CN113088486A - 用于分离贴壁细胞的无酶解离液及其制备方法 - Google Patents
用于分离贴壁细胞的无酶解离液及其制备方法 Download PDFInfo
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- CN113088486A CN113088486A CN202110233559.9A CN202110233559A CN113088486A CN 113088486 A CN113088486 A CN 113088486A CN 202110233559 A CN202110233559 A CN 202110233559A CN 113088486 A CN113088486 A CN 113088486A
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- enzyme
- adherent cells
- free dissociation
- separating
- arginine
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- C—CHEMISTRY; METALLURGY
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0623—Stem cells
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0656—Adult fibroblasts
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
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- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/62—Plastics recycling; Rubber recycling
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Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202110233559.9A CN113088486B (zh) | 2021-03-03 | 2021-03-03 | 用于分离贴壁细胞的无酶解离液及其制备方法 |
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CN202110233559.9A CN113088486B (zh) | 2021-03-03 | 2021-03-03 | 用于分离贴壁细胞的无酶解离液及其制备方法 |
Publications (2)
Publication Number | Publication Date |
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CN113088486A true CN113088486A (zh) | 2021-07-09 |
CN113088486B CN113088486B (zh) | 2023-06-16 |
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CN202110233559.9A Active CN113088486B (zh) | 2021-03-03 | 2021-03-03 | 用于分离贴壁细胞的无酶解离液及其制备方法 |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107557328A (zh) * | 2017-10-31 | 2018-01-09 | 妙顺(上海)生物科技有限公司 | 一种用于分离贴壁细胞的消化液及其分离方法 |
US20190031990A1 (en) * | 2016-01-26 | 2019-01-31 | Centre For Commercialization Of Regenerative Medicine | Method for dissociating cell aggregates |
JP2019024422A (ja) * | 2017-07-31 | 2019-02-21 | 国立大学法人広島大学 | 細胞解離剤、細胞解離方法および細胞シートの製造方法 |
CN109439621A (zh) * | 2018-11-12 | 2019-03-08 | 王晓柯 | 一种干细胞温和酶细胞消化液 |
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2021
- 2021-03-03 CN CN202110233559.9A patent/CN113088486B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20190031990A1 (en) * | 2016-01-26 | 2019-01-31 | Centre For Commercialization Of Regenerative Medicine | Method for dissociating cell aggregates |
JP2019024422A (ja) * | 2017-07-31 | 2019-02-21 | 国立大学法人広島大学 | 細胞解離剤、細胞解離方法および細胞シートの製造方法 |
CN107557328A (zh) * | 2017-10-31 | 2018-01-09 | 妙顺(上海)生物科技有限公司 | 一种用于分离贴壁细胞的消化液及其分离方法 |
CN109439621A (zh) * | 2018-11-12 | 2019-03-08 | 王晓柯 | 一种干细胞温和酶细胞消化液 |
Non-Patent Citations (1)
Title |
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TAKESHI IKEDA 等: "Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish", 《BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY》 * |
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CN113088486B (zh) | 2023-06-16 |
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Effective date of registration: 20230710 Address after: 528315 room 716, 7 / F, building A1, Sino Europe service center, No.2 South Lingnan Avenue, xingle community residents committee, Lecong Town, Shunde District, Foshan City, Guangdong Province Patentee after: Guangdong Weixiang Biotechnology Co.,Ltd. Patentee after: Guangdong Yashan Biotechnology Co.,Ltd. Address before: 528315 room 716, 7 / F, building A1, Sino Europe service center, No.2 South Lingnan Avenue, xingle community residents committee, Lecong Town, Shunde District, Foshan City, Guangdong Province Patentee before: Guangdong Weixiang Biotechnology Co.,Ltd. |