CN113143840A - Facial mask composition containing marine-source skin DNA repair cell anti-aging compound and preparation method thereof - Google Patents
Facial mask composition containing marine-source skin DNA repair cell anti-aging compound and preparation method thereof Download PDFInfo
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- CN113143840A CN113143840A CN202110312378.5A CN202110312378A CN113143840A CN 113143840 A CN113143840 A CN 113143840A CN 202110312378 A CN202110312378 A CN 202110312378A CN 113143840 A CN113143840 A CN 113143840A
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- dna repair
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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Abstract
The invention discloses a facial mask composition containing a marine-derived skin DNA repair cell anti-aging compound and a preparation method thereof. The anti-aging mask composition comprises: 1.0-30.0 percent of DNA repair component, 5.0-40.0 percent of emollient, 0.5-30.0 percent of emulsifier, 0.5-20.0 percent of filler, 0.1-10.0 percent of thickener, 0.3-5.0 percent of preservative, 0.05-0.5 percent of EDTA-2NA, 0.01-1.0 percent of daily essence and the balance of deionized water. The DNA repair composition comprises: algae extract, sea oak extract, cerevisiae Fermentum extract, and lactobacillus fermentation lysate. The marine-derived skin DNA repair cell anti-aging cosmetic composition has anti-aging, pulling and tightening effects on facial skin, has a regulating effect on the expression of metallothionein, NQO1, Ki67 and MMP1, and has an enhancing effect on a dermis structure through targeting type I collagen and hyaluronic acid. In addition, clinical studies prove that the compound can enhance the antioxidant capacity of the skin.
Description
Technical Field
The invention relates to a cosmetic composition, belongs to the technical field of daily chemicals, and particularly relates to a facial mask composition containing a marine-derived skin DNA repair cell anti-aging compound, and a preparation method and application thereof.
Background
The accumulation of damage during aging is associated with a failure of homeostasis, which is dynamic. Sursh i.s. rattan considers that the dynamic space is a survival/buffering capacity, determining survival, quality and lifetime. It can be considered our vitality. More precisely, it refers to the ability of the living system to react to internal and external stresses through an effective stress response, and to counteract any disturbances threatening its survival by neutralization and/or by adaptation, by injury control and management, by continuous remodeling and adaptation over the life span. A very promising strategy against skin aging has recently been to apply an exciting concept, which is well known in aging studies, based on the principle of stimulating maintenance and repair pathways to balance stress, which is a better preparation of skin cells against more intense stimuli. The term excitatory describes a biphasic dose response to environmental factors (physical, chemical or biological) characterized by a low dose stimulating or beneficial effect and a high dose inhibiting or toxic effect on cells or organisms.
During aging, the progressive reduction of dynamic space in the body leads to an increase in the fragile zone and the development of age-related diseases, which also act at the cellular level. Skin aging is a complex biological process, influenced by a combination of intrinsic and extrinsic factors that affect the dynamic space in the body. Failure of this gap will lead to changes in the appearance of the skin (e.g., wrinkles, pigmentation irregularities, appearance of neoplastic skin lesions) and to molecular damage and impaired functional capacity of the various levels of tissue of each of the key skin layers (epidermis, dermis and hypodermis).
Therefore, it is important to be able to regulate the stress response by regulating the balance between damage and repair through stress response and repair pathways, especially in connection with exposure to ultraviolet radiation and various environmental pollutants (e.g., volatile organic compounds, particulates, fumes, ozone, heavy metals). To achieve stability through dynamic interactions of maintenance and repair, several explanations are being tried to understand this mechanism.
The dynamic capacity of a biological system is a biphasic dose response affected by stress, called physiological excitation. Lower levels of stress result in a dynamic enhancement in one stimulation zone (H). Chronic and severe stress can lead to a reduction and disruption of in vivo kinetics, leading to functional impairment, disease and ultimately death.
Depending on the severity and duration of the stress encountered, cells respond to various stresses through a number of self-maintenance and repair pathways to counteract destructive effects in the cell. These pathways may include induction of the Nrf 2-based SR pathway, induction of the NFkB pathway, DNA damage response, induction of hsp70, or autophagy and sirtuin-dependent SR pathways.
In fact, these stress pathways are very specific and not all stresses. The specificity of the response depends mainly on the nature of the damage caused by the stressor and the kind of downstream effectors involved.
The idea of applying the beneficial effects proposed by the concept of excitement, but how to propose a new marine complex, which is resistant to intrinsic and extrinsic skin aging through various adaptive abilities against various stresses, in particular oxidative stress and metal-induced stress, is a technical problem that those skilled in the art are urgently required to solve.
Disclosure of Invention
The invention aims to disclose a facial mask composition containing marine-derived skin DNA repair cell anti-aging compound, which comprises two extracts extracted from brown seaweed dogwood and soaked fucoidin and a selenium-supplementing saccharomyces cerevisiae extract, wherein the extracts are complementary in antioxidant performance.
