CN113128632A - Intelligent management method for non-immobilized biological reagent - Google Patents

Abstract

The invention provides an intelligent management method of a non-fixed biological reagent, which can effectively manage the biological reagent needing to be frozen and stored, and comprises the following steps: correspondingly storing the reagent name, corresponding reagent identification information and reagent position information of each biological reagent in a management server as reagent related information; a laboratory technician terminal is adopted to enable a laboratory technician to input a name of a required reagent and send the name to a management server; retrieving corresponding extracted information and sending the extracted information back to the experimenter terminal; the laboratory technician terminal displays the received extraction information, and the laboratory technician goes to a corresponding low-temperature reagent cabinet according to the extraction information; adopting an identification part for identification; judging whether the experimenter has the authority to open the cabinet door or not by adopting a management server at least based on the identification information; unlocking the cabinet door to allow a laboratory worker to open the cabinet door for extraction or storage operation; continuously judging reagent judgment information contained in all reagent labels in the reagent cabinet by adopting a judgment part and sending the reagent judgment information to a management server; and updating the reagent related information.

Description

Intelligent management method for non-immobilized biological reagent

Technical Field

The invention relates to an intelligent management method for non-immobilized biological reagents.

Background

The biological reagent is a common biological sample or organic compound in a laboratory, and needs to be stored in different ways according to the properties of different biological reagents, and generally speaking, the biological reagent can be properly stored in different low-temperature or normal-temperature environments according to the required holding temperature.

In a laboratory, different biological reagents are generally classified and stored by adopting a refrigerated cabinet with a preset temperature, and a laboratory worker can go to the refrigerated cabinet with a corresponding temperature to find the required biological reagents when needed.

However, this storage method has a problem of such management confusion. For example, when a large amount of biological reagents are stored in the refrigerator, the laboratory technician will certainly spend a long time searching for the biological reagents in the refrigerator, which not only easily causes the leakage of cold air, but also easily exposes the laboratory technician to a cold environment for a long time, and especially, such adverse effects are more easily generated when the refrigerating temperature of the refrigerator is low. If a strict reagent management method is adopted to solve these problems so that the reagents are stored in order, it is inevitable that the recovery, management, and the like are troublesome.

Meanwhile, when the reagent is returned, the biological reagent is stored in the wrong environment due to the fact that the biological reagent is prone to being placed wrongly and the like, so that the biological reagent is damaged and scrapped, and even the experiment is affected.

Disclosure of Invention

In order to solve the problems, the invention provides an intelligent management method of non-fixed biological reagents, which can conveniently and effectively manage the biological reagents which need to be preserved at different temperatures in a laboratory, and adopts the following technical scheme:

the invention provides an intelligent management method of non-immobilized biological reagents, which is characterized in that a system comprising a low-temperature reagent cabinet for storing biological reagents needing low-temperature environment freezing storage, a laboratory terminal and a management server is adopted to realize the management of the biological reagents with reagent labels in a laboratory, and the intelligent management method comprises the following steps: correspondingly storing the reagent name of each biological reagent, the corresponding reagent identification information and corresponding reagent position information comprising the cabinet number of the low-temperature reagent cabinet where the reagent bottle is located and the layer number of the placed layer in the management server as reagent related information; enabling the experimenter to input the name of the biological reagent to be extracted as a required reagent name by adopting the experimenter terminal and sending the required reagent name to the management server; the management server retrieves the reagent related information according to the sent required reagent name, acquires corresponding reagent position information, and sends the acquired reagent position information and the required reagent name serving as extraction information to the laboratory technician terminal; the laboratory technician terminal displays the received extraction information to allow the laboratory technician to go to the corresponding low-temperature reagent cabinet according to the extraction information; identifying the identification information of the experimenter by adopting an identification part of the low-temperature reagent cabinet, and sending the identification information to the management server for judgment; judging whether the laboratory technician has the authority to open the cabinet door or not at least based on the identification information by adopting the management server, and sending a door opening permission signal to the low-temperature reagent cabinet once the laboratory technician has the authority to open the door; the control module of the low-temperature reagent cabinet unlocks a cabinet door according to the door opening permission signal so as to enable the experimenter to open the cabinet door for extraction or storage operation; adopting a judging part to continuously judge reagent judging information contained in all reagent labels in the reagent cabinet and adopting a sensing part to sense whether the cabinet door is closed, and sending the read reagent judging information, the current cabinet number of the low-temperature reagent cabinet and the layer number of the corresponding placing layer to the management server once sensing that the laboratory technician closes the cabinet door; the management server judges the access condition of the biological reagent and updates the reagent-related information at least according to the transmitted reagent judgment information, the cabinet number and the layer number.

Action and Effect of the invention

According to the intelligent management method of the non-fixed biological reagent, the reagent labels are arranged on the reagent bottles in the laboratory, and the distinguishing parts capable of distinguishing the reagent labels are correspondingly arranged on the placing layers of the reagent cabinet, so that after the sensing parts on the reagent cabinet sense that a laboratory worker opens the cabinet door, the distinguishing parts distinguish the change of the reagent distinguishing information of the reagent bottles corresponding to the placing layers, the management server can update the states of the reagent positions and the like according to the reagent distinguishing information, and the accurate management of the reagent bottles aiming at each biological reagent is realized. In addition, because the cabinet number of the reagent cabinet where each reagent bottle is located and the layer number of the corresponding placing layer are recorded, when a laboratory technician needs to extract the biological reagent, the position of the required biological reagent can be easily and quickly found, and therefore the management effect that the position of each reagent bottle can be accurately grasped even if the laboratory technician can conveniently and randomly place the reagent bottle in the reagent cabinet is realized. Further, since the laboratory technician is required to return to any position of the reagent tank after use, the biological reagent is kept at a predetermined low temperature as much as possible, thereby suppressing the deterioration of the original performance.

