CN113142241A - 透明质酸杀螨剂 - Google Patents
透明质酸杀螨剂 Download PDFInfo
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- CN113142241A CN113142241A CN202010012069.1A CN202010012069A CN113142241A CN 113142241 A CN113142241 A CN 113142241A CN 202010012069 A CN202010012069 A CN 202010012069A CN 113142241 A CN113142241 A CN 113142241A
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- hyaluronic acid
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- acaricide
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- 229920002674 hyaluronan Polymers 0.000 title claims abstract description 28
- 229960003160 hyaluronic acid Drugs 0.000 title claims abstract description 28
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 title claims abstract description 20
- 230000000895 acaricidal effect Effects 0.000 title claims abstract description 15
- 239000000642 acaricide Substances 0.000 title claims abstract description 15
- 241000894006 Bacteria Species 0.000 claims abstract description 18
- 239000002207 metabolite Substances 0.000 claims abstract description 15
- 238000000855 fermentation Methods 0.000 claims abstract description 12
- 230000004151 fermentation Effects 0.000 claims abstract description 12
- -1 hyaluronic acid compound Chemical class 0.000 claims abstract description 8
- 241000223250 Metarhizium anisopliae Species 0.000 claims abstract description 7
- 230000000813 microbial effect Effects 0.000 claims abstract description 7
- 241000751139 Beauveria bassiana Species 0.000 claims abstract description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 6
- 241000194017 Streptococcus Species 0.000 claims abstract description 6
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 239000008103 glucose Substances 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims abstract description 6
- 239000012535 impurity Substances 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract description 6
- 238000001556 precipitation Methods 0.000 claims abstract description 6
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 5
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 241000186660 Lactobacillus Species 0.000 claims description 2
- 239000012752 auxiliary agent Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 229940039696 lactobacillus Drugs 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims description 2
- 241000238876 Acari Species 0.000 abstract description 6
- 241000607479 Yersinia pestis Species 0.000 abstract description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 6
- 235000014655 lactic acid Nutrition 0.000 abstract description 3
- 239000004310 lactic acid Substances 0.000 abstract description 3
- 241001124076 Aphididae Species 0.000 abstract description 2
- 241001466042 Fulgoromorpha Species 0.000 abstract description 2
- 241000488583 Panonychus ulmi Species 0.000 abstract description 2
- 239000002131 composite material Substances 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
