equipped with cooling mechanism such as compressor, is used for carrying out the temperature setting mechanism that the temperature set for, is used for detecting the temperature detection mechanism who places layer 11 internal temperature and controls the control mechanism who realizes the control by temperature change according to temperature detection result and the work of setting for the temperature to cooling mechanism, thereby the specific constitution and the theory of operation of these mechanisms are the same with among the ultra-low temperature refrigerator among the prior art, and it is no longer repeated here.
Three placing layers 11 for placing the biological samples 200 are arranged in the cabinet 10, and the adjacent placing layers 11 are separated by a layer plate.
In this embodiment, the interlayer plate is a heat-insulating plate having a certain thickness. The interlayer sheet does not have any restriction structure for the sample, and thus the biological sample 200 can be freely placed on the placement layer 11.
The number of the cabinet door 12, the locking portion 13, the sensing portion 14, and the determination portion 15 is three, and each corresponds to each placement layer 11.
Wherein, the cabinet door 12 is hinged on the cabinet body 10 at the side position of the placing layer 11. Cabinet door 12 is the heat preservation cabinet door, by the metal level that is located the outside and be located the heat preservation of inboard and constitute, and the layer 11 is placed to the heat preservation orientation inside to realize the heat preservation function.
The locking portion 13 is an electronic lock, is disposed on the cabinet door 12 or on the cabinet body 10 at a position corresponding to the edge of the cabinet door 12, and can lock and unlock the cabinet door 12 according to the control electrical signal of the main control chip.
The sensing part 14 is a proximity switch, is disposed on each cabinet door 12 or on the cabinet body 10 at a position corresponding to the edge of each cabinet door 12, and can sense whether the corresponding cabinet door 12 is in a closed state and generate a corresponding electrical signal.
The discrimination unit 15 discriminates the specimen label 201 on the biological specimen 200 when the biological specimen 200 is taken out or placed.
Fig. 3 is a schematic view of an installation position of an RFID reader according to an embodiment of the present invention, and fig. 3 shows a partial sectional structure of a sample cabinet.
As shown in fig. 2 and 3, in the present embodiment, each determination unit 15 is constituted by two RFID readers 151. The upper interlayer board of each placement layer 11 extends outward to form a layer eaves portion 111, and the two RFID readers 151 are embedded in the layer eaves portions 111 of the upper interlayer boards of the placement layers 11 corresponding to the determination unit 15, and are located at both ends (corresponding to the left and right sides) in the width direction of the interlayer boards. Meanwhile, each RFID reader 151 is located at the outer edge position of the interlayer plate, and the position corresponds to the outer surface position of the cabinet door 12, that is, a space with a heat preservation effect is enclosed between the heat preservation layer of the cabinet door 12 and each interlayer plate of the placement layer 11, and the RFID reader is located outside the heat preservation space, so that the RFID reader is not affected by low temperature in the space and can work normally.
FIG. 4 is a range diagram of an RFID reader of an embodiment of the present invention. Wherein, fig. 4(B) is a schematic range diagram in the width direction, fig. 4(a) is a schematic range diagram in the depth direction, and the broken lines in the diagram each indicate the read range of the RFID reader; in addition, in fig. 4(B), the cabinet door 12 is omitted.
As shown in fig. 4(a), in the present embodiment, the read range D of the RFID reader 151 in width is greater than one-half of the width L of the interlayer board and smaller than the width L of the interlayer board. Since the RFID readers 151 are respectively located at both ends of the interlayer sheet, when the biological sample 200 is stored from a position near one end of the interlayer sheet (for example, placed in the placement layer 11 from a position near the left or right), only one RFID reader 151 near the biological sample 200 can read the sample label 201 thereon, and when the biological sample 200 is picked up or placed from a position near the middle of the interlayer sheet, both RFID readers 151 can read the sample label 201 thereon. For example, in fig. 4(a), a biological sample 200a is put in from the right area, and its biological tag 201a is read only by the RFID reader 151 on the right side during the putting in; the biological sample 200b is put in from the middle area, so that the biological tag 201a thereof can be read by two RFID readers 151 at the same time during the putting in process; the biological specimen 200a is put in from the left area, and the biological tag 201a thereof is read only by the RFID reader 151 on the left side during the putting in. Thus, the specimen rack control unit can determine the approximate location (left area, right area, or middle area) where the biological specimen 200 is stored, based on the fact that the two RFID readers 151 read the biological tag 201.
