CN113166809A - 一种dna甲基化检测的方法、试剂盒、装置和应用 - Google Patents
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Abstract
本申请提供了一种DNA甲基化检测的方法，包括长片段DNA处理步骤，将长片段DNA打断；常规文库构建步骤，用stLFR技术建库；双链保护步骤，取回收的stLFR文库，DNA变性，用退火或延伸方法将分子标签或接头序列转化为双链；重亚硫酸盐转化步骤，加入双链保护剂和重亚硫酸盐，进行脱氨基；甲基化测序文库构建步骤，对脱氨基产物进行常规PCR或环化后滚环扩增，得到甲基化测序文库；甲基化测序步骤，对甲基化测序文库测序，根据测序结果分析基因的甲基化信息；本申请还提供了相应试剂盒、装置和应用。本申请方法利用stLFR技术，获得长片段甲基化信息；通过双链保护策略，可事先加入分子标签序列建库，可有效追踪分子来源。
Description
PCT国内申请，说明书已公开。
Claims (23)
- PCT国内申请，权利要求书已公开。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811642291 | 2018-12-29 | ||
| CN2018116422919 | 2018-12-29 | ||
| PCT/CN2019/127537 WO2020135347A1 (zh) | 2018-12-29 | 2019-12-23 | 一种dna甲基化检测的方法、试剂盒、装置和应用 |
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| CN113166809A true CN113166809A (zh) | 2021-07-23 |
| CN113166809B CN113166809B (zh) | 2023-12-26 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023082251A1 (zh) * | 2021-11-15 | 2023-05-19 | 深圳华大智造科技股份有限公司 | 一种基于标签序列和链置换的全基因组甲基建库测序方法 |
| CN116343919A (zh) * | 2023-04-11 | 2023-06-27 | 天津大学四川创新研究院 | 一种全基因组图谱绘制测序方法 |
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- 2019-12-23 WO PCT/CN2019/127537 patent/WO2020135347A1/zh active Application Filing
- 2019-12-23 CN CN201980076223.7A patent/CN113166809B/zh active Active
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