透明质酸杀螨剂，微生物透明质酸复合菌代谢物发酵以葡萄糖作为碳原发酵液，在培养基中发酵48小时，发酵结束后，过滤除去菌丝体和杂质，然后采用醇沉淀法得到高纯度的透明质酸复合菌代谢物。本发明能按照所需产品功能来设定分子量大小，同时根据产品的特殊性优化出链球菌、乳酸菌、金龟子绿僵菌、球孢白僵菌、苏云金杆菌复合而成的代谢产物，用于植物杀螨，阻塞螨类、红蜘蛛、蚜虫、飞虱等害虫的气孔，导致害虫死亡。
Description
技术领域
本发明涉及药剂技术领域，具体为透明质酸杀螨剂。
背景技术
螨类在形态、习性及栖息场所等方面具有多样性，其分布广、适应性强，种类繁多，可以危害150多种作物，每年都给农林业造成极大的损失，同时螨类繁殖能力强，极易产生抗性，使常规杀螨剂药效降低。
发明内容
本发明针对现有技术的不足，提出透明质酸杀螨剂，可作为农用杀螨剂、杀菌剂喷雾使用，能快速杀螨，杀虫，提高生物活性，还可阻塞害虫气孔，导致害虫死亡。
为实现上述发明目的，本发明提供技术方案：透明质酸杀螨剂，其特征在于：由微生物透明质酸复合菌代谢物与特殊助剂配制而成。
进一步地，微生物透明质酸复合菌包括链球菌、乳酸菌、金龟子绿僵菌、球孢白僵菌、苏云金杆菌。
进一步地，微生物透明质酸复合菌代谢物发酵以葡萄糖作为碳原发酵液，在培养基中发酵48小时，发酵结束后，过滤除去菌丝体和杂质，然后采用醇沉淀法得到高纯度的透明质酸复合菌代谢物。
进一步地，微生物透明质酸复合菌代谢物为无色透明可分散水剂。
与现有技术相比，本发明具有以下优点：能按照所需产品功能来设定分子量大小，同时根据产品的特殊性优化出链球菌、乳酸菌、金龟子绿僵菌、球孢白僵菌、苏云金杆菌复合而成的代谢产物，用于植物杀螨，阻塞螨类、红蜘蛛、蚜虫、飞虱等害虫的气孔，导致害虫死亡。
具体实施方式
下面结合实施例对本发明进行详细描述，本部分的描述仅是示范性和解释性，不应对本发明的保护范围有任何的限制作用。
实施例1
透明质酸杀螨剂，以葡萄糖作为碳原发酵液，链球菌、乳酸菌、金龟子绿僵菌为菌种在培养基中发酵48小时，发酵结束后，过滤除去菌丝体和杂质，然后采用醇沉淀法得到高纯度的透明质酸复合菌代谢物。
实施例2
透明质酸杀螨剂，以葡萄糖作为碳原发酵液，金龟子绿僵菌、球孢白僵菌、苏云金杆菌为菌种在培养基中发酵52小时，发酵结束后，过滤除去菌丝体和杂质，然后采用醇沉淀法得到高纯度的透明质酸复合菌代谢物。
实施例3
透明质酸杀螨剂，以葡萄糖作为碳原发酵液，链球菌、金龟子绿僵菌、球孢白僵菌为菌种在培养基中发酵54小时，发酵结束后，过滤除去菌丝体和杂质，然后采用醇沉淀法得到高纯度的透明质酸复合菌代谢物。
Claims (4)
1.透明质酸杀螨剂，其特征在于：由微生物透明质酸复合菌代谢物与特殊助剂配制而成。
2.根据权利要求1所述的透明质酸杀螨剂，其特征在于：微生物透明质酸复合菌包括链球菌、乳酸菌、金龟子绿僵菌、球孢白僵菌、苏云金杆菌。
3.根据权利要求1所述的透明质酸杀螨剂，其特征在于：微生物透明质酸复合菌代谢物发酵以葡萄糖作为碳原发酵液，在培养基中发酵48小时，发酵结束后，过滤除去菌丝体和杂质，然后采用醇沉淀法得到高纯度的透明质酸复合菌代谢物。
4.根据权利要求1所述的透明质酸杀螨剂，其特征在于：微生物透明质酸复合菌代谢物为无色透明可分散水剂。
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010012069.1A CN113142241A (zh) | 2020-01-07 | 2020-01-07 | 透明质酸杀螨剂 |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010012069.1A CN113142241A (zh) | 2020-01-07 | 2020-01-07 | 透明质酸杀螨剂 |
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| CN113142241A true CN113142241A (zh) | 2021-07-23 |
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| CN202010012069.1A Pending CN113142241A (zh) | 2020-01-07 | 2020-01-07 | 透明质酸杀螨剂 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5211946A (en) * | 1991-09-13 | 1993-05-18 | Mycogen Corporation | Bacillus thuringiensis isolates for controlling acarides |
| CN101338279A (zh) * | 2008-05-07 | 2009-01-07 | 浙江大学 | 丝孢类真菌杀螨制剂 |
| CN101790976A (zh) * | 2010-03-18 | 2010-08-04 | 温州医学院 | 一种植物叶面光亮清洁剂 |
| JP2010252641A (ja) * | 2009-04-22 | 2010-11-11 | Mitsubishi Rayon Co Ltd | 乳酸菌由来多糖類の製造方法 |
| CN104693320A (zh) * | 2015-03-31 | 2015-06-10 | 广西还珠海洋生物科技有限公司 | 一种从海蜇中提取透明质酸的方法 |
-
2020
- 2020-01-07 CN CN202010012069.1A patent/CN113142241A/zh active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5211946A (en) * | 1991-09-13 | 1993-05-18 | Mycogen Corporation | Bacillus thuringiensis isolates for controlling acarides |
| CN101338279A (zh) * | 2008-05-07 | 2009-01-07 | 浙江大学 | 丝孢类真菌杀螨制剂 |
| JP2010252641A (ja) * | 2009-04-22 | 2010-11-11 | Mitsubishi Rayon Co Ltd | 乳酸菌由来多糖類の製造方法 |
| CN101790976A (zh) * | 2010-03-18 | 2010-08-04 | 温州医学院 | 一种植物叶面光亮清洁剂 |
| CN104693320A (zh) * | 2015-03-31 | 2015-06-10 | 广西还珠海洋生物科技有限公司 | 一种从海蜇中提取透明质酸的方法 |
Non-Patent Citations (1)
| Title |
|---|
| 姜锡瑞 等主编, 中国轻工业出版社 * |
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