Drawings

FIG. 1 is a system configuration diagram of a method for intelligent management of an unfixed biological reagent according to one embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a reagent cabinet according to a first embodiment of the present invention;

FIG. 3 is a schematic view showing the placement of a reagent bottle according to a first embodiment of the present invention;

FIG. 4 is a functional block diagram of a reagent cabinet according to a first embodiment of the present invention;

FIG. 5 is a diagram illustrating the contents stored in the bin number layer number storage unit according to an embodiment of the present invention;

FIG. 6 is a flow chart of a method for intelligent management of non-immobilized biological agents in accordance with one embodiment of the present invention;

FIG. 7 shows the contents of reagent-related information in an embodiment of the present invention;

FIG. 8 is a diagram illustrating the content of the extracted information according to the first embodiment of the present invention;

FIG. 9 is a diagram illustrating content requested for recording in accordance with one embodiment of the present invention;

FIG. 10 is a diagram illustrating the content of an extraction record according to an embodiment of the present invention;

FIG. 11 is a schematic structural diagram of a reagent cabinet according to a second embodiment of the present invention; and

FIG. 12 is a system configuration diagram of a method for intelligently managing an unfixed biological reagent in the third embodiment of the present invention.

Detailed Description

Hereinafter, the experimental-based intelligent management method for non-immobilized bioreagent according to the present invention will be described in detail with reference to the accompanying drawings.

In one embodiment, the present invention provides an intelligent management method for non-immobilized biological reagents, which is characterized in that a system including a low-temperature reagent cabinet for storing biological reagents requiring low-temperature environmental freezing storage, a laboratory terminal and a management server is used to manage biological reagents having reagent labels in a laboratory, and the method includes the following steps: correspondingly storing the reagent name of each biological reagent, the corresponding reagent identification information and corresponding reagent position information comprising the cabinet number of the low-temperature reagent cabinet where the reagent bottle is located and the layer number of the placed layer in the management server as reagent related information; enabling the experimenter to input the name of the biological reagent to be extracted as a required reagent name by adopting the experimenter terminal and sending the required reagent name to the management server; the management server retrieves the reagent related information according to the sent required reagent name, acquires corresponding reagent position information, and sends the acquired reagent position information and the required reagent name serving as extraction information to the laboratory technician terminal; the laboratory technician terminal displays the received extraction information to allow the laboratory technician to go to the corresponding low-temperature reagent cabinet according to the extraction information; identifying the identification information of the experimenter by adopting an identification part of the low-temperature reagent cabinet, and sending the identification information to the management server for judgment; judging whether the laboratory technician has the authority to open the cabinet door or not at least based on the identification information by adopting the management server, and sending a door opening permission signal to the low-temperature reagent cabinet once the laboratory technician has the authority to open the door; the control module of the low-temperature reagent cabinet unlocks a cabinet door according to the door opening permission signal so as to enable the experimenter to open the cabinet door for extraction or storage operation; adopting a judging part to continuously judge reagent judging information contained in all reagent labels in the reagent cabinet and adopting a sensing part to sense whether the cabinet door is closed, and sending the read reagent judging information, the current cabinet number of the low-temperature reagent cabinet and the layer number of the corresponding placing layer to the management server once sensing that the laboratory technician closes the cabinet door; the management server judges the access condition of the biological reagent and updates the reagent-related information at least according to the transmitted reagent judgment information, the cabinet number and the layer number.

In the above-described embodiment, the technical feature may be that the determination unit continuously determines the reagent determination information included in all the reagent labels in the reagent kit on the basis of at least two determination units having different part numbers provided for each of the placement layers, and specifically includes: sending the read reagent identification information, the current cabinet number of the low-temperature reagent cabinet, the layer number of the corresponding placement layer and the part number corresponding to the identification part to the management server; the management server also judges rough position information corresponding to the reagent discrimination information according to the received reagent discrimination information and the layer number; and when updating the reagent position information in the reagent related information, the management server also updates the reagent related information according to the rough position information.

In the above-described embodiment, there may be further provided a technical feature that, when the laboratory technician terminal displays the received extraction information, the laboratory technician terminal also displays the rough location information so as to allow the laboratory technician to search for the desired biological reagent at the corresponding rough location in the corresponding placement layer based on the rough location information.

In the above aspect, the management server may further include: comparing the received reagent discrimination information with the reagent discrimination information in the reagent-related information to discriminate a missing reagent discrimination information corresponding to the taken-out biological reagent or an added reagent discrimination information corresponding to the returned biological reagent; and updating the reagent-related information according to the missing reagent discrimination information or the added reagent discrimination information. In the above-described embodiment, the management server may be further configured to, when determining the additional reagent discrimination information, determine whether or not the corresponding biological reagent is placed in a wrong reagent tank based on the additional reagent discrimination information and the corresponding tank number.

In the above-described embodiment, the reagent management server may further include a storage temperature range corresponding to a required storage temperature of the biological reagent, and the management server may determine whether or not the biological reagent is placed in an incorrect reagent rack based on the storage temperature range and current temperature information corresponding to the low-temperature environment of the reagent rack, and specifically include: judging whether the current temperature information corresponding to the cabinet number is in the storage temperature range corresponding to the added reagent judgment information; if the current temperature information is judged to be in the storage temperature range, judging that the biological reagent is not placed in a wrong reagent cabinet; and if the current temperature information is not within the storage temperature range, the biological reagent is placed in the wrong reagent cabinet, and the management server sends a warning signal to the corresponding low-temperature reagent cabinet.

In the above-described embodiment, the management server may further store, as one extraction record, reagent identification information corresponding to the biological reagent determined to be extracted and identification information received from the low-temperature reagent cabinet while updating the reagent-related information, and the management server may determine whether or not the biological reagent is placed in an incorrect reagent cabinet based on the extraction record, and specifically include: judging whether the biological reagent is put into a wrong reagent cabinet according to the fact that whether the received cabinet number is consistent with the reagent position information corresponding to the reagent identification information in the extraction record, and judging that the biological reagent is not put into the wrong reagent cabinet if the cabinet number is consistent with the reagent position information; and if the cabinet number is judged to be inconsistent with the reagent position information, judging that the biological reagent is placed in the wrong reagent cabinet, and sending a warning signal to the corresponding low-temperature reagent cabinet by the management server.

< example one >

FIG. 1 is a system configuration diagram of an intelligent management method for an unfixed biological reagent in the embodiment of the present invention.

As shown in fig. 1, the intelligent management method for non-immobilized biological reagents is implemented by using a system 100, and the system 100 includes j reagent cabinets 1, a management server 2, a plurality of laboratory staff terminals 3, and communication networks 4a and 4 b.