Another object of the present invention is to provide a method for preparing the above facial mask composition containing marine-derived skin DNA repair cell anti-aging complex, wherein the prepared marine complex provides a natural innovative approach for resisting skin cell stress response, and defensive cell stress response is coordinated through overexpression of unique markers (such as metallothionein MT-1 and MT-2) to maximize cell adaptation and survival. By stimulating fibroblast proliferation, increasing synthesis of collagen and hyaluronic acid, enhancing skin structure, and promoting maintenance of extracellular matrix. Significant cosmetic benefits in improving facial skin aesthetics were demonstrated by two groups of clinical studies (versus placebo) and consumer use evaluations.
The present invention is realized by the following technical means.
The facial mask composition containing the marine-derived skin DNA repair cell anti-aging compound comprises the following components in parts by weight:
1.0-30.0% of a DNA repair composition comprising: 0.1-20% of algae extract, 0.1-20% of fucus extract, 0.1-30% of saccharomyces cerevisiae extract and 0.1-30% of lactobacillus fermentation lysate;
5.0-40.0% of an emollient;
0.5-30.0% of emulsifier;
0.5-20.0% of a filler;
0.1-10.0% of a thickening agent;
0.3-5.0% of preservative;
0.05-0.5% EDTA-2 NA;
0.01-1.0% of daily essence and the balance of deionized water.
Further, the cosmetic composition is composed of the following components by weight:
DNA repair function 10.0-30.0%, comprising: 2.0-20% of sargassum elongatum (HIMANTHALIA ELONGATA) extract, 2.5-20% of Fucus VESICULOSUS (Fucus VESICULOSUS) extract, 3.5-30% of SACCHAROMYCES CEREVISIAE (Saccharomyces CEREVISIAE) extract and 2.0-30% of lactobacillus fermentation lysate;
further, the DNA repair promoting component comprises the following raw material components in percentage by weight of the total weight of the composition:
1.0-10% of extract of Zostera marina (HIMANTHALIA ELONGATA); 1.0-10% of Fucus VESICULOSUS (Fucus VESICULOSUS) extract; 1.0-20% of brewer's yeast (Saccharomyces CEREVISIAE) extract; 1.0-20% of lactobacillus fermentation lysate.
Further, the DNA repair promoting component comprises the following raw material components in percentage by weight of the total weight of the composition:
5.0-15% of the extract of the algae Lasiocladia elongata (HIMANTHALIA ELONGATA); 5.0 to 15 percent of FUCUS VESICULOSUS (FUCUS VESICULOSUS) extract; 5.0-20% of beer yeast (Saccharomyces CEREVISIAE) extract; 5.0-18% of lactobacillus fermentation lysate.
Further, the emollient is selected from at least one of caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, glycerin, butylene glycol, hexylene glycol, pentylene glycol, polydimethylsiloxane, dimethiconol, PEG-11 methyl ether dimethicone, phytosterol/octyldodecyl lauroyl glutamate, hydrogenated polyisobutene, rapeseed oil, polydecene, soybean oil, olive oil, isododecane, isohexadecane, stearyl alcohol, and behenyl alcohol. The content is selected from the range of 5-30%, preferably the range of 8-15%.
The emulsifier is selected from at least one of PEG-7 esters of olive oil, glyceryl PEG-8 caprylate/caprate, sorbitan olive oleate, polysorbate-20, laureth-23, PEG-40 stearate, steareth-21, palmityl alcohol and PEG-75 stearate and mixtures of glyceryl stearate and steareth-20, polysorbate-80, soya lecithin PC60, mixtures of glyceryl stearate and PEG-75 stearate, poloxamer 407, PEG-30 dipolyhydroxystearate, and ceteareth-25. The content is selected from the range of 0.5-15%, preferably 0.5-10%.
The filler is at least one selected from titanium dioxide, mica, boron nitride, polymethylsilsesquioxane, diatomite, kaolin, cellulose acetate, aluminum starch octylsuccinate, talcum powder, barium sulfate and volcanic ash. The content is in the range of 0.5-15%, preferably in the range of 2% -12%.
The thickening agent is at least one selected from hydroxyethyl cellulose, xanthan gum, carbomer, acrylic acid (ester)/copolymer, polymer EMT-10, polyacrylate cross-linked polymer-6, sodium acrylate/sodium acryloyldimethyl taurate copolymer, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, acryloyldimethyl taurate/VP copolymer, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, polyethylene and gum arabic. The content is selected from the range of 0.3-8%, preferably 0.5-5%.
The preservative is at least one selected from phenoxyethanol, ethylhexyl glycerol, pentanediol, hexanediol, aroyl ketone, sodium benzoate, triclosan and chlorphenesin.
