CN116343919A - 一种全基因组图谱绘制测序方法 - Google Patents
一种全基因组图谱绘制测序方法 Download PDFInfo
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- CN116343919A CN116343919A CN202310381659.5A CN202310381659A CN116343919A CN 116343919 A CN116343919 A CN 116343919A CN 202310381659 A CN202310381659 A CN 202310381659A CN 116343919 A CN116343919 A CN 116343919A
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- 238000012163 sequencing technique Methods 0.000 title claims abstract description 96
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 30
- 241000208340 Araliaceae Species 0.000 claims abstract description 5
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 5
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 5
- 235000008434 ginseng Nutrition 0.000 claims abstract description 5
- 238000013507 mapping Methods 0.000 claims abstract description 5
- 239000000523 sample Substances 0.000 claims description 23
- 230000000295 complement effect Effects 0.000 claims description 12
- 210000000349 chromosome Anatomy 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 238000005259 measurement Methods 0.000 claims description 6
- 241000894007 species Species 0.000 claims description 5
- 238000012937 correction Methods 0.000 claims description 3
- 238000002864 sequence alignment Methods 0.000 claims description 2
- 238000007481 next generation sequencing Methods 0.000 description 21
- 230000008901 benefit Effects 0.000 description 8
- 230000002068 genetic effect Effects 0.000 description 4
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 3
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- 238000012350 deep sequencing Methods 0.000 description 3
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- 238000007480 sanger sequencing Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/30—Detection of binding sites or motifs
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B25/10—Gene or protein expression profiling; Expression-ratio estimation or normalisation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
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- Bioinformatics & Computational Biology (AREA)
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- Medical Informatics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Theoretical Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103336916A (zh) * | 2013-07-05 | 2013-10-02 | 中国科学院数学与系统科学研究院 | 一种测序序列映射方法及系统 |
WO2015149719A1 (zh) * | 2014-04-04 | 2015-10-08 | 深圳华大基因科技服务有限公司 | 杂合基因组处理方法 |
KR20180083706A (ko) * | 2017-01-13 | 2018-07-23 | 주식회사 클리노믹스 | 공통서열을 포함한 참조표준 게놈지도 구축 장치 및 방법 |
CN113166809A (zh) * | 2018-12-29 | 2021-07-23 | 深圳华大生命科学研究院 | 一种dna甲基化检测的方法、试剂盒、装置和应用 |
WO2021232388A1 (zh) * | 2020-05-22 | 2021-11-25 | 深圳华大智造科技有限公司 | 确定胚胎细胞染色体中预定位点碱基类型的方法及其应用 |
WO2022261874A1 (zh) * | 2021-06-16 | 2022-12-22 | 深圳华大生命科学研究院 | 一种通过单链滚环扩增获得双链序列的方法 |
CN115810395A (zh) * | 2022-12-05 | 2023-03-17 | 武汉贝纳科技有限公司 | 一种基于高通量测序动植物基因组t2t组装方法 |
CN115938480A (zh) * | 2021-09-23 | 2023-04-07 | 武汉华大基因技术服务有限公司 | 长读长测序对基因组组装结果纠错方法优化装置和系统 |
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2023
- 2023-04-11 CN CN202310381659.5A patent/CN116343919B/zh active Active
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103336916A (zh) * | 2013-07-05 | 2013-10-02 | 中国科学院数学与系统科学研究院 | 一种测序序列映射方法及系统 |
WO2015149719A1 (zh) * | 2014-04-04 | 2015-10-08 | 深圳华大基因科技服务有限公司 | 杂合基因组处理方法 |
KR20180083706A (ko) * | 2017-01-13 | 2018-07-23 | 주식회사 클리노믹스 | 공통서열을 포함한 참조표준 게놈지도 구축 장치 및 방법 |
CN113166809A (zh) * | 2018-12-29 | 2021-07-23 | 深圳华大生命科学研究院 | 一种dna甲基化检测的方法、试剂盒、装置和应用 |
WO2021232388A1 (zh) * | 2020-05-22 | 2021-11-25 | 深圳华大智造科技有限公司 | 确定胚胎细胞染色体中预定位点碱基类型的方法及其应用 |
WO2022261874A1 (zh) * | 2021-06-16 | 2022-12-22 | 深圳华大生命科学研究院 | 一种通过单链滚环扩增获得双链序列的方法 |
CN115938480A (zh) * | 2021-09-23 | 2023-04-07 | 武汉华大基因技术服务有限公司 | 长读长测序对基因组组装结果纠错方法优化装置和系统 |
CN115810395A (zh) * | 2022-12-05 | 2023-03-17 | 武汉贝纳科技有限公司 | 一种基于高通量测序动植物基因组t2t组装方法 |
Non-Patent Citations (3)
Title |
---|
刘海琳等: "全基因组测序技术研究及其在木本植物中的应用", 南京林业大学学报(自然科学版), no. 05 * |
孙丹等: "长读长测序技术在宏基因组学研究中的应用", 生命科学, no. 08 * |
武汉百奥维凡生物: "常用转录组组装软件集合", pages 13 - 18, Retrieved from the Internet <URL:https://zhuanlan.zhihu.com/p/431665296> * |
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CN116343919B (zh) | 2023-12-08 |
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