CN113180157A - Traditional Chinese medicine composition for improving reproductive performance of sows and active ingredients - Google Patents
Traditional Chinese medicine composition for improving reproductive performance of sows and active ingredients Download PDFInfo
- Publication number
- CN113180157A CN113180157A CN202110414607.4A CN202110414607A CN113180157A CN 113180157 A CN113180157 A CN 113180157A CN 202110414607 A CN202110414607 A CN 202110414607A CN 113180157 A CN113180157 A CN 113180157A
- Authority
- CN
- China
- Prior art keywords
- sows
- sow
- chinese medicine
- traditional chinese
- improving
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
- A23K20/126—Lactones
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Birds (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a traditional Chinese medicine composition for improving the reproductive performance of sows and an effective component. The traditional Chinese medicine composition for improving the reproductive performance of the sows consists of angelica sinensis, ligusticum wallichii, radix paeoniae alba and prepared rehmannia root. The invention adopts LC-MS combined technology to identify chemical components of the compound traditional Chinese medicine composition entering milk, and the result determines that 5 components of the blood components, namely sedanolide, oxypaeoniflorin, catechin, albiflorin and ligustilide, are effective components playing a role in the traditional Chinese medicine composition, play a key role in improving the reproductive performance of sows, and provide a certain guiding basis for the action mechanism research and the next scientific research of the compound traditional Chinese medicine composition.
Description
Technical Field
The invention relates to a pharmaceutical composition for improving the reproductive performance of sows, in particular to a traditional Chinese medicine composition for improving the reproductive performance of sows and an effective component, belonging to the field of medicines for improving the reproductive performance of sows.
Background
In the production practice of sows, diseases such as postpartum agalactia, hypogalactia, constipation, oestrus failure, metritis, vaginitis and the like generally exist, and traditional Chinese medicine considers that qi and blood are the most basic substances forming the bodies of human beings and livestock and maintaining the life activities of the human beings and the livestock, if the qi and the blood are not harmonious, all diseases are generated, and in the production of livestock and poultry, the health of the livestock and poultry is seriously affected, so that the dysfunction of viscera, the reduction of the production performance of animals and even the occurrence of diseases are caused. At present, sows in China generally have qi and blood deficiency, Chinese veterinary medicines are green medicines generally regarded as having development prospects in recent years, and research and development of application of classical qi and blood tonifying formulas in the pig raising industry has important significance for solving the problems of the sows at present.
Disclosure of Invention
One of the purposes of the invention is to provide a traditional Chinese medicine composition for effectively improving the reproductive performance of sows;
the second purpose of the invention is to provide an effective component for effectively improving the reproductive performance of sows;
in order to achieve the purpose, the technical scheme adopted by the invention comprises the following steps:
the invention firstly provides a traditional Chinese medicine composition for improving the reproductive performance of sows, which consists of angelica sinensis, ligusticum wallichii, radix paeoniae alba and prepared rehmannia root.
In order to achieve better technical effects, the weight parts of the components are preferably as follows: 9-15 parts of angelica, 9-20 parts of ligusticum wallichii, 9-15 parts of radix paeoniae alba and 9-20 parts of prepared rehmannia root.
Wherein the improvement of the reproductive performance of the sow comprises any one or more indexes from the following (1) to (6):
(1) improving the healthy litter size, the newborn litter weight of piglets, the number of weaned heads of piglets in 21 days, the number of weaned litter in 21 days and the daily lactation capacity;
(2) shortening the birth process and farrowing interval of the sow;
(3) increasing the levels of estrogen and prolactin in the postpartum serum of a lactating sow;
(4) increasing hemoglobin and NO levels in lactating sows;
(5) the content of milk protein and milk fat in the colostrum of the lactating sow is improved;
(6) improving the contents of insulin growth factor, epidermal growth factor and immunoglobulin G in the colostrum of the sow.
The invention adopts LC-MS coupling technology to identify the chemical components of the Chinese medicinal composition entering milk, and the result determines that 5 components of the Chinese medicinal composition entering blood, namely sedanolide, oxypaeoniflorin, catechin, albiflorin and ligustilide, are effective components of the compound Chinese medicinal composition, thereby playing a role in improving the reproductive performance of sows.
Therefore, the invention further provides an effective component for effectively improving the reproductive performance of sows, and the effective component is selected from any one or more than one of sedanolide, oxypaeoniflorin, catechin, albiflorin or ligustilide according to any proportion; preferably, the effective components for improving the reproductive performance of sows consist of sedanolide, oxypaeoniflorin, catechin, albiflorin and ligustilide.