The management server 2 is respectively connected with the reagent cabinets 1 in a communication way through a communication network 4a, and is respectively connected with the laboratory technician terminals 3 in a communication way through a communication network 4 b. In this embodiment, the communication networks 4a and 4b are both local area networks in a laboratory.

The reagent cabinet 1 is used for storing reagent bottles which are filled with biological reagents required to be stored in a low-temperature environment in a laboratory, wherein the biological reagents are various biological materials, organic compounds or reagents for clinical and medical research.

Fig. 2 is a schematic structural diagram of a medium-low temperature reagent cabinet according to an embodiment of the present invention.

As shown in fig. 2, the low-temperature reagent tank 1 includes a tank body 101, a temperature adjustment unit 102, five placement levels 103 located in the tank body, ten determination units 104 provided corresponding to the placement levels 103, a tank door 105 provided in the tank body, a locking unit 106, a sensing unit 107, an identification unit 108, a warning unit 109, and a main control chip 110.

The temperature adjustment unit 102 adjusts the temperature inside the reagent tank 1 to form a low-temperature environment suitable for storing biological reagents.

In this embodiment, the low temperature environment manufactured by the temperature adjusting portion 102 is between-10 ℃ and-30 ℃, the temperature adjusting portion 102 includes a cooling mechanism such as a compressor, a temperature setting mechanism for setting a temperature, a temperature detecting mechanism for detecting a temperature in the placing layer 103, and a control mechanism for controlling the operation of the cooling mechanism according to a temperature detection result and the set temperature so as to realize temperature control, and specific configurations and operating principles of these mechanisms are the same as those in the ultra-low temperature refrigerator in the prior art, and are not described herein again.

The placing layer 103 is provided with five layers for placing various reagent bottles. In the low-temperature reagent tank 1, a first layer accommodating layer 103a, a second layer accommodating layer 103b, a third layer accommodating layer 103c, a fourth layer accommodating layer 103d, and a fifth layer accommodating layer 103e are arranged in this order from top to bottom. Wherein, each layer of the placing layer 103 has a placing plate on which the reagent bottles are placed (the placing plate of the fifth layer of the placing layer 103e is the bottom plate of the reagent cabinet 1).

FIG. 3 is a schematic view of a reagent bottle according to a first embodiment of the present invention.

Taking the first-layer placing layer 103a as an example, as shown in fig. 3, the reagent bottles 150 can be freely placed on the placing plate 151. A reagent label 151 is attached to the cap and body of the reagent bottle 150.

In this embodiment, the reagent label 151 is a low temperature resistant RFID label that contains reagent identification information corresponding to the reagent bottle 150, and can be installed at any position of the reagent bottle 150, such as a bottle cap, a bottle body, or a bottle bottom, and can normally operate at least in a low temperature environment of-30 ℃.

The determination unit 104 is an RFID reader operable at least in a low temperature environment of-30 ℃ and is configured to determine the reagent labels 151 of all the reagent bottles 150 on the respective placement layers 103 in the reagent tank 1.

In this embodiment, two determination portions 104 are provided for each placement layer 103, and as in the first placement layer 103a, the determination portions 104a-1 and 104a-2 are provided symmetrically on the left and right sides of the placement layer 103 a.

The cabinet door 105 corresponds to the reagent cabinet 1, and the laboratory technician needs to open the cabinet door 105 first to extract or return the biological reagents on each placing layer 103 of the reagent cabinet 1.

The locking portion 106 includes an electronic lock 161 provided at an edge of the cabinet door and a locker 162 provided at the door frame. The electronic lock 131 can lock and unlock the cabinet door 12 according to the control signal of the main control chip 19 under the cooperation of the lock catch 162.

The sensing part 107 is a sensor provided on the cabinet door, and can sense whether the cabinet door 105 is in a closed state.

The identification unit 108 is a door card reader for identifying a door opening card held by an experimenter.

In this embodiment, the door opening card includes identification information of an experimenter, and the identity recognition portion 108 enables the experimenter to recognize the identification information corresponding to the experimenter in the door opening card in a card swiping manner. Each experimenter has respective identification information, and the identity recognition part 108 can correspondingly judge the identity of the experimenter through the identification information.

The warning part 109 is a warning lamp arranged on the front surface of the cabinet door 105, and is used for generating light flicker when receiving warning information so as to prompt staff to take or return wrong biological reagents.

Fig. 4 is a functional block diagram of a medium and low temperature reagent cabinet according to an embodiment of the present invention.

As shown in fig. 4, the main control chip 110 is electrically connected to the temperature adjustment unit 103, the determination unit 104, the locking unit 106, the sensing unit 107, the identification unit 108, and the warning unit 109, respectively, and controls operations of these components. The main control chip 110 includes a reagent tank communication unit 1101 and a tank number layer number storage unit 1102.

The reagent tank communication unit 1101 is a communication module of the main control chip 110, and is used for performing data communication between the main control chip 110 and the management server 2.

When the identification information of the laboratory technician is identified by the identification identifying unit 108, the reagent cabinet communication unit 1101 transmits the identification information and information such as the current cabinet number of the reagent cabinet 1 to the management server 2 for processing, and when the reagent cabinet communication unit 1101 receives the door opening permission signal fed back from the management server 2, the main control chip 110 controls the locking unit 106 to unlock, so that the laboratory technician opens the cabinet door 105 to extract or return the biological reagent. In addition, when the sensing part 107 senses that the cabinet door 105 is changed from the open state to the closed state, the main control chip 110 controls the locking part 106 to lock the cabinet door.

Fig. 5 shows the contents stored in the cabinet number layer number storage unit according to the first embodiment of the present invention.

In this embodiment, in order to distinguish the reagent cabinets 1, each reagent cabinet 1 in the laboratory is provided with a cabinet number for identification. Meanwhile, in each reagent cabinet 1, the respective placing layers 103 are also provided with corresponding layer numbers in sequence, for example, the layer number of the first placing layer 103a is 1. As shown in fig. 5, the cabinet number/layer number storage unit 1102 stores the cabinet number 1103 of the current reagent kit 1, the layer number 1104 of each of the set layers 103, and the determination unit number 1105 of the determination unit 104 (i.e., the part number of the determination unit 104) corresponding to each of the layer numbers 1104, and two determination unit numbers 1105 are associated with each of the layer numbers 1104 because two determination units 104 are provided for each of the set layers 103. In practical applications, the cabinet number is the chip identification code of the main control chip 110, and the identification part number is the machine code of each identification part 104.

