CN113068609B - Method for cultivating new potted flower variety by utilizing silver She Lanzhong flowers and fine She Lanzhong flowers - Google Patents
Method for cultivating new potted flower variety by utilizing silver She Lanzhong flowers and fine She Lanzhong flowers Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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Abstract
The invention discloses a method for cultivating a new variety of potted flowers by utilizing silver She Lanzhong flowers and fine She Lanzhong flowers. The method comprises the steps of taking female flowers of silver She Lanzhong flowers as female parents and amphoteric flowers of fine She Lanzhong flowers as male parents, or taking female flowers of fine leaves Lan Zhonghua as female parents and amphoteric flowers of silver She Lanzhong flowers as male parents, carrying out artificial pollination, collecting F1 hybrid seeds, sowing the F1 hybrid seeds, culturing seedlings and maintaining. The invention utilizes the silver She Lanzhong flowers and the fine She Lanzhong flowers of the blue-bell flower plant to cultivate a new variety of blue Zhong Huapen flowers suitable for potting for the first time, and provides specific hybridization measures. By adopting the method, on the basis of keeping the advantages (blue flowers) of the original parent, the bad characters (easy breakage of branches, tiny plants and the like) of the original parent are improved, and the thick plants, difficult breakage of branches, large flowers, greatly improved ornamental value and high transplanting survival rate are obtained.
Description
Technical Field
The invention relates to the technical field of plant cross breeding, in particular to a method for cultivating a new potted flower variety by utilizing silver She Lanzhong flowers and fine She Lanzhong flowers.
Background
The new excellent peculiarities of garden plants are always pursued by the world. Blue garden plants are popular because of elegant and unique flower colors and scarcity of germplasm resources [1] . China has a lot of rich blue flower plant resources, but the precious resources can only bloom to the mountain due to long-term scientific research and little investment. In recent years, the household gardening industry has been developed and developed gradually due to the improvement of the living standard and income of people [2] . However, the level of research and development of flower resources, which is seriously lagged in China, is not consistent with the home gardening industry which is vigorously developed in China. To meet the increasing demand of market for new and odd flowers, all large flower enterprises in China walk to all international gardening expounds to search new and odd flower varieties. However, the introduction of new foreign varieties is not only limited by people, but also requires paying high-priced patentsAnd (5) cost. Under the background, the original innovation is started by utilizing the excellent wild resources in the nature of China, the novel flower variety with breakthrough is bred, the speaking right of the novel flower variety is striven for, and the method has extremely important significance.
Lan Zhonghua it is a plant of the genus Campanulaceae (Campanulaceae) Lan Zhonghua (Cyananthus). Lan Zhonghua plants are typical components of the China-Himalayan system [3] Mainly distributed in Yunnan et al, about 28 species worldwide, wherein only Yunnan province has 19 11 varieties, 14 species (including varieties) are characteristic species [4] . The plant of the genus is grown on the roadside sun-facing Dan Po land in a paving mode, and is extremely drought-resistant and barren. Lan Zhonghua has gorgeous corolla and luxuriant flower branches, has high ornamental value, and has wide development prospect when being used as garden plants. For a long time, the genus blue-bell has been present in a wild state. The research reports are less, only the resource investigation introduction reports are found [4、5、6] 。
Silver She Lanzhong flowers (Cyananthus argenteus), also known as total flowers Lan Zhonghua, are specifically produced in Yunnan, belong to the perennial subgroup of the blue flowers group (Sect. Stenolobi) of the genus blue, which are in the form of oval needles, corolla bluish purple, and have both female and amphoteric plants within the flower population of silver She Lanzhong [6] . However, the stem is not branched or has a plurality of short branches, is extremely easy to break and is not transplanting-resistant, and the development and utilization of silver She Lanzhong flower resources are severely limited. The fine She Lanzhong flowers (Cyananthus delavayi) and the silver She Lanzhong flowers are the same subgroup, the leaves of which are nearly round or wide oval triangle, the corolla is dark blue, and Yunnan and Sichuan are produced. The fine She Lanzhong flower population also has female plants and amphoteric plants [7] . The thin She Lanzhong flower plants are luxuriant, the stems are thin and branched, and the flowers are not easy to break, but the stems are short, the flowers are fine, and the ornamental value is poor. The development of a new blue Zhong Huapen flower variety which is not easy to break and large in flower and is cultivated by silver She Lanzhong flowers and fine She Lanzhong flowers, can realize the original innovation while exploring excellent blue flower plant resources in China, and fundamentally relieves the dilemma of lack of autonomous new varieties in China.