In addition, the invention also discloses a preparation method of the anti-aging mask composition with the DNA repairing component, and the preparation method comprises the following process steps:
1) wetting and completely dispersing the thickening agent in water to obtain a mixed solution;
2) adding DNA repair promoting ingredients, an emollient, a filler, a preservative and EDTA-2NA into the mixed solution obtained in the step 1), heating to 65 ℃, and homogenizing at 3000r/min for 3 minutes to completely disperse the mixed solution;
3) adding an emulsifier and a daily essence into the step 2), homogenizing at 3000r/min for 3 minutes to completely emulsify the mixture, and heating to 65 ℃ to obtain the cosmetic composition with DNA repair.
Specifically, the cosmetic of the DNA repair composition of the present invention has a pH in the range of 4 to 10, preferably a pH in the range of 5 to 8, and most preferably a pH in the range of 5 to 7.
Specifically, the cosmetic composition of the DNA repair composition of the present invention may be in the form of a solvent dispersion or suspension, an emulsified particle, or a polymer-coated form.
Specifically, the cosmetic of the DNA repair composition of the present invention is a skin care product, in particular, a lotion, essence, cream, eye cream, body lotion, gel, sunscreen lotion.
By means of the technical scheme, the invention has the following advantages and beneficial technical effects:
1) the invention discloses a cosmetic composition containing DNA repair components, wherein the marine-source skin DNA repair cell anti-aging cosmetic components have anti-aging, pulling and tightening effects on facial skin, have regulating effects on the expression of metallothionein, NQO1, Ki67 and MMP1, and have enhancing effects on a dermis structure by targeting I type collagen and hyaluronic acid; the complex can activate the anti-stress gene regulatory network, which means that the appropriate adaptive response is initiated and the skin is protected from aging and environmental stress.
2) In addition, clinical studies prove that the compound can enhance the antioxidant capacity of skin;
3) finally, subjective evaluation on volunteers shows that the marine-derived skin DNA repair cell anti-aging cosmetic ingredients are beneficial to making facial skin smoother, stronger and more shiny; overall, the complex provides an innovative and adaptive defense method, leading to a tighter and better protection of the facial skin.
Drawings
FIG. 1 is a graph of increased cell proliferation;
FIG. 2 is a graph of increased synthesis of type I collagen-philic proteins;
FIG. 3 is a graph of increased synthesis of hyaluronic acid;
FIG. 4a is a graph of clinical skin antioxidant effect as determined by FRAP measurements;
FIG. 4b is a graph of clinical anti-lipid oxidation of skin as measured by MDA;
FIG. 5 is a graph of a subject evaluation performed on volunteers who applied 4% composite gel for 28 days.
Detailed Description
The invention discloses a cosmetic composition for improving a DNA repair function and a preparation method thereof. The cosmetic composition comprises, based on the total weight of the composition: 0.1-30% of algae (HIMANTHALIA ELONGATA) extract, 0.1-30% of Fucus VESICULOSUS (Fucus VESICULOSUS) extract, 0.1-40% of Saccharomyces CEREVISIAE (Saccharomyces CEREVISIAE) extract, and 0.1-30% of lactobacillus fermentation lysate; and a cosmetically acceptable carrier; through the preferable combination and efficacy test, the most preferable composition proportion is found to realize the DNA repair function of the skin.
The raw material components are as follows: the extract of the algae Lasiocladia elongata, the extract of the Fucus vesiculosus and the extract of the Saccharomyces cerevisiae can activate an anti-stress gene regulation network, promote a homeostatic pathway (homeostatic pathways), and contribute to enhancing the cell detoxification function and protecting cells from cascade reactions affected by harmful environments. Can maximize the adaptive capacity of cells and make the skin better resist the invasion and the injury.
The structural integrity and function of the dermal layer is dependent on the extracellular matrix (ECM) composed of various molecules secreted by different fibroblasts. As skin begins to age, changes occur in fibroblasts as well as in the major constituent molecules of the extracellular matrix.
Can act on fibroblast and partial extracellular matrix components, and particularly has obvious effect on collagen I and hyaluronic acid.
The skin is protected from environmental stress stressors by a unique adaptive capacity against different types of stress, in particular against oxidative stress and heavy metal induced stress. Can activate anti-stress gene regulation network by coordinating defensive cell stress response and promoting cascade reaction of oxidative stress (HSPA1A, NQO1) related to harmful environment and 'homeostatic pathway' related to cell detoxification (metallothionein MT-1, MT-1), thereby maximizing adaptability and viability of cells.
The algae belonging to the family Lasiocladaceae (Himanthaliaceae) is the only one belonging to the order Fucales (Fucales) and the only one belonging to the genus Laminaria (Himanthalia). The thallus is divided into two morphological stages, wherein the first morphological stage is vegetative thallus like a button; and an elongate frond.
The algae sargassum elongatum is a specific algae in europe, and can be found in norway along the coast of the north atlantic to the north of spain and portugal all the time; but not in the pacific region. The algae is rich in sodium, potassium and magnesium minerals capable of removing free radicals. "pasta in the sea (seaspaghetti)", "thongweed (thongweed)", and "button weed (button weed)". It is a common food material in france and ireland.