When the laboratory technician opens and closes the cabinet door 105 when taking out or returning the reagent, the sensing unit 107 senses the open and closed state of the cabinet door, and at this time, the main control chip 110 controls the determination unit 104 to determine the reagent determination information corresponding to all the reagent bottles 150 on each placement layer 103, acquires the corresponding layer number 1104 and cabinet number 1103 from the cabinet number layer number storage unit 1102 according to the determination unit number 1105 of the determination unit 104, and further controls the reagent cabinet communication unit 1101 to transmit the reagent determination information, the corresponding layer number, cabinet number, and determination unit numbers of the determination units to the management server 2 in correspondence after controlling the locking unit 106 to lock the cabinet door.

In this embodiment, since the determination unit 104 is disposed in the placement layer 103, the main control chip 110 controls the determination unit 104 to perform the determination when the sensing unit 107 senses that the cabinet door is opened and then closed again.

The management server 2 is used for storing and managing biological reagents, i.e., information related thereto.

The experimenter terminal 3 is in communication connection with the management server through a communication network 4, and is used for enabling an experimenter to perform corresponding human-computer interaction including information input, information viewing and the like.

FIG. 6 is a flow chart of a method for intelligent management of non-immobilized bioreagent in an embodiment of the present invention.

As shown in fig. 6, the intelligent unfixed biological reagent management method of the present embodiment is implemented based on the system 100 of the reagent cabinet 1, the management server 2, the laboratory technician terminal 3 and the communication network 4, and is used for managing information of the reagent bottle 150 storing a biological reagent based on the reagent tag 151 formed by an RFID tag, and specifically includes the following steps:

in step S1, the reagent discrimination information, the corresponding reagent name, and the reagent position information are stored as the reagent-related information.

FIG. 7 shows the contents of information relating to the reagent in the example of the present invention.

As shown in fig. 7, the reagent-related information storage unit 201 stores reagent identification information 301, a corresponding reagent name 302, and reagent position information 303.

The reagent discrimination information 301 is the number information of each reagent bottle 150, and the reagent label on each reagent bottle 150 contains the reagent discrimination information indicating the different number.

The reagent name 302 is a reagent name of the reagent contained in the reagent bottle 150.

The reagent position information 303 includes the cabinet number of the reagent cabinet 1, the layer number of the placing layer 103, and rough position information of the reagent bottle 150 at the rough position of the placing layer 103.

In this embodiment, since the determination units 104 are symmetrically disposed on the left and right sides of each of the placement layers 103 to detect the left and right sides of the placement layer 103, respectively, and the reagent bottles 150 located in the middle of the placement layers 103 can be determined by the two determination units 104 at the same time, the rough position information corresponds to the left, middle, and right sides of the placement layers 103, and the laboratory technician can directly search for the desired biological reagents at the corresponding positions of the placement layers 103 according to the information.

As can be seen from the above figure, as the reagent position information corresponding to the reagent discrimination information P1 and P2, the case number and the layer number are the same, indicating that two reagent bottles 150 containing different reagents are stored in the same reagent storage layer 15 of the same reagent case 1, but one of the two reagent bottles 150 is on the left side of the reagent storage layer and the other is on the right side.

The reagent identification information P2, P3, and P4 correspond to the same reagent name, but the corresponding reagent position information is different, indicating that three reagent bottles containing the same reagent are placed at different positions (the reagent bottles corresponding to P2 and P3 are placed on different placement levels 15 of the same reagent box 1, and the reagent bottles corresponding to P4 are placed in other reagent boxes 1).

The reagent position information corresponding to the reagent discrimination information P5 and P7 is null, and indicates that the reagent bottle corresponding to the reagent discrimination information is in a state of being extracted by the laboratory technician and is not in the reagent tank 1.

In this embodiment, the information may be entered and stored by a manager who logs in the management server 2 while purchasing a new biological reagent using the management terminal, or the information may be stored by introducing existing information into the management server 2. In addition to the above-mentioned information association storage, other necessary related information such as the reagent specification and the use necessity of the biological reagent may be associated with the reagent discrimination information and stored.

In this embodiment, when the experimenter applies for the reagent, the experimenter terminal 3 needs to log in the management server 2 by using the identification information, and the name of the required reagent is input through the human-computer interaction picture of the experimenter terminal 3. Subsequently, the laboratory technician terminal 3 transmits the input required reagent name to the management server 2, and then the management server 2 retrieves consistent reagent information from the reagent-related information according to the received required reagent name, thereby further acquiring the reagent position information and transmitting the reagent position information and the received required reagent name as extraction information to the laboratory technician terminal 3.

Fig. 8 is the content of the extracted information in the embodiment of the present invention.

Taking the input required reagent name SJ2 as an example, as shown in fig. 8, the extraction information 221 includes the reagent name 2211 and the corresponding reagent position information 2212. It can be seen that the reagent position information in the extraction information 221 may be multiple, and reagents representing the needs of the laboratory technician are stored at multiple positions.

Fig. 9 is contents of a request record in the embodiment of the present invention.

Meanwhile, the management server 2 also stores the required reagent name, the extraction information, and the identification information received from the laboratory technician terminal 3 as one request record while transmitting the extraction information. As shown in fig. 9, the management server 2 stores therein a plurality of request records 206, each of which includes a required reagent name 251, all-reagent-position information 252 corresponding to the required reagent name 251, and identification information 253 of a laboratory technician who requested the required reagent name 251. Through each request record, the management server 2 can conveniently judge whether the laboratory technician has the authority to open the reagent cabinet 1 according to the cabinet number in the reagent position information.

In step S2, when the laboratory technician selects one reagent kit 1 and takes in and out the biological reagent, the identification information of the laboratory technician is identified by the identification unit, and the identification information is transmitted to the management server 2 via the reagent kit communication unit 1101 of the reagent kit 1, and the process proceeds to step S3.

In step S3, the management server 2 determines whether the laboratory technician has the authority to open the reagent kit 1 based on the received identification information and the kit number, and transmits a door opening permission signal to the corresponding reagent kit 1 if it is determined that the laboratory technician has the authority to open the reagent kit 1, and then the process proceeds to step S4.