Reference is made to:
[1] wang Jinning application of blue flowers in urban gardens-take Nanjing China Afforestation exposition garden as an example, shenzhen institute of occupational technology, 2016,5 (03): 62-68
[2] Cui Jianying, wang Yushu, background and current situation of domestic gardening industry, agricultural science of Heilongjiang, 2020, (07): 116-118
[3] Hong Deyuan, ma Liming, lan Zhonghua, journal of plant classification, 1991, 29 (01): 25-51
[4] Jie Weijia et al, probex for the investigation of plant resources of the genus blue-flower in Yunnan province, proc. Agricultural science in southwest, 2009, 22 (01): 173-176
[5] Zhou Mengxia, et al, sichuan blue-flower resource investigation and garden application research, chinese wild plant resource 2016, 35 (03): 53-54
[6] Lu Gongchun, jie Wei, flowering dynamic study of female flowers and amphoteric flowers of silver She Lanzhong flowers, jiangxi agricultural journal, 2016, 28 (04): 19-22
[7] Wang Hao, fine She Lanzhong flowers (Cyananthus delavayi) female disparate plant system maintained ecological mechanism, university of Sichuan agriculture, 2017-06-01, university of Shuoshi treatises
Disclosure of Invention
In order to realize the protection, development and utilization of wild resources in China and effectively solve the technical problems of lack of new blue flower varieties, tiny flowers, extremely easy breakage of branches and poor ornamental value in China, the invention provides a method for cultivating new potted flower varieties by utilizing silver She Lanzhong flowers and fine She Lanzhong flowers so as to promote the protection, development and utilization of blue flower plant resources in China and realize independent innovation of new varieties.
The technical scheme of the invention is as follows:
a method for cultivating new variety of potted flower by utilizing silver She Lanzhong flower and fine She Lanzhong flower includes artificial pollination by using female flower of silver She Lanzhong flower as female parent and amphoteric flower of fine She Lanzhong flower as male parent, collecting F1 hybrid seed and storing, sowing F1 hybrid seed, raising seedling and curing to obtain new variety of blue Zhong Huapen flower.
Further, the artificial pollination comprises:
(1) Hybridization bagging: selecting full and compact buds on silver She Lanzhong flower female flower plants, and bagging the buds by using a sulfuric acid paper bag; simultaneously, selecting full and compact buds on the fine leaf Lan Zhonghua amphoteric flower plants, and bagging the buds by using a sulfuric acid paper bag;
(2) Artificial pollination: 4-5 d after the flower buds of the silver She Lanzhong flower female flowers of the bagging are opened, the stigmas of the flower buds are cracked, the edges of the split blades start to be bent back, mucus is secreted on the surfaces of the flower buds, when the flower petals also start to be bent back, and when the flower buds of the fine leaf Lan Zhong amphoteric flowers of the bagging are opened and the flower stigmas are fully distributed on the spherical upper surfaces of the centers of the flower cylinders, pollen is taken out and smeared on the stigmas of the silver She Lanzhong flower female flowers of the bagging, so that the stigmas are fully stained with pollen, and artificial pollination is completed; the female flowers of the silver She Lanzhong flowers after pollination are continuously sleeved with the sulfuric acid paper bags, and the thin She Lanzhong flowers are the amphoteric flowers, so that the sulfuric acid paper bags are removed;
(3) Removing the bag of silver She Lanzhong flower: and 7-14 days after pollination, removing the sulfuric acid paper bag on the silver She Lanzhong flower female flowers when the flower column heads of the silver She Lanzhong flower female flowers of the bagging wither.
Further, the F1 hybrid seeds are collected and stored, namely, fruits of the pollinated flowers are picked when the ovaries of the pollinated flowers turn yellow in 10-11 months of the year of artificial pollination, the hybrid seeds are peeled off, and then the hybrid seeds are placed in paper bags for storage at room temperature.
Further, the seedling raising and maintenance of the F1 hybrid seeds are carried out in 3-4 months of the next year of artificial pollination, a cultivation substrate is prepared according to the volume ratio of peat to perlite=1:1 and is arranged in a seedling raising hole tray, the F1 hybrid seeds are pressed into the cultivation substrate according to holes, the humidity of the cultivation substrate is kept at 60-80% during the seedling raising, and the room temperature is 20-25 ℃; and (3) after 15-20 days of sowing, F1 hybrid seeds germinate, and under the conventional maintenance, the seedlings bloom in 7-8 months of the current year of sowing to obtain a new flowering blue Zhong Huapen flower variety.