In addition, as shown in fig. 4(B), the reading direction of the RFID reader 151 in the present embodiment is not directed inward (i.e., is not directed toward the inside of the setting layer 11) when viewed from the depth direction, so that the RFID reader 151 cannot read the sample label 201 on the biological sample 200 in the setting layer 11 regardless of the opened or closed state of the cabinet door 12.
Specifically, the RFID reader 151 of the present embodiment is a directional RFID reader, and the directional RFID reader is installed slightly obliquely outward, that is, the reading direction of the directional RFID reader faces outward, so that the sample tag 201 is read only in the picking and placing process. For example, in fig. 4(B), once the cabinet door 12 is opened, the RFID reader 151 can read the bio-tag 201d on the biological sample 200d being put in, but cannot read the bio-tag 201e on the biological sample 200e already positioned in the setting layer 11. That is, since the reading directions of the RFID readers 151 are all facing outward, the sample tags 201 on the biological samples 200 in the placement layer 11 are not read, and the biological samples 200 can only pass through the reading area of the RFID readers 151 in the taking and placing processes, so that the sample tags 201 thereon are read.
In addition, in this embodiment, the interlayer plate further has a metal interlayer 112, and the metal interlayer 112 forms a shielding effect, so the RFID reader 151 does not read the sample tag 201 on the biological sample 200 in the adjacent placement layer 11.
The recognition unit 16 is provided on the cabinet 10 and recognizes recognition information of a researcher.
In this embodiment, the identification information is door opening information, a password, two-dimensional code information corresponding to the identity of a scientific researcher, fingerprint information of the scientific researcher, or face information in a door opening card held by the scientific researcher, and the identification portion is a door card reading device, a password input device, a two-dimensional code scanning device, a fingerprint identification device, or a face identification device corresponding to the identification information. The scientific researchers can operate the identification part 16 in a corresponding mode (namely, the operations of swiping a card, inputting a password, scanning a two-dimensional code, verifying a fingerprint, shooting a human face and the like) so that the identification part 16 can identify the identification information of the scientific researchers.
The identification information corresponds to the identities of the scientific research personnel respectively, namely, each scientific research personnel has own unique identification information, and the identities of the scientific research personnel can be directly and correspondingly determined through the identification information.
The warning portion 17 is a warning light disposed on the front surface of the cabinet 10, and is configured to generate a light flash when receiving the warning message.
Fig. 5 is a functional block diagram of a sample cabinet in an embodiment of the invention.
As shown in fig. 5, the main control chip 18 is electrically connected to the locking portion 13, the sensing portion 14, the determination portion 15, the identification portion 16, and the warning portion 17, respectively, and controls the operations of these components. The main control chip 18 includes a sample cabinet communication section 181 and a cabinet number layer number storage section 182.
The sample chamber communication unit 181 is a communication module connected to the main control chip 18, and is used to perform data communication between the sample chamber 1 and the management server 2.
In this embodiment, the cabinet number layer number storage unit 182 records the respective cabinet number, and the scientific research personnel can find the corresponding sample cabinet 1 through the cabinet number. Meanwhile, in each sample cabinet 1, the respective placement layers 11 also have respective unique layer numbers. Since the determination unit 15 corresponds to each placement layer 11, actually, the determination unit 15 also corresponds to each layer number, so that when the determination unit 15 reads the sample label 201, the main control chip 18 can determine which determination unit 15 of which placement layer 11 has read the sample label according to the correspondence. For convenience of description, hereinafter, the three set layers 11 are referred to as a set layer 11a, a set layer 11b, and a set layer 11c, and the determination units 15 corresponding to the set layer 11a, the set layer 11b, and the set layer 11c are referred to as a determination unit 15a, a determination unit 15b, and a determination unit 15c, respectively.
Fig. 6 is a schematic diagram of the storage contents of the cabinet number layer number storage unit in the embodiment of the present invention.
As shown in fig. 6, the sample tank 1 having the tank number "GH 1" shown in fig. 6 is exemplified by having three storage layers 11, the layer numbers of which are "CHa", "CHb" and "CHc", respectively, and the numbers of the three discrimination sections are "PBa", "PBb" and "PBc", respectively.
In addition, similarly, since the locking part 13 and the sensing part 14 also correspond to the placing layer 11, the main control chip 18 can also perform corresponding control of locking or unlocking according to the corresponding relationship, or obtain the sensing result of the opening/closing state of the corresponding cabinet door 12.