Fig. 10 is the content of the extraction record in the embodiment of the present invention.

When a laboratory technician takes out a certain biological reagent from the reagent cabinet 1, the management server 2 stores the corresponding reagent discrimination information and the identification information received from the reagent cabinet 1 as one extraction record, thereby forming a corresponding extraction record. As shown in fig. 10, the management server 2 stores a plurality of extraction records 207, each including a piece of missing reagent discrimination information 291, corresponding identification information 292, and reagent position information 293, the missing reagent discrimination information 291 being extracted by an experimenter having the identification information 292.

In this embodiment, the management server 2 performs the determination in step S3 on the basis of the request record and the extracted record, and specifically, when retrieving the request record, the management server 2 retrieves the request record according to the received identification information and the cabinet number, and determines whether there is matching identification information and the cabinet number (that is, the cabinet number included in the reagent position information in the extracted information); when retrieving the extracted record, the management server 2 retrieves the extracted record based on the received identification information and determines whether there is matching identification information.

Once any one of the two determinations is determined to exist, the management server 2 sends a door opening permission signal to the reagent cabinet 1, and the main control chip 110 of the reagent cabinet 1 controls the locking part 106 to unlock the cabinet door 105, so that an experimenter can open the cabinet door 105 and perform extraction operation. If neither of the above determinations is determined to be present, no response is made (alternatively, the service-side communication unit 210 may transmit an alert signal to the corresponding reagent kit 1, and the warning unit 109 may issue an alert to alert the laboratory technician).

In step S4, the main control chip 110 controls the locking part 106 to unlock the cabinet door 105, so that the laboratory technician opens the cabinet door 105 and performs an extraction or return operation, that is, searches for and takes out the reagent bottle 150 at the rough position of the placing layer 103 corresponding to the extracted information, or stores the reagent bottle 150 at an arbitrary position of any placing layer 103. Once the experimenter opens the cabinet door 105 so that the sensing part 107 senses that the cabinet door 105 is opened, the process proceeds to step S5; if the sensing part 107 does not sense that the cabinet door 105 is opened within a predetermined time, the main control chip 110 controls the locking part 106 to lock the cabinet door 105 again.

In step S5, the main control chip 110 can control the determination unit 104 to continuously perform the reading operation, and when the sensing unit 107 senses that the cabinet door 105 is closed, the main control chip sends the reagent determination information read by the determination unit 104, the part number of the determination unit 104, the layer number of the corresponding placement layer 103, and the cabinet number of the current reagent cabinet 1 to the management server 2, and then the process proceeds to step S6.

In this embodiment, the reagent discrimination information sent by the reagent cabinet 1 to the management server 2 is the reagent discrimination information of all the reagent bottles 150 in the reagent cabinet 1 immediately before the cabinet door 105 is closed, and at this time, the reagent bottles 150 in the reagent cabinet 1 have been extracted or returned by the experimenter.

In this embodiment, in step S5, when the sensing portion 107 senses that the cabinet door 105 is closed, the main control chip 110 further controls the locking portion 106 to lock the cabinet door 105.

In step S5 of this embodiment, when the sensing part 107 senses that the cabinet door 105 is closed, the main control chip 110 controls the locking part 106 to lock the cabinet door 105.

In step S6, the management server 2 compares the reagent discriminating information received in step S5 with the reagent discriminating information stored in the reagent related information based on the cabinet number and the layer number received in step S5 to determine missing or newly added reagent discriminating information, updates the reagent related information based on these information, and updates the extraction record and the request record, and then proceeds to step S7.

In this embodiment, since two discrimination units 104 are provided for each placement layer 103, when receiving the reagent discrimination information, the management server 2 combines the reagent discrimination information corresponding to the same placement layer 103 and compares the combined information with the reagent discrimination information in the reagent-related information to determine missing or added reagent discrimination information.

Further, when the management server 2 determines the added reagent discrimination information, the corresponding discrimination unit 104 is determined based on the part number corresponding to the added reagent discrimination information, and the rough position information is determined based on the position information of the discrimination unit 104.

In this embodiment, two determination sections 104 are provided for each of the placement levels 103, namely, a left determination section corresponding to the left side of the placement level and a right determination section corresponding to the right side of the placement level, and when the rough position information is set by the rough position determination section, the rough position information is set to the left side (right side) when the rough position information is set by the left determination section (or right determination section), and the rough position information is set to the middle when both determination sections 104 determine that the reagent bottles 150 are present.

After the comparison determination is completed, if the management server 2 compares the missing reagent discrimination information (that is, if the plurality of pieces of reagent discrimination information stored in the reagent-related information include reagent discrimination information that is not included in the received reagent discrimination information and indicate that a reagent bottle is taken out from the placement layer), the management server 2 deletes the corresponding reagent position information in the reagent-related information according to the missing reagent discrimination information; if the management server 2 compares the added reagent discrimination information (that is, the received plurality of pieces of reagent discrimination information include reagent discrimination information that is not included in the reagent discrimination information in the reagent related information and indicate that a new reagent bottle is placed in the placement layer), the management server 2 adds the corresponding cabinet number, layer number, and rough position information as the corresponding reagent position information in the reagent related information according to the added reagent discrimination information.

Further, when the management server 2 determines the missing reagent discrimination information, it updates the extracted record based on the missing reagent discrimination information and the identification information transmitted from the reagent tank 1, and deletes the corresponding request record.

In step S7, the management server 2 checks the extraction record and the request record to determine whether the laboratory technician has extracted or returned the wrong biological reagent, and if the laboratory technician has extracted or returned the wrong biological reagent, the process proceeds to step S8, and if the laboratory technician has not extracted or returned the wrong biological reagent, the process proceeds to the end state.

In this embodiment, after the extraction record is updated in step S6, the management server 2 checks whether or not the reagent discrimination information in the extraction information matches the reagent name corresponding to the reagent discrimination information newly stored in the extraction record based on the identification information, and thereby determines whether or not the laboratory technician has extracted the wrong biological reagent. If the result of the examination is inconsistent, the laboratory staff extracts wrong biological reagents.

Meanwhile, the management server 2 checks the returned biological reagent according to the newly added reagent discrimination information discriminated in step S6 and the cabinet number of the corresponding reagent cabinet 1, thereby determining whether the laboratory technician returned the wrong biological reagent.