The invention also provides another method for cultivating new potted flower varieties by utilizing silver She Lanzhong flowers and fine She Lanzhong flowers, which comprises the steps of taking female flowers of fine leaves Lan Zhonghua as female parent and amphoteric flowers of silver She Lanzhong flowers as male parent, performing artificial pollination, collecting F1 hybrid seeds, storing, sowing the F1 hybrid seeds, raising seedlings and maintaining to obtain new blue Zhong Huapen flower varieties in 8-9 months.
Further, in another provided method for cultivating a new variety of potted flowers using silver She Lanzhong flowers and fine She Lanzhong flowers, the artificial pollination comprises:
(1) Hybridization bagging: selecting full and compact buds on the female flower plants of the fine leaves Lan Zhonghua, and bagging the buds by using a sulfuric acid paper bag; simultaneously, full and compact buds on the silver She Lanzhong flower amphoteric flower plants are selected, and are covered by a sulfuric acid paper bag;
(2) Artificial pollination: 4-5 d after the flower buds of the fine leaf Lan Zhonghua female flowers of the bagging are opened, the stigmas of the flowers are cracked, the edges of the split leaves are bent after starting, mucus is secreted on the surfaces of the flowers, when the flower buds of the silver She Lanzhong amphoteric flowers of the bagging are opened and the stigmas are fully distributed on the spherical upper surfaces of the centers of the flower cylinders, the pollen is taken and smeared on the stigmas of the fine leaf Lan Zhonghua female flowers of the bagging, the stigmas of the flowers are fully stained with the pollen, and then artificial pollination is completed; the pollinated fine leaf Lan Zhonghua female flowers are continuously sleeved with a sulfuric acid paper bag, and the silver She Lanzhong flower amphoteric flowers are removed from the sulfuric acid paper bag;
(3) Bag removal of fine She Lanzhong flowers: and 7-14 days after pollination, removing the sulfuric acid paper bag on the female flowers of the fine She Lanzhong flowers when the female flower heads of the fine leaves Lan Zhonghua of the bagging wither.
Further, in the provided method for cultivating new potted flower varieties by utilizing silver She Lanzhong flowers and fine She Lanzhong flowers, the F1 hybrid seeds are collected and stored in a paper bag after the fruits of the flowers are picked when the pollinated flowers turn yellow in 10-11 months of the current year of artificial pollination, and the hybrid seeds are placed in the paper bag for storage at room temperature.
Further, in the provided another method for cultivating new potted flower varieties by utilizing silver She Lanzhong flowers and fine She Lanzhong flowers, the sowing F1 hybrid seed seedling cultivation maintenance is to prepare a cultivation substrate according to the volume ratio of peat to perlite=1:1 to be arranged on a seedling cultivation hole tray in 3-4 months of the next year of artificial pollination, the F1 hybrid seeds are pressed into the cultivation substrate according to holes, the humidity of the cultivation substrate is kept at 60-80% during the seedling cultivation, and the room temperature is 20-25 ℃. And (3) germinating the F1 hybrid seeds 15-20 days after sowing, and under the conventional maintenance, flowering the seedlings in 7-8 months of the sowing year to obtain a novel flowering blue Zhong Huapen flower variety.
Compared with the prior art, the invention has the beneficial effects that:
the parent silver She Lanzhong flower is large, the diameter of the flower is 2.00-2.30 cm, the stem is not branched or has a plurality of short branches, and the flower is extremely easy to break, the transplanting breaking rate reaches 30-50%, and the flower is difficult to transplant; the parent flower is fine She Lanzhong, the diameter of the flower is 1.00-1.50 cm, the stem is thin and branched, and is not easy to break, but the stem is short, and the diameter of the flower is only 10-20 cm. The invention utilizes the silver She Lanzhong flowers and the fine She Lanzhong flowers of the blue-bell flower plant to cultivate a new variety of blue Zhong Huapen flowers suitable for potting for the first time, and provides specific hybridization measures. The method improves the bad characters of the original parent (the stems are not branched or less branched and are easy to break, the stems are short and the flowers are small) on the basis of keeping the advantages of the original parent (blue flowers), obtains the thick plant, the stems are 20-25 cm long, the branches are difficult to break, the breaking rate is as low as 0-5%, the average stems are 10-12, the average branches on the stems are 5-8, the plant type is attractive, the number of branched flowers is large, the average flower setting rate of the branches is 2-3, the number of the single plant flowers is 100-290, the flowers are large (when the silver She Lanzhong flower female flowers of the large flowers are used as female parents, compared with the female parent flowers, the diameter of the smallest flowers cultivated by the method is not smaller than the diameter of the female parent, the diameter of the large flowers cultivated by taking the thin leaves Lan Zhonghua of the small flowers as female parents, compared with the female parent flowers, the smallest flowers are larger than the female parent flowers, and the ornamental value is greatly improved, and the potted plant is suitable. The new blue Zhong Huapen flower variety cultivated by the method is easy to transplant (the breaking rate is as low as 0-5%).