The invention further provides a method for improving the reproductive performance of sows by applying the traditional Chinese medicine composition, which comprises the following steps: pulverizing all the components of the compound traditional Chinese medicine composition, uniformly mixing and adding the pulverized components into a basal ration of a sow; for reference, the addition amount of the compound traditional Chinese medicine composition added to the basal diet of the sow can be 0.05-2 wt%, and preferably 0.2 wt%;
or mixing one or more of sedanolide, oxypaeoniflorin, catechin, albiflorin or ligustilide at any ratio to obtain composition, and adding into basal diet of sow; for reference, the effective ingredient may be added to the basal diet of sows in an amount of 0.01-1 wt%, preferably 0.05 wt%.
The invention screens the difference between the milk of the administration group and the milk of the blank group by adopting a metabonomics method based on LC-MS, thereby researching the influence of the traditional Chinese medicine composition on the lactating sow, and the result shows that 5 effective components enter the milk to play a role, thereby providing a certain guiding basis for the action mechanism research of the compound traditional Chinese medicine and the next scientific research.
Detailed description of the invention
First, the compound traditional Chinese medicine composition is used for investigating the reproductive performance of lactating sows
The compound traditional Chinese medicine composition is added into the basic daily ration by 0.2 percent, and the test period is 35 days. During the period, production indexes are recorded, and milk is collected at 0, 7 and 21 days after delivery, and the components in the milk, colostrum immunoglobulin, growth factor, etc. are measured. Blood collection was performed on day 0, 7, and 21 after delivery, and estrogen, progesterone, and prolactin were measured.
The test results show that the compound traditional Chinese medicine composition can improve the number of healthy piglets, the weight of newborn litter of piglets, the number of weaned heads of piglets in 21 days, the number of weaned litter in 21 days and the daily lactation power, and has significant difference (p is less than 0.05) compared with a control group. The compound traditional Chinese medicine can shorten the birth process and farrowing interval of the sow, and has significant difference (p is less than 0.05) compared with a control group; the compound traditional Chinese medicine composition is added into the feed of the sow, so that the levels of estrogen and prolactin in serum of the lactating sow on the 1 st day and the 7 th day after delivery (p is less than 0.05) can be improved, and the difference is obvious compared with that of a control group (p is less than 0.05). The feed added with the compound traditional Chinese medicine can improve the hemoglobin and NO levels of the lactating sows, and has obvious difference (p is less than 0.05) compared with a control group; the compound traditional Chinese medicine composition is added into the feed, so that the content of milk protein and milk fat in the colostrum of the lactating sow can be improved, and the difference is obvious compared with that of a control group (p is less than 0.05); the compound traditional Chinese medicine composition can improve the contents of insulin growth factors, epidermal growth factors and immunoglobulin G in the colostrum of the sows, and has obvious difference (p is less than 0.05) compared with a control group.
Secondly, determining the effective components of the compound traditional Chinese medicine in the milk of the sow
The LC-MS combined technology is adopted to identify chemical components of the compound traditional Chinese medicine in the milk, and 5 components of the blood components, namely sedanolide, paeoniflorin, catechin, albiflorin and ligustilide, are determined to be effective components of the compound traditional Chinese medicine composition for improving the reproductive performance of sows, so that the key effect of improving the reproductive performance of sows is achieved.
Drawings
Figure 1 results of the effect of the Chinese medicinal composition on sow hormones.
Figure 2 results of the effect of the traditional Chinese medicine composition on the hemoglobin and nitric oxide of sows.
Figure 3 the effect of the Chinese medicinal composition of the invention on the milk composition of lactating sows.
Figure 4 the effect of the Chinese medicinal composition of the present invention on growth factors and immunoglobulins in the milk of sows.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It is to be understood that the described embodiments are exemplary only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be within the scope of the invention.
Test example 1 influence of the Chinese medicinal composition on reproductive performance of lactating sows and screening of effective ingredients
1 materials and methods
1.1 materials
1.1.1 test Chinese medicine
The compound traditional Chinese medicine composition consists of angelica, ligusticum wallichii, radix paeoniae alba and prepared rehmannia root, and the dosage of each component is as follows: 12g of angelica, 16g of ligusticum wallichii, 12g of radix paeoniae alba and 16g of prepared rehmannia root.
1.1.2 test animals
The test is carried out in Liaoning Weijia pig farm, 40 healthy pigs of 3 births are selected totally, and are randomly divided into two groups, wherein 20 pigs are selected in each group, and the test period is from 110 days of gestation to weaning.