Fucus vesiculosus is a kind of brown algae growing on rocks attached to the shore. It grows on the coast of British Brantany, France where abundant marine organisms are gathered, and can be harvested during the ebb tide. The algae body is bifurcated and is a flat horn-shaped leaf, and the algae body is provided with an air bag and is easy to distinguish.
In China, Fucus vesiculosus has been used for the prevention and treatment of iodine deficiency goiter since the beginning of the 16 th century. Beginning in 1818, it was recorded into Pharmacopoea Galica (French classics); in 1988, they were listed on a positive list for human consumption by the French National Council for Health.
In addition to anti-cellulite activity, Fucus vesiculosus also inhibits heat-induced collagen glycation, and has anti-collagenase and anti-elastase activity. Can increase skin thickness and elasticity, and is effective in resisting aging.
The brewers' yeast extract is a selenium enriched yeast (Se-Ye) with interesting chemical components and excellent antioxidant properties. The Saccharomyces cerevisiae extract is obtained by adding selenium element during culture process and fermenting.
During the growth of beer yeast, selenium is converted into biological selenium mainly through the combination of 2 kinds of selenium-containing amino acids (selenic acid and seleniomethionin) and protein and polysaccharide in beer yeast, so that the toxic side reaction and gastrointestinal stimulation of chemical selenium (such as sodium selenite) to human body are eliminated, selenium can be absorbed and utilized by human body more efficiently and safely, and the main selenoprotein in cells is enzyme with antioxidant property, such as glutathione peroxidase and thioreductase. Thus, it has excellent antioxidant properties. The selenium-enriched yeast has special value for animal, human and crop nutrition, even in the cosmetic industry.
Since 2000, multiple licensed applications of selenium enriched yeast for food use have been approved by the U.S. Food and Drug Administration (FDA), European Food Safety Agency (EFSA).
The invention can have the effects of resisting aging, lifting and tightening facial skin, regulating the expression of metallothionein, NQO1, Ki67 and MMP1, and enhancing the dermal structure by targeting type I collagen and hyaluronic acid.
Thus, the complex can activate the anti-stress gene regulatory network, which means that the appropriate adaptive response is initiated and the skin is protected from aging and environmental stress.
In addition, clinical studies prove that the compound can enhance the antioxidant capacity of the skin. Finally, subjective evaluation on volunteers shows that the marine-derived skin DNA repair cell anti-aging cosmetic ingredients are beneficial to making facial skin smoother, stronger and more shiny. Overall, the complex provides an innovative and adaptive defense method, leading to a tighter and better protection of the facial skin.
The invention discloses an anti-aging facial mask composition containing marine-source skin DNA repair cells and a preparation method and application thereof. The mask composition is prepared from the following raw materials:
1.0-30.0 percent of DNA repair component, 5.0-40.0 percent of emollient, 0.5-30.0 percent of emulsifier, 0.5-20.0 percent of filler, 0.1-10.0 percent of thickener, 0.3-5.0 percent of preservative, 0.05-0.5 percent of EDTA-2NA, 0.01-1.0 percent of daily essence and the balance of deionized water.
The facial mask composition contains marine-source skin DNA repair cell anti-aging cosmetic components, and comprises the following components: extract of Lawsonia inermis (HIMANTHALIA ELONGATA), extract of Fucus VESICULOSUS (Fucus VESICULOSUS), extract of Saccharomyces CEREVISIAE (Saccharomyces CEREVISIAE), and lactobacillus fermentation lysate.
The present invention is illustrated by the following examples, which are provided for the purpose of further illustration only and are not intended to limit the scope of the present invention. Other insubstantial modifications and adaptations of the present invention can be made without departing from the scope of the present invention. Unless otherwise specified, the following percentages (%) are mass percentages.
Synergistic test example (example 1 and comparative examples 1 to 5)
Example 1
A facial mask composition with DNA repair function is prepared into an anti-aging skin-care firming facial mask, and the contents of raw material components and raw material components of a control example are shown in the following table 1.
Table 1 raw material component ratios (unit: mass%) of example 1 and comparative examples 1 to 5
The preparation process flow of the embodiment of the invention is as follows:
1) sequentially adding the phase A raw materials into a main tank, and stirring and dispersing completely;
2) slowly adding each grease raw material of the phase B, homogenizing at 3000r/min for 3 min, and confirming complete dispersion;
3) sequentially adding the phases C, and stirring until the phases C are completely dispersed;
4) sequentially adding the D-phase raw materials, emulsifying, and homogenizing at 3000r/min for 3 min;
5) adjusting the pH to be about 6.0;
6) filtering with 100 meshes and discharging.
TABLE 2 anti-aging results example 2 and comparative examples 1-5
| Sample (I) | Example 2 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 |
| ROS content | 72.01％ | 93.11％ | 90.22％ | 77.22％ | 80.82％ | 88.24％ |
As can be seen from Table 2, the order of the aging resistance is: example 2> comparative example 3> comparative example 4> comparative example 5> comparative example 2> comparative example 1.