Detailed Description
Example 1
(1) Selection of hybrid parents: during 8-9 months, female flowers of silver She Lanzhong flowers (namely female flowers with abortive stamens) are taken as female parents, and amphoteric flowers of fine leaves Lan Zhonghua (namely amphoteric flowers with normal development of male and female stamens) are taken as male parents for artificial pollination.
(2) Hybridization bagging: selecting full and compact buds on silver She Lanzhong flower female flower plants, and bagging the buds by using a sulfuric acid paper bag; simultaneously, selecting full and compact flower buds of the fine leaf Lan Zhonghua amphoteric flower plants, and bagging the flower buds by using a sulfuric acid paper bag;
(3) Hybridization pollination: 4-5 d after the flower buds of the silver She Lanzhong flower female flowers of the bagging are opened, the stigmas of the flower buds are cracked, the edges of the split leaves are bent after starting, mucus is secreted on the surfaces of the flower buds, when the petals are bent after starting, and when the flower buds of the fine leaf Lan Zhong amphoteric flowers of the bagging are opened and the flower stigmas are fully distributed on the spherical upper surfaces of the centers of the flower cylinders, pollen is taken out and smeared on the stigmas of the silver She Lanzhong flower female flowers of the bagging, so that the stigmas of the flower buds are fully stained with pollen, and artificial pollination is completed; the female flowers of the silver She Lanzhong flowers after pollination are continuously sleeved with the sulfuric acid paper bags, and the thin She Lanzhong flowers are the amphoteric flowers, so that the sulfuric acid paper bags are removed;
(4) Removing the bag of silver She Lanzhong flower: and 7-14 days after pollination, removing the sulfuric acid paper bag on the silver She Lanzhong flower female flowers when the flower column heads of the silver She Lanzhong flower female flowers of the bagging wither.
(5) And (5) seed collection and seed storage: picking fruits of pollinated flowers when their ovaries turn yellow in the current year of artificial pollination for 10-11 months, stripping out the hybrid seeds to obtain F1 hybrid seeds, placing the F1 hybrid seeds in paper bags, and storing at room temperature of 20-25 ℃.
(6) And (3) seedling cultivation and maintenance: in 3-4 months of the next year of artificial pollination, preparing a culture medium according to the volume ratio of peat to perlite=1:1, loading the culture medium into a seedling culture tray, pressing F1 hybrid seeds into the culture medium according to the holes (sowing), and keeping the humidity of the culture medium at 60-80% and the room temperature at 20-25 ℃. And the F1 hybrid seeds germinate 15-20 days after sowing. Under the conventional maintenance, the seedlings bloom in 7-8 months of the current year after sowing, namely the novel flowering blue Zhong Huapen variety. The conventional curing includes: 1 water is poured 1 time on sunny days 1-2 days, 1 water is poured 1 time on cloudy days 2-3 days, and 1 time of 1000 times of liquid fertilizer is sprayed on a growth season of 3-8 months each month, wherein the liquid fertilizer contains N20 percent and P in percentage by mass 2 O 5 20％，K 2 O 20％。
Example 2
(1) Selection of hybrid parents: during 8-9 months, female flowers of fine leaves Lan Zhonghua (namely female flowers with abortive stamens) are taken as female parents, and amphoteric flowers of silver She Lanzhong flowers (namely amphoteric flowers with normal development of male and female cores) are taken as male parents for artificial pollination.