1.1.3 reagents and instruments
The kit is a product of Beijing Huaying biotechnology research institute, and the Nexera UHPLC LC-30A ultra-high performance liquid phase is produced by Shimadzu corporation, the Triple TOF 5600 high resolution mass spectrum analyzer and the Milko-Scan 134A/B infrared milk component analyzer are produced by the Denmark Foss corporation.
1.2 test methods
1.2.1 study of reproductive Performance of lactating sows with the Chinese medicinal composition
Approximately 40 pigs were randomized into 2 groups of 20 pigs each. Feeding basal diet to control group; the test group added 0.2% of the tested traditional Chinese medicine into the basic daily ration, and the test period was 35 days. During the period, production indexes are recorded, and milk is collected at 0, 7 and 21 days after delivery, and the components in the milk, colostrum immunoglobulin, growth factor, etc. are measured. Blood collection was performed on day 0, 7, and 21 after delivery, and estrogen, progesterone, and prolactin were measured.
1.2.2 identification of effective ingredients in Compound traditional Chinese medicine milk
100mg of the test drug was added to 500. mu.L of the extraction solution containing 1. mu.g/mL of the internal standard, and the mixture was vortexed for 1 minute and then sonicated in 10 ℃ water for 1 h. After standing at-40 ℃ for 1 hour, the sample was centrifuged at 12000rpm for 15 minutes at 4 ℃. Finally, the supernatant was obtained and placed in a new 2mL tube for LC-MS/MS analysis. mu.L of a sample of pig milk was added to 40. mu.L of hydrochloric acid (2mol/L), and the mixture was vortexed for 1 minute, and then incubated at 4 ℃ for 15 minutes. The vortexing and incubation periods were repeated 4 times. 1.6mL of acetonitrile was added, the mixture was vortexed for 5 minutes, and the sample was centrifuged at 12000rpm for 5 minutes at 4 ℃. 1600 μ L of the supernatant was transferred to a new tube and dried under nitrogen. The dried sample was reconstituted in 200. mu.L of 80% methanol containing 1. mu.g/mL of internal standard for 5 minutes. Then centrifuged at 12000rpm for 5 minutes at 4 ℃ and 80. mu.L of the supernatant was transferred to a fresh glass vial for LC/MS analysis.
2 results of the test
2.1 Effect of tested Chinese herbs on the productivity of lactating sows
The tested traditional Chinese medicine can improve the healthy litter size, the newborn litter weight of piglets, the number of weaned heads of the piglets in 21 days, the number of weaned litter in 21 days and the daily lactation power, and has significant difference (p is less than 0.05) compared with a control group. The compound traditional Chinese medicine can shorten the birth process and the farrowing interval of the sows, and has significant difference (p is less than 0.05) compared with a control group.
TABLE 1 Effect of tested Chinese herbs on lactating sows Productivity
Note: compared with the control group, the compound of the formula,*p＜0.05，**p＜0.01
2.2 Effect of tested Chinese herbs on hormones in lactating sows
The effect of the compound traditional Chinese medicine on the concentration of estrogen, progestogen and prolactin in the serum of the lactating sow on the 1 st day, the 7 th day and the 21 st day after delivery is shown in figure 1. As can be seen from the figure 1, the compound traditional Chinese medicine added in the feed can improve the levels of estrogen and prolactin in the serum of the lactating sow on the 1 st day and the 7 th day after delivery (p is less than 0.05), has a remarkable difference (p is less than 0.05) compared with a control group, and has no remarkable difference on the influence of the progestogen compared with the control group.
2.3 Effect of tested Chinese herbs on lactating mother hemoglobin and nitric oxide
The effect of the compound traditional Chinese medicine on the hemoglobin and Nitric Oxide (NO) of the postpartum lactating sow is shown in figure 3. As can be seen from fig. 3, the feed added with the compound traditional Chinese medicine can improve the hemoglobin and NO levels of the lactating sows, and has obvious difference (p is less than 0.05) compared with the control group
2.4 Effect of tested Chinese herbs on lactating sow milk composition
The effect of the compound traditional Chinese medicine on lactose, milk protein, milk fat and dry matter in milk of lactating sows at 1, 7 and 21 days after delivery is shown in figure 3. As can be seen from fig. 3, the feed added with the compound traditional Chinese medicine can improve the content of milk protein and milk fat in colostrum of the lactating sow, and has a significant difference (p is less than 0.05) compared with a control group.
2.5 Effect of tested Chinese herbs on growth factors and immunoglobulins in lactating sow milk
The effect of the compound Chinese medicine on growth factors and immunoglobulin in sow milk is shown in figure 4. As can be seen from FIG. 4, the compound Chinese medicine can increase the contents of insulin growth factor, epidermal growth factor and immunoglobulin G in colostrum, and has a significant difference (p < 0.05) compared with the control group.