Anti-aging capability: example 2> comparative example 3+ comparative example 5. Comparative example 3 contains only 2 major anti-aging ingredients, mainly to protect against external damage. Comparative example 5 contained only 2 major anti-aging ingredients, primarily for endogenous oxidation. When the anti-aging agent is used alone for external damage or endogenous oxidation, the anti-aging effect is not outstanding. The anti-aging effect is much better when both are present (i.e., example 2) than when both are combined.
Anti-aging capability: example 2> comparative example 2+ comparative example 4. Comparative example 2 contains only 2 anti-aging ingredients, mainly against external damage, and the content of the corresponding anti-aging ingredients is higher than that in example 2; comparative example 4 contained only 2 major anti-aging ingredients, mainly for endogenous oxidation, and the corresponding anti-aging ingredient content was high for example 2. Theoretically, the sum of the aging resistances of comparative example 2 and comparative example 4 is higher than that of comparative example 2, and it can be actually said that the aging resistance of comparative example 2 is higher than that of comparative example 2 and comparative example 4. This is an unforeseeable effect.
The effect of single target on resisting external damage or single target on endogenous oxidation is not superior to the compound synergistic effect of the internal and external aspects.
In conclusion, the comprehensive anti-aging cosmetic composition achieves comprehensive and efficient anti-aging effect through the synergistic effect among the components.
Example 2
A facial mask composition with DNA repair function is prepared into an anti-aging repairing skin-brightening facial mask, and the contents of the raw material components and the raw material components of a control example are shown in the following table 3.
Table 3 raw material component ratio (unit: mass%) of example 2 and comparative example 2
The preparation process flow of the anti-aging repairing skin-brightening mask provided by the embodiment of the invention is as follows:
1) sequentially adding the phase A raw materials into a main tank, and stirring and dispersing completely;
2) slowly adding each B phase powder, homogenizing at 3000r/min for 3 min, and determining complete dispersion;
3) sequentially adding C-phase grease, and stirring until the grease is completely dispersed;
4) sequentially adding the D-phase raw materials, emulsifying, and homogenizing at 3000r/min for 3 min;
5) adding the phase E in sequence, and adjusting the pH to be about 6.0;
6) filtering with 100 meshes and discharging.
The following is a content of the effect verification test example section.
1. Enhancing cellular defense against various insults through overexpression of metallothionein
As shown in table 4, the marine-derived skin DNA repair cell anti-aging cosmetic composition induces overexpression of MT1G, MT1H, and MT2A genes, which indicates that the novel marine-derived skin DNA repair cell anti-aging cosmetic composition can enhance the cellular defense against various kinds of invasion (heavy metals, active oxygen), thereby preventing exogenous skin aging and enhancing immune function during skin aging. Metallothionein is a very potent protein that can withstand a variety of stresses. Furthermore, their overexpression in aging cells and cells exposed to sunlight may be an interesting target for combating intrinsic and extrinsic skin aging.
TABLE 4 Marine Complex modulation Gene expression Gene name deflection P values for unique markers associated with adaptive cellular stress response
NQO1 can strengthen the antioxidation defense, improve the skin toxin expelling system to the maximum extent, and regulate the oxidation-reduction state of cells
The NQO1 gene encodes a chemoprotective enzyme associated with the cell detoxification system, increasing folding by 1.95%. The marine-derived skin DNA repair cell anti-aging cosmetic ingredient is folded to induce the over-expression of the gene, so that the anti-oxidation defense is increased, the skin detoxification system is improved to the maximum extent, and the regulation of the cell redox state is facilitated.
3. Improvement of dermal structures by promoting cell proliferation
The antigen Ki-67 is a nuclear protein, which is involved in cell proliferation and may be essential for cell proliferation. In addition, it is involved in ribosomal RNA transcription (8, 9). Transcriptomics results showed that Ki-67 increased to 1.64 (table 5), and cell proliferation assays showed that marine-derived skin DNA repair cells anti-aging cosmetic ingredients enhanced cell proliferation (fig. 1). FIG. 1 shows the increase in cell proliferation (normal human fibroblasts cultured in medium for 48 hours) with the nutrient test products dimethyl sulfoxide (10%), fetal bovine serum (10%) and various doses of the complex (0.125% -0.25% -0.5%).
TABLE 5 transcriptomics results
1. Improvement of dermal structure by collagen synthesis
Type i collagen is the major structural protein of the dermis. Collagen fibrils are formed from procollagen fibrils, subsequently assembled into a network, and then stabilized by crosslinking (10). It imparts mechanical strength to the tissue, thereby participating in maintaining the firmness of the tissue. The synthesis result of the type I procollagen confirms that the marine-derived skin DNA repair cell anti-aging cosmetic ingredients highly and remarkably stimulate the synthesis of the type I collagen in a dose-dependent manner. The new anti-aging cosmetic for the marine skin DNA repair cells comprises 0.25%, 0.5% and 1% of the components respectively, and the synthesis of I-type collagen is increased by 24%, 23% and 47% respectively.