The management server 2 is used for storing and managing the biological sample 200 and the related information thereof.
The scientific research personnel terminal 3 is in communication connection with the management server 2 through a communication network 4, and is used for enabling scientific research personnel to perform corresponding human-computer interaction including information input, information checking and the like.
FIG. 7 is a flowchart of a scientific biological sample management method according to an embodiment of the present invention.
As shown in fig. 7, the biological sample management method for scientific research according to the present embodiment is implemented based on the system 100 of the sample cabinet 1, the server 2, the researcher terminal 3, and the communication network 4, and is used for managing information of a biological sample 200 based on a sample tag 201 including an RFID tag, and specifically includes the following steps.
Step S1, the sample discrimination information, the corresponding sample name, the sample position information, and the storage request information are stored as sample-related information in correspondence. Specifically, these pieces of information are stored in the management server 2.
Fig. 8 is a schematic diagram of sample related information storage in the embodiment of the present invention.
As shown in fig. 8, in the present embodiment, the specimen discrimination information 211 is unique number information of the biological specimen 200, which is also recorded in the chip of the specimen tag 201, and thus the number information can be obtained by an RFID reader once the specimen tag 201 is read.
The specimen name 212 is the name of the specimen contained in the specimen bottle.
The sample position information 213 includes the cabinet number of the sample cabinet 1, the layer number of the placement layer 11, and the position in the placement layer 11. Each sample position information 213 takes the form of a bin number and a layer number. Taking 1 having a bin number GH1 and including three placement levels 11 as an example, as shown in fig. 8, the first few bits of each sample position information 213 are the corresponding bin number, the last few bits are the level number, and the last few bits are the positions (left, middle, or right) in the placement level 11, thereby avoiding the situation where the same sample position information 213 occurs in the same number of placement levels 11 of different sample bins 1.
The storage request information 214 includes the bin number and the layer number of the sample bin 1 in which the biological sample 200 can be stored. Due to different storage requirements of different biological samples 200, the samples may be stored in the sample cabinets 1 with different set temperatures due to temperature requirements, and the samples with the same storage temperature may be stored separately due to sample storage safety requirements (for example, a physiological sample and a pathological sample should be stored separately, and a sample containing an infectious pathogen should be completely isolated from other samples). In addition, since the storage layers 12 are separated by the layer plates, they can be regarded as an independent storage environment, and the storage requirement information includes the number of the sample cabinet 1 in which a certain biological sample 200 can be stored and the layer number (i.e., the number of the sample cabinet 1 and the layer number that satisfy the storage requirements in various aspects such as the temperature requirement and the isolation requirement of the biological sample 200). The storage requirement information 214 of each biological sample 200 may have a plurality of bin number layer numbers (i.e., the biological sample 200 may be placed in any one of the corresponding plurality of placement layers 11), or may have only one (i.e., the biological sample 200 may be placed in only one of the corresponding placement layers 11), according to the storage requirement.
In this embodiment, the sample discrimination information 211 may have the same sample name (for example, if the biological samples 200 are formed by sub-packaging the same type of sample from the same source, the biological samples 200 all have the same sample name). Meanwhile, the plurality of sample discrimination information 211 may correspond to the same sample position information 212 (for example, a plurality of biological samples 200 are carried on a placement layer of the same sample cabinet). The sample name 212 may also include some information in the form of numbers, such as a sampling number, according to the naming requirement, but the number information in the sample name 212 and the number of the sample identification information may be independent of each other and have no correspondence. In addition, the storage request information 214 corresponding to each sample discrimination information 211 may include a plurality of the cabinet numbers and the layer numbers, for example, if a certain biological sample 200 may be stored in a plurality of the storage layers 12 of different sample cabinets 1, the storage request information 214 includes all the cabinet numbers and the layer numbers thereof that satisfy the storage request.
In this embodiment, the information may be entered and stored by a manager using the management terminal to log in the management server 2 while storing a newly collected biological sample, or the information may be stored by importing the existing information into the management server 2. In addition to the above-described information association storage, other necessary related information such as the sampling source and the sampling time of the biological sample 200 may be associated with the sample discrimination information.
After the initial storage of the information is completed, the scientific researchers can extract or store the biological sample 200.