2.6 determination of effective Components of Chinese medicine to be tested in sow milk
The LC-MS combined technology is adopted to identify the chemical components of the compound traditional Chinese medicine in the milk, and 5 blood components are determined (as shown in the table 2). Therefore, the sedanolide, the oxypaeoniflorin, the catechin, the albiflorin and the ligustilide are effective components of the compound traditional Chinese medicine and play a key role in improving the reproductive performance of sows.
TABLE 2 chemical composition of tested Chinese medicine in milk samples
Claims (10)
1. A Chinese medicinal composition for improving reproductive performance of sows is characterized by comprising Chinese angelica, Szechuan lovage rhizome, white paeony root and prepared rehmannia root.
2. The traditional Chinese medicine composition according to claim 1, which is characterized by comprising the following components in parts by weight: 9-15 parts of angelica, 9-20 parts of ligusticum wallichii, 9-15 parts of radix paeoniae alba and 9-20 parts of prepared rehmannia root.
3. An effective component for improving the reproductive performance of sows is characterized in that the effective component is any one or more than one of sedanolide, oxypaeoniflorin, catechin, albiflorin or ligustilide in any proportion.
4. The active ingredient of claim 3, wherein the active ingredient consists of sedanolide, oxypaeoniflorin, catechin, albiflorin and ligustilide.
5. The compound traditional Chinese medicine composition as claimed in claim 1, wherein the sow reproductive performance improvement is selected from any one or more of the following (1) to (6):
(1) improving the healthy litter size, the newborn litter weight of piglets, the number of weaned heads of piglets in 21 days, the number of weaned litter in 21 days and the daily lactation capacity;
(2) shortening the birth process and farrowing interval of the sow;
(3) increasing the levels of estrogen and prolactin in the postpartum serum of a lactating sow;
(4) increasing hemoglobin and NO levels in lactating sows;
(5) the content of milk protein and milk fat in the colostrum of the lactating sow is improved;
(6) improving the contents of insulin growth factor, epidermal growth factor and immunoglobulin G in the colostrum of the sow.
6. The active ingredient as claimed in claim 3 or 4, wherein the improvement of reproductive performance of sows is selected from any one or more of the following (1) to (6):
(1) improving the healthy litter size, the newborn litter weight of piglets, the number of weaned heads of piglets in 21 days, the number of weaned litter in 21 days and the daily lactation capacity;
(2) shortening the birth process and farrowing interval of the sow;
(3) increasing the levels of estrogen and prolactin in the postpartum serum of a lactating sow;
(4) increasing hemoglobin and NO levels in lactating sows;
(5) the content of milk protein and milk fat in the colostrum of the lactating sow is improved;
(6) improving the contents of insulin growth factor, epidermal growth factor and immunoglobulin G in the colostrum of the sow.
7. The use of the Chinese medicinal composition of claim 1 or 2 for improving reproductive performance of sows, comprising: the components in the compound traditional Chinese medicine composition are crushed and uniformly mixed to be added into the basal ration of the sow.
8. The use as claimed in claim 7, wherein the compound Chinese medicinal composition is added to the basal ration of the sow in an amount of 0.05-2 wt%, preferably 0.2 wt%.
9. The use of the active principle of claim 3 or 4 for improving the reproductive performance of sows, comprising: the components in the traditional Chinese medicine composition are crushed and uniformly mixed to be added into the basal ration of the sow.