FIG. 2 shows the increase in type I procollagen synthesis (type I procollagen production assessed by ELISA assay after 48 hours of treatment with various doses of marine derived skin DNA repair cell anti-aging cosmetic ingredients (0.25%, 0.5% and 1%). Standard vitamin C (50 μ g/ml). Results were calculated as type I procollagen (PIP) concentration (ng/mL).
2. Improvement of dermal structure by hyaluronic acid synthesis
Hyaluronic Acid (HA) is a linear non-sulfated glycosaminoglycan, consisting of repeating units of D-glucuronic acid and N-acetyl-D-glucosamine. Due to its unique water retention capacity, it is called a key molecule for skin moisturization. It provides a framework for a variety of functions, such as hydration, joint lubrication, space filling capacity, and cell migration. The new marine-source skin DNA repair cell anti-aging cosmetic ingredient induces the hyaluronic acid to be synthesized and remarkably increased. Therefore, it can stimulate skin density while regulating skin moisture. FIG. 3 shows the increase in hyaluronic acid synthesis (evaluation of hyaluronic acid synthesis by ELISA test after 48 hours of treatment with different doses of marine-derived skin DNA repair cell anti-aging cosmetic ingredients (0.25%, 0.5% and 1%). TGF-. beta.1 (20ng/mL) was used as a standard.
3. Improvement of dermal structures by down-regulation of MMP-1 gene
Matrix Metalloproteinases (MMPs) are a major class of enzymes that are capable of cleaving one or several extracellular matrix (ECM) components as well as non-matrix proteins (13). In human skin, MMP-1 is a major protease that initiates cleavage of native fibrillar collagen, primarily type I and type III collagen. MMP-1 cleaves collagen fibrils at a site within its central triple helix. As shown in Table 5, the novel marine-derived skin DNA repair cell anti-aging cosmetic ingredient down-regulated MMP-1 expression. Therefore, the novel marine-derived skin DNA repair cell anti-aging cosmetic ingredient can prevent collagen in extracellular matrix from degrading.
4. Clinical research proves that the novel marine-source skin DNA repair cell anti-aging cosmetic ingredient has an antioxidant effect
The objective of this study was to evaluate the photoprotective ability of new marine-derived skin DNA repair cell anti-aging cosmetic ingredients to maintain the total antioxidant capacity of the skin stratum corneum and to protect the skin lipid components from induced oxidative stress, followed by uv irradiation. UVA in sunlight has 10 times more resistance to lipid peroxidation than UVB, which destroys cell membranes through lipid peroxidation.
Fig. 4a shows the determination of clinical skin antioxidant effect by FRAP measurement (evaluation of antioxidant effect measured 30 days after treatment with a base gel containing 4% of new marine-derived skin DNA repair cells anti-aging cosmetic ingredients). Vitamin E is used as a standard.
As shown in fig. 4a, the novel marine-derived skin DNA repair cell anti-aging cosmetic composition induced a statistically significant improvement in skin antioxidant capacity over T0, compared to placebo gel and vitamin E cream.
The search for the role of the novel marine complexes in reducing lipid oxidation by ultraviolet irradiation was performed using Malondialdehyde (MDA). The results show a statistically significant reduction in basal lipid peroxidation damage induced by the novel marine-derived skin DNA repair cells anti-aging cosmetic ingredient compared to placebo gel and vitamin E cream (fig. 4 b). FIG. 4b shows the clinical anti-lipid-oxidation of skin as measured by malondialdehyde assay (anti-lipid-oxidation after 30 days of treatment with a base gel containing 4% of the new marine-derived skin DNA repair cell anti-aging cosmetic ingredient). Vitamin E is used as a standard.
5. Clinical research proves that the novel marine-source skin DNA repair cell anti-aging cosmetic ingredient has the effect of enhancing muscle and tightening
And evaluating the lifting effect through morphological measurement image analysis. These photographs were taken with a digital camera. The lifting effect was evaluated by measuring the distance between the eyes and the underside of the face. A decrease in this parameter is an indication of the boosting effect. Table 6 lists the mean values of the volunteer ptosis changes, with changes compared to placebo gel (+ 0.04%) being statistically insignificant compared to baseline. Compared to baseline, the change (-1.09%) was statistically significant relative to the gel containing 4% of the new marine-derived skin DNA repair cell anti-aging cosmetic ingredient (table 6 a).
In this study, skin elasticity was calculated as the ratio between residual deformation and the maximum elongation of the skin (table 6b) or Ua/Uf. This ratio is referred to in the literature as the total skin elasticity (table 6c), indicating the ability of the skin to return to its original recovery state following a stress event.
TABLE 6a average value of the volunteer droop variation
TABLE 6b mean values of the changes in the elasticity of the skin of the volunteers
TABLE 6c mean values of the changes in the total elasticity of the skin of the volunteers
1. Subjective evaluation
In order to discuss the effect of the novel marine complex on improving the facial skin state, subjective evaluation is carried out, and the result shows that: 86% of volunteers considered their skin to be smoother, 81% considered their skin to be healthier, and 76% felt their skin to be more radiant.