When a researcher applies for a sample, the researcher terminal 3 is required to log in the management server 2 by using the identification information, and the name of the required sample is input through the man-machine interaction picture of the researcher terminal 3. Subsequently, the scientific research personnel terminal 3 sends the input required sample name to the management server 2, and the management server 2 retrieves consistent sample discrimination information from the sample related information according to the received required sample name, so as to further obtain the sample position information, and sends the retrieved sample position information and the received required sample name as extraction information to the scientific research personnel terminal 3. In the extracted information, the sample position information corresponding to one demand sample name may be plural.
Fig. 9 is a schematic diagram of the storage content of the request record storage unit in the embodiment of the present invention.
As shown in fig. 9, the management server 2 also stores the name of the required sample, the extracted information, and the identification information received from the terminal 3 of the researcher as one request record 231 while transmitting the extracted information. In this embodiment, when the scientific research staff selects a plurality of demand samples at the same time, the request record storage unit 23 also stores the names of the demand samples and the corresponding extraction information and identification information as different request records 231.
Step S2, when a scientific research staff selects a sample cabinet 1 to take and put biological samples, the identification information of the scientific research staff is identified by the identification part, then the identification information is sent to the management server through the sample cabinet communication module, and then the step S3 is carried out.
In step S3, the management server 2 determines whether the scientific research personnel has the opening authority or not according to the extraction record, the request record and the received identification information, and sends an opening permission signal to the sample cabinet 1 once the scientific research personnel has the opening authority, and then the process goes to step S4.
In this embodiment, the management server 2 further stores the extraction record.
Fig. 10 is a schematic diagram of extraction record storage in the embodiment of the present invention.
As shown in fig. 10, when a scientific researcher takes out a certain biological sample 200, the management server 2 stores the corresponding sample discrimination information and the identification information received from the sample cabinet 1 as one extraction record 251. In addition, when a certain biological sample 200 is returned, the management server 2 deletes the corresponding extraction record.
In this embodiment, the management server 2 also stores the personal information. That is, the related information of the researchers and the managers is stored, including the identification information, the personal information (name, department, etc.) and the identity information of each of the researchers and the managers. The identity information represents whether the person is a scientific research person or an administrator. The management server 2 determines whether the identification information corresponds to the scientific research personnel or the administrator based on the stored personnel information, and further determines whether the scientific research personnel has the door opening authority when determining that the scientific research personnel exists.
The judgment of whether the scientific research personnel have the door opening permission is carried out based on the identification information, the extraction record and the request record, and the specific process is as follows:
when receiving the identification information, the management server 2 retrieves the request records respectively according to the received identification information and the cabinet number, determines whether the matched request records exist, and further determines whether the cabinet number exists in the sample position information of the matched request records when the matched request records exist; when retrieving the extracted record, the management server 2 retrieves the extracted record according to the received identification information and determines whether a matched extracted record exists, further acquires corresponding storage requirement information from the sample related information according to the sample identification information in the extracted record when the matched extracted record exists, and determines whether the cabinet number exists in the acquired storage requirement information.
Once the final result of any one of the two determinations is that the cabinet exists (that is, the transmitted cabinet number exists in the sample position information requested to be recorded or the cabinet number exists in the storage requirement information), the management server 2 sends a door opening permission signal to the sample cabinet 1, so that the main control chip 18 controls the locking part 13 to unlock according to the door opening permission signal, thereby enabling the scientific research staff to open the cabinet door 12. The door opening permission signal comprises a layer number, and the layer number is the layer number in the request record under the condition that the request records are matched; in the case where the extraction records match, the tier number is one or more tier numbers corresponding to the cabinet number in the storage requirement information.
If neither of the above determinations is determined to be present, no response is given (alternatively, the management server 2 may send a warning signal to the corresponding sample cabinet 1 to cause the warning unit 17 to issue a warning to remind the scientific research staff, where the warning may be in the form of a short red light emitted by the warning unit 17).
In step S4, the main control chip 18 controls the corresponding locking portion 13 to unlock the cabinet door 12 according to the layer number in the door opening permission signal, so that the scientific research staff can open the cabinet door 12 to perform the operation of picking up or storing. Once the scientific researcher opens the cabinet door 12 so that the sensing part 14 senses that the cabinet door 12 is opened, the process proceeds to step S5. If the sensing part 14 does not sense that the cabinet door 12 is opened within a predetermined time, the control locking part 13 locks the cabinet door 12 again.