10. Use according to claim 9, characterized in that the active ingredient is added to the basal ration of the sow in an amount of 0.01-1%, preferably 0.05% wt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110414607.4A CN113180157A (en) | 2021-04-16 | 2021-04-16 | Traditional Chinese medicine composition for improving reproductive performance of sows and active ingredients |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110414607.4A CN113180157A (en) | 2021-04-16 | 2021-04-16 | Traditional Chinese medicine composition for improving reproductive performance of sows and active ingredients |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113180157A true CN113180157A (en) | 2021-07-30 |
Family
ID=76977356
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110414607.4A Pending CN113180157A (en) | 2021-04-16 | 2021-04-16 | Traditional Chinese medicine composition for improving reproductive performance of sows and active ingredients |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113180157A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104367739A (en) * | 2014-10-21 | 2015-02-25 | 郑州后羿制药有限公司 | Galactagogue for sows and preparation method of galactagogue |
| CN104824454A (en) * | 2015-05-18 | 2015-08-12 | 新希望六和饲料股份有限公司 | Sow formula feed and preparation method thereof |
| CN106071247A (en) * | 2016-07-16 | 2016-11-09 | 陈珠 | A kind of in-pig expected date of confinement feedstuff |
| CN106620044A (en) * | 2016-12-07 | 2017-05-10 | 吉林省畜牧兽医科学研究院 | Traditional Chinese medicine composition for preventing miscarriage of pregnant sows and preparation method thereof |
-
2021
- 2021-04-16 CN CN202110414607.4A patent/CN113180157A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104367739A (en) * | 2014-10-21 | 2015-02-25 | 郑州后羿制药有限公司 | Galactagogue for sows and preparation method of galactagogue |
| CN104824454A (en) * | 2015-05-18 | 2015-08-12 | 新希望六和饲料股份有限公司 | Sow formula feed and preparation method thereof |
| CN106071247A (en) * | 2016-07-16 | 2016-11-09 | 陈珠 | A kind of in-pig expected date of confinement feedstuff |
| CN106620044A (en) * | 2016-12-07 | 2017-05-10 | 吉林省畜牧兽医科学研究院 | Traditional Chinese medicine composition for preventing miscarriage of pregnant sows and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 褚秀玲等: "中兽医气血理论及其在母猪生产中的应用", 《中兽医医药杂志》, vol. 35, no. 4, pages 574 - 85 * |
| 贾魁荣: "古方四物汤的复方活性物质", 《中国临床医生》, vol. 36, no. 8, pages 21 - 22 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Penagos Tabares et al. | Pharmacological overview of galactogogues | |
| Al-Qarawi et al. | Gastrointestinal transit in mice treated with various extracts of date (Phoenix dactylifera L.) | |
| CN103283959A (en) | Chinese herbal ultra-fine powder feed additive and its application in culture | |
| CN107281110B (en) | Tannic acid protein powder capable of being administrated by drinking water and preparation method thereof | |
| Mallick et al. | Influence of feeding Tinospora cordifolia peripartum on lactation parameters in crossbred cows | |
| CN103520111B (en) | A kind of florfenicol dry suspension and its production and use | |
| CN102688323A (en) | Chinese veterinary drug granule for improving immunologic function of livestock and preparation method thereof | |
| CN113180157A (en) | Traditional Chinese medicine composition for improving reproductive performance of sows and active ingredients | |
| CN101618087B (en) | Health-care food with function of improving climacteric | |
| CN103892048B (en) | Piglet is calmed the nerves to increase and exempts from growth-promoting feedstuff additive, preparation method and application thereof | |
| CN113262246A (en) | Traditional Chinese medicine composition for improving livestock immunity and effective components | |
| CN116098938A (en) | Treatment method for improving milk cow reproduction rate and milk yield by adopting Chinese herbal medicine preparation and application | |
| Mogheiseh et al. | Comparing the Vitex-castus extract and cabergoline effects on estrous induction in female dogs | |
| Niu et al. | Impact of fermented Broussonetia papyrifera on laying performance, egg quality, lipid metabolism, and follicular development of laying hens | |
| Mohammad et al. | Milk thistle seed extract favorably affects lactation and development of mammary gland in female rats | |
| Sinha et al. | Study of pattern of morbidity in children under 5 years and effect of Swarnaprashan on morbidity status | |
| CN1814048A (en) | Chinese medicine liquid capsule of Folium callicarpae Nudiflorae, preparing method and quality control method | |
| CN104873940B (en) | A kind of filling liver kidney tocolysis powder and the application in preparing prevention equine abortion drug | |
| CN111298099A (en) | Iron-supplementing composition and preparation method and application thereof | |
| JP4578760B2 (en) | Climacteric disorder treatment composition, sex hormone elevation composition | |
| Melampy et al. | Estrogen excretion by cows after oral administration of diethylstilbestrol | |
| CN116999488B (en) | Traditional Chinese medicine composition, preparation method thereof and application thereof in preparation of sow lactation products | |
| CN113262252B (en) | Novel medicine composition for preventing vinasse poisoning of cattle and sheep, preparation method and use method | |
| Turini et al. | Effect of a Phytogenic Feed Additive in Preventing Calves' Diarrhea | |
| CN103405389B (en) | Veterinary menbutone powder and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220325 Address after: 150081 3-1-1801, mengke Hanshe, No. 6, Jianfu Road, Nangang District, Harbin City, Heilongjiang Province Applicant after: Li Yanhua Address before: 150030 Heilongjiang Province, Harbin city Xiangfang District Gongbin Road No. 201 Applicant before: Harbin pinhe Biotechnology Co.,Ltd. |