In the present embodiment, the reagent-related information stored in the management server 2 further includes a storage temperature range corresponding to the reagent identification information, and the temperature adjustment unit 102 of each reagent kit 1 stores current temperature information corresponding to the current temperature to be adjusted. When the reagent tank communication unit 1101 transmits the reagent discrimination information discriminated by the discrimination unit 104 to the management server 6, the current temperature information is also transmitted to the management server 6 at the same time.

Further, when the management server 2 checks the returned biological reagent, it may determine whether the biological reagent is placed in the wrong reagent cabinet according to whether the received current temperature information is within the storage temperature range corresponding to the missing reagent determination information. If the current temperature information exceeds the storage temperature range, it indicates that the biological reagent is erroneously placed in the reagent storage 1 having an inappropriate storage temperature by the laboratory technician.

In step S8, the management server 3 sends a corresponding warning alert to the reagent kit 1 according to the determination result in step S7, so that the warning unit 109 activates a warning alert to remind the scientific research staff of taking out the wrong reagent, and then enters an end state.

< example two >

In the second embodiment, the same reference numerals are given to the components having the same configurations as those in the first embodiment, and the description thereof will be omitted.

The second embodiment provides a low-temperature reagent cabinet capable of storing biological reagents requiring a low-temperature environment of-30 ℃ to-100 ℃, in which conventional electronic devices cannot work normally, and an RFID card reader and other devices cannot be arranged in the reagent cabinet, so that the design of the reagent cabinet in the second embodiment is different from that of the reagent cabinet in the first embodiment, and the specific differences are as follows:

fig. 11 is a schematic structural diagram of a reagent cabinet according to a second embodiment of the present invention.

As shown in fig. 11, the reagent kit 5 is provided with a kit body 101, a temperature adjusting portion 102, a placing layer 113, an identification portion 108, a warning portion 109 and a main control chip 115, and is different from the first embodiment in that the placing layer 113 of each layer of the reagent kit 5 is provided with a thermal insulation cabinet door 111 and a layer eaves 112 protruding outward to the outside of the reagent kit 1 at the top (i.e. a placing plate of a placing layer on each placing layer 113, and a first placing layer 113a is a top plate of the whole reagent kit 1), each thermal insulation cabinet door 111 is provided with a locking portion 106 and a sensing portion 107, and the placing layer 113 has only three layers.

In addition, two discrimination parts 114 corresponding to each layer of the placing layer 113 are arranged on the layer eaves 112 corresponding to the placing layer 113 and close to the outer side surface of the insulation cabinet door 111, and are used for discriminating reagent labels of all the reagent bottles 150 in the placing layer when an experimenter opens the insulation cabinet door 111 to take out or put in biological reagents, and the reagent labels used in the second embodiment are ultra-low temperature resistant RFID labels, and can normally operate in a low-temperature environment of-100 ℃.

In this embodiment, the discrimination range of the discrimination section 114 can discriminate the entire rack layer 113 to recognize the reagent discrimination positions of all the reagent bottles in each rack layer 113. Meanwhile, in the present embodiment, the discrimination range of each discrimination section 114 is larger than one-half of the width of the placement layer 113 and smaller than the width of the placement layer 113.

Because the determination portion 114 is disposed on the layer eaves 111 (i.e. the area of the outside of the reagent cabinet 5 exposed in the normal temperature), in the second embodiment, the main control chip 115 senses that the insulation cabinet door 111 is opened in the sensing portion 107 and then controls the determination portion 114 to perform determination continuously until the sensing portion 107 senses that the insulation cabinet door 111 is closed by the experimenter, at this time, the main control chip 115 controls the reagent cabinet communication portion 1101 to send all the reagent determination information sensed by each determination portion 104 in the opening and closing process of the insulation cabinet door 111 and the corresponding determination portion number, layer number, cabinet number and identification information of the experimenter to the management server 2.

In the second embodiment, the intelligent management method of the non-immobilized biological reagent based on the reagent cabinet 5 has the same principle as that in the first embodiment, and is not described herein again.

< example three >

In the third embodiment, the same reference numerals are given to the components having the same configurations as those in the first and second embodiments, and the description thereof will be omitted.

The third embodiment provides a biological reagent intelligent management system for uniformly managing a normal temperature reagent cabinet and low temperature reagent cabinets with different temperatures, wherein the normal temperature reagent cabinet and the low temperature reagent cabinet with the temperature of-30 ℃ adopt the same structure as the reagent cabinet 1 in the first embodiment (the temperature is adjusted by the temperature adjusting part 102), and the low temperature reagent cabinet with the temperature of-30 ℃ to-100 ℃ adopts the structure of the reagent cabinet 5 in the second embodiment. At this time, the structure of the system 200 is as shown in fig. 12.

In the third embodiment, the intelligent management method of the non-immobilized biological reagent based on the biological reagent management system 200 has the same principle as that in the first embodiment, and is not described herein again.

Examples effects and effects

According to the intelligent management method for the non-fixed biological reagents provided by the embodiment, the reagent labels are arranged on the reagent bottles in the laboratory, and the distinguishing parts capable of distinguishing the reagent labels are correspondingly arranged on the placing layers of the reagent cabinet, so that after the sensing parts on the reagent cabinet sense that a laboratory worker opens the cabinet door, the distinguishing parts distinguish the change of the reagent distinguishing information corresponding to the reagent bottles on the placing layers, the management server can update the states of the reagent positions and the like according to the reagent distinguishing information, and the accurate management of the reagent bottles aiming at each biological reagent is realized. In addition, because the cabinet number of the reagent cabinet where each reagent bottle is located and the layer number of the corresponding placing layer are recorded, when a laboratory technician needs to extract the biological reagent, the position of the required biological reagent can be easily and quickly found, and therefore the management effect that the position of each reagent bottle can be accurately grasped even if the laboratory technician can conveniently and randomly place the reagent bottle in the reagent cabinet is realized. Further, since the laboratory technician is required to return to any position of the reagent tank after use, the biological reagent is kept at a predetermined low temperature as much as possible, thereby suppressing the deterioration of the original performance.