In step S5, the main control chip 18 controls the determination unit 15 to continuously perform the reading operation and temporarily store the determination information and the number of times of the read sample, and once the scientific research personnel complete the extraction or storage and close the cabinet door 12, so that the sensing unit 14 senses that the cabinet door 12 is closed, the process proceeds to step S6. In addition, if the closing of the cabinet door 12 is not sensed after the predetermined time, the main control chip 18 controls the warning part 17 to give a warning (for example, continuously give a red light, etc.), thereby prompting the scientific research personnel to close the cabinet door 12 in time.
In step S6, the main control chip 18 controls the locking part 13 to lock the cabinet door 12, controls the sample cabinet communication module to send the identification number, the cabinet number, the layer number corresponding to the determination part 15, and all the sample determination information and the read times thereof to the management server 2, and then proceeds to step S7.
In this embodiment, the main control chip 18 controls the determination unit 15 to continuously perform the reading action after the sensing unit 14 senses that the cabinet door 12 is opened, so that the sample tag 201 can be read by the determination unit 15 when the biological sample 200 is taken and placed; meanwhile, the main control chip 18 also temporarily stores all the sample discrimination information read during the continuous reading operation and the number of times of reading. Then, when the sensing portion 14 senses that the cabinet door 12 is closed again, the main control chip 18 controls the sample cabinet communication module to transmit the identification number, the cabinet number, the layer number corresponding to the determination portion 15, and all the read sample determination information and the read times thereof to the management server 2 while controlling the locking portion 13 to lock the cabinet door 12.
In step S7, the management server 2 determines the access status of the biological sample 200 according to the identification number, the cabinet number, the layer number sample discrimination information and the reading frequency sent from the sample cabinet 1, updates the sample position information in the sample-related information, and updates the extraction record and the request record, and then proceeds to step S8.
The management server 2 determines access to the biological sample 200 based on the transmitted information, and updates the sample position information in the sample-related information. That is, when the same sample discrimination information is read for an odd number of times, it is first determined whether the cabinet number and the layer number already exist in the sample position information corresponding to the sample discrimination information, and if so, it is determined that the biological sample 200 is taken out, and the sample position information corresponding thereto is rewritten to "taken out" or similar contents indicating that it has been taken out; if there is no cabinet number and layer number (i.e., it has been marked as "taken out"), it is determined that the biological sample 200 is placed, and at this time, the corresponding sample position information is rewritten into the cabinet number and layer number corresponding to the determination section 15 (which can be obtained from the information sent from the sample cabinet 1), and further added to the sample position information based on the position information (for example, the left, right, or middle area) read by the determination section 15. In addition, when the same sample discrimination information is read even times, it is considered that the corresponding biological sample 200 is taken out and put back or taken out after being put in, and at this time, rewriting of the corresponding sample position information is not performed.
In addition, when a certain biological sample 200 is determined to be extracted, the management server 2 stores the corresponding sample discrimination information and the identification information received from the sample cabinet 1 as one extraction record, and deletes the corresponding request record based on the corresponding sample discrimination information and the received identification information.
In step S8, the management server 2 further determines whether the biological sample 200 extracted by the researcher is correct or not according to the access condition determination result and the extraction record, determines whether the position where the biological sample 200 is stored is correct or not according to the access condition determination result and the storage requirement information, and proceeds to step S9 when it is determined that the researcher has an extraction error or a storage error, and proceeds to an end state when it is determined that there is no error.
In this embodiment, once the determination of the access condition of the biological sample 200 is completed, the access condition may include the extracted biological sample 200 or the deposited biological sample 200.
When a certain biological sample 200 is determined to be extracted, the management server 2 acquires a corresponding biological sample name from the request record as an extraction target name based on the identification information, acquires a corresponding biological sample name from the sample-related information as an actual extraction target name based on the received biological sample discrimination information, further determines whether the extraction target name is identical to the actual extraction target name, and determines that the scientific researcher has extracted an erroneous sample if the extraction target name is not identical to the actual extraction target name.
When a certain biological sample is judged to be stored, the management server 2 retrieves and acquires corresponding storage requirement information from the sample-related information according to the sample identification information, further judges whether the cabinet number and the layer number for storing the biological sample exist in the acquired storage requirement information, and judges that the biological sample 200 is placed at an incorrect position by the scientific research personnel when the cabinet number and the layer number for storing the biological sample do not exist.
In step S9, the management server 2 sends a warning signal to the corresponding sample cabinet 1, so that the warning portion 17 activates a warning to remind the scientific research personnel that the wrong sample is taken out (for example, continuously emitting red light at a specific light emitting frequency), and then enters an end state.