(2) Hybridization bagging: selecting full and compact buds on the female flower plants of the fine leaves Lan Zhonghua, and bagging the buds by using a sulfuric acid paper bag; simultaneously, selecting full and compact flower buds of the silver She Lanzhong flower amphiprotic flower plants, and bagging the flower buds by using a sulfuric acid paper bag;
(3) Artificial pollination: 4-5 d after the flower buds of the fine leaf Lan Zhonghua female flowers of the bagging are opened, the stigmas of the flower buds are cracked, the edges of the split blades start to be bent back, mucus is secreted on the surfaces of the flower buds, when the flower petals start to be bent back, and when the flower buds of the silver She Lanzhong amphoteric flowers of the bagging are opened and the flower stigmas are fully distributed on the spherical upper surfaces of the centers of the flower cylinders, pollen is taken out and smeared on the stigmas of the fine leaf Lan Zhonghua female flowers of the bagging, the stigmas of the flower stigmas are fully stained with the pollen, and then artificial pollination is completed. The pollinated fine leaf Lan Zhonghua female flowers are continuously sleeved with a sulfuric acid paper bag, and the sulfuric acid paper bag is removed from the silver She Lanzhong flower amphoteric flowers;
(4) Bag removal of fine She Lanzhong flowers: and 7-14 days after pollination, removing the sulfuric acid paper bag on the female flowers of the fine She Lanzhong flowers when the female flower heads of the fine leaves Lan Zhonghua of the bagging wither.
(5) And (5) seed collection and seed storage: picking fruits of pollinated flowers when their ovaries turn yellow in 10-11 months of the current year of artificial pollination, stripping out the hybrid seeds to obtain F1 hybrid seeds, and placing the F1 hybrid seeds in paper bags for storage at room temperature of 20-25 ℃.
(6) And (3) seedling cultivation and maintenance: in 3-4 months of the next year of artificial pollination, preparing a culture medium according to the volume ratio of peat to perlite=1:1, loading the culture medium into a seedling culture tray, pressing F1 hybrid seeds into the culture medium according to the holes (sowing), and keeping the humidity of the culture medium at 60-80% and the room temperature at 20-25 ℃. And after sowing, the F1 hybrid seeds germinate for 15-20 days. Under the conventional curing (the conventional curing is the same as that of the example 1), the seedlings bloom in 7-8 months of the current year of sowing, and the seedlings are the new flowering blue Zhong Huapen flower variety.
According to the invention, a novel blue Zhong Huapen flower variety with excellent characters is obtained, and the specific characters are compared with the following table 1.
TABLE 1 comparison of the traits of silver She Lanzhong flowers and fine She Lanzhong flowers and their hybrid F1 offspring
Claims (6)
1. A method for cultivating a new potted flower variety by utilizing silver She Lanzhong flowers and fine She Lanzhong flowers is characterized in that female flowers of silver She Lanzhong flowers are used as female parent and amphoteric flowers of fine She Lanzhong flowers are used as male parent in 8-9 months, artificial pollination is carried out, F1 hybrid seeds are collected and stored, and the new blue Zhong Huapen flower variety is obtained by sowing the F1 hybrid seeds, raising seedlings and maintaining;
the artificial pollination comprises:
(1) Hybridization bagging: selecting full and compact buds on silver She Lanzhong flower female flower plants, and bagging the buds by using a sulfuric acid paper bag; simultaneously, selecting full and compact buds on the fine leaf Lan Zhonghua amphoteric flower plants, and bagging the buds by using a sulfuric acid paper bag;
(2) Artificial pollination: 4-5 d after the flower buds of the silver She Lanzhong flower female flowers of the bagging are opened, the stigmas of the flower buds are cracked, the edges of the split blades start to be bent back, mucus is secreted on the surfaces of the flower buds, when the flower petals also start to be bent back, and when the flower buds of the fine leaf Lan Zhong amphoteric flowers of the bagging are opened and the flower stigmas are fully distributed on the spherical upper surfaces of the centers of the flower cylinders, pollen is taken out and smeared on the stigmas of the silver She Lanzhong flower female flowers of the bagging, so that the stigmas are fully stained with pollen, and artificial pollination is completed; the female flowers of the silver She Lanzhong flowers after pollination are continuously sleeved with the sulfuric acid paper bags, and the thin She Lanzhong flowers are the amphoteric flowers, so that the sulfuric acid paper bags are removed;
(3) Removing the bag of silver She Lanzhong flower: and 7-14 days after pollination, removing the sulfuric acid paper bag on the silver She Lanzhong flower female flowers when the flower column heads of the silver She Lanzhong flower female flowers of the bagging wither.