The novel marine-derived skin DNA repair cell anti-aging cosmetic component provides a dual skin aging prevention method. Its effectiveness depends on two main aspects: various pressures are resisted through unique adaptive capacity, so that the environmental pressure is protected,
especially oxidative stress and metal-induced stress. It maximizes cell adaptation and survival by coordinating defensive cellular stress responses and stimulating a series of "homeostatic pathways" associated with oxidative stress to activate the anti-stress gene regulatory network (NQO1) and cellular detoxification (metallothionein MT-1-MT-2).
Improving the maintenance of dermis. The marine-derived skin DNA repair cell anti-aging cosmetic component increases cell viability (stimulates fibroblast proliferation) and a comfortable molecular network (stimulates synthesis of type I collagen and hyaluronic acid) promotes a matrix structure. These cosmetic effects have been demonstrated by two groups of clinical studies and consumer use validation.
The first group of clinical studies involved substantial skin protection of marine derived skin DNA repair cells anti-aging cosmetic ingredients, which was associated with skin antioxidant capacity (FRAP assay) and effective reduction of skin lipid peroxidation (MDA assay), after 28 days of twice daily application of gels containing 4% active ingredient (tape stripping and placebo gels in 20 female caucasian volunteers).
The second group of clinical studies involved the boosting and compacting effects of marine-derived skin DNA repair cell anti-aging cosmetic ingredients on 21 subjects to evaluate 4% active gel, 21 subjects evaluated placebo gel, twice daily for 28 days. Figure 5 shows the subjective evaluation of subjects using a gel containing 4% complex for 28 days.
The marine complex is able to redesign the facial contours by reshaping sagging areas of the skin, enhancing the firmness and elasticity of the skin. As a result, the skin condition of the whole face is improved and the contour is improved.
In this work, we investigated the effect of marine-derived skin DNA repair cells anti-aging cosmetic ingredients on facial skin. In summary, the complex has a modulating effect on the expression of metallothionein, NQO1, Ki67 and MMP1 and an enhancing effect on the dermal structure by targeting type I collagen and hyaluronic acid.
Thus, the complex can activate the anti-stress gene regulatory network, which means that the appropriate adaptive response is initiated and the skin is protected from aging and environmental stress. In addition, clinical studies prove that the compound can enhance the antioxidant capacity of the skin. Finally, subjective evaluation on volunteers shows that the marine-derived skin DNA repair cell anti-aging cosmetic ingredients are beneficial to making facial skin smoother, stronger and more shiny. Overall, the complex provides an innovative and adaptive defense method, leading to a tighter and better protection of the facial skin.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (9)
1. An anti-aging mask composition containing marine-derived skin DNA repair cell complexes is characterized in that: the mask composition consists of the following components in percentage by weight of the total composition:
1.0-30.0% of a DNA repair composition comprising: 0.1-20% of algae extract, 0.1-20% of fucus extract, 0.1-30% of saccharomyces cerevisiae extract and 0.1-30% of lactobacillus fermentation lysate;
5.0-40.0% of an emollient;
0.5-30.0% of emulsifier;
0.5-20.0% of a filler;
0.1-10.0% of a thickening agent;
0.3-5.0% of preservative;
0.05-0.5% EDTA-2 NA;
0.01-1.0% of daily essence and the balance of deionized water.
2. The anti-aging mask composition of claim 1, wherein: the mask composition consists of the following components in percentage by weight based on the total weight of the composition:
10.0-30.0% of a DNA repair composition comprising: 2.0-20% of a sea algae elongation extract, 2.5-20% of an Fucus vesiculosus extract, 3.5-30% of a beer yeast extract and 2.0-30% of a lactobacillus fermentation lysate;
3. the anti-aging mask composition of claim 1, wherein: the DNA repair component comprises the following raw material components by weight:
1.0-10% of the extract of the algae Laribis elongata; 1.0-10% of Fucus vesiculosus extract; 1.0-20% of beer yeast extract; 1.0-20% of lactobacillus fermentation lysate.
4. The anti-aging mask composition of claim 1, wherein: the DNA repair component comprises the following raw material components by weight:
5.0-15% of the extract of the algae Lasiocladia elongata; 5.0 to 15 percent of fucus extract; 5.0-20% of beer yeast extract; 5.0-18% of lactobacillus fermentation lysate.