In this embodiment, when the researcher needs to return, the corresponding extraction record received from the management server 2 may be displayed through the human-computer interaction screen of the terminal 3 of the researcher, and the biological sample 200 that needs to be returned is selected from the extraction record. Then, the management server 2 sends the corresponding storage requirement information back to the scientific research personnel terminal 3 according to the selected biological sample 200, so that the scientific research personnel can check the storage requirement information through the scientific research personnel terminal 3 and go to the corresponding sample cabinet to return according to the storage requirement information.
Examples effects and effects
According to the biological sample management method for scientific research provided by the embodiment, the sample labels are arranged on the biological samples, and the sample labels are distinguished in the distinguishing part of the sample cabinet, so that the biological labels on the biological samples are distinguished after the cabinet door is opened by scientific research personnel, and the corresponding biological sample distinguishing information is obtained, so that the management server can judge the access condition of the biological samples according to the biological sample distinguishing information and update and process the sample position information, therefore, the positions of the biological samples can be effectively obtained and timely updated and stored, and the access operation in the subsequent use process is facilitated.
In addition, because the opening and closing of the induction part induction cabinet door is adopted, the judgment part is allowed to judge when the cabinet door is opened, so that the sample labels can be continuously read under the states that the cabinet door is closed and the sample cabinet is not used, the working time of the judgment part is shortened, the service life of the judgment part is prolonged, and the problem that the reading work of the cabinet door on the judgment part is influenced due to the fact that the cabinet door is read under the condition that the cabinet door is closed is also avoided. For example, if the reading is performed when the cabinet door is closed, the sample label is not easily read by the determination part due to the blockage of the cabinet door; however, if the cabinet door is arranged on the inner side of the cabinet door, the temperature in the placing layer is too low to work normally.
In the embodiment, the storage requirement information of each biological sample is stored, and meanwhile, the management server can judge whether the wrong position is placed according to the storage requirement information, and the warning part on the sample cabinet directly gives out a warning when the wrong position is placed, so that the sample cabinet or the placement layer which does not accord with the storage requirement can be effectively prevented from being placed when the biological sample is returned.
Moreover, since the extraction request and the extraction record are stored, after the identification part of the sample cabinet identifies, the management server can search the extraction record and the request record according to the identification information and the cabinet number to judge whether the sample cabinet has the authority to open the sample cabinet, and the management-side control part controls the management-side communication part to send the door opening permission signal when the management-side communication part has the authority to open the sample cabinet, so that the management method of the embodiment can unlock the cabinet door when the scientific research personnel really needs to access the cabinet, and avoids the influence of mistaken opening on the low-temperature environment in the placing layer. Moreover, in the embodiment, the sent door opening permission signal further includes a layer number in the extraction request record or a layer number corresponding to the cabinet number in the storage requirement information, so that the sample cabinet control module can control the locking part of the corresponding placement layer to unlock according to the layer number, and further, the phenomenon that a scientific research worker opens a wrong placement layer to influence the temperature of the placement layer when taking and placing the sample cabinet is avoided.
In addition, in the embodiment, after the access condition is judged, the management server can acquire the name to be extracted according to the identification information, acquire the actual extracted name according to the biological sample judgment information, and judge whether the two names are consistent, so that whether the scientific research personnel extract the wrong biological sample or not can be judged, the warning part starts warning to remind the scientific research personnel, a warning prompt effect is formed, and the scientific research personnel return the biological sample which is taken out by mistake. Since the management server updates the position information after judging the access condition, the extraction record is formed, and under the condition of mistaken extraction, when the scientific research personnel use the identification part to read the identification information, the opening authority can be judged according to the extraction record so as to open the corresponding cabinet door, so that even under the condition of mistaken extraction, the scientific research personnel can smoothly return.
The above-described embodiments are merely illustrative of specific embodiments of the present invention, and the present invention is not limited to the description of the above-described embodiments.
For example, in the above embodiment, the management server can check and confirm whether or not the extraction is correct based on the access determination result and the extraction record, and determine whether or not the storage is correct based on the storage access determination result and the storage request information. In other schemes of the invention, in order to ensure that the biological sample which is stored by mistake can be returned smoothly, the misplaced record can be stored in the management server, and the misplaced record is used for storing the sample discrimination information which is stored by mistake, the stored position information and the identification information of the researcher which is stored by mistake as the misplaced record under the condition of judging the storage mistake, so that when the researcher operates the identification part, the management server can judge the opening authority according to the request record and the extraction record, and can judge the opening authority according to the misplaced record, and the researcher who is stored by mistake can take out the misplaced biological sample and store the misplaced biological sample to a proper position.