In addition, in the first embodiment, since the determination unit for identifying the reagent label is disposed between the respective placement layers in the low-temperature environment of-10 ℃ to-30 ℃, once the laboratory technician extracts or returns the biological reagent, the determination unit can identify the reagent determination information of each biological reagent in the reagent cabinet after the cabinet door is closed, and thus the position state of the biological reagent can be counted without the intervention of the laboratory technician, and whether the reagent bottle of the biological reagent is extracted or returned by the laboratory technician can be accurately determined.

Furthermore, in the first embodiment, the management server further has a rough position determination part, and at least two determination parts are correspondingly arranged on each placement layer in the reagent cabinet, so that the layers are placed on each layer, the approximate positions of the reagent bottles can be determined according to the reagent determination information read by the determination parts, and the positions of the reagent bottles are more clearly represented in the reagent position information, so that a laboratory technician can find out the required reagents in time, and meanwhile, the biological reagents are stored in the low-temperature refrigerated reagent cabinet, so that the laboratory technician can more timely find out the required reagents, and can effectively avoid the loss of cold air in the reagent cabinet due to long-time door opening and reduce the time of the laboratory technician in the low-temperature environment.

In addition, in the second embodiment, since the outwardly protruding layer eaves are provided for each of the placement layers and the determination part is provided below each layer eaves in the low-temperature environment of-30 ℃ to-100 ℃, the determination part that is difficult to operate at an excessively low temperature can be provided at a normal temperature, and the identification of the reagent discrimination information of each biological reagent in the placement layer is realized in a short time when the laboratory technician opens the cabinet door. By the method, the intelligent management method for the non-fixed biological reagent realizes the effect of effectively and accurately managing all reagent bottles in the reagent cabinet at extremely low temperature.

In addition, in the third embodiment, when the normal temperature reagent cabinet and the low temperature reagent cabinet are mixed and set in the laboratory, when the laboratory returns the reagent, the management server can determine whether the biological reagent returned by the laboratory is placed in the wrong reagent cabinet, and the warning part of the reagent cabinet warns and reminds, so that the biological reagent is prevented from being damaged and lost due to the fact that the storage temperature of the biological reagent is not consistent with the actual temperature in the reagent cabinet, and the laboratory returns the wrong biological reagent in the reagent cabinet due to errors and the like (for example, the reagent with small difference due to close color) is prevented.

Further, because when judging that the wrong reagent is returned, because the discrimination portion of reagent cabinet sets up on the layer eaves in the second embodiment, consequently can discern the reagent discrimination information that the laboratory technician put into more rapidly, at this moment, thereby the management server also can accomplish more rapidly and judge and make warning portion remind the laboratory technician to put into wrong reagent more timely to avoid the laboratory technician to the influence that the biological reagent caused when taking place to return the mistake better. And to the reagent cabinet in the embodiment one, because the temperature is higher relatively, can not lead to the fact the effect of freezing more fast to biological reagent, consequently remind the laboratory technician relatively more slowly can not cause biological reagent's loss.

The above-described embodiments are merely illustrative of specific embodiments of the present invention, and the present invention is not limited to the description of the above-described embodiments.

For example, in the above embodiment, the reagents corresponding to the reagent discrimination information included in the extracted information are all extracted by the experimenter, however, in another aspect of the present invention, the experimenter may acquire only the reagents corresponding to part of the extracted information. At this time, the extracted information display screen further has a reagent selecting portion. Specifically, when the same reagent name in the relevant information of the reagent has a plurality of corresponding reagent discrimination information, the extracted information includes a plurality of reagent discrimination information, and after the extracted information is sent to the laboratory technician terminal, the extracted information display screen displays each reagent name and corresponding reagent position information in the extracted information on the reagent selecting part, so that the laboratory technician selects one or more reagents to view in detail, and at the moment, the extracted information display screen displays the reagent position information corresponding to the selection of the laboratory technician. Therefore, the intelligent management method of the non-immobilized biological reagent can allow an experimenter to selectively extract the reagent.

For example, in the first embodiment, two symmetrically arranged determination portions are provided for each placement layer, and the rough position information is the left side and the right side corresponding to the positions of the determination portions, respectively. In other aspects of the invention, each placing layer can be correspondingly provided with more RFID readers and is uniformly arranged in the placing layer, and the number of corresponding rough positions is further increased, so that the rough position information is more accurate, and the position of the reagent bottle on the placing layer can be more accurately judged.

In addition, in the second embodiment, two determination portions are provided on the layer eaves corresponding to each layer of the placement layer, and the range of the determination portions can cover the entire placement layer. In other schemes of the invention, in order to facilitate the discrimination part to discriminate the whole placement layer, a form of a directional RFID reader can be adopted, the directional RFID reader is installed slightly obliquely outward, i.e. the reading direction of the directional RFID reader faces inward, and meanwhile, an inward inclined slope part can be arranged on the layer brim at the position of the RFID reader, so that electromagnetic waves of the RFID reader enter the placement layer to read the sample label on the biological sample in the placement layer.

Further, when in actual use, more discrimination parts can be correspondingly arranged under each layer of eave according to actual requirements, so that the accuracy of discrimination of rough position information is improved.

For another example, in the reagent kit according to the second embodiment, the reagent discrimination information recognized by the judgment section is the reagent discrimination information of all the biological reagents in the entire rack, and the management server judges the missing or added reagent discrimination information by the comparison judgment section. However, in another embodiment of the present invention, the determination unit provided on the layer eaves may directly identify the reagent extracted or returned by the laboratory technician, that is, the reagent passes through the layer eaves when the laboratory technician takes out (or returns) the reagent from the placement layer, and in this case, the determination unit may identify the corresponding reagent determination information, and further transmit the reagent determination information to the management server, and the management server may determine whether the reagent is extracted or placed in the original reagent-related information based on whether the reagent determination information is present in the original reagent-related information.

In addition, in the scheme of the invention, the normal-temperature reagent cabinet and the low-temperature reagent cabinet mixed in the third embodiment can adopt the structure of the reagent cabinet in the second embodiment. At this moment, all reagent cabinets can realize going on more fast that biological reagent puts into wrong warning to avoid the experimenter to the influence that biological reagent caused when taking place to return the mistake better.