2. The method for cultivating new potted flower variety with silver She Lanzhong flower and fine She Lanzhong flower according to claim 1, wherein the collecting and storing of the F1 hybrid seed is to collect the fruit of the pollinated flower when the ovary of the pollinated flower turns yellow 10-11 months in the current year of artificial pollination, and to peel off the hybrid seed, and then to put the hybrid seed in a paper bag for storage at room temperature.
3. The method for cultivating new potted flower variety using silver She Lanzhong flowers and fine She Lanzhong flowers as claimed in claim 1, wherein
The method is characterized in that the seedling raising and maintenance of the F1 hybrid seeds are carried out in 3-4 months of the next year of artificial pollination, a culture medium is prepared according to the volume ratio of peat to perlite=1:1 and is arranged in a seedling raising hole tray, the F1 hybrid seeds are pressed into the culture medium according to holes, the humidity of the culture medium is kept at 60-80% during the seedling raising, and the room temperature is 20-25 ℃; and (3) after 15-20 days of sowing, F1 hybrid seeds germinate, and under the conventional maintenance, the seedlings bloom in 7-8 months of the current year of sowing to obtain a new flowering blue Zhong Huapen flower variety.
4. A method for cultivating a new potted flower variety by utilizing silver She Lanzhong flowers and fine She Lanzhong flowers is characterized in that female flowers of fine leaves Lan Zhonghua are used as female parents and amphoteric flowers of silver She Lanzhong flowers are used as male parents for artificial pollination in 8-9 months, F1 hybrid seeds are collected and stored, and the new blue Zhong Huapen flower variety is obtained by sowing the F1 hybrid seeds, raising seedlings and maintaining;
the artificial pollination comprises:
(1) Hybridization bagging: selecting full and compact buds on the female flower plants of the fine leaves Lan Zhonghua, and bagging the buds by using a sulfuric acid paper bag; simultaneously, full and compact buds on the silver She Lanzhong flower amphoteric flower plants are selected, and are covered by a sulfuric acid paper bag;
(2) Artificial pollination: 4-5 d after the flower buds of the fine leaf Lan Zhonghua female flowers of the bagging are opened, the stigmas of the flowers are cracked, the edges of the split leaves are bent after starting, mucus is secreted on the surfaces of the flowers, when the flower buds of the silver She Lanzhong amphoteric flowers of the bagging are opened and the stigmas are fully distributed on the spherical upper surfaces of the centers of the flower cylinders, the pollen is taken and smeared on the stigmas of the fine leaf Lan Zhonghua female flowers of the bagging, the stigmas of the flowers are fully stained with the pollen, and then artificial pollination is completed; the pollinated fine leaf Lan Zhonghua female flowers are continuously sleeved with a sulfuric acid paper bag, and the silver She Lanzhong flower amphoteric flowers are removed from the sulfuric acid paper bag;
(3) Bag removal of fine She Lanzhong flowers: and 7-14 days after pollination, removing the sulfuric acid paper bag on the female flowers of the fine She Lanzhong flowers when the female flower heads of the fine leaves Lan Zhonghua of the bagging wither.
5. The method for cultivating new potted flower variety with silver She Lanzhong flower and fine She Lanzhong flower according to claim 4, wherein the collecting and storing of the F1 hybrid seed is to collect the fruit of the pollinated flower when it turns yellow 10-11 months in the year of artificial pollination, and to put the hybrid seed in a paper bag after peeling off the hybrid seed, and to store at room temperature.
6. The method for cultivating new potted flower variety with silver She Lanzhong flower and fine She Lanzhong flower according to claim 4, wherein the sowing of F1 hybrid seed for cultivating and maintaining is characterized in that the cultivation substrate is prepared by the volume ratio of peat to perlite=1:1 in 3-4 months of artificial pollination, the F1 hybrid seed is put in the cultivation cavity plate, the F1 hybrid seed is pressed into the cultivation substrate according to the cavity, the humidity of the cultivation substrate is kept at 60-80% during the cultivation, and the room temperature is 20-25 ℃; and after 15 to 20 days of sowing, F1 hybrid seeds germinate, and under the conventional maintenance, the seedlings bloom in 7 to 8 months of the sowing year to obtain a new flowering blue Zhong Huapen flower variety.
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