In the above embodiment, the RFID reader of the sample cabinet is mounted on the layer eaves portion, and the reading direction of the RFID reader faces outward, so that the sample tag can be read when leaving or entering the placement layer, and the access judgment and update portion performs corresponding access judgment and update.
However, in another aspect of the present invention, an installation manner of directing the reading direction of the RFID reader to the inside may be adopted, for example, a form of a directional RFID reader is adopted, the directional RFID reader is installed to be slightly inclined inward, and the reading direction of the directional RFID reader is immediately directed to the inside, and at the same time, a slope portion inclined inward may be provided at the eaves portion of the layer where the RFID reader is located, so that the electromagnetic wave of the RFID reader enters the placing layer to read the sample tag on the biological sample therein. In the embodiment, the sample cabinet control module controls the judging part to continuously read and temporarily store the sample judging information contained in the read sample labels according to the opening condition of the cabinet door sensed by the sensing part when the cabinet door is opened, and sends the finally read sample judging information, the layer number and the cabinet number of all the sample labels to the management server after the cabinet door is closed, and at the moment, the sample judging information corresponds to the biological sample which is actually present in the layer and is currently placed after the scientific research personnel finishes taking and placing actions. Then, the management server obtains all corresponding sample discrimination information (equivalent to the biological samples stored in the placing layer before the taking and the placing) from the sample related information according to the cabinet number and the layer number, and then compares the sample discrimination information with the sample discrimination information of the biological samples actually existing in the current placing layer sent by the sample cabinet, so that the taken or placed biological samples can be judged, and the sample related information is updated.
The method in the embodiment is implemented based on a sample cabinet composed of a plurality of storage levels, and therefore the locking portion, the sensing portion, and the determination portion correspond to each storage level, and the transmitted door opening permission signal contains a level number, and the position information also contains the level number. However, the method of the present invention may be implemented based on a sample cabinet having only one placement floor, and only one of the locking portion, the sensing portion, and the determination portion needs to be provided, and in this case, the door opening permission signal may include only the cabinet number, and the position information may include only the cabinet number and the approximate position (left side, middle portion, or right side) in the cabinet.
In addition, the RFID reader of the embodiment is a directional reader, so that the RFID reader can be installed towards the outer side, and the purpose of reading in the placing layer is not achieved. However, the RFID reader of the present invention may also be a common non-directional RFID reader, and when the RFID reader is mounted, the non-directional RFID reader is embedded in the interlayer board, and a certain shielding layer is disposed at an inner side of the non-directional RFID reader (for example, a metal plate is attached to the inner side of the non-directional RFID reader as a shielding layer), so that electromagnetic waves can be prevented from entering the placing layer during reading, and the reading direction faces outward.
In addition, the interlayer plate of the embodiment is provided with a shielding layer so as to prevent an RFID reader from reading the labels on the biological samples of other layers. As a further embodiment, in the present invention, a shielding layer may be further disposed at an outer side position of each directional RFID reader, so as to prevent the reading range of the RFID reader from extending outward too much, in this case, the reading range of each RFID reader is only equivalent to a region vertically below the reading range, so that repeated reading (for example, the biological sample is taken by a scientific researcher during taking and placing or is temporarily placed on the taking and placing tray and thus is located at the position) when the biological sample is located at an outer side position close to the placement layer can be avoided, and thus the moving condition of the sample label in and out of the placement layer can be more accurately read.
For example, in the above embodiment, the warning portion is a warning light disposed on the front surface of the cabinet door. In another aspect of the present invention, the warning unit may be an audible and visual alarm or other device having a prompt effect, and may be disposed at a position where the reagent tank is easily viewed or where sound is easily emitted, as required.
For example, in the above-described embodiment, the sample-related information includes the sample discrimination information, the corresponding sample name, and the sample position information and the storage request information. In other embodiments of the present invention, the sample-related information may also include other sample-related information, for example, other related information such as a sampling source and a sampling time of the biological sample may be correspondingly stored.