For example, in the embodiment, the identification information of the laboratory technician and the request and extraction record of the laboratory technician for the reagent are stored through the request record and the extraction record, and the identity information judging part further judges whether the laboratory technician has the authority to open the cabinet door of the reagent cabinet. In another embodiment, the management server may store identification information of all laboratory technicians, and in this case, the management server does not set the request record and the extraction record. When the reagent cabinet sends the identification information to the management server for judgment, the management server directly searches the identification information according to the received identification information and judges whether the identification information is consistent with the identification information, if the identification information is consistent with the identification information, the management server sends door opening permission information to the reagent cabinet, and if the identification information is not consistent with the identification information, the management server does not respond.

For example, in the above embodiment, the warning portion is a warning lamp provided on the front surface of the cabinet door. In another aspect of the present invention, the warning unit may be a sound-light alarm, a display, or another device having a presentation effect, and may be provided at a position on the reagent tank where the warning unit is easily viewed.

Claims (7)

1. An intelligent management method for non-fixed biological reagents is characterized in that a system comprising a low-temperature reagent cabinet for storing biological reagents needing low-temperature environment freezing storage, a laboratory terminal and a management server is adopted to realize the management of the biological reagents with reagent labels in a laboratory, and comprises the following steps:

correspondingly storing the reagent name of each biological reagent, the corresponding reagent identification information and corresponding reagent position information comprising the cabinet number of the low-temperature reagent cabinet where the reagent bottle is located and the layer number of the placed layer in the management server as reagent related information;

enabling the experimenter to input the name of the biological reagent to be extracted as a required reagent name by adopting the experimenter terminal and sending the required reagent name to the management server;

the management server retrieves the reagent related information according to the sent required reagent name, acquires corresponding reagent position information, and sends the acquired reagent position information and the required reagent name serving as extraction information to the laboratory technician terminal;

the laboratory technician terminal displays the received extraction information to allow the laboratory technician to go to the corresponding low-temperature reagent cabinet according to the extraction information;

adopting an identity recognition part of the low-temperature reagent cabinet to recognize the identification information of the experimenter and sending the identification information to the management server for judgment;

judging whether the laboratory technician has the authority to open the cabinet door or not at least based on the identification information by adopting the management server, and sending a door opening permission signal to the low-temperature reagent cabinet once the laboratory technician has the authority to open the door;

the control module of the low-temperature reagent cabinet unlocks a cabinet door according to the door opening permission signal so as to enable the experimenter to open the cabinet door for extraction or storage operation;

adopting a judging part to continuously judge reagent judging information contained in all reagent labels in the reagent cabinet and adopting a sensing part to sense whether the cabinet door is closed, and sending the read reagent judging information, the current cabinet number of the low-temperature reagent cabinet and the layer number of the corresponding placing layer to the management server once sensing that the laboratory technician closes the cabinet door;

the management server judges the access condition of the biological reagent and updates the reagent-related information at least according to the transmitted reagent judgment information, the cabinet number and the layer number.

2. The intelligent management method of non-immobilized bioreagent according to claim 1, wherein:

the determination part continuously determines the reagent determination information contained in all the reagent labels in the reagent cabinet based on that at least two determination parts with different part numbers are correspondingly arranged on each placing layer, and the specific process is as follows:

sending the read reagent identification information, the current cabinet number of the low-temperature reagent cabinet, the layer number of the corresponding placement layer and the part number corresponding to the identification part to the management server;

the management server also judges rough position information corresponding to the reagent discrimination information according to the received reagent discrimination information and the layer number;

and when updating the reagent position information in the reagent related information, the management server also updates the reagent related information according to the rough position information.

3. The intelligent management method for non-immobilized bioreagent according to claim 2, wherein:

and when the laboratory technician terminal displays the received extraction information, the rough position information is also displayed, so that the laboratory technician searches the required biological reagent at the corresponding rough position in the corresponding placing layer according to the rough position information.

4. The intelligent management method of non-immobilized bioreagent according to claim 1, wherein:

wherein, when the management server judges the access condition of the biological reagent, the method specifically comprises the following steps:

comparing the received reagent discrimination information with the reagent discrimination information in the reagent-related information to discriminate a missing reagent discrimination information corresponding to the taken-out biological reagent or an added reagent discrimination information corresponding to the returned biological reagent;

and updating the reagent-related information according to the missing reagent discrimination information or the added reagent discrimination information.

5. The intelligent non-immobilized bioreagent management method according to claim 4, wherein:

when the management server judges the added reagent judgment information, whether the corresponding biological reagent is placed in the wrong reagent cabinet is judged according to the added reagent judgment information and the corresponding cabinet number.

6. The intelligent management method of non-immobilized bioreagent according to claim 5, wherein:

wherein each low-temperature reagent cabinet is provided with different low-temperature environments which are from-10 ℃ to-100 ℃,

the reagent-related information further stores a storage temperature range corresponding to a required storage temperature of the biological reagent,

the management server determining whether the biological reagent is placed in a wrong reagent cabinet is performed based on the storage temperature range and current temperature information corresponding to the low-temperature environment of the reagent cabinet, specifically comprising the following steps:

judging whether the current temperature information corresponding to the cabinet number is in the storage temperature range corresponding to the added reagent judgment information;

if the current temperature information is judged to be in the storage temperature range, judging that the biological reagent is not placed in a wrong reagent cabinet;

and if the current temperature information is not within the storage temperature range, the biological reagent is placed in the wrong reagent cabinet, and the management server sends a warning signal to the corresponding low-temperature reagent cabinet.

7. The intelligent management method of non-immobilized bioreagent according to claim 5, wherein:

wherein the management server updates the reagent-related information and stores reagent discrimination information corresponding to the biological reagent determined to be extracted and identification information received from the low-temperature reagent tank as one extraction record,

the management server judges whether the biological reagent is put into the wrong reagent cabinet or not based on the extraction record, and the method specifically comprises the following steps:

determining whether the biological reagent is loaded into a wrong reagent tank based on whether the received tank number matches the reagent position information corresponding to the reagent discrimination information in the extraction record,

if the cabinet number is judged to be consistent with the reagent position information, judging that the biological reagent is not put into a wrong reagent cabinet;

and if the cabinet number is judged to be inconsistent with the reagent position information, judging that the biological reagent is placed in the wrong reagent cabinet, and sending a warning signal to the corresponding low-temperature reagent cabinet by the management server.

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