5. The anti-aging mask composition of claim 1, wherein:
the emollient is at least one of caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, glycerol, butanediol, hexanediol, pentanediol, polydimethylsiloxane, dimethiconol, PEG-11 methyl ether dimethicone, phytosterol/octyldodecyl lauroyl glutamate, hydrogenated polyisobutene, rapeseed oil, polydecene, soybean oil, olive oil, isododecane, isohexadecane, stearyl alcohol, and behenyl alcohol;
the emulsifier is selected from at least one of olive oil PEG-7 esters, PEG-8 caprylic/capric glycerides, sorbitan olive oleate, polysorbate-20, laureth-23, PEG-40 stearate, steareth-21, palmitol and PEG-75 stearate and mixtures of glyceryl stearate and steareth-20, polysorbate-80, soya lecithin PC60, mixtures of glyceryl stearate and PEG-75 stearate, poloxamer 407, PEG-30 dipolyhydroxystearate, ceteareth-25;
the filler is selected from at least one of titanium dioxide, mica, boron nitride, polymethylsilsesquioxane, diatomite, kaolin, cellulose acetate, aluminum starch octylsuccinate, talcum powder, barium sulfate and volcanic ash;
the thickening agent is selected from at least one of hydroxyethyl cellulose, xanthan gum, carbomer, acrylic acid (ester)/copolymer, polymer EMT-10, polyacrylate cross-linked polymer-6, sodium acrylate/sodium acryloyldimethyl taurate copolymer, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, acryloyldimethyl taurate/VP copolymer, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, polyethylene and gum arabic;
the preservative is at least one selected from phenoxyethanol, ethylhexyl glycerol, pentanediol, hexanediol, aroyl ketone, sodium benzoate, triclosan and chlorphenesin.
6. The method for preparing the anti-aging mask composition according to any one of claims 1 to 5, wherein the preparation method comprises the following process steps:
1) wetting and completely dispersing the thickening agent in water to obtain a mixed solution;
2) adding DNA repair promoting ingredients, an emollient, a filler, a preservative and EDTA-2NA into the mixed solution obtained in the step 1), heating to 65 ℃, and homogenizing at 3000r/min for 3 minutes to completely disperse the mixed solution;
3) adding an emulsifier and a daily essence into the step 2), homogenizing at 3000r/min for 3 minutes to completely emulsify the mixture, and heating to 65 ℃ to obtain the mask composition with the DNA repairing effect.
7. A mask, characterized in that: the marine-derived skin DNA repair cell anti-aging cosmetic prepared by the preparation method of the anti-aging mask composition according to claim 6 has anti-aging, tension and tightening effects on facial skin, has a regulating effect on the expression of metallothionein, NQO1, Ki67 and MMP1, and has a reinforcing effect on a dermal structure by targeting type I collagen and hyaluronic acid.
8. The mask of claim 7, wherein: the cosmetic is in the form of a solvent dispersion or suspension, or in the form of emulsified particles, or in the form of a polymer coating.
9. The mask composition of marine-derived skin DNA repair cell anti-aging complex according to claim 1, wherein: the composition can also be applied to skin cream, skin lotion, skin gel, skin essence, skin lotion, eye cream, body lotion, gel or sunscreen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110312378.5A CN113143840A (en) | 2021-03-24 | 2021-03-24 | Facial mask composition containing marine-source skin DNA repair cell anti-aging compound and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114366698A (en) * | 2021-11-23 | 2022-04-19 | 湖北省麦诗特生物科技有限公司 | Anti-aging composition, preparation method thereof and application thereof in cosmetics |
| CN116473883A (en) * | 2023-05-22 | 2023-07-25 | 广州凡岛网络科技有限公司 | Anti-aging composition and preparation method and application thereof |
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| FR2838341A1 (en) * | 2002-04-10 | 2003-10-17 | Gelyma | Cosmetic or dermatological compositions, e.g. for combating skin aging, erythema or irritation, containing extract of Cystoseira stricta brown algae having e.g. DNA protecting action |
| WO2020139145A2 (en) * | 2018-12-29 | 2020-07-02 | Общество С Ограниченной Ответственностью "Сплат Глобал" | Cosmetic hair care product |
| CN112263524A (en) * | 2020-11-27 | 2021-01-26 | 湖北省麦诗特生物科技有限公司 | Cosmetic composition with multiple skin care effects and preparation method thereof |
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| FR2838341A1 (en) * | 2002-04-10 | 2003-10-17 | Gelyma | Cosmetic or dermatological compositions, e.g. for combating skin aging, erythema or irritation, containing extract of Cystoseira stricta brown algae having e.g. DNA protecting action |
| WO2020139145A2 (en) * | 2018-12-29 | 2020-07-02 | Общество С Ограниченной Ответственностью "Сплат Глобал" | Cosmetic hair care product |
| CN112263524A (en) * | 2020-11-27 | 2021-01-26 | 湖北省麦诗特生物科技有限公司 | Cosmetic composition with multiple skin care effects and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114366698A (en) * | 2021-11-23 | 2022-04-19 | 湖北省麦诗特生物科技有限公司 | Anti-aging composition, preparation method thereof and application thereof in cosmetics |
| CN114366698B (en) * | 2021-11-23 | 2024-01-26 | 湖北省麦诗特生物科技有限公司 | Anti-aging composition, preparation method thereof and application thereof in cosmetics |
| CN116473883A (en) * | 2023-05-22 | 2023-07-25 | 广州凡岛网络科技有限公司 | Anti-aging composition and preparation method and application thereof |
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Application publication date: 20210723 |