Claims (9)
1. A biological sample management method for scientific research is characterized in that a system comprising a sample cabinet, a scientific research personnel terminal and a management server is adopted to realize the management of biological samples with sample labels, and comprises the following steps:
correspondingly storing the sample discrimination information, the corresponding sample name, the sample position information and the storage requirement information in the management server as sample related information;
identifying the identification information of scientific research personnel by adopting an identification part of the sample cabinet, and then sending the identification information to the management server;
judging whether the scientific research personnel have the opening authority or not at least based on the identification information by adopting the management server, and sending an opening permission signal to the sample cabinet once the scientific research personnel have the opening authority;
the control module of the sample cabinet unlocks a cabinet door according to the door opening permission signal, so that the scientific research personnel can open the cabinet door to perform extraction or storage operation;
adopting a judging part to continuously read and adopting a sensing part to sense whether the cabinet door is closed or not, and sending the read sample judging information and the cabinet number of the sample cabinet to the management server once sensing that the scientific research personnel closes the cabinet door;
the management server judges the access condition of the biological sample according to the transmitted sample distinguishing information and the cabinet number, and at least updates the sample position information in the sample related information.
2. The method for managing biological samples for scientific research as set forth in claim 1, wherein:
wherein the management server updates the sample position information in the sample-related information and stores the sample discrimination information corresponding to the biological sample determined to be extracted and the identification information received from the sample cabinet as one extraction record.
3. The method for managing biological samples for scientific research as set forth in claim 2, wherein:
wherein the scientific research personnel inputs the name of the biological sample to be extracted as the name of the required sample through the terminal of the scientific research personnel and sends the name of the required sample to the management server through the terminal of the scientific research personnel,
and the management server retrieves the consistent sample discrimination information from the sample related information according to the received required sample name, so as to further obtain the sample position information, and sends the retrieved sample position information and the received required sample name as extraction information to a scientific research personnel terminal.
4. The method for managing biological samples for scientific research as set forth in claim 3, wherein:
the management server sends the extraction information to a scientific research personnel terminal and stores the name of the required sample, the extraction information and the identification information of the scientific research personnel received from the scientific research personnel terminal as a request record.
5. The method for managing biological samples for scientific research as set forth in claim 4, wherein:
the management server judges whether scientific research personnel have the opening authority according to the extraction record, the request record and the received identification information, and sends an opening permission signal to the sample cabinet once judging that the scientific research personnel have the opening authority.
6. The method for managing biological samples for scientific research as set forth in claim 5, wherein:
the management server judges whether scientific research personnel have the opening authority and carries out the process based on the extraction record and the request record, and the specific process is as follows:
respectively retrieving the request records according to the received identification information and the cabinet number, judging whether the matched request records exist, and further judging whether the cabinet number exists in the sample position information of the matched request records when the matched request records exist;
retrieving the extracted records according to the received identification information, judging whether the matched extracted records exist, further acquiring corresponding storage requirement information from the sample related information according to sample identification information in the extracted records when the matched extracted records exist, and judging whether the sent cabinet number exists in the acquired storage requirement information,
and once the final result of any one judgment is existence, judging that the scientific research personnel has the opening authority.
7. The scientific biological sample management method as set forth in claim 2, further comprising:
the management server further judges whether the biological sample extracted by the scientific research personnel is correct or not according to the judgment result of the access condition and the extraction record, and simultaneously judges whether the position where the biological sample is stored is correct or not according to the judgment result of the access condition and the corresponding storage requirement information,
and when the scientific research personnel are judged to have extraction errors or storage errors, the management server sends a warning signal to the corresponding sample cabinet.
8. The method for managing biological samples for scientific research as set forth in claim 7, wherein:
wherein, the process of judging whether the extracted biological sample is correct is as follows:
and acquiring the corresponding biological sample name from the extraction record according to the identification information as an extraction name, acquiring the corresponding biological sample name from the sample related information according to the received biological sample discrimination information as an actual extraction name, further judging whether the extraction name is consistent with the actual extraction name, and judging that the scientific research personnel has an extraction error if the extraction name is not consistent with the actual extraction name.
9. The method for managing biological samples for scientific research as set forth in claim 7, wherein:
wherein, the process of judging whether the stored biological sample is correct is as follows:
and retrieving and acquiring the corresponding storage requirement information from the biological sample related information according to the sample discrimination information, further judging whether the position information at least comprising the cabinet number of the stored biological sample exists in the acquired storage requirement information, and judging that the scientific research personnel has a storage error when the position information does